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Lumazine Synthase Protein Nanoparticle-Gd(III)-DOTA Conjugate as a T1 contrast agent for high-field MRI

Abstract

With the applications of magnetic resonance imaging (MRI) at higher magnetic fields increasing, there is demand for MRI contrast agents with improved relaxivity at higher magnetic fields. Macromolecule-based contrast agents, such as protein-based ones, are known to yield significantly higher r1 relaxivity at low fields, but tend to lose this merit when used as T1 contrast agents (r1/r2 = 0.5 ~ 1), with their r1 decreasing and r2 increasing as magnetic field strength increases. Here, we developed and characterized an in vivo applicable magnetic resonance (MR) positive contrast agent by conjugating Gd(III)-chelating agent complexes to lumazine synthase isolated from Aquifex aeolicus (AaLS). The r1 relaxivity of Gd(III)-DOTA-AaLS-R108C was 16.49 mM−1s−1 and its r1/r2 ratio was 0.52 at the magnetic field strength of 7 T. The results of 3D MR angiography demonstrated the feasibility of vasculature imaging within 2 h of intravenous injection of the agent and a significant reduction in T1 values were observed in the tumor region 7 h post-injection in the SCC-7 flank tumor model. Our findings suggest that Gd(III)-DOTA-AaLS-R108C could serve as a potential theranostic nanoplatform at high magnetic field strength.

Introduction

With the applications of MRI in higher magnetic fields (>7 T) both in preclinical and clinical settings increasing, there are needs to develop MRI contrast agents with improved relaxivity at higher magnetic fields. From MRI perspective, the advantage of higher magnetic field is attractive, as it is expected to drastically increase signal to noise ratio (SNR) and resolution1,2. However, it is generally observed that spin-lattice time (T1) increases and converges for different tissues as the magnetic field strength increases, which reduces contrast for T1-weighted exams using the same imaging parameters used at conventional 1.5 or 3 T3,4. As contrast to noise ratio (CNR) is critical for the sensitivity and specificity of MRI exams, this reduction of inherent T1 contrast necessitates the development of efficient in vivo compatible positive contrast agent with good relaxivity characteristics at higher magnetic field5,6,7,8.

Protein cage nanoparticles, including ferritins, encapsulins and virus-like particles (VLPs), have been widely used as nanoscale delivery vehicles for diagnostic and/or therapeutic reagents9,10,11,12,13,14,15,16,17,18,19,20,21 as they not only have well-defined structure and size, but also consist of biocompatible and biodegradable biomaterials, polypeptides22. The highly symmetrical and uniformly composed architecture of protein cage nanoparticles allow them to be manipulated in a highly controlled manner, resulting in the production of reproducible nanoscale multifunctional particles9,10,11,12,13,14,15,16,17,18,19,20,21. VLPs have been extensively studied as supramolecular templates for the conjugation of small molecule contrast agents, such as the chelated paramagnetic gadolinium ion (Gd(III)), which is frequently used as a positive contrast agent for magnetic resonance imaging (MRI)23,24,25,26,27.

Macromolecule (such as protein)-based contrast agents are known to yield significantly higher r1 relaxivity due to decreased local motion and increased correlation time28,29,30; however in vivo applicable positive contrast agents, which maintain considerably enhanced r1 relaxivity (>10 mM−1s−1) with an acceptable r1/r2 ratio (0.5 ~ 1) at high magnetic field (7 T), are a rarity8,31,32,33,34.

In the present study, we used lumazine synthase, isolated from the hyperthermophile Aquifex aeolicus (AaLS), as a nanoscaled template for T1 contrast conjugates as illustrated in Fig. 1A and evaluated its potential as an in vivo MR contrast agent at the magnetic field strength of 7 T. AaLS is composed of 60 identical subunits that form an icosahedral capsid architecture (T = 1 state) with respective exterior and interior diameters of 15.4 nm and 9 nm35. AaLS is known to catalyze the penultimate step of riboflavin biosynthesis within cells36. The structural robustness and functional plasticity of AaLS make it an attractive candidate for a versatile bio- and nanotechnological platform37,38,39,40,41,42,43,44. Its hollow spherical architectures have been used as templates for the encapsulation of functional proteins37,43,44,45,46 and the biomimetic synthesis of iron oxides44, as well as delivery vehicles of therapeutic and/or diagnostic reagents32,34. AaLS has also been engineered into multifunctional building blocks for fabricating uniform layer-by-layer (LbL) assemblies using non-covalent interactions between surface-displayed hexahistidine and Ni-NTA of AaLS39. For our application, Gd(III)-chelating agent complexes were attached at position 108 with cysteine (R108C), which is known to be exposed on the exterior surface of AaLS.

Figure 1
figure1

(A) Surface and ribbon diagram representations of AaLS-RC (PDB: 1HQK) and site-specific conjugations of Gd(III)-DOTA (red dots) to the exterior surface of AaLS-R108C. (B) Molecular mass measurements of dissociated subunits of untreated AaLS-R108C (bottom) and Gd(III)-DOTA-AaLS-R108C (top). Calculated and observed molecular masses are indicated. (C) Size exclusion profiles of untreated AaLS-R108C (bottom) and Gd(III)-DOTA-AaLS-R108C (top). (D) Transmission electron microscopic image of negatively stained untreated AaLS-R108C (right) and Gd(III)-DOTA-AaLS-R108C (left) with 2% uranyl acetate.

Experimentally, Gd(III)-DOTA-AaLS were characterized by mass spectroscopy (MS) to ensure that AaLS was modified with only one Gd(III)-DOTA complex per subunit. Transmission emission microscopy (TEM) was used subsequently to ensure that the cage architecture and stoichiometry of AaLS is not significantly altered in the Gd complex conjugation. Next, r1 and r2 MR relaxivities were measured at 1.4 T and 7 T with multiple Gd concentration phantoms. In vivo imaging experiments were performed at the magnetic field strength of 7 T using a tumor bearing mouse, before and after the injection of Gd(III)-DOTA-AaLS-R108C. Observed signal behaviors were directly compared with the corresponding results from the injection of conventional Gd-DOTA (DOTAREM) for both vasculature and tumor regions.

Materials and Methods

Gd(III)-DOTA-AaLS-R108C as MRI contrast agent (MRI CA)

AaLS does not have sufficiently large inter-subunit spaces for Gd(III)-chelating agent complexes to freely diffuse into its interior cavity. Thus, to attach Gd(III)-chelating agent complexes in a site-specific and number controlled manner, we substituted the arginine residue at position 108 with cysteine (R108C), which is known to be exposed on the exterior surface of AaLS and readily to be used as a site for the attachment of small molecules47,48,49. To fully saturate the chelating agents (maleimido-monoamide-DOTA (DOTA-mal)) with Gd(III), they were incubated with an excess of Gd(III) prior to conjugation to AaLS. Subsequently, AaLS was treated with 3 mol equivalents of Gd(III)-DOTA-mal for attachment at room temperature with vigorous shaking overnight (Fig. 1A). Unreacted Gd(III)-DOTA-mal were removed by extensive dialysis with multiple changes of buffer. The extent of the conjugation was determined by subunit mass measurements using electrospray ionization time-of-flight mass spectrometry (ESI-TOF; Xevo G2 TOF).

Cell and animal models

Squamous cell carcinoma (SCC)-7 cells were cultured in RPMI1640 medium containing 10% (v/v) fetal bovine serum and 1% (w/v) penicillin-streptomycin at 37 °C under conditions of 5% CO2. To generate a tumor-bearing mice model, SCC-7 (1 × 106) cells in phosphate-buffered saline (PBS) were injected subcutaneously into the right flank of six-week-old female BALB/c nude mice weighing 20–25 g (Harlan Laboratories). When the tumor volume reached approximately 100 mm3, the mice were used for in vivo MR imaging. All animal studies were performed in compliance with the guidelines of the local ethics committee for animal care and use and were approved by the Institutional Animal Care and Use Committee of Ulsan National Institute of Science and Technology.

Relaxivity Measurements

We measured the T1 and T2 relaxation times of the Gd(III)-DOTA-AaLS under 1.4 T (Bruker Minispec MQ60 TD-NMR, 60 MHz) and 7 T (Bruker BioSpec 70/16 US, 300 MHz) magnets. At 60 MHz, the T1 relaxation times of the Gd(III)-DOTA-AaLS were measured using the inversion recovery (IR) method with the IR delay ranging from 0–20000 ms. T2 relaxation times were measured with a CPMG sequence with echo spacing (TE) of 1 ms and recycling time (TR) of 1.5 s. At 300 MHz, the T1 relaxation time was estimated using a sequence of rapid acquisition with relaxation enhancement (RARE) at variable repetition times; 10 TR values of 20–5000 ms were used with echo time (TE) of 7.6 ms. The T2 relaxation time was measured using a multi-slice multi-echo (MSME) method at TR of 5000 ms with 50 TE values in the range of 20–1000 ms. The proton relaxivities (r1 : longitudinal relaxivity and r2 : transverse relaxivity) were determined by measuring the T1 relaxation times and T2 relaxation times of six different Gd concentrations of Gd(III)-DOTA-AaLS-R108C (0.074, 0.037, 0.0185, 0.00925, 0.004625 and 0 mM). Both relaxivities r1 and r2 were calculated from the slope of the relaxation rate R1 (1/T1) and R2 (1/T2) as a function of concentration (mM) of contrast agent, respectively. We previously demonstrated that the relaxivities of protein cage nanoparticles conjugated with Gd(III)-chelating agent can be determined by measuring the T1 relaxation time and corresponding Gd(III) concentrations23. The relaxivities of Gd(III)-DOTA-AaLS-R108C were calculated using the linear relaxivity equation used in a previous study23,26. The proton relaxivities of saline solutions with untreated AaLS-R108C at identical concentrations were determined, as AaLS may also give T1 enhancement as well.

In vivo imaging of tumor-bearing mice

All tumor-bearing mice weighing 20–25 g (tumor volume: ~100 mm3) were scanned using 7 T MRI (Bruker Biospec) with a transceiver RF volume coil (diameter: 40 mm) for the mouse body, maintained under anesthesia with 1.0–1.5% isoflurane. Radiofrequency power and receiver adjustments were continuously maintained for each scan. Four mice were examined to investigate the vasculature imaging capabilities and the tumor accumulation efficacy of Gd(III)-DOTA-AaLS-R108C at high field (7 T) and two mice in the control group were assessed using Gd-DOTA (DOTAREM) as a reference. Four mice were injected intravenously with 350 μl of Gd(III)-DOTA-AaLS-R108C (concentration: 17.143 mM) as a bolus into the tail vein. Also in the same manner, two mice were injected intravenously with 350 μl of DOTAREM (concentration: 17.857 mM) as a bolus into the tail vein for the direct comparisons.

For the longitudinal and direct comparison of the vasculature imaging capability and delivery efficacy of Gd(III)-DOTA-AaLS-R108C to the tumor region, three dimensional (3D) MR angiography (MRA) and T1-mapping were adopted at six time points (1, 2, 3, 7, 12 and 30 h) after the intravenous injection of the contrast agent at a dose of 0.3 mmol/kg. To this end, a 3D-Fast Low-Angle Shot (FLASH) and a RARE with variable repetition time TR (RAREVTR) sequence were used for a high-resolution angiogram and T1-map, respectively. MR parameters were set as follows: TR = 13 ms, TE = 2.1 ms, flip angle = 20°, FOV = 30 × 30 × 30 mm3, matrix size = 256 × 256 × 256 for 3D-FLASH; TR = 8 values in the range of 280–5000 ms, TE = 6.0 ms, field of view (FOV) = 30 × 30 mm2, slice thickness = 1 mm, matrix size = 128 × 128 for RAREVTR T1 measurements. MRA were reconstructed using maximum intensity projection (MIP) protocol with Bruker Paravision software (PV6) and T1-maps were generated by a mono-exponential fitting method (Matlab, R2013a, The MathWorks Inc., USA).

Results

Characterization of Gd(III)-DOTA-AaLS-R108C

The extent of the Gd(III)-DOTA-conjugation was determined by ESI-TOF MS, following the dissociation of Gd(III)-DOTA-mal treated AaLS-R108C. The subunit molecular masses of AaLS-R108C and Gd(III)-DOTA-mal treated AaLS-R108C were determined to be 27415.0 and 28095.0 Da, respectively, which are in an excellent agreement with the corresponding calculated values of 27413.6 and 28096.9 Da and only one mass peak was observed (Fig. 1B). Mass spectrometric data indicated that all subunits of AaLS-R108C were modified with only one Gd(III)-DOTA complex (60 Gd(III)-DOTA per cage). The Gd-content of Gd(III)−DOTA conjugated AaLS-R108C was independently measured with inductively coupled plasma-mass spectrometry (ICP-MS) and the Gd-content was determined to be 0.86 Gd(III) per subunit, which is slightly lower than the value determined by MS probably due to slight sample loss during ICP-MS sample preparation23. To avoid overestimation of the relaxivity of Gd(III)-DOTA–conjugated AaLS-R108C (Gd(III)-DOTA-AaLS-R108C), we used the Gd content as determined by MS23. Gd(III)-DOTA-AaLS-R108C was eluted at the same position as untreated AaLS-R108C in size exclusion chromatography (Fig. 1C) and transmission electron microscopic images of negatively stained Gd(III)-DOTA-AaLS-R108C showed its intact cage architecture with the same size as untreated AaLS (~15 nm in diameter, Fig. 1D). Zeta potential measurements revealed that Gd(III)-DOTA-AaLS-R108C had slightly lower negative surface charge (−8.2 mV) than that of AaLS-R108C (−5.5 mV) probably due to the introduced Gd(III)-DOTA. These results demonstrate that Gd(III)-DOTA-mal conjugation to AaLS-R108C does not significantly alter its cage architecture or stoichiometry.

Relaxivity measurements with phantoms

The T1 relaxation times became shorter under both 1.4 T and 7 T, as the Gd(III) concentrations increased (Fig. 2A-1) suggesting that the Gd(III)-DOTA-AaLS-R108C supramolecular template accelerated the recovery of net magnetization. Corresponding T2 relaxation times also decreased as Gd(III) concentrations increased (Fig. 2A-2). Gd(III)-DOTA-AaLS-R108C showed higher T1 relaxivity (r1 = 30.24 mM−1s−1, r2 = 41.42 mM−1s−1, Fig. 2A-3) per Gd(III) under 1.4 T (37 °C) than did free Gd(III)-chelating agents (4–5 mM−1s−1). Although the T1 relaxivity of Gd(III)-DOTA-AaLS-R108C decreased (r1 = 16.49 mM−1s−1, r2 = 31.86 mM−1s−1, Fig. 2C-3) under high magnetic field (7 T, room temp.), the measured r1/r2 values of Gd(III)-DOTA-AaLS-R108C were 0.73 and 0.52 for 1.4 T and 7 T, respectively, indicating that Gd(III)-DOTA-AaLS-R108C remains as the optimal T1 contrast agent at 7 T23,26. The T1 and T2 relaxation times measurements with increasing concentration of untreated AaLS-R108C at identical experimental conditions were shown in Fig. 2B-1 for 1.4 T. Corresponding relaxations times measurements were shown in Fig. 2D-1 for 7 T. The relaxivities of untreated AaLS-R108C at 1.4 T (r1 = 0.37 mM−1s−1, r2 = 1.29 mM−1s−1, Fig. 2B-3) and 7 T (r1 = 1.02 mM−1s−1, r2 = 3.14 mM−1s−1, Fig. 2D-3) were determined as reference.

Figure 2
figure2

Measurements of T1 and T2 relaxation times of Gd(III)-DOTA-AaLS-R108C at 1.4 T (A-1,A-2) and 7 T (C-1,C-2).

The inverse values of the T1 and T2 relaxation times (R1 and R2) are plotted against Gd(III) concentration, whose slopes determine corresponding relaxivities (r1 and r2) for 1.4 T (A-3) and 7 T (C-3). Corresponding relaxation times and relaxivities were measured for untreated AaLS solutions and shown for 1.4 T (B-1B-3) and 7 T (D-1D-3).

To evaluate the potential usage of Gd(III)-DOTA-AaLS-R108C as an in vivo T1 contrast agent at high magnetic field, we captured T1-weighted MR images of the phantom using a volume coil with a 7 TMRI scanner using a RAREVTR sequence at 4 TR values (100, 500, 1000 and 2000 ms). T1-weighted in vitro phantom images of PBS control (S1A), AaLS-R108C only (S1B) and Gd(III)-DOTA-AaLS-R108C with increasing concentrations (S1C) were shown in supporting Fig. 1. While the MR signal intensities of Gd(III)-DOTA-AaLS-R108C were significantly enhanced at short TRs (500 and 1000 ms), those of the PBS control and AaLS-R108C were not, as shown in supporting Figure S1C, S1A and S1B, respectively. As the Gd concentration of Gd(III)-DOTA-AaLS-R108C is increased, the accelerated R1 values of Gd(III)-DOTA-AaLS-R108C led to contrast enhancement resulting in brighter images at short TRs as shown in Figure S1C. For the comparisons with conventional DOTAREM, saturation recovery signals with RAREVTR at multiple TR values were plotted together, where the Gd concentrations (0.0185, 0.037 and 0.074 mM) were kept same for both Gd(III)-DOTA-AaLS-R108C and DOTAREM as shown in S1D. Increased R1 values were apparent for Gd(III)-DOTA-AaLS-R108C at the same concentration, which led to enhanced r1 relaxivity over conventional DOTAREM at 7 T.

In vivo characterizations

3D MR angiograms and T1 maps of tumor-bearing mice were generated to evaluate both the feasibility of MR angiography and the tumor accumulation efficacy using newly developed Gd(III)-DOTA-AaLS-R108C. Identical experiments and analysis were repeated with conventional DOTAREM for direct comparisons. From these MR data, region of interest (ROI) analysis was also employed to measure regionally averaged T1 values and corresponding signal enhancement.

The decreasing trend in T1 values of the tumor region of mice was consistently observed after the time point of ~7 h post-Gd(III)-DOTA-AaLS-R108C injection. Figure S2A and S2B illustrates this, showing T1 maps before and 30 h after the injection. Supplementary Figure S2C shows the corresponding T1 fitting results at both time points, respectively. Decreased T1 values in the tumor region could clearly be observed 30 h after the injection of Gd(III)-DOTA-AaLS-R108C both with T1 maps and corresponding raw T1 fitting results.

MIP images of tumor bearing mice taken at sequential time points (pre, post, 2 h, 7 h, 12 h and 30 h) after the injection of Gd(III)-DOTA-AaLS-R108C were plotted in the first column of Fig. 3A. Immediately after the injection, blood vessels were brightened as the T1 values in the vessels significantly decreased due to the contrast agent injection. As time progressed, positive contrast in blood vessel diminished and ~7 h after the injection, the positive contrast in the tumor region started to appear. T1 shortening of tumor regions was further confirmed by T1 maps shown in the second column of Fig. 3B and the median-shift of the T1 histogram distribution of the whole 3D tumor volume, shown in the third column of Fig. 3C. Identical analysis was performed with conventional DOTAREM as shown in Fig. 3D–F. Blood vessels were also brightened right after the injection and dimming. Positive T1 contrast in the tumor region was apparent up to 2 h after the injection of DOTAREM and positive T1 contrast diminished and the T1 values were restored to original values 7 h post-injection. Longitudinal maximum intensity projection (MIP) images before and after the injections of Gd(III)-DOTA-AaLS-R108C (n = 4) and DOTAREM (n = 2) were shown in supporting Figure S3.

Figure 3
figure3

Representative (A) 3D MIP images, (B) T1-maps and (C) Histogram of T1 values at various time points after injection of Gd(III)-DOTA-AaLS-R108C. Representative (D) 3D MIP images, (E) T1-maps and (F) Histogram of T1 values at various time points after injection of conventional Gd-DOTA.

Figure 4A,B present ROI analysis, showing the temporal trend of signal enhancement measured in the arterial region after injection with Gd(III)-DOTA-AaLS-R108C and conventional DOTAREM. The signal enhancement trend in artery caused by both agents had a similar pattern. Directly after injection, maximal arterial signal enhancements were obtained from both contrast agents, this enhancement diminished as time progressed. A slower decreasing slope of arterial signal enhancement with Gd(III)-DOTA-AaLS-R108C than that from conventional DOTAREM was observed presumably from the elongated intravascular residence time of Gd(III)-DOTA-AaLS-R108C due to its size and hydrophilic surface. No meaningful arterial signal enhancements were observed 7 h and 30 h after injection with DOTAREM and Gd(III)-DOTA-AaLS-R108C, respectively.

Figure 4
figure4

Temporal signal enhancement in arterial region after injection of (A) Gd(III)-DOTA-AaLS-R108C and (B) Gd-DOTA. (C,D) Temporal variation of median T1 values in tumor and muscle regions after the injection of Gd(III)-DOTA-AaLS-R108C and Gd-DOTA, respectively. (* represents statistical significance with p < 0.05).

Temporal variations of T1 values in both tumor and muscle on the other side of flank after injection with Gd(III)-DOTA-AaLS-R108C and DOTAREM were presented in Fig. 4C,D. For Gd(III)-DOTA-AaLS-R108C, the T1 value in the tumor slowly decreased in the first 2 h post-injection and started to show a statistically significant change from 7 h post-injection (p < 0.05), while T1 in muscle showed no significant change at any time point. For DOTAREM on the other hand, the T1 value in the tumor reached the minimum value at the initial time point right after the injection and then rapidly rebounded and returned to its original level at 7 h. T1 in muscle showed a similar trend to T1 in tumor, with relatively reduced changes of T1 values at each time point.

Conclusions and Discussions

The high T1 relaxivity of Gd(III)-DOTA-AaLS-R108C may result from the slow tumbling rate of its large cage architecture28. Most previous studies performed with VLPs and protein complexes as supramolecular conjugates haven shown relaxivities of 10–50 mM−1s−1, depending on experimental conditions23,24,25,26,27. When compared with experiments of Gd(III)-DOTA chelating agent conjugates we have performed previously, our measured value at 1.4 T (30.24 mM−1s−1) was slightly lower than that of large VLPs, P22 K118C WB (35.8 mM−1s−1), but slightly higher than small sized ferritin (19.9 mM−1s−1)23. The T1 enhancement ability (r1/r2) tends to decrease significantly as the magnetic field is increased and often represents a major problem when applying macromolecular contrast agents at high field. The measured r1/r2 ratio (0.52) of Gd(III)-DOTA-AaLS-R108C at high magnetic field makes it attractive as a T1 contrast agent at 7 T. It appears that the molecular hindrance mechanism and resulting dynamics of Gd(III)-chelating agent complexes conjugated to lumazine synthase determine overall relaxation characteristics, but the exact explanation is unresolved at this point. Future relaxation simulation may shed more light on these experimental observations.

In vivo MIP images after the injection of Gd(III)-DOTA-AaLS-R108C show clearly defined vasculature, as shown in Fig. 3A. Compared with Gd-DOTA, which exhibited fast leakage in a rodent model of vasculature imaging, intravascular Gd(III)-DOTA-AaLS-R108C appears to provide robust MR angiography at 7 T. The temporal trend of signal enhancement in the arterial region shows that optimal signal enhancement of the vasculature is achieved within 2 h of intravenous injection of Gd(III)-DOTA-AaLS-R108C.

As the longitudinal relaxation rate (R1) in tumor is generally known to be proportional to Gd(III) concentration, the absolute T1 (1/R1) map of tumor and muscle regions on the other hind leg enables the monitoring of temporal variations in Gd(III) accumulation of both regions, with minimized animal repositioning and slice mismatch errors occurring in longitudinal follow up studies. In other words, decreasing absolute T1 values in tissue should reflect increasing Gd(III) concentration of corresponding region. The T1 value of the tumor decreased after the injected Gd(III)-DOTA-AaLS-R108C gradually migrated to the tumor region. A statistically significant decline in T1 value was observed 7 h post-injection, which coincides with a significant drop in signal enhancement in the arterial region. Compared to the short retention time (<2 h) of DOTAREM, due to its relatively small size and low molecular weight45, Gd(III)-DOTA-AaLS-R108C showed a prolonged retention time in tumor. A consistent T1 value in the other hind leg muscle away from the tumor supported the tumor-specific migration of Gd(III)-DOTA-AaLS-R108C, presumably due to the enhanced permeability and retention effects of the tumor46,51. These observations could be considered as the results of the abnormal characteristics of tumor tissue. In general, it is known that tumor tissues exhibit leaky vasculature and ineffective lymphatic drainage due to rapid and defective angiogenesis52. Hence, the tumor vasculature easily permits adequately sized macromolecules in plasma to escape from the tumor vessels and accumulate in tumor tissue for a specific time51. Meanwhile, although the long retention time of Gd(III)-DOTA-AaLS-R108C in tumor could be a strength in applications relating to anti-tumor therapy or drug delivery, it may raise toxicity concerns due to the release of free Gd(III) from prolonged retention and further in vivo toxicity study should be followed.

This study demonstrates that newly developed Gd(III)-DOTA-AaLS-R108C could be successfully applied as a positive T1 MR contrast agent at high field and utilized as a high-resolution vasculature imaging agent, active within 2 h of injection. Its prolonged retention time in tumors may be a key advantage for a potential theranostic nanoplatform, as well as for future scientific investigations of optimized MRI T1 contrast agent at higher magnetic field.

Additional Information

How to cite this article: Song, Y.K. et al. Lumazine Synthase Protein Nanoparticle-Gd(III)-DOTA Conjugate as a T1 contrast agent for high-field MRI. Sci. Rep. 5, 15656; doi: 10.1038/srep15656 (2015).

References

  1. McRobbie, D. W., Moore, E. A., Graves, M. J. & Prince, M. R. In MRI from Picture to Proton, Ch. 11, 206, Cambridge university press (2006).

  2. Yang, Q. X., Smith, M. B. & Wang, J. In Ultra High Field Magnetic Resonance Imaging, Ch. 9, 249–284, Springer US (2006).

  3. Salvolini, U. & Scarabino, T. In High Field Brain MRI, Ch. 4, 21, Springer-Verlag Berlin Heidelberg (2006).

  4. Yuh, W. T. et al. Clinical magnetic resonance imaging of brain tumors at ultrahigh field: a state-of-the-art review. Top. Magn. Reson. Imaging 17, 53–61. (2006).

    Article  Google Scholar 

  5. De Sousa, P. L. et al. In vivo MRI assessment of a novel GdIII-based contrast agent designed for high magnetic field applications. Contrast Media Mol. Imaging 3, 78–85 (2008).

    Article  Google Scholar 

  6. Caravan, P., Farrar, C. T., Frullano, L. & Uppal, R. Influence of molecular parameters and increasing magnetic field strength on relaxivity of gadolinium- and manganese-based T1 contrast agents. Contrast Media Mol. Imaging 4, 89–100 (2009).

    CAS  Article  Google Scholar 

  7. Helm, L. Optimization of gadolinium-based MRI contrast agents for high magnetic-field applications. Future medicinal chemistry, 2, 385–396 (2010).

    CAS  Article  Google Scholar 

  8. Hagberg, G. E. & Scheffler, K. Effect of r1 and r2 relaxivity of gadolinium-based contrast agents on the T1-weighted MR signal at increasing magnetic field strength. Contrast Media Mol. Imaging 8, 456–465 (2013).

    CAS  Article  Google Scholar 

  9. Aime, S., Frullano, L. & Geninatti Crich S. Compartmentalization of a Gadolinium Complex in the Apoferritin Cavity: A Route To Obtain High Relaxivity Contrast Agents for Magnetic Resonance Imaging. Angew. Chem., Int. Ed. 41, 1017–1019 (2002).

    CAS  Article  Google Scholar 

  10. Banerjee, D., Liu, A. P., Voss, N. R., Schmid, S. L. & Finn, M. G. Multivalent Display and Receptor-Mediated Endocytosis of Transferrin on Virus-Like Particles. ChemBioChem 11, 1273–1279 (2010).

    CAS  Article  Google Scholar 

  11. Destito, G., Yeh, R., Rae, C. S., Finn, M. G., & Manchester, M. Folic Acid-Mediated Targeting of Cowpea Mosaic Virus Particles to Tumor Cells. Chem. Biol. 14, 1152–1162 (2007).

    CAS  Article  Google Scholar 

  12. Han, J. A. et al. Ferritin protein cage nanoparticles as versatile antigen delivery nanoplatforms for dendritic cell (DC)-based vaccine development. Nanomedicine 10, 561–569 (2014).

    CAS  Article  Google Scholar 

  13. Kang, H. J. et al. Developing an antibody-binding protein cage as a molecular recognition drug modular nanoplatform. Biomaterials 33, 5423–5430 (2012).

    CAS  Article  Google Scholar 

  14. Kang, Y. J. et al. Polyvalent Display of Monosaccharides on Ferritin Protein Cage Nanoparticles for the Recognition and Binding of Cell-Surface Lectins. Macromol. Biosci. 14, 619–625 (2014).

    CAS  Article  Google Scholar 

  15. Kwon, C. et al. Development of Protein-Cage-Based Delivery Nanoplatforms by Polyvalently Displaying β-Cyclodextrins on the Surface of Ferritins Through Copper(I)-Catalyzed Azide/Alkyne Cycloaddition. Macromol. Biosci. 12, 1452–1458 (2012).

    CAS  Article  Google Scholar 

  16. Min, J. et al. Development of P22 Viral Capsid Nanocomposites as Anti-Cancer Drug, Bortezomib (BTZ), Delivery Nanoplatforms. Macromol. Biosci. 14, 557–564 (2014).

    CAS  Article  Google Scholar 

  17. Moon, H., Lee, J., Min, J. & Kang, S. Developing Genetically Engineered Encapsulin Protein Cage Nanoparticles as a Targeted Delivery Nanoplatform. Biomacromolecules 15, 3794–3801 (2014).

    CAS  Article  Google Scholar 

  18. Stephanopoulos, N., Tong, G. J., Hsiao, S. C. & Francis, M. B. Dual-Surface Modified Virus Capsids for Targeted Delivery of Photodynamic Agents to Cancer Cells. ACS Nano. 4, 6014–6020 (2010).

    CAS  Article  Google Scholar 

  19. Uchida, M. et al. Targeting of Cancer Cells with Ferrimagnetic Ferritin Cage Nanoparticles. J. Am. Chem. Soc. 128, 16626–16633 (2006).

    CAS  Article  Google Scholar 

  20. Zeng, Q. et al. Cucumber mosaic virus as drug delivery vehicle for doxorubicin. Biomaterials 34, 4632–4642 (2013).

    CAS  Article  Google Scholar 

  21. Liu, Q., Chen, S., Chen, J. & Du, J. An Asymmetrical Polymer Vesicle Strategy for Significantly Improving T1 MRI Sensitivity and Cancer-Targeted Drug Delivery. Macromolecules, 48, 739–749 (2015).

    CAS  ADS  Article  Google Scholar 

  22. MaHam, A., Tang, Z., Wu, H., Wang, J. & Lin, Y. Protein-Based Nanomedicine Platforms for Drug Delivery. Small 5, 1706–1721 (2009).

    CAS  Article  Google Scholar 

  23. Min, J. et al. Implementation of P22 Viral Capsids As Intravascular Magnetic Resonance T1 Contrast Conjugates via Site-Selective Attachment of Gd(III)-Chelating Agents. Biomacromolecules 14, 2332–2339 (2013).

    CAS  Article  Google Scholar 

  24. Garimella, P. D., Datta, A., Romanini, D. W., Raymond, K. N. & Francis, M. B. Multivalent, High-Relaxivity MRI Contrast Agents Using Rigid Cysteine-Reactive Gadolinium Complexes. J. Am. Chem. Soc. 133, 14704–14709 (2011).

    CAS  Article  Google Scholar 

  25. Lucon, J. et al. Use of the interior cavity of the P22 capsid for site-specific initiation of atom-transfer radical polymerization with high-density cargo loading. Nat. Chem. 4, 781–788 (2012).

    CAS  Article  Google Scholar 

  26. Qazi, S. et al. P22 Viral Capsids as Nanocomposite High-Relaxivity MRI Contrast Agents. Mol. Pharm. 10, 11–17 (2012).

    Article  Google Scholar 

  27. Pokorski, J. K., Breitenkamp, K., Liepold, L. O., Qazi, S. & Finn, M. G. Functional Virus-Based Polymer–Protein Nanoparticles by Atom Transfer Radical Polymerization. J. Am. Chem. Soc. 133, 9242–9245 (2011).

    CAS  Article  Google Scholar 

  28. Caravan, P., Ellison, J. J., McMurry, T. J. & Lauffer, R. B. Gadolinium(III) Chelates as MRI Contrast Agents: Structure, Dynamics and Applications. Chem. Rev. 99, 2293–2352 (1999).

    CAS  Article  Google Scholar 

  29. Caravan, P. Protein-Targeted Gadolinium-Based Magnetic Resonance Imaging (MRI) Contrast Agents: Design and Mechanism of Action. Acc. Chem. Res. 42, 851–862 (2009).

    CAS  Article  Google Scholar 

  30. Xue, S. et al. Design of ProCAs (protein-based Gd(3+) MRI contrast agents) with high dose efficiency and capability for molecular imaging of cancer biomarkers. Med. Res. Rev. 34, 1070–1099 (2014).

    CAS  Article  Google Scholar 

  31. Yang, J. J. et al. Rational Design of Protein-Based MRI Contrast Agents. J. Am. Chem. Soc. 130, 9260–9267 (2008).

    CAS  Article  Google Scholar 

  32. Li, S. et al. PEGylation of protein-based MRI contrast agents improves relaxivities and biocompatibilities. J. Inorg. Biochem. 107, 111–118 (2012).

    CAS  Article  Google Scholar 

  33. Kuda-Wedagedara, A. N. & Allen, M. J. Enhancing magnetic resonance imaging with contrast agents for ultra-high field strengths. Analyst 139, 4401–4410 (2014).

    CAS  ADS  Article  Google Scholar 

  34. Usselman, R. J. et al. Gadolinium-Loaded Viral Capsids as Magnetic Resonance Imaging Contrast Agents. Appl. Magn. Reson. 46, 349–355 (2015).

    CAS  Article  Google Scholar 

  35. Zhang, X., Meining, W., Fischer, M., Bacher, A. & Ladenstein, R. X-ray structure analysis and crystallographic refinement of lumazine synthase from the hyperthermophile Aquifex aeolicus at 1.6 Å resolution: determinants of thermostability revealed from structural comparisons. J. Mol. Biol. 306, 1099–1114 (2001).

    CAS  Article  Google Scholar 

  36. Zhang, X. et al. A Structure-based Model of the Reaction Catalyzed by Lumazine Synthase from Aquifex aeolicus. J. Mol. Biol. 328, 167–182 (2003).

    CAS  Article  Google Scholar 

  37. Beck, T., Tetter, S., Künzle, M. & Hilvert, D. Construction of Matryoshka-Type Structures from Supercharged Protein Nanocages. Angew. Chem., Int. Ed. 54, 937–940 (2015).

    CAS  Article  Google Scholar 

  38. Min, J., Kim, S., Lee, J. & Kang, S. Lumazine synthase protein cage nanoparticles as modular delivery platforms for targeted drug delivery. RSC Advances 4, 48596–48600 (2014).

    CAS  Article  Google Scholar 

  39. Moon, H. et al. Fabrication of uniform layer-by-layer assemblies with complementary protein cage nanobuilding blocks via simple His-tag/metal recognition. J. Mater. Chem. B 1, 4504–4510 (2013).

    CAS  Article  Google Scholar 

  40. Ra, J. S., Shin, H. H., Kang, S. & Do, Y. Lumazine synthase protein cage nanoparticles as antigen delivery nanoplatforms for dendritic cell-based vaccine development. Clin. Exp. Vaccine Res. 3, 227–234 (2014).

    CAS  Article  Google Scholar 

  41. Seebeck, F. P., Woycechowsky, K. J., Zhuang, W., Rabe, J. P. & Hilvert, D. A Simple Tagging System for Protein Encapsulation. J. Am. Chem. Soc. 128, 4516–4517 (2006).

    CAS  Article  Google Scholar 

  42. Wörsdörfer, B., Pianowski, Z. & Hilvert, D. Efficient in Vitro Encapsulation of Protein Cargo by an Engineered Protein Container. J. Am. Chem. Soc. 134, 909–911 (2012).

    Article  Google Scholar 

  43. Wörsdörfer, B., Woycechowsky, K. J. & Hilvert, D. Directed Evolution of a Protein Container. Science 331, 589–592 (2011).

    ADS  Article  Google Scholar 

  44. Shenton, W., Mann, S., Cölfen, H., Bacher, A. & Fischer, M. Synthesis of Nanophase Iron Oxide in Lumazine Synthase Capsids. Angew. Chem., Int. Ed. 40, 442–445 (2001).

    CAS  Article  Google Scholar 

  45. Bellin, M. F. & Van Der Molen, A. J. Extracellular gadolinium-based contrast media: an overview. Eur. J. Radiol. 66, 160–167 (2008).

    Article  Google Scholar 

  46. Goldmann, E. The Growth of Malignant Disease in Man and the Lower Animals, with Special Reference to the Vascular System. Proc. R. Soc. Med. 1, 1–13 (1908).

    CAS  PubMed  PubMed Central  Google Scholar 

  47. Su, H. et al. Rigid Mn (II) chelate as efficient MRI contrast agent for vascular imaging. Dalton Trans. 41, 14480–14483 (2012).

    CAS  Article  Google Scholar 

  48. Wang, L. et al. A multiple gadolinium complex decorated fullerene as a highly sensitive T1 contrast agent. Chem. Commun. 51, 4390–4393 (2015).

    CAS  Article  Google Scholar 

  49. Zhou, Z. et al. Surface and Interfacial Engineering of Iron Oxide Nanoplates for Highly Efficient Magnetic Resonance Angiography. ACS nano. 9, 3012–3022 (2015).

    CAS  ADS  Article  Google Scholar 

  50. Taurin, S., Nehoff, H. & Greish, K. Anticancer nanomedicine and tumor vascular permeability; Where is the missing link? J. Control. Release 164, 265–275 (2012).

    CAS  Article  Google Scholar 

  51. Peer, D. et al. Nanocarriers as an emerging platform for cancer therapy. Nat. Nanotechnol. 2, 751–760 (2007).

    CAS  ADS  Article  Google Scholar 

  52. Seymour, L. W. et al. Influence of molecular weight on passive tumor accumulation of a soluble macromolecular drug carrier. Eur. J. Cancer 31, 766–770 (1995).

    Article  Google Scholar 

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Acknowledgements

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (2010-0028684, 2013R1A1A1008228 and 2014R1A1A1008255).

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Y.K.S. and H.S.J. performed M.R. phantom and in vivo experiments. Y.J.K. and H.S.K. prepared contrast agent and did analysis. S.K. and H.C. conceived and designed experiments. All authors wrote the manuscript together.

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Song, Y., Kang, Y., Jung, H. et al. Lumazine Synthase Protein Nanoparticle-Gd(III)-DOTA Conjugate as a T1 contrast agent for high-field MRI. Sci Rep 5, 15656 (2015). https://doi.org/10.1038/srep15656

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