Although autosomal dominant polycystic kidney disease (ADPKD) is a common genetic disease and is characterized by the formation of multiple fluid-filled cysts, which results in renal failure, early diagnosis and treatment of ADPKD have yet to be defined. Herein, we observed that the promoter region of the gene encoding mucin-like protocadherin (MUPCDH) was hypermethylated in the renal tissue of patients with ADPKD compared to non-ADPKD controls. Inversely, MUPCDH was significantly repressed in ADPKD, especially in cyst-lining cells. Our results indicate that aberrant methylation of MUPCDH promoter CpG islands may be negatively correlated with reduced expression level of MUPCDH and that this contributes to abnormal cell proliferation in ADPKD. It suggests that methylation status of MUPCDH promoter can be used as a novel epigenetic biomarker and a therapeutic target in ADPKD.
Autosomal dominant polycystic kidney disease (ADPKD) is the most common inherited systemic disorder, with an incidence of 1 in 500–1,000 individuals1. ADPKD is characterized by the development of numerous fluid-filled cysts in both kidneys and this eventually leads to end-stage renal failure2,3. In particular, increased proliferation of renal tubular epithelial cells is considered as a major factor driving cyst formation and expansion in ADPKD4,5,6. ADPKD is caused by mutations in the PKD1 and PKD2 genes, which encode polycystin−1 and −2, respectively7. However, despite major causative genes being identified, the mechanism of cyst formation remains unclear and there are no effective therapeutic targets for ADPKD. Currently, the diagnosis of ADPKD is routinely based on the detection of kidney cysts via ultrasound imaging, which has limited sensitivity in patients with a mild disease phenotype, such as children, young adults and those with PKD2 mutations, even if they have a family history of ADPKD8. Moreover, as the glomerular filtration rate (GFR) remains stable for many decades in the early disease stage, even with the formation of multiple cysts9, the severity and progression of the disease cannot be easily evaluated and patients may not seek counseling for treatment.
Mucin-like protocadherin (MUPCDH), also known as mucin and cadherin-like protein, μ-protocadherin and cadherin-related family member 5, is a novel member of the cadherin superfamily10. The MUPCDH gene, containing 15 exons, is located on chromosome 11p15.5; it encodes a mucin-like 110-kDa type I transmembrane protein11. Classical cadherins contain five extracellular cadherin repeats and a conserved cytoplasmic region that interacts with the actin cytoskeleton via catenins. Functionally, the MUPCDH gene product is reported to be involved in calcium-dependent cell-cell adhesion, morphogenesis, cytoskeletal organization and cell migration12,13. Although it has been reported that downregulation of MUPCDH expression is a common event in colorectal cancer14, its function in colorectal cancer is not established at present. Furthermore, the MUPCDH protein is expressed in various epithelial tissues and especially at the apical surface of differentiated proximal tubule epithelial cells of the kidney, where it may have additional roles unrelated to adhesion11. However, thus far, the precise functions of MUPCDH in the kidney are unknown.
Recently, environmental factors such as epigenetic alteration, as well as genetic modifiers, have been suggested to affect the pathogenesis of ADPKD15. It seems that environmental changes affect the epigenetic status in the body and ultimately influence key signaling pathways that drive disease progression. In particular, DNA methylation is the most widely reported—and a stable—epigenetic marker16 that can heritably change gene expression without altering the DNA sequence17. In normal human cells, DNA methylation occurs at cytosine residues within CpG dinucleotides, which are mainly located in CpG islands (within the promoters of ~60% of genes) and is catalyzed by DNA methyltransferases (DNMTs). The establishment of aberrant DNA methylation patterns is associated with abnormal expression of certain genes, ultimately leading to diverse pathologies, such as cancer18. Interestingly, it appears that epigenetic modifications such as DNA methylation are early and tissue-specific events in tumorigenesis19 and therefore we could predict their application as potential prognostic markers in ADPKD, for renal cyst development, altered renal function and ultimately disease progression. Several studies have in fact investigated DNA methylation markers in urine sediments from patients with bladder cancer and reported that these epigenetic markers can be used for disease diagnosis20,21,22,23. In addition, epigenetic silencing is a potentially reversible alteration, which can be restored to the normal status by epigenetic therapy, unlike genetic mutation24.
In this study, we found that hypermethylation of MUPCDH promoter induces the transcriptional repression and increased cell proliferation in ADPKD. We suggested that urine methylation level of MUPCDH promoter region can be used as the novel epigenetic biomarker for prognosis of ADPKD.
Aberrant hypermethylation of the MUPCDH gene promoter region in ADPKD
In order to evaluate the DNA methylation pattern of genes related to cyst formation in ADPKD, methylated CpG island recovery assay-DNA sequencing (MIRA-seq.) analysis was carried out. The majority of genes, including MUPCDH, showed hypermethylation in their promoter region, which showed a negative correlation with the level of transcription (Supplementary Fig. S1a). The CpG island within the 5′ untranslated region (UTR) of the MUPCDH gene was significantly methylated in renal tissue from patients with ADPKD compared with non-ADPKD controls (Supplementary Fig. S1b). The DNA methylation pattern of the MUPCDH gene promoter region, which extends upstream from the translation start ATG for 2 kb to intron 1, was validated using methylation-sensitive high resolution melting (MS-HRM) analysis in individual clinical tissue samples and in renal cystic epithelial cells. Because of this analysis, the differentially methylated region (DMR) was identified as extending 1 kb upstream from the ATG of the MUPCDH gene (Supplementary Fig. S2). Following on from the MS-HRM analysis, the quantitative methylation status of the MUPCDH promoter was further confirmed using the EpiTYPER® assay. As expected, the upstream 1 kb region of the MUPCDH promoter was hypermethylated in both ADPKD renal tissue and WT9-7 ADPKD cyst-lining epithelial cells compared with non-ADPKD renal tissue and human renal cortical epithelial (HRCE) cells (Fig. 1). The data suggest that significant hypermethylation of the MUPCDH promoter CpG island is specifically observed in ADPKD.
Downregulation of MUPCDH gene expression in ADPKD
The expression levels of the MUPCDH mRNA and MUPCDH protein were confirmed using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and western blot analysis, respectively; significant repression of MUPCDH gene expression levels was observed in both ADPKD renal tissues and WT9-7 cells, compared with non-ADPKD renal tissues and HRCE cells (Fig. 2a–d). Interestingly, while MUPCDH protein is normally expressed apically along the brush border of the proximal convoluted tubule, its expression was dramatically suppressed in cyst-lining epithelial cells within ADPKD renal tissue (Fig. 2e). The data indicate that downregulation of the MUPCDH gene may be crucial to cyst development in ADPKD.
Correlation between MUPCDH promoter hypermethylation and its transcriptional repression
In order to establish whether the restoration of the DNA methylation level affects the regulation of MUPCDH gene expression, WT9-7 cells were treated with the demethylating agent, 5-aza-2′-deoxycytidine (5-aza-dC). Following treatment of the cells with 5-aza-dC, the DNA methylation level of the MUPCDH promoter region was decreased (Fig. 3a). In particular, as shown in Fig. 3a, specific promoter region presented by green dotted box was more sensitive to 5-aza-dC, compared to those in other regions. The data indicate that this particular region may be more critical for the regulation of MUPCDH transcription in the presence of DNA methylation. Furthermore, the mRNA and protein levels expressed from the MUPCDH gene were restored by demethylation of the promoter region in ADPKD cystic epithelial cells (Fig. 3b,c). Although demethylation induced by 5-aza-dC in WT9-7 cells was mean 73% within the green dotted box region, which was not similar compared with those of HRCE cells (~26%), it was sufficient to restore suppressed MUPCDH expression level (Supplementary Fig. S3). This in vitro result implies that reduced methylation of the MUPCDH promoter CpG islands can restore its expression at both the mRNA and protein level in ADPKD.
Association between MUPCDH promoter methylation level in urine samples and renal progression in human ADPKD
To evaluate the potential as a prognostic biomarker of ADPKD, DNA methylation levels in the proximal promoter region of the MUPCDH gene were measured in random urine samples (n = 53) from patients with ADPKD using MS-HRM analysis. We classified the subjects into five groups according to methylation level of the MUPCDH promoter (Fig. 4a,b) and compared the total kidney volume (TKV) and estimated glomerular filtration rate (eGFR) among groups. The mean age of subjects was 49 ± 9.3 years and they were 53% male. Baseline TKV and eGFR were 1491 ± 632.5 ml and 60.8 ± 18.1 ml/min per 1.73 m2. The mean interval between the baseline and final value was 21 months. There was no significant difference in baseline TKV (Fig. 4c), nor the rate of annual change of TKV (Fig. 4d) according to MUPCDH promoter methylation level. However, when the subjects were grouped into 0–75% and 100% methylation groups, the percent annual change in TKV was significantly faster in the 100% methylation group than the 0–75% group (median 14.6% vs. 5.9% per year, P = 0.019 by Mann-Whitney test) (Fig. 4e). In contrast, no significant trend was observed in baseline kidney function or annual change in eGFR (Supplementary Fig. S4). This result implies that the fully methylated MUPCDH promoter may reflect the progression of cyst growth in ADPKD.
Transcription factor AP-2α involved in the promoter activity of the MUPCDH gene
To further investigate which specific region within the MUPCDH gene promoter is critical to the regulation of gene transcription, serial deletion constructs of the MUPCDH gene 5′ UTR were generated by cloning appropriate PCR-amplified fragments (Fig. 5a) into the pGL3-basic luciferase reporter vector and transfected into human embryonic kidney HEK293T cells. As shown in Fig. 5b, the pGL3-299 and pGL3-571 constructs showed higher luciferase activity than the pGL3-148 construct, but this was only a 1.5-fold enhancement compared with the pGL3-19 construct. Importantly, the promoter activity of pGL3-19 was sufficient to activate expression of the reporter gene (Fig. 5b), indicating that this region of the promoter has a critical role in transcriptional regulation of the MUPCDH gene. Promoter analysis using programs such as those in the TRANSFAC® database indicated that the consensus sequence of transcription factor AP-2α is located at −14 to −2 in the MUPCDH gene promoter (Fig. 5a). To verify that the AP-2α binding site is necessary to promote MUPCDH transcription, the predicted consensus sequence for AP-2α binding within the pGL3-19 construct was mutated by site-directed mutagenesis and the effect of this mutation on promoter activity was measured in the luciferase assay. Mutation of the AP-2α binding element significantly reduced the expression of the reporter (Fig. 5c). These data support the conclusion that pGL3-19 construct may be required for the regulation of MUPCDH promoter activity. Moreover, the results of a chromatin immunoprecipitation-qRT-PCR (ChIP-qPCR) assay for AP-2α binding showed significant enrichment of RNA polymerase II and AP-2α on the MUPCDH proximal promoter sequence in HRCE cells which have a high level of MUPCDH gene expression (Fig. 6a). Whereas, other predicted possible transcription factors such as Sp1, WT1, Stat5 and HLTF, did not show significant enrichment even though their binding sites are overlapped each other within the proximal promoter region of MUPCDH (Supplementary Fig. S5), supporting the specificity of the AP-2α into the MUPCDH promoter. Transfection of AP-2α-targeted short interfering RNA (siRNA) induced a reduction in the level of MUPCDH expression (Fig. 6b,c). To further confirm the interaction between AP-2α and the MUPCDH gene, the luciferase assay was carried out following transfection of the AP-2α siRNA into HEK293T cells. Upon knockdown of AP-2α, MUPCDH promoter activity was reduced to a much lower level in pGL3-19 construct (Fig. 6d). However, interestingly, overexpression of AP-2α in WT9-7 cells could not significantly increase the level of MUPCDH gene expression (Supplementary Fig. S6a). In contrast, this was not the case in normal renal epithelial cells, as the level of MUPCDH expression was increased upon overexpression of AP-2α in HRCE cells (Supplementary Fig. S6b). These data suggest that overexpressed AP-2α protein can bind to its binding element in the hypomethylated MUPCDH promoter in HRCE cells but not in WT9-7 cells, where the MUPCDH promoter is hypermethylated. Therefore, the data suggest that the activity of the MUPCDH promoter may be regulated by DNA methylation, as well as by the interaction of AP-2α with the promoter.
Regulation of transcription factor AP-2α binding affinity by an altered DNA methylation pattern in the MUPCDH promoter
An increased level of DNA methylation in the proximal promoter region of the MUPCDH gene may be a key factor in the regulation of MUPCDH gene transcription. To investigate methylation-associated inactivation of the MUPCDH gene in ADPKD, serial deletion constructs for use in the luciferase assay were treated with SssI methylase in vitro and then transfected into HEK293T cells; increased methylation of promoter constructs induced significant repression of overall promoter activity in comparison with untreated constructs (Fig. 7a). In particular, hypermethylation of the proximal promoter region of the MUPCDH gene (corresponding to construct pGL3-19) inhibited promoter activity, similarly to the mutant pGL3-19 construct (Fig. 7b). Moreover, the enrichment of RNA polymerase II and AP-2α were increased by treatment of WT9-7 cells with 5-aza-dC (Fig. 7c). Intriguingly, the enrichment of active histone modification marks such as acetyl-histone H3 (acetyl-H3) and trimethyl histone H3 (Lys4) (H3K4 me3) were increased, while the inactive trimethyl histone H3 (Lys9) (H3K9 me3) mark was decreased, after treatment of WT9-7 cells with 5-aza-dC (Fig. 7d). These data indicate that the methylation pattern of the proximal promoter region of the MUPCDH gene may affect the binding affinity of AP-2α and the transcription level associated with histone modification marks.
Inhibition of cell proliferation by restoration of the level of MUPCDH expression
To investigate how silencing of the MUPCDH gene affects the progression of cyst formation due to elevated cell proliferation in ADPKD, knockdown or overexpression of the MUPCDH gene was induced in HRCE and WT9-7 cells. When the MUPCDH gene was downregulated in HRCE cells, cell proliferation was increased (Fig. 8a). In contrast, overexpression of MUPCDH in WT9-7 cells induced the suppression of cell growth (Fig. 8b). To further confirm the anti-proliferative effect of MUPCDH, the 5-bromo-2′-deoxyuridine (BrdU) incorporation assay was carried out following transfection of MUPCDH-targeted siRNA into HRCE cells. As expected, the incidence of BrdU-positive cells was greater in cells transfected with the MUPCDH siRNA than in control siRNA-transfected cells (Fig. 8c). In addition, overexpression of the MUPCDH gene significantly reduced both the level of proliferating cell nuclear antigen (PCNA) mRNA and the number of PCNA-positive cells (Fig. 8d). Intriguingly, treatment with 5-aza-dC induced the inhibition of cell proliferation, in agreement with the data for MUPCDH overexpression (Fig. 8e). Taken together, these results indicate that the MUPCDH gene may be involved in abnormal proliferation of renal epithelial cells and that restoration of DNA methylation can regulate the anti-proliferative property of MUPCDH.
Hypermethylation is a common event in the silencing of tumor suppressor genes in most cancer types25. Although ADPKD pathogenesis is also associated with epigenetic alterations such as histone modification and dysregulation of micro RNAs26,27, epigenetic biomarkers for the diagnosis or prognosis of ADPKD have not yet been established. In our previous study, we suggested the potential role of epigenomic alteration, especially DNA methylation, in cystogenesis of ADPKD, which is a genetic disorder15. In this study, we determined that a novel protocadherin gene associated with cyst growth in ADPKD is regulated by DNA methylation of its promoter region. We also examined the relationship between hypermethylation of the MUPCDH promoter and the clinical phenotypes of ADPKD patients. Intriguingly, in urine samples of patients with ADPKD, the fully methylated MUPCDH promoter was associated with faster kidney volume progression, suggesting that it may be a valuable prognostic biomarker of cyst growth in ADPKD. In particular, MS-HRM analysis is a simple and useful technique for simultaneously detecting DNA methylation patterns at multiple CpG sites using a small quantity of genomic DNA and for various samples. However, our analysis had the following limitations: 1) the sample size was relatively small, especially that of the 100% methylation group; 2) the average interval between the baseline and final value was 21 months years; and 3) MUPCDH is mainly expressed in proximal tubular cells, whereas cysts in ADPKD originate from both proximal and distal tubules, explaining a weak correlation with disease progression. Independent sets of serial samples with longitudinal data, including total kidney volume and renal function, are required to establish the validity of MUPCDH as a reliable ADPKD biomarker.
As reported in several previous studies, MUPCDH is a novel member of the cadherin superfamily that includes E-cadherin and may play a role in calcium-dependent cell adhesion28. However, as MUPCDH is enriched at the apical surface of renal proximal tubule epithelial cells11, it may have additional functions that affect renal physiology and pathology. Our results suggest a novel function of MUPCDH, i.e., its anti-proliferative effect in the kidney. The loss of this function is important for the process of cyst formation, consistent with the significantly repressed level of MUPCDH expression across the cyst-lining epithelial cells in ADPKD renal tissue samples.
In a previous study, the MUPCDH gene was shown to be a target of the Cdx2 transcription factor in the colon cancer29. However, it is possible that another transcription factor is also required for the regulation of MUPCDH transcription, because the expression of MUPCDH and Cdx2 did not completely overlap in colon cancer cells and the transcriptional regulation of MUPCDH expression by Cdx2 depended on the cell line29. We identified another transcription factor, AP-2α, that is specifically associated with the regulation of MUPCDH transcription. AP-2α has well-known roles in cell growth, normal morphogenesis and apoptosis in mammalian cells30,31. A recent study also revealed that the AP-2α protein is overexpressed in nasal polyp epithelia compared to normal nasal mucosa and promotes expression of the MUC8 gene, which is an important mucin gene and the major component of mucus32. Moreover, AP-2α binds to CpG islands in the promoter regions of various genes and is involved in gene transcription33.
More intriguingly, our data showed that transcription of the MUPCDH gene and the functions of its gene product at the cellular level are regulated by promoter methylation. Treatment of renal cyst-lining epithelial cells with the DNA demethylating agent, 5-aza-dC, induced the re-expression of the MUPCDH gene; these results are in agreement with previous studies showing that DNA methylation is responsible for gene silencing34. Additionally, our data suggested an explanation for the importance of differential patterns of DNA methylation in promoter regions for transcriptional regulation. DNA methylation may influence gene transcription via two major mechanisms. First, the association between the methylated DNA and methyl-CpG-binding domain (MBD) proteins recruits additional proteins to the locus, such as histone deacetylases and other chromatin remodeling proteins and ultimately leads to the alteration of the chromatin structure. Second, methylated cytosine residues interfere with the binding between transcription factors and their binding sites in the promoter region35. Because many transcription factor-binding sites do not include CpG dinucleotides, the second mechanism has been thought to have minimal transcriptional effects. However, we demonstrated that AP-2α is another transcriptional regulator of the MUPCDH gene. Although the relatively low efficiency of AP-2α transfection into WT9-7 cells compare with HRCE cells may be responsible for the lack of response in MUPCDH transcription level, it is also possible that the methylation of CpG sites in close proximity to the binding site changes the DNA double helix configuration or interferes with cofactor binding and indirectly inhibits AP-2α binding. To our knowledge, this is the first report to identify a critical regulatory region within the MUPCDH promoter and its high correlation with the DNA methylation pattern in the kidney. Further studies are needed to clarify the possible mechanism of the reduced binding affinity between the transcription factor and promoter sequence, including identification of cofactors that bind AP-2α.
Transcriptionally active promoters have a chromatin structure that is enriched for active histone marks such as acetyl-H3 and H3K4 me3 and enrichment of these active histone marks was induced following treatment of cyst-lining epithelial cells with the DNA demethylating agent, 5-aza-dC. In contrast, a reduction in H3K9 me3, which is an important modification for transcriptional repression, was observed in 5-aza-dC-treated cyst-lining epithelial cells. These data indicate that histone modification is involved in the aberrant DNA methylation pattern of the MUPCDH promoter region. However, it is still unclear how histone modification and DNA methylation cooperate to achieve gene silencing. It has been reported that the link between DNA methylation and histone modification might be partially mediated by MBD proteins, which recruit histone deacetylases to methylated DNA36. In addition, 5′-methylcytosine can also recruit dimethylated H3K9 (H3K9 me2), which is a repressive mark, by interacting with both histone methyltransferase G9a and DNMT1 and can interfere with H3K4 me3 interactions37,38. These interactions help explain how DNA methylation affects chromatin structure and gene silencing. Therefore, they should be validated in order to elucidate the relationship between DNA methylation and histone modification in the transcriptional regulation of MUPCDH.
In conclusion, our study shows that hypermethylation of a CpG island in the promoter region of the MUPCDH gene regulates its transcriptional level and affects the proliferative capacity of renal epithelial cells. These data imply that the differential methylation pattern of the MUPCDH promoter in non-ADPKD and ADPKD renal tissues is a potential target for a novel epigenetic therapeutic reagent, such as a demethylating agent, in ADPKD. Encouraged by these results, we plan to confirm that the methylation status obtained from urinary genomic DNA can actually affect cyst development and cause loss of renal function in ADPKD.
Renal tissue and urine samples from subjects with ADPKD
Renal cyst tissue was obtained from patients with ADPKD undergoing nephrectomy. As controls, non-ADPKD renal tissue specimens were obtained from patients undergoing surgery for clear cell renal cell carcinoma; malignant cell infiltration was excluded by histology. These tissues were all used in our previous study15. First or second, freshly voided urine samples were collected and stored at −70 °C from 53 subjects with ADPKD. Clinical information such as gender, age, eGFR and kidney volume was obtained. Estimated GFR was calculated by using MDRD formula based on serum creatinine traceable to isotope dilution mass spectrometry. All the subjects were ascertained to have mild to moderate renal insufficiency (chronic kidney disease stage 2 ~ 3 b). Computed tomography was performed every two year and the TKV was measured by modified ellipsoid method. Rate of change in eGFR and TKV was calculated by subtracting final value from baseline, then dividing by baseline value, resulting percent annual change (% per year). The study was approved by the Institutional Review Board of Seoul National University Hospital (H-0701–033–95 and H-0901–046–269). Written informed consent was obtained from all subjects in accordance with the Declaration of Helsinki.
Treatment of cell lines with 5-aza-dC and siRNA
HEK293T and WT9-7 cells39 were cultured in Dulbecco’s modified Eagle’s medium (Welgene, Dalseo-gu, Korea) supplemented with 10% (v/v) fetal bovine serum (FBS) and penicillin-streptomycin. For use in control experiments, HRCE cells were obtained from Clonetics (San Diego, CA, USA). HRCE cells were cultured in renal epithelial cell growth medium (Clonetics) with the following supplements: 0.5% FBS, human transferrin (10 mg/ml), hydrocortisone (0.5 mg/ml), insulin (5 mg/ml), triiodothyronine (5 × 10−12 M), epinephrine (0.5 mg/ml), epidermal growth factor (10 mg/ml) and penicillin-streptomycin. Cells were grown in a humidified atmosphere at 37 °C in 5% CO2 and 95% air. The cells were treated with 5 μM 5-aza-dC (Sigma-Aldrich, St Louis, MO, USA) for 72 h. The medium was changed daily thereafter and fresh 5-aza-dC added as before. For transient transfection with siRNA, siRNA duplex oligoribonucleotides targeting either MUPCDH or AP-2α were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). The siRNA was transfected into either HRCE or HEK293T cells using LipofectamineTM RNAiMAX (Invitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions. Subsequent experiments were performed 24–48 h after transfection.
Bisulfite treatment and DNA methylation analysis
Genomic DNA was isolated from human renal tissue and renal cells, before and after treatment with DNMT inhibitors, using the NucleoSpin® TriPrep Extract kit (MACHEREY-NAGEL, Düren, Germany), according to the manufacturer’s protocol. Genomic DNA was extracted from urine specimens of 1.75 ml using the Urine DNA Isolation Kit (Norgen Biotek Corp., Thorold, ON, Canada), according to the manufacturer’s protocol. The EZ DNA Methylation-GoldTM kit (Zymo Research, Orange, CA, USA) was used for bisulfite treatment of genomic DNA, according to the manufacturer’s instructions. DNA methylation patterns were validated in two steps, using MS-HRM and EpiTYPER® analyses. For MS-HRM analysis, primers covered the region extending for 2 kb from the translation start ATG upstream to intron 1 (Fig. 1 and Supplementary Table S1). PCR amplification was carried out in a total volume of 20 μl, containing the following: 10× Buffer, 1.65 mM MgCl2, 200 μM each of the four dNTPs, 500 nM each primer, 0.04 U HotStarTaq DNA polymerase (QIAGEN, Hilden, Germany), ResoLight dye (Roche, Branchburg, NJ, USA) and 10 ng of bisulfite-modified template. High-resolution melting was analyzed using the LightCycler® 96 Instrument (Roche). EpiTYPER® primers and the MUPCDH promoter region extending 1 kb upstream from the ATG are shown in Fig. 1 and Supplementary Table S2. For in vitro transcription, reverse primers were tagged with the bacteriophage T7 RNA polymerase promoter sequence and 10-mer sequences were added to the forward primer for balance. Bisulfite-treated genomic DNA was amplified by PCR as follows: 94 °C for 15 min; 45 cycles of 94 °C for 20 sec, 62 °C for 30 sec and 72 °C for 1 min; followed by 72 °C for 3 min. Unincorporated dNTPs left over following amplification were neutralized by incubation at 37 °C for 20 min with shrimp alkaline phosphatase (SAP) and inactivated at 85 °C for 5 min. In vitro RNA transcription, with an RNase A cleavage reaction cocktail containing 0.64× T7 polymerase buffer, 3.14 mM dithiothreitol, T7 R&DNA™ polymerase, 0.09 mg/ml RNase A, T- or C-cleavage mix and SAP-treated PCR product, generated transcripts that had undergone enzymatic base-specific cleavage. The molecular mass of fragments was determined by matrix-assisted laser desorption/ionization mass spectrometry and use of the EpiTYPERTM software (Sequenom, San Diego, CA, USA), which automatically generates a report that contains quantitative information for each fragment analyzed.
Total RNA extraction and real-time qRT-PCR
Total RNA was extracted using the NucleoSpin® TriPrep Extract kit (MACHEREY-NAGEL), according to the manufacturer’s protocol. RNA (2 μg, isolated as described above) was reverse-transcribed using M-MLV reverse transcriptase (Promega, Madison, WI, USA), 100 nM oligo-dT, 1 mM each of the four dNTPs and RNase inhibitor. The following primers were used for real-time qPCR: human MUPCDH (forward 5′-GCCGACCTTGCCCGCTACTCA-3′ and reverse 5′-CGCCACGGTGCCACGATACAG-3′), human Aquaporin 1 (AQP1) (forward 5′-TATGCGTGCTGGCTACTACCGA-3′ and reverse 5′-GGTTAATCCCACAGCCAGTGTAG-3′) and human ACTB that encodes β-actin (forward 5′-AAGGCCAACCGCGAGAAGAT-3′ and reverse 5′-CCAGAGGCGTACAGGGATAGCAC-3′). Real-time qPCR was carried out using the quantitative SYBR® kit (JMC R&D, Mansfield, QLD, Australia) in the Rotor-Gene 3000® (Corbett Robotics, San Francisco, CA, USA), as described in the manufacturer’s instructions. PCR cycling conditions were as follows: 15 min at 95 °C, then 40 cycles of 10 sec at 95 °C, 15 sec at 60 °C and 20 sec at 72 °C.
Western blot analysis
Equal amounts of protein were analyzed in duplicate by SDS-PAGE. The following monoclonal antibodies were used: anti-MUPCDH (anti-μ-protocadherin, C-20; Santa Cruz Biotechnology), anti-AP-2α (Santa Cruz Biotechnology), anti-AQP1 (Santa Cruz Biotechnology) and anti-β-actin (Bethyl Laboratories, Montgomery, TX, USA). Immunoreactive proteins were detected by horseradish peroxidase-conjugated secondary antibodies and enhanced chemiluminescence reagents (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA). All immunoblots were performed with triplicate and visualized by LAS image analyzer (Fujifilm, Tokyo, Japan). The band density was quantified using MultiGauge (Fujifilm).
Paraffin-embedded sections of renal tissue derived from patients with ADPKD and controls were melted in a 60 °C oven and deparaffinized in three changes of xylene, followed by rehydration in a graded ethanol series. Sections were heated at 100 °C for 15 min in 0.01 M citric acid (pH 6.0) for antigen retrieval and blocking was performed for 1 h at room temperature with blocking solution (VECTASTAIN® ABC kit, Vector Laboratories Inc. Burlingame, CA, USA), followed by overnight incubation at 4 °C with anti-MUPCDH primary antibody (anti-μ-protocadherin, C-20; Santa Cruz Biotechnology). Incubation with 4′,-6-diamidino-2-phenylindole was carried out in order to stain cell nuclei. Fluorescent signals were visualized using a fluorescence microscope (Olympus IX-81).
Serial deletion fragments of the MUPCDH promoter region (−571, −299, −148, −19/+1; +1 being the transcription start site) were amplified by PCR and cloned into a pGL3-basic luciferase vector (Promega). A bacterial artificial chromosome clone (RP11; Invitrogen) was used as a template for PCR. A NheI site was added to the 5′-primer and a XhoI site was added to the 3′-primer (these sites are underlined in the primer sequences). The sequences of the PCR primers used to generate serial deletion constructs were as follows: −571 forward 5′-ctagctagcGGGCCAATGAAAAGAGCA-3′, −299 forward 5′-ctagctagcGCCCCTGCCCTGCCCCTACC-3′, −148 forward 5′-ctagctagcGGGGTGGCCTTAGGAACTTTG-3′, −19 forward 5′-ctagctagcCTGCCCCTCCTTCTGGCAGTG-3′/+ 1 reverse 5′-ccgctcgagCTTGGCGGCTGTCACCTGGC-3′. The constructs were verified by sequencing of both DNA strands.
The prediction of transcription factor binding sites was carried out using the TRANSFAC®database and the promoter region of AP-2α was subjected to site-directed mutagenesis to identify the potential activator of the MUPCDH gene promoter. Site-directed mutagenesis was carried out using the QuikChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA), according to the manufacturer’s instructions. The mutated sites are underlined as follows in the sequence corresponding to the forward primer for the −19 serial deletion fragment: 5′-CTGCCCCTCCTTCTGGCAGTG-3′→ 5′-CTGCCCCTTGGTGTGGCAGTG-3′.
Transfection and luciferase reporter assays
For in vitro treatment with methylase SssI, cloned pGL3-constructs were redigested with the NheI and XhoI restriction enzymes and then purified using the ZymocleanTM Gel DNA Recovery Kit (Zymo Research). Purified insert fragments were subjected to in vitro methylation by methylase SssI in the presence of the methyl group donor, S-adenosylmethionine and monitored by digestion with the SmaI enzyme. Methylated insert fragments were subsequently purified using DNA Clean & ConcentratorTM-5 Kit (Zymo Research) and ligated with digested pGL3-basic vector. The ligation mixture was directly transfected into cells. For transfections, HEK293T cells were plated into 6-well plates at a density of 2 × 105 per well and luciferase constructs (2 μg of reporter gene plasmid and 50 ng of phRL-CMV normalizing plasmid) were transfected into the cells 1 day after seeding using FuGENE® HD transfection reagent (Roche), according to the manufacturer’s instructions. After transfection for 48 h, luciferase activity was measured by the Dual Luciferase Assay system (Promega) and relative luciferase activity was calculated by normalization to the Renilla luciferase activity of the phRL-CMV plasmid. All transfection experiments were carried out in duplicate and repeated at least three times.
ChIP was performed as previously described40, using antibodies specific for RNA polymerase II (ab817; Abcam, Cambridge, UK), AP-2α (sc-184; Santa Cruz Biotechnology), H3K4 me3 (17-614; EMD Millipore, Billerica, MA, USA), H3K9 me3 (ab8898; Abcam) and H3Ac (06–599; EMD Millipore). Sequences of the MUPCDH promoter primers were as follows: forward 5′-CCCTGCACTCAGTCCAACTT-3′ and reverse 5′-TTCC TACCTCAGCGACCTTC-3′.
Cell proliferation assays
To assess cell proliferation, WT9-7 and HRCE cells were seeded into 10-cm culture dishes at a density of 2.5 × 104 per dish and transfected with pCMV-SPORT6 (Open Biosystems, Huntsville, AL, USA) expressing MUPCDH, or MUPCDH-targeted siRNA, using FuGENE® HD transfection reagent (Roche) and LipofectamineTM RNAiMAX (Invitrogen), respectively. After 24 h, cells were plated at a density of 1,000 per well in 96-well plates and grown for 1, 2, 3 and 4 days in standard culture medium. The proliferation of each cell line was measured by a colorimetric assay using the WST-1 Cell Proliferation Assay Kit (Roche), according to the manufacturer’s instructions. Absorbance of the converted dye was measured at a wavelength of 470 nm. In the BrdU incorporation assay, transfected cells were incubated in culture medium containing 10 μMBrdU (Sigma-Aldrich) for 2 h and fixed with 4% v/v formaldehyde in phosphate-buffered saline (PBS). After washing with PBS, cells were treated with 2 M HCl for 20 min at 37 °C and neutralized with 0.1 M sodium borate buffer for 15 min at 37 °C. Cells were permeabilized with 0.1% Triton X-100 for 15 min and then washed with PBS. Finally, the cells were incubated with a mouse monoclonal anti-BrdU antibody (Sigma-Aldrich) at 4 °C overnight. For PCNA staining, transfected cells were fixed with 4% paraformaldehyde for 10 min at room temperature and then washed three times with PBS. Cells were permeabilized with 0.2% Triton X-100 containing 5% v/v bovine serum albumin and incubated with an anti-PCNA antibody (Santa Cruz Biotechnology) at 4 °C overnight. Immunofluorescence images for BrdU incorporation and PCNA staining were visualized using a confocal microscope (Olympus IX-70).
Each experiment was repeated at least three times throughout the study. Data were reported as the mean ± SD. Statistical significance was examined using Student’s t test when only two groups were compared, otherwise one-way analysis of variance with Kruskal-Wallis test or Mann-Whitney test as appropriate was carried out, using the GraphPad Prism 5.0 program (GraphPad Software, San Diego, CA, USA). P < 0.05 was considered as significant.
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This research was supported by grants from the Bio & Medical Technology Development Program of the NRF (2012M3A9D1054519 and 2010–0019867) and Basic Research Program (2013R1A2A1A01011908) of the NRF funded by the Korea government (MSIP). Professor HP Kim of Yonsei University, Korea helped with the ChIP assay technique. Human kidney tissues of RCC and ADPKD patients were obtained from Dr. Curie Ahn at Department of Nephrology in Seoul National University Hospital.
The authors declare no competing financial interests.
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Woo, Y., Shin, Y., Hwang, JA. et al. Epigenetic silencing of the MUPCDH gene as a possible prognostic biomarker for cyst growth in ADPKD. Sci Rep 5, 15238 (2015). https://doi.org/10.1038/srep15238
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