The generation of genetically-modified organisms has been revolutionized by the development of new genome editing technologies based on the use of gene-specific nucleases, such as meganucleases, ZFNs, TALENs and CRISPRs-Cas9 systems. The most rapid and cost-effective way to generate genetically-modified animals is by microinjection of the nucleic acids encoding gene-specific nucleases into zygotes. However, the efficiency of the procedure can still be improved. In this work we aim to increase the efficiency of CRISPRs-Cas9 and TALENs homology-directed repair by using TALENs and Cas9 proteins, instead of mRNA, microinjected into rat and mouse zygotes along with long or short donor DNAs. We observed that Cas9 protein was more efficient at homology-directed repair than mRNA, while TALEN protein was less efficient than mRNA at inducing homology-directed repair. Our results indicate that the use of Cas9 protein could represent a simple and practical methodological alternative to Cas9 mRNA in the generation of genetically-modified rats and mice as well as probably some other mammals.
Engineered nucleases, such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein 9 (Cas9) are key tools for the introduction of gene-inactivating mutations by non-homologous end joining (NHEJ) mechanisms, as well as precise modifications such as base mutation, exon exchange or insertion of expression cassettes by homology-directed recombination (HDR)1,2. Engineered nucleases injected into zygotes as nucleic acids have been particularly useful for the generation of genetically modified animals in several species in which it was previously impossible or very difficult due to the absence of robust ES cells2,3. This is particularly the case in the rat, an important animal model4,5 that has been successfully genetically modified to create new disease models4,6,7,8 as well as biotechnological applications9. The CRISPR/Cas9 system using Cas9 mRNA has been used to generate genetically-engineered rats10,11,12 and mice13,14. However, the HDR frequencies are low and increasing them would accelerate and facilitate the generation of genetically modified animals useful in the analysis of genes, creation of disease models and production of recombinant proteins1,2,5.
We reasoned that if the engineered nucleases could be delivered as proteins it would increase HDR efficiency since the transcription and/or translation steps would be avoided and degradation of the gene targeting construct limited, which would increase its availability. To the best of our knowledge, only one publication describes the use of Cas9 protein to obtain integration of short sequences, using ssODNs in zebrafish15, and two other publications gene invalidation by NHEJ, in zebrafish and mice16 and C. elegans17. Every other genetically engineered animal model, including rats and mice, using any type of nuclease, including meganucleases, ZFNs and TALENs, has been generated using DNA or mRNA2,3, TALEN proteins, in particular, have never been used in the generation of transgenic animals. In this context, we recently described HDR in the rat Rosa26 locus using TALEN mRNA and a large GFP expression cassette1. However, improving the targeted integration of long transgenes by HDR remains a challenge and the benefit of using nucleases directly as proteins has not been tested. In the present work, we generated and purified Cas9 protein and two TALEN monomers targeting the rat Rosa26 locus (for the TALEN proteins, they correspond to those expressed from mRNA used previously to obtain HDR). We microinjected into rat zygotes these engineered protein nucleases along with a long donor DNA fragment encoding for GFP, previously used to obtain HDR using TALEN encoded by mRNA1. Using Cas9 protein we observed higher efficiency in NHEJ and HDR compared to Cas9 mRNA. In contrast, TALEN proteins were less efficient than TALEN mRNA. We also targeted the mouse Rosa26 locus using Cas9 protein and mRNA and a long donor DNA fragment and also observed a higher efficacy of Cas9 protein at generating HDR. Cas9 protein was also found to be more efficient at generating HDR compared to Cas9 mRNA in two more rat loci targeted, Foxp3 and Anks3, using, respectively, a long donor DNA fragment and ssODNs.
In conclusion, our results suggest that in rats and mice the use of Cas9 protein may be more efficient than mRNA at inducing HDR using both large and short DNA donor fragments. The use of Cas9 protein may widen the application of genome-editing techniques in different species.
Generation of TALEN and Cas9 proteins and validation of their DNA cleavage in rat cells
The rat Rosa26 loci were targeted in intron 1. TALEN proteins targeted sequences that had already been mutated using mRNA and were generated in vitro from mRNA (Fig. 1A)1. Cas9 protein was generated in vitro and single guided RNA (sgRNA) targeting sequences in between those recognized by the TALENs at the rat Rosa26 locus were designed (Fig. 1A) and confirmed as functional by transfecting rat cells (Fig. 1B). The frequencies of indel generation (Fig. 1B) were in the range of efficiency obtained in other loci in our hands with this technology and were among the highest after testing several sgRNAs for this portion of the rat Rosa26 locus (data not shown). TALENs and Cas9 proteins (expected size of 160 kDa pour Cas9 and 110 kDa for TALENs) were purified and showed to be largely clear of contaminants (Fig. 1C). With both TALEN and Cas9 purified proteins we observed efficient cleavage of rat Rosa26 sequences in vitro (Fig. 1D).
Microinjection into rat zygotes of TALEN and Cas9 proteins or mRNA targeting the Rosa26 locus along with a long donor DNA fragment to obtain HDR
We then microinjected the TALEN or Cas9 proteins along with a large donor DNA fragment comprising a GFP cassette and homologous sequences previously used to obtain HDR with TALENs as mRNA1 (Fig. 2A). Microinjection of rat zygotes was into the pronuclei and the cytoplasm using a mixture of either a Cas9/sgRNA protein complex (at equimolar concentration for both of 0.3, 3 or 6 μM), Cas9 mRNA/sgRNA or TALEN proteins (at 4 or 10 μM for both monomers) along with a long donor DNA fragment (4.7 Kb, 2 ng/μl). The donor DNA contained homology arms of 0.8 Kb and a CAG-GFP expression cassette previously shown to induce HDR in the rat Rosa26 locus when used with TALENs mRNA1. The donor DNA was linearized before injection, since, as we have shown previously1, only linear, and not circular DNA, results in HDR. The use of this linear DNA resulted in the generation of transgenic rats by random integration but the circular form also generated transgenic rats at only slightly lower frequencies1. Day 15 fetuses were analyzed under UV light to detect GFP expression, and transgene integration was also confirmed by PCR amplification of the GFP coding sequence. HDR was determined by Southern blotting as well as by PCR using primers encompassing predicted integration sites followed by amplicons sequencing. NHEJ was analyzed by PCR of the targeted sequence followed by a T7 endonuclease I assay and sequencing of the amplicons.
Cas9 protein (complexed in vitro with sgRNA) injected at a concentration of 0.3 and 3 μM did not result in embryo toxicity but 6 μM resulted in a drastic reduction in the percentage of viable day 15 fetuses (Table 1). Cas9 protein at 0.3 μM resulted in neither HDR nor NHEJ mutations. Cas9 protein at 3 μM resulted in HDR in 1.7% and NHEJ in 11.1% of transferred eggs (i.e. 3.5% HDR and 22.8% NHEJ of day 15 embryos). At 6 μM, in spite of the marked toxicity, we observed some HDR (0.7%) and NHEJ (2.1%) (Table 1). Among fetuses microinjected at a concentration of 3 μM, two were confirmed as true HDR by positive junction PCRs (Fig. 2B) showing genomic sequences outside the homology arms (Fig. 2C) as well as bands of the expected size in Southern blots (Fig. 2D). Both fetuses positive for HDR showed GFP expression whereas the other fetuses were negative (Fig. 2E). In contrast, Cas9 mRNA and sgRNA failed to generate HDR and resulted in NHEJ in 2.35% of transferred eggs (or 7.55% of day 15 embryos) (Table 1). At 0.3 μM, where the Cas9 protein induced neither HDR nor NHEJ, several fetuses expressed GFP, but they contained randomly inserted DNA (Table 1).
Microinjection of TALEN proteins at a concentration of 8 and 20 μM TALENs generated NHEJ only in the higher concentration group, and there in 2.6% of microinjected embryos, and failed to generate HDR (Table 1). Precipitation of purified TALENs prevented microinjection at higher concentrations. Since TALEN mRNA (coding for the same TALENs) resulted in HDR in 2.2% and NHEJ in 3.3% of transferred eggs1, TALENs proteins in concentrations compatible with microinjection were less efficient than mRNA for both NHEJ and HDR.
NHEJ analysis of mutated founders showed an increased rate of mosaicism using Cas9 protein, since 42.1% of the fetuses were mosaics compared with 25% using Cas9 mRNA. It also tended to be higher using TALEN protein (33%) (data not shown) compared with TALEN mRNA (21.7%), as described in a previous work1.
These results show that for the rat Rosa26 locus Cas9 protein but not TALEN proteins were more efficacious to obtaining HDR than the respective mRNA and this was associated to a higher rate of NHEJ.
Microinjection into mouse zygotes of TALEN and Cas9 proteins or mRNA targeting the Rosa26 locus along with a long donor DNA fragment to obtain HDR
To extend the results obtained with Cas9 protein in rats to mice, we microinjected mouse zygotes using the same technique used for rats. The sgRNA targeted intron 1 in the mouse Rosa26 locus and the donor DNA used was a linear longer fragment (12.5 kb, 2 ng/μl) compared to the rat one, aimed at introducing an expression cassette for CAG-podocan coding sequences (Fig. 3A). Preliminary experiments showed that Cas9/sgRNA protein complex microinjected at 6 and 3 μM resulted in embryo mortality (data not shown) whereas microinjections at 0.3 or 1.5 μM resulted in acceptable embryo survival and day 13 fetus development comparable to that observed with Cas9 mRNA at 20 ng/μl (Table 2) and to those obtained with other constructs (data not shown). Day 13 fetuses that had been microinjected with Cas9 protein at 0.3 μM exhibited HDR in 2.85% of transferred embryos (16.7% of analyzed fetuses) and NHEJ in 7.1% of transferred embryos (42% of analyzed fetuses) (Table 2). Both fetuses (N° 47 and 64) obtained with Cas9 protein at 0.3 μM showed PCR positive expression cassette, PCR positive for 3′ integration but not for the 5′ integration PCR (Fig. 3B) and Southern blot bands of expected size using a 3′ probe but not a 5′ probe (Fig. 3C), and thus represent incomplete HDR. Cas9 protein microinjected at 1.5 μM resulted in HDR in 1.5% of transferred eggs (16.7% of analyzed fetuses) and NHEJ in 9.1% of transferred eggs (100% of analyzed fetuses). In one fetus (N° 28) HDR was evidenced by positive expression cassette and flanking PCRs of the expected size (Fig. 3B) and Southern blot with 5′ and 3′ bands of the expected size (Fig. 3C). Fetuses that were microinjected with Cas9 mRNA/sgRNA resulted in no HDR and in NHEJ in 13.4% of transferred eggs (94% of analyzed fetus) (Table 2). One fetus showed positive podocan PCRs but not flanking PCRs and thus represents a random genomic insertion of the donor DNA (Table 2). Thus, for the mouse Rosa26 locus Cas9 protein was more efficient in generating HDR than Cas9 mRNA, whereas for NHEJ induced mutations Cas9 protein and mRNA were comparable.
Microinjection of Cas9 proteins or mRNA targeting the Foxp3 locus into rat zygotes to obtain HDR
To extend and confirm the results obtained in the Rosa26 locus, we targeted the rat Foxp3 locus using a sgRNA for a sequence of the last gene exon (exon 11) situated just 5′ of the stop codon (Fig. 3A). Rat zygotes were microinjected in the pronuclei and the cytoplasm using a mixture of either a Cas9/sgRNA protein complex (equimolar 3 μM) or mRNA/sgRNA along with donor DNA. The donor DNA contained a 5′ homology arm of 1 Kb comprising Foxp3 exons 9–11 and the respective introns, a T2A-GFP-stop codon sequence and a 3′ homology of 1 Kb arm comprising Foxp3 3′ untranslated sequences (Fig. 4A). Day 15 embryo DNA was analyzed by PCR of the GFP coding sequence while GFP expression was not analyzed since it is under the control of the Foxp3 promoter and it is not expected at this early time point in ontogeny. HDR and NHEJ were analyzed by PCR and sequencing of the amplicons of 5′ and 3′ insertion extremities of the donor DNA and of the exon 11 targeted sequence, respectively. Cas9 protein was not toxic and resulted in a HDR rate of 0.8% of transferred eggs (3.44% of day 15 embryos, n = 29) and in a NHEJ rate of 1.6% of transferred eggs (6.9% of day 15 embryos) (Table 3 and Fig. 4B). In contrast, Cas9 mRNA did not result in the generation of HDR and resulted in NHEJ in 2.6% of transferred eggs (7.7% of day 15 embryos analyzed n = 52) (Table 3 and Fig. 4B). For both Cas9 protein and mRNA, animals with random insertion of the donor DNA were identified with similar frequencies (0.8% and 0.7%, respectively) (Table 3). Sequencing of the amplicons generated with primers flanking the 5′ and 3′ insertion extremities showed the exact wild type sequences as well as internal T2A and GFP indicating faithful HDR (Fig. 4C). There were no mosaic animals among any of the Cas9 protein or mRNA mutated animals (data not shown).
Thus, for the Foxp3 locus also, Cas9 protein was more efficient than Cas9 mRNA at generating HDR using a long DNA donor fragment with NHEJ rates comparable.
Microinjection of Cas9 proteins or mRNA targeting the Anks3 locus along with ssODNs into rat zygotes to obtain HDR
Since for the Rosa26 loci the increase in HDR using Cas9 protein could be the result of the associated increase of NHEJ that was also observed, we decided to decrease the concentration of sgRNA to suboptimal conditions for DNA cleavage and analyze HDR efficiency under these conditions. For this, we used a sgRNA for exon 10 of the rat Anks3 gene (Fig. 5A) that showed high NHEJ activity with Cas9 mRNA and we used the Cas9/sgRNA protein complex at the optimal Cas9 protein concentration and a lower concentration of the sgRNA (3 μM/1.5 μM) compared with the previous two loci. For the Anks3 locus, the objective was to introduce a point mutation (ATC to GAA or I35E) and for this we microinjected ssODNs (7.5 ng/μl) instead of long DNA donor fragments. The donor DNA also contained silent mutations that created an EcoRI site to facilitate the detection of HDR. As far as we know, the use of Cas9 protein and ssODNs has not been used before in mammals since the only previous publication looked at zebra fish15.
Pups born after microinjection were analyzed by PCR of exon 10 encompassing the whole donor sequence and extending into the genomic unmodified sequence (Table 4 and Fig. 5B). Animals generated with Cas9 protein showed HDR in 4 animals, 2.6% of transferred zygotes (18.2% of born animals). In 2 out of these 4 animals both alleles were HDR as evidenced by PCRs with bands of the expected size that were cleaved by EcoRI (Table 4 and Fig. 5B) and by sequencing of these PCR products (Fig. 5C and data not shown). All of these HDR were complete, with no indels in the extremities (Fig. 5C and data not shown). In contrast, only one animal generated with Cas9 mRNA (N° 3.3) showed HDR and this was not faithful since indels were observed in both extremities of the inserted sequence but that they did not affect the EcoRI site (Table 4, Fig. 5C and data not shown). The rate of NHEJ was higher in animals generated using Cas9 mRNA since 6.7% of transferred zygotes (41.2% of born animals) displayed NHEJ mutations whereas animals generated with Cas9 protein (n = 22) showed 2.6% NHEJ in transferred zygotes (18.2% of born animals) (Table 3). Mosaicism was observed in 66.5% of Cas9 protein and 25% of Cas9 mRNA injected animals. Thus, Cas9 protein was also more efficient at generating HDR and resulted in higher degree of mosaicism than Cas9 mRNA for a locus in which NHEJ was high and in favor of Cas9 mRNA.
The generation of genetically-modified animals has been greatly improved recently by the direct microinjected in one-cell stage embryos of gene-specific nucleases, such as ZFNs, TALENs and CRISPRs-Cas92,3,5. This technique has made it possible to generate targeted knockout and knockin genes in species other than mice, i.e. species which do not have the advantage of the existence of robust ES cells. In these species, the generation of genetically modified animals was based on inefficient, cumbersome techniques, such as random mutagenesis, random transgene integration by DNA microinjection and cloning by somatic nuclear transfer. Even in the mouse, for which there are robust ES cells-based technologies, genome editing is now being increasingly carried out using gene-specific nucleases since they represent an advantage in terms of both time and cost2. Despite these advances, the efficiency in the generation of genetically-modified animals, particularly knockin, using gene-specific nucleases, is still low. Therefore, it would seem advantageous to increase the efficiency of DNA cleavage and/or donor DNA integration.
The use of protein nucleases could increase the rate of DNA cleavage and/or of HDR efficacy since they can cleave the DNA faster than when delivered as mRNA and could thus increase the availability of donor DNA for incorporation into the cleavage point. Cas9 protein microinjected in zebrafish embryos induces NHEJ faster compared to mRNA16, whether this can be confirmed in mammal zygotes still needs to be analyzed. Cas9 protein shows increased HDR of short sequences using ssODNs in zebrafish compared to mRNA15. Two other studies have used Cas9 protein for gene invalidation by NHEJ (no HDR reported), they show comparable mutation rates to mRNA in zebrafish and mice16 but there was no comparison to mRNA in C. elegans17. This present work applies Cas9 protein to rats for the first time and in addition, obtains HDR of a large DNA donor fragment in rats and mice. This was not explained by higher frequencies of NHEJ since we observed that with Cas9 protein or Cas9 mRNA using the same sgRNA these frequencies varied; in the rat Rosa26 locus Cas9 protein was more efficient than Cas9 mRNA, but in the mouse Rosa26 locus, as well as in the rat Foxp3, there was comparable efficiency, and for the Anks3 locus the NHEJ frequencies for Cas9 protein were lower than those for Cas9 mRNA. Thus, the differences in the concentration of sgRNA used when Cas9 protein or mRNA were microinjected (higher for the former) cannot explain the observed differences in NHEJ. As for HDR, Cas9 protein was more efficient than Cas9 mRNA in every case when using both long donor DNA fragments and ssODNs. In fact, several of the HDR-mutated rats and mice could not have been generated without the use of Cas9 protein. When using ssODNs, Cas9 mRNA did generate HDR, but showed indels in the extremities of the incorporated sequences and this introduced out of frame mutations when the objective was to introduce a single amino acid mutation. In contrast, all the HDRs obtained using Cas9 protein and ssODNs were without indels. The better HDR results obtained with Cas9 protein using both the long and short donor DNA suggest that faster HDR kinetics could avoid donor DNA degradation before genomic integration.
The concentration of the donor DNA microinjected (2 ng/μl) into the pronucleous and immediately after into the cytoplasm is the one that we have previously used as optimal when performing HDR using TALENs1,18. This concentrations is lower compared to those used in other studies using microinjection into the pronucleous of long double stranded DNA as donors in the rat [4 ng/μl10,11,12 or 10 ng/μl19], as well as in mice [10 ng/μl20] but in our technical conditions higher concentration of DNA results in excessively high embryo toxicity. Higher concentrations of long double stranded donor DNA were optimal when microinjected into the cytoplasm but the optimal for pronuclear microinjection were much lower (200 as compared to 10 ng/μl, respectively)14. The concentration of ssODNs we used were higher (7.5 ng/μl) but the differences in toxicity with long double stranded donor DNAs can be explained by intrinsic differences of both DNA forms.
The long donor DNAs were used in a linear form because we have shown that two different DNA donors, including the one for CAG-GFP for the rat Rosa26 locus, generated HDR when using TALENs only when in a linear form and not in a circular one1. Previous publications in mice and rats showed that in some of them both linear and circular DNA generated HDR21,22,23,24 whereas others showed that only the linear one was successful25. Furthermore, although the circular form has been described as reducing genome integration as compared to the linear form26, in our conditions, both the linear and circular forms generated a comparable proportion of random integration1.
Mutated animals generated using nucleases expressed by nucleic acids frequently exhibit mosaicism1,2, probably due to the initial action of nucleases after the one-cell stage embryo stage and/or the persistent action of nucleases beyond the one-cell zygote stage, which re-cleave alleles that were repaired faithfully by NHEJ at the one-cell stage. Thus, a potential advantage of the use of protein nucleases could be a lower percentage of mosaic animals, since cleavage could begin in the one-cell stage embryos. However, if the half-life of the protein goes beyond the one-cell stage embryo stage, then its action could be a source of mosaicism. The results in the present study show that some Cas9 protein obtained animals are mosaic and the frequency is comparable to that using Cas9 mRNA, suggesting that this is possibly due to the half-life of Cas9 and its action being beyond the one-cell embryo stage. Previous analysis of C. elegans mutants obtained using Cas9 protein showed the presence of mosaic animals and although this work did not include a comparison with the use of mRNA, it does show that mosaicism was not eliminated17. Mosaicism using Cas9 protein could eventually be reduced using a lower protein concentration, thus favoring its action at the one-cell embryo stage.
Although GFP expression in tissues of the rat Rosa26 HDR fetus harboring a CAG-GFP expression cassette was not analyzed, we have previously shown that this same cassette introduced by HDR in the rat Rosa26 locus using TALEN mRNA resulted in fetuses that are brightly and uniformly GFP positive and correlated with widespread tissue expression on adult animals1.
To the best of our knowledge this is the first time that TALEN proteins have been used to obtain genetically-modified animals. Despite TALEN proteins performing less well than mRNA, the highest concentration microinjected still maintained embryo survival and while using higher concentrations might have obtained higher mutation rates, this was technically not possible due to TALEN protein precipitation. We cannot exclude other TALEN purification techniques obtaining higher concentrations. Nevertheless, the ease of generation of sgRNAs and the universal function of Cas9 along with the superior results in transgenesis clearly point to the CRISPR-Cas9 system being more versatile than TALENs. At the same time, for some sequences for which TALENs are already available, such as the ones targeted here in the rat Rosa26 locus, the rate of embryos with NHEJ or HDR with the TALEN mRNA (3.3%)1 was comparable to that of the CRISPRs/Cas9 protein system (3.5% at 3 μM of Cas9 protein).
Although ZFNs27, TALENs28 and Cas929 fused to cell-penetrating peptides or anionic proteins30 have been delivered as proteins to cells in vitro, and resulted in comparable or superior efficacies to mRNA for NHEJ and HDR using ssODNs30, these techniques are not applicable to zygotes due to the zona pellucida barrier.
The rat is a widely used experimental model that is gaining popularity due to the generation of many new genetically engineered models using gene-specific nucleases5. The increase in efficiency and refinement of the genome editing and transgenic technologies through gene-specific nucleases should increase the use of rats as an alternative or complement to other experimental species.
In conclusion, we have shown that the use of Cas9 protein increased NHEJ and/or HDR efficiency depending on the locus when compared to injection of Cas9 coding mRNA. In contrast, injection of TALEN proteins was less efficient than injection of coding mRNA. Combined with the other numerous advantages of CRISPR/Cas9 technology in the generation of genetically-modified animals, the use of Cas9 protein further increases the efficiency and applications in genome editing.
The rats used in this work were from the Sprague-Dawley (SD/Crl) strain (Charles River, L’Arbresle, France). The mice were from the C57Bl6/N strain (Janvier Labs, Le Genest St Isle, France). The study was approved by the Ethics Committee on Animal Experimentation of the Pays de la Loire Region, France, in accordance with the guidelines from the French National Research Council for the Care and Use of Laboratory Animals (Permit Numbers: CEEA-PdL-2011-45). All efforts were made to minimize suffering.
Plasmid construction for TALEN expression in E.coli
TALE nucleases for rat Rosa26 locus (Mar. 2012 (RGSC 5.0/rn5) genome Assembly. ROSA26 (chr4: 208314007-208314058)).
recognize a sequence in intron 1 and have been already described1. These sequences were subcloned in the bacterial expression vector pSKB3 (kind gift from Dr. Stephen K Burley) using an In Fusion cloning kit (Clontech) (see sequences in Supplementary Materials). The pSKB3-ROSA constructs were expressed in the E. coli Rosetta (DE3) pLysS (Novagen) strain. One kanamycine resistant clone was inoculated in 50 mL of LB containing Kanamycine (50 μg/mL) and 2% glucose and was grown overnight at 37 °C at 200 rpm. Next morning, 2 L Erlenmeyer flasks containing 500 mL of LB supplemented with Kanamycin sulfate (50 μg/mL) and 1% glucose were inoculated at OD600 = 0, 1 and bacteria were grown at 37 °C, 200 rpm for about 3 h to reach OD600 = 1.2 to 1.4. IPTG was added to a final concentration of 0.2 mM and induction was done for 22 h at 16 °C, shaking to 200 rpm. Cells were harvested and washed with PBS before freezing at −80 °C. Cells were sonicated in 20 mL of lysis Buffer (25 mM Tris-OAc pH 8.0, 300 mM NaCl, Protease Inibitor Ultra -EDTA (Roche), 1 mM PMSF) and lysate was centrifugated at 92,600 g at 4 °C for 1h30 in a 70TI rotor (Beckman). Supernatant was loaded on 2 mL of HIS-select-HF Nickel affinity gel (SIGMA), extensively washed with 25 mM Tris-HCl pH 8.0, 300 mM NaCl followed by 8 mL of 25 mM Tris-OAc pH 8.0, 300 mM NaCl, 10 mM Imidazole and eluted in 8 mL of 25 mM Tris-OAc pH 8.0, 300 mM NaCl, 250 mM Imidazole. Proteins were precipitated with 20% ammonium sulfate (adding 0.85 g to 8 mL) and centrifuged 30 min at 4 °C at 13,000 g. Precipitated proteins were solubilized overnight on ice in 300 μL of 25 mM Tris-OAc pH 8.0, NaCl 300 mM, EDTA 1 mM, TCEP 1 mM, protease inhibitor complete (Roche). Protein was further purified on a Superdex 200 GL 10/300 (GE-Healthcare) using an AKTA purifier 10 against 25 mM Tris-OAc pH 8.0, 300 mM NaCl, 1 mM EDTA. Protein was concentrated to 21 μM on microcon 10 kDa (Millipore), supplemented with 10% glycerol and flash frozen in nitrogen before storage at −80 °C.
Plasmid construction for S. pyogenes Cas9-3NLS nuclease expression in E. coli
Plasmid pMJ806 was obtained from Addgene (http://www.addgene.org/39312) and since 2xNLS were more efficient at targeting Cas9 to the nucleus than 1xNLS31 we introduced 3xNLS by ligation independent cloning (Gibson Assembly, New England Biolabs) (see coding sequences in Supplementary Materials).
Expression and purification of S. pyogenes Cas9-3NLS nuclease
Expression and purification of S. pyogenes Cas9 protein was adapted from Jinek et al.32. The main modification consisted in carrying out protein expression using a 3 L computer-controlled bioreactor (Lambda, CZ) using growth conditions as described in Marisch et al.33. Briefly, 2, 5 mL of a pre-culture of Rosetta2 bacterial strain transformed with pMJ806-3NLS construct at OD600 = 0.8 was inoculated in a semi synthetic batch medium (1.5 L), containing for the present set up 4.5 g KH2PO4, 9 g K2HPO4.3H2O, 25 g of glucose.H2O, 2.1 g of sodium citrate (trisodium salt. 2H2O), 0.84 g MgSO4.7H2O, 0.168 g CaCl2.2H2O, 1.26 g of yeast extract, 420 μL of trace element solution prepared as described in Marisch et al.33, and 50 mg/L of kanamycin sulfate. The pH was maintained at 7.0 by addition of 14% NH4OH. Foaming was automatically suppressed by addition of Antifoam C emulsion (Sigma) as the modulation of conductance was detected with the foam formation (maximum 0.5 mL/L). Bacteria in batch medium were grown for 11 h at 37 °C with 5 Hz vertical stirring and 4 L/min air flow (DO600 = 3.5). Exponential feeding with a flow rate allowing addition of 600 mL of fed batch medium in 42 h according to exponential growth algorithm X = X0.eμt (with μ = 0.1 h−1) was then started overnight. Composition of the fed batch medium was the following: 3 g/L of KH2PO4, 6 g/L of K2HPO4.3H2O, 21 g/L of sodium citrate (trisodium salt. 2H2O), 9 g/L MgSO4.7H2O, 1.74 g/L of CaCl2.2H2O, 4.3 mL/L of trace element solution, 258 g/L of glucose. H2O and 50 mg/L of kanamycin sulfate. After 18 h of feeding, (OD600 = 16.2) temperature was decreased to 16 °C and protein expression was induced by a first pulse of IPTG (65 μM) followed by a second pulse 30 min later (+0.325 mM). 0.2 mM IPTG was also added to the feed medium. Feeding was continued for 23 h to reach 2 L with OD600 = 32 and 118 g of bacterial pellets. Protein purification was then carried out as described in Jinek et al.32.
Guide RNA plasmid construction
The sgRNA for rat Rosa26 targets a sequence (GTGTATGAAACTAATCTGTCTGG) (PAM in italics) in intron 1, in bold in the TALEN Rosa26 sequence depicted above. The sgRNA for mouse Rosa26 targets a sequence (GAAGATGGGCGGGAGTCTTCTGG) (PAM in italics) in intron 1. The sgRNA for rat Foxp3 targets a sequence (GCAGGGGTTGGAGCACTTGCTGG, PAM in italics) in 3′ end of the last exon (exon 11), just before the stop codon. The sgRNA for rat Anks3 targets a sequence (GGACGCATAGAAAGACTGGGAGT, PAM in italics) in exon 10.
sgRNAs were cloned in MLM3636 and DR274 derived vectors (http://www.addgene.org/43860/ and http://www.addgene.org/42250/) for U6 and T7 transcription and used in cell cultures or for one-cell embryo injections, respectively. For cellular experiments and mRNA injection, human codon-optimized Cas9-expression vector (hCas9, http://www.addgene.org/41815/) was used.
In vitro transcription and purification of mRNA
sgRNAs were transcribed using the DraI-digested sgRNA expression vectors DR274 as templates and the T7 high yield kit (New England Biolabs). Following transcription DNase I (New England Biolabs) treatment was carried out and sgRNAs were purified using EZNA microelute RNA Clean-UP column (OMEGA Biotek).
The Cas9 mRNA was transcribed using Pme1-digested Cas9 expression vector (http://www.addgene.org/43861/) and the mMESSAGE mMACHINE T7 ULTRA kit (Life Technologies). Following transcription, the poly(A) tailing reaction and DNase I treatment were performed according to the manufacturer’s instructions. The Cas9- encoding mRNA was then purified using Megaclear kit (Life Technologies).
Rosa26 DNA in vitro cleavage assay using TALEN and Cas9 proteins
The rat Rosa26 locus was PCR amplified (see sequences in supplementary Tables) from rat C6 line cells using specific primers. Amplified DNA was digested in the presence of TALEN ROSA-L and ROSA-R proteins or of Cas9 protein sgRNA complexes at various concentrations. Samples were analyzed on an agarose gel (2.5% TBE 0.5X) with SYBR green I post-straining.
TALEN or Cas9 mRNA activities in rat cells
The TALEN or sgRNA and Cas9 mRNAs were transfected in rat C6 cells fibroblasts using RNA MessengerMax reagent (Life Technologies). Cells were grown in 24-well dishes and collected 48 h after treatment. Cells were lysed and the genomic region encompassing the nuclease target sites was directly amplified with specific primers and fusion polymerase (New England Biolabs). T7 Endonuclease I assays were performed and estimated NHEJ frequencies were calculated as previously described34.
The rat Rosa26 DNA donor (4.7 kb) contained two 800 bp homologous arms sequences of the rat Rosa26 locus, immediately contiguous to the site of DNA cleavage (intron 1) by both TALENs and Cas9, flanking an expression cassette with the CAG promoter-eGFP cDNA-BGHpA (Fig. 2A) as has already been described in detail1. The mouse Rosa26 DNA donor (12.5 kb) contained two homologous arm sequences of the mouse Rosa26 locus of 1034 bp and 4288 bp (5 and 3′, respectively), immediately contiguous to the site of DNA cleavage (intron 1) by Cas9, flanking an expression cassette with the CAG promoter-podocan gene (Fig. 3A). The rat Foxp3 DNA donor (2.8 kb) contained two 1 kb homologous arm sequences of the rat Foxp3 locus, immediately contiguous to the site of DNA cleavage by Cas9 (exon 11, upstream of the stop codon), flanking a cassette containing a T2A self-splicing sequence and eGFP (Fig. 4A). The rat Anks3 DNA donor (100 bp) contained two homology arms of 51 and 46 bp and in between a sequence introducing a ATC to GAA mutation in position I35 and T to C mutation to obtain a conserved mutation that generates an EcoRI restriction site for the ease of analysis of HDR (Fig. 5A).
Microinjection of rat and mouse one-cell embryos
Cas9 protein and sgRNA at different concentrations indicated in the results section for each locus (for example, 3 μM of Cas9 protein corresponded to 475 ng/μl and 3 μM of sgRNA to 100 ng/μl) were incubated at room temperature for 10 min to allow formation of ribonucleoprotein complexes and then were kept at 4 °C until microinjection.
For all rat loci Cas9 mRNA was microinjected at 50 ng/μl and sgRNA at 10 ng/μl and for mouse Cas9 mRNA at 20 ng/μl and sgRNA at 10 ng/μl, concentrations close to those previously described as successfully generating mutated rats10,11,12 and mice13,14. TALEN monomer proteins were also microinjected at different concentrations (4 or 10 μM final concentration for both monomers). Mixtures of linear donor DNA (2 ng/μl) or ssODNS (7.5 ng/μl) were mixed with Cas9 (and sgRNA) or TALENs either as protein or mRNA immediately before use, briefly centrifuged at 17,950 g (10 min at 4 °C) and kept on ice during microinjection. Fertilized 1-cell stage embryo collection and sequential microinjection into the male pronucleus and into the cytoplasm have previously been described in detail1. Microinjected embryos were maintained under 5% CO2 at 37 °C 3 h. Surviving embryos were then implanted immediately in the oviduct of pseudo-pregnant females (0.5 days post coitum) and allowed to develop until embryonic day 15 for rats and day 13 for mice, or until normal delivery.
Analysis of GFP expression
GFP expression was monitored in E15 fetuses and pups using a Dark Reader® Spot Lamp (Clare Chemical Research, Inc.).
Analysis of NHEJ events
Briefly, DNA targeted regions were PCR amplified with a high-fidelity polymerase (Herculase II fusion polymerase) using specific primers (supplementary Table 1). Mutations were analyzed using the T7 endonuclease I assay and direct sequencing of PCR products as previously described in detail1,6.
Analysis of targeted HDR and random integration of DNA donor sequences
DNA from embryos or neonates was extracted from tail biopsy following treatment with Proteinase K as previously described1,6. We first identified animals carrying a transgene (GFP or others) by PCR amplification using a primer for the transgene (supplementary Table 1). Each transgene positive rat was then analyzed with PCRs flanking the donor DNA sequence using primers in the genomic sequences situated outside the homology arms and others in the inserted sequence (supplementary Table 1). These PCR fragments were then sequenced to detect genomic sequences flanking the donor DNA homology arms. For rat Rosa26, Southern-blots were also carried out on genomic DNA digested by EcoRI. After digestion, the DNA was separated by agarose gel electrophoresis, transferred to a nylon membrane (Hybond XL, GE Healthcare), hybridized with a DNA probe labeled with α32P-dCTP, washed and subjected to autoradiography.
How to cite this article: Ménoret, S. et al. Homology-directed repair in rat zygotes using Cas9 and TALEN engineered proteins. Sci. Rep. 5, 14410; doi: 10.1038/srep14410 (2015).
We thank Emmanuelle Delagoutte (Museum lab) for helpful discussion and help on recombinant protein production and purification. Funding was provided by Région Pays de la Loire through Biogenouest, the IBiSA program and Fondation Progreffe. This work received support from the French goverment, managed by the National Research Agency under the program “Investissement d’avenir” “TEFOR” (ANR-II-INSB-0014) and from the program Institut Hospitalo Universitaire-Cesti (ANR-10-IBHU-005). IHU-CESTI is also supported by Nantes Metropole and the Pays de la Loire Region. Sequencing experiments were performed by the integrative genomic facility of Nantes.
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Optimization of the production of knock-in alleles by CRISPR/Cas9 microinjection into the mouse zygote
Scientific Reports (2017)