Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Endurance training facilitates myoglobin desaturation during muscle contraction in rat skeletal muscle

Abstract

At onset of muscle contraction, myoglobin (Mb) immediately releases its bound O2 to the mitochondria. Accordingly, intracellular O2 tension (PmbO2) markedly declines in order to increase muscle O2 uptake (mO2). However, whether the change in PmbO2 during muscle contraction modulates mO2 and whether the O2 release rate from Mb increases in endurance-trained muscles remain unclear. The purpose of this study was, therefore, to determine the effect of endurance training on O2 saturation of Mb (SmbO2) and PmbO2 kinetics during muscle contraction. Male Wistar rats were subjected to a 4-week swimming training (Tr group; 6 days per week, 30 min × 4 sets per day) with a weight load of 2% body mass. After the training period, deoxygenated Mb kinetics during muscle contraction were measured using near-infrared spectroscopy under hemoglobin-free medium perfusion. In the Tr group, the mO2peak significantly increased by 32%. Although the PmbO2 during muscle contraction did not affect the increased mO2 in endurance-trained muscle, the O2 release rate from Mb increased because of the increased Mb concentration and faster decremental rate in SmbO2 at the maximal twitch tension. These results suggest that the Mb dynamics during muscle contraction are contributing factors to faster O2 kinetics in endurance-trained muscle.

Introduction

Relative to control muscle, endurance-trained muscle increases O2 consumption at the same level of maximal voluntary contraction (MVC) and increases maximal O2 consumption, which is considered an indicator of improved aerobic exercise capacity. The increased O2 consumption in the trained skeletal muscle depends on both O2 utilization and vascular O2 supply. Muscle O2 utilization capacity is mainly determined by mitochondrial function and the quantity of mitochondria, whereas O2 supply capacity to the mitochondria is determined by capillarization. Many studies have reported that endurance training upregulates mitochondrial function and mitochondria number and volume1,2,3. It also increases capillarization1,2,3. However, the contribution of O2 diffusion from the capillary to the mitochondria is still unknown, especially with respect to the intracellular factors involved in O2 transport from the sarcolemma to the mitochondria.

Recent studies have shown that the O2 gradient can contribute to the enhanced O2 flux to meet the increased muscle O2 demand during contraction4. The O2 saturation of Mb (SmbO2), which reflects the intracellular O2 tension (PmbO2), decreases as work intensity and muscle oxygen consumption (mO2) increase. The decreasing PmbO2 expands the O2 gradient from the capillary to the muscle cell to increase the O2 flux from the vasculature to the mitochondria. Whether the O2 gradient contributes to the increased O2 uptake in endurance-trained muscle remains uncertain. With the experimental model to investigate the intracellular O2 dynamics4, we have hypothesized that the increase in O2 consumption in endurance-trained skeletal muscle is accompanied with increased expansion of the O2 gradient across the plasma membrane4, because studies have already shown that the change in PmbO2 during exercise can play a key role in O2 regulation5,6.

Because training also induces an acceleration of O2 kinetics at the onset of muscle contraction, which pulmonary O2 measurements have detected and have attributed to adjustments of oxidative metabolism at the skeletal muscle level7,8,9, a faster O2 on-kinetics could be an important adaptation, as it would potentially incur a smaller O2 deficit. Previous studies have already shown Mb contribution to the intracellular O2 dynamics, which affects the mO2 response at the onset of muscle contraction4,5,10,11. Nuclear magnetic resonance and near-infrared spectroscopic (NIRS) experiments clearly show that Mb immediately releases O2 at the onset of muscle contraction and provides the initial O2 supply to support the rapid increase in mO24,5,10,11. The sudden increase in mO2 does not appear to depend on muscle adenosine diphosphate concentration and thereby implicates a direct and immediate role for Mb-mediated O2 delivery5.

By using a hemoglobin (Hb)-free rat hind limb perfusion model, the present study shows that at all relative levels of MVC, the mO2 of endurance-trained muscle exceeds that of the control muscle. Trained muscle also reaches a higher peak mO2. Even though the PmbO2 of both the control and endurance-trained muscle decreases with increasing exercise intensity, the O2 gradient from capillary to the mitochondria does not change significantly to accommodate the differences in the observed mO2. At any given MVC, the endurance-trained muscle exhibits a smaller O2 gradient. However, the endurance-trained muscles exhibit a faster release of O2 from Mb at the initiation of contraction, consistent with the enhanced mO2 and with the Mb-mediated O2 supply. Indeed the kinetics of O2 release from Mb can serve as an index of the change in intracellular mO2 as muscle undergoes training5.

Results

Descriptive data for muscle weight are presented in Table 1. Although endurance training caused a significant reduction in body and muscle mass, the ratio of muscle mass to body mass differed slightly between the groups (with a difference in mean value of 0.1%).

Table 1 Descriptive data for the muscle weight

Table 2 shows the contractile and metabolic properties of the control and trained hind limb muscles. Although both groups showed no significant difference in mO2 at rest, at maximal tension, the values of the peak mO2 per gram per minute in the swimming training (Tr) group were significantly higher than those in the control (Con) group. [Mb] and citrate synthase (CS) activities in the deep portion of gastrocnemius muscle significantly increased after endurance training, whereas the lactate-to-pyruvate ratio (L/P) decreased at peak maximal twitch tension. Table 3 summarizes muscle tension, the net increase in mO2 due to muscle contraction (ΔmO2), O2 cost and kinetics parameters for SmbO2 and PmbO2 at each tension level for both groups. Muscle tension, ΔmO2 and O2 cost increased as work intensity increased.

Table 2 Contractile and metabolic properties of hindlimb muscles
Table 3 Muscle tension, muscle oxygen consumption, SmbO2 and PmbO2 kinetics parameters during muscle contraction at each tension level

Figure 1 shows the representative kinetics of SmbO2 in each group. As for the SmbO2 kinetic parameters, the steady-state value, amplitude (AP) and mean rate of change to 63% of the AP value (0.63AP/mean response time [MRT]) increased as work intensity increased in both groups, whereas MRT tended to accelerate in both groups. At maximal tension, the steady-state value and AP of SmbO2 kinetic parameters in the Tr group were unchanged, but 0.63AP/MRT of the kinetic parameters for SmbO2 increased. The MRT also tended to be faster in the Tr group. At submaximal tension levels, the steady-state value, AP and 0.63AP/MRT in the Tr group were also unchanged, but the MRT of the kinetic parameters for SmbO2 tended to be faster. The kinetic parameters for PmbO2 showed that the steady-state value, AP and 0.63AP/MRT increased and the MRT became faster in both groups as work intensity increased. When the kinetic parameters for PmbO2 were compared at the same relative tension level between both groups, the relative temporal parameters for PmbO2 kinetics in the trained muscle showed a tendency to accelerate to a higher level. In the present study, while the MRT was used to describe the overall dynamics of SmbO2 and PmbO2 fall following the onset of muscle contraction, 0.63AP/MRT is the effective temporal parameter to show deoxygenation rate of Mb-O2 per unit time in the initial phase of muscle contraction. The 0.63AP/MRT would reflect the steep change in mitochondrial oxygen demand, because we previously reported that 0.63AP/MRT increased in response to change in mitochondrial oxygen demand due to muscle contraction. Kindig et al.12 also used AP/time constant in intracellular PO2 kinetics during muscle contraction as an index of initial metabolic response. As for 0.63AP/MRT parameter in the present study, while its value showed significant difference at 100% of the maximal twitch tension by endurance training, it did not differ at 50% and 75% of the maximal twitch tension between Con and Tr group. This result at submaximal tension level might be caused by non-significant difference in O2 demand level during muscle contraction at the relative same intensity between groups. ΔmO2 did not actually show the significant difference at the 50% and 75% of the maximal twitch tension between groups. On the other hand, at the maximal twitch tension level, 0.63AP/MRT parameter and ΔmO2 in the trained muscle showed significant difference compared with that in the control muscle.

Figure 1
figure 1

Representative kinetics of myoglobin (Mb) saturation (SmbO2) during maximal twitch contraction (1 Hz) in the training (Tr) and control (Con) groups.

The plots of SmbO2 show representative data at the maximal twitch tension from the single experiment in each group. While the SmbO2 kinetics (dotted line) in the representative control rat declined with a mean response time (MRT) of 39.5 sec (upper panel), the SmbO2 kinetics (solid line) in the representative trained rat declined with an MRT of 33.0 sec (lower panel). The MRT in the SmbO2 kinetics was shortened by 5 sec on average due to endurance training. By contrast, the SmbO2 value at steady state did not show any significant difference between the two groups.

Figure 2 shows the relationship between muscle tension and the net increase in mO2 during muscle contraction for both groups. While muscle tension and ΔmO2 were significantly correlated in both groups, the mean individual slope in the Tr group (0.36 ± 0.11 × 10−2 μmol/[g2·min]) tended to be higher than that in the Con group (0.27 ± 0.06 × 10−2 μmol/[g2·min]; p = 0.058).

Figure 2
figure 2

Relationship between muscle tension and ΔmO2 during twitch contraction in the training (Tr) and control (Con) groups.

Changes in muscle O2 uptake (ΔmO2) due to muscle contraction increased linearly as a function of muscle tension in both groups. Regression lines are based on mean values (n = 9 in each group; Con: ΔmO2 = 0.003 × Tension, R2 = 0.98, p < 0.05; Tr: ΔmO2 = 0.004 × Tension, R2 = 0.99, p < 0.05). The data represent the mean ± standard deviation values. The superscript indicates a significant difference (a: vs. Tr × 100%, p < 0.05; b: vs. Tr × 75%, p < 0.05; c: vs. Con × 100%, p < 0.05).

Figure 3 shows the relationship between intracellular [O2] and ΔmO2 during muscle contraction. Intracellular [O2] was based on the SmbO2–PmbO2 equilibrium. In the present study, the SmbO2 at rest was assumed to be 90%. Intracellular [O2] decreased markedly from 29.2 μM at rest to 9.2 ± 3.0, 5.1 ± 2.1 and 3.3 ± 1.0 μM at 50%, 75% and 100% of maximal contraction in the Con group, respectively; and from 29.2 μM at rest to 12.1 ± 4.7, 5.8 ± 1.9 and 3.2 ± 0.7 μM at 50%, 75% and 100% of maximal contraction in the Tr group, respectively. Although intracellular [O2] decreased markedly with the ΔmO2 in both groups, the Tr group curve showed a smaller [O2] decline. At the same level of intracellular [O2] in the muscle cell, ΔmO2 in the trained muscle was higher than in the control muscle, suggesting that the trained muscle had more oxidative potential capacity compared with the control muscle.

Figure 3
figure 3

Relationship between intracellular [O2] and ΔmO2 during twitch contraction in the training (Tr) and control (Con) groups.

Intracellular [O2] (in μM) decreased gradually with the increase in changes in muscle O2 uptake (ΔmO2) in both groups. The relationship between intracellular [O2] and ΔmO2 was shown as a line graph. Each data point represents the mean ± standard deviation. The superscript letters indicate significant differences (a: vs. Tr × 100%, p < 0.05; b: vs. Tr × 75%, p < 0.05; c: vs. Con × 100%, p < 0.05; and d: vs. Con × 75%).

Figure 4 shows the O2 release rate from Mb at same percent of MVC in the Tr and Con groups. The O2 release rate from Mb increased progressively with the twitch tension level as follows: 1.1 ± 0.3, 2.3 ± 0.4 and 3.7 ± 0.8 × 10−2 μmol/(g·min) at 50%, 75% and 100% of maximal contraction in the Con group, respectively; and 1.1 ± 0.5, 2.6 ± 0.7 and 4.6 ± 0.5 × 10−2 μmol/(g·min) at 50%, 75% and 100% of maximal contraction in the Tr group, respectively. At maximal tension, the O2 release rate from Mb showed a significant increase in the Tr group, suggesting more O2 supply from Mb to the mitochondria at the onset of muscle contraction.

Figure 4
figure 4

The O2 release rate from myoglobin (Mb) at the each tension level in the training (Tr) and control (Con) groups.

At the onset of muscle contraction, Mb released its bound O2 to the mitochondria with the twitch tension level. The data show the mean ± standard deviation values. The superscript letters indicate significant differences (a: vs. Tr × 100%, p < 0.05; b: vs. Tr × 75%, p < 0.05; c: vs. Con × 100%, p < 0.05; and d: vs. Tr × 50%, p < 0.05).

Discussion

Effect of endurance training on muscle oxidative capacity

In the present study, 4 weeks of swimming endurance training resulted in an increase in mO2 peak, even when O2 delivery to the endurance-trained hind limb was not greater than that supplied to sedentary muscles. This increase in mO2 peak value at constant flow was consistent with previous studies2,15. This increase in mO2 peak value without increase in O2 delivery to the hind limb muscle would be caused by increased of both O2 supply capacity to the mitochondria and O2 utilization capacity such as capillary density, mitochondrial respiration capacity and Mb function in the active muscle. At the equivalent muscle tension, the Tr group showed a slightly higher ΔmO2 than the Con group (Fig. 2). As reflected by a higher CS activity, the endurance-trained muscle had higher muscle oxidative potential. The increase in O2 consumption and O2 cost at a given work rate would imply a shift to more aerobic metabolism during muscle contraction.

The decrease in L/P at the maximal twitch tension also suggested a greater capacity to oxidize carbohydrate and a tightening in the coupling between ATP supply and demand13,14. This tight integration of ATP supply and demand is associated with less stimulation of glycolysis, resulting in a decrease in lactate production and a lower cytosolic redox state and thus an improved coupling between pyruvate oxidation and glycolytic flux13,15. Collectively, the swimming endurance training in the present study enhanced muscle oxidative capacity, in agreement with evidence previous studies1,16,17.

Relationship between PmbO2 kinetics and muscle oxygen consumption

Endurance training increases both mO2 peak and ΔmO2 at the same percentage of MVC. However, both control and trained muscle show a steady decline in PmbO2 with increasing MVC. The declining PmbO2 and the increasing O2 consumption indicates an expansion of the O2 gradient across the plasma membrane. Both the O2 diffusion conductance (DO2) and O2 gradient between PcapO2 and PmbO2 can influence the O2 flux into the cell, which then supports the mO2. In our perfusion model, the O2 diffusion conductance would show little change even at the onset of contraction. Fick's first law of diffusion relates explicitly the change in substance concentration over time depends upon the gradient of concentration over space. In a one-dimension case for O2 diffusion in the x-direction, the equation clearly states that:

J = diffusion flux (amount of O2 crossing a unit area per unit time), D = diffusion coefficient (length of unit area squared x time−1), = the change in O2 concentration along dimension x or the O2 gradient along the x-direction. 1H-NMR experiments show Mb desaturating and the cellular PO2 decreasing rapidly upon the initiation of muscle contraction5,6. The debate remains whether with increasing exercise intensity and associated increasing respiration, does the gradient expand or does it reach a plateau. Our experiment data show the gradient expanding. Conductance may still contribute to O2 transport into the cell. However, our experiments provide no supporting or contradictory data. They only show that blood flow has not changed. Indeed, we used the constant flow mode and an Hb-free Krebs-Henseleit buffer as perfusate in this perfusion experiment. By using the constant flow mode, the perfusion pressure remained constant throughout the perfusion period, which suggested the convective O2 delivery did not change both at rest and during muscle contraction. Additionally, based on the fact that a 30-min equilibrium period elicited a flow-induced vasodilatation corresponding to the given flow rate18, we assumed that convective O2 delivery was maintained during the perfusion period. Also, as the perfusate did not contain an Hb in this study, muscle contraction would have little affect to skeletal muscle capillary hemodynamic in contrast to the previous study of Kindig et al.19 that muscle contraction induced rapid increase of RBC flux and velocity and capillary hematocrit at the microcirculatory level. Thus, the supposition of a requisite and predominant conductance role in diffusion cannot undermine the observation of an expanding O2 gradient that coincides with an increased mO2.

Because the steady-state PcapO2 level doesn't change20 and the PmbO2 decreases with increasing exercise intensity, the increase in ΔmO2 and mO2 peak in the Tr group must also have a contribution from an increased capillarization and/or a greater DO21. In the extracellular segment, the angiogenesis of the muscle capillary in the hind limb muscles during training would enhance the supply of O2. An increase in capillarity would improve blood flow-to-O2 relationships within the muscle, allowing for a greater O2 extraction1. The change in capillarity can also affect DO2. A greater DO2 after exercise training implies a decreased mean O2 diffusion distance from the tissue capillaries to the muscle mitochondria21,22. In the intracellular segment, O2 transport to the mitochondria depends upon Mb-mediated O2 flux and free O2. The Mb-mediated O2 flux in the trained muscle would show a greater value at a relatively higher tension level where intracellular O2 tension (equivalent with PmbO2) became lower, because an adaptive increase in Mb concentration led to the increase in Mb-O2 flux, as also shown by a previous study23. Therefore, increased augmentation of capillarity and Mb-mediated O2 flux during muscle contraction can enhance the capacity supplying O2 to the mitochondria to support the increased ΔmO2 and mO2 peak after endurance training. The experiment results show that the hypothesized expansion of O2 gradient due to further decrease in PmbO2 does not explain the overall increase in ΔmO2 and mO2 peak in endurance-trained muscle.

O2 release rate from Mb at the onset of muscle contraction

Endurance training usually results in faster O2 kinetics24, which will presumably experience result in a smaller decrease in muscle phosphocreatine concentration, a smaller increase in lactate and proton (H+) production and a reduced degradation of muscle glycogen, compared with an individual with slow O2 on-kinetics25,26,27. Improvement in mitochondrial respiration capacity itself would largely contribute to this adaptation as a result of endurance training. However, no study has investigated the O2 supply to the mitochondria at the intracellular level.

We previously found that Mb supplied O2 immediately at the onset of muscle contraction and that the O2 release rate from Mb increased linearly as the O2 demand increased4,28. These facts suggest that Mb provides an immediate O2 source for the sudden increase in mO2 at the onset of muscle contraction. The present study reveals an increase in the O2 release rate from Mb at the onset of muscle contraction at the maximal twitch tension after endurance training. Myocyte experiments have also suggested that a direct Mb-mediated oxygen delivery might contribute to mitochondrial respiration29. The blockade of Mb oxygen-binding capacity suppressed approximately 70% of mitochondrial respiration, even under the condition of sufficiently available O229. Indeed, the fact that the O2 release rate from Mb at the onset of muscle contraction increases progressively as O2 demand increases might indicate that Mb-supplied O2 may directly influence mO2 kinetics4. Taking these findings together, both mitochondrial respiration capacity and O2 release rate from Mb might be important factors that regulate mO2 kinetics at the onset of muscle contraction.

The binding of O2 to Mb and Hb certainly proceeds much faster than transport30,31, even though the rate-determining step depends on a much slower off rate. But, dismissing any contribution of Mb in regulating respiration in the cell (an inhomogeneous and compartmentalized system) based on just the steady-state rate determining step argument seems tenuous. If the blood delivers a sufficient O2 supply, the cell would not need to withdraw O2 from its Mb reservoir at the start of muscle contraction. But the cell does withdraw O2 from Mb, as our data and all 1H-NMR data show and takes a finite amount of time to reach a new steady state5,6. Thermodynamics requires a demand to elicit the loss of O2 from Mb and both ATP utilization and respiration surge once contraction starts. The kinetics coincides with O2 release from Mb. Thus, the postulated regulatory relationship between the O2 release rate from Mb and mO2 seems quite reasonable and consistent with the postulated role of Mb. Once Mb desaturation has reached a new steady state, vascular O2 supply must begin to contribute significantly to sustain the rising mO2. To avoid the missteps in the rate limiting step approach, metabolic control theory vantage advocates examining the relative contribution of MbO2 and O2 to the regulation of mO2. Note that Mb never resaturates to its control level as long as the muscle contraction is sustained. The cellular PO2 drops during contraction, consistent with an enhanced O2 gradient.

The O2 release rate from Mb reflects the intracellular mO25,6. Consequently, the enhanced intracellular mO2 observed after endurance training could be induced by a 30% increase in [Mb] concentration and a 12% acceleration in the Mb deoxygenation rate at the maximal twitch tension. Tables 2 and 3 show that an increase in [Mb] predominantly contributes to an increase in O2 release rate from Mb in the trained skeletal muscles. Although several studies have reported that endurance training produces an increased [Mb] in rat limb muscle16,17,32,33, the physiological significance of increased [Mb] has not been demonstrated in vivo. The present study also demonstrated that the deoxygenation rate of Mb became faster at the onset of muscle contraction after endurance training, suggesting a more efficient O2 transport from Mb to the mitochondria at the transient phase.

In addition, the PmbO2 response at the onset of muscle contraction showed the tendency to be faster after endurance training in the present study. Hirai et al.20 reported that endurance training led to slower PcapO2 kinetics during 1-Hz twitch contraction, indicating a relatively greater increase in muscle blood flow at the microvascular level than in O2 diffusivity. This adaptation would be mainly caused by an increase in capillary density. Meanwhile, the intracellular O2 environment was reported to adjust more effectively to the abrupt increase in oxygen demand at the onset of muscle contraction, before the microcirculatory O2 environment has adapted4. In fact, the MRT of PmbO2 kinetics became approximately 5 seconds faster on average by endurance training. The acceleration of PmbO2 kinetics with slower PcapO2 kinetics would imply a sharper expansion of O2 gradient at the onset of muscle contraction, resulting in a more efficient O2 transport from Mb to the mitochondria at the transient phase.

In the present study, we have performed additional statistical analyses on kinetics parameters to check the existence of type II error. As for MRT in PmbO2 kinetics, the effect size was 0.052 and the statistical power was 0.109. This level of statistical power implies the existence of a type II error. However, based on our experimental content, increasing the sample size would not necessarily improve the accuracy or precision of our results. This might be one of limitations in this type of experiment. Actually, although significant difference was not recognized for kinetics parameters such as MRT between groups at the relative same tension level, the shorting of MRT by 5 sec on average at the maximal tension level in both of SmbO2 and PmbO2 kinetics suggests the possibility that swimming endurance training accelerates both kinetics during muscle contraction.

In summary, the results presented herein suggest that Mb plays an important role in the faster mO2 response and increased mO2 in trained skeletal muscles. However, how Mb-bound O2 is supplied to the mitochondria at the onset of muscle contraction remains unclear. Recently, Yamada et al.34 suggested the possibility that the presence of Mb in mitochondrial fractions indicates involvement in the immediate O2 release from Mb at the onset of muscle contraction. If so, endurance training might impact Mb localization in the muscle cell. Further research is required to elucidate the mechanism of O2 transport to the mitochondria within muscle cells and the causal relationship between cellular factors altering the O2 off rate in Mb and mitochondrial respiration activity.

Methods

Experimental Animals and Preparation of Hindlimb Perfusion

Male Wistar rats were employed as subjects. All were housed in a temperature-controlled room at 23 ± 2°C with a 12-h light–dark cycle and maintained on a commercial diet with water ad libitum. The procedures conformed to the “Fundamental Guidelines for Proper Conduct of Animal Experiment and Related Activities in Academic Research Institutions” (published by the Ministry of Education, Culture, Sports, Science and Technology, Japan) and was approved by the Ethics Committee for Animal Experimentation of Kanazawa University (Protocol AP-101636).

Five-week-old Wistar rats were randomly divided into the Con and 4 weeks Tr groups (n = 9 in each group). The training protocol for the swimming group was as follows: On the first and second days, the rats swam for 1 h in two 30-min bouts separated by 5 min of rest. On the third and fourth days, the rats swam for 1.5 hours in three 30-min bouts separated by 5 min of rest. On and after the fifth day, the rats swam for 2 hours in four 30-min bouts separated by 5 min of rest. Except for the first bout of swimming training until the sixth day, a weight equal to 2% of the rats' body weight was tied to the bodies of the rats. The rats performed the above swimming protocol six days per week. During swimming exercise, the water temperature was kept at around 35°C. The tank's shape was square and its characteristics were 48 cm depth, 80 cm longitudinal and 60 cm width. All rats swam in that tank and an average surface area of at least 600 cm2/rat. Also, we kept monitoring to prevent the climbing, diving and bobbing of rat during swimming training. In cases where these behaviors were observed, they were dealt with immediately.

After 4 weeks (at 9-week of age), hindlimb perfusion was performed in each group (Con group: initial body weight (BW) at 5 weeks old; 143–168 g, final BW at 9 weeks old; 257–295 g, Tr group: initial BW; 140–176 g, final BW; 226–250 g). Preparation of isolated rat hindlimb and the perfusion apparatus are described in previous reports4,28. All surgical procedures were performed under pentobarbital sodium anesthesia (45 mg kg−1 intraperitoneal). The rats were killed by injecting 1 M KCl solution directly into the heart, followed by a surgical procedure and an Hb-free Krebs-Henseleit buffer (NaCl, 118 mM; KCl, 5.9 mM; KH2PO4, 1.2 mM; MgSO4, 1.2 mM; CaCl2, 1.8 mM; NaHCO3, 20 mM; Glucose, 15 mM) equilibrated with 95%O2 + 5%CO2 at 37°C was perfused into the abdominal aorta in flow through mode, at a constant flow rate. In order to adjust the perfusion pressure to approximately 80.0 mmHg, the flow rate was set to 22.0 ± 0.0 ml min−1 in the Con group and 22.1 ± 0.9 ml min−1 in the Tr group. In this condition, the average perfusion pressures were 78.6 ± 7.7 mmHg in the Con group and 74.2 ± 5.2 mmHg in the Tr group. Therefore, the perfusion resistance was unchanged throughout the perfusion period. In addition, no sign of oedema in the hindlimb was seen at the given flow rate. The effluent was collected from the inferior vena cava in order to measure mO2 and the lactate and pyruvate concentrations.

Measurement Parameters

The twitch contraction protocol and measurement of Mb oxygenation and mO2 followed the previous methods4,28. The sciatic nerve of the left hindlimb was then exposed and connected to two parallel stainless steel wire electrodes (Unique Medical, Tokyo, Japan) and the Achilles' tendon was connected to a sensitive strain gauge with a string (MLT500/D, AD Instrument, Castle Hill, NSW, Australia). The stimulation pulse via the sciatic nerve derived by an electrostimulator system (Model RU-72, Nihon Koden, Tokyo, Japan) was 1 Hz in frequency (delay, 10 μsec; duration, 1 msec) for 120 sec (120 twitch contractions). Target tension was controlled by changing the voltage of stimuli to obtain 50%, 75% and 100% of peak tension under buffer-perfused conditions (3–8 volts). Twitch tension was calculated as the average of a series of contractions. The muscle also showed no sign of fatigue, even at the highest stimulation intensity.

An NIRS instrument (NIRO-300 + Detection Fibre Adapter Kit, Hamamatsu Photonics, Shizuoka, Japan) was employed to measure oxygenation of Mb at rest and during muscle contraction. The distance between the photodiode and the LED was fixed at 10 mm. The toe of the foot was secured by a clamp with the rat laid on its back. After that, the NIRS probes were firmly attached to the skin of the gastrocnemius muscle and were fixed by clamps on both sides of the muscle. During the initial period, for at least 30 sec before the start of contraction, the average fluctuation in the NIRS signals was adjusted to a reference value of zero. After the exercise protocol, the anoxic buffer (equilibrated with 95% N2 + 5% CO2 gas) was perfused for 30 min to obtain maximal Mb desaturation. The muscle then received electrical stimulation to contract for 2 min. No further increase in change in NIRS signal associated with concentration of deoxygenated Mb (Δ[deoxy-Mb]) signal was evident. The final Δ[deoxy-Mb] signal intensity served as the normalization constant for 100% Mb deoxygenation.

mO2 (μmol g−1 min−1) was calculated from the arteriovenous O2 content differential multiplied by flow rate, using two O2 electrodes (5300A, YSI, Yellow Springs, Ohio, USA). Two O2 electrodes were adjusted for the vapour pressure of water before hindlimb perfusion experiment. The oxygen cost was calculated by dividing change in mO2 due to muscle contraction by muscle tension. The L/P remains constant and shows no significant increase from the resting muscle value. Lactate levels can increase for a number of reasons, including lack of adequate O2 delivery. Therefore, limited oxygen availability should lead to an increase in lactate level as anaerobic glycolysis starts. Indeed, the L/P increases during perfusion with anoxia buffer (95%N2) increases. O2 availability and delivery can sustain maximal contraction with no sign of fatigue.

The sampling rate for the NIRS data was 1 Hz. The other parameters (tension, perfusion pressure, O2 content at the inflow and outflow) were collected using a data acquisition system (PowerLab 8SP, AD Instruments, Australia) at a sampling rate of 1 kHz. All the data were transferred to a personal computer with acquisition software (Chart ver. 5.5.6. AD Instruments).

Data Analysis

The data analysis followed our previous methods4. A simple moving average smoothed the Δ[deoxy-Mb] and Δ[oxy-Mb] NIRS signals using a rolling average of 5 points, which corresponds to a 5-sec timeframe35. The Δ[deoxy-Mb] signals were calibrated against two different NIRS signal values: one at rest as 10% Mb deoxygenation and the other during steady state with anoxic buffer perfusion as 100% Mb deoxygenation. While the SmbO2 at rest could not be determined by NIRS, the value was assumed to be 90% based on previous studies reporting that the SmbO2 at rest was greater than 90%5,36.

The %Δ[deoxy-Mb] plots were converted to SmbO2 (%) plots using the following equation:

SmbO2 plots were fitted by the following single-exponential equation to calculate kinetics parameters using an iterative least-squares technique by means of a commercial graphing/analysis package (KaleidaGraph 3.6.1, Synergy Software, Reading, PA, USA):

where BL is the baseline value, AP the amplitude between BL and the steady-state value during the exponential component, TD the time delay between onset of contraction and appearance of SmbO2 signals and τ the time constant of SmbO2 signal kinetics. MRT calculated by TD + τ was used as an effective parameter of the response time for Mb deoxygenation at onset of muscle contraction. Dividing 63% of AP by MRT yields a value for the time-dependent change in Mb deoxygenation. These parameter, 0.63AP/MRT, for SmbO2 shows the O2 release rate from Mb, which indicates the amount of O2 released from Mb per unit time at onset of exercise. The O2 release rate from Mb was calculated using the following equation:

where 0.63AP/MRT for SmbO2 was the Mb deoxygenation rate in %/sec. Inserting this value for Mb into the equation led to determination of the O2 release rate from Mb in micromoles per gram per minute.

We reconstructed PmbO2 kinetics based on the resulting SmbO2 kinetics parameters. The model SmbO2 kinetics was converted to PmbO2 (mmHg) using the following equation:

where P50 is the partial oxygen pressure required to half-saturate Mb. A P50 of 2.4 mmHg was used for this equation, assuming a muscle temperature of 37°C37. The calculated PmbO2 plots were evaluated to obtain an MRT of its kinetics using the same single exponential equation as for PmbO2. The 0.63AP/MRT for PmbO2 indicates a rate of decrease in PmbO2 at muscle contraction onset. PmbO2 at steady state was calculated by using the SmbO2 value at steady state. Since O2 partial pressure corresponds to a specific amount of dissolved O2, intracellular [O2] (μM) was calculated from the PmbO2 value at rest and at each exercise intensity using the following equation:

with PmbO2 is in mmHg and O2 solubility in buffer is 0.00135 μmol ml−1 mmHg−1 at 37°C38.

Mb Concentration and CS Activity in Buffer-Perfused Muscle Tissue

After buffer perfusion experiment, Mb concentration in muscle tissue was measured by a modified Reynafarje method39. CS activity, a mitochondrial enzyme and marker of muscle oxidative potential, was measured in whole muscle homogenates by using the spectrophotometric method of Srere40.

Statistical Analyses

All data are expressed as mean ± SD. Statistical differences were examined using two-way unpaired measures analysis of variance (ANOVA) (tension level × training). A Turkey-Kramer post-hoc test was applied if the ANOVA indicated a significant difference. An unpaired t-test was used in comparing biochemical and physiological parameters between groups. Pearson's correlation coefficient was calculated when the relationship between two variables was evaluated. The level of significance was set at p < 0.05.

References

  • Bebout, D. E., Hogan, M. C., Hempleman, S. C. & Wagner, P. D. Effects of training and immobilization on O2 and DO2 in dog gastrocnemius muscle in situ. J Appl Physiol 74, 1697–1703 (1993).

    CAS  Google Scholar 

  • Burelle, Y. & Hochachka, P. W. Endurance training induces muscle-specific changes in mitochondrial function in skinned muscle fibers. J Appl Physiol 92, 2429–2438 (2002).

    Article  Google Scholar 

  • Charifi, N. et al. Enhancement of microvessel tortuosity in the vastus lateralis muscle of old men in response to endurance training. J Physiol 554, 559–569 (2004).

    CAS  Article  Google Scholar 

  • Takakura, H., Masuda, K., Hashimoto, T., Iwase, S. & Jue, T. Quantification of myoglobin deoxygenation and intracellular partial pressure of O2 during muscle contraction during haemoglobin-free medium perfusion. Exp Physiol 95, 630–640 (2010).

    CAS  Article  Google Scholar 

  • Chung, Y. et al. Control of respiration and bioenergetics during muscle contraction. Am J Physiol Cell Physiol 288, C730–C738 (2005).

    CAS  ADS  Article  Google Scholar 

  • Molé, P. A. et al. Myoglobin desaturation with exercise intensity in human gastrocnemius muscle. Am J Physiol 277, R173–R180 (1999).

    PubMed  Google Scholar 

  • Rossiter, H. B. et al. Inferences from pulmonary O2 uptake with respect to intramuscular [phosphocreatine] kinetics during moderate exercise in humans. J Physiol 518, 921–932 (1999).

    CAS  Article  Google Scholar 

  • Grassi, B. et al. Muscle O2 uptake kinetics in humans: implications for metabolic control. J Appl Physiol 80, 988–998 (1996).

    CAS  Article  Google Scholar 

  • McCreary, C. R. et al. Kinetics of pulmonary oxygen uptake and muscle phosphates during moderate-intensity calf exercise. J Appl Physiol 81, 1331–1338 (1996).

    CAS  Article  Google Scholar 

  • Behnke, B. J. et al. Dynamics of oxygen uptake following exercise onset in rat skeletal muscle. Respir Physiol Neurobiol 133, 229–239 (2002).

    Article  Google Scholar 

  • Behnke, B. J., McDonough, P., Padilla, D. J., Musch, T. I. & Poole, D. C. Oxygen exchange profile in rat muscles of contrasting fibre types. J Physiol 549, 597–605 (2003).

    CAS  Article  Google Scholar 

  • Kindig, C. A., Howlett, R. A. & Hogan, M. C. Effect of extracellular PO2 on the fall in intracellular PO2 in contracting single myocytes. J Appl Physiol 94, 1964–1970 (2003).

    Article  Google Scholar 

  • Holloszy, J. O. & Coyle, E. F. Adaptations of skeletal muscle to endurance exercise and their metabolic consequences. J Appl Physiol 56, 831–838 (1984).

    CAS  Article  Google Scholar 

  • Phillips, S. M., Green, H. J., Tarnopolsky, M. A., Heigenhauser, G. J. & Grant, S. M. Progressive effect of endurance training on metabolic adaptations in working skeletal muscle. Am J Physiol 270, E265–E272 (1996).

    CAS  Article  Google Scholar 

  • Phillips, S. M. et al. Effects of training duration on substrate turnover and oxidation during exercise. J Appl Physiol 81, 2182–2191 (1996).

    CAS  Article  Google Scholar 

  • Hickson, R. C. Skeletal muscle cytochrome c and myoglobin, endurance and frequency of training. J Appl Physiol 51, 746–749 (1981).

    CAS  Article  Google Scholar 

  • Masuda, K., Kano, Y., Nakano, H., Inaki, M. & Katsuta, S. Adaptation of myoglobin in rat skeletal muscle to endurance running training -effects of intensity, duration and period of training-. The Japanese Society of Physical Fitness and Sport Medicine 47, 561–572 (1998).

    Article  Google Scholar 

  • Hepple, R. T., Krause, D. J., Hagen, J. L. & Jackson, C. C. O2max is unaffected by altering the temporal pattern of stimulation frequency in rat hindlimb in situ. J Appl Physiol 95, 705–711 (2003).

    Article  Google Scholar 

  • Kindig, C. A., Richardson, T. E. & Poole, D. C. Skeletal muscle capillary hemodynamics from rest to contractions: implications for oxygen transfer. J Appl Physiol 92, 2513–2520 (2002).

    Article  Google Scholar 

  • Hirai, D. M. et al. Exercise training and muscle microvascular oxygenation: functional role of nitric oxide. J Appl Physiol 113, 557–565 (2012).

    CAS  Article  Google Scholar 

  • Hudlicka, A. Handbook of Physiology. Section 2: The Cardiovascular System, Vol IV, Microcirculatio [Renkin E. M. & Michel C. C. (ed.)] (American Physiological Society, Bethesda, 1984).

  • Saltin, B. & Gollnick, P. D. Skeletal muscle adaptability: significance for metabolism and performance. [Peachey L. D. & Geiger C. C. (ed.)] (American Physiological Society, Bethesda, 1983).

  • Lin, P. C., Kreutzer, U. & Jue, T. Myoglobin translational diffusion in rat myocardium and its implication on intracellular oxygen transport. J Physiol 578, 595–603 (2007).

    CAS  Article  Google Scholar 

  • Berger, N. J., Tolfrey, K., Williams, A. G. & Jones, A. M. Influence of continuous and interval training on oxygen uptake on-kinetics. Med Sci Sports Exerc 38, 504–512 (2006).

    Article  Google Scholar 

  • Cerretelli, P., Pendergast, D., Paganelli, W. C. & Rennie, D. W. Effects of specific muscle training on O2 on-response and early blood lactate. J Appl Physiol 47, 761–769 (1979).

    CAS  Google Scholar 

  • Demarle, A. P. et al. Decrease of O2 deficit is a potential factor in increased time to exhaustion after specific endurance training. J Appl Physiol 90, 947–953 (2001).

    CAS  Article  Google Scholar 

  • Jones, A. M. & Koppo, K. Oxygen Kinetics Uptake in Sport, Exercise and Medcine. (Routledge, Oxon, 2005).

  • Masuda, K., Takakura, H., Furuichi, Y., Iwase, S. & Jue, T. NIRS measurement of O2 dynamics in contracting blood and buffer perfused hindlimb muscle. Adv Exp Med Biol 662, 323–328 (2010).

    CAS  Article  Google Scholar 

  • Wittenberg, B. A. & Wittenberg, J. B. Myoglobin-mediated oxygen delivery to mitochondria of isolated cardiac myocytes. Proc Natl Acad Sci USA 84, 7503–7507 (1987).

    CAS  ADS  Article  Google Scholar 

  • Dash, R. K. & Bassingthwaighte, J. B. Blood HbO2 and HbCO2 dissociation curves at varied O2, CO2, pH, 2,3-DPG and temperature levels. Ann Biomed Eng 32, 1676–1693 (2004).

    Article  Google Scholar 

  • Dash, R. K. & Bassingthwaighte, J. B. Simultaneous blood-tissue exchange of oxygen, carbon dioxide, bicarbonate and hydrogen ion. Ann Biomed Eng 34, 1129–1148 (2006).

    Article  Google Scholar 

  • Hickson, R. C. & Rosenkoetter, M. A. Separate turnover of cytochrome c and myoglobin in the red types of skeletal muscle. Am J Physiol 241, C140–C144 (1981).

    CAS  Article  Google Scholar 

  • Holloszy, J. O. & Booth, F. W. Biochemical adaptations to endurance exercise in muscle. Annu Rev Physiol 38, 273–291 (1976).

    CAS  Article  Google Scholar 

  • Yamada, T. et al. Interaction between myoglobin and mitochondria in rat skeletal muscle. J Appl Physiol 114, 490–497 (2013).

    CAS  Article  Google Scholar 

  • Box, G. E. P., Hunter, W. G. & Hunter, J. S. Statistics for Experimenters: An Introduction to Design, Data Analysis and Model Building. (John Wiley & Sons, New York, 1978).

  • Richardson, R. S. et al. Human skeletal muscle intracellular oxygenation: the impact of ambient oxygen availability. J Physiol 571, 415–424 (2006).

    CAS  Article  Google Scholar 

  • Schenkman, K. A., Marble, D. R., Burns, D. H. & Feigl, E. O. Myoglobin oxygen dissociation by multiwavelength spectroscopy. J Appl Physiol 82, 86–92 (1997).

    CAS  Article  Google Scholar 

  • Philip, L. A. & Dorothy, S. D. Respiration and Circulation. (Federation of American Societies for Experimental Biology, Bethesda, 1971).

  • Masuda, K. et al. Determination of myoglobin concentration in blood-perfused tissue. Eur J Appl Physiol 104, 41–48 (2008).

    CAS  Article  Google Scholar 

  • Srere, P. A. Citrate Synthase. Methods in Enzymology 13, 3–11 (1969).

    CAS  Article  Google Scholar 

Download references

Acknowledgements

This research was supported by a GRANT-in-Aid for Scientific Research from the Japanese Ministry of Education, Science, Sports and Culture (20680032, 21650167, KM; 22·6926, HT), with partial support from the Yamaha Motor Foundation for Sports (KM) and Nakatomi Foundation (KM). The authors appreciate Mr. Hideki Maeda (Hamamatsu Photonics, Shizuoka, Japan) for their great technical cooperation on this manuscript.

Author information

Authors and Affiliations

Authors

Contributions

H.T., K.M. and T.J. designed the research, H.T., Y.F., T.Y. and M.O. conducted the experiment and analysed the data, H.T. wrote the manuscript, K.M. and T.J. edited paper and T.H., S.I., T.H. and T.I. helped experiments.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Takakura, H., Furuichi, Y., Yamada, T. et al. Endurance training facilitates myoglobin desaturation during muscle contraction in rat skeletal muscle. Sci Rep 5, 9403 (2015). https://doi.org/10.1038/srep09403

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1038/srep09403

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing