Gene therapy has been investigated as a way to introduce a variety of genes to treat neurological disorders. An important clinical consideration is its long-term effectiveness. This research aims to study the long-term expression and effectiveness of gene therapy in promoting spiral ganglion neuron survival after deafness. Adenoviral vectors modified to express brain derived neurotrophic factor or neurotrophin-3 were unilaterally injected into the guinea pig cochlea one week post ototoxic deafening. After six months, persistence of gene expression and significantly greater neuronal survival in neurotrophin-treated cochleae compared to the contralateral cochleae were observed. The long-term gene expression observed indicates that gene therapy is potentially viable; however the degeneration of the transduced cells as a result of the original ototoxic insult may limit clinical effectiveness. With further research aimed at transducing stable cochlear cells, gene therapy may be an efficacious way to introduce neurotrophins to promote neuronal survival after hearing loss.
Conventional pharmalogical and surgical interventions are currently ineffective or unavailable for the treatment of a number of diseases within the central and peripheral nervous systems. As such, more novel approaches are currently being examined. One of these approaches is the use of gene therapy to restore function, prevent degeneration or even replace lost cells. Gene therapy has been utilised in a number of conditions at both the pre-clinical and clinical stage; including Parkinson's disease1,2, retinal blindness3,4 and hearing loss5,6,7,8. Gene therapy treatments for Parkinson's disease and various forms of retinal blindness have shown great promise, with transgene expression persisting for up to six years in a non-human primate model of Parkinson's disease9, and with many studies reaching phase II clinical trials. Whilst the use of gene therapy in animal models of hearing loss has also yielded positive results, there remain questions surrounding the longevity of gene expression within the cochlea, its long-term efficacy and its safety7,8,10.
The deaf cochlea provides a model to study gene therapy in the context of neural and tissue degeneration. After hearing loss induced by ototoxocity, for example, the sensory hair cells die, stimulating spiral ganglion neuron (SGN) degeneration and degeneration of the organ of Corti (OC). Gene therapy has been studied as a means to prevent the neural degeneration after hearing loss in order to preserve the neural elements required for cochlear implant use. Neurotrophin (NT) gene therapy that was targeted to the OC was shown to be more efficacious for preventing the degeneration of SGNs compared to gene expression that was expressed broadly throughout the cochlea7. However, the ototoxically-induced degeneration of the OC continued, even after early intervention with NT-gene therapy10,11. Despite this, gene expression has been observed for up to 11 weeks in the OC of the deafened cochlea with the NT gene expression resulting in SGN survival compared to contralateral cochleae10. However, from the time points thus far examined it could not be determined whether this degeneration would result in transduced cells undergoing apoptosis, thereby limiting gene expression and thus neurotrophic support for SGNs.
This paper will further elucidate the longevity and efficacy of adenovirus (Ad) which has been modified to express green fluorescent protein (GFP) in combination with either brain derived neurotrophic factor (BDNF) or neurotrophin-3 (NT3) (hereon in referred to as Ad-NTs) in order to help establish the suitability of NT gene therapy for maintaining SGNs after hearing loss. This will be achieved by examining the extent of OC degeneration, the longevity and pattern of gene expression, SGN density and peripheral fibre density, in ototoxically deafened guinea pig (GP) cochleae six months post-NT-gene therapy, a time point at which very few SGNs remain after deafness in the untreated GP12.
Viral gene expression profile
The GFP reporter gene present in Ad-NTs was used to examine the gene expression pattern within the ototoxically deafened cochlea six months post-injection. For the first time, these results show that viral vector expression is present for at least six months after inoculation in the deafened GP cochlea, with NT expression confirmed by co-localisation of GFP and BDNF. However, the overall GFP expression was significantly lower at 6 months compared to the shorter time-points examined in previous studies, as calculated by the density of GFP-positive pixels in mid-modiolar cochlear sections (p < 0.05, ANOVA; figure 1)7,10,13. Gene expression was predominately restricted to the basal turn of the cochlea, proximal to the site of injection. Gene expression beyond this area was observed in 1 GP only, in which GFP expression was observed in the lower middle turn of the cochlea. These results are consistent with previous studies7,8,10. The cells transduced within these regions included the pillar cells and Deiters' cells of the OC, cells of the spiral ligament and endosteal cells lining the endolymphatic and perilymphatic spaces. Transduced cell types in these GPs were consistent with those previously observed after 7 or 11 weeks of treatment (figure 1).
Concomitantly, when examined six months post-deafening there was minimal calretinin positive staining indicating a loss of HCs and degeneration of some supporting cells of the OC. Morphological analysis with H + E staining confirmed a flattening of the OC, which is a pathological response to ototoxic deafening14 (figure 2). There was symmetrical degeneration of the OC observed between the left treated cochleae and the right non-treated cochleae (figure 2).
Effects of long-term of neurotrophin gene therapy on SGN survival
To test if a single viral injection of Ad-NTs is able to provide long-term protection of SGNs after deafness, the density of SGN cell bodies was examined in the basal, middle and apical turns 6 months post NT-gene therapy treatment. These densities were then compared to the contralateral non-injected cochleae (figure 3).
There was a significantly greater density of SGNs in the basal turn of GPs treated with Ad-NTs when compared to the non-treated contralateral cochleae (895 ± 87 SGN/mm2 vs. 632 ± 74 SGN/mm2; p < 0.05, paired t-test), indicating that NT-gene therapy provided significant nerve survival in the basal region. There were no differences in SGN densities between treated cochleae and the contralateral cochleae in the middle turn (849 ± 182 SGN/mm2 vs. 596 ± 70 SGN/mm2; p > 0.05, paired t-test) or for the apical turn (533 ± 130 SGN/mm2 vs. 415 ± 89 SGN/mm2; p > 0.05, paired t-test). The overall survival observed was lower in this six month group compared to the 11 week data as denoted by the grey dashed lines on figure 3. In GPs treated with Ad modified to express GFP alone, there was no gain or loss of SGNs in cochleae injected with Ad-GFP compared to contralateral cochleae at 11 weeks post-injection10.
To determine the effect of NT-gene therapy on the peripheral fibres of SGNs in the deafened GP cochlea, the fibres were examined in cross-section of the OSL and compared to earlier time points. There was significant SGN peripheral fibre preservation in the NT-treated cochleae compared to the contralateral non-treated cochlea when examined after seven weeks of treatment (p < 0.001, paired t-test; figure 4), however, no significant difference was observed after either 11 weeks or six months of NT-gene therapy, indicating that long term NT-gene therapy is unable to prevent the degeneration of peripheral fibres.
Safety and viability of long-term gene therapy in the deaf cochlea
Similar to previous findings10, there was no evidence of cochlear infiltration by multinucleated giant cells or macrophages following Ad-delivery into the scala media, demonstrating that after an extended period in the cochlea adenoviral based gene therapy does not illicit an immune response. When examined after six months there was only one cochlea in which a tissue response was observed. The mild tissue response observed was composed of fibrous tissue and new bone growth, and was localised to the scala tympani of the lower basal turn (figure 5). The area of the scala tympani occupied by the tissue response was measured as 19.7% in this particular case and no response was observed in the scala media or in any of the other turns of the cochlea.
This study has demonstrated that Ad-mediated gene expression, as measured by both GFP expression and NT expression, is able to persist for up to six months in the cochlea post deafening, although gene expression was significantly reduced compared to earlier time points as demonstrated in figure 1. This is thought to be due to the continued degeneration of the OC that occurs after ototoxic injury8,10,14. Interestingly, NTs such as NT3, glial derived neurotrophic factor, and to a lesser extent BDNF, have been previously shown to play a protective role in the OC, when delivered prior to ototoxic insult15,16. These findings, along with the results of the current study, suggest that whilst it is possible to use NTs to provide protection to the OC in some conditions, it may not be possible to do so using NT gene therapy after a severe ototoxic insult.
The lower level of GFP expression and the continued degeneration of the OC suggest that expression may become further diminished to the point that it is no longer observed in the OC at periods greater than six months post-inoculation into the scala media of ototoxically deafened cochlea. Importantly, despite the lowered gene expression in the OC after six months there was still expression in other cochlear areas such as endosteal cells (4/5 of cochleae), interdental cells (2/5 of cochleae) and SL (2/5 of cochleae), indicating that Ad-mediated expression is able to persist long term as long as the transduced cells remain viable. Therefore, for long-term gene expression it is necessary to target cell populations within the cochlea that do not degenerate after hearing loss. When viewed in the context of lowered viral expression (especially within the OC) the results suggest that, whilst the long-term survival effect is maintained, SGNs are degenerating as a result of the lower gene expression in the six month cohort. As such, NT-gene therapy targeting the OC may not be a viable way to maintain SGNs in the ototoxically deafened GP long-term and, therefore other cell populations should be considered for transduction. One possible population could be the spiral ganglion Schwann cells, which could be targeted through the use of a Schwann cell-specific promoter such as PMP2217 and a surgical approach targeting this sub-population of cells.
When examined six months after inoculation, SGN survival was greater in the Ad-NTs treated cochlea compared to their contralateral cochlea, however the overall level of survival was decreased from earlier time points. This decrease in the level of survival correlated to a decrease in the level of gene expression. In the wider context though, gene therapy that is targeted to the OC should not be disregarded for several reasons. Firstly, the degeneration of the OC in the GP cochlea after ototoxic deafening progresses much faster than is observed in humans, and as such six months of deafness may equate to many years or even decades in humans18,19,20. Secondly, not all forms of deafness result in the complete degeneration of the OC (e.g. noise induced hearing loss) and therefore gene therapy targeting the OC in this form of deafness could be clinically viable. Thirdly, while various other methods have been used experimentally to introduce NTs into the deafened cochlea with varying degrees of success, their ability to provide long-term support in ototoxically deafened GP has yet to be shown21,22,23,24. Neurotrophin delivery using mini-osmotic pumps, for example, has been shown to be very effective during the treatment period, but this efficacy has not been shown to last more than two weeks after the cessation of treatment21,22. In contrast, gene therapy is a single-intervention technique that provides long-term exposure to neurotrophins and has the potential to maintain SGNs long-term after hearing loss.
The findings presented in this study extend upon previous work and confirm several key results which are critical in determining the viability of NT-gene therapy. These include the ability of Ad mediated gene expression to persist for an extended period in the deafened cochlea, the ability to provide long-term support for neurons and the ability to do this safely without causing a significant immune response in the cochlea. The minimal tissue response observed could be further mitigated in a number of ways including the administration of the glucocorticoid steroid dexamethasone, which has been shown to reduce the immune response associated with insertion of a cochlear implant into the cochlea25, or through the use of a soft surgical approach such as entering through the round window rather than performing a cochleostomy26. In clinical cases, accessing the scala media through a round window approach is possible, but not trivial, and would involve gaining access to the middle ear by entering the cochlea between the facial nerve and the chorda tympani.
Whilst there remain technical challenges in bringing viral gene therapy to the clinic, this study has highlighted that gene therapy that is targeted to the organ of Corti of the cochlea is ideal for targeting the sensory cells but has limitations relating to the degeneration of the sensory cells after severe hearing loss. In future studies, targeting NT-gene therapy to cell populations that do not undergo degeneration such as the spiral ganglion Schwann cells may enable longer-term expression and in turn promote greater survival of SGNs and their peripheral fibres.
Adenoviral vectors were generated as previously described7. Briefly, replication deficient adenovirus type 5 was genetically modified to expression GFP in concert with mouse BDNF or mouse NT3. Ad vectors were diluted 1:5 in artificial endolymph (120 mmol/l KCL, 2.5 mmol/l NaCl, 0.5 mmol/l MgCl2, 028 mmol/l CaCl2, 7.6 mmol/l K2HPO4, 2.7 mmol/l KH2PO4, pH 7.4) to final concentrations of 3.0 × 1010 OPU/ml (Ad-GFP-NT3) and 4.33 × 1010 OPU/ml (Ad-GFP-BDNF). Ad-GFP-NT3 and Ad-GFP-BDNF were mixed in a 1:1 ratio just prior to injection and will hereon be referred to as Ad-NTs.
Experimental animals and ethics
Male or female adult pigmented Dunkin-Hartley GPs (n = 5, average weight 323 ± 24.5 g) were used in this study. Viral administration was performed with the approval of the Office of the Gene Technology Regulator Australia (Licence #444). National Health and Medical Research Council of Australia and National Institutes of Health (NIH, USA) Guidelines for the Care and Use of Laboratory Animals were observed. The Animal Research Ethics Committee of the Royal Victorian Eye and Ear Hospital approved the care and use of the animals in this study.
The hearing status of each GP was assessed prior to deafening by measuring auditory brainstem responses (ABR) to computer-generated click stimuli27. For inclusion in the study GPs were required to have normal hearing, which was defined as an ABR threshold <43 dB peak-equivalent sound pressure level. Animals meeting this criterion were deafened under gaseous anaesthesia (Isoflurane; Abbott Laboratories, IL) via intravenous infusion of 100 mg/kg furosemide (Troy Laboratories, Smithfield, Australia) and subcutaneous 400 mg/kg kanamycin sulphate (Applichem, Taren Point, Australia)28. Deafness was confirmed one week post procedure using ABRs, where animals with a threshold shift of >50 dB were considered profoundly deaf.
Cochlea injection of viral sample
Two microliters of Ad-NTs, were unilaterally injected into the lower basal scala media of the cochlea one week post deafening as previously described7,10. This approach was selected to specifically target the OC for gene transfection.
Six months post injection GPs were deeply euthanised and intracardially perfused as previously described7. The bullae were removed and the cochleae exposed. Cochleae were decalcified, embedded in OCT and sectioned as previously described7,29. Half of the cochlea was retained for surface preparations as described7,10. Standard immunofluorescent protocols were followed using antibodies against heavy chain neurofilament (1:200, NF-H; Merck Millipore, Australia) to stain the SGNs and peripheral fibres, anti-calretinin (1:500, Merck Millipore, Australia) to stain cells within the OC, anti-BDNF (1:100, Santa Cruz Biotechnology, Santa Cruz, CA) to confirm NT production. AlexaFluor secondary antibodies (1:200–1:500, Molecular Probes, USA) were used to visualise several antibodies in the same sample and mounted in media containing DAPI. Sections were examined on a Zeiss Axioplan fluorescence microscope (Carl Zeiss, Germany). Cochlear half-turn surface preparations and pre mid-modiolar sections were viewed on a Zeiss Meta confocal microscope.
The level of GFP expression was analysed from three non-consecutive (greater than 72 μm apart) mid-modiolar sections from each cochlea. GFP-positive images were thresholded using ImageJ software (NIH, USA) and the pixel density of each image was measured. These measurements were averaged to obtain a single measurement for each animal. Statistical analysis of expression was performed using a one-way ANOVA and presented as mean ± SEM.
The extent of OC degeneration was quantified by measuring the area of the OC in the upper basal turn from three non-consecutive cochlear sections (greater than 72 μm apart) stained with Mayer's haemotoxylin and Putt's eosin mid-modiolar cross section using ImageJ software. The upper basal turn was used for quantification as it had high levels of adenoviral transduction without the confounding effect of the piercing through the basilar membrane to access the scala media, which was present in the lower basal turn.
SGN density was analysed blindly from three non-consecutive (greater than 72 μm apart) mid-modiolar sections from each cochlea. Density was calculated by counting co-labelled NF-H and DAPI-positive SGN cell bodies within Rosenthal's canal, and then dividing the number of SGNs by the area of Rosenthal's canal using ImageJ software. SGN densities in lower and upper basal, middle and apical turn were averaged to calculate the overall density of each cochlear region. Statistical analyses of SGN density data were performed using a paired student's t-test and presented as mean ± SEM.
Fibre density within the osseous spiral lamina
Fibre density within the osseous spiral lamina (OSL) was analysed blindly from three non-consecutive (greater than 72 μm apart) mid-modiolar haematoxylin and eosin (H + E) stained sections from each cochlea. This was calculated by inverting the images and tracing the OSL to ascertain the mean grey value within this region, using ImageJ software. Statistical analyses of peripheral fibre density data were performed using a paired t-test and presented as mean ± SEM.
The chronic tissue response to the surgery and viral inoculation was assessed by measuring the area of tissue response in mid-modiolar cross sections and calculating that as a percentage of the total area of the scala tympani. Tissue response was quantified in H + E sections in three non-consecutive cochlear sections (greater than 72 μm apart) using a Zeiss Axio Imager M2 microscope and analysed using Image J.
The authors would like to acknowledge and thank Assistant professor Clifford Hume from the University of Washington for the adenovirus used in this paper. The authors would also like to acknowledge the funding support from The Garnett Passe and Rodney Williams Memorial Foundation, Action on Hearing Loss, The University of Melbourne Department of Otolaryngology, The Royal Victorian Eye and Ear Hospital, National Health and Medical Research Council (GNT1024350). The authors would like to acknowledge the support from the State Government of Victoria's Operational Infrastructure Support Program.
About this article
Gene Therapy (2016)