Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Effects of Bio-Au Nanoparticles on Electrochemical Activity of Shewanella oneidensis Wild Type and ΔomcA/mtrC Mutant

Abstract

Both Shewanella oneidensis MR-1 wild type and its mutant ΔomcA/mtrC are capable of transforming AuIII into Au nanoparticles (AuNPs). Cyclic voltammetry reveals a decrease in redox current after the wild type is exposed to AuIII but an increase in oxidation current for the mutant. The peak current of the wild type is much higher than that of the mutant before the exposure of AuIII, but lower than that of the mutant after the formation of AuNPs. This suggests that damage to the electron transfer chain in the mutant could be repaired by AuNPs to a certain extent. Spectroscopy and SDS-PAGE analysis indicate a decrease in cell protein content after the formation of AuNPs, which provides a convenient way to detect intracellular information on cells.

Introduction

Metal nanoparticles have been widely investigated due to their unique properties in optics, chemistry, electronics and magnetics. Biosynthesis of metal nanoparticles, as a reliable and eco-friendly method, has led to increasing interest in their synthetic mechanisms and applications. Many bacteria have been identified to be able to reduce metals, such as Shewanella oneidensis1,2,3, Geobacter sulfurreducens4,5, Desulfovibrio desulfuricans6,7,8, Aeromonas hydrophila9, and Pantoea agglomerans10. Bio-reduced metal nanoparticles have potential uses in biotechnological and environmental processes11. For example, biosynthesized Pd nanoparticles were found to be able to exhibit a high catalytic activity for lactate oxidation and molecular hydrogen production8. Bio-reduced Au–Pd nanoparticles showed a comparable catalytic activity to chemosynthetic counterparts in regard to dechlorination of diclofenac and the oxidation of benzyl alcohol12,13.

Shewanella oneidensis, a dissimilatory metal-reducing microorganism, has the ability to reduce metal oxides through their respiration14,15,16. The process of metal reduction is associated with extracellular electron transfer. One proposed mechanism is Mtr (i.e. metal-reducing) respiratory pathway, which is considered to be a vital anaerobic respiratory pathway17. In such a process, a series of c-type cytochromes transfer electrons from inner membrane via periplasm to outer membrane17,18, electrons eventually arriving at metal oxide directly or mediated by electroactive metabolites/secretions19. Outer membrane cytochromes (OMCs), OmcA and MtrC, located on the surface of the outer membrane are key proteins transferring electrons to external electron acceptor in the Mtr pathway. However, it is not clear whether the two proteins are responsible for metal reduction. Besides, although there have been some studies on the microbial process of transforming AuIII into AuNPs20,21, little is known about the role of AuNPs in bacterial electron transfer, biochemical properties and physical functions.

When metal nanoparticles are present in cells, it will cause the variation of cell composition and function. Spectroscopy is a sensitive way that can be used to infer structural molecular information or composition from a sample. Surface Enhanced Raman Spectroscopy (SERS), which usually makes use of colloidal Au/Ag particles, has been widely applied to study bacterial compositions22,23,24,25. Although the intracellular AuNPs point to a more promising way in understanding cellular processes, there have been only a few studies of acquired SERS spectra from internalized colloids26,27. X-ray photoelectron spectroscopy (XPS)28, over a depth of about 5 nm, has been used to study the surface chemical composition of a variety of microbial cells, involved in surface properties and adhesion behaviors29,30. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) assay is a visualized and effective method to further study the variation of cell total protein after AuIII reduction.

In this work, S. oneidensis MR-1 (wild-type and ΔomcA/mtrC) were used to synthesize AuNPs. We not only proposed the mechanisms of AuIII bio-reduction, but also investigated the roles of AuNPs in biochemical properties and physical functions by imaging, electrochemistry, spectroscopy and gel electrophoresis analysis.

Results

Characterization of biosynthetic AuNPs

Natural S. oneidensis MR-1 cells were rod-shaped with a relatively smooth surface (Fig. 1a). During exposure to an aqueous HAuCl4 solution (100 mg L−1 AuIII), the color of the cell suspension changed from pale yellow to purple (see Supplementary Fig. S1 online) within 30 min. A control experiment with only lactate and HAuCl4 aqueous solution under anaerobic condition showed no color change or precipitation. The distinct color change in the bacterial experiment provides a visible signature for the formation of AuNPs31. SEM images showed irregular-shaped spots on the bacterial cell surface after the wild type is exposed to AuIII for 1 h (Fig. 1b) and the size distribution is displayed in Fig. 1c. EDX spectroscopy confirmed that these spots were elemental AuNPs (Fig. 1d). Further analysis by TEM reveals that AuNPs were embedded in the membrane (Fig. 1e). The mutant lacking MtrC and OmcA proteins could still synthesize AuNPs in 30 min. Fig. 1f shows the location of biosynthesized AuNPs in a mutant cell.

Figure 1
figure 1

Characteristics of biosynthetic AuNPs.

SEM images of (a) a natural wild type cell and (b) a Au-wild type cell; (c) The distribution in size of bio-synthesized AuNPs; (d) EDX spectrum of Au-wild type cells; TEM images of (e) Au-wild type and (f) Au-mutant cells.

Electrochemical properties

Mediated electron transfer could be excluded since the cell pellets were washed to remove residual compounds. Curve 1 in Fig. 2a shows a direct electron transfer between the bacteria and electrode when the wild type was coated onto a glassy carbon surface. Oxidation peaks appeared at −0.28 and +0.06 V and a reduction peak at −0.25 V. Cyclic voltammetry results also reveal that ΔomcA/mtrC was still capable of reversible electron transfer, although the peak currents and integrated areas were much lower compared to the wild type32,33 (Fig. 2b Curve 1).

Figure 2
figure 2

Cyclic voltammograms of S. oneidensis MR-1 before and after the formation of AuNPs.

(a) (1) Au-free wild-type coated on a GC electrode; Au-wild type coated onto a GC electrode in the (2) absence and (3) presence of lactate. (b) (1) Au-free ΔomcA/mtrC and (2) Au-ΔomcA/mtrC coated on a GC electrode.

To explore the roles of the biosynthetic AuNPs in electrochemical characteristics of the strain, S. oneidensis MR-1 was exposed to AuIII (10 mg L−1) for 1 h. Large changes could be observed between the Au-free wild type and the Au-wild type cells in Fig. 2a. Redox current was reduced after the wild type synthesized AuNPs. Although the current decreased in magnitude redox peaks were still observed. A comparison between Curve 1 and Curve 2 in Fig. 2a shows a new reduction wave at a potential of −0.5 V, but this did not show in the CV for the raw strain. Control experiments were conducted in anaerobic (in the absence of electron acceptor) and aerobic culture conditions. In the two situations, the peak at −0.5 V was not observed. Considering that the reduction potential of Au is not at −0.5 V and no hydrogen desorption reaction occurs (see Supplementary Fig. S2 online), the influence of reduced Au is also excluded. Hence, the emerging peak may be attributed to the proteins on the bacterial membrane transferring electrons to an exogenous electron acceptor, rather than oxygen, in their metabolism. The addition of lactate leads to an oxidation current response after the wild type synthesis of AuNPs (Curve 3 in Fig. 2a), which means the strain is still capable of oxidizing organics.

In order to investigate whether Mtr pathway was responsible for AuIII reduction, ΔomcA/mtrC mutant was used in this study. However, different from the wild type, the mutant showed an increase in oxidation currents after the reduction of AuIII (Fig. 2b). The comparison of redox current between the two strains before and after synthesizing AuNPs is shown in Table 1,which is obtained by 10 replicate cyclic voltammetry tests. Although the Au-free ΔomcA/mtrC revealed a lower electrochemical activity compared with the Au-free wild type, the redox peaks of the Au-ΔomcA/mtrC were higher than those of the Au-wild type.

Table 1 Summary of peak current data in cyclic voltammetry of the wild-type and the mutant before and after the exposure to AuIII

Microorganisms obtain electrons from oxidized substrates through their respiratory processes. The addition of lactate leads to an oxidation current response in the Au-wild type and the Au-mutant. A further investigation of potentiostatic test on Au-cells revealed a clear lactate-oxidation current at 50 s after the addition of lactate (see Supplementary Fig. S3 online). In the benchmark experiment in which Au-free cells were tested, no obvious peak current change could be observed in 50 s or in even at slightly longer time. However, previous work revealed an increasing current in cyclic voltammetry of 30 min after the addition of lactate32. Therefore, the process for lactate oxidation through anaerobic respiration of S. oneidensis was considered to be a slow reaction. Supplementary Fig. S3 shows that after S. oneidensis transformed AuIII, this process was accelerated (current rapidly increased about 10 nA after 50 s). This indicates that the bio-synthetic AuNPs help S. oneidensis transfer electrons to oxidize lactate. In the control experiment of AuNPs electrodeposited on an identical GC electrode surface, no oxidation current increase was detected in potentiostatic tests after the addition of lactate. The result illustrates that AuNPs themselves could not catalyze lactate oxidation, but electron transfer would be facilitated when they combined with the cell membrane.

Spectroscopy characteristics

Raman and SERS analysis are used to detect changes of bacterial compositions after S. oneidensis transformed AuIII into AuNPs. No distinct peaks could be observed for the AuNPs-free control samples of the two strains. It means that little chemical information was presented in the absence of AuNPs. After the addition of chemosynthetic AuNPs or the formation of bio-reduced AuNPs, signals were sufficiently enhanced. Representative bands of proteins, nucleic acids and lipids could be observed in Fig. 3. The major bands assignments have been shown in Table 2. The sharpest and stable SERS band at ~736 cm−1 is ubiquitous in bacteria and has been assigned to adenosine in previous studies34. Therefore we take the ~736 cm−1 band as a reference in the following analysis. The amide III region is represented by bands at 1230 ~ 1245 cm−1, but neither amide I nor amide II bands were observed in the spectrum. This perhaps illustrates that SERS revealed the enhancement of key proteins characteristics in spectroscopy and these proteins are more likely to be associated with membrane involved in the reduction of AuIII26. No obvious difference can be detected in spectra between the wild type and the mutant.

Table 2 Tentative band assignments for SERS acquired from S. oneidensis MR-1 wild type with biosynthetic and chemosynthetic AuNPs
Figure 3
figure 3

SERS of S. oneidensis MR-1 wild type.

In the presence of (a) chemosynthetic; and (b) biosynthetic AuNPs substrate.

When comparing the SERS spectra obtained from the biosynthetic and chemosynthetic AuNPs labelled samples, slight differences could be detected. The different intensities of the bands at 1235–1245 and 1455 cm−1 relative to the sharpest and stable band at ~736 cm−1 suggest a varying contribution from proteins (amide III) and lipids. The biosynthetic AuNPs labelled sample shows lower intensities at the two bands, that is that a decline in protein quantities or protein structure change has happened in the AuNPs formation process. The variation in amide III content was further confirmed by Fourier transform infrared spectroscopy (FTIR). Detailed information is available in Supplementary Fig. S4 online.

The surface chemical composition of bacteria was further studied by XPS. Table 3 displays mole fraction and atomic mass ratios of main elements with respect to total carbon. Duplicate experiments showed similar data. Notably, N quality which mainly contributed to amine or amide varied a lot. Strains before AuNPs formation are much richer in N content than Au-strains. The variation in N content is also reflected by Fig. 4 and binding energy of N appeared at around 400.0 eV. The main constituents of bacterial cell surface are polysaccharides (Ps), proteins (Pr) and lipids (Lp). According to previous studies29,35, C6H10O5 represents for polysaccharides and CH2 for lipids in chemical composition model. The molecular compositions were calculated by the following formulas:

Table 3 Cell surface chemical composition and deduced proportions of carbon referred to proteins, polysaccharides and lipids
Figure 4
figure 4

Cell surface chemical composition.

Representative N1s spectra obtained for the wild type, Au-wild type, mutant and Au-mutant.

Comparing with the results showed in Table 3, significant differences in protein content were observed. The wild type and mutant are much richer in protein than Au-wild type and Au-mutant, which verifies SERS and FTIR results. Besides, more significant decrease in Au-wild type was observed.

Protein analysis by SDS-PAGE

Total protein contents of both the wild type and the mutant are significantly decreased after the formation of AuNPs (Fig. 5). Notably, the decrease in wild type protein content is much larger. In order to obtain the precise protein variation caused by the biosynthesis of AuNPs, all samples were electrophoresed at the same protein content. The effects of biosynthesis of AuNPs on total cell protein of the wild type and the mutant are shown in Fig. 6. About 35 obvious bands in the gel of the total proteins prepared from the two strains before and after the exposure of AuIII and separated by SDS-PAGE could be observed. The protein band patterns of these four samples were similar to each other, but variations in the degree of brightness could still be detected. After the exposure of AuIII, the bacterial total protein content was dramaticlly modified at both the wild type and the mutant. Many protein bands from AuIII exposed cells were not as distinct as in the unexposed ones, especially in below 35 kDa area. Protein extracts from both Au-wild type and Au-mutant showed 8 proteins (about 80 kDa, 66 kDa, 30 kDa, 26 kDa, 21 kDa, 19 kDa) that were significantly reduced in content compared with the unexposed ones. In addition, the total protein content of Au-wild type was more drastically diminished on the gel than the Au-mutant in the < 25 kDa region. That is, small molecular protein concentrations in Au-wild type were significantly lower than that in the Au-mutant.

Figure 5
figure 5

Total protein content.

Variations in total cell protein contents before and after the formation of AuNPs by both strains.

Figure 6
figure 6

Protein analysis.

SDS-PAGE analysis of total protein from the two strains before and after the formation of AuNPs.

Discussion

It is found that the mutant could still transform AuIII into AuNPs (Fig. 1f) and the color change time in suspension and characteristics (size, shape and location) of bio-AuNPs are basically the same as the wild type. This discovery demonstrates that lack of OmcA and MtrC proteins does not affect the pathway or the location of AuNPs formation, i.e. OmcA and MtrC proteins are not necessary in the AuIII reduction process.

Since the bio-reduced AuNPs are embedded in membrane judged by TEM images, there are two possible explanations. One is that AuIII ions could get into bacterial outer membrane and be reduced. In Mtr pathway, besides OmcA and MtrC exposed on the outer surface of the outer membrane, there are MtrA facing interior and MtrB functions as a transmembrane sheath36,37 which envelops MtrA and MtrC. Both MtrA and MtrC are partially inserted in MtrB and MtrC are bounded with OmcA38. A previous study on FeIII reduction by MtrCAB proteoliposomes has demonstrated that in the presence of soluble FeIII, limited electron transport via MtrAB still occurs39. However, electrons cannot be transferred to insoluble FeIII in the absence of OmcA and MtrC39. This means the two proteins are more important in transferring electrons to insoluble electron acceptors. Since the porin-like outer membrane protein MtrB was near the membrane surface, soluble ions would be capable of penetrating far enough into the MtrB to make electrical contact with MtrA39,40. Lacking of OmcA and MtrC proteins, AuIII ions could easily get into MtrB porin and accept electrons transported by MtrA, thus formed the AuNPs. Another possible explanation is that electrons could be transferred from bacteria to AuIII ions through other protein functional groups located on the outer membrane. Apart from Mtr pathway, electrons may also be transported across the bacterial outer membrane by other protein conduits composed of MtrE and DmsF (i.e. dimethyl sulphoxide F), a homologue of MtrB41. However, whether electrons are transferred through this pathway during AuIII reduction needs further study. In the both situations, the formation of AuNPs would not be affected when S. oneidensis cells lack the OmcA and MtrC proteins. That is, electrons can be transferred to AuIII ions by OmcA and MtrC when the two proteins exist and by other proteins/pathways when they are absent.

Considering the increase in redox current of the Au-ΔomcA/mtrC, this demonstrates that a low loading AuNPs may help bacteria transfer electrons when lacking the OmcA and MtrC proteins. Damage to the electron transfer chain in mutant could be repaired to a certain extent after the formation of bio-AuNPs so that the mutant even exhibited a better electrochemical activity than the wild type. Hence, an approach to repairing cell electron transfer damage is proposed; this new discovery may have a promising future in biology and biochemistry fields.

SERS and FTIR studies explain the decrease in current of cyclic voltammogram after the wild type reduced AuIII. The variation of amide III intensity is probably because the AuNPs formation and growth, which would probably cause damage to the cell wall structure8 and so lead to a decline in protein quantities or protein structure change. As a result, accessible electroactive centres would decrease in number. Although a protein quantity decrease could also be observed in SERS and FTIR of biosynthetic Au-ΔomcA/mtrC, oxidation currents of the mutant were increased (Table 1). This discovery illustrates that the proteins affected by AuNPs are involved in electron transfer of the wild type but not in that of the mutant. It means the proteins which lead to a decrease in amide III are very likely part of the Mtr pathway, since only the Mtr pathway had been cut off in the mutant.

Protein SDS-PAGE was conducted to further confirm the validity of spectroscopy results. In order to detect the precise protein variation, each strain was cultivated in one bottle before AuIII addition and the protein load of each sample was 10 μg. From Fig. 6, we can see the brightness of 8 bands (about 80 kDa, 66 kDa, 30 kDa, 26 kDa, 21 kDa, 19 kDa) was distinctly decreased after the exposure of AuIII. The result indicates that some proteins would be inhibitted or damaged by the formation of AuNPs; this conclusion is in accordance with the results from spectroscopy. Notebly, at < 25 kDa, the decrease of protein concentration of the Au-wild type were more remarkable than that of Au-mutant. This reveals that small molecular proteins of the wild type would more easily be affected and that may be why the current of the wild type in cyclic voltammetry was dramaticlly decreased.

In conclusion, we report that both S. oneidensis MR-1 wild type and its mutant ΔomcA/mtrC are able to transform AuIII into AuNPs. The outer membrane cytochromes OmcA and MtrC are not necessary in AuIII reduction. The bio-precipitated AuNPs might be able to participate in electron transfer, catalyze the oxidation of organics and repair cell damage in electron transfer to some extent. SERS and FTIR spectra obtained from the biosynthetic AuNPs labelled cells revealed a decrease in protein content and provided a reliable and convenient way to detect intracellular information of cells. Notably, due to the moderate reaction conditions and rapid reaction time, this is a promising way to obtain SERS in bacteria. Total protein SDS-PAGE assay further investigated the molecular weight of the decreased proteins. The present study aids better understanding of the effect of biosynthesized AuNPs on the electrochemical activities of the wild type and the mutant.

Methods

Cultivation and AuIII reduction

Strains were grown aerobically at 32°C in 50 mL lactate contained Luria-Bertani medium overnight. The cells were collected by centrifugation at 6000 rpm for 5 min and were washed three times by sterile distilled water. The washed pellets were re-suspended in 50 mL sterile distilled water with lactate (50 mM) as the electron donor in 100 mL serum bottles. HAuCl4·4H2O was added to a final Au concentration of 10 mg L−1. Anaerobic conditions were maintained by bubbling nitrogen and then the serum vials were sealed. The cell-resuspened solutions were cultivated at 32°C as before.

Scanning electron microscopy (SEM) and transmission electron microscopy (TEM)

Cells were fixed in 2.5% (w/v) glutaraldehyde for 4 h. After washing three times with phosphate buffer (50 mM, pH 7.0), the cells were dehydrated in an ethanol series (50%, 70%, 90% and 100% twice). After dring at 80°C for 12 h, the cells were placed onto a carbon substrate. SEM and EDX images were carried out by a Hitachi S-4800 Scanning electron microscope with an EDX attachment at 5 kV.

Cells were prefixed in 2.5% (w/v) glutaraldehyde for 4 h and fixed in 2% osmium tetraoxide for 4 h. Then the cells were washed by phosphate buffer (50 mM, pH 7.0), dehydrated by an ethanol series (50%, 70%, 80%, 90% and 100% twice) and embedded in resin (LR White). The mixtures were polymerized at 60°C for 24 h. Ultrathin sections of ~60 nm were cut by an ultramicrotome and deposited on carbon-coated copper grids. A Hitachi H-7650 transmission electron microscope was used to acquire TEM images at 80 kV.

Electrode preparation and electrochemical measurements

After exposed to AuIII for 1 h, cells were collected by centrifugation at 6000 rpm for 5 min and washed three times with phosphate buffer (50 mM, pH 7.0). 5 μL cells were transferred to the surface of a glassy carbon (GC) electrode by pipette and dried naturally at room temperature.

A three-electrode chamber with the prepared GC working electrode, an Ag/AgCl reference electrode and a Pt wire counter electrode was used to measure electrochemical characteristics in a Faraday cage to exclude interferences. Cyclic voltammetry and potentiostatic measurements were carried out by an Autolab electrochemical workstation. The autolab is equipped with ECD module which can amplify low current and makes the detection limit 100 pA. The scan rate for cyclic voltammetry was 10 mV/s and we chose the eighth of cycle run to make comparisons. All electrochemical tests were reduplicated at least 5 times. Phosphate buffer (50 mM, pH 7.0) was used as electrolyte. All experiments were conducted under a nitrogen atmosphere.

Raman and SERS

Cells were collected by centrifugation at 6000 rpm for 5 min, washed three times by sterile distilled water and then the pellet was dropped on a clean glass slide. Raman and SERS spectra of S. oneidensis were recorded on a Horiba Jobin Yvon S.A.S. LabRAM Aramis Laser confocal Microscope Raman spectrometer, with a 632.8 nm HeNe lase yielded ~7.25 mW power at the sample and the exposure time of each experiment was 1–10 s. Chemosynthetic AuNPs were prepared in accordance with Frens' method42. In short, 100 mL of 0.01% (w/v) HAuCl4 solution was heated to boiling under sufficient stirring, 0.6 mL of 1% trisodium citrate was then immediatly added. The mixture was kept boiling for 1 h.

X-ray photoelectron spectroscopy (XPS)

The cells were dried by freeze drying method. XPS analyses were carried out by a Thermo Escalab 250 electron energy disperse spectrometer. The angle between the electrostatic lens axis and the sample surface was 0°. C1s spectrum was used as reference to obtain the binding energies of all spectra.

Total cell protein content assay

To ensure that bacteria before and after AuNPs formation have same cell concentrations and growth conditions, each strain was cultivated in one bottle. The cells were collected by centrifugation of 200 mL bacterial solution at 6000 rpm for 5 min and washed 3 times by sterile distilled water. The cell pellets were then resuspended in 3 mL NaOH liquid solution (1 M) and heated in boiling water for 30 min. Total cell proteins were obtained in the supernatant after the heated resuspension was centrifuged at 10000 rpm for 20 min. Total protein contents were measured by Bradford Assay.

SDS-PAGE

The cell total proteins were extracted by grinding bacteria in a mortar with liquid nitrogen. Then the disrupted cells were collected by resolving in PBS (50 mM, pH 7.0) and total proteins were obtained from the supernatant by centrifugation at 12000 rpm for 20 min at 4°C. Protein extracts were analyzed using 15% polyacrylamide resolving gels and 5% polyacrylamide stack gels. The protein loading quantity of each sample was 10 μg. All samples were bathed in boiling water for 5 min and electrophoresed with constant voltage until the dye front reached the bottom of the gel. The protein bands were visualized by Coomassie Brilliant Blue G-250 staining, methanol and acetic acid destaining.

References

  • Myers, C. R. & Nealson, K. H. Bacterial manganese reduction and growth with manganese oxide as the sole electron acceptor. Science 240, 1319–1321 (1988).

    ADS  CAS  PubMed  Article  Google Scholar 

  • Viamajala, S., Peyton, B. M., Apel, W. A. & Petersen, J. N. Chromate reduction in Shewanella oneidensis MR-1 is an inducible process associated with anaerobic growth. Biotechnol. Prog. 18, 290–295 (2002).

    CAS  PubMed  Article  Google Scholar 

  • Ng, C. K. et al. Influence of outer membrane c-type cytochromes on particle size and activity of extracellular nanoparticles produced by Shewanella oneidensis. Biotechnol. Bioeng. 110, 1831–1837 (2013).

    CAS  PubMed  Article  Google Scholar 

  • Lovley, D. R. et al. Geobacter metallireducens gen. nov. sp. nov., a microorganism capable of coupling the complete oxidation of organic compounds to the reduction of iron and other metals. Arch. Microbiol. 159, 336–344 (1993).

    CAS  PubMed  Article  Google Scholar 

  • Aklujkar, M. et al. Proteins involved in electron transfer to Fe(III) and Mn(IV) oxides by Geobacter sulfurreducens and Geobacter uraniireducens. Microbiology 159, 515–535 (2013).

    CAS  PubMed  Article  Google Scholar 

  • Lloyd, J. R., Yong, P. & Macaskie, L. E. Enzymatic recovery of elemental palladium by using sulfate-reducing bacteria. Appl. Environ. Microbiol. 64, 4607–4609 (1998).

    CAS  PubMed  PubMed Central  Google Scholar 

  • Deplanche, K. & Macaskie, L. E. Biorecovery of gold by Escherichia coli and Desulfovibrio desulfuricans. Biotechnol. Bioeng. 99, 1055–1064 (2008).

    CAS  PubMed  Article  Google Scholar 

  • Wu, X. et al. A role for microbial palladium nanoparticles in extracellular electron transfer. Angew. Chem. Int. Ed. 50, 427–430 (2011).

    CAS  Article  Google Scholar 

  • Wu, Y., Li, Y., Liu, P., Gardner, S. & Ong, B. S. Studies of gold nanoparticles as precursors to printed conductive features for thin-film transistors. Chem. Mater. 18, 4627–4632 (2006).

    CAS  Article  Google Scholar 

  • Huang, X., Jain, P. K., El-Sayed, I. H. & El-Sayed, M. A. Gold nanoparticles: interesting optical properties and recent applications in cancer diagnostic and therapy. Nanomedicine 2, 681–693 (2007).

    CAS  PubMed  Article  Google Scholar 

  • Lloyd, J. R., Lovley, D. R. & Macaskie, L. E. Biotechnological application of metal-reducing microorganisms. Adv. Appl. Microbiol. 53, 85–128 (2003).

    CAS  PubMed  Article  Google Scholar 

  • De Corte, S. et al. Doping of biogenic Pd catalysts with Au enables dechlorination of diclofenac at environmental conditions. Water Res. 46, 2718–2726 (2012).

    CAS  PubMed  Article  Google Scholar 

  • Deplanche, K. et al. Microbial synthesis of core/shell gold/palladium nanoparticles for applications in green chemistry. J. R. Soc. Interface 9, 1705–1712 (2012).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  • Beliaev, A. S. & Saffarini, D. A. Shewanella putrefaciens mtrB encodes an outer membrane protein required for Fe(III) and Mn(IV) reduction. J. Bacteriol. 180, 6292–6297 (1998).

    CAS  PubMed  PubMed Central  Google Scholar 

  • Kathiresan, K., Manivannan, S., Nabeel, M. A. & Dhivya, B. Studies on silver nanoparticles synthesized by a marine fungus, Penicillium fellutanum isolated from coastal mangrove sediment. Colloids Surf. B Biointerfaces 71, 133–137 (2009).

    CAS  PubMed  Article  Google Scholar 

  • Jiang, S. et al. Bacterial formation of extracellular U(VI) nanowires. Chem. Commun. 47, 8076–8078 (2011).

    CAS  Article  Google Scholar 

  • Shi, L., Squier, T. C., Zachara, J. M. & Fredrickson, J. K. Respiration of metal (hydr)oxides by Shewanella and Geobacter: a key role for multihaem c-type cytochromes. Mol. Microbiol. 65, 12–20 (2007).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  • Shi, L. A. et al. The roles of outer membrane cytochromes of Shewanella and Geobacter in extracellular electron transfer. Env. Microbio.l Rep 1, 220–227 (2009).

    CAS  Article  Google Scholar 

  • Marsili, E. et al. Shewanella secretes flavins that mediate extracellular electron transfer. Proc. Natl. Acad. Sci. USA 105, 3968–3973 (2008).

    ADS  CAS  PubMed  Article  PubMed Central  Google Scholar 

  • Konishi, Y. et al. Intracellular recovery of gold by microbial reduction of AuCl4 ions using the anaerobic bacterium Shewanella algae. Hydrometallurgy 81, 24–29 (2005).

    Article  CAS  Google Scholar 

  • De Corte, S. et al. Gold nanoparticle formation using Shewanella oneidensis: a fast biosorption and slow reduction process. J. Chem. Technol. Biotechnol. 86, 547–553 (2011).

    CAS  Article  Google Scholar 

  • Jarvis, R. M., Brooker, A. & Goodacre, R. Surface-enhanced Raman spectroscopy for bacterial discrimination utilizing a scanning electron microscope with a Raman spectroscopy interface. Anal. Chem. 76, 5198–5202 (2004).

    CAS  PubMed  Article  Google Scholar 

  • Jarvis, R. M. & Goodacre, R. Discrimination of bacteria using surface-enhanced Raman spectroscopy. Anal. Chem. 76, 40–47 (2004).

    CAS  PubMed  Article  Google Scholar 

  • Jarvis, R. M., Brooker, A. & Goodacre, R. Surface-enhanced Raman scattering for the rapid discrimination of bacteria. Faraday Discuss. 132, 281–292 (2006).

    ADS  CAS  PubMed  Article  Google Scholar 

  • Premasiri, W. R. et al. Characterization of the Surface Enhanced Raman Scattering (SERS) of bacteria. J. Phys. Chem. B 109, 312–320 (2005).

    CAS  PubMed  Article  Google Scholar 

  • Jarvis, R. M. et al. Surface-enhanced Raman scattering from intracellular and extracellular bacterial locations. Anal. Chem. 80, 6741–6746 (2008).

    CAS  PubMed  Article  Google Scholar 

  • Ravindranath, S. P., Henne, K. L., Thompson, D. K. & Irudayaraj, J. Surface-Enhanced Raman Imaging of Intracellular Bioreduction of Chromate in Shewanella oneidensis. Plos One 6, 1–10 (2011).

    Article  CAS  Google Scholar 

  • McArthur, S. L. Applications of XPS in bioengineering. Surf. interface anal. 38, 1380–1385 (2006).

    CAS  Article  Google Scholar 

  • Rouxhet, P. et al. Application of X-ray photoelectron spectroscopy to microorganisms. Colloids Surf. B 2, 347–369 (1994).

    CAS  Article  Google Scholar 

  • van der Mei, H. C., De Vries, J. & Busscher, H. J. X-ray photoelectron spectroscopy for the study of microbial cell surfaces. Surf. sci. rep. 39, 1–24 (2000).

    ADS  CAS  Article  Google Scholar 

  • Ruscher, C. H. & Speer, D. The color of nanodispersed gold, silver and gold-silver solid solutions in class matrices. CFI-Ceram. Forum Int. 78, E41–E45 (2001).

    CAS  Google Scholar 

  • Baron, D., LaBelle, E., Coursolle, D., Gralnick, J. A. & Bond, D. R. Electrochemical Measurement of Electron Transfer Kinetics by Shewanella oneidensis MR-1. J. Biol. Chem. 284, 28865–28873 (2009).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  • Okamoto, A., Nakamura, R. & Hashimoto, K. In-vivo identification of direct electron transfer from Shewanella oneidensis MR-1 to electrodes via outer-membrane OmcA-MtrCAB protein complexes. Electrochim. Acta 56, 5526–5531 (2011).

    CAS  Article  Google Scholar 

  • Chan, J. W. et al. Monitoring dynamic protein expression in living E-coli. Bacterial Celts by laser tweezers raman spectroscopy. Cytometry Part A 71A, 468–474 (2007).

    CAS  Article  Google Scholar 

  • Dague, E., Delcorte, A., Latge, J.-P. & Dufrene, Y. F. Combined use of atomic force microscopy, X-ray photoelectron spectroscopy and secondary ion mass spectrometry for cell surface analysis. Langmuir 24, 2955–2959 (2008).

    CAS  PubMed  Article  Google Scholar 

  • Ross, D. E. et al. Characterization of protein-protein interactions involved in iron reduction by Shewanella oneidensis MR-1. Appl. Environ. Microbiol. 73, 5797–5808 (2007).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  • Hartshorne, R. S. et al. Characterization of an electron conduit between bacteria and the extracellular environment. Proc. Natl. Acad. Sci. USA 106, 22169–22174 (2009).

    ADS  CAS  PubMed  Article  PubMed Central  Google Scholar 

  • Cai, P. J. et al. Anaerobic biodecolorization mechanism of methyl orange by Shewanella oneidensis MR-1. Appl. Microbiol. Biotechnol. 93, 1769–1776 (2012).

    CAS  PubMed  Article  Google Scholar 

  • White, G. F. et al. Rapid electron exchange between surface-exposed bacterial cytochromes and Fe(III) minerals. Proc. Natl. Acad. Sci. USA 110, 6346–6351 (2013).

    ADS  CAS  PubMed  Article  PubMed Central  Google Scholar 

  • Shi, L., Rosso, K. M., Zachara, J. M. & Fredrickson, J. K. Mtr extracellular electron-transfer pathways in Fe(III)-reducing or Fe(II)-oxidizing bacteria: a genomic perspective. Biochem. Soc. Trans. 40, 1261–1267 (2012).

    CAS  PubMed  Article  Google Scholar 

  • Coursolle, D. & Gralnick, J. A. Modularity of the Mtr respiratory pathway of Shewanella oneidensis strain MR-1. Mol. Microbiol. 77, 995–1008 (2010).

    CAS  PubMed  Google Scholar 

  • Frens, G. Controlled Nucleation for Regulation of Particle-size in Monodisperse Gold Suspensions. Nature-Physical Science 241, 20–22 (1973).

    ADS  CAS  Article  Google Scholar 

  • Liu, Y. et al. Antibacterial activities of zinc oxide nanoparticles against Escherichia coli O157:H7. J. Appl. Microbiol. 107, 1193–1201 (2009).

    CAS  PubMed  Article  Google Scholar 

  • Yang, X., Gu, C., Qian, F., Li, Y. & Zhang, J. Z. Highly Sensitive Detection of Proteins and Bacteria in Aqueous Solution Using Surface-Enhanced Raman Scattering and Optical Fibers. Anal. Chem. 83, 5888–5894 (2011).

    CAS  PubMed  Article  Google Scholar 

  • Maquelin, K. et al. Identification of medically relevant microorganisms by vibrational spectroscopy. J. Microbiol. Meth. 51, 255–271 (2002).

    CAS  Article  Google Scholar 

Download references

Acknowledgements

This study was sponsored by the main Direction Program of Knowledge Innovation (KZCXZ-EW-402) and the Hundred Talents Program of the Chinese Academy of Sciences and Ministry of Science and Technology (2011AA060907).

Author information

Authors and Affiliations

Authors

Contributions

R.R.W. and F.Z. designed and performed the experiments. L.C. assisted in Raman experiment, L.X.C. gave a hand with cyclic voltammetry, C.W. and L.X.C. guided the SDS-PAGE assay and C.L.C. helped in cultivating strains. R.R.W., G.P.S., H.Q.Y. and F.Z. analysed the results and wrote the manuscript.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Electronic supplementary material

Supplementary Information

Supporting Information

Rights and permissions

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

Reprints and Permissions

About this article

Cite this article

Wu, R., Cui, L., Chen, L. et al. Effects of Bio-Au Nanoparticles on Electrochemical Activity of Shewanella oneidensis Wild Type and ΔomcA/mtrC Mutant. Sci Rep 3, 3307 (2013). https://doi.org/10.1038/srep03307

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1038/srep03307

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing