Abstract
Background
Spinal muscular atrophy (SMA) is an autosomal recessive childhood-onset neuromuscular disease with a carrier frequency of ~1:50. Mitochondrial abnormalities are widespread in patients with SMA. Disease carriers for SMA (i.e., the parents of patients with SMA) are viewed as asymptomatic for SMA disease. As far as we are aware, mitochondria have not been previously examined in SMA carriers, yet as they are maternally inherited, mitochondrial function in SMA carriers has putative implications for disease pathogenesis.
Methods
Fibroblast cell lines derived from SMA carriers and controls were obtained from two different sources and cultured under standard conditions. The mitochondrial membrane potential, reactive oxygen species (ROS) production, citrate synthase activity, and bioenergetic analysis were examined as measures of mitochondrial function. The mitochondrial genome was also sequenced in a subset of the fibroblast cell lines to identify any mitochondrial DNA variants.
Results
Here, we show a depolarized mitochondrial membrane potential, increased levels of reactive oxygen species, and reduced citrate synthase activity in SMA carriers compared with controls. A likely pathogenic variant in the MT-CO3 gene (which encodes subunit III of cytochrome c oxidase) was also identified in a paternal carrier.
Conclusions
This study was conducted as a preliminary investigation of mitochondrial function in SMA carriers. Our findings suggest that disease carriers of SMA show differences in mitochondrial function, indicative of a subclinical mitochondrial phenotype. Further investigation in a larger sample set is warranted.
Plain language summary
Spinal muscular atrophy (SMA) is a disease that mostly affects children in which the muscles become weaker over time, and often leads to death in untreated individuals. It is caused by a defective gene that children often inherit from their parents. The parents of children with SMA are known as disease carriers if they do not show any symptoms of SMA themselves. We studied skin cells from the parents of people with SMA and found changes in a component of the cells called the mitochondria. These changes are not normally present in healthy individuals. Further work is needed to fully understand the implications of our findings for those with SMA and their parents.
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Introduction
Spinal muscular atrophy (SMA) is a predominantly childhood-onset neuromuscular disease caused by loss-of-function mutations in the survival of motor neuron 1 (SMN1) gene1. SMN1 mutations show an autosomal recessive inheritance pattern, with a carrier frequency of around 1:502. SMN protein in humans is obtained from two highly similar genes, SMN1 and SMN2. Patients with SMA show reduced levels of SMN protein due to insufficient compensation of protein expression by SMN2. Without treatment and in its most severe forms, SMA leads to muscle atrophy, weakness, paralysis, and premature death. Current therapies restore SMN protein levels and have led to improved clinical outcomes for many patients3. However, not all patients respond to treatment and there is a need for combinatorial therapies.
To explore the possibility that inherited mitochondria may exhibit a subclinical phenotype, we examined mitochondrial function in fibroblast cells from SMA carriers, specifically, parents of patients with SMA. Mitochondrial abnormalities are found in patients and experimental models of SMA4. Moreover, in a mouse model of SMA, changes in mitochondrial morphology at an early postnatal stage5 suggest that mitochondrial function may be affected in utero. Mitochondria are maternally inherited, with this inherited population dividing to ultimately populate all cell types of the body6. Thus, the fitness of this initial population of mitochondria is critical for optimal health during development and beyond. Disease carriers of SMA have altered levels of SMN protein but are asymptomatic. Here, we show changes in the mitochondrial membrane potential, ROS levels, and citrate synthase activity in SMA carriers compared with controls.
Methods
Cell culture
Fibroblast cell lines were obtained from the HGRI Sample Repository for Human Genetic Research at the Coriell Institute for Medical Research (Camden, NJ, USA – cell lines GM03651; GM01650; GM03813; GM03814; GM03815 deposited with informed consent), or a collection maintained by Brunhilde Wirth (University of Cologne, Cologne, Germany) that were deposited with informed consent and approval of the ethics committee of the University Hospital of Cologne (approval numbers 04–138 and 13–022). They were not tested for mycoplasma contamination. All cell lines were authenticated regarding SMN1/2 genotype (Supplementary Data). The sex of each individual from whom each line was derived from was known (Supplementary Data). Fibroblasts were cultured in Dulbecco’s modified Eagle’s medium supplemented with 15% foetal bovine serum, 1% non-essential amino acids, and penicillin/streptomycin (all Gibco Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C in a humidified chamber of 95% air and 5% CO2.
Mitochondrial membrane potential
Fibroblasts were plated (0.5 × 104 cells/well) in triplicate into 96-well tissue culture plates and incubated overnight. Cells were incubated in a non-quenching concentration7 (25 nM) of tetramethylrhodamine, ethyl ester (TMRE) for 30 min at 37 °C, in accordance with the manufacturer’s guidelines (Abcam, Cambridge, UK). Relative fluorescence units (RFU) were corrected for mitochondrial content by the amount of TOMM20 protein (Invitrogen, Waltham, MA, USA; PA5-52843; 1:1,000 dilution), obtained independently by western blotting.
Mitochondrial ROS production
Fibroblasts were plated (3.0 × 104 cells/well) in triplicate into 96-well tissue culture plates and incubated overnight. Cells were treated with 5 μM MitoSOX Red for 30 min at 37 °C as described8 and in accordance with the manufacturer’s guidelines (Invitrogen). RFU were corrected for mitochondrial content by the amount of TOMM20 protein, obtained independently by western blotting.
Citrate synthase activity
Citrate synthase activity was measured in 1 × 106 cells in accordance with the manufacturer’s guidelines (Abcam). Citrate synthase activity was corrected by the amount of cellular protein, obtained independently by bicinchoninic acid assay (Thermo Fisher Scientific).
Bioenergetic analysis
Fibroblasts were plated at experimentally determined densities (3.0–5.0 × 104 cells/well; 3–5 replicates for each cell line) into Seahorse XF 24-Cell Culture Microplates (Agilent Technologies, Santa Clara, CA, USA) and incubated overnight. Bioenergetic analyses were performed using the Seahorse XFe24 Analyzer (Agilent Technologies), in accordance with the manufacturer’s guidelines. The Mito Stress Test protocol was performed with optimized doses of oligomycin (1 μM), FCCP (2 μM), and rotenone/antimycin (both 0.5 μM) to determine oxygen consumption rate (OCR) and extracellular acidification rate (ECAR). OCR and ECAR values were normalized to protein content, measured independently by sulforhodamine B assay9. Data were analyzed using Seahorse Analytics software.
Mitochondrial genome analysis
Genomic DNA was prepared from cell pellets of the SMA family trio in Cohort 1 (obtained from the Coriell Institute for Medical Research). DNA was referred to the NHS Highly Specialised Service for Rare Mitochondrial Disorders (Newcastle, UK) for mitochondrial DNA (mtDNA) analysis. Variants were identified by Next Generation Sequencing of mtDNA (Ion Torrent; Thermo Fisher Scientific) and alignment to the revised Cambridge reference sequence for human mtDNA (NC_12920.1) (Supplementary Data).
SMN genotyping
Genomic DNA was prepared from cell pellets using the QuickGene DNA Tissue extraction kit (FUJIFILM Wako Chemicals Europe GmbH, Neuss, Germany). Multiplex ligation-dependent probe amplification was used to identify SMN1 and SMN2 copy number (SALSA MLPA Probemix P021 SMA; MRC Holland, Amsterdam, the Netherlands).
Statistics and reproducibility
Statistical analyses were performed using GraphPad Prism (version 9.0.0 for Windows; GraphPad Software, San Diego, CA, USA). Normality of the data were determined using the Shapiro–Wilk test. Based upon normality, the data were then analyzed by two-tailed Student’s t-test or Mann–Whitney test. ‘Independent’ means that each experiment was performed on distinct weeks and from different cell passages and/or stocks. Experimental ‘n’ was used was used for statistical analysis10. Technical replicates for each experiment were averaged to generate an experimental mean, which was separately analyzed. Each experiment was performed at least three-times. P < 0.05 was considered statistically significant.
Reporting summary
Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.
Results
We used two independent cohorts of fibroblast cell lines to examine mitochondrial function in SMA carriers. All controls were homozygous for the functional allele of SMN1, while the carriers were heterozygous. The groups were balanced by age but not sex.
The mitochondrial membrane potential indicates the polarized state and functional status of mitochondria. We examined the membrane potential using TMRE, a dye that sequesters in mitochondria based upon their membrane potential. Compared with controls, SMA carriers showed a reduced (or more depolarized) mitochondrial membrane potential (Fig. 1a, b). Depolarization of the mitochondrial membrane potential can be caused by altered production of mitochondrial ROS. Therefore, we examined levels of mitochondrial ROS using MitoSOX, a dye that binds to superoxide species in mitochondria. We found an overall increase in ROS production in SMA carriers compared with controls (Fig. 1c, d).
As additional measures of mitochondrial function, we examined bioenergetic activity of the fibroblasts. Citrate synthase is the first enzyme in the tricarboxylic acid cycle and the final pathway for oxidation of nutrients. We observed reduced citrate synthase activity in SMA carrier fibroblasts compared with controls (Fig. 1e, f). However, further analysis of respiratory bioenergetics found no significant changes in measures of OCR (reflecting oxidative phosphorylation) or ECAR (medium acidification broadly due to metabolic processes) (Fig. 2a–c). Taken together, these have revealed modest changes in mitochondrial function in SMA carriers compared with controls.
Finally, to determine whether mtDNA variants are associated with SMA carriers and/or patients, the mitochondrial genome was sequenced in carrier (maternal, GM03814 and paternal, GM03815) and patient (GM03813) cell lines from Cohort 1. This identified a likely pathogenic variant (m.9931 G > A p.(Trp242*])) at ~10% heteroplasmy in the MT-CO3 gene of the paternal cell line (Fig. 2d). The MT-CO3 gene encodes subunit III of cytochrome c oxidase (i.e., Complex IV), but as the variant is only present in 10% of mtDNA molecules, its functional impact is unclear.
Discussion
The aim of this study was to determine whether fibroblast cell lines from asymptomatic disease carriers of SMA show altered mitochondrial function. Accordingly, we identified changes in the mitochondrial membrane potential, ROS levels, and citrate synthase activity in carriers compared with controls. The phenotype identified may reflect altered baseline mitochondrial activity in the fibroblasts. A depolarized mitochondrial membrane potential and increased ROS would typically be associated with a less healthy population of mitochondria. However, as mitochondrial stress can be viewed as means to build cellular resilience11, at least in certain cell types12, a more stressed population of mitochondria may not necessarily be harmful. Speculatively, depolarized mitochondria should be targeted for degradation through ubiquitin pathways13. SMN is proposed to play a role in ubiquitination14, therefore it could be interesting to investigate this process further in carriers. Alternatively, the phenotype may reflect a response to the artificial environment imposed by cell culture, not least the use of antibiotics to examine the function of an organelle of bacterial ancestry15. In this case, the phenotype may suggest that carriers have a less robust response to cellular stress.
The mtDNA variant identified in a paternal carrier has only been reported once previously in a case of mitochondrial myopathy with recurrent myoglobinuria16. It was detected at considerably lower heteroplasmy (10%) than the 50–60% heteroplasmy associated with primary mitochondrial diseases17. Being found in a paternal carrier, it will not be transmitted to the patient. Moreover, the absence of mutations in the maternal carrier and/or patient lines does not exclude their presence as mtDNA can be lost during culture of primary fibroblasts18. No marked differences in respiratory phenotype described by OCR/ECAR parameters were identified under the conditions examined. One consideration here is the use of a high glucose medium for cell culture: a bioenergetic mitochondrial phenotype may not be observed unless cultured cells are grown in a medium that forces them to rely on oxidative phosphorylation19.
This study was conducted as a preliminary investigation of mitochondrial function in SMA carriers. Altogether, it can be concluded that in this sample set of fibroblasts, SMA carriers show differences in mitochondrial function. However, a limitation of our study is the small sample size, impacted by limited availability of cell lines from carriers as well as matched controls. Future experiments would benefit from a more homogenous sample set (in terms of e.g., age, SMN2 copy number, SMA clinical subtype). Indeed, inspection of the data shows an apparent influence of age in certain assays (e.g., the TMRE assay; Fig. 1a), with age having a well-described influence on mitochondrial function (see e.g20.). Although direct extrapolation of these findings to other cell types and/or the in vivo situation is not possible, given the increasingly recognized role of mitochondria in cell fate processes21, including those related to neurodevelopment22, further understanding of the possible inheritance of altered mitochondrial function in patients may benefit families with SMA and possibly even other diseases with mitochondrial dysfunction as a pathological hallmark. Moreover, the identification of cellular phenotypes in SMA carriers suggests that more extensive investigations of subclinical phenotypes are now warranted as it could help better decipher the complexity of the disease, and ultimately lead to the discovery of combinatorial therapy targets.
Data availability
The data supporting Figs.1 and 2 are provided in the Supplementary Data. All raw data are available from the corresponding author upon request.
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Acknowledgements
We thank Dr Charlotte Alston (NHS Highly Specialised Service for Rare Mitochondrial Disorders, Wellcome Centre for Mitochondrial Research, Newcastle Upon Tyne) for her help with the mitochondrial genome analysis. This work was completed as part of a Daphne Jackson Trust Fellowship awarded to RJ and funded by Medical Research Scotland. This work was also supported by the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation program under the Marie Sklodowska–Curie grant agreement No. 956185 (SMABEYOND) (to EJNG, BW, THG). KMEF is supported by an Academy of Medical Science Starter Grant for Clinical Lecturers and an MRC Clinician Scientist Fellowship (MR/X018288/1).
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Conception and design of the study: R.J., T.H.G.; Acquisition and analysis of data: R.J., K.M.E.F., E.J.N.G., B.W.; Drafting the manuscript and figures: R.J., K.M.E.F., T.H.G.
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The authors declare the following competing interests: T.H.G. has provided advisory services to Roche and Novartis. The other authors declare no competing interests.
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James, R., Faller, K.M.E., Groen, E.J.N. et al. Altered mitochondrial function in fibroblast cell lines derived from disease carriers of spinal muscular atrophy. Commun Med 4, 86 (2024). https://doi.org/10.1038/s43856-024-00515-w
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DOI: https://doi.org/10.1038/s43856-024-00515-w