## Introduction

Arctic sea ice, characterized by strong seasonal variations, is a critical component in the global climate system as sea ice has direct impact on climate change by regulating energy exchanges between the atmosphere and ocean. The variability of sea ice contributes to changes in the albedo effect, the freshwater system, and the surface energy budget in the Arctic Ocean1,2,3,4. In a positive ice-albedo feedback, increases of absorbed energy result in further sea-ice melting5,6. Increased melting and sea-ice export can slow down the Atlantic Meridional Overturning Circulation (AMOC) as well as the poleward heat transport7. Due to these complex feedback processes, the Arctic is both a contributor to climate change and a region that is most strongly affected by global warming3,4,8,9.

Arctic sea ice has reduced drastically over the past few decades, recognized in both satellite observations and model simulations8,10,11. Since 1980, the sea-ice cover has had a mean annual areal reduction of ~20% and an even stronger decrease of ~30% in September7. This decrease in sea ice is also suggested to be the driver of frequent cold extremes over Eurasia in the past two decades12. Historical observations spanning the last few decades are deficient in length to decipher the processes of accelerated sea-ice retreat, thus longer-term and high-resolution proxy records of paleoenvironmental changes are needed. During transition from the last deglacial to the Holocene, the climate system underwent numerous abrupt changes, particularly the Bølling/Allerød (B/A) interstadial and the Younger Dryas (YD) stadial. The Holocene, not concluded yet, has experienced a significantly warmer period during its early stage (Early Holocene Thermal Maximum)13. Causes of the abrupt climate change are not fully understood, hence special emphasis on the transition from the deglacial into the Holocene is of significance to understand the forcing mechanisms of the climate system. In this context, the Arctic marginal seas characterized by strong seasonal variability in sea-ice cover, primary productivity, and terrigenous (riverine) input are very sensitive to environmental changes and thus of major importance for paleoclimate reconstructions. One of these marginal seas is the Beaufort Sea (Fig. 1), which we focus on in this paper.

Today, the Mackenzie River is the largest fluvial source of water and sediment into the Beaufort Sea, characterized by water discharge of 316 km3 yr−1 and sediment flux of 124–128 Mt yr−1 (Ref. 14; Figs. 1 and 2). Since the drainage system was established during the late Wisconsinan (i.e., Marine Isotope Stage 2) as a result of glacial erosion, it was an outlet of glacial meltwater during the last deglaciation15. During this period, a cold episode known as YD coincides with a significant reduction of AMOC caused by increased freshwater flux into the North Atlantic deep-water formation region16. The freshwater discharge has been linked to an outburst from the Lake Agassiz, and there is an ongoing debate about the pathway of this freshwater discharge, i.e., whether it drained first into the Arctic Ocean and then to the Atlantic Ocean or whether it drained directly into the Atlantic Ocean17,18,19,20 (Fig. 3). More recently, field data indicate the freshwater of Lake Agassiz could not drain northward during the YD21, while model simulations suggest the meltwater drainage via Mackenzie River (northwestern outlet) as a competitive candidate triggering the YD cooling17,22. Following the YD cold period, field data from the Fort McMurray and the Mackenzie Delta point to a post-YD flood from proglacial lakes, which drained through the Mackenzie River to the Arctic Ocean during the early Holocene23,24. The timing of the flood coincides with the Preboreal Oscillation (PBO) and thus it has been proposed as the trigger of PBO25. Above-mentioned events are of significance to climate change, but corresponding marine records are scarce. Therefore, high-resolution records are needed to identify these events and the timing of their inception.

Here, we apply a biomarker approach on a well-dated sediment core from the Beaufort Sea directly off the Mackenzie River (Fig. 1), covering the time interval of the last deglaciation and the Holocene. Multiple biomarker proxies are used to reconstruct the changes in sea ice, sea surface temperature (SST), primary productivity, and terrigenous input. Based on our records, we demonstrate that (1) the Beaufort Sea region was nearly ice free with variable SSTs during the last deglaciation and the early Holocene, (2) sea-ice cover developed during the mid-late Holocene, coinciding with a drop in terrigenous sediment flux, SST, and primary production, and (3) two major events, characterized by prominent maxima in sediment flux, occurred at 12.83 ± 0.15 and ca. 11 kyr bp. Whereas the former is related to the YD flood event, the origin of the second event is related to a post-YD flood and/or coastal erosion.

## Results

### Chronology and approach

Core ARA04C/37 was recovered during the 2013 Araon Cruise ARA04C26 at the Beaufort Sea continental slope off the Mackenzie River (70°38.0212′N, 139°22.0749′W; 1173 m) (Fig. 1), an area characterized by high sedimentation rates. The highest sedimentation rates occur at the Mackenzie Trough (~40–320 cm kyr−1) as well as the continental shelf and slope nearby (~100–200 cm kyr−1) (Ref. 27 and references therein).

The chronology of Core ARA04C/37 was constrained by accelerator mass spectrometry (AMS) 14C dating on calcareous foraminifera (planktic and mixed species; Fig. 4a and Supplementary Table 1, see “Methods”) and, in the uppermost centimeters, excess 210Pb. The age–depth model is established by a combination of AMS14C dates of cores ARA04C/37 and JPC15 (Fig. 4)19, based on the good correlation of AMS14C dates and magnetic susceptibility (Figs. 4a and 5a, b) from both cores (see “Methods”). Ages in this paper are provided as calendar years bp. The age–depth relation reveals a mean sedimentation rate of 27 and 100 cm kyr−1 in the upper and lower 300 cm, respectively, with high sedimentation rates in two layers near 13 kyr bp (450–500 centimeters below seafloor (cmbsf)) and 11 kyr bp (295–375 cmbsf). The core represents the deglacial to Holocene transition in high resolution, including the B/A and YD intervals. This enables reconstructions of sea ice, primary productivity, SST, and freshwater discharge in the Beaufort Sea, with special emphasis on the flood events during the transition, in great detail.

The sea-ice biomarker IP2528,29 in combination with open-water phytoplankton biomarkers (e.g., dinosterol), the so-called “PIP25” index, allows semi-quantitative reconstructions of sea-ice concentrations (e.g., Refs. 30,31). The newly developed ring index (RI-OH′) of hydroxylated isoprenoid glycerol dialkyl glycerol tetraethers (OH-GDGTs) has been used as a potential tool to reconstruct SST in cold environments (ca. <15 °C)32. The biomarkers brassicasterol and dinosterol are indicators for marine phytoplankton, whereas ß-sitosterol and campesterol are indicators for terrigenous input33,34,35. The brassicasterol in some settings has significant sources from freshwater33,36, which is the case in our study (discussed below), and hence we focus on dinosterol to reconstruct marine primary production (Supplementary Fig. 1a, b). The branched GDGTs (b-GDGT) are indicative for terrestrial input37,38,39,40, and the FC32 1,15 index (fractional abundance of C32 1,15-diol) is indicative for aquatic riverine input, as the C32 1,15-diol is found particularly abundant in freshwater systems41,42,43,44. More details can be found in the method section.

### Deglacial–Holocene changes in sea ice and primary production

During the deglaciation and the early Holocene (~14–8 kyr bp), minimum concentrations of IP25 (close to the detection limit) and low PIP25 values point to dominantly ice-free conditions (Figs. 6a and 7c and Supplementary Fig. 1c). In such a situation, open-water conditions would be expected to promote primary production. However, concentrations of dinosterol (~1–5 µg g TOC−1) were low to moderate, implying low primary production during the B/A interstadial (Fig. 6c). This may be explained by the fact that the Beaufort Sea itself is an oligotrophic system6, and the Bering Strait was still closed at that time (>13 kyr bp), hence preventing the inflow of nutrient-rich Pacific Water45,46. Primary productivity shortly increased during the early YD (Figs. 6c and 7e), probably promoted by higher nutrient levels supplied by the YD flood (see below). In the early Holocene, the primary productivity experienced a rapid increase at ca. 11 kyr bp as well as subsequent moderate increases during the early Holocene (Fig. 7e), possibly caused by high terrigenous input (including nutrients) and the total inundation of Bering Strait, respectively45. Furthermore, maximum summer insolation47 may have caused shorter sea-ice seasons at the core site. More open waters and longer duration of ice-free conditions were favorable for dinosterol production in spring and summer.

During the mid-late Holocene (8–0 kyr bp), sea ice expanded, as evidenced by elevated IP25 and PIP25 values (Figs. 6a and 7c), which reveals an evolution from dominantly ice-free conditions (PIP25 < 0.2) to more extended ice-cover conditions (PIP25 > 0.8) due to decreasing summer insolation. In turn, expanded sea-ice cover and longer sea-ice duration reduced primary production since the middle Holocene (ca. 8–2.5 kyr bp) as clearly reflected in decreased dinosterol accumulation rates (Fig. 7e). The total organic carbon (TOC)-normalized dinosterol concentrations differ from dinosterol accumulation rates during the middle Holocene, increasing between ca. 8–5 kyr bp (Fig. 6c). This is attributed to simultaneous decreases in marine primary production and terrestrial input, due to sea-ice formation and decrease in river discharge, respectively. During this phase, the reduction of terrestrial organic carbon input was probably larger than that of marine organic carbon input, resulting in increased TOC-normalized dinosterol concentrations. During the late Holocene (ca. 2.5–0 kyr bp), an extended sea-ice cover even hindered primary production by limiting light penetration and nutrient release from sea-ice melt (Fig. 7c, e).

### Reconstruction of marginal ice zones (MIZs)

Abundant phytoplankton production is commonly found in MIZs. In these regions, melting sea ice releases nutrients and less-saline waters into the surface layer, forming a stable and nutrient-rich environment above the pycnocline, i.e., favorable conditions for algae blooms48,49,50,51.

Dinosterol and HBI-III (Z-isomer) (further referred to as “HBI-III”; see “Methods”) have both been proposed to indicate pelagic algal production. Studies of surface sediments from different polar regions show significant enhancement of HBI-III in the MIZs52,53,54,55, while no significant difference in abundances or distributions of phytoplankton sterols was found between the MIZs and open waters53,54. Thus, the HBI-III has been proposed as an MIZ indicator by these authors. Cross-plots of IP25 vs. HBI-III show a better correlation than IP25 vs. dinosterol (Supplementary Fig. 1c, d), supporting that HBI-III is closely associated with sea-ice environments. Hence, HBI-III is more or less absent when sea-ice cover is strongly reduced or absent (i.e., between ~9 and 14 kyr bp; Fig. 7b, c, e). The IP25 and HBI-III accumulation rates peaked at ca. 5.6 and 3.5 kyr bp (Fig. 7d, e), probably representing two periods of MIZ situations (see details in “Discussion”).

### Deglacial–Holocene changes in freshwater discharge

Synchronous changes (Fig. 6d, e) as well as the positive correlation (Supplementary Fig. 1a) between terrigenous biomarkers (campesterol and ß-sitosterol) and brassicasterol suggest that brassicasterol in our record has a mixed source from freshwater and marine algae.

During the last deglaciation and the early Holocene (~14–8 kyr bp), very negative δ13Corg values of −28 to −27‰ (Fig. 6f), relatively high terrigenous biomarker accumulation rates of 5–20 µg cm−2 kyr−1 (Fig. 7f), and high FC32 1,15 values up to 0.5 (Fig. 7g) all point to high terrigenous input. Relatively warm and wet environments promoted terrigenous matter transport, and low sea-level conditions resulted in a closer distance of the core site to the river mouth receiving more terrigenous matter. In the mid-late Holocene (8–0 kyr bp), δ13Corg values increased (>−27‰) and terrigenous biomarker accumulation rates decreased to 1 µg cm−2 kyr−1 (Figs. 6f and 7f). Similarly, FC32 1,15 values decreased to 0.3 (Fig. 7g), reflecting a descending river discharge and an increasing distance of the core site to the river mouth (cf. Ref. 56).

### Sea surface temperature variations

During the deglaciation (~14−11.7 kyr bp), SST values were variable and higher in comparison to the mid-late Holocene SSTs (Fig. 7a), indicating relatively warm and open-water conditions. Open-water conditions with less sea ice allowed sufficient heat exchange between ocean and atmosphere. Hence the SST variations followed the global climate history recorded in the Greenland ice core57 (Fig. 7a, j), with typical higher RI-OH′-SST values in the B/A interstadial and lower SST values during the YD stadial (Fig. 7a). In the early Holocene (11.7–8 kyr bp), RI-OH′-SST values rose again (Fig. 7a), most likely attributed to the summer insolation maximum. Additionally, the total inundation of the Bering Strait occurred at ca. 11 kyr bp and the Pacific Water inflow may have contributed warm water to this site45. In the mid-late Holocene (8-0 kyr bp), RI-OH′-SST values were quite low and stable (Fig. 7a), reflecting expanded/seasonal sea-ice conditions in the mid-late Holocene (Fig. 7c).

### Major sediment flux events at 13 and 11 kyr bp

Two distinct peaks at ca. 13 and 11 kyr bp are found in the accumulation rates of terrigenous biomarkers, dinosterol, and bulk sediment (Fig. 7e, f and Supplementary Fig. 2). The first peak may reflect the YD flood event (cf. Ref. 19) characterized by large amounts of terrigenous material and nutrients into the Beaufort Sea. Accumulation rates of terrigenous biomarkers ß-sitosterol and campesterol as well as b-GDGTs started to increase at 12.83 ± 0.15 kyr bp (Fig. 7f, h), close to the onset of Beaufort Sea freshening (12.94 ± 0.15 kyr bp) estimated by Keigwin et al.19,58. The YD flood transported large amounts of heterogeneous sediments into the Beaufort Sea and resulted in increases in magnetic susceptibility, density, and (probably detrital59,60) CaCO3 contents (Fig. 5b–d). These sediments were most probably non-biogenic/organic and thus have caused a significant decrease of TOC content (Figs. 5e and 6g and Supplementary Fig. 2). Such changes in physical and chemical properties occurred slightly before the YD flood, probably attributed to culminated meltwater production in the northwestern outlet area at the end of B/A.

In the earliest Holocene, an even stronger peak in terrigenous input occurred (Fig. 7f) at ca. 11 kyr bp. High heterogeneous sediment flux resulted in similar characteristics as caused by the YD event (Fig. 5b–e). Although the second event is characterized by high sediment flux and coincides with the post-YD flood, its origin could not be unambiguously proven yet. The second event may have partially different sediment sources from the YD event (see “Discussion”).

## Discussion

Core site ARA04C/37 is particularly sensitive to changes in paleo sea-ice cover, because it is located in the area of modern seasonal sea-ice cover (Fig. 2). In contrast to a foraminifera-based study on the nearby Core 750 that proposed a permanent ice cover during the last deglaciation (14–11.5 kyr bp)61, the records of PIP25 and RI-OH′-SST in Core ARA04C/37 clearly point to open-water conditions that allowed heat exchange with the atmosphere (Fig. 7a, c). Additionally, the minor differences of 14C ages between planktic and benthic foraminifera (Supplementary Table 2) indicate that the surface and bottom water masses were relatively well ventilated, further corroborating the scenario of ice-free conditions during the last deglaciation. During the early Holocene, on the other hand, both the foraminifera study61 and our biomarker records (Fig. 7c) display more open-water conditions in comparison to the modern Beaufort Sea.

The Beaufort Sea itself is an oligotrophic system6 and the nutrient-rich Pacific Water inflow is an important nutrient source stimulating primary productivity. Studies (foraminifera, geochemical, and geophysical data) from sediment cores suggest an initial opening of Bering Strait at about 11.5 kyr bp46,62,63, whereas other evidence based on marine species dispersal suggests an earlier connection at about 13.3 kyr bp64,65,66. These contradictory evidences are recently reconciled by a gravitationally self-consistent sea-level simulation45. The authors predict the first opening of Bering Strait with shallow inundation at ca. 13 kyr bp. Substantial melting of the Cordilleran Ice Sheet and western Laurentide Ice Sheet (LIS) may have resulted in a local sea-level stillstand during 13–11.5 kyr bp, and then the total inundation of the Bering Strait occurred until ca. 11.5 kyr bp45. Primary production in our records remained relatively low during the deglacial to Holocene transition and generally increased from the early Holocene, except for two rapid increases triggered by high terrigenous input (Fig. 7e, f). It implies that even if the Bering Strait opened at 13 kyr bp, probably because of its shallow inundation, the Pacific Water influence on primary production in the southern Beaufort Sea was small. Upon the total inundation of the Bering Strait at the early Holocene, primary production started to increase, reflecting more influence of Pacific Water inflow.

HBI-III has been proposed as an indicator for MIZ52,53,54,55. Among these studies, a study of surface sediments from the Barents Sea found that HBI-III maxima are correlated with the winter ice edge and thus proposed HBI-III as an indicator for winter MIZ53. This is challenged by a study from the East Greenland shelf where an enhancement of HBI-III was observed near the mid-July ice edge in an East Greenland fjord55. The difference in seasonality is controlled by the general sea-ice situations in both areas. Major parts of the Barents Sea are more or less ice free during late spring, summer, and autumn, and thus the ice edge is restricted to the cold/winter season. Along the East Greenland continental margin controlled by the East Greenland Current, on the other hand, sea ice is much more extended throughout the year and thus, the ice edge is more restricted to the summer season.

In our case, HBI-III production was enhanced twice during the Holocene (Fig. 7e). In the early Holocene, i.e., prior to the first HBI-III peak, it was nearly ice free throughout the year, reflected in very low PIP25 values < 0.2 and variable SSTs (Fig. 7a, c). Then, a general trend toward an increasing sea-ice cover is coinciding with weakened insolation (Fig. 7c, j). Due to sea-ice expansion, the core site was first proximal to an MIZ situation during cold seasons, when HBI-III peaked at ca. 5.6 kyr bp (Fig. 7e). The interval between the two HBI-III peaks (ca. 5–4 kyr bp) characterized by a clear decrease in HBI-III probably represents seasonal sea-ice conditions. As sea ice further expanded, the second period of MIZ may have existed during warm seasons at ca. 3.5 kyr bp, shown by peaked HBI-III (Fig. 7e). During the last 2.5 kyrs, reduced HBI-III and dinosterol production (Figs. 6b, c and 7e) implies a more extended ice cover. Sea-ice variability is illustrated in Fig. 8.

A novel proxy HBI TR25 shows strong associations with the satellite-derived spring chlorophyll a concentration in the Barents Sea, and thus has been proposed as a spring phytoplankton bloom proxy67. Although the proxy is restricted to a regional area, the HBI TR25 determined in Core ARA04C/37 seems to support our reconstruction of the MIZ history and by this the applicability of the proxy. Higher values of HBI TR25 may point to enhanced occurrence of spring blooms between 8 and 5 kyr bp, indicating that the core site was relatively ice free in spring. Hence, a winter MIZ may have existed at certain times within this period. On the other hand, lower values of HBI TR25 may suggest reduced numbers of spring blooms from 5.6 kyr bp to present. One of the explanations is the existence of a spring sea-ice cover, which may have limited the spring blooms. This coincides with the expanding sea-ice cover and longer sea-ice duration during this time interval. To some extent, a summer MIZ may have existed occasionally within this period. These scenarios are in agreement with the HBI-III record, suggesting that the core site was probably close to a winter MIZ at ca. 5.6 kyr bp and to a summer MIZ at ca. 3.5 kyr bp.

The changes in RI-OH′-SST values seem to correlate with changes in sea-ice concentrations. That means quite high but variable temperatures occurred during dominantly ice-free periods, while low but stable temperatures during ice-covered periods (Fig. 7a, c), suggesting the proxy’s potential as a tool for temperature reconstruction in the Arctic Ocean. To our knowledge, the RI-OH′-SST proxy was first studied on surface sediments from the Chinese coastal seas and the Yangtze Estuary, and then a global surface sediment compilation was assessed32. Samples from northern high latitudes are mainly collected from the Fram Strait68. In these samples, residual SSTs derived from RI-OH′-SST minus remote sensing SST are relatively small but generally above zero. Therefore, the absolute values should still be interpreted with caution. Nevertheless, based on the close association between the RI-OH′-SSTs and the sea-ice concentrations, we propose that the proxy most likely reflects winter/spring SSTs in the southern Beaufort Sea. Low and stable SSTs during the seasonal sea-ice periods (mid-late Holocene) (Fig. 7a, c) suggest that RI-OH′ may record SST in the ice-covered seasons, likely in winter. As insolation declined in the mid-late Holocene, ice-covered seasons might have prolonged and persisted into early spring.

Superimposed on the long-term changes in sea ice, primary production, terrestrial input, MIZ and SST are two extremely high sediment flux events during the deglacial to Holocene transition (at ca. 13 and 11 kyr bp), which are related to the YD flood and the post-YD flood events.

The YD cooling event (12.9–11.7 kyr bp) was the longest interruption to the gradual climate warming since the severe Last Glacial Maximum (Fig. 7j). Meltwater of LIS draining through the Arctic Ocean to the Nordic seas has been suggested to have profound impacts on slowing down the AMOC and triggering the YD cooling17,22. Glacial system modeling, and seismic reflection data as well as physical properties from the Beaufort slope, shows massive floods possibly via a northwestern outlet into the Arctic Ocean at the onset of the YD (Fig. 3)22,69. Recently, Keigwin et al.19 provided initial freshening evidence of the southern Beaufort Sea. Core JPC15 recorded the freshening in a decrease of δ18O values of the planktic foraminifera Neogloboquadrina pachyderma by at least 1‰ at the start of the YD (Fig. 7i). In a pilot work, paleosalinity reconstruction from δ2H of palmitic acid in the same region (Core PS72/291-2; Fig. 1) shows a significant reduction of salinity directly prior to the YD70. Other evidence, e.g., decreased δ18O values of planktic foraminifera found in the Laptev Sea, Yermak Plateau, Amundsen Basin, and Mendeleev Ridge71,72,73,74, a prominent increase in sea-ice cover in the Laptev Sea continental margin35, and a pulse in detrital dolomitic limestones (Arctic-Canadian sediment source) in the central Arctic Ocean59, point to a YD-age Arctic freshening sourced from the LIS (Fig. 3)75.

Meltwater production from the northwestern sector of the LIS might be a component causing the Beaufort Sea freshening. Model simulation suggests that runoff to the Arctic Ocean from melting Keewatin Dome alone was sufficient to slow down the AMOC during YD22. Thus, it is possible that such melting culminated at the end of the B/A and strengthened freshening. This is supported by changes in physical properties of Core ARA04C/37 that slightly predated YD (Fig. 5). Another possible freshwater component is the outburst from proglacial/subglacial lakes, such as Lake Agassiz. Our multiple biomarker records also document this YD event and suggest the freshening was more related to a flood event as outlined in the following (Figs. 5 and 7).

B-GDGTs are synthesized by bacteria and can be found in numerous terrestrial settings, e.g., soils, lakes, and rivers37,38,39,40. As freshly deglaciated surfaces probably have negligible soil organic matter, the increases of b-GDGTs at the onset of the YD does likely not indicate input from soils. On the other hand, increase of meltwater in the rivers would not increase b-GDGTs production. Hence the main contribution of b-GDGTs increases seems not from the rivers. Another possible source could be from the melting ice sheet, i.e., b-GDGTs might have been entrained into the base of the ice sheet when it glaciated and were released when it deglaciated. However, such release and accumulation of b-GDGTs should be continuous processes. The sharp increase and peak accumulation of b-GDGTs at the start of the YD (Fig. 7h) point to lakes as the most probable source. In such lakes b-GDGTs could have been accumulated in a relatively stable environment for a long time and then been delivered to the Beaufort Sea within a short period.

This interpretation is supported by the riverine input proxy FC32 1,15 (fractional abundance of C32 1,15-diol). The C32 1,15-diol is found particularly abundant in freshwater systems41, and its production has been related to flow regimes76. A study from the Amazon River shows that the highest abundance of C32 1,15-diol exists in water bodies with low flow velocity and low turbidity, while the lowest abundance of C32 1,15-diol is found in conditions with high turbidity and high flow velocity76. This suggests that a high-velocity flow regime like a flood producing higher turbidity would limit the C32 1,15-diol production. Thus, the significant drop in FC32 1,15 values during the YD (Fig. 7g) characterizes a flood event of high erosive power and high flow velocity in turbid waters.

Assuming that the high-resolution terrestrial records of Core ARA04C/37 represent the YD flood event, a duration was estimated based on records of terrigenous sterols and b-GDGTs (Figs. 6d and 7h). The peak discharge started at 12.78 ± 0.15 kyr bp and ended up at 12.63 ± 0.13 kyr bp, resulting in a flood duration of about 150 years. Such estimate is close to the duration of 130 years estimated by Keigwin et al.19.

Although our terrestrial biomarker records in combination with previous paleosalinity reconstructions19,70 indicate a strong YD flood, the flood water source still remains unclear. Coinciding with the YD event, a large lake-level drop of Lake Agassiz (with an estimated freshwater release of ~17,000 km3; Ref. 77) has been proposed as the freshwater source23. According to more recent studies, however, no clear field evidence (ice margins or shorelines) supports that Lake Agassiz drained to any northwestern outlet before 10.8–10.1 kyr bp21,24. It cannot be excluded that the northwestern outlet of Lake Agassiz opened at an earlier phase and its evidence has been removed by a readvance of LIS. Otherwise the flood may have other water sources, e.g., outburst from further northern proglacial/subglacial lakes. If so, the freshwater discharge from these lakes alone was too small to trigger the YD cooling. Besides freshening in the Beaufort Sea, input of freshwater during the YD was also found in the Northeast Pacific sea (Ref. 78 and references therein). A recent model simulation suggests that if the Bering Strait partially opened at the start of YD45, some of the freshwater from the Northeast Pacific could be transported through the Arctic Ocean to the Nordic seas, contributing to a collapse of the AMOC78.

In the earliest Holocene, following the YD flood, a post-YD flood at ca. 11.3 kyr bp has been identified by field data from the Fort McMurray area, and the freshwater source is suggested to derive from proglacial lakes McMurray, Meadow, and Churchill24. Evidence in the Fort McMurray region is in line with field data from the Mackenzie Delta supporting that a second high-energy fluvial episode occurred in the delta between 11.7 and 9.3 kyr bp23. More recently, seismic and geophysical data from the Beaufort margin also indicate that the second event probably initiated at ca. 11.3 kyr bp69. The timing of the second flood coincides with the PBO, thus it has been proposed that the second flood may have triggered the PBO25,69. As a large amount of freshwater from the Baltic Ice Lake entered the Arctic marginally predating the PBO79, Klotsko et al.69 regarded the second flood injection as a tipping point for sufficient weakening of the AMOC during the PBO.

Despite the field data indicate a second flood, direct evidence for freshwater input is still missing from the marine records. The δ18O record of Core JPC15 shows minimum values only during the YD flood, whereas values were even higher than the 2‰ baseline during the second event (Fig. 7i)19. A similar case was described for a piston core from the Mackenzie Trough, where a coarse-layered unit was dated to 11.5–11.3 kyr bp80, while the very positive δ18O values (~2.7–3.5‰) argued against the hypothesis of an outburst from proglacial lakes. So far, the only freshwater evidence is found in Core P189AR-P45 (see Fig. 1 for location), showing a drop of δ18O values at ca. 11.5 kyr bp81. However, since the age–depth model of this core is not well constrained81, the timing of the freshwater signal might be shifted to the YD period when improving the age model. In comparison to the YD flood, biomarker records also show slight differences during the second event (Fig. 7g, h). Moderate values of FC32 1,15 during the second event indicate relatively stable flow regimes and smaller discharge than the YD flood (Fig. 7g). Otherwise extremely high flow velocity would limit C32 1,15-diol production. Accumulation of b-GDGTs was also much smaller than during the YD (Fig. 7h), and seems to have different sources. This evidence gives rise to the question whether the high sediment flux was caused by a flood or whether it was controlled by other processes.

In this context, one should keep in mind that the second event also coincided with global meltwater pulse 1B (MWP 1B). Recent studies in the Okhotsk Sea and Pacific Beringia provided direct evidence of coastal erosion induced by the rapid sea-level rise82,83. In these records, two distinct maxima of terrigenous material were observed within the rapid sea-level rise at ca. 14 and 11 kyr bp (MWP 1A and 1B). In the Kara Sea, high terrigenous sediment fluxes characterized by significantly increased C/N ratios and refractory organic matter were recorded at about 11 kyr bp and also related to increased coastal erosion due to the postglacial flooding of the shelf sea84,85. In the Labrador Sea, a high sedimentation layer containing increased detrital carbonates was dated to ca. 11.5–11.3 kyr bp86. Although the authors related it to “Heinrich event 0,” almost simultaneous signals widely found in the Arctic may support the interpretation of sea-level rise induced coastal erosion. It is therefore possible that the high sediment flux during the second event was partially caused by coastal erosion and subsequent deposition of the eroded terrigenous material.

Although field data indicate a second flood during the early Holocene, marine evidence suggests that the high sediment flux could be caused by a meltwater flood and/or shelf flooding induced coastal erosion. Further work, e.g., comprehensive proxy reconstruction of freshwater discharge and paleosalinity (cf. Ref. 70) as well as carbon source constraint (cf. Ref. 82), is hence needed to distinguish different processes.

## Methods

### Chronology

Radiocarbon dates of Core ARA04C/37 and JPC15 are consistent during the last deglaciation (Fig. 4). A red vertical line is placed close to the onset of the magnetic susceptibility rise (Fig. 5a, b), and the almost same 14C ages from both cores at the vertical line suggest a good age constraint of the onset of magnetic susceptibility change. 14C dates from the two cores are highly consistent below 400 cmbsf (Fig. 4a). However, age offsets are enlarged from near 350 cmbsf toward the core top (Fig. 4a), indicating a weakened correlation between Core ARA04C/37 and JPC15. Therefore, we established the age–depth model for core ARA04C/37 by including six AMS14C dates from Core JPC15 below 400 cmbsf (Supplementary Table 1). Paired dating shows a mean difference of around 200 years between planktic and benthic foraminifera (Supplementary Table 2). Considerable ventilation allows mixed species (planktic and benthic) being used for age constraints. As discussed in detail by Keigwin et al.19, ΔR was defined as 200 ± 100 years in YD and 0 ± 100 in the Holocene and B/A in the age model. The age–depth model was developed using the “Bacon” software of Blaauw and Christen87. Bulk sediments of the top cm of the core were analyzed by gamma spectrometry (HPGe planar detector). Based on excess 210Pb in the uppermost centimeters and detectable anthropogenic 137Cs in the top sample, surface sediments were identified to be modern, therefore, the core top was fixed to 0 kyr bp. Between 450 and 500 cmbsf (unit 1), 14C dates are constant (mean 14C age of 11,160 years) and are indicative for event 1 (Fig. 4a). Although the onset of event 1 is well constrained, because there is no clear termination of event 1 shown in magnetic susceptibility, the range of unit 1 is less constrained. This might lead to an over- or underestimate of sedimentation rate. Similarly, despite that magnetic susceptibility in both cores shows generally synchronous changes between 375 and 420 cmbsf (Fig. 5a, b), enlarged age offsets indicate a weaker correlation between the two cores (Fig. 4a and Supplementary Table 1). Therefore, we define the unit 2 (event 2) mainly based on four constant 14C dates (mean 14C age of 9750 years) between 295 and 375 cmbsf, with poor constraints in both onset and termination of the event. The second high sedimentation unit (295–375 cmbsf) may be thicker than shown by the four dates, therefore we kept the four dates for more precise age model constraints, even though the last date of the plateau is not strictly increasing (Fig. 4). A “boundary” function was applied in the two units, which may have experienced distinctively high sedimentation rates (cf. Ref. 88). All the 14C ages were transformed into calendar ages following Reimer et al.89 via the Marine13 curve.

### Bulk parameters

For organic geochemical analyses, sediment samples were freeze-dried, grounded, and homogenized. TOC contents were determined by a Carbon–Sulfur Analyser (CS-125, Leco) after decarbonization with hydrochloric acids. Total carbon (TC) contents were analyzed by a Carbon–Nitrogen–Sulfur Analyser (Elementar III, Vario), and used for calculation of carbonate contents (CaCO3 = (TC − TOC) × 8.333). Carbon isotope composition of organic matter (δ13Corg) was analyzed by means of a Thermo Delta V Isotope Ratio Mass Spectrometer connected to a Thermo Flash 2000 CHNS/OH Elemental Analyzer employing continuous flow, performed at the Korea Polar Research Institute. δ13Corg values are given in per mil notation relative to Vienna Pee Dee Belemnite International Standard. Reference gases were calibrated relative to Indiana University Acetanilide#1, USGS40, Urea, and Thermo Soil standards. For a randomly selected set of samples, duplicate analyses were carried out to determine the analytical error. Analytical precision is within 0.2‰.

### Biomarker analyses

Freeze-dried sediments (5 g) were ultrasonically extracted with DCM:MeOH (2:1, v/v), and internal standards (0.076 μg 7-hexylnonadecane (7-HND) and 10.7 μg 5α-androstan-3β-ol (androstanol)) were added prior to analytical treatment. Total lipid extracts were concentrated and separated via open column chromatography with silica gel (6 mm i.d.*4.5 cm). The lipids were eluted by 5 ml n-hexane for hydrocarbon fraction, followed by 9 ml ethyl acetate:n-hexane (1:4 v/v) for sterol fraction (containing diols). The sterol fraction was further derivatized with 200 μl bis-trimethylsilyl-trifluoracet-amide (60 °C, 2 h). The composition of the hydrocarbons, sterols, and diols was analyzed by gas chromatography (GC)–mass spectrometry (MS) (Agilent 7890GC-Agilent 5977 A). Compounds were identified by comparison of GC retention times with published mass spectra (highly branched isoprenoids/HBIs: Refs. 28,90; sterols: Refs. 33,91; diols: Refs. 92,93).

For quantification of HBIs, their molecular ions (m/z 350 for IP25, m/z 348 for HBI II, and m/z 346 for HBI-III) were used in relation to the fragment ion m/z 266 (internal standard 7-HND). The sterols, brassicasterol (24-methylcholesta-5,22E-dien-3β-ol), campesterol (24-methylcholest-5-en-3β-ol), β-sitosterol (24-ethylcholest-5-en-3β-ol), and dinosterol (4α,23,24R-trimethyl-5α-cholest-22E-en-3β-ol) were quantified as trimethylsilyl ethers. Their molecular ions m/z 470, m/z 472, m/z 486, and m/z 500 were used in relation to the molecular ion m/z 348 of androstanol. External calibration curves (cf. Ref. 35) were applied and specific response factors were calculated for these ions. For quantification of the relative abundance of the diols, the fragment ions m/z 313.3 (C28 1,13-diol, C30 1,15-diol) and 341.3 (C30 1,13-diol, C32 1,15-diol) were used.

Reconstruction of sea-ice concentrations (PIP25) followed Müller et al. (the following equation)30:

$${\rm{PIP}}_{25} = \left[ {{\rm{IP}}_{25}} \right]/\left( {\left[ {{\rm{IP}}_{25}} \right] + \left[ {{\rm{Phytoplankton}}\,{\rm{biomarker}}} \right] \times c} \right)$$
(1)

with balance factor c = mean IP25 concentration/mean phytoplankton biomarker concentration.

Due to a presumed terrestrial origin of brassicasterol in this region (see “Results”), the calculation of PIP25 was based on the pelagic algal biomarkers dinosterol.

The tri-unsaturated HBI biomarkers (triene, Z- and E-isomer) are commonly found in marine sediment. Based on studies in the Barents Sea, HBI-III (Z) producers favor nutrient-rich and stratified upper water column at the ice edge53. For simplification, the term “HBI-III” is used in the main text, representing the “HBI-III (Z).” The relationship between the relative proportions of HBI-III (Z) and HBI-III (E) and spring chl a in surface sediment from the western Barents Sea suggests the potential of a novel HBI proxy (HBI TR25) indicating the spring phytoplankton bloom (the following equation)67:

$${\rm{HBI}}\,{\rm{TR}}_{25} = \left[ {{\rm{Z}} - {\rm{triene}}} \right]/\left( {\left[ {{\rm{Z}} - {\rm{triene}}} \right] + \left[ {{\rm{E}} - {\rm{triene}}} \right]} \right).$$
(2)

FC32 1,15 (fractional abundances of C32 1,15-diol) indicative for aquatic riverine input was calculated based on the following equation44:

$${\rm{F}}_{{\rm{C}}32\,1,15} = \left[ {{\rm{C}}_{32}1,15} \right]/\left( {\left[ {{\rm{C}}_{32}1,15} \right] + \left[ {{\rm{C}}_{30}1,15} \right] + \left[ {{\rm{C}}_{30}1,13} \right] + \left[ {{\rm{C}}_{28}1,13} \right]} \right).$$
(3)

For GDGT analyses, 5 g sediment was ultrasonically extracted with DCM:MeOH (2:1 v/v), and internal standard (C46-GDGT, 1 μg per sample) was added prior to analytical treatment. The GDGT fraction was separated from other fractions via open column chromatography and eluted with 5 ml DCM:MeOH (1:1 v/v), dried and re-dissolved in hexane:isopropanol (99:1 v/v), and then filtered via a polytetrafluoroethylene filter with a pore size of 0.45 μm. Compound identification and quantification were carried out by a high-performance liquid chromatography/atmospheric pressure chemical ionization-MS according to the method described by Meyer et al.83. The MS-detector was set for SIM of the following (M + H)+ ions: m/z 1300.3 (OH-GDGT-0), 1298.3 (OH-GDGT-1), 1296.3 (OH-GDGT-2), 1050 (GDGT-IIIa/IIIa′), 1036 (GDGT-IIa/IIa′), 1022 (GDGT-Ia), and 744 (C46 standard), with a dwell time of 57 ms per ion.

Calculation of ring index of hydroxylated tetraethers (RI-OH′) and its derived SST follows Lü et al. (the following equations)32:

$${\rm{RI}} - {\rm{OH}}^{\prime} = \, \left( {\left[ {{\rm{OH}} - {\rm{GDGT}} - 1} \right] + 2 \times \left[ {{\rm{OH}} - {\rm{GDGT}} - 2} \right]} \right) \\ / \left( {\left[ {{\rm{OH}} - {\rm{GDGT}} - 0} \right] + \left[ {{\rm{OH}} - {\rm{GDGT}} - 1} \right] + \left[ {{\rm{OH}} - {\rm{GDGT}} - 2} \right]} \right),$$
(4)
$${\rm{RI}} - {\rm{OH}}^{\prime} = 0.0382 \times {\rm{SST}} + 0.1.$$
(5)

### Accumulation rates

Accumulation rates of bulk sediment, bulk organic carbon, CaCO3, and biomarkers are calculated by using the following equations (cf. Ref. 94):

$${\rm{BulkAR}} = {\rm{SR}} \times \left( {\rm{WBD}} - 1.026 \times {\rm{PO}} \right),$$
(6)
$${\rm{TOC}}\left( /{\rm{CaCO}}_3/{\rm{BM}} \right){\rm{AR}} = {\rm{BulkAR}} \times {\rm{TOC}}\left( /{\rm{CaCO}}_3/{\rm{BM}} \right),$$
(7)

BulkAR = bulk sediment accumulation rate (g cm−2 kyr−1); SR = sedimentation rate (cm kyr−1); WBD = wet bulk density (g cm−3); PO = porosity (%); TOCAR = total organic carbon accumulation rate (g cm−2 kyr−1); CaCO3AR = carbonate accumulation rate (g cm−2 kyr−1); BM = biomarker concentration (μg g−1 Sed); and BMAR = biomarker accumulation rate (μg cm−2 kyr−1).