Amplification of MYCN is the driving oncogenic change in a subset of high-risk neuroblastomas. The MYCN protein and the Aurora-A kinase form a complex during the S phase that stabilizes MYCN. Here we show that MYCN activates Aurora-A on chromatin, which phosphorylates histone H3 at serine 10 in the S phase, promotes the deposition of histone H3.3 and suppresses R-loop formation. Inhibition of Aurora-A induces transcription–replication conflicts and activates ataxia telangiectasia and Rad3-related (ATR) kinase, which limits double-strand break accumulation upon Aurora-A inhibition. Combined inhibition of Aurora-A and ATR kinases induces rampant tumor-specific apoptosis and tumor regression in mouse models of neuroblastoma, leading to permanent eradication in a subset of mice. The therapeutic efficacy is due to both tumor cell-intrinsic and immune cell-mediated mechanisms. We propose that targeting the ability of Aurora-A to resolve transcription–replication conflicts is an effective therapy for MYCN-driven neuroblastoma.
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ChiP-seq and RNA-seq data are available from the Gene Expression Omnibus under accession number GSE144288. Previously published sequencing data that we re-analyzed here are available under accession codes GSM1838910 and GSM1838911 (ref. 74). Source data are provided with this paper. All other data supporting the findings of the study are available from the corresponding authors upon request.
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This work was supported by grants from the European Research council (AuroMYC), German Cancer Aid (Enable), the Federal Ministry of Education and Research (DKTK), the German Research Foundation (via DFG Research Group 2314 to M.E.) and German Cancer Aid (via the Mildred Scheel Early Career Center to G.B.). Cancer Research UK supports the Cancer Imaging Centre at ICR in association with the MRC and Department of Health (England) (C1060/A16464) and a Children with Cancer UK Research Fellowship (to Y.J.). A.G.H. is supported by the German Research Foundation and Wilhelm Sander Stiftung and participates in the BIH-Charité Clinical Scientist Program. E.P. and L.C. received a Children with Cancer UK Project Grant (2014/174). L.C. received Cancer Research UK Program Grants (C34648/A18339 and C34648/A14610). R.B. received a Cancer Research UK Programme Award (C24461/A23302). We thank B. Bauer for technical help with the immunohistochemistry, A. Schlosser for proteomics analyses, W. Schmitz for mass spectrometry, B. Martins da Costa and J. Rolff for help with the animal experiments, S. Heinzlmeier and B. Küster for providing the graphics on Aurora-A inhibitors, and members of the Eilers laboratory for evaluating the immunohistochemical images.
The authors declare no competing interests.
Peer review information Nature Cancer thanks the anonymous reviewers for their contribution to the peer review of this work.
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
a, Propidium-Iodide stained FACS profiles of IMR-5 cells synchronized at the G1/S boundary by a double-thymidine block (T0). Asynchronous cells are shown as controls. Cells were released from the block for 4 h into S phase, for 8 h into G2/M phase and for 14 h into G1 phase Data representative of 3 independent experiments with similar results. b, Fractionation of synchronized MYCN-amplified IMR-5 neuroblastoma cells. (Left): Immunoblots of equal aliquots of each fraction were probed for the indicated proteins. Data representative of 3 independent experiments with similar results. (Right): Quantitation of Aurora-A levels in each cell cycle phase. Shown is mean ± S.D. (n = 3 independent experiments). c, MYCN ChIP at indicated loci in asynchronous IMR-5 cells treated for 4 h with 10058-F4 (100 µM). IgG control was used as control for antibody specificity. Data are presented as mean of technical triplicates. Data representative of 3 independent experiments with similar results. d, Immunoblots of indicated proteins of IMR-5 cells either asynchronous, synchronized by double thymidine block into S or G2 phase or synchronized into mitosis by incubation with nocodazole for 16 h. Data representative of 2 independent experiments with similar results. e, Radar blots documenting specificity of Aurora kinase inhibitors: MLN8237, MK5108, AZD1152; data are from22. f, Quantification of pH3S10 staining in IMR-5 cells treated for 8 h with MLN8237 (1 µM) relative to control (DMSO) cells; each grey dot represents a cell. In S and G2 phase number of spots and in mitotic cells intensity of pH3S10 signal relative to DMSO is shown. Data are presented as mean ± S.D. (n≥614 cells per condition examined over 3 independent experiments). g, Quantification of pH3S10 staining of IMR-5 cells in S phase treated for 8 h with 100 nM MLN8237, 1 µM MK5108 or DMSO as control. Each dot represents one cell. Data are presented as mean ± S.D. (n≥147 cells per condition examined over 3 independent experiments). h, Quantification of pH3T3 intensity in IMR-5 cells treated for 8 h with 100 nM MLN8237, 100 nM AZD1152 or DMSO as control; each grey dot represents a cell. Shown is the mean ± S.D. (n≥47 cells per condition examined over 3 independent experiments). i, Representative example pictures of 3 independent experiments with similar results. White line indicates 15 µm.
a, (Top): Ideogram illustrating the 50 bins with the strongest reduction in pH3S10 MLN8237 as compared to pH3S10 DMSO (blue rectangles). (Bottom): Levels of pH3S10 relative to total H3 along all chromosomes of S phase-synchronized IMR-5 cells treated for 4 h with 1 µM MLN8237 (red line) and DMSO (blue line) (n = 2 independent experiments). b, pH3S10 ChIP at indicated loci in S phase synchronized IMR-5 cells treated for 4 h with 1 µM MLN8237. IgG control was used as control for antibody specificity. Data are presented as mean of technical triplicates Data representative of 3 independent experiments with similar results c, Metagene plot of ChIP-Rx signal for histone H3.3 and pH3S10 in S phase-synchronized IMR-5 cells treated for 4 h with MLN8237 (1 µM) or DMSO. The graph is centered on the first nucleosome (‘+1 dyad’) downstream of the TSS for N = 14,340 genes. (n = 2 independent replicates). d, Browser tracks of MYCN, total RNAPII, H3.3 and pH3S10 of ChIP-Rx at a MYCN high (left) and low (right) bound locus. Nucleosome-free zone is indicated in grey. e, Metagene plot of ChIP-Rx signal for histone H3 and histone H3.3 in S phase-synchronized IMR-5 cells treated for 4 h with 1 µM MLN8237 or DMSO. The signal is centered on the first nucleosome (‘+1 dyad’) located downstream of the TSS for N = 14,340 genes (n = 3 independent experiments). f, Immunoblot of asynchronous SH-EP and SH-EP MYCN cells. Vinculin was used as loading control. Data representative of 3 independent experiments with similar results.
a, Metagene plot of all expressed genes (N = 17,533) illustrating distribution of ChIP-Rx signal of total RNAPII within transcribed regions in S phase-synchronized IMR-5 cells treated for 4 h with 1 µM MLN8237, 1 µM MK5108 or solvent control (DMSO) (n = 2 independent experiments). b, 2D Kernel density plot showing RNAPII traveling ratio in S phase-synchronized cells treated for 4 h with MLN8237 (1 µM) and in control (DMSO) cells (n = 2 independent experiments). c, Metagene plot of ChIP-Rx signal for RNAPII pSer2 in S phase-synchronized IMR-5 cells treated for 2 h with MLN8237 (1 µM), MK5108 (1 µM) or DMSO. The signal is centered on the first nucleosome (‘+1 dyad’) located within 300 nt downstream of the TSS for n = 14,340 genes (n = 2 independent replicates). d, Browser tracks of MYCN, total RNAPII and RNAPII pSer2 of ChIP-Rx at FASN gene. e, Immunoblots of IMR-5 cells that were synchronized for the indicated cell cycle phase and treated with Pladienolide B (PlaB;1 µM), MLN8237 (1 µM), MK5108 (1 µM) or DMSO (4 h). Actin was used as loading control. Data representative of 3 independent experiments with similar results. f, Definition of read categories; blue lines show where reads were mapped to; grey categories (exonic, spliced) reads represent mature mRNA, blue reads categories (exon-intron, intronic) represents non-spliced pre-mRNA, red categories (TSS, TES, TES-RT, intergenic) represents RNA without coding sequence. TSS: Transcription start site, TES: transcription end site. g, Diagram showing the setup of the 4sU-sequencing experiments in S phase-synchronized IMR-5 cells. h, Mean percentage of reads recovered in each category described in (f) (n = 3 independent experiments). All treatments (PlaB, MK, MLN) significantly reduce the percentage of spliced reads relative to the ‘DMSO’ control (p<1.0e-15 using paired two-tailed t test and Wilcoxon matched-pairs signed rank test). i, DRIP using S9.6 antibody after 8 h of 1 µM MLN8237 or 1 µM PlaB treatment. Incubation with RNaseH1 and IgG were used as controls. Shown is the mean of technical triplicates. Data representative of 2 independent experiments with similar results. j, (Left) DRIP using S9.6 antibody after 48 h of siAurora-A treatment. Shown is the mean of technical triplicates. (Right) Immunoblot of siRNA-transfected IMR-5 cells. Vinculin was used as loading control. Data representative of 2 independent experiments with similar results.
a, Quantification of fork progression of SH-EP and SH-EP MYCN cells pretreated for 3 h with 100 nM or 1 µM MLN8237, 1 µM of AZD6738 or a combination (100 nM MLN8237 and 1 µM AZD6738). P-values were calculated using unpaired two-tailed t-test comparing two conditions (n≥168 fibers per condition were examined over 2 independent experiments). b, Annexin-V/PI FACS of IMR-5 or IMR-5 cells with inducible Aurora-A WT and T217D. After pre-treatment for 24 h with doxycycline to induce Aurora-A WT and T217D, cells were treated for 48 h with MLN8237 (100 nM), AZD6738 (1 µM) or both. Shown is the mean ± S.D. (n = 3 independent experiments). c, Annexin-V/PI FACS of IMR-5 treated for 48 h with MLN8237 (100 nM), CHIR-124 (1 µM) or both. Shown is the mean ± S.D. (n = 3 independent experiments). d, DNA-RNA-Immunoprecipitation (DRIP) using S9.6 antibody of IMR-5 cells expressing a doxycycline inducible HA-RNaseH1. After 24 h doxycycline treatment, cells were treated for 8 h with 1 µM MLN8237. Incubation with RNaseH1 and IgG were used as controls for non-specific chromatin binding. Shown is the mean of technical triplicates. Data representative of 2 independent experiments with similar results. Insert shows expression of HA-RNaseH1 in asynchronous IMR-5 cells upon doxycycline treatment for 24 h. Vinculin (VCL) was used as loading control. Data representative of 3 independent experiments with similar results. e, Metagene plot of ChIP-Rx signal for total RNAPII and H3.3 in S phase-synchronized RNaseH1-IMR-5 cells treated for 24 h with doxycycline to induce RNaseH1 expression or EtOH (as control). Data show mean for N = 3,731 genes. The signal is centered on the first nucleosome (‘+1 dyad’) located within 300 nt downstream of the TSS (n = 2 independent experiments). f, Browser tracks of MYCN and total RNAPII of ChIP-Rx in (e) at FASN and NCL gene. g, Metagene plot of ChIP-Rx signal for RNAPII pSer2 in S phase-synchronized RNaseH1-IMR-5 cells treated for 24 h with doxycycline (to induce RNaseH1 expression) or EtOH (as control). Data shown for n = 14,340 genes. The signal is centered on the first nucleosome (‘+1 dyad’) located within 300 nt downstream of the TSS for 14,340 genes (n = 2 independent experiments). h, Quantification of PLA signals between RNAPII and PCNA in asynchronous RNaseH1-IMR-5 cells treated for 24 h with doxycycline to induce RNaseH1 expression. Each dot represents mean PLA signal of all cells in one well compared to solvent control. Shown is the mean ± S.D.. P-value was calculated using unpaired two-tailed t-test relative to DMSO control (n = 3 independent experiments). i, Boxplot showing intensity of pKAP1 staining in mitotic cells upon the treatment with the indicated drugs (8 h) and induction of RNaseH1 expression for 24 h. P-value was calculated using two-tailed t-test between EtOH and Dox of each condition (n≥50 cells per condition were examined over 2 independent experiment).
a, Boxplot showing the concentration of indicated inhibitors in the tumor tissue of mice treated for 24 h or 5 days. N = 4 mice (control, 24 h treatment), 5 mice (5 days treatment). b, Relative changes in body weight of mice treated with MLN8237 and AZD6738 compared to mice treated with vehicle control. Shown is the mean ± S.D. N = 42 mice (control), 43 mice (treated). c, Staining of tumor tissue with S9.6 incubated with different RNases to document specificity of S9.6. staining for R-loops (n = 1 section for each experimental condition). d, Histology of proliferative gut tissue in untreated (top) and treated (bottom) mice showing H&E, Ki-67 and cleaved caspase 3 staining. N = 2 mice (control), 3 mice treated). e, Representative MRI as well as sections of a tumor treated with a combination of MLN8237 and olaparib (N = 3 animals were evaluated). f, MRI sections of the two long term-surviving mice at day 0 and after 7 days of treatment with the combination of AZD6738 (30 mg/kg) and MLN8237 (15 mg/kg). Dashed white lines indicate tumor circumference. g, P-values calculated using Mantel-Cox log-rank test comparing the survival of different groups shown in Figure 6c.
a, Histology of representative tumor sections of MYCN-amplified PDX models treated as indicated. AZD6738 was administered at 50 mg/kg every day and MLN8237 at 7.5 mg/kg on a 5 days on, 2 days off schedule. Shown are stainings from tumors recovered after treatment for 14 days. Each group comprises three animals. b, Box plot showing quantification of R-loop-, γH2AX- and cleaved caspase 3-positive cells in tumor sections. P-values were calculated using unpaired two-tailed t-test using Welch’s correction (n≥9 sections from animals described in panel (a) were evaluated). c, Relative changes in tumor volume of four MYCN non-amplified PDX models upon treatment with the indicated inhibitors. Shown is the mean ± S.E.M. N indicates the animal number for each experimental cohort. P-values comparing control and combination (indicated with a black dashed line) were calculated using unpaired two-sided t-test.
a, GSEA signatures showing response of a hallmark gene set indicating Interferon gamma response in the TH-MYCN (top) and a PDX (bottom) model. b, (Top): Representative pictures by flow cytometry showing cell gating. (Bottom): Populations of CD45+ immune cells in the tumor microenvironment after treatment of TH-MYCN mice with the combination of AZD6738 (25 mg/kg) and MLN8237. Shown is the mean ± S.E.M.. Significance was calculated using unpaired two-tailed t-test (N = 4 animals from each condition were evaluated). c, Histology of representative tumor sections showing NKp46-positive cells and pSTAT1 in tumors of TH-MYCN mice treated with combined Aurora-A/ATR inhibition. N = 4 mice (control, 24 h treatment), 5 mice (5 days treatment). d, Relative changes in tumor volume of subcutaneous xenografts in nude mice after treatment with vehicle or the combination of MLN8237 and AZD6738 (25 mg/kg). Shown is the mean ± S.E.M. (N = 5 animals per group). e, Bar graph showing the survival of allograft mice treated with MLN8237 and AZD6738. Shown is the mean ± S.E.M. (N = 4 animals per group).
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Roeschert, I., Poon, E., Henssen, A.G. et al. Combined inhibition of Aurora-A and ATR kinases results in regression of MYCN-amplified neuroblastoma. Nat Cancer 2, 312–326 (2021). https://doi.org/10.1038/s43018-020-00171-8
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