Abstract
This paper describes the experimental determination of concentration factors (CF) for nickel, ruthenium and antimony in the model diatom Phaeodactylum tricornutum Bohlin (Bacillariophyceae), which was chosen as a representative of marine phytoplankton. Better determinations of these CF are needed to improve the modelling of marine ecosystems at release points, where radioactive pollutants enter the ecosystem, for more accurate predictions of radiation dose to humans caused by these pollutants. A literature study revealed that the currently implemented values of these CF are based on very scarce data, and a computational sensitivity study showed that the radiation dose caused by radioisotopes of these elements depend strongly on the phytoplankton CF. Nutrient-enriched water samples from Swedish coastal waters were used as a medium for growing of the diatom species P. tricornutum and radioactive isotopes of the studied elements were added to the cultures during the exponential growth phase. The radioactivity in the P. tricornutum and in the culture medium were measured separately and used for determination of CF. Conservative estimates of the CF based on this phytoplankton proxy on the present data are 6400 L/kg for nickel, 20,000 L/kg for ruthenium and 890 L/kg for antimony, with P. tricornutum biomass masses referring to dry weight. The estimates for nickel and ruthenium are similar to previously published values, which underpins the credibility of radiation dose calculations based on these values. The estimate for antimony is uncertain, but also, to our knowledge, represents the first published experimentally based data on this CF.
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Introduction
Radionuclides are among the various contaminants released into marine ecosystems through discharges linked to human activities, most predominantly from the nuclear industry, both during normal operations and from unforeseen releases such as nuclear reactor incidents and accidents1, 2. The assessment of the resulting radiation dose to various recipients requires monitoring of the releases and studies on how the radionuclides are taken up by various organisms within food webs. Since nuclear power reactors are frequently situated on coastal areas, the marine ecosystems are often the recipients of radionuclide releases, and hence the transport of radionuclides within these ecosystems must be studied. In the marine environment, estimates of release and uptake of radionuclides are mostly based on simulation models with limited experimental data (e.g. PREDO1 and Erica-tool2). A common parameter used in these models is the concentration factor (CF), which relates the concentration of an element in an organism to the concentration in the medium under equilibrium conditions.
Swedish nuclear facilities perform assessments of the radiation dose to the public using the software PREDO (PREdiction of DOses from normal releases of radionuclides to the environment1). In PREDO, a marine food web model is implemented, in which phytoplankton are at the base of the food chain. Thus, phytoplankton CF are expected to be important for estimates of internal radiation dose from radionuclides which reach humans through the food chain. The dependence of the estimated radiation dose on the phytoplankton CF for radioisotopes of the studied elements was studied in this work, to quantify this importance.
A previous screening using PREDO, based on actual releases from Swedish nuclear facilities to the marine environment3, resulted in a list of 15 radionuclides which are together responsible for more than 99% of the calculated radiation dose to humans. Literature studies of phytoplankton CF for the corresponding elements showed that most of them had already been subject to extensive studies. The determination of phytoplankton CF for two of the less well studied elements (Mn and Zn) is reported in previous work by the authors4. Furthermore, two isotopes of antimony (124Sb and 125Sb) were on the list. Calculations performed by the nuclear power plants for different realistic scenarios indicate that isotopes of ruthenium and nickel could also be among the important contributors to the dose to the public.
The literature was searched for experimentally derived data on the set of elements which were thus deemed to be of radioecological relevance, and these literature studies show that the phytoplankton CF for nickel, ruthenium and antimony are not well established.
Publications by the International Atomic Energy Agency (IAEA)5,6,7 and the Wildlife Transfer Database8 cover most of the literature on concentration factors. In addition to screening these documents, a literature search was performed using Web of Science (core collection) with the search phrase “[element] AND “phytoplankton” AND (“concentration factor” OR “concentration ratio” OR bioaccumulation OR bioconcentration)”. No further relevant publications or data was found for nickel, ruthenium or antimony, so it was concluded that the IAEA technical documents cover all the published literature on CF for the studied elements. The values recommended in the three latest relevant IAEA technical documents are listed in Table 1, along with references to the primary sources when available. The CF value for nickel in IAEA TECDOC 4226 is calculated from nickel concentrations from Refs.9,10,11, i.e., these references do not state CF data. It is noted that the values stated in the oldest relevant IAEA publication (IAEA TECDOC 2115) are generally identical, information on their primary source and experimental conditions is lacking, and no reference to whether fresh or dry weight of phytoplankton biomass was used in the calculations of these values is provided. The reference for the phytoplankton CF value for antimony in IAEA TECDOC 4226 is IAEA TECDOC 2115, i.e. also lacking information on primary source and statement on fresh/dry weight. No phytoplankton CF value is available for antimony in IAEA TECDOC 4797 and the values for nickel and ruthenium are very widely spread, with standard deviations exceeding the arithmetic mean value.
In conclusion, there is very little data on phytoplankton CF available for the three elements nickel, ruthenium and antimony, while they are nevertheless relevant for realistic dose predictions. Hence, this work aims to complement the scarce data through experimental determination of these CF in a representative model organism. In addition, we aim to quantify any differences in the studied CF between the two main marine environments around the Swedish coasts, i.e. saline and brackish waters.
The diatom Phaeodactylum tricornutum Bohlin (Bacillariophyceae) was chosen as model organism for the study. Diatoms are by far the largest group of phytoplankton in the seas around Sweden12, and P. tricornutum is a useful representative of the group in this context since it is a cosmopolitan diatom that thrives equally well in saline and brackish waters13.
Materials and methods
Computational sensitivity studies
The aquatic model in the software PREDO3 was used to assess to which extent the committed dose from each of the investigated radionuclides is a result of exposure through the marine food chain. In PREDO, the marine ecosystem is represented by a box model including radionuclide transfer between different geographical regions, from the water column to marine biota and between different trophic levels within the food web (as described in, e.g.,18) ultimately leading to exposure of humans through intake of food of marine origin. Other marine exposure paths, such as swimming and inhalation of sea spray, are also included in the model. These exposure paths are independent of the food chain and thus independent of the CFs. The transformation of radionuclides to other nuclides through radioactive decay is also included in the model.
The PREDO model for the marine environment at the Ringhals nuclear power plant (NPP) located on the west coast of Sweden (see Fig. 1) was used. The annual dose after 100 years of release of 1 Bq/year is calculated and then normalized to actual releases. This dose was calculated for adults, children and infants of four different human population groups (fishing, hunting, farming and vegetarian families), according to the assumptions on habits and diet for the different families19. The implemented CF for each of the studied elements (Ni, Ru and Sb) was varied by a factor 10 and 1/10 respectively, to assess to which extent the dose is proportional to the CF, i.e. that exposure stems primarily from the marine food chain. The dose was only calculated for one nuclide of each of the elements (63Ni, 106Ru and 125Sb) since the same CF applies to all isotopes.
Seawater sampling and analysis
Seawater samples were collected to be used as a growing medium for the experiment with a composition representative of the studied marine environments. The Swedish Meteorological and Hydrological Institute (SMHI) collected seawater samples in February 2022, at the regular sampling stations Anholt E (Kattegat, 56° 40.11 N, 12° 06.63ʹ E, sampling depth 50 m, water depth 63 m, salinity 33‰) and Karlsödjupet (Baltic sea—57° 07.03ʹ N, 17° 40.12ʹ E, sampling depth 80 m, water depth 110 m, salinity 10‰)20, see Fig. 1. The sampling stations in question were chosen because of their proximity to the Ringhals and Oskarshamn NPP, and because they represent the two main marine environments surrounding Sweden (saline and brackish water). The origin of the seawater used in the following experiments, i.e. whether the seawater comes from the Anholt E or Karlsödjupet sampling station, will henceforth be referred to as “water origin”.
The samples were collected using a water sampler (Hydro-Bios Free Flow Water Sampler) and immediately poured into 5L cans (Fisherbrand™ Polyethylene Narrow Neck Jerry Can) and refrigerated under dark conditions. Sampling was carried out according to methods recommended within the HELCOM COMBINE program21. The cans were previously unused and rinsed thrice with ultrapure (Milli-Q ®) water prior to use.
Natural background concentrations of Ni, Ru and Sb in the water samples used as growing medium were determined, primarily to assess to which extent the added element concentrations would disturb the natural concentrations. The concentrations were measured by ICP-MS (Thermo iCAP Q) using the standard addition method22. The values were corrected for instrumental background. Standards of 0, 1, 5 and 10 ppb of the respective elements were prepared from 10 ppm stock solutions (UltraScientific, CPA Chem, VGA Labs). Seawater samples were divided in four sub-samples that were diluted 1.7 times with 0.5 M HNO3 (Suprapur Merck) containing one standard for each sub-sample and internal standards of 2 ppb Sc, In and Bi. Mass intensity readings at masses 60, 104 and 121 were taken for Ni, Ru and Sb, respectively. Natural isotopic composition was assumed for the calculation of total element concentrations. The detection limit and accuracy of the method depend on concentration and the atomic weight of the element in question, and the accuracy (1σ) for each measurement is stated separately in the Results section.
Natural background concentrations of gamma-emitting radionuclides in the water samples was assessed by a 3-day measurement on 1.5 L of water from each water origin, using a detector system consisting of a high-purity germanium coaxial detector (Ortec GEM 50P4, Ametek, USA) with a relative efficiency of 52%, and a digital signal processor (DSPEC jr 2.0, Ametek, USA) providing an energy resolution of 1.65 keV at 1.33 MeV. Expected background concentrations of naturally occurring radioactive elements and 137Cs pollution remaining from the Chernobyl accident were detected, but no background radioactivity corresponding to the currently studied radionuclides. Natural background concentrations of beta-emitting radionuclides were assessed by a 10 min measurement on 5 mL of water from each water origin using a Liquid Scintillation Counter (Packard Tri-Carb® 2100TR, Perkin-Elmer, USA) with an energy range of 0–2000 keV, and 15 mL of liquid scintillation cocktail (Ultima Gold™, Perkin-Elmer, USA), mixed in a polyethylene scintillation vial (Selecta). No background concentration of any beta-emitting radionuclides was detected.
P. tricornutum cultures
A non-axenic strain of P. tricornutum was procured from the algal bank at the University of Gothenburg (https://www.gu.se/en/marina-vetenskaper/about-us/algal-bank-gumacc). The bacteria were approximately one attached bacterial cell per 10 algal cells in a stationary culture (quantified by manual counting under microscope). No free-living bacteria were observed. The bacteria’s contribution to the total biomass was assumed to be negligible, due to their small size and that the measured algal cell dry weight was found to agree well with literature values. The P. tricornutum were cultured in nutrient-enriched, sterile filtered (not autoclaved) seawater samples, contained in 50 mL polystyrene cell culture flasks with vented caps (Thermo Scientific™ Nunc™ EasYFlask™, Nunclon™ Delta surface plasma treated). The culture flasks were previously unused and rinsed thrice with ultrapure (Milli-Q ®) water prior to use.
Sterile filtering was performed directly into the cell culture flasks, immediately before starting the cultures, using 0.2 μm polycarbonate membrane filters (Cytiva Whatman™ Cyclopore™). The nutrients (8.8‧10–4 M NaNO3, 3.6‧10–5 M NaH2PO4 and 1.1‧10–4 M Na2SiO3) were added to the sterile filtered seawater in the proportions of the f/2 medium23, however without vitamins or trace elements. Trace elements and vitamins were avoided in order to keep the water composition as representative as possible for the in-situ conditions in the marine environment. Preliminary experiments showed that excluding the trace elements and vitamins still yielded a satisfying growth rate of the P. tricornutum. Addition of nutrients was necessary to ensure exponential growth and is common practice in trace element metabolism experiments4, 24,25,26. The filtering funnels were washed with hot water and soap for several hours and thereafter rinsed thrice with distilled water before re-use; all other filtering equipment was single-use.
The radionuclides were added as solutions of NiCl2, RuCl3 and SbCl3 respectively. The Ni solution (PerkinElmer Life and Analytical Sciences, Boston, MA, USA) had an activity concentration of 60 MBq/L (± 10%) and a carrier concentration of 7.0 Nitotal/63Ni. The RuCl3 and SbCl3 solutions (Eckert & Ziegler Strahlen- und Medizintechnik AG, Germany) both had an activity concentration of 500 GBq/L (± 15%) and inactive carrier concentrations of 790 Rutotal/106Ru and 130,000 Sbtotal/125Sb respectively. The added radionuclide concentrations and the added stable carrier concentrations for the respective solutions are listed in Table 2, together with the results of the seawater analysis and the final total element concentrations, i.e. the sum of the added and measured element concentrations. The ruthenium and antimony solutions were strongly acidic (6 M HCl), so after addition to the seawater, NaOH solution was added to restore pH to 8 as it was before the radionuclide additions. The pH was monitored by the use of pH indicator sticks (Fisherbrand®, pH Indicator Paper Sticks, pHix 0–14) and remained constantly at 8 ± 0.5 throughout the experiment.
After addition of nutrients and radionuclides and, where necessary, adjustment of pH, the culture growth was started by adding 1 mL of stock P. tricornutum culture to 29 mL of the solution. The resulting initial P. tricornutum cell concentration in the cultures was approximately 25‧103 cells/mL. The stock solution composition and background element concentration in the initial phytoplankton population (approximately 1/40 to the final population) were deemed to be negligible and were thus not quantified. Three separate cultures were grown for each radionuclide and water origin (18 samples in total), for statistical purposes.
To provide a control for the experiment, identical control samples (also 18 in total) with the same water origins, nutrients and radionuclide additions, but with no P. tricornutum, were prepared. These control samples were used for assessing the amount of radioactivity sorbed onto the filters later used for separating the P. tricornutum from the medium.
The cultures and control samples were subjected to controlled light conditions; cold-white (6000 K) LED illumination was provided at an intensity of 200 µEm-2 s−1, similar to what has been used in previous experiments4, 25, 27 and (at the surface) slightly above optimal conditions for phytoplankton growth28. Illumination was provided 12 h/day, representative of the average daylight period. The temperature varied slightly, from 20 °C at the beginning of the light period to 25 °C at the end of the light period. These variations were caused by the illumination, but were deemed unimportant for the results, since the metabolism of P. tricornutum has been shown to be relatively insensitive to temperature fluctuations in this interval13. In preliminary experiments, constant gentle shaking was applied, which however affected neither the growth rate nor the metal uptake. In the reported experiments, the culture flasks were not shaken, except for a gentle swirling when the growth was assessed, at two-day intervals.
An Automated Cell Counter (Countess, ThermoFisher Scientific) was used for assessing P. tricornutum concentration and growth. A 100 µL aliquot was collected from each culture flask and fixated with 2 µL of acidic Lugol’s solution for P. tricornutum counting. This was performed at approximately two-day intervals, until a P. tricornutum concentration of approximately 106 cells/mL was reached, generally within 6–8 days.
Concentration factor determination
The entire 30 mL samples were filtered when the desired P. tricornutum concentration had been reached (i.e. 6–8 days after inoculation), and the radioactivity of the different fractions was measured, whereafter CFs were calculated using
where Vmedium and Amedium are the volume and the activity of the filtrate (the growing medium) respectively, Aplankton is the activity of the filters with P. tricornutum, Acontrol is the activity on the filters used for the control samples and mplankton is the total P. tricornutum dry mass.
Dry weight determination
The dry weight of the P. tricornutum was determined by filtering 25 separate P. tricornutum cultures (13 with Anholt water and 12 with Karlsödjupet water) with known volume and cell concentration through polycarbonate membrane filters (Cytiva Whatman™ Cyclopore™, pore size 1 µm, diameter 25 mm) which had been pre-soaked in NH4HCO2 (Ammonium formate, Acros Organics, 99%) solution (isotonic with the sea water), dried and weighed.
After P. tricornutum culture filtration with a transmembrane pressure of 17 kPa, the filters and P. tricornutum were rinsed twice with 5 mL of the isotonic NH4HCO2 solution. Rinsing was performed to avoid adding salt mass to the filters, NH4HCO2 solution was used since it evaporates completely at 60 °C, and the solution was prepared to be isotonic with the sea water to avoid osmotic rupture or shrinking of the rinsed plankton. After rinsing, the filters were dried overnight at 60 °C and immediately weighed again. The dry weight of a single P. tricornutum cell was calculated by dividing the filter mass gain by the number of cells25.
For the concentration factor determination, the total P. tricornutum dry mass in each of the experimental samples was calculated as the product of the dry weight per cell and the number of cells in the respective sample as assessed by automated cell counting.
Radioactivity measurements
For the activity measurements, the samples with added radionuclides (both P. tricornutum samples and control samples) were filtered through polycarbonate membrane filters (Cytiva Whatman™ Cyclopore™, pore size 1 µm, diameter 25 mm) with a transmembrane pressure of 17 kPa. The filters were then rinsed twice with 5 mL of sterile filtered seawater before activity measurements.
Great care was taken to prevent dust particles from falling into the flasks, onto which the studied elements might possibly adsorb. Such particle contamination would lead to a disproportionately high measured radioactivity in the fraction caught on the filters. However, the risk could not be entirely eliminated.
The activity measurements on the filters and the filtrates were performed differently for the different radionuclides, due to their different decay characteristics.
The activity of 125Sb could be assessed directly by gamma counting, using a detector system consisting of a high-purity germanium coaxial detector (Ortec GEM 50P4, Ametek, USA) with a relative efficiency of 52%, and a digital signal processor (DSPEC jr 2.0, Ametek, USA) providing an energy resolution of 1.65 keV at 1.33 MeV. The counting time was chosen to ensure a counting uncertainty less than 3% (1σ). Geometric efficiency corrections were made using the EFFTRAN v. 4.2 computer code29 and previous measurements of a calibration source.
106Ru (t1/2 = 371.5 d) decays by beta emission only to 106Rh (t1/2 = 30.1 s), which in turn decays by beta emission to stable 106Pd. The latter decay is accompanied by gamma emissions, which enables the indirect detection of 106Ru decay through gamma spectrometry30. The relatively short half-life of 106Rh means that a secular equilibrium is established already within minutes, i.e. the decay rate of 106Ru and 106Rh are then identical. Thus, the activity of 106Rh was measured using the same detector system as for 125Sb and the counting time was chosen to ensure a counting uncertainty less than 3% (1σ).
The activity adsorbed in the culture flasks was measured for 25 flasks, by placing each culture flask on the detector and, for interpreting the results, making suitable geometry corrections using EFFTRAN v. 4.229. Also for the flasks, the counting time was chosen to ensure a counting uncertainty less than 3% (1σ).
63Ni is also a beta emitter only, which decays directly to stable 63Cu with no accompanying gamma emissions. Hence, the activity of 63Ni in the water and P. tricornutum fractions were measured by liquid scintillation counting using a Liquid Scintillation Counter (Packard Tri-Carb® 2100TR, Perkin-Elmer, USA) with an energy range of 0–2000 keV, a liquid scintillation cocktail (Ultima Gold™, Perkin-Elmer, USA) and polyethylene scintillation vials (Selecta). For water samples, 5 mL of the water was mixed with 15 mL of the scintillation cocktail and for measurement of activity on filters with P. tricornutum and control filters, the entire filter was immersed in 20 mL scintillation cocktail and shaken well for 30 s. Visual inspection confirmed that all but a negligible fraction of the P. tricornutum was dispersed in the scintillation cocktail. Quenching corrections were performed by adding a known amount of activity to a separate set of vials containing similar water/cocktail, filter/cocktail and P. tricornutum /filter/cocktail mixtures and measuring the corresponding count rates. Direct proportionality of the count rate to the activity was confirmed by count rate measurements for a range of known activities, spanning the range of measured activities in the different samples. Counting was carried out for 2 min for each sample, and the count rate at energies below 67 keV were used for the activity calculations. Six repeated measurements on the same sample showed an uncertainty of 1.4% (1σ).
Results and discussion
Computational sensitivity studies
The effective doses received during 1 year for three age groups and four population groups were calculated using PREDO for 63Ni (Fig. 2a), 106Ru (Fig. 2b) and 125Sb (Fig. 2c). The definitions of the age categories and population groups are given in reference19. As previously stated, the values are calculated assuming a hypothetical continuous release of 1 Bq/year for 100 years and are thus only intended for intercomparison. For all studied population groups and elements, the calculated dose was higher for lower age, which is expected due to the age dependence of the used dose coefficients1 caused by differences in biokinetics between different age groups31. In general, the fishing family received the highest dose, for most studied age categories and elements, with some exceptions for nickel. For ruthenium, the calculated dose is significantly lower for the vegetarian family compared with the other families, and the dose for the other families is also almost directly proportional to the ruthenium CF, indicating that the dose from radioisotopes of ruthenium arises almost exclusively by ingestion. For antimony, the difference between the vegetarian and other families is less pronounced, whereas for nickel, the CF is less important for the calculated dose, indicating more exposure through other paths than the marine food chain. At the currently implemented value of the nickel CF, 1000 L/kg, the dose received by the farmer child is almost 3 times higher than that of the vegetarian child, so the ingestion exposure pathway is still dominant, indicating that for nickel, as well as for ruthenium and antimony, the CF is essential for calculation of radiation dose to all non-vegetarian population groups.
Element concentrations in seawater samples
Results of the ICP-MS analysis of the concentrations of nickel, ruthenium and antimony in the seawater samples are listed in Table 2.
The measured nickel concentration is almost an order of magnitude larger than concentrations previously found in Kattegat and the Baltic Sea (5–14 nM)32, which could be due to contamination during sampling or storage33, or to increased pollution since these values were obtained in 1982. Seawater samples retrieved in February 2021 by the same sampling methodology, in the same locations (which were however not used in this experiment) showed even higher concentrations (134 nM). Even if the measured concentrations are due to sample contamination, and the true Ni concentrations are as low as reported in 198232, the added Ni concentration (2 nM) does not alter the total Ni concentration in the seawater samples to a large extent, so the CF values obtained in the reported experiments are likely to be representative for the in-situ conditions in the studied marine environments.
Data on ruthenium concentrations in Kattegat or the Baltic Sea could not be found. The ruthenium concentration in heavily trafficked waters in the Mediterranean Sea has been measured to be 0.5 nM34, which is much lower than our measured values. However, ruthenium pollution occurs to an important extent through wear of ruthenium containing alloys which are frequently used in high-wear applications34, resulting in small ruthenium-containing particles which may have been included in our analysis, but not included in34 due to finer filtering. Other possibilities include contamination of the samples or an actual much higher ruthenium pollution in the studied marine environments. Irrespective of the actual value, the addition of 160 nM ruthenium in the reported experiments results in a significant increase of the ruthenium concentration in the used growing medium. If ruthenium uptake in P. tricornutum is concentration dependent or even reaches a saturation level, the obtained CF values are thus conservative. The ruthenium content was not assessed in the samples taken in February 2021.
The antimony concentration in the Baltic Sea has been measured to be 0.3–0.8 nM35 and 1.7 nM in the North Atlantic36 which is similar to our measured data, and to the antimony concentration found in the water samples retrieved in February 2021 (0.7 nM). Similarly to the ruthenium case, the concentration of antimony added as radionuclide and stable carrier results in a significant increase of the total concentration above the background, so that the obtained CF values are conservative if the CF would be concentration dependent.
P. tricornutum dry weight
The dry weight of one P. tricornutum cell was found to be 23 ± 5 pg (1σ). No statistically significant difference was found between the dry weight of P. tricornutum grown in water from Anholt E or Karlsödjupet respectively, so the same value was used in the concentration factor calculation for water from both origins. The found value agrees well with previously published values; 22.6 pg per cell26 and 23 pg organic mass per cell37.
Concentration factors
A CF was calculated individually for each sample according to Eq. (1), where mplankton was calculated as the product of the plankton cell concentration, the culture volume and the P. tricornutum cell dry weight. The final P. tricornutum cell concentration at the time of filtering was 1.1‧106 ± 0.4‧106 cells/mL. For each element and water origin, a mean value and standard deviation was calculated from the three CF thus obtained. These values are listed in Table 3. For nickel, the mean activity measured on the control filters was less than 1% of that caught on filters with P. tricornutum. For ruthenium and antimony, the corresponding number was 25% and 21% respectively. Given that the P. tricornutum were in a state of exponential growth, the obtained values are conservative estimates of the CF.
For the gamma emitting nuclides, 106Ru and 125Sb, the activity remaining in the culture flasks was ≤ 3.3% of the total recovered activity, except for the samples with P. tricornutum growing in water from Karlsödjupet with an addition of 106Ru, in which case the activity remaining in the culture flask was, on average, 15%. The recovered radioactivity (i.e. the radioactivity detected in the medium, P. tricornutum and flasks) was equal to the added radioactivity within the uncertainty of the radioactivity concentration of the stock solution, which was 15%, as given by the radionuclide provider. Due to the difficulty of measuring the activity of the culture flasks with the scintillation detector, which would have demanded the flask to be cut in small pieces and introduced in a scintillation vial, the activity remaining in the flasks where P. tricornutum had been cultured with an addition of 63Ni was not measured. The average recovered activity of 63Ni in the medium and P. tricornutum fractions was 91%. Furthermore, since the measured radioactivity in the P. tricornutum filters, the control filters and the medium were used relatively to each other, absolute radioactivity quantities are not important for the CF determination. Although sorption on the walls occurs, this does not affect the CF determination. Due to the dynamic equilibria in the sorption process, the distribution of radionuclides between the P. tricornutum and the surrounding medium will be unaffected by sorption on the walls.
Nickel
The difference between the CFs calculated for the water samples from Anholt E and Karlsödjupet respectively is not statistically significant (the difference is smaller than one standard deviation), which suggests that the nickel CF is not concentration dependent within the relevant nickel concentration range, since the Ni concentration at Anholt E is approximately twice that at Karlsödjupet. Both values (1200 L/kg and 1100 L/kg fresh weight, respectively) are also, within one standard deviation, identical to the geometric mean of the values listed by IAEA7 (570 ± 740 L/kg fresh weight).
Ruthenium
The calculated CFs for ruthenium are also, within one standard deviation, identical for the two water sampling sites. The total ruthenium concentration differed by 18% between the media based on Anholt E and Karlsödjupet water respectively. The absence of a statistically significant difference thus suggests that the CF is at least not strongly concentration dependent, and there are no signs of saturation effects, at the comparatively high ruthenium concentrations present in the experiment. Since the addition of ruthenium was large compared with the natural background concentrations, this conclusion may not hold for the lower ruthenium concentrations found in the studied marine environments, but it is unlikely that there would be a concentration dependence at low concentrations but not at higher. The values, 2700 L/kg and 3600 L/kg fresh weight, for Anholt E and Karlsödjupet respectively, are both within one standard deviation from the mean value given by IAEA TECDOC 4797 (6700 ± 8500 L/kg fresh weight). It should be mentioned that the very high value recommended in IAEA TECDOC 4226 (200,000 L/kg fresh weight), which is currently implemented in PREDO, refers to16 who in turn refer to a conference paper from 196538 where it is explicitly mentioned that the data, established for Caribbean ecosystems, are preliminary. Thus, our results are very likely to represent a better estimate of the typical phytoplankton CF in Swedish marine ecosystems.
Antimony
Finally, the CF values for antimony calculated from our measurement data are quite widely spread. For one of the samples with water from Karlsödjupet, the measured activity in the P. tricornutum fraction was an order of magnitude larger than for the other two samples, although the P. tricornutum concentration was similar. We assume that this was due to some foreign particle(s) entering the culture, onto which much antimony was adsorbed. This sample was therefore excluded from the analysis. The mean value for Anholt E (34 L/kg fresh weight) has an 81% RSD and the mean value for Karlsödjupet (160 L/kg fresh weight) is more than two standard deviations larger, however, given that no standard deviation could be calculated for the Karlsödjupet value, it is not possible to determine if the difference is statistically significant. No value is given in IAEA TECDOC 4797, while the recommended value in IAEA TECDOC 4226 (1000 L/kg) is the same as in IAEA TECDOC 2115, for which no original source is stated. Furthermore, no information is given on how the value was obtained or whether it refers to phytoplankton fresh or dry weight. Thus, our values probably represent the first published CF measurement data for antimony in phytoplankton.
Conclusions
The results from the computational sensitivity study with the marine ecosystem model in PREDO showed that correct CFs for phytoplankton are important for a correct dose assessment for the different population groups, except for vegetarians for which other sources than seafood ingestion dominated the dose.
Conservative estimates of the fresh weight CFs in the marine diatom species P. tricornutum are 6400 ± 1900 L/kg dry weight for nickel, 20,000 ± 8000 L/kg dry weight for ruthenium and 890 L/kg dry weight for antimony (where no standard deviation could be calculated). Differences between the CF between waters of different origin are, to the precision of our measurements, not significant, indicating that the same CF can be used for all dose estimates concerning Swedish marine environments.
The estimates for nickel and antimony do not deviate strongly from the values of these CF which have been published previously and implemented in PREDO, so this work serves to reinforce the scientific basis of the phytoplankton CF used for assessments of dose to the Swedish public from radioactive isotopes of nickel and antimony. The phytoplankton CF estimate for ruthenium is similar to the value given in the latest relevant IAEA report on CF7, but significantly lower than that currently implemented in PREDO, so this work reinforces the scientific basis for abandoning the currently implemented ruthenium phytoplankton CF of 200,000 L/kg fresh weight for a lower value, which should ultimately result in more accurate estimates of radiation dose to the Swedish public from radioactive isotopes of ruthenium.
Data availability
Data is available upon reasonable request to the corresponding author.
References
Vattenfall. PREDO—PREdiction of DOses from normal releases of radionuclides to the environment. Introduction, QP.50000-63714825 v2.0, Vattenfall AB, Stockholm, (2015).
Beresford, N.A., Brown, J., Copplestone, D., Garnier-Laplace, J., Howard, B.J., Larsson, C.-M., Oughton, D., Prohl, G., & Zinger, I. An integrated approach to the assessment and management of environmental risk from ionising radiation: description of purpose, methodology and application. ERICA Deliverable: D-ERICA. EC Contract No FI6R-CT-2004-508847 (2007)
Vattenfall. PREDO—PREdiction of DOses from normal releases of radionuclides to the environment. Methodology for the assessment of radioactive releases to the aquatic environment, QP.50000–63747891 v. 2.0, Vattenfall AB, Stockholm, (2015).
Insulander Björk, K., Thomas, R., Holgersson, S. & Isaksson, M. Experimental determination of concentration factors of Mn, Zn and I in the phytoplankton species Phaeodactylum Tricornutum. J. Environ. Radioact. 261, 107134 (2023).
IAEA The radiological basis of the IAEA revised definition and recommendations concerning high-level radioactive waste unsuitable for dumping at sea, Technical Report No. 211, IAEA, Vienna (1978).
IAEA Sediment Distribution Coefficients and Concentration Factors for Biota in the Marine Environment, Technical Report No. 422, IAEA, Vienna (2004).
IAEA Handbook of Parameter Values for the Prediction of Radionuclide Transfer to Wildlife, Technical Report No. 479, IAEA, Vienna (2014).
Copplestone, D., Beresford, N.A., Brown. J.E., Yankovich, T. An international database of radionuclide concentration ratios for wildlife: Development and uses. J. Environ. Radioact. 126, 288-298 (2013).
Martin, J. H. & Knauer, G. A. The elemental composition of plankton. Geochim. Cosmochim. Acta 37, 1639 (1973).
Laevastu, T. & Thompson, T. G. The determination and occurrence of nickel in seawater, marine organisms, and sediments. J. Cons. Int. Explor. Mer 21, 125 (1956).
Eisler, R. Trace metal concentrations in marine organisms (Pergamon Press, 1981).
SIME - Swedish Institute for the Marine Environment. Sveriges vattenmiljö, tillståndsrapport växtplankton. https://www.sverigesvattenmiljo.se/sa-mar-vara-vatten/2019/variabelgrupper/85/12/62, retrieved 2020–03–17 (2020).
Kräbs, G. & Büchel, C. Temperature and salinity tolerances of geographically separated Phaeodactylum tricornutum Böhlin strains: maximum quantum yield of primary photochemistry, pigmentation, proline content and growth. Bot. Mar. 54(3), 231–241 (2011).
Ancellin, J., Guegueniat, P. & Germaine, P. Radioecologie marine (Eyrolles, 1979).
Bowen, H. J. M. Environmental chemistry of the elements (Academic Press, 1979).
Lowman, F.G., Rice, T.R., & Richards, F.A. Accumulation and redistribution of radionuclides by marine organisms, Radioactivity in the Marine Environment, National Academy of Sciences,Washington, DC 161 (1971).
Kimura, Y., Ogawa, Y., Honda, Y. & Katsurayama, K. Japanese J. of. Health Phys. 25(4), 361–368 (1990).
Saremi, S., Isaksson, M. & Harding, K. C. Bio accumulation of radioactive caesium in marine mammals in the Baltic Sea—Reconstruction of a historical time series. Sci. Total Environ. 631–632, 7–12 (2018).
Vattenfall PREDO—PREdiction of DOses from normal releases of radionuclides to the environment. Representative person. QP.50000–63744910 Vattenfall AB, Stockholm, (2015).
SMHI - The Swedish Meteorological and Hydrological Institute. SHARKweb. https://sharkweb.smhi.se/hamta-data/, retrieved 2022–09–09 (2022).
HELCOM “Manual for marine monitoring in the COMBINE programme of HELCOM” https://helcom.fi/action-areas/monitoring-and-assessment/monitoring-guidelines/combine-manual/ (2017).
Christian, G. D. & O’Reilly, J. E. Instrumental analysis (Allyn and Bacon Inc., 1986).
Guillard R.R.L. Culture of Phytoplankton for Feeding Marine Invertebrates. In: Smith W.L., Chanley M.H. (eds) Culture of Marine Invertebrate Animals. Springer, Boston, MA. (1975).
IAEA Design of Radiotracer Experiments in Marine Biological Systems, Technical Report No. 167 IAEA, Vienna (1975).
Heldal, H. E., Stupakoff, I. & Fisher, N. S. Bioaccumulation of 137Cs and 57Co by five marine phytoplankton species. J. Environ. Radioact. 57, 231–236 (2001).
Fisher, N. S., Guillard, R.R.L., Bankston, D.C. The accumulation of barium by marine phytoplankton grown in culture, J. Mar. Res. 49, 339–354 (1991).
Fisher, N. S. & Wente, M. The release of trace elements by dying marine phytoplankton. Deep Sea Res. Part I 40(4), 671–694 (1993).
Maltsev, Y., Maltseva, K., Kulikovskiy, M. & Maltseva, S. Influence of light conditions on microalgae growth and content of lipids, carotenoids, and fatty acid composition. Biology (Basel) 10(10), 1060 (2021).
Vidmar, T. EFFTRAN—a Monte Carlo efficiency transfer code for gamma-ray spectrometry. Nuclear Instrum. Methods A 550, 603–608 (2005).
Hult, M., & Lutter, G. Detection of 106Ru, via the decay of its daughter 106Rh, in gamma-ray spectra, JRC technical reports JRC108853, Publications Office of the European Union, Luxembourg (2017).
ICRP Age-dependent Doses to Members of the Public from Intake of Radionuclides—Part 1, ICRP Publication 56, Ann. ICRP 20(2) (1989).
Magnusson, B. & Rasmussen, L. Trace metal levels in coastal sea water investigation of Danish waters. Mar. Pollut. Bull. 13(3), 81–84 (1982).
Bruland, K. W., Franks, R. P., Knauer, G. A. & Martin, J. H. Sampling and analytical methods for the determination of copper, cadmium, zinc, and nickel at the nanogram per liter level in sea water. Anal. Chim. Acta 105, 233–245 (1979).
Locatelli, C. Sequential voltammetric determination of ultratrace osmium, ruthenium and iridium. Application to superficial water. Electroanalysis 23, 1329–1336 (2011).
Andreae, M. O. & Froelich, P. N. Arsenic, antimony, and germanium biogeochemistry in the Baltic Sea. Tellus B Chem. Phys. Meteorol. 36(2), 101–117 (1984).
Salbu, B., & Steinnes, E. Trace elements in natural waters, Springer Science & Business, (1994).
Cresswell, L. Phytoplankton Culture for Aquaculture Feed, SRAC Publications No. 5004 (2010).
Slowey, J.F., Hayes, D., Dixon, B., & Hood, D.W. Distribution of gamma-emitting radio-nuclides in the Gulf of Mexico, Proc. Symp. Mar. Geochemistry, Univ. Rhode Island Occas. Publ. 3, pp. 109–29 (1965).
Acknowledgements
This work was funded by the Swedish Radiation Safety Authority under contract number SSM2020-642-24. Additional funding for equipment was received from Wilhelm and Martina Lundgrens foundation, Herbert and Karin Jacobssons foundation and the Royal Academy of Arts and Sciences in Gothenburg. The authors are grateful for useful advice and help from Kristin Andreasson at SMHI, Maria Davidsson at the Ringhals NPP, Olga Kourchenko at the Gothenburg University algal bank GUMACC, Elin Lindehoff at the Linné University and Christian Ekberg at Chalmers University of Technology. Special thanks for invaluable advice on CF determination are directed to Nicholas Fisher at the State University of New York.
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Y.A.: experiment—concentration factors, data analysis; R.T.: methodology, supervision, writing—review and editing; S.H.: experiment—element concentrations, data analysis, writing—review and editing; M.I. methodology, validation, writing—review and editing; K.I.B.: computer simulations, data analysis, methodology, supervision, writing—original draft, funding acquisition.
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Ali, Y., Thomas, R., Holgersson, S. et al. Experimental determination of concentration factors of Ni, Ru and Sb in the model diatom Phaeodactylum tricornutum. Sci Rep 13, 13575 (2023). https://doi.org/10.1038/s41598-023-38795-2
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DOI: https://doi.org/10.1038/s41598-023-38795-2
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