Developmental abnormalities in otoliths can impact growth and survival in teleost fishes. Here, we quantified the frequency and severity of developmental anomalies in otoliths of delta smelt (Hypomesus transpacificus), a critically endangered estuarine fish that is endemic to the San Francisco Estuary. Left–right asymmetry and anomalous crystalline polymorphs (i.e., vaterite) were quantified and compared between wild and cultured populations using digital image analysis. Visual estimates of vaterite were validated using X-ray diffraction, Raman spectroscopy, laser ablation ICPMS, and electron probe microanalysis. Results indicated that cultured delta smelt were 80 times more likely to contain a vateritic otolith and 18 times more likely to contain relatively large (≥ 15%) amounts of vaterite. Similarly, cultured fish exhibited 30% greater asymmetry than wild fish. These results indicate that cultured delta smelt exhibit a significantly higher frequency of vestibular abnormalities which are known to reduce fitness and survival. Such hatchery effects on otolith development could have important implications for captive culture practices and the supplementation of wild fish populations with cultured individuals.
Otoliths, or “ear stones”, are calcified structures in teleost fishes that perform critical sensory functions including hearing, balance, and acceleration1. As biominerals, otoliths are composed primarily of calcium carbonate (CaCO3) embedded with organic molecules (e.g., polysaccharides and proteins), are metabolically inert, and grow continuously through the daily accretion of concentric layers of otolith matrix2,3. The biomineralization of CaCO3 also incorporates a variety of elements from the ambient water, thus providing a record of a fish’s environmental and metabolic history4,5,6. Therefore, otoliths serve as permanent records of the age, growth rate, and life history of fishes, thus providing useful data for informing the management of fisheries and ecosystems2,7.
Three pairs of otoliths (sagittae, lapillae, and asterisci) are present within a fish’s cranium, with each pair being comprised of varying amounts of three different CaCO3 polymorphs: aragonite, vaterite, and calcite8. Within teleost fishes, sagittae and lapilli are often comprised primarily of aragonite whereas asterisci are composed primarily of vaterite, and calcite is occasionally present in smaller amounts2,9. Unlike teleosts, sagittal otoliths of basal groups of fishes (e.g., sturgeons and paddlefishes of the order Acipenseriformes) are composed primarily of vaterite10. Despite these general patterns, the makeup of otoliths can vary considerably within taxonomic groups and even among individuals within a species8. In teleosts, aragonitic sagittae and lapilli are often the largest otoliths and are, therefore, most commonly used in age, growth, and geochemical analyses.
Several developmental abnormalities in otoliths have been described including asymmetry11,12 and crystalline anomalies13,14,15. In particular, the anomalous replacement of aragonite by vaterite in sagittae and lapillae of many teleosts causes these “vateritic” otoliths to become less dense, less stable, and more transparent than usual16,17. Such vateritic otoliths have been observed in many species such as lake trout (Salvelinus namaycush)18, Atlantic herring (Clupea harengus)16, Atlantic salmon (Salmo salar)19, and Chinook salmon (Oncorhynchus tshawytscha)13,20. Vateritic otoliths are most common in hatchery-reared populations of fishes, with up to 50% of otoliths in hatchery fish containing vaterite (versus 8% in wild fish), and with vaterite compositions of up to 91%16,21.
The effects of vestibular abnormalities in fishes remain poorly understood. However, anomalies in otoliths have been associated with acute and chronic stress22,23 and are known to result in reduced function, thus potentially negatively impacting fitness by impairing foraging, navigation, and predator avoidance21,24,25,26,27,28. Moreover, vateritic otoliths often exhibit a loss of otolith microstructures and alteration of relative elemental concentrations, including enrichment in Mn/Ca and Mg/Ca, and depletion in Sr/Ca, Ba/Ca, and Na/Ca; thus complicating age, growth, and life-history reconstructions based on otolith analyses8,18,29,30,31. Therefore, studies quantifying otolith compositions and sources of variation in minerology are needed in order verify interpretations of otolith-based metrics32.
The delta smelt (Hypomesus transpacificus) is an estuarine forage fish that is endemic to the San Francisco Estuary (SFE). This migratory zooplanktivore is adapted to cool, turbid, low-salinity habitats33,34 and exhibits a complex life history that allows it to exist within the dynamic low-salinity habitats of the SFE35. Although historically abundant, its population has declined to < 1% of 1980s levels, leading to its listing as threatened by the federal Endangered Species Act (ESA)36, endangered by the state ESA37, and critically endangered by the International Union for Conservation of Nature38. This population decline is likely due to multiple human impacts including hydrological modifications resulting from dams and exports, direct entrainment in water diversions, pollution, invasive species, trophic collapse, and climate change34. Municipal and agricultural demands for freshwater have made conservation of this imperiled fish a difficult and controversial issue in California politics and resource management39.
To conserve delta smelt, a captive culture program has been established at the Fish Conservation and Culture Laboratory, UC Davis, that produces 20,000–50,000 delta smelt each year to support experiments, serve as a reserve population, and provide cultured fish that can be released to supplement the remaining wild population40,41. However, the effects of hatchery conditions and domestication on the fitness of cultured delta smelt remain poorly understood42. For example, little is known about the comparative health and survival of cultured versus wild individuals, and such differences could influence the outcomes of experiments and supplementation efforts using cultured fish. Similarly, otolith-based analyses have been used to describe environmental effects on growth33 and life history35,43,44 of wild delta smelt; however, the prevalence and potential effects of crystalline anomalies have not been examined. Studies that quantify developmental abnormalities in otoliths, therefore, are important for examining differences in health and fitness among wild and cultured fish populations and for validating the interpretation of otolith-based metrics.
Several approaches can be used to identify variation in the crystalline structure of CaCO3 in otoliths. Visual estimates based on digital image analysis are the most efficient approach; however, such approaches should first be validated using other techniques. Analysis of bulk (mass %) of CaCO3 polymorphs in otoliths can be estimated using X-ray powder diffraction (XRD), whereas spatial distributions in otoliths can be identified using Raman spectroscopy or laser-ablation inductively coupled plasma mass spectroscopy (LA-ICPMS), each being applied across polished otolith surfaces. XRD is a destructive technique that examines the X-ray scattering pattern of a crushed (powdered) crystalline sample to identify the relative abundances of different polymorphs14,45. Raman spectroscopy is a non-destructive technique using excitation by a laser light source and unique spectral shifts to identify spatial patterns in the molecular structure of substrates18,46. LA-ICPMS is a minimally destructive technique that uses a focused laser to ablate a thin surface layer of material from the otolith, with the ablated particles passed to a coupled plasma mass spectrometer to determine their chemical composition. Thus, LA-ICPMS can be used to quantify spatial patterns in the elemental composition of otolith sections, with known chemical variation among different crystalline polymorphs29.
Here, we used an interdisciplinary approach to quantify and contrast vestibular abnormalities, including asymmetry and the presence of irregular CaCO3 crystalline structure (e.g., vaterite) in sagittal otoliths of delta smelt. Specifically, we validated digital image analysis using a combination of XRD, Raman Spectroscopy, and LA-ICPMS geochemistry, and then applied this tool to contrast otolith developmental anomalies between wild and cultured delta smelt populations. We also explored the potential effects of hatchery rearing conditions (e.g., food availability and temperature) on otoliths of the cultured population. By quantifying developmental abnormalities in otoliths of cultured and wild delta smelt populations, we aimed to advance the interpretation of otolith-based metrics and to improve our understanding of the relative health and survival of the cultured population, with important implications for hatchery production and supplementation of the wild population of this critically endangered species.
This study took advantage of archived delta smelt otoliths from previous work conducted as part of the delta smelt Captive Culture Program at the UC Davis Fish Conservation and Culture Laboratory (FCCL). Prior work was carried out in accordance with relevant guidelines and regulations under the UC Davis Institutional Animal Care and Use Committee Protocols #18071 and #19841, with methods reported in accordance with ARRIVE guidelines. In short, cultured (F11) delta smelt were hatched and reared at the FCCL following established protocols41,47. Fish were spawned and reared in freshwater (salinity of 0.4 ppt) at 16 °C for 120 days (~ 50 mm total length), then individually tagged using Visible Implant Alphanumeric (VIA) tags, and randomly distributed among four tanks (88 specimens/tank) with varying temperatures (14 °C or 18 °C) and varying feed (ad libitum or no feed). This resulted in four distinct treatments: 14 °C fed, 14 °C unfed, 18 °C fed, 18 °C unfed. Fish were subsequently monitored for 70 days. After the treatment period, fish were euthanized following approved veterinary procedures using 500 mg/L buffered tricaine methanesulfonate (MS-222) and were then archived in a freezer at − 20 °C.
For the present study, otoliths were removed from archived specimens and stored in 95% ethanol. Upon dissection, significant vaterite could be observed in several otolith samples, thus providing an opportunity to quantify the prevalence of vaterite in cultured delta smelt. Results for cultured fish were compared to those of 104 wild delta smelt that were previously collected in 2019 by the US Fish and Wildlife Service’s Enhanced delta smelt Monitoring (EDSM) Kodiak trawl survey48 (Table 1). Otoliths of wild delta smelt were previously dissected and archived following the same protocols as used for cultured fish. Sex was not included in the analysis because all fish were less than 1 year of age, and delta smelt do not exhibit sexual dimorphism49. The transfer and processing of wild and cultured delta smelt otoliths was approved by the California Department of Fish and Wildlife (CDFW) under a Section 2081a Memorandum of Understanding to L. Lewis (No. 2021-0004-R3_Lewis).
Whole otolith images were taken sulcus side down with an AmScope MU1000 10MP camera on a Leica SteroZoom7 dissecting scope at 20 × or 30 × magnification with a scale bar (1 mm increments). After imaging, otoliths were mounted to glass microscope slides with a thermoplastic glue (Crystalbond) and stored in plastic microscope slide boxes. A subset of otoliths (n = 24) with varying proportions of vaterite were selected to quantify whole otolith polymorph composition using XRD. Otoliths selected for XRD were cleaned with 99.5% acetone to remove any Crystalbond, and were then sonicated in MilliQ water, dried, and stored in clean glass vials prior to analysis. An additional subset of five samples, representing a broad range of vaterite compositions, were selected and prepared for analysis by Raman spectroscopy and laser ablation. These samples were sanded on the sulcus side with 600, 800, and 1200 grit sandpaper and polished using a polishing cloth wetted with 0.3-µm alumina polish. Polished sections were sonicated a second time in MilliQ water prior to analysis.
Digital image analysis
Left and right otoliths from a total of 119 cultured and 104 wild delta smelt were included in digital image analyses (Fig. 1, Table 1), with fish containing a broken or missing otolith being previously excluded (n = 16). Image analysis was used to quantify several otolith metrics using ImageJ (Version 2.0)50,51. Differences in the size of left and right otoliths were used to estimate otolith asymmetry for each fish following Eq. (1), where DVL and DVR are the maximum dorsoventral measurements (in µm) for the left and right otolith, respectively.
The total surface area (Atot, in µm2) of each otolith was measured from a calibrated digital image using the ‘Analyze Particle’ operation in ImageJ52. Aragonitic and vateritic segments of each otolith image were then identified visually based on differences in texture and translucency, with vaterite appearing more transparent. The area of aragonite (Aarag), typically in the center of the otolith, was then measured by drawing a polygon over the aragonitic area using the ‘Freehand’ operation in ImageJ. The surface area of vaterite (Avat) in each otolith was calculated by subtracting Aarag from Atot. Vaterite “prevalence” (percent abundance based on area) was then calculated according to Eq. (2). Vaterite prevalence was quantified for each otolith of each fish, and the most vateritic otolith (‘max’) used to classify each fish into 1 of 4 vaterite categories: I (< 1.0%), II (1–14.9%), III (15–29.9%), IV (> 30.0%) (Fig. 1, Table 1).
Raman spectroscopy was used to validate results from digital image analysis with respect to spatial patterns of vaterite and aragonite in delta smelt otoliths. Raman spectroscopy is a non-destructive technique using excitation by a laser light source and the unique spectral shifts of backscattered light, due to specific bond vibrations, to identify different molecules and their crystalline microstructures18,46. This approach provides discrete spatial observations across 2-dimensional (polished) surfaces. Each measurement yields a Raman spectrum, comprised of numerous peaks, with each peak reflecting a Raman Shift (in wavenumber, cm−1) due to the substrate’s molecular composition. Based on values provided in the literature18,46, we examined multiple peaks that could be used to discriminate between the three CaCO3 polymorphs commonly found in otoliths: aragonite (1085 and 705 cm−1), vaterite (267, 300, 740, 750, 1075, and 1090 cm−1), and calcite (282 and 711 cm−1).
Raman analyses were performed at the Keck Spectral Imaging Facility, UC Davis, using a Renishaw Confocal Raman Microscope equipped with a diode laser with an excitation peak of 785 nm at 0.37 mm working distance, a 50 × objective, and a grating of 1800 mm−1, calibrated to a peak of 520 cm−1 on a silicone standard. Spectra were collected with an integration time of 10 s and a peak range of 130–1200 cm−1. Five otoliths were selected for Raman spectroscopy, spanning the full range of vaterite categories (I–IV). Multiple spots were analyzed along a transect from the otolith core to the ventral edge: (1) aragonite, (2) aragonite edge, (3) vaterite edge, (4) vaterite, with additional radial transects conducted in the most vateritic (category IV) otolith (Fig. 3). Peaks were automatically detected in each spectrum using the “findpeaks” function in the pracma package18,53 in R (v. 3.6.2) and confirmed graphically. The peaks are identified relative to background counts, which are determined by the sample type and machine settings and therefore have arbitrary units. Additional peaks not known to be CaCO3 polymorphs were observed due to contamination by binding resin54 and were thus excluded.
The bulk percent composition of vaterite in otoliths was measured using X-ray diffraction (XRD). XRD is a destructive technique that examines the X-ray scattering pattern of a crushed (powdered) crystalline sample to identify the relative abundances of different polymorphs14,45. A total of 24 previously imaged otoliths from cultured delta smelt, spanning the range of vaterite categories (I–IV), were selected for XRD analysis. Each otolith was then crushed to a fine powder and scanned for 80 min on a PANalytical X’Pert Pro diffractometer equipped with a PW3064 sample spinner and Co X-ray tube X’Celerator detector using a scan range of 20°–70° 2θ with a step size of 0.0017° 2θ. All XRD analyses were performed at the X-Ray Diffraction Lab, UC Berkeley, CA. To validate visual estimates of vaterite, vaterite prevalence based on XRD (bulk %) was contrasted with estimates based on image analysis using linear regression.
Elemental concentrations in otoliths were measured using a Photon Machines 193 nm ArF Excimer laser with a HelEx dual-volume LA cell coupled to a Thermo Element XR HR-ICPMS in the Yin Lab at the Department of Earth and Planetary Sciences at UC Davis. The repetition rate of the laser was set at 10 Hz and fluence was ~ 3 J cm−2. A line of spots was ablated from the edge to the core with a spot size of 40 µm and a spacing of 40 µm. Before data collection, a cleaning run (pre-ablation) was performed across the same trajectory with a larger (80 µm) spot size. We measured a large suite of potentially informative analytes including lithium, sodium, magnesium, potassium, calcium, manganese, zinc, strontium, and barium (7Li, 23Na, 24Mg, 39K, 43Ca, 44Ca, 55Mn, 66Zn, 88Sr, and 138Ba, respectively). Data were reduced with the Trace Element data reduction schema in the Iolite55 software package, with NIST 612 as the standard reference material and 43Ca as the internal standard. Otoliths were assumed to contain 38.8 wt% Ca56. NIST 610 and 612 glasses were measured prior to and between each sample and used as external reference materials to correct for instrument drift. Element-specific limits of detection were calculated as 3 times the standard deviation of the element-specific background value. Measured concentrations of an element below its respective detection limit were set to zero, indicating negligible abundance.
Relative abundances of elements were mapped spatially across the surface of a category IV vateritic otolith using a Cameca SX-100 electron microprobe (Earth and Planetary Sciences, UC Davis) equipped with five WDS spectrometers and a tungsten thermionic emission filament. The WDS spectrometers were tuned to Sr, Ca, Ba, and Mg X-ray lines, with backscatter electron images captured for both compositional and topographical modes. An operating voltage of 15 kV was used, with the beam current regulated at 100 nA with a fully focused beam. The beam was fixed in place and the stage was scanned to capture spatially accurate maps. The maps were acquired as mosaic cells and reconstructed into a single imagen using the Cameca PeakSight 6.5 software. The software was used to adjust the scales of the image for clarity, and color LUTs were applied as well.
Estimates of vaterite composition based on image analysis and XRD were compared by linear regression. Linear models were used to compare vaterite composition and asymmetry among cultured and wild fish. The presence of vaterite was compared between cultured and wild fish by logistic regression. The potential effects of recent hatchery conditions on vaterite and asymmetry in cultured fish were each examined using linear mixed effect models that included temperature, feed, and their interaction as fixed effects and tank as a random effect. Logistic regression was used to assess potential effects of hatchery conditions on vaterite presence. All analyses and plotting were conducted using R statistical analysis software (v. 3.6.2)57.
Comparison of vaterite prevalence based on XRD and image analysis indicated a strong linear positive relationship between the two approaches (p < 0.01, R2 = 0.84, Table 2, Fig. 2). An identification threshold of 10% prevalence was observed (Fig. 2), however, 6 samples were identified as 100% aragonite (0% vaterite) by both methods. This suggests that the observed threshold seen was likely due to the detection limits of XRD for small otolith samples, rather than uncertainty in imaged-based estimates of vaterite prevalence.
Raman spectra of aragonitic otoliths (category I) exhibited peaks indicating 100% aragonite (705, 1085 cm−1), whereas vateritic otoliths (category II–IV) included peaks indicative of both aragonite and vaterite (267, 300, 740, 750, 1075, and 1090 cm−1, Fig. 3b). Peaks at 282 cm−1 were observed in 3 otoliths, possibly indicating some calcite; however, no corresponding calcite peak at 711 cm−1 was observed, while each spectrum also exhibited multiple peaks indicating the presence of either aragonite or vaterite (Fig. 3c). These spots were therefore classified as either aragonite or vaterite, resulting in limited evidence for the presence of calcite in delta smelt otoliths. Peaks at 155 and 206 cm−1 appeared less reliable for discriminating between polymorphs, and were thus excluded from analysis.
We observed significant differences in the elemental compositions of aragonitic and vateritic regions of otoliths. Although Li, K, and Zn were below detection levels, and Ca appeared consistent between crystalline polymorphs, vateritic regions exhibited higher levels of Mg and Mn and lower levels of Na, Ba, and Sr, relative to aragonite. Mg and Mn concentrations were on average 4.6 × and 2.4 × greater in vaterite, while Na, Ba, and Sr were 2x, 14x, and 7.6 × lower in vaterite otoliths, respectively (Fig. 4; Table S1). We did however observe a slight disparity between visual identifications and elemental compositions on otolith aragonite-vaterite edges in Category III and IV otoliths (Fig. S1), likely due to the overlap between polymorphs. Electron probe microanalysis indicated that two-dimensional spatial patterns in the relative abundances of Mg and Sr in otolith sections were consistent with visual patterns in vaterite prevalence and with linear profiles of element-to-calcium ratios based on LA-ICPMS analyses (Fig. 5).
Vaterite and asymmetry in wild and cultured delta smelt
Vaterite presence and prevalence differed significantly between cultured and wild delta smelt. Greater than 68% of cultured fish contained at least one otolith exhibiting some vaterite (> Category I), compared to just 0.8% of wild fish otoliths (p < 0.001, Tables 1 and 3, Fig. 1). Greater than 18% of cultured fish had at least one otolith exhibiting relatively large amounts (> 15%, category III–IV) of vaterite, whereas no wild fish contained category III–IV otoliths. In total, 32% of cultured fish had no vateritic otoliths, 36% with one vateritic otolith, and 32% with both right and left vateritic otoliths. In contrast, 99% wild fish had no vateritic otoliths, < 1% with one vateritic otolith, and 0% with both right and left vateritic otoliths. Similarly, mean (max) prevalence of vaterite in cultured fish was 7.90%, compared to only 0.05% in wild fish (p < 0.001, Table 3, Fig. S2). Otolith asymmetry varied significantly between wild (1.84%) and cultured (2.40%) fish (p = 0.01, Table 3, Fig. S2). Although hatchery conditions may influence otolith development, the recent 70-days rearing conditions for cultured fish had no detectable effects on the presence (p = 0.268) or prevalence (p = 0.393) of vaterite, nor otolith asymmetry (p = 0.519, Table 3, Fig. S2).
Summary of main findings
Here, we quantified the frequency and severity of developmental anomalies in otoliths of delta smelt, a critically endangered estuarine fish that is endemic to the San Francisco Estuary, California, USA. Visual, geochemical, and structural approaches were combined to validate the identification of vaterite in otoliths using digital image analysis. Our study is the first to confirm the presence of vaterite in otoliths of cultured delta smelt, which presented visually as an irregular transparent zone in the otolith and exhibited a distinct signature using LA-ICPMS, XRD, and Raman spectroscopy.
Otoliths of cultured delta smelt were 80 times more likely to contain vaterite (> 1%) and 18 times more likely to contain relatively large (> 15%) amounts of vaterite. Similarly, cultured fish exhibited 1.3 times greater asymmetry than wild fish. Variation in temperature and feed did not appear to affect the presence nor prevalence of vaterite and asymmetry in delta smelt otoliths; however, this may be due in part to the short exposure period of those treatments. In many cases, it was apparent that vaterite formation in otoliths of cultured delta smelt had initiated prior to experimental treatments, suggesting that other culture conditions or longer-term exposures may be responsible for the higher vaterite content in otoliths from the hatchery population. Together, these results indicate that cultured delta smelt exhibit a significantly higher frequency of vestibular abnormalities which can impair hearing, fitness, and survival21,26,28,58. Such hatchery effects on otolith development could have important implications for captive culture practices and the supplementation of the wild delta smelt population with cultured individuals.
Vaterite in otoliths has previously been quantified using visual15,59,60,61,62, structural14, or geochemical analyses30,31. Here, we contrasted results of five different approaches for identifying vaterite in sagittal otoliths of delta smelt. Results based on digital image analysis were highly correlated with those based on XRD, Raman, and geochemical approaches, indicating that visual analysis is a reliable and cost-effective technique for quantifying vaterite in otoliths. For example, 84% of the variation in bulk vaterite content estimated by XRD could be described by visual 2D estimates. Cross referencing spatial patterns in vaterite composition using both Raman spectroscopy and LA-ICPMS with those determined by image analysis further validated the approach. Variation in elemental concentrations as the laser transitioned from aragonite to vaterite regions of the otolith matched expected patterns based on previous studies16,29,63. For example, vateritic regions exhibited higher concentrations of magnesium (Mg) and manganese (Mn), and lower concentrations of sodium (Na), barium (Ba) and strontium (Sr) relative to respective aragonitic regions. Results from Raman spectroscopy largely confirmed visual assessments, but also identified mixed aragonite and vaterite signals across aragonite-vaterite edges, suggesting that transitions may be more gradual than they appear visually (Fig. 3).
Vaterite and asymmetry
The presence of vaterite in cultured (68%) and wild (< 1%) delta smelt otoliths was similar to values observed in cultured and wild populations of other teleost fishes. For example, similar values in cultured populations have been described for Atlantic salmon (66–100%), coho salmon (52–56%), herring (14–60%), rainbow trout (50%), and lake trout (48%)21. As for delta smelt, the presence of vaterite in otoliths of wild fish populations is much lower than that of cultured populations, including coho salmon (1–12%), herring (5–6%), rainbow trout (5%), and lake trout (24%)21. Overall, vaterite presence is on average 10.4 times higher in cultured fish than wild fish, with up to 91% vaterite prevalence in certain species16,21. Otolith asymmetry in wild and cultured delta smelt (1.84–2.40%) was relatively similar to global estimates across numerous taxa (1.25–4.66%)64. Hatchery fish have been shown to exhibit up to threefold greater fluctuating asymmetry than wild fish65, though this trend is not consistent for all species66. Similarly, hatchery delta smelt exhibited 3–4 times higher asymmetry than wild fish, however, even the cultured values remained relatively low.
Causes of vaterite formation
Vaterite is often naturally occurring in fish otoliths, with its presence varying among taxa and otolith types14. For example, sagittal otoliths in sturgeon are largely vateritic, whereas sagittae are aragonitic in most teleost fishes10. Within teleosts, sagittae and lapilli are typically comprised of aragonite, whereas asterisci are commonly vateritic2,9. The biomineralization of otoliths is tightly regulated through complex patterns in gene expression within the vestibular apparatus and associated variation in protein synthesis and enzymatic activity9,67,68,69,70,71. For example, otolith formation in zebrafish is halted when the expression of the otop1 gene is inhibited70. Thus, natural variation in otolith crystalline structures among species, and among otolith types within species, are likely linked to variation in genes and gene expression among taxa and regions of the inner ear of fishes.
Here, we demonstrate that sagittal otoliths of delta smelt can switch from aragonite (normal) to vaterite (abnormal), and that this phenomenon is far more common in cultured versus wild individuals. While the causes of anomalous vaterite formation in otoliths are not fully understood, otolith development appears to be sensitive to environmental conditions and fish bioenergetics. For example, anomalously high levels of vaterite in teleost sagittae have been associated with rapid growth19, elevated temperatures72, high stocking densities28, and handling stress60. Similarly, variation in water chemistry may affect otolith biomineralization, with elevated pCO2 and reduced pH of ambient water associated with increases in an anomalous (calcite) CaCO3 polymorph73,74. Changes in [Ca2+]/[CO32−] within the endolymph is one hypothesized mechanism for stress-induced changes in otolith biomineralization75,76. Stress-induced changes in the expression of different otolith macromolecules (e.g., OMM-64 and Otolin-1) may also result in changes in the crystalline structures of otoliths54,69,77,78.
Although our study was not designed to determine the causes of abnormal otolith development, we nevertheless examined evidence for the effects of temperature, food availability, and their interaction on otolith asymmetry and vaterite formation (Table 3). Here, we detected no significant treatment effects on vaterite or asymmetry in delta smelt otoliths (Table 3). The present study, however, may not fully capture the true nature of environmental and trophic effects on vaterite formation. For example, the short (70-days) duration and limited thermal range of the experimental treatments imposed on cultured fish may have not been sufficient to elicit a measurable effect. Furthermore, the presence of vaterite in some cultured fish prior to their placement in experimental treatments suggests that longer exposures during earlier life stages may result in stronger treatment effects. Targeted experiments that impose longer treatments and wider ranges of culture conditions on younger age-classes may further advance our understanding of the drivers of otolith anomalies in teleosts.
Consequences of otolith abnormalities
The effects of otolith anomalies on fitness could have important implications for fisheries management and conservation7,19,28. For example, the development of vateritic otoliths does not appear to be reversible21 and is correlated with several other developmental abnormalities including fewer lateral line neuromasts (i.e., sensory epithelial hair cells), smaller brain weight, and reduced olfactory bulb volume61. In adult Norwegian Atlantic salmon, for example, higher frequencies of vaterite corresponded with lower return rates to freshwater rearing habitats28, possibly due to reduced sensory function, behavioral alteration, and navigational impairment associated with abnormal otolith development21,26,27. As for otolith polymorph anomalies, otolith asymmetry can also impair hearing25, cause abnormal swimming behaviors58, and limit a fish’s ability to disperse, navigate, and recruit to suitable habitats79.
A key concern regarding supplementation of wild populations with cultured fish is whether domesticated populations will exhibit similar fitness and survival as wild populations80. The higher prevalence of vaterite observed in cultured delta smelt, for example, may correspond with reduced survival in situ, thus impacting the success of supplementation28. For example, altered swimming behavior in delta smelt due to temperature stress leads to higher susceptibility to non-native predators81. Furthermore, vestibular and behavioral impairment due to otolith abnormalities may be more common or severe in smaller-bodied fishes such as delta smelt21,24,27. The effects of vaterite development on the survival of fish in the wild remains unclear, and effects likely vary ontogenetically82. Quantification of the realized effects of vestibular abnormalities in situ remains an important frontier for fisheries management and conservation7,28,82.
In addition to physiological effects on fishes, variation in otolith minerology can alter the interpretation of otolith-based metrics8,32. This is because polymorph-dependent changes in otolith chemistry can mimic changes associated with environmental variation, such as seasonality or migrations. For example, variation in the minerology of European eel (Anguilla anguilla) otoliths resulted in the misidentification of their migratory history29. Vaterite in delta smelt sagittae exhibited higher concentrations of Mg and Mn and lower concentrations of Na, Ba, and Sr relative to aragonite, thus proving to be consistent with prior studies, and confirming the importance of otolith minerology for the accurate interpretation of geochemical patterns in otoliths16,29,63.
Although vaterite was uncommon in wild delta smelt, the significant quantities of vaterite observed in cultured delta smelt could distort the interpretation of patterns in accretion and geochemistry of hatchery-reared delta smelt. This is particularly concerning given that supplementation of the wild population remains a key conservation strategy. However, most of the vaterite observed in cultured delta smelt was present only on rostral, ventral, and postrostral lobes of the otolith, with the dorsal lobe remaining mostly free of any vaterite. Thus, results from studies that utilize the dorsal lobe of delta smelt otoliths are likely less susceptible to variation in otolith minerology33,35,44,51. The quantification of such patterns is key to developing robust methods in otolith research8,32.
Here we quantified the prevalence of otolith crystalline anomalies and asymmetry in wild and cultured populations of delta smelt, a critically endangered estuarine fish that is currently supported by a conservation hatchery. By integrating multiple analytical techniques, we demonstrated that digital image analysis is an effective tool for the rapid quantification of vaterite in fish otoliths. Both vaterite and asymmetry were elevated in otoliths of cultured fish relative to those from the wild population. Although the causes of these anomalies are not fully understood, our results suggests that either (a) hatchery conditions increase otolith abnormalities in cultured fish or (b) that such abnormalities negatively affect fitness and survival, thus leading to strong selection in the wild. Further studies are needed to identify causal mechanisms of vaterite formation in delta smelt and how such abnormalities might affect the hatchery population and the effectiveness of population supplementation efforts.
All data generated and analyzed during this study are available from the corresponding author upon request.
Popper, A. N., Ramcharitar, J. & Campana, S. E. Why otoliths? Insights from inner ear physiology and fisheries biology. Mar. Freshw. Res. 56, 497 (2005).
Campana, S. E. Chemistry and composition of fish otoliths: Pathways, mechanisms and applications. Mar. Ecol. Prog. Ser. 188, 263–297 (1999).
Thomas, O. R., Richards, K. L., Petrou, S., Roberts, B. R. & Swearer, S. E. In situ 3D visualization of biomineralization matrix proteins. J. Struct. Biol. 209, 107448 (2020).
Izzo, C., Reis-Santos, P. & Gillanders, B. M. Otolith chemistry does not just reflect environmental conditions: A meta-analytic evaluation. Fish Fish. 19, 441–454 (2018).
Elsdon, T. S. et al. Otolith chemistry to describe movements and life -history parameters of fishes: Hypotheses, assumptions, limitations and inferences. in Oceanography and Marine Biology (CRC Press, 2008).
Walther, B. D. The art of otolith chemistry: Interpreting patterns by integrating perspectives. Mar. Freshw. Res. 70, 1643 (2019).
Reis-Santos, P. et al. Reading the biomineralized book of life: Expanding otolith biogeochemical research and applications for fisheries and ecosystem-based management. Rev. Fish Biol. Fish. (2022).
Pracheil, B. M., George, R. & Chakoumakos, B. C. Significance of otolith calcium carbonate crystal structure diversity to microchemistry studies. Rev. Fish Biol. Fish. 29, 569–588 (2019).
Thomas, O. R. B. & Swearer, S. E. Otolith biochemistry—A review. Rev. Fish. Sci. Aquac. 27, 458–489 (2019).
Pracheil, B. M. et al. Sturgeon and paddlefish (Acipenseridae) sagittal otoliths are composed of the calcium carbonate polymorphs vaterite and calcite. J. Fish Biol. 90, 549–558 (2017).
Panfili, J., Durand, J.-D., Diop, K., Gourène, B. & Simier, M. Fluctuating asymmetry in fish otoliths and heterozygosity in stressful estuarine environments (West Africa). Mar. Freshw. Res. 56, 505–516 (2005).
Díaz-Gil, C. et al. Otolith fluctuating asymmetry: A misconception of its biological relevance?. ICES J. Mar. Sci. 72, 2079–2089 (2015).
Gauldie, R. W. Vaterite otoliths from Chinook salmon (Oncorhynchus tshawytscha). N. Z. J. Mar. Freshw. Res. 20, 209–217 (1986).
Gauldie, R. W. Polymorphic crystalline structure of fish otoliths. J. Morphol. 218, 1–28 (1993).
Sweeting, R. M., Beamish, R. J. & Neville, C. M. Crystalline otoliths in teleosts: Comparisons between hatchery and wild coho salmon (Oncorhynchus kisutch) in the Strait of Georgia. Rev. Fish Biol. Fish. 14, 361–369 (2004).
Tomás, J. & Geffen, A. J. Morphometry and composition of aragonite and vaterite otoliths of deformed laboratory reared juvenile herring from two populations. J. Fish Biol. 63, 1383–1401 (2003).
Kamhi, S. R. On the structure of vaterite CaCO3. Acta Crystallogr. 16, 770–772 (1963).
Melancon, S., Fryer, B. J., Ludsin, S. A., Gagnon, J. E. & Yang, Z. Effects of crystal structure on the uptake of metals by lake trout (Salvelinus namaycush) otoliths. Can. J. Fish. Aquat. Sci. 62, 2609–2619 (2005).
Reimer, T. et al. Rapid growth causes abnormal vaterite formation in farmed fish otoliths. J. Exp. Biol. 220, 2965–2969 (2017).
Zhang, Z., Beamish, R. J. & Riddell, B. E. Differences in otolith microstructure between hatchery-reared and wild Chinook salmon (Oncorhynchus tshawytscha). Can. J. Fish. Aquat. Sci. 52, 344–352 (1995).
Reimer, T., Dempster, T., Warren-Myers, F., Jensen, A. J. & Swearer, S. E. High prevalence of vaterite in sagittal otoliths causes hearing impairment in farmed fish. Sci. Rep. 6, 25249 (2016).
Campana, S. E. Otolith science entering the 21st century. Mar. Freshw. Res. 56, 485 (2005).
Kern, Z. et al. Fusiform vateritic inclusions observed in European eel (Anguilla anguilla L.) sagittae. Acta Biol. Hung. 68, 267–278 (2017).
Kondrachuk, A. V. Mass and mechanical sensitivity of otoliths. Adv. Space Res. 32, 1521–1526 (2003).
Lychakov, D. V. & Rebane, Y. T. Fish otolith mass asymmetry: Morphometry and influence on acoustic functionality. Hear. Res. 201, 55–69 (2005).
Oxman, D. S. et al. The effect of vaterite deposition on sound reception, otolith morphology, and inner ear sensory epithelia in hatchery-reared Chinook salmon (Oncorhynchus tshawytscha). Can. J. Fish. Aquat. Sci. 64, 1469–1478 (2007).
Vignon, M. & Aymes, J.-C. Functional effect of vaterite—The presence of an alternative crystalline structure in otoliths alters escape kinematics of the brown trout. J. Exp. Biol. 223, 222034 (2020).
Austad, B., Vøllestad, L. A. & Foldvik, A. Frequency of vateritic otoliths and potential consequences for marine survival in hatchery-reared Atlantic salmon. J. Fish Biol. 98, 1401–1409 (2021).
Tzeng, W. et al. Misidentification of the migratory history of anguillid eels by Sr/Ca ratios of vaterite otoliths. Mar. Ecol. Prog. Ser. 348, 285–295 (2007).
Veinott, G. I., Porter, T. R. & Nasdala, L. Using Mg as a proxy for crystal structure and Sr as an indicator of marine growth in vaterite and aragonite otoliths of aquaculture rainbow trout. Trans. Am. Fish. Soc. 138, 1157–1165 (2009).
Budnik, R. R., Farver, J. R., Gagnon, J. E. & Miner, J. G. Trash or treasure? Use of sagittal otoliths partially composed of vaterite for hatchery stock discrimination in steelhead. Can. J. Fish. Aquat. Sci. 77, 276–284 (2020).
Wood, R. S. et al. Quantifying fish otolith mineralogy for trace-element chemistry studies. Sci. Rep. 12, 2727 (2022).
Lewis, L. S. et al. Otolith-based approaches indicate strong effects of environmental variation on growth of a critically endangered estuarine fish. Mar. Ecol. Prog. Ser. 676, 37–56 (2021).
Moyle, P. B., Brown, L. R., Durand, J. R. & Hobbs, J. A. Delta smelt: Life history and decline of a once-abundant species in the San Francisco Estuary. San Franc. Estuary Watershed Sci. 14 (2016).
Hobbs, J. A., Lewis, L. S., Willmes, M., Denney, C. & Bush, E. Complex life histories discovered in a critically endangered fish. Sci. Rep. 9, 16772 (2019).
US Fish and Wildlife. Endangered and threatened wildlife and plants: Determination of threatened status for the delta smelt. US Dep. Inter. Fish Wildl. Serv. 58 (1993).
California Department of Fish and Wildlife. State and Federally Listed Endangered and Threatened Animals of California 31 (2021).
NatureServe. Hypomesus transpacificus: NatureServe: The IUCN Red List of Threatened Species 2014: e.T10722A174778740. (2014).
Moyle, P. B., Hobbs, J. A. & Durand, J. R. Delta smelt and water politics in California. Fisheries 43, 42–50 (2018).
Lessard, J. et al. Considerations for the use of captive-reared delta smelt for species recovery and research. San Franc. Estuary Watershed Sci. 16 (2018).
Lindberg, J. C. et al. Aquaculture methods for a genetically managed population of endangered delta smelt. North Am. J. Aquac. 75, 186–196 (2013).
Hobbs, J. A., Moyle, P. B., Fangue, N. A. & Connon, R. E. Is extinction inevitable for delta smelt and longfin smelt? An opinion and recommendations for recovery. San Franc. Estuary Watershed Sci. 15 (2017).
Hobbs, J. A., Bennett, W. A., Burton, J. & Gras, M. Classification of larval and adult delta smelt to nursery areas by use of trace elemental fingerprinting. Trans. Am. Fish. Soc. 136, 518–527 (2007).
Willmes, M. et al. Calibrating temperature reconstructions from fish otolith oxygen isotope analysis for California’s critically endangered Delta Smelt. Rapid Commun. Mass Spectrom. 33, 1207–1220 (2019).
Carlström, D. A crystallographic study of vertebrate otoliths. Biol. Bull. 125, 441–463 (1963).
Nehrke, G., Poigner, H., Wilhelms-Dick, D., Brey, T. & Abele, D. Coexistence of three calcium carbonate polymorphs in the shell of the Antarctic clam Laternula elliptica. Geochem. Geophys. Geosystems 13, Q05014 (2012).
Hammock, B. G. et al. The health and condition responses of Delta Smelt to fasting: A time series experiment. PLoS One 15, e0239358 (2020).
USFWS et al. Interagency Ecological Program and US Fish and Wildlife Service: San Francisco Estuary Enhanced Delta Smelt Monitoring Program data, 2016–2020 (2020).
Wang, J. Spawning, early life stages, and early life histories of the osmerids found in the Sacramento-San Joaquin Delta of California. US Bur. Reclam. 38, 110 (2007).
Abràmoff, M. D., Magalhães, P. J. & Ram, S. J. Image Processing with ImageJ. 11, 36–42 (2004).
Xieu, W. et al. Experimental validation of otolith-based age and growth reconstructions across multiple life stages of a critically endangered estuarine fish. PeerJ 9, e12280 (2021).
Igathinathane, C., Pordesimo, L. O., Columbus, E. P., Batchelor, W. D. & Methuku, S. R. Shape identification and particles size distribution from basic shape parameters using ImageJ. Comput. Electron. Agric. 63, 168–182 (2008).
Gauldie, R. W., Sharma, S. K. & Volk, E. Micro*Raman spectral study of vaterite and aragonite otoliths of the Coho Salmon, Oncorhynchus kisutch. Comp. Biochem. Physiol. A Physiol. 118, 753–757 (1997).
Jolivet, A., Fablet, R., Bardeau, J.-F. & de Pontual, H. Preparation techniques alter the mineral and organic fractions of fish otoliths: Insights using Raman micro-spectrometry. Anal. Bioanal. Chem. 405, 4787–4798 (2013).
Paton, C., Hellstrom, J., Paul, B., Woodhead, J. & Hergt, J. Iolite: Freeware for the visualisation and processing of mass spectrometric data. J. Anal. At. Spectrom. 26, 2508–2518 (2011).
Hüssy, K., Gröger, J., Heidemann, F., Hinrichsen, H.-H. & Marohn, L. Slave to the rhythm: Seasonal signals in otolith microchemistry reveal age of eastern Baltic cod (Gadus morhua). ICES J. Mar. Sci. 73, 1019–1032 (2016).
R Core Team. R: A language and environment for statistical computing (2019).
Anken, R., Knie, M. & Hilbig, R. Inner ear otolith asymmetry in late-larval cichlid fish (Oreochromis mossambicus, Perciformes) showing kinetotic behaviour under diminished gravity. Sci. Rep. 7, 15630 (2017).
Strong, M. B., Neilson, J. D. & Hunt, J. J. Aberrant crystallization of pollock (Pollachius virens) otoliths. Can. J. Fish. Aquat. Sci. 43, 1457–1463 (1986).
Bowen, C. A. II., Bronte, C. R., Argyle, R. L., Adams, J. V. & Johnson, J. E. Vateritic sagitta in wild and stocked lake trout: Applicability to stock origin. Trans. Am. Fish. Soc. 128, 929–938 (1999).
Brown, A. D., Sisneros, J. A., Jurasin, T., Nguyen, C. & Coffin, A. B. Differences in lateral line morphology between hatchery- and wild-origin steelhead. PLoS One 8, e59162 (2013).
Mäkinen, K., Rajasilta, M., Mäkilä, E., Jokinen, S. & Hänninen, J. Varying frequency of vateritic otoliths in the Baltic herring Clupea harengus membras. J. Fish Biol. 101, 741–744 (2022).
Hüssy, K. et al. Trace element patterns in otoliths: The role of biomineralization. Rev. Fish. Sci. Aquac. 29(4), 445–477 (2021).
Mahé, K. et al. Directional bilateral asymmetry in otolith morphology may affect fish stock discrimination based on otolith shape analysis. ICES J. Mar. Sci. 76, 232–243 (2019).
Koeberle, A. L., Arismendi, I., Crittenden, W., Leer, D. & Noakes, D. L. G. Fluctuating asymmetry of adult Chinook Salmon (Oncorhynchus tshawytscha) otoliths from wild and hatchery origins. Aquat. Ecol. 54, 431–446 (2020).
Geladakis, G., Somarakis, S. & Koumoundouros, G. Differences in otolith shape and fluctuating-asymmetry between reared and wild gilthead seabream (Sparus aurata Linnaeus, 1758). J. Fish Biol. 98, 277–286 (2021).
Falini, G., Fermani, S., Vanzo, S., Miletic, M. & Zaffino, G. Influence on the formation of aragonite or vaterite by otolith macromolecules. Eur. J. Inorg. Chem. 2005, 162–167 (2005).
Ren, D., Feng, Q. & Bourrat, X. The co-effect of organic matrix from carp otolith and microenvironment on calcium carbonate mineralization. Mater. Sci. Eng. C 33, 3440–3449 (2013).
Thomas, O. R. et al. The inner ear proteome of fish. FEBS J. 286, 66–81 (2019).
Hughes, I. et al. Otopetrin 1 is required for otolith formation in the zebrafish Danio rerio. Dev. Biol. 276, 391–402 (2004).
Söllner, C. et al. Control of crystal size and lattice formation by starmaker in otolith biomineralization. Science 302(5643), 282–286 (2003).
Gauldie, R. W. Effects of temperature and vaterite replacement on the chemistry of metal ions in the otoliths of Oncorhynchus tshawytscha. Can. J. Fish. Aquat. Sci. 53, 2015–2026 (1996).
Coll-Lladó, C., Giebichenstein, J., Webb, P. B., Bridges, C. R. & Garcia de la Serrana, D. Ocean acidification promotes otolith growth and calcite deposition in gilthead sea bream (Sparus aurata) larvae. Sci. Rep. 8, 8384 (2018).
Coll-Lladó, C. et al. Pilot study to investigate the effect of long-term exposure to high pCO2 on adult cod (Gadus morhua) otolith morphology and calcium carbonate deposition. Fish Physiol. Biochem. 47, 1879–1891 (2021).
Grosell, M. 4—CO2 and calcification processes in fish. In Fish Physiology Vol. 37 (eds Grosell, M. et al.) 133–159 (Academic Press, 2019).
Payan, P. et al. Effects of stress on plasma homeostasis, endolymph chemistry, and check formation during otolith growth in rainbow trout. Can. J. Fish. Aquat. Sci. 61, 9 (2004).
Tohse, H., Saruwatari, K., Kogure, T., Nagasawa, H. & Takagi, Y. Control of polymorphism and morphology of calcium carbonate crystals by a matrix protein aggregate in fish otoliths. Cryst. Growth Des. 9, 4897–4901 (2009).
Poznar, M. et al. Fish otolith matrix macromolecule-64 (OMM-64) and its role in calcium carbonate biomineralization. Cryst. Growth Des. 20, 5808–5819 (2020).
Gagliano, M., Depczynski, M., Simpson, S. D. & Moore, J. A. Y. Dispersal without errors: Symmetrical ears tune into the right frequency for survival. Proc. R. Soc. B Biol. Sci. 275, 527–534 (2008).
Hung, T. et al. A pilot study of the performance of captive-reared delta smelt Hypomesus transpacificus in a semi-natural environment. J. Fish Biol. 95, 1517–1522 (2019).
Davis, B. E. et al. Consequences of temperature and temperature variability on swimming activity, group structure, and predation of endangered delta smelt. Freshw. Biol. 64, 2156–2175 (2019).
Delaval, A., Solås, M. R., Skoglund, H. & Salvanes, A. G. V. Does vaterite otolith deformation affect post-release survival and predation susceptibility of hatchery-reared juvenile Atlantic salmon?. Front. Vet. Sci. 8, 1066 (2021).
The authors thank the many members of the Fish Conservation and Culture Laboratory (FCCL) and Otolith Geochemistry and Fish Ecology Laboratory at UC Davis (OGFL) who contributed to this study. We are also grateful for comments provided by K. Arend which greatly improved the manuscript. Cody Chalker assisted with collection of Raman spectroscopy data at the Keck Spectral Imaging Facility. Wild specimens were generously provided by the EDSM Survey of the US Fish and Wildlife Service. Delta smelt otoliths were maintained at the OGFL in accordance with a California Department of Fish and Wildlife Service Section 2081a Memorandum of Understanding to L. Lewis. Funding for this study was provided by the US Bureau of Reclamation (USBR) contracts R13AP20022 and R17AC00129 to J. Hobbs, S. Teh, and L. Lewis, and contract R15AC00030 to T.C. Hung. Additional support was provided by the Delta Stewardship Council (DSC) via postdoctoral fellowships to M. Willmes (Grant No. 1167) and L. Lewis (Grant Nos. 2279, 5298). The content of this material and views described herein do not necessarily reflect the views and policies of the USBR, DSC, or UC Davis; nor does mention of trade names or commercial products constitute endorsement or recommendation for use.
The authors declare no competing interests.
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Lewis, L.S., Huang, J.L., Willmes, M. et al. Visual, spectral, and microchemical quantification of crystalline anomalies in otoliths of wild and cultured delta smelt. Sci Rep 12, 20751 (2022). https://doi.org/10.1038/s41598-022-22813-w