The recent need for remote health wellness monitoring has led to the extensive use of wearable sensors. Owing to their increased use, these sensors are required to exhibit both functionality and safety to the user. A major component in the fabrication of these sensors and their associated circuitry is the use of metallic/organic conductive inks. However, very less is known about the interfacial and molecular interactions of these inks with biological matter as they can result in an inflammatory reaction to the user. Significant efforts are thus needed to explore and improve the bio-acceptability of such conductive ink-based wearable sensors. The present study investigates the biocompatibility of encapsulated and non-encapsulated wearable electrochemical sensors used for sensing uric acid as a biomarker for wound healing fabricated using screen-printing technique. Ionic release of metallic ions was investigated first to understand the susceptibility of the conductive inks towards ionic leaching when in contact with a fluid. Time-lapse investigation using ICPS (inductive couple plasma spectroscopy) shows a high concentration (607.31 ppb) of leached silver (Ag+) ions from the non-encapsulated sensors. The cell viability data suggests a 2.5-fold improvement in the sensor biocompatibility for an encapsulated sensor. While the carbon ink shows negligible effect on cell viability, the silver ink elicits significant decrease (< 50%) in cell viability at concentrations higher than 2 mg ml-1. The toxicity pathway of these sensors was further determined to be through the generation of reactive oxygen species resulting in over 20% apoptotic cell death. Our results show that the lower biocompatibility of the non-encapsulated sensor attributes to the higher leaching of Ag+ ions from the printed inks which elicits several different inflammatory pathways. This work highlights the importance biocompatibility evaluation of the material used in sensor fabrication to develop safe and sustainable sensors for long-term applications.
The recent escalation in the role of wearable devices for health and disease management has led to their usage for extended periods. Given the extensive usage, recent reports show that these devices can elicit inflammatory reactions and topical irritations1. A commonly reported side effect of continued use of wearable activity trackers is shown to be a skin rash2. Several research groups around the globe are working on identifying the potential causes for these reactions.Some studies attributed the induced inflammation to nickel leaching. The casings of most of these wearable devices are made of an alloy of stainless-steel containing nickel. The studies show that nickel can cause allergic contact dermatitis3,4, which can further spread to other parts of the body. Another study reported that the cause of localized contact dermatitis is the leaching of acrylate from the rechargeable battery housing3. The literature suggests that the management of inflammatory skin conditions triggered by wearable devices can be challenging. The present study thus attempts to understand the source of these inflammatory responses for the future development of biocompatible and sustainable devices.
Conductive inks have been extensively investigated for the development of wearable devices, such as physical or electrochemical sensors5,6, and flexible electronics7,8,9. Screen-printing of these conductive inks has been of particular interest owing to the techniques’ ease of fabrication and low cost. In contrast to other traditional fabrication routes that use conductive inks such as nanoimprint lithography10,11, and inkjet printing12,13,14 screen-printing allows for rapid fabrication and easy design customization, thus attracting a lot of attention15,16. Typically, these printed sensors interface directly with the user’s skin to acquire the required readings. These conductive inks are generally a heterogeneous mix of several constituent materials, some of which can induce an undesirable biological response if leached into the user’s body. Given that they are placed in direct contact with the skin, the user will be exposed to these materials primarily through the transdermal route. To further add to the problem, depending on the structural properties, certain materials have also been observed to cross the epithelial barrier of the skin and enter the blood circulation17,18,19. The translocation of these materials to secondary organs may result in widely distributed toxic effects. We thus hypothesize that material leaching through these conductive inks may be a potential cause for the reported inflammatory responses.
Two primarily used conductive materials for the development of such inks are carbon and silver. Several allotropes of carbon have been used for the synthesis of conductive inks20,21. Recent studies conducted to understand the potential toxic and immunological effects of carbon-based materials showed that their toxicity is dependent on several factors, such as their size and structure22. However, given the inherent biocompatible nature of the carbon-based materials used in these inks, the biocompatibility of carbon inks has been of little or no concern. On the other hand, owing to their high electrical and thermal conductivity, silver inks have widespread applications in the fabrication of wearable sensors and conductive traces in flexible electronics23,24. The biocompatibility of silver, especially in nanoparticle form, has always remained a concern, significantly limiting its practical usage. Silver shows toxicity towards a wide array of biological systems including mammalian cells, bacteria, and fungal cells27. While several studies25,26,27,28 have been conducted to understand its toxicity in various forms, the toxicity of silver leached through printed conductive inks is yet to be assessed. Silver nanoparticles are known to leach out from several products that contain silver when placed in contact with a fluid29,30,31. Our study hypothesizes that printed conductive silver inks will leach out silver ions when exposed to a (bio)fluid. Previous studies have shown that physicochemical properties of silver particles, e.g., particle size or surface charge, influence their toxic behavior. Several different studies have unanimously reported that smaller silver particles exhibit higher toxicity when compared to their larger counterparts25,26. Moreover, a study carried out by Smith et al., showed that silver ions elicit higher toxicity when compared to silver nanoparticles due to their smaller size and surface charge32. Also, the presence of other ions/metal complexes in the environment has been known to affect the toxicity profile33,34. One such study showed that the toxic response of silver ions can be mediated by the presence of chloride ions in the medium35. This study is especially interesting in the context of wearable electrochemical sensors since inks containing silver in combination with silver chloride are commonly used for the fabrication of reference electrodes. In addition to considering the effect of the leached particles’ properties and their environment, the overall packaging of the wearable device should also be accounted for. Seamless interfacing of wearable devices with the complex contours of the tissue requires high flexibility. Polymers are well suited for this purpose and have thus been widely used to encapsulate such wearable devices36,37,38. However certain polymers, for instance, isobornyl acrylate, can act as allergens, yet again resulting in an inflammatory skin response39,40. Assessment of the toxic response of polymer-encapsulated conductive inks is a knowledge gap that is yet to be addressed.
In this work, we explore the biocompatibility of two commercially available conductive inks, namely, carbon, and silver/silver chloride. As a proof of concept, a standard three-electrode electrochemical sensor, screen-printed on a flexible substrate using conductive inks, was selected for this study. The bio-acceptability assessment of the printed sensors was performed through in vitro cell assays. Since keratinocytes primarily constitute the human epidermis, it was used as the surrogate cell line in this study to investigate its response to the printed sensors. The study first aims to understand the effect of encapsulation on material leaching from the two printed inks through a mass spectrometric analysis. Following this, the toxicity profile of the differently encapsulated printed inks was investigated at cellular and intracellular level on an epidermal (HaCaT) cell line to identify the toxicity pathway of the leached particles. The interaction of silver and carbon ink with cells and their concentration-dependent changes in the cellular function, generation of reactive oxygen species, and apoptotic activity of the cells are detailed in this work (Fig. 1). A comprehensive understanding of the toxicity pathway of printed inks developed through this study can be used to inform a sustainable and biocompatible design pathway for future wearable devices.
Assessment of ion leaching
In our case the wearable device monitors uric acid levels as function of wound healing, where the sensors are used for extended periods (> 5 days). In such prolong use the screen-printed conductive inks may leach out toxic ions, potentially leading to adverse skin inflammation. Quantification of the ion dissolution and minimization of this leaching is therefore crucial. To analyze the leaching of carbon and Ag+ ions from the printed inks, the non-encapsulated and encapsulated sensors were incubated in de-ionized water for varying periods (up to 7 days) and quantified using inductively coupled plasma mass spectrometry (ICP-MS). Only trace levels (≤ 11 ppb) of carbon were detected for both the non-encapsulated and encapsulated sensors, indicating that negligible dissolution of carbon occurs (Fig. 2b). Alternatively, for both cases, a linear increase in Ag+ ion concentration was observed with time. After a 24 h incubation period, the concentration was 210 ppb and 100 ppb for the non-encapsulated and encapsulated sensor, respectively. The concentration of leached ions from the encapsulated sensors was seen to be ~ 2–3 times lower compared to the non-encapsulated sensors (Fig. 2a). While the extract incubated with a non-encapsulated sensor showed an Ag+ ion concentration of 607.31 ppb after a 7-day incubation period, the encapsulated sensor exhibited only a 309.19 ppb concentration. The mass spectrometric analysis confirmed that Ag+ ions leach out of printed inks and the polymeric encapsulation effectively inhibits the leaching.
Exploring Ionic leaching from conductive inks and its correlation to cytotoxicity
Assessing the cytotoxicity of particles or ions leaching from the inks in sensors placed in direct contact with the body is vital. The effect of silver ink on cell viability was expressed as a dose–response curve as shown in Fig. 3. A 24 h exposure to the non-encapsulated silver ink in the 0.125–2.0 mg ml-1 range resulted in a steep decrease in the cell viability (< 60% at higher concentrations), with the estimated 70% inhibitory concentration (IC70) being 0.25 ± 0.02 mg ml-1.
On the other hand, the encapsulated sensor showed only a marginal change. Cell viability after a 24 h exposure to inks at concentrations ≥ 0.5 mg ml-1 was > 90%, but exposure to higher concentrations exhibited viability in the 65–75% range. The higher cell viability in the case of the encapsulated sensor can be attributed to the hindered ionic (Ag+) leaching through the polymeric encapsulation layer. The results imply that encapsulation results in 22% reduced ionic leaching in turn leading to lower toxicity levels. Based on the above results, the IC70 concentration of the silver ink and the highest concentration of carbon ink was selected to further determine their toxicity pathway. To give a better understanding of sensor biocompatibility we further, evaluated the toxicity assessment of the encapsulated sensor in terms of ROS generation and caspase activation.The MTT assay showed that while carbon ink exhibited activity comparable to that of the control cells under all concentrations, the silver ink was cytotoxic (Fig. 3). Since the non-encapsulated carbon ink even at maximum concentrations (2 mg ml-1) caused a non-significant decrease in the cell viability (> 80%), carbon ink was excluded from further investigations to determine the toxicity pathway.
Assessment of reactive oxygen species
Reactive oxygen species are generated as by-products during mitochondrial electron transport or as intermediates of metal-catalyzed oxidation reactions. The sequential reduction of oxygen leads to the formation of ROS, which includes superoxide, hydrogen peroxide, hydroxyl ion, hydroxyl radical, and nitric oxide. An imbalance of ROS in the cells is termed oxidative stress. It can be seen from Fig. 4 that ROS generation is triggered when the epidermal cells are exposed to the encapsulated sensors. This is indicated by the red fluorescent cells shown in the images. Post 24 h we also observe that cells start to deform and detach suggesting ROS trigged cellular death. The results were indicative of the fact that although encapsulation prevents Ag+ ion leaching, it does not prevent inflammation. Therefore, more suitable encapsulation strategies or materials are needed for the development of future wearable sensors. To further investigate the pathway and evaluate the mode of cell death, we carried out a caspase assay.
Assessment of cellular apoptosis
Caspase-3 and caspase-7, also known as executioner caspases, are major and early indicators of apoptosis. These caspases are activated following the leakage of cytochrome C from the mitochondria. Apoptotic cells were observed with Caspase 3–7 staining, with apoptotic cells stained as a bright green nucleus. The cells with a green nucleus in the merged images in Fig. 5 represent the apoptotic cells when subjected to the encapsulated sensors for 24 h. Post 24 h exposure the cells showed a positive caspase activity. As depicted in the figure, about 20% of the cells were apoptotic when subjected to sensors, indicating a high apoptosis-inducing ability. Our previous results had shown a high release of Ag+ ions from the sensors. The apoptotic results confirm that the Ag+ ions leached from the encapsulated inks induce cell suicide via the intrinsic pathway. A higher concentration of leached ions thus leads to a higher apoptotic activity. Cellular death is a slow process and the effect of sensor inducing cell death can only be observed in prolonged exposure to the sensors. In a use case such as wound monitoring, wherein the sensor may be placed for over 7 days on the wound bed41,42, the cumulative ionic leaching can elicit significant cellular death.
In summary, our results suggest that understanding the toxicity mechanism of ions leached from printed conductive inks is crucial for developing biocompatible sensors. The lack of literature on cytotoxic properties of printed conductive inks drove us to explore any possible ion leaching and their subsequent toxicity pathways. While previous efforts had focused on the toxicity of certain ions, the ion leaching from printed conductive inks had not yet been explored. This research characterized two widely used commercially available inks (silver and carbon) for any potential ionic leaching using ICPS analysis. Results suggest that non-encapsulated screen-printed silver inks lead to a high concentration of leached Ag+ ions (> 600 ppb) eliciting a highly toxic response (cell viability < 70%) for concentrations > 0.5 mg ml-1. This is concerning in cases where prolong exposure is mandatory for monitoring, which is not limited to wearables but also to implantable sensors. However, our study further shows that the leaching can be optimally reduced by designing the sensors to have an encapsulating layer. A 50% decrease in the ion concentration was observed when the inks were encapsulated with TPU polymer. The ratio of encapsulation directly influences on the flexibility of the sensors therefore there is a limitation on how much coating can be applied. In case of carbon leaching, it was observed to be minimal thus causing negligible cytotoxic response (cell viability > 80%). The toxicity pathway assessment results indicate that Ag+ ion toxicity is induced primarily through the apoptotic pathway. The ROS induction triggers cellular damage leading to apoptosis. This pathway can highlight some of the latest reports of allergic and inflammatory responses caused when wearing smartwatches (leaching of nickel). Our study thus indicates that identifying the leaching of a potential ion from a conductive ink or electrical components are critical in assessing and elicit a toxic response. Further there is a need towards development of sustainable encapsulating agents and flexible electronics which can significantly lower the inflammatory response. This study is expected to encourage the exploration of suitable conductive inks and explore the dynamic and interfacial interaction of the wearable sensors with cellular systems to guide the future development of biocompatible devices.
Fabrication of wearable electrochemical sensors
The wearable electrochemical sensors used for detection of uric acid to monitor wound healing was used in our study. The sensor was prepared in-house using a multilayered screen-printing approach. Conductive carbon (DuPont Intexar PE671) and silver/silver chloride (Ag/AgCl, Creative Materials, 127-48E) inks were sequentially printed onto a thermoplastic polyurethane (TPU, TE-11C DuPont) substrate to fabricate the three-electrode electrochemical sensors. The printing was performed using a hand-operated screen-printing setup and involved the following steps: (i) The electrode pattern mask was cut out on an adhesive vinyl using a Silhouette cameo 3 die cutter. (ii) The mask was carefully placed on a polyester screen (mesh size: 200). (iii) Ag/AgCl ink was transferred to the TPU substrate through a hand-held squeegee angled at 45°. (iv) The printed layer was cured for 15 min at 80 °C in a convection oven. (v) A layer of carbon ink was printed on top of the cured Ag/AgCl ink to fabricate the working and counter electrodes. (vi) The printed layer was cured for 15 min at 80 °C in a convection oven. These fabricated sensors were termed non-encapsulated sensors. An additional step was carried out for the fabrication of encapsulated sensors wherein the above-printed electrodes were further encapsulated with a TPU layer. A layer of TPU was heat pressed over the electrode traces leaving the working area and electrode pads exposed. These electrodes are hereafter referred to as encapsulated sensors. An exploded view of the different sensor layers and a photograph of a sensor array is shown in Fig. 6a and b respectively.
The keratinocyte cell line (HaCaT) was provided by Dr. Marcus Cooke at the FIU Department of Environmental Health Sciences, Miami, Florida. The cell line was cultured and maintained in Dulbecco's Modified Eagle's Medium (DMEM, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, USA) and 1% antibiotics (penicillin/streptomycin, Gibco, USA). The cell line was maintained in an atmosphere of 5% CO2 at 37 °C with 70% humidity.
Time-dependent quantitation of ionic leaching
Preventing direct exposure to metallic ions on the skin is important to prevent any cell impairment. We hypothesized that the inks used (0.5 µg ml-1) in sensor fabrication may potentially result in the leaching of metal ions. To examine the leaching of Ag+ ions and carbon atoms from the printed Ag/AgCl and carbon inks respectively, the encapsulated and non-encapsulated sensors were incubated in 10 ml of deionized water for 1, 3, 5, and 7 days. The Ag+ ion dissolution was analyzed using inductively coupled plasma mass spectrometry (Thermo iCAP TQ triple Quadrupole) and the total dissolved carbon content (C) was measured using a total organic carbon analyzer (Shimazu TOC-V Analyzer). All the measured values were read against a calibration curve obtained from a set of standards.
Assessment of correlation of ionic leaching to cytotoxicity
The cell viability after exposure to the printed conductive inks at different ink concentrations was probed using the 3-(4, 5 dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, USA) assay. HaCaT cells were cultured up to a confluence of 75% and then trypsinized using a 0.25% trypsin solution (Gibco, USA). Cells were harvested and counted using a hemocytometer, and approximately 5000 cells/well were inoculated in a 96-well plate. The plate was incubated at 37ºC in 5% CO2 for 24 h, following which the cells were subjected to varying concentrations of the conductive inks (0.125 – 2 mg ml-1) for 24 h. Cells not exposed to any ink were used as the negative control. After the exposure, 10 µL of MTT reagent (15 µg ml-1) prepared in 1 × phosphate-buffered solution (PBS) (Gibco, USA) was added to each well. The plate was then incubated at 37 °C for 4 h, followed by the addition of 200 µL of dimethyl sulfoxide (DMSO, Sigma-Aldrich, USA) to each well. DMSO aids in dissolving the formazan crystals resulting in purple color. The cell viability was assessed by measuring the absorbance at 570 nm using a plate reader (ThermoFisher Sc. Multiskan™ FC microplate reader).
Assessment of toxicity mechanism via early generation of reactive oxygen species (ROS)
The total ROS generation in the cells on exposure to the sensors was assessed using the ROS generation Assay Kit, ThermoFisher. HaCaT cells were cultured up to a confluence of 75% and then trypsinized using a 0.25% trypsin solution (Gibco, USA). Cells were harvested and counted using a hemocytometer, and approximately 5000 cells/well were inoculated in a 96-well plate. The plate was incubated at 37ºC in 5% CO2 for 24 h, following which the cells were subjected to the encapsulated and non-encapsulated sensors for 24 h. The exposed cells were then fixed with 4% paraformaldehyde, washed with 1 × PBS, and permeabilized using 0.1% Triton-X. After washing, the cells were stained with rhodamine phalloidin for cytoskeleton staining and Hoechst 33,342 for nuclear staining. Cells were imaged at 20 × using a Axio Scope.A1 fluorescence microscope (Zeiss, USA).
Assessment of molecular mode of cell death
Cellular apoptosis on exposure to the sensors was assessed by monitoring the activation of Caspase-3/7. The Caspase-3/7 assay (CellEvent™ Caspase-3/7 Green Detection Reagent, ThermoFisher) consists of a fluorogenic substrate, that has a four amino acid peptide (DEVD) conjugated to a DNA binding dye. On activation of Caspase-3/7 in the apoptotic cell, the DEVD peptide is cleaved producing 6-aminoluciferin, and the dye binds to DNA, producing a bright fluorescence response. The intensity of the response is proportional to the amount of Caspase-3/7 activity. The assay is highly specific for caspase-3/7 activation and can be used to monitor their activation with live-cell fluorescence imaging. Since the cleaved reagent labels the nuclei of caspase 3/7–positive cells, the stain can be used to assess the nuclear morphology, including condensed nuclei which is typical of late-stage apoptosis. HaCaT cells were cultured up to a confluence of 75% and then trypsinized using 0.25% trypsin. Cells were harvested and counted using a hemocytometer, and approximately 5000 cells/well were inoculated in a 96-well plate, followed by exposure to the encapsulated and non-encapsulated sensors for 24 h. Subsequently, 100 μL of Caspase-3/7 reagent (5 μM) was added to each well. After incubation for 30 min at 37 °C, the cells were fixed with 4% paraformaldehyde, washed with 1 × PBS, and permeabilized using 0.1% Triton-X. Fluorescence images were captured at 20 × using a Axio Scope.A1 microscope (Zeiss, USA).
All the experiments were carried out in triplicate (n = 3) and all the values are represented as mean ± standard deviation (SD). Statistical analysis was carried out using the GraphPad Prism program (GraphPad Software, USA).
All data generated or analyzed during this study are included in this published article.
K Khatsenko Y Khin H Maibach 2020 Allergic contact dermatitis to components of wearable adhesive health devices Dermatitis 31 283 286
N Hamnerius M Mowitz 2020 Intense skin reaction to a new glucose monitoring and insulin pump system Contact Dermat. 83 524 527
MG Ahlström JP Thyssen M Wennervaldt T Menné JD Johansen 2019 Nickel allergy and allergic contact dermatitis: a clinical review of immunology, epidemiology, exposure, and treatment Contact Dermat. 81 227 241
F Torres M Das Graças M Melo A Tosti 2009 Management of contact dermatitis due to nickel allergy: an update Clin. Cosmet. Investig. Dermatol. CCID 2 39
M Santhiago 2017 Flexible and foldable fully-printed carbon black conductive nanostructures on paper for high-performance electronic, electrochemical, and wearable devices ACS Appl. Mater. Interfaces. 9 24365 24372
B Tian 2019 All-printed, low-cost, tunable sensing range strain sensors based on Ag nanodendrite conductive inks for wearable electronics J. Mater. Chem. C 7 809 818
X Liang 2020 Stable and biocompatible carbon nanotube ink mediated by silk protein for printed electronics Adv. Mater. 32 2000165
H Hong J Hu X Yan 2019 UV curable conductive ink for the fabrication of textile-based conductive circuits and wearable UHF RFID tags ACS Appl. Mater. Interfaces. 11 27318 27326
Yu Liao 2019 Highly conductive carbon-based aqueous inks toward electroluminescent devices, printed capacitive sensors and flexible wearable electronics RSC Adv. 9 15184 15189
B Sciacca J Groep van de A Polman EC Garnett 2016 Solution-grown silver nanowire ordered arrays as transparent electrodes Adv. Mater. (Deerfield Beach, Fla.) 28 905 909
F McGrath 2021 Structural, optical, and electrical properties of silver gratings prepared by nanoimprint lithography of nanoparticle ink Appl. Surf. Sci. 537 147892
N Karim S Afroj S Tan KS Novoselov SG Yeates 2019 All inkjet-printed graphene-silver composite ink on textiles for highly conductive wearable electronics applications Sci. Rep. 9 1 1 10
N Karim 2017 All inkjet-printed graphene-based conductive patterns for wearable e-textile applications J. Mater. Chem. C 5 11640 11648
J Kastner 2017 Silver-based reactive ink for inkjet-printing of conductive lines on textiles Microelectron. Eng. 176 84 88
J Liang K Tong Q Pei 2016 A water-based silver-nanowire screen-print ink for the fabrication of stretchable conductors and wearable thin-film transistors Adv. Mater. 28 5986 5996
D Li X Liu X Chen W-Y Lai W Huang 2019 A simple strategy towards highly conductive silver-nanowire inks for screen-printed flexible transparent conductive films and wearable energy-storage devices Adv. Mater. Technol. 4 1900196
FF Larese 2009 Human skin penetration of silver nanoparticles through intact and damaged skin Toxicology 255 33 37
M Wang X Lai L Shao L Li 2018 Evaluation of immunoresponses and cytotoxicity from skin exposure to metallic nanoparticles Int. J. Nanomed. 13 4445
Monteiro-Riviere, N. A. & Riviere, J. E. Interaction of nanomaterials with skin: Aspects of absorption and biodistribution. https://doi.org/10.1080/174353909029068033, 188–193 (2009).
P He 2019 Screen-printing of a highly conductive graphene ink for flexible printed electronics ACS Appl. Mater. Interfaces. 11 32225 32234
X Cao 2014 Screen printing as a scalable and low-cost approach for rigid and flexible thin-film transistors using separated carbon nanotubes ACS Nano 8 12769 12776
X Yuan X Zhang L Sun Y Wei X Wei 2019 Cellular toxicity and immunological effects of carbon-based nanomaterials Part. Fibre Toxicol. 16 1 27
W Li S Yang A Shamim 2019 Screen printing of silver nanowires: balancing conductivity with transparency while maintaining flexibility and stretchability npj Flexible Electronics 3 1 8
KK Krawczyk 2021 Self-reducing silver ink on polyurethane elastomers for the manufacture of thin and highly stretchable electrical circuits Chem. Mater. https://doi.org/10.1021/acs.chemmater.0c04025
N Hadrup AK Sharma K Loeschner 2018 Toxicity of silver ions, metallic silver, and silver nanoparticle materials after in vivo dermal and mucosal surface exposure: A review Regul. Toxicol. Pharmacol. 98 257 267
Y-M Cho 2018 Size-dependent acute toxicity of silver nanoparticles in mice J. Toxicol. Pathol. 31 73 80
AM Badawy El 2010 Surface charge-dependent toxicity of silver nanoparticles Environ. Sci. Technol. 45 283 287
P Bhushan Y Umasankar JD Hutcheson S Bhansali 2019 Toxicity assessment of wearable wound sensor constituents on keratinocytes Toxicol. In Vitro 58 170 177
P Limpiteeprakan S Babel 2016 Leaching potential of silver from nanosilver-treated textile products Environ. Monit. Assess. 188 3 1 12
ZJ Shepard EM Lux VA Oyanedel-Craver 2020 Performance of silver nanoparticle-impregnated ovoid ceramic water filters Environ. Sci. Nano 7 1772 1780
TM Benn P Westerhoff 2008 Nanoparticle silver released into water from commercially available sock fabrics Environ. Sci. Technol. 42 4133 4139
JN Smith 2018 All that is silver is not toxic: Silver ion and particle kinetics reveals the role of silver ion aging and dosimetry on the toxicity of silver nanoparticles Part. Fibre Toxicol. 15 1 12
C Levard 2013 Effect of chloride on the dissolution rate of silver nanoparticles and toxicity to E. coli Environ. Sci. Technol. 47 5738 5745
C Levard 2013 Sulfidation of silver nanoparticles: natural antidote to their toxicity Environ. Sci. Technol. 47 13440 13448
KJ Groh 2015 Critical influence of chloride ions on silver ion-mediated acute toxicity of silver nanoparticles to zebrafish embryos Nanotoxicology 9 81 91
M Mosallaei J Jokinen M Kanerva M Mäntysalo 2018 The effect of encapsulation geometry on the performance of stretchable interconnects Micromachines 9 645
ZW Zhong RWL Tang SH Chen XC Shan 2019 A study of screen printing of stretchable circuits on polyurethane substrates Microsyst. Technol. 25 339 350
OH Huttunen 2019 Roll-to-roll screen-printed silver conductors on a polydimethyl siloxane substrate for stretchable electronics Ind. Eng. Chem. Res. 58 19909 19916
A Herman 2017 Allergic contact dermatitis caused by isobornyl acrylate in Freestyle® Libre, a newly introduced glucose sensor Contact Dermat. 77 367 373
J Ulriksdotter C Svedman M Bruze M Mowitz 2022 Allergic contact dermatitis caused by dipropylene glycol diacrylate in the Omnipod® insulin pump* Br. J. Dermatol. 186 334 340
P Bhushan 2019 Biosensor for monitoring uric acid in wound and its proximity: a potential wound diagnostic tool J. Electrochem. Soc. 166 B830 B836
S RoyChoudhury 2019 Nanocomposite bienzymatic sensor for monitoring xanthine in wound diagnostics J. Electrochem. Soc. 166 B3295 B3301
This work was funded by the NSF Nanosystems Engineering Research Centre for Advanced Self-Powered Systems of Integrated Biosensors and Technologies (ASSIST, EEC- 1160483). The authors would also like to acknowledge Advanced Materials Engineering Research Institute and CAChE-Nutrient Analysis Core Facility for their assistance with material characterization.
The authors declare no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Bhushan, P., Kamat, V., Abrol, I. et al. Bio-acceptability of wearable sensors: a mechanistic study towards evaluating ionic leaching induced cellular inflammation. Sci Rep 12, 10782 (2022). https://doi.org/10.1038/s41598-022-13810-0