When exposed to nutrient excess and insulin resistance, pancreatic β-cells undergo adaptive changes in order to maintain glucose homeostasis. The role that growth control genes, highly expressed in early pancreas development, might exert in programming β-cell plasticity in later life is a poorly studied area. The imprinted Igf2 (insulin-like growth factor 2) gene is highly transcribed during early life and has been identified in recent genome-wide association studies as a type 2 diabetes susceptibility gene in humans. Hence, here we investigate the long-term phenotypic metabolic consequences of conditional Igf2 deletion in pancreatic β-cells (Igf2βKO) in mice. We show that autocrine actions of IGF2 are not critical for β-cell development, or for the early post-natal wave of β-cell remodelling. Additionally, adult Igf2βKO mice maintain glucose homeostasis when fed a chow diet. However, pregnant Igf2βKO females become hyperglycemic and hyperinsulinemic, and their conceptuses exhibit hyperinsulinemia and placentomegalia. Insulin resistance induced by congenital leptin deficiency also renders Igf2βKO females more hyperglycaemic compared to leptin-deficient controls. Upon high-fat diet feeding, Igf2βKO females are less susceptible to develop insulin resistance. Based on these findings, we conclude that in female mice, autocrine actions of β-cell IGF2 during early development determine their adaptive capacity in adult life.
Glucose homeostasis relies on pancreatic β-cells’ ability to adapt their insulin output to meet the sensitivity of tissues such as the liver, skeletal muscle and fat to insulin. Insulin production can be augmented in two main ways: firstly, by increasing the amount produced by each β-cell, or secondly through β-cell hyperplasia (i.e. expanding β-cell mass via cell proliferation). Failure of these compensatory responses in the face of insulin resistance, such as during obesity, pregnancy or ageing can lead to the development of diabetes1. So far, several extrinsic stimuli that control the adaptive expansion of β-cell mass, as well as processes intrinsic to β-cells, which mediate their response to an increased demand for insulin, have been identified2,3. The intrauterine milieu, which is determined by both genetic and non-genetic factors, appears to be critical for normal β-cell development and future adaptability to metabolic stress across the lifecourse4. Recent genome-wide association studies (GWAS) have identified several loci linked with decreased fetal growth and increased risk for type 2 diabetes (T2D), as well as alleles associated with higher birth weight and higher T2D risk5. Among these, three loci are located in regions regulated by genomic imprinting, including INS-IGF2, RB1 and DLK16.
Insulin-like growth factor 2 (IGF2) is a major growth factor during fetal life7. Igf2 is transcribed from the paternally inherited allele in most tissues8, and its expression in all tissues is dramatically down-regulated in mice around weaning9. In human, IGF2 expression also declines with age, although significant activity is retained during adult life9,10. Previous studies suggested that IGF2 is required for β-cell development and function. However, much of this evidence stems from in vitro studies or analyses performed with gain-of-function rodent models, and can be summarised as follows: (1) rat β-cells overexpressing Igf2 from a transgene were protected from interleukin-1β (IL-1β) cytokine-induced apoptosis ex vivo11 and islets isolated from neonatal rats were protected from cytokine-induced apoptosis when cultured in the presence of IGF212; (2) diabetic rats transplanted with islets overexpressing Igf2 had improved glucose tolerance compared to diabetic rats transplanted with standard islets, and β-cell replication rate was higher in Igf2-overexpressing islets compared to control islets after transplantation13; (3) Goto-Kakizaki (GK) rats that develop spontaneous diabetes had decreased IGF2 protein levels in their pancreatic bud, prior to the onset of the β-cell mass reduction14; (4) transgenic mice with global Igf2 overexpression had larger pancreatic islets at the end of gestation, with increased cell replication and reduced apoptosis15; (5) transgenic mice with overexpression of Igf2 in β-cells under the control of rat insulin I promoter had a threefold expansion of β-cell mass, with disrupted islet morphology, hyperglycaemia when fed a standard chow diet and overt diabetes when fed high fat diet (HFD)16; and (6) conditional Igf2 deletion in pancreatic β-cells led to reduced glucose-stimulated insulin secretion (GSIS) in 24–26 weeks-old females fed chow diet, and lower GSIS in both sexes after 18 weeks of HFD feeding, associated with reduced β-cell mass in females17.
We recently characterised Igf2 expression in the main pancreatic cell lineages during perinatal life18. We observed that at embryonic day 16 (E16) there was significant Igf2 expression in pancreatic β-cells, which then declines rapidly after birth, being approximately two orders of magnitude lower from weaning onwards. However, we also found that the main pancreatic cell lineage expressing Igf2 throughout perinatal life was that of mesenchyme-derived cells18. Accordingly, conditional Igf2 deletion in the pancreatic mesenchyme, driven by Nkx3.2-Cre, led to a smaller pancreas, with reduced acinar and β-cell mass and altered glucose homeostasis when mutant females became pregnant18. This study highlighted an important role for pancreatic mesenchyme IGF2 in paracrine growth signalling, including the β-cell. One area that remains poorly understood is if, and how, fetal growth control genes expressed in the β-cell, such as Igf2, are involved in programming of diabetes susceptibility in later life. Data from Modi et al.17, and our own data on conditional Igf2 deletions in the pancreatic endocrine lineage (Ptf1a-Cre)18, suggest that in normal physiological conditions, deletion of Igf2 from β-cells or its precursors, have little effect on β-cell biology. However, several questions remain to be elucidated: (1) Is β-cell remodelling, which occurs in the early postnatal period19, affected by the embryonic deletion of β-cell Igf2? (2) Is β-cell mass expansion20 and/or glucose homeostasis impaired in pregnant mothers that lack Igf2 in their β-cells? If yes, does it have consequences for the growth and metabolism of their offspring? (3) Does congenital obesity21 or HFD-induced obesity22, together with embryonic deletion of β-cell Igf2 result in early programming of diabetes? By addressing these questions, we therefore aim at testing the possibility that autocrine actions of IGF2 in pancreatic β-cells during early life may program their ability to adapt to physiological challenges or conditions that cause insulin resistance throughout the life-course.
Autocrine IGF2 is not required for early postnatal β-cell remodelling and function
We achieved conditional deletion of the paternal Igf2 allele in pancreatic β-cells (Igf2βKO) (Fig. 1a) by crossing heterozygous Igf2 floxed male mice (Igf2+/fl)18 with homozygous RIP-Cre female mice that carry a Cre transgene under the control of the rat Ins2 (insulin 2) promoter23. We used a recombinase-inducible YFP reporter under the control of the Rosa26 locus (Rosa26YFP-stopfl/fl)24, which enabled us to isolate pancreatic β-cells using Fluorescence Activated Cell Sorting (FACS) to verify for efficiency and specificity of Igf2 deletion. (Supplemental Fig. S1a–e). Paternal Igf2βKO resulted in ~ 96% reduction of its mRNA levels in pancreatic β-cells isolated by FACS at postnatal day 2 (P2) (Fig. 1b). In contrast, Igf2 mRNA levels were similar between Igf2βKO and littermate controls in several other tissues tested, including in the hypothalamus (Fig. 1b), in which we observed only very few isolated YFP + cells (Supplemental Fig. 1f), in agreement with previous studies25.
Analysis of recently published transcriptomes of bulk β-cells isolated by FACS at several developmental time points, spanning from E17.5 to P6026, showed that Igf2 mRNA levels are relatively much higher in perinatal life and decline rapidly few days after birth (Fig. 1c), in line with our own previous results18. To assess whether Igf2βKO alters the dynamics of the early postnatal wave of β-cell remodelling, we first studied rates of cell proliferation at P5, P10 and P14 after intra-peritoneal administration of EdU (5-ethynyl-2′-deoxyuridine)27 for 6 h. The percentage of proliferating β-cells (EdU+/INS+) was significantly affected by age, decreasing from ~ 6.6% at P5 to ~ 2.6% at P14 in controls (Fig. 1d). We observed a small genotype-dependent reduction of β-cell proliferation rate across the three ages studied (Fig. 1d). Rates of β-cell apoptosis (TUNEL+/INS+) peaked around P10 (Fig. 1e), as previously reported19. However, there was no genotype-dependent difference in the frequency of apoptotic β-cells (Fig. 1e). Additional parameters measured in early postnatal life, including β-cell mass (Fig. 1f), total pancreatic insulin content (Fig. 1g) and non-fasting blood glucose levels (Fig. 1h) were similar between Igf2βKO mutants and littermate controls. Altogether, our results show that the autocrine actions of IGF2 are not required for β-cell remodelling or function in early postnatal life.
Igf2 βKO does not alter glucose homeostasis in adult mice fed standard chow diet
In order to estimate the impact of Igf2βKO on body growth and glucose homeostasis in adult life, we followed up a cohort of mutant mice and littermate controls fed with a regular chow diet from weaning up to the age of 48 weeks. Both sexes displayed similar age-related weight gain, without any significant genotype-related difference (Fig. 2a). We monitored the impact on glucose homeostasis in both sexes by performing intra-peritoneal glucose tolerance tests (ipGTTs) after an overnight fast at the ages of 4, 17 and 39 weeks (Fig. 2b,c). Within the age range of our study, we observed significant age-related glucose intolerance only in males, as assessed by area under curve (AUC) analyses (Fig. 2c). However, neither sex had significant differences in AUC related to genotype (Fig. 2b,c). We also performed standard serum biochemistry profiling at the end of the study (i.e., at the age of 48 weeks) after overnight fasting. The lipid profile showed sex-related differences, with levels of triglycerides, free fatty acids and cholesterol being significantly higher in males than in females but levels of these lipids were similar between the two genotypes in both sexes (Supplemental Fig. 2a). Corticosterone levels were lower in males than in females, but similar between mutants and controls (Supplemental Fig. 2b). Additionally, there was no significant sex-related or genotype-related differences in glucose and insulin levels after an overnight fast (Supplemental Fig. 2c). Therefore, we conclude that ablation of Igf2 in pancreatic β-cells does not alter glucose or insulin homeostasis during adult life in mice fed a regular chow diet.
Pregnant Igf2 βKO females have altered glucose homeostasis, with an impact on fetal development
After establishing that the autocrine actions of pancreatic β-cell IGF2 are not required for the normal function of these cells, from in utero development up until 39 weeks of age under physiological conditions, the next goal was to find out if pancreatic β-cell IGF2 was necessary for β-cell plasticity during pregnancy, when demands for insulin increase20. To study the impact of Igf2βKO on maternal adaptation to pregnancy, 6-to-8 week old Igf2βKO and control females were timed-mated with wild-type males and analyses were performed on embryonic days E15 (i.e. at the peak of pregnancy-associated insulin resistance20) and E19 (near term). Total body weight gain during pregnancy, litter sizes and pancreas weights were indistinguishable between the two genotypes (Supplemental Fig. 3a–c). Non-fasting glucose levels decreased as gestation progressed, but remained comparable between the two genotypes (Fig. 3a). However, while in control females non-fasting circulating insulin levels increased at E15 and then returned to levels similar to non-pregnant females by E19, insulin levels remained elevated in mutant females during late gestation, being significantly higher at E19 compared to control females (Fig. 3a). Hyperinsulinemia in pregnant Igf2βKO females was not associated with increased pancreatic β-cell mass, which was similar to controls at E19 (Fig. 3b). We did not find evidence of impaired insulin sensitivity in periphery driving the observed hyperinsulinemia at E19, with levels of well-established metabolic biochemical markers such as leptin, adiponectin and resistin28 being similar between the two genotypes (Supplemental Fig. 3d). The expected pregnancy-associated patterns (i.e. increased levels of leptin and resistin and decreased levels of adiponectin compared to non-pregnant control females) were observed (Supplemental Fig. 3d). At E15, after six hours of fasting, the pregnant Igf2βKO mouse exhibited hyperglycaemia compared to pregnant controls, while fasting insulin levels remained comparable between the two genotypes (Fig. 3c). However, oral glucose tolerance tests (OGTTs) did not show significant differences between the two genotypes at E15 (Fig. 3d).
Next, we assessed whether maternal hyperglycaemia and hyperinsulinemia observed in Igf2βKO females in late pregnancy had any impact on the growth and glucose metabolism of their conceptuses. Fetal weights were similar at both E15 and E19 (Fig. 3e). However, at E19, placental weights in Igf2βKO pregnancies showed a small but statistically significant increase (5%) compared to those in control pregnancies (Fig. 3e). Levels of glucose in the fetal blood were indistinguishable in litters of Igf2βKO versus control mothers at both E15 and E19 (Fig. 3f). However, levels of insulin measured at E19 in individual fetuses were almost double in litters of Igf2βKO mothers (Fig. 3e). Overall, our results show that Igf2βKO females have altered glucose homeostasis during pregnancy, associated with increased maternal and fetal insulinemia and larger placentae near term.
Igf2 βKO females are less susceptible to develop HFD-induced insulin resistance in adulthood
We next investigated whether HFD feeding can elicit altered glucose homeostasis in Igf2βKO mice. To this aim, we fed a cohort of Igf2βKO and control littermate mice with a standard chow diet from weaning (3 weeks) until the age of 28 weeks, after which half of the mice were fed HFD (60% kcal from fat) for the following 13 weeks, while the remaining half were kept on the control chow diet. Measurements of body composition by TD-NMR at the ages of 8 and 28 weeks revealed significant age-related accrual in both fat and lean mass, without any significant genotype-related effects in either of the two sexes (Supplemental Fig. 4a,b). Between the ages of 28 and 40 weeks, both sexes gained significantly more weight when fed HFD (Fig. 4a). TD-NMR measurements at the age of 40 weeks showed a significant increase in fat mass in the mice fed HFD for both sexes (Fig. 4b). Lean mass was significantly increased by HFD only in females (Fig. 4c). Neither fat mass, nor lean mass showed any significant differences related to the genotype (Fig. 4b,c). At the age of 40 weeks (i.e. 12 weeks after the HFD was introduced to half of the animals), all mice were subjected to insulin tolerance tests (ITTs), followed a week apart by OGTTs. ITTs performed in females did not show any significant diet-related differences. However, there was a significant impact of the genotype, Igf2βKO females being less susceptible to develop insulin resistance than their littermate controls, as indicated by AUC analyses after correction to basal levels (Fig. 4d). On the contrary, ITTs performed in males revealed increased diet-associated insulin resistance, without any significant impact of the genotype (Fig. 4e). OGTTs showed that both sexes displayed a small but significant glucose intolerance induced by HFD, without any impact of the genotype (Fig. 4f,g). To investigate further the improved insulin sensitivity observed in Igf2βKO females, we measured GSIS. Insulin levels were higher in females fed HFD at baseline and increased significantly more during OGTT (AUC P diet = 0.0142), without any significant impact of the genotype (Fig. 4h). β-cell mass, measured at the end of the experiment, was significantly higher in mice fed HFD for both sexes, without any difference related to the genotype (Fig. 4i). Overall, these data indicate that, when fed HFD in adulthood, Igf2βKO mice retain the ability to adapt by expanding β-cell mass, and that Igf2βKO females are more insulin sensitive than their littermate controls.
Igf2 βKO leads to exacerbated hyperglycaemia in females with congenital leptin deficiency
We next assessed the impact of Igf2βKO mutation in a model of genetic obesity and marked insulin resistance due to congenital leptin deficiency (Lepob/ob). Lepob/ob mice display severe obesity first recognisable at about four weeks of age29. We generated litters with four genotypes (controls, Igf2βKO, Lepob/ob and Lepob/ob; Igf2βKO—Supplemental Fig. 5) and performed phenotypic analyses in young adult life (at the age of 8 weeks). For both sexes, mice in the ob/ob background were significantly obese, as expected, but there was no impact of Igf2βKO mutation on overall body weight (Fig. 5a). Fasting glucose levels were also elevated in mice on the ob/ob background in both sexes (Fig. 5b). However, in females, but not in males, there was an additional significant increase in fasting glucose levels in Lepob/ob; Igf2βKO compared to Lepob/ob mice (Fig. 5b). For both sexes, ipGTTs showed significant glucose intolerance in mice on the ob/ob background (Fig. 5c). However, in both males and females, AUCs corrected to baseline were not statistically different between Lepob/ob and Lepob/ob; Igf2βKO mice (Fig. 5c). In females, we observed significant insulin resistance in mice on the ob/ob background, but with no further impact caused by the Igf2βKO mutation (Fig. 5d). In males, all four genotypes had similar levels of insulin sensitivity, as assessed by AUC analyses after normalising to the baseline glucose levels (Fig. 5d). Together, these results demonstrate that lack of Igf2 expression in pancreatic β-cells is detrimental in obese females with congenital leptin deficiency, leading to exacerbated hyperglycaemia in young adult life.
This study strongly suggests that autocrine IGF2 actions in pancreatic β-cells during early life program their capacity to adapt under circumstances that require increased insulin production in adult life, in females. We first show that under normal physiological conditions Igf2βKO is compatible with normal pancreatic β-cell development, remodelling and function during early postnatal life, in both males and females. In the absence of a metabolic challenge, Igf2βKO is also compatible with normal glucose homeostasis during adult life.
We then exposed Igf2βKO and littermate control mice to several models of increased metabolic demand leading to insulin resistance and therefore need for increased insulin release. Firstly, we challenged Igf2βKO via pregnancy, a physiological condition associated with reduced insulin sensitivity in the latter stages20. After six hours fasting, Igf2βKO females became hyperglycaemic at E15, with normal levels of insulin and normal clearance of glucose from periphery during OGTT. These observations suggest that at this stage of pregnancy, Igf2βKO females cannot reach the level of β-cell compensation required to maintain glucose homeostasis. We did not perform fasting glucose/insulin measurements or OGTTs at the end of gestation (E19). Therefore, we can’t rule out that, in the fasting state, Igf2βKO females are hyperglycaemic at both time points or that they are glucose intolerant only at E19. However, we found that non-fasted Igf2βKO females were normoglycaemic at both time points and exhibited hyperinsulinemia, in particular at E19, suggesting continued β-cell maladaptation. The effects observed in offspring provide evidence supporting the proposed maternal β-cell maladaptation. Accordingly, and although the conceptuses had normal development and growth up until at least E15, at E19 they associated placentomegaly and hyperinsulinemia. We did not identify the signal that triggers the observed changes in the conceptus. Given the known roles of placenta in mediating nutrient transfer between the mother and the fetus30, as well as the role played by several placental hormones in mediating maternal adaptations during pregnancy31, we propose that the observed changes in the mother and the fetus are interconnected. We speculate that the fetus is sensing intermittent episodes of maternal hyperglycaemia and adapting through increased production of insulin. In turn, fetal hyperinsulinemia elicit placentomegalia, with excessive secretion of placental hormones, perhaps leading to maternal hyperinsulinemia at the end of gestation. It would be interesting to assess in future studies whether the hyperinsulinemia observed in the fetuses of Igf2βKO females at E19 is due to changes in fetal β-cell mass, or is caused by increased glucose-mediated insulin secretion, and has a long-lasting impact on their post-natal growth and metabolism. Similarly to our model, fetal hyperinsulinemia has been reported in liver-specific insulin receptor knockout mice (LIRKO), which results from sustained maternal hyperinsulinemia and transient increase in blood glucose concentrations during pregnancy32. Additionally, foetuses from mildly diabetic rat mothers (diabetes induced by streptozotocin administration prior to mating) show normal body weight, placentomegalia, higher pancreatic and plasma insulin concentrations at the end of gestation and enhanced insulin secretion by fetal pancreatic β-cells in response to glucose stimulation in vitro33. It would also be compelling to explore whether the metabolic changes observed during the first pregnancy in Igf2βKO female mice become more pronounced in subsequent pregnancies, e.g. leading to diabetes. Women with gestational diabetes (GDM) have increased risk of recurrent GDM because of their underlying β-cell impairment34. Furthermore, previous GDM is an important predicting factor for subsequent diagnosis of diabetes in later life35.
The next model of increased metabolic demand used in this study was the exposure to a diet with high content in fat (HFD), known to induce obesity and insulin resistance22. We observed that Igf2βKO females, but not the males, are more resistant to developing HFD-induced insulin resistance. However, the impact of Igf2βKO mutation on insulin sensitivity in females fed HFD is relatively mild, with no impact on other variables measured. Additional tests such as hyperinsulinemic-euglycaemic glucose clamps, which allow a direct assessment of insulin resistance and glucose uptake by peripheral tissues36, would further refine the impact of Igf2βKO on β-cell physiology in mice exposed to HFD. Our finding suggests the possibility of secreted signal(s) originating from pancreatic β-cells and affecting insulin sensitivity in the periphery. Our current knowledge of the β-cell secretome is limited37,38 but a very recent study uncovered the role of β-cell–derived exosomes that regulate peripheral insulin sensitivity in a paracrine manner via microRNA-26a (miR-26a)39. Future studies could explore the impact of autocrine IGF2 in regulating β-cell secretome.
An additional model of increased metabolic demand used in this study was that of congenital leptin deficiency22. In Lepob/ob mutants, Igf2βKO led to augmented hyperglycaemia but only in females. It is interesting to observe that in two models of increased metabolic demand (congenital leptin deficiency and pregnancy), Igf2βKO females exhibited hyperglycaemia, while in the third (HFD feeding) Igf2βKO females were normoglycaemic and more insulin sensitive than their controls. We speculate that these outcome differences may relate to the diets used (chow diets are high in carbohydrates and low in lipids, while the HFD used in this study is high in lipids and low in carbohydrates) that trigger divergent insulin actions, as recently reviewed40. Based on the observations made in this study, we suggest that autocrine IGF2 may be more critical under circumstances with increased metabolic demand in females.
A number of previous studies proposed several molecular mechanisms by which autocrine IGF2 controls β-cell physiology. These range from protection against β-cell apoptosis (via a GLP-1-mediated increase in IGF1R expression)41, to promoting β-cell proliferation during pregnancy (dependent on estrogen, which reduces the expression of miR-338-3p, leading to increased IGF1R expression)17 and to controlling fasting insulin secretion (via IGF1R-AKT2-FAK signalling)42. However, these molecular mechanisms were based on observations made in vitro in insulin-secreting cell lines or in primary β-cells or pancreatic islets cultured ex vivo and require in vivo testing to be substantiated.
In a previous study, Modi et al. reported a more substantial impact of autocrine IGF2 on β-cell function using the same approach of a conditional deletion of Igf2 in pancreatic β-cells17. Contrary to our observations, Modi et al. observed impaired β-cell expansion during pregnancy in Igf2βKO females. Likewise, when fed HFD, they recorded significantly worsened glucose tolerance in Igf2βKO females compared to controls. However, there are some significant differences between the experimental setup of the two studies, which may account for the phenotypic differences observed. First, we performed our experiments under a homogeneous C57BL/6 J genetic background. In contrast, Modi et al.17 used a mixed 129S6/C57BL/6 background. It is known that C57BL/6 and 129S6 strains exhibit notable differences in patterns of glucose homeostasis and insulin secretion under regular diet or when challenged with HFD43. Second, Modi et al.17 used Ins1-Cre to achieve deletion of Igf2 in pancreatic β-cells, while we used RIP-Cre. RIP-Cre may have some weak ectopic activity in the brain25. However, as shown in the results section, we did not see any significant change of Igf2 mRNA levels in the hypothalamus, and brain sections from offspring of RIP-Cre mice mated with Rosa26YFP-stopfl/fl reporter mice showed only very few and sporadic YFP + cells. Additionally, in our experiments both control and mutant mice were all heterozygous for RIP-Cre, with maternal inheritance of the transgene. This design thus rules out a contribution of the Cre transgene on the observed mutant phenotypes. In most experiments reported by Modi et al., only the mutants inherited an Ins1-Cre allele. Although hemizygosity for Ins1 gene has no phenotypic impact on glucose homeostasis44, Cre recombinase expression has the potential to lead to changes in the pancreatic β-cells’ physiology (albeit the Ins1-Cre line has been carefully verified for lack of activity in the central nervous system and transmission of Ins1-Cre has been reported to not affect glucose homeostasis up until the age of 12 weeks45). Third, differences in HFD composition, age at feeding and length of exposure may be responsible for some of the diverging phenotypic outcomes. Indeed, Modi et al. performed the ipGTT challenge after a longer exposure to HFD diet (18 weeks compared to 12–14 weeks in the experiments reported in our study) and they used a diet with 45% energy derived from fat (60% in our study). The ages of various cohorts of mice used in the two studies were also distinct. Overall, these experimental differences call for caution when trying to draw direct comparisons between the two Igf2βKO models. One of the common observations between our studies is that Igf2βKO females seem more susceptible to develop altered glucose homeostasis than Igf2βKO males when exposed to increased metabolic demand. Sex-related differences in β-cell function under stress conditions have been observed before in many clinical studies and in animal models of diabetes46. Estrogen acting on β-cells via ERα (estrogen receptor type α) promotes cell survival and insulin biosynthesis, and enhances GSIS through ERβ47. Interestingly, Igf2 is a known estrogen-responsive gene, at least in some tissues such as the hippocampus48, and sex-related expression differences during development in organs such as the brain49 have been documented. Mody et al. proposed an interplay between estrogens and the IGF2 autocrine actions in controlling β-cell mass and function in female mice. Our results further suggest that Igf2 may be one of the genes that mediate the protective actions of estrogen on pancreatic β-cells in females, under conditions of increased metabolic demand.
Given that IGF2 expression timing in mouse and human are different, is there any relevance for our findings regarding IGF2 actions in human β-cells? A significant number of genome-wide association studies (GWAS) have linked T2D with the human INS-IGF2 locus6,50, as well as with the IGF2BP2 locus51 that plays an important role in IGF2 mRNA translation52. A loss-of-function splice acceptor IGF2 variant was found to protect against T2D53. None of the above studies can point directly to a defect in β-cell function. However, some studies provide a more direct link. Human pancreatic islets express a hybrid protein INS-IGF2 that consists of the pre-proinsulin signal peptide, the insulin B-chain, and eight amino acids of the C-peptide in addition to 138 amino acids encoded by the IGF2 gene. INS-IGF2 expression was lower in pancreatic islets of T2D donors compared to controls54. Notably, INS (38%), INS-IGF2 (10%) and IGF2 (2%) were the top three most abundant transcripts expressed in human β-cells isolated from donors without diabetes55. Finally, some forms of insulinomas, endocrine pancreatic tumours that lead to severe forms of hyperinsulinemic hypoglycaemia, associated hypermethylation at the IGF2 differentially methylated region 2 (IGF2-DMR2), with IGF2 loss-of-imprinting and overexpression56.
Our study has a number of limitations. First, we cannot fully exclude that the RIP-Cre line used in our study has an impact on β-cell physiology that could influence the differences observed when comparing controls to Igf2βKO mutants. Other β-cell Cre drivers have been previously shown to induce impaired islet function due to the expression of a human growth hormone (hGH) minigene, which was frequently used to enhance transgene activity57. The RIP-Cre line used in this study does not contain the hGH minigene58. However, a more subtle impact on β-cell function may still exist. Second, we cannot separate, in our study, autocrine IGF2 actions in early life, when Igf2 mRNA levels are much higher, from those in adult life, when Igf2 mRNA levels are very low, but still detectable. A direct proof that the observed phenotypes are due to programming effects of autocrine IGF2 in early life, as suggested, would require the use of inducible β-cell-specific Cre lines that enable temporal control of Cre recombination59. Third, we cannot exclude a contribution of miR-483, a microRNA embedded within intron 4 of Igf2 and deleted in our model, to the phenotypes observed in Igf2βKO mutants. Previous in vitro data obtained in MIN6 insulin-secreting cells, has shown that miR-483 promotes insulin transcription and secretion by targeting SOCS3, a member of suppressor of cytokine signalling family60. Fourth, we did not explore the impact of Igf2βKO in the context of ageing, which is one of the well-known risk factors for type 2 diabetes development61. Aged mice are considered to be those older than 18 months, which has been suggested to be equivalent to 56 + in human years62. In the cohorts of mice fed normal chow or HFD, we did not extend the follow-up beyond 10 months. We cannot exclude that Igf2βKO would develop diabetes when significantly older. Fifth, for the cohort of mice fed HFD, the phenotypic studies were conducted after 12–14 weeks of exposure, similar to timelines reported in other studies using conditional deletions in pancreatic β-cells63,64,65. However, we cannot exclude that more prolonged HFD feeding is required for Igf2βKO mice to reach their maximum capacity for β-cell mass expansion, leading to more severe glucose intolerance compared to controls. Sixth, combinations of stress conditions, such as pregnancy associated with HFD feeding, or ageing and HFD feeding may be required to uncover β-cell functional defects induced by the Igf2βKO mutation. Lastly, the autocrine IGF2 actions may be masked by the paracrine actions of IGF2 produced by neighbouring cells. In our previous study we observed that mice lacking mesenchyme-derived IGF2 have reduced β-cell mass and develop glucose intolerance during pregnancy18. We also observed significant expression of Igf2 mRNA in the endothelial cells. Other endocrine cells within the islets of Langerhans may also affect β-cell plasticity via paracrine IGF2 actions. Combinations of conditional deletions in multiple cell types, aimed at reducing the paracrine IGF2 actions may uncover additional roles of autocrine IGF2 in pancreatic β-cells.
In summary, we report in this study that autocrine actions of IGF2, although not required for development of pancreatic β-cells, have a long-term impact on β-cell plasticity that becomes apparent in female mice under conditions associated with increased demand for insulin. Our results also highlight that even subtle defects in maternal pancreatic β-cell function can affect the normal development and physiology of the descendants, with potential implication for metabolic health in later life.
This study was carried out in compliance with the ARRIVE guidelines66. The research has been regulated under the Animals (Scientific Procedures) Act 1986 Amendment Regulations 2012 following ethical review and approval by the University of Cambridge Animal Welfare and Ethical Review Body (AWERB) and by the Ethical and Veterinary committees of Rey Juan Carlos University in Spain. All mouse experiments were approved and performed under PPL No. 80/2483 (study 2483/02/12), PPL No. 80/2484 (study 2484/38/12) and PPL No. 70/7594 (study 7594/4/15).
Mouse strains and husbandry
Igf2fl/+ mice were generated in our laboratory, as described18. C57BL/6J mice were purchased from Charles River (Kent, UK). Rosa26YFP-stopfl/fl reporter mice24 were kindly provided by Dr. Martin Turner (The Babraham Institute, Cambridge). RIP-Cre mice that carry a Cre transgene under the control of the rat Ins2 (insulin 2) promoter (RIP), which directs expression to insulin-positive β-cells from approximately E8.5–9 onwards23, were obtained from Central Biomedical Services (CBS Transgenic Services, University of Cambridge). Lepob/+ mice that have a spontaneous mutation in the Lep gene encoding leptin67 were available at the Universidad Rey Juan Carlos mouse facility. All lines were maintained onto an inbred C57BL/6J genetic background for > 10 generations prior to the experiments performed in this study.
Mice were fed a standard chow diet with 9% of kcal from fat (SDS, Essex, UK), or a high fat diet (HFD) containing 60% kcal from fat (D12492, Research diets Inc., New Brunswick, USA) and housed with a 12-h light/dark cycle in a temperature-controlled room (22 °C). Food and water were available ad libitum, except for periods of fasting when food was withdrawn. For timed matings, the day of detection of a vaginal plug was noted as embryonic day 1 (E1) and the day of birth was noted as post-natal day 0 (P0). Mice were weaned at 3 weeks of age and ear notches were used for visual identification and genotyping, which was performed using standard PCR or qPCR (quantitative PCR—in order to discriminate between RIPCre/+ and RIPCre/Cre) with primers listed in Supplementary Table 1. Genotyping for the mouse obese (ob) mutation at the Lep locus was performed by PCR and restriction fragment length polymorphism (RFLP) analysis, as described68.
Fluorescence-activated cell sorting (FACS)
P2 pups were sacrificed by decapitation. Then, pancreases were dissected under a dissection microscope and dissociated into single cells with trypsin–EDTA (Sigma Aldrich), at 37 °C, for 20 min. After washing with ice-cold PBS, cells were passed through 70 μm strainers and single-cell suspensions were sorted into YFP+ and YFP- fractions using an Aria-Fusion cell sorter (BD Bioscience). Dead cells were excluded based on forward and side scatter profiles and the uptake of 7AAD (7-Aminoactinomycin D dead cell stain, Life Technologies). Sorted YFP+ cells were pelleted by centrifugation and flash frozen using liquid nitrogen (N2), and then stored at -80 °C until use.
Total RNA was extracted from FACS-isolated β-cells and other organs using RNeasy Plus Kits (Qiagen—74134 and 74034). RNA concentration was measured by NanoDrop (Thermo Scientific) and quality was assessed in agarose gels. RNA extracted from FACS-isolated β-cells was quantified and assessed for quality using the RNA 6000 Pico Kit (Agilent—5067-1513) and an Agilent 2100 Bioanalyzer. Reverse transcription was performed using the RevertAid RT Reverse Transcription Kit (ThermoFisher—K1622). In the case of total RNA extracted from FACS-isolated β-cells, cDNA was produced using the QuantiTect Whole Transcriptome Kit (Qiagen) following manufacturer’s instructions. qRT-PCR was performed with the SYBR Green JumpStart Taq Ready Mix (Sigma—S4438) and custom-made primers (Supplementary Table 2) using an ABI Prism 7900 system (Applied Biosystems). Gene expression normalisation was performed against three housekeeping genes: Ppia (peptidylpropyl isomerase A or cyclophilin-A), Gapdh (glyceraldehyde 3-phosphate dehydrogenase) and Sdha (succinate dehydrogenase complex flavoprotein subunit A). Relative levels of expression were calculated using the 2−ΔΔCt method69.
Immunostainings, cell counting and β-cell mass analyses
Pancreases and brains were dissected (using a stereoscope for the early post-natal analyses), fixed in 4% paraformaldehyde in PBS overnight, dehydrated and then embedded in paraffin. Paraffin blocks were cut at 5 μm thickness, sections were then deparaffinised, rehydrated, stained and mounted with coverslips. Insulin and YFP stains and β-cell mass measurements using pancreas stereology were performed as previously described18. Measurements of β-cell proliferation were preceded by intraperitoneal injections with 50 µg of 5-ethynyl-2′-deoxyuridine (EdU)/g body weight, 6 h prior to tissue collection. EdU staining was done using the Click-iT EdU Alexa Fluor 488 Imaging Kit (Invitrogen—C10337), according to manufacturer’s instructions. TUNEL staining was performed using the In Situ Cell Death Detection Kit, TMR red (Sigma—012156792910), according to manufacturer’s protocol. For immunofluorescence stains, Hoechst33342 (Sigma—B2261) or DAPI (Sigma—D9542) were used to label the nuclei. Immunofluorescence image acquisition was performed as Z-stacks using a LSM510 Meta confocal laser scanning microscope (Carl Zeiss, Jena, Germany) and the ZEN 2009 software. Counting proliferating β-cells (EdU+/INS+) and apoptotic β-cells (TUNEL+/INS+) was performed using Volocity 6.3 (Improvision).
Body composition analysis was performed by time-domain nuclear magnetic resonance spectroscopy (TD-NMR) that measures total body fat mass and lean mass70. For this purpose, live and conscious mice were placed inside the Minispec Live Mice Analyser (Bruker Minispec Live Mice Analyser LF50).
Glucose and insulin tolerance tests
Oral glucose tolerance tests (OGTTs), intra-peritoneal glucose tolerance tests (ipGTTs) and insulin tolerance tests (ITTs) were performed on conscious mice after 6 h fasting (8am to 2 pm—for OGTTs performed on pregnant females, for OGTTs and ITTs on mice fed HFD, and for ITTs for the cross with Lepob/+ mice) or 16 h fasting (5 pm to 9am following day—for ipGTTs performed on adult mice fed chow diet and for the cross with Lepob/+ mice). Throughout these experiments, mice were kept in heated cages (32 °C) to facilitate blood collection from the tail vein. Blood samples, taken from the tail vein immediately before the start of each experiment, were used to measure glucose and/or insulin levels in the fasting state. For OGTT, glucose was administered by oral gavage at a dose of 2 mg/g body weight (adjusted per individual animal for the pregnancy experiment or fixed volume calculated for an averaged body weight for the HFD experiment). For ipGTTs, glucose was administered by i.p. injection at a dose of 1 mg/g body weight. For ITTs performed on mice fed HFD, insulin was administered by i.p. injection at a dose of 0.75mUI/g body weight for females and 1mUI/g body weight for males (fixed volume calculated for an averaged body weight). For ITTs performed for the cross with Lepob/+ mice, same dose of 0.75mUI/g body weight was used for both sexes. Throughout the experiments, glucose measurements were performed using a glucose meter and test strips (AlphaTRAK). The areas under the curve (AUCs) following OGTTs, ipGTTs or ITTs were calculated by the trapezoidal rule.
Plasma insulin and total pancreas insulin measurements
Blood samples for plasma insulin measurements were collected in heparinised capillary tubes during OGTT experiments at 0, 15, 30, 45 and 60 min. Tubes were kept on ice and spun at 4,000 RPM (rotations per minute) for 5 min. Plasma samples were flash frozen in liquid N2 and stored at -80 °C until analysis. For total pancreas insulin measurements, whole pancreases were flash frozen in liquid N2, then pulverised and re-suspended in cold acid–ethanol and stored at 4 °C for 48 h, with sonication every 24 h during the storage. Insulin levels in plasma and acid–ethanol supernatants were measured using ELISA kits (Meso Scale Discovery Mouse/Rat Insulin Assay Kit) at CBAL (Core Biochemical Assay Laboratory, Addenbrooke’s hospital). Total pancreas insulin content (ng) was normalised to the total pancreas wet weight (mg), measured at collection.
Serum glucose, triglycerides, free (non-esterified) fatty acids, and total cholesterol concentrations were measured using enzymatic assay kits. Briefly, glucose was measured based on an adaptation of the hexokinase-glucose-6-phosphate dehydrogenase method using a kit from Siemens Healthcare (product code DF30). Triglycerides were measured using an enzymatic assay kit from Siemens Healthcare (product code DF69A) that combines activities of lipoprotein lipase, glycerol kinase and glycerol-3-phosphate oxidase. Total cholesterol was measured using an enzymatic assay kit from Siemens Healthcare (product code DF27) that combines activities of cholesterol esterase and cholesterol oxidase. The assays for glucose, triglycerides and total cholesterol were automated on the Siemens Dimension EXL analyser. Free (non-esterified) fatty acids were measured using Roche’s Free Fatty Acid Kit (half-micro test) (Sigma Aldrich product code 11383175001) that is based on the enzymatic conversion of free fatty acids to acyl CoA by acyl-Co A synthetase. Leptin, adiponectin and resistin measurements were performed using enzyme-linked immunosorbent assay kits manufactured by MesoScale Discovery (MSD) Rockville, MD, USA, on a MSD s600 instrument, according to manufacturer instructions (K152BYC kit for leptin, K152BXC kit for adiponectin and K152FNC kit for resistin). All blood biochemistry measurements were performed at CBAL, Addenbrooke’s hospital.
Statistical analyses were performed using GraphPad Prism 8 software. For two groups, statistical analyses were performed using Mann–Whitney tests or un-paired Student’s t-tests with Welch’s correction (depending on the outcome of Shapiro–Wilk tests for normal distribution). Where more than two groups were analysed, we used one-way ANOVA, followed by Tukey’s multiple comparisons tests or two-way ANOVA followed by Sidak’s corrections for multiple testing, as appropriate. For growth kinetics analyses, we used mixed-effects model (REML) tests. For all tests, P values < 0.05 were considered significant. Detailed results of statistical analyses are shown in Supplementary Table 3.
Prentki, M. & Nolan, C. J. Islet beta cell failure in type 2 diabetes. J. Clin. Invest. 116, 1802–1812 (2006).
Sachdeva, M. M. & Stoffers, D. A. Minireview: Meeting the demand for insulin: molecular mechanisms of adaptive postnatal beta-cell mass expansion. Mol. Endocrinol. 23, 747–758 (2009).
Hudish, L. I., Reusch, J. E. & Sussel, L. β Cell dysfunction during progression of metabolic syndrome to type 2 diabetes. J. Clin. Invest. 129, 4001–4008 (2019).
Sandovici, I., Hammerle, C. M., Ozanne, S. E. & Constância, M. Developmental and environmental epigenetic programming of the endocrine pancreas: Consequences for type 2 diabetes. Cell. Mol. Life Sci. 70, 1575–1595 (2013).
Beaumont, R. N., Horikoshi, M., McCarthy, M. I. & Freathy, R. M. How can genetic studies help us to understand links between birth weight and type 2 diabetes?. Curr. Diab. Rep. 17, 22 (2017).
Horikoshi, M. et al. Genome-wide associations for birth weight and correlations with adult disease. Nature 538, 248–252 (2016).
DeChiara, T. M., Efstratiadis, A. & Robertson, E. J. A growth-deficiency phenotype in heterozygous mice carrying an insulin-like growth factor II gene disrupted by targeting. Nature 345, 78–80 (1990).
DeChiara, T. M., Robertson, E. J. & Efstratiadis, A. Parental imprinting of the mouse insulin-like growth factor II gene. Cell 64, 849–859 (1991).
Lui, J. C. & Baron, J. Evidence that Igf2 down-regulation in postnatal tissues and up-regulation in malignancies is driven by transcription factor E2f3. Proc. Natl. Acad. Sci. USA 110, 6181–6186 (2013).
Rotwein, P. The complex genetics of human insulin-like growth factor 2 are not reflected in public databases. J. Biol. Chem. 293, 4324–4333 (2018).
Estil’les, E., Téllez, N., Soler, J. & Montanya, E. High sensitivity of beta-cell replication to the inhibitory effects of interleukin-1beta: modulation by adenoviral overexpression of IGF2 in rat islets. J. Endocrinol. 203, 55–63 (2009).
Petrik, J., Arany, E., McDonald, T. J. & Hill, D. J. Apoptosis in the pancreatic islet cells of the neonatal rat is associated with a reduced expression of insulin-like growth factor II that may act as a survival factor. Endocrinology 139, 2994–3004 (1998).
Estil’les, E., Téllez, N., Escoriza, J. & Montanya, E. Increased β-cell replication and β-cell mass regeneration in syngeneically transplanted rat islets overexpressing insulin-like growth factor II. Cell Transpl. 21, 2119–2129 (2012).
Calderari, S. et al. Defective IGF2 and IGF1R protein production in embryonic pancreas precedes beta cell mass anomaly in the Goto-Kakizaki rat model of type 2 diabetes. Diabetologia 50, 1463–1471 (2007).
Petrik, J. et al. Overexpression of insulin-like growth factor-II in transgenic mice is associated with pancreatic islet cell hyperplasia. Endocrinology 140, 2353–2363 (1999).
Devedjian, J. C. et al. Transgenic mice overexpressing insulin-like growth factor-II in beta cells develop type 2 diabetes. J. Clin. Invest. 105, 731–740 (2000).
Modi, H. et al. Autocrine action of IGF2 regulates adult β-Cell mass and function. Diabetes 64, 4148–4157 (2015).
Hammerle, C. M. et al. Mesenchyme-derived IGF2 is a major paracrine regulator of pancreatic growth and function. PLoS Genet. 16, e1009069 (2020).
Bonner-Weir, S. Life and death of the pancreatic beta cells. Trends Endocrinol. Metab. 11, 375–378 (2000).
Rieck, S. & Kaestner, K. H. Expansion of beta-cell mass in response to pregnancy. Trends Endocrinol. Metab. 21, 151–158 (2010).
Lindström, P. Beta-cell function in obese-hyperglycemic mice [ob/ob Mice]. Adv. Exp. Med. Biol. 654, 463–477 (2010).
Buettner, R., Schölmerich, J. & Bollheimer, L. C. High-fat diets: modeling the metabolic disorders of human obesity in rodents. Obesity (Silver Spring) 15, 798–808 (2007).
Herrera, P. L. Adult insulin- and glucagon-producing cells differentiate from two independent cell lineages. Development 127, 2317–2322 (2000).
Srinivas, S. et al. Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. BMC Dev. Biol. 1, 4 (2001).
Wicksteed, B. et al. Conditional gene targeting in mouse pancreatic ß-Cells: analysis of ectopic Cre transgene expression in the brain. Diabetes 59, 3090–3098 (2010).
Qiu, W. L. et al. Deciphering pancreatic islet β cell and α cell maturation pathways and characteristic features at the single-cell level. Cell Metab. 25, 1194–1205 (2017).
Salic, A. & Mitchison, T. J. A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc. Natl. Acad. Sci. USA 105, 2415–2420 (2008).
Zavalza-Gómez, A. B., Anaya-Prado, R., Rincón-Sánchez, A. R. & Mora-Martínez, J. M. Adipokines and insulin resistance during pregnancy. Diabetes Res. Clin. Pract. 80, 8–15 (2008).
Medina-Gomez, G. et al. PPAR gamma 2 prevents lipotoxicity by controlling adipose tissue expandability and peripheral lipid metabolism. PLoS Genet. 3, e64 (2007).
Sandovici, I., Hoelle, K., Angiolini, E. & Constância, M. Placental adaptations to the maternal-fetal environment: implications for fetal growth and developmental programming. Reprod. Biomed. Online. 25, 68–89 (2012).
Napso, T., Yong, H. E. J., Lopez-Tello, J. & Sferruzzi-Perri, A. N. The role of placental hormones in mediating maternal adaptations to support pregnancy and lactation. Front. Physiol. 9, 1091 (2018).
Kahraman, S., Dirice, E., De Jesus, D. F., Hu, J. & Kulkarni, R. N. Maternal insulin resistance and transient hyperglycemia impact the metabolic and endocrine phenotypes of offspring. Am. J. Physiol. Endocrinol. Metab. 307, E906–E918 (2014).
Kervran, A., Guillaume, M. & Jost, A. The endocrine pancreas of the fetus from diabetic pregnant rat. Diabetologia 15, 387–393 (1978).
MacNeill, S., Dodds, L., Hamilton, D. C., Armson, B. A. & VandenHof, M. Rates and risk factors for recurrence of gestational diabetes. Diabetes Care 24, 659–662 (2001).
Russell, C., Dodds, L., Armson, B. A., Kephart, G. & Joseph, K. S. Diabetes mellitus following gestational diabetes: role of subsequent pregnancy. BJOG 115, 253–259 (2008).
Kim, J. K. Hyperinsulinemic-euglycemic clamp to assess insulin sensitivity in vivo. Methods Mol. Biol. 560, 221–238 (2009).
Schvartz, D. et al. Improved characterization of the insulin secretory granule proteomes. J. Proteomics 75, 4620–4631 (2012).
Pinheiro-Machado, E. et al. In silico approach to predict pancreatic β-cells classically secreted proteins. Biosci. Rep. 40, BSR20193708 (2020).
Xu, H. et al. Pancreatic β cell microRNA-26a alleviates type 2 diabetes by improving peripheral insulin sensitivity and preserving β cell function. PLoS Biol. 18, e3000603 (2020).
Small, L., Brandon, A. E., Turner, N. & Cooney, G. J. Modeling insulin resistance in rodents by alterations in diet: What have high-fat and high-calorie diets revealed?. Am. J. Physiol. Endocrinol. Metab. 314, E251–E265 (2018).
Cornu, M. et al. Glucagon-like peptide-1 protects beta-cells against apoptosis by increasing the activity of an IGF-2/IGF-1 receptor autocrine loop. Diabetes 58, 1816–1825 (2009).
Arous, C. et al. Integrin and autocrine IGF2 pathways control fasting insulin secretion in β-cells. J. Biol. Chem. 295, 16510–16528 (2020).
Fearnside, J. F. et al. Phylometabonomic patterns of adaptation to high fat diet feeding in inbred mice. PLoS ONE 3, e1668 (2008).
Duvillié, B. et al. Phenotypic alterations in insulin-deficient mutant mice. Proc. Natl. Acad. Sci. USA 94, 5137–5140 (1997).
Thorens, B. et al. Ins1(Cre) knock-in mice for beta cell-specific gene recombination. Diabetologia 58, 558–565 (2015).
Gannon, M., Kulkarni, R. N., Tse, H. M. & Mauvais-Jarvis, F. Sex differences underlying pancreatic islet biology and its dysfunction. Mol. Metab. 15, 82–91 (2018).
Tiano, J. P. & Mauvais-Jarvis, F. Importance of oestrogen receptors to preserve functional β-cell mass in diabetes. Nat. Rev. Endocrinol. 8, 342–351 (2012).
Takeo, C., Ikeda, K., Horie-Inoue, K. & Inoue, S. Identification of Igf 2, Igfbp2 and Enpp2 as estrogen-responsive genes in rat hippocampus. Endocr. J. 56, 113–120 (2009).
Faisal, M., Kim, H. & Kim, J. Sexual differences of imprinted genes’ expression levels. Gene 533, 434–438 (2014).
Pugliese, A. & Miceli, D. The insulin gene in diabetes. Diabetes Metab. Res. Rev. 18, 13–25 (2002).
Zeggini, E. et al. Replication of genome-wide association signals in UK samples reveals risk loci for type 2 diabetes. Science 316, 1336–1341 (2007).
Dai, N. et al. mTOR phosphorylates IMP2 to promote IGF2 mRNA translation by internal ribosomal entry. Genes Dev. 25, 1159–1172 (2011).
Mercader, J. M. et al. A loss-of-function splice acceptor variant in IGF2 is protective for type 2 diabetes. Diabetes 66, 2903–2914 (2017).
Kanatsuna, N. et al. Autoimmunity against INS-IGF2 protein expressed in human pancreatic islets. J. Biol. Chem. 288, 29013–29023 (2013).
Nica, A. C. et al. Cell-type, allelic, and genetic signatures in the human pancreatic beta cell transcriptome. Genome Res. 23, 1554–1562 (2013).
Dejeux, E. et al. Hypermethylation of the IGF2 differentially methylated region 2 is a specific event in insulinomas leading to loss-of-imprinting and overexpression. Endocr. Relat. Cancer 16, 939–952 (2009).
Brouwers, B. et al. Impaired islet function in commonly used transgenic mouse lines due to human growth hormone minigene expression. Cell Metab. 20, 979–990 (2014).
Kim, H., Kim, H., Kim, K., German, M. S. & Kim, H. Ectopic serotonin production in β-cell specific transgenic mice. Biochem. Biophys. Res. Commun. 495, 1986–1991 (2018).
Magnuson, M. A. & Osipovich, A. B. Pancreas-specific Cre driver lines and considerations for their prudent use. Cell Metab. 18, 9–20 (2013).
Mohan, R. et al. Differentially expressed MicroRNA-483 Confers distinct functions in pancreatic β- and α-cells. J. Biol. Chem. 290, 19955–19966 (2015).
Gunasekaran, U. & Gannon, M. Type 2 diabetes and the aging pancreatic beta cell. Aging (Albany NY) 3, 565–575 (2011).
Flurkey, K., Currer, J. M. & Harrison, D. E. Mouse models in aging research. In The mouse in biomedical research 2nd edn (eds Fox, J. G. et al.) 637–672 (Academic Press, 2007).
Mitchell, R. K. et al. Selective disruption of Tcf7l2 in the pancreatic β cell impairs secretory function and lowers β cell mass. Hum. Mol. Genet. 24, 1390–1399 (2015).
Rakshit, K., Hsu, T. W. & Matveyenko, A. V. Bmal1 is required for beta cell compensatory expansion, survival and metabolic adaptation to diet-induced obesity in mice. Diabetologia 59, 734–743 (2016).
Aoyagi, K. et al. VAMP7 regulates autophagy to maintain mitochondrial homeostasis and to control insulin secretion in pancreatic β-cells. Diabetes 65, 1648–1659 (2016).
Kilkenny, C., Browne, W. J., Cuthill, I. C., Emerson, M. & Altman, D. G. Improving bioscience research reporting: The ARRIVE guidelines for reporting animal research. PLoS Biol. 8, e1000412 (2010).
Zhang, Y. et al. Positional cloning of the mouse obese gene and its human homologue. Nature 372, 425–432 (1994).
Hirasawa, T., Ohara, T. & Makino, S. Genetic typing of the mouse ob mutation by PCR and restriction enzyme analysis. Exp. Anim. 46, 75–78 (1997).
Livak, K. J. & Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25, 402–408 (2001).
Halldorsdottir, S., Carmody, J., Boozer, C. N., Leduc, C. A. & Leibel, R. L. Reproducibility and accuracy of body composition assessments in mice by dual energy x-ray absorptiometry and time domain nuclear magnetic resonance. Int. J. Body Compos. Res. 7, 147–154 (2009).
This work was supported by Biotechnology and Biological Sciences Research Council (Grant BB/H003312/1 to M.C.); the Medical Research Council ([MRC_MC_UU_12012/4 and MRC_MC_UU_00014/4] to M.C, [MRC 979241] to C.H., [MRC_MC_UU_12012/5 and MC_UU_00014/5] to Metabolic Diseases Unit), the NIHR Cambridge BRC Cell Phenotyping Hub (in particular we wish to thank Natalia Savinykh for help with flow cytometry cell sorting) and the Spanish Ministry of Economy and Competitiveness (Grants BFU2012-33594 and BFU2013-47384-R to G. M.-G.). We thank Peter Voshol (Metabolic Diseases Unit) for advice regarding GTTs and ITTs; Claire Stocker and Kenneth Langlands (University of Buckingham) for sharing the protocol for β-cell mass measurement using pancreas stereology; Laura Dearden (Wellcome-MRC Institute of Metabolic Science) for help with hypothalamus collections at postnatal day 2; Adriana Jimenez and Roberta Jordan for technical help with the β-cell mass measurements; Adrian Wayman and Laura Hunter (West Forvie Phenomics Center) for help with mouse husbandry; Keli Philips and James Warner (Histopathology Core), for help with preparing tissue samples for histology; Gregory Strachan (Imaging Core facility) and Fatima Santos (The Babraham Institute, Cambridge, UK) for help with confocal microscopy imaging; Keith Burling (CBAL) for performing blood biochemistry measurements; Dan Hart (MRL Disease Model Core) for performing oral glucose tolerance tests for the pregnancy cohort.
The authors declare no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Sandovici, I., Hammerle, C.M., Virtue, S. et al. Autocrine IGF2 programmes β-cell plasticity under conditions of increased metabolic demand. Sci Rep 11, 7717 (2021). https://doi.org/10.1038/s41598-021-87292-x