Abstract
In oligotrophic waters, cnidarian hosts rely on symbiosis with their photosynthetic dinoflagellate partners (family Symbiodiniaceae) to obtain the nutrients they need to grow, reproduce and survive. For this symbiosis to persist, the host must regulate the growth and proliferation of its symbionts. One of the proposed regulatory mechanisms is arrest of the symbiont cell cycle in the G1 phase, though the cellular mechanisms involved remain unknown. Cell-cycle progression in eukaryotes is controlled by the conserved family of cyclin-dependent kinases (CDKs) and their partner cyclins. We identified CDKs and cyclins in different Symbiodiniaceae species and examined their relationship to homologs in other eukaryotes. Cyclin proteins related to eumetazoan cell-cycle-related cyclins A, B, D, G/I and Y, and transcriptional cyclin L, were identified in the Symbiodiniaceae, alongside several alveolate-specific cyclin A/B proteins, and proteins related to protist P/U-type cyclins and apicomplexan cyclins. The largest expansion of Symbiodiniaceae cyclins was in the P/U-type cyclin groups. Proteins related to eumetazoan cell-cycle-related CDKs (CDK1) were identified as well as transcription-related CDKs. The largest expansion of CDK groups was, however, in alveolate-specific groups which comprised 11 distinct CDK groups (CDKA-J) with CDKB being the most widely distributed CDK protein. As a result of its phylogenetic position, conservation across Symbiodiniaceae species, and the presence of the canonical CDK motif, CDKB emerged as a likely candidate for a Saccharomyces cerevisiae Cdc28/Pho85-like homolog in Symbiodiniaceae. Similar to cyclins, two CDK-groups found in Symbiodiniaceae species were solely associated with apicomplexan taxa. A comparison of Breviolum minutum CDK and cyclin gene expression between free-living and symbiotic states showed that several alveolate-specific CDKs and two P/U-type cyclins exhibited altered expression in hospite, suggesting that symbiosis influences the cell cycle of symbionts on a molecular level. These results highlight the divergence of Symbiodiniaceae cell-cycle proteins across species. These results have important implications for host control of the symbiont cell cycle in novel cnidarian–dinoflagellate symbioses.
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Introduction
Many cnidarians in the marine environment, including reef-building corals, form symbiotic relationships with photosynthetic dinoflagellates from the family Symbiodiniaceae1. These dinoflagellate symbionts are located in host gastrodermal cells inside symbiosomes (vacuoles consisting of a host-derived membrane)2. This closely integrated intracellular relationship indicates that symbiont population maintenance by the host was likely integral to the evolution of the symbiosis1,3. To date, most studies examining symbiont cell division in hospite have focused on nutrient availability4,5,6,7,8,9. However, symbiont growth rate appears to be controlled by more than nutrient limitation, as nutrient-replete symbionts in hospite still have a growth rate that is less than 20% of symbionts ex hospite5.
Besides nutrient control, other proposed host regulatory mechanisms of symbiont growth and proliferation include pre-mitotic cell-cycle control and post-mitotic autophagy, expulsion and apoptosis1,7,10,11. However, the contribution of each mechanism towards the regulation of symbiont biomass, from the onset to the stabilisation of the symbiosis, is unknown. Smith and Muscatine7 proposed that the main control of a dampened symbiont growth rate in hospite is from the cnidarian host arresting the cell cycle of its resident symbionts. In the eukaryotic cell cycle there is one quiescent stage (G0) and four subsequent cycling phases: G1 (gap 1) where cells grow and are sensitive to extracellular cues such as growth factors12; S (synthesis) where genomic DNA is replicated and synthesised13; G2 (gap 2), where DNA breaks that occur during the S phase are repaired before mitosis14; and M (mitosis), where two equal copies of the chromosomes are distributed between the two cells13. In the sea anemone Exaiptasia pallida (‘Aiptasia’), 80% of the resident symbionts were shown to be arrested at the G0/G1 phase compared with 40–55% in culture7.
Once a cell enters the cell cycle, it can be arrested at a series of cell-cycle checkpoints (Fig. 1). These checkpoints monitor the integrity and correct progression of the cell cycle with each checkpoint containing criteria that must be met for a cell to progress to the next stage of the cycle15,16. Each checkpoint is regulated by cyclin-dependent kinases (CDKs) and their partner cyclins17. Once a cell meets its checkpoint criteria, cyclins are synthesised and bind to their partner CDKs17. Cyclins regulate the catalytic activity of CDKs18. These CDK-cyclin complexes can directly trigger cell-cycle progression (Fig. 1) or indirectly trigger cell-cycle progression through a variety of other downstream events such as transcription, DNA damage repair, proteolytic degradation and metabolism19. Table S1 summarises the cell-cycle stage and roles of individual CDK and cyclin proteins. CDK-cyclin complexes in Homo sapiens are shown in Fig. 1; however, the type and quantity of CDKs and cyclins are specific to a particular species17.
Identification of cell-cycle proteins in the Symbiodiniaceae is just beginning, with a study by Cato et al.20 finding 10 distinct CDKs and 15 distinct cyclin genes in the genome of Breviolum minutum. In the same study20, qPCR analysis revealed that a cyclin B2/CDK1 pair was expressed during the G1/S phase transition in cultured B. minutum. As there are at least nine genera of Symbiodiniaceae21,22, determining whether cell-cycle proteins present in B. minutum are conserved across the Symbiodiniaceae will inform our understanding of cell-cycle progression and cellular growth rates in this family. For example, a recent study23 comparing cell-cycle progression between four Symbiodiniaceae genera (Symbiodinium, Breviolum, Cladocopium and Durusdinium) in culture, found that the proportion of the population progressing through the cell cycle was different between genera, resulting in differing growth rates. Similarly, different Symbiodiniaceae species have been shown to have different proliferation rates and reach different densities within the same host24,25,26,27, with inherent differences in cell-cycle machinery between species being one possible explanation. The current study represents the first attempt to identify and describe cell-cycle proteins across diverse Symbiodiniaceae species and provides a basis for future research.
Materials and methods
Identification of Symbiodiniaceae CDKs and cyclins
Twenty-seven Symbiodiniaceae transcriptome and genome databases were acquired from publicly available sources (Table S2). Cyclins and CDKs from two free-living Symbiodiniaceae species, Effrenium voratum28 and Fugacium kawagutii29, were compared with symbiotic species (Symbiodinium microadriaticum, S. tridacnidorum, Breviolum minutum, B. aenigmaticum, B. pseudominutum, B. psygmophilum, Cladocopium goreaui, Cladocopium genotypes C15 and C92 and Durusdinium trenchii). Profile hidden Markov models (pHMMs) were obtained from the PFAM 31.0 database for the cyclin N terminal domain (PF00134) and CDK conserved domain (PF00069). The pHMM models were re-trained using CDKs and cyclins from eukaryotic organisms closely related to Symbiodiniaceae (e.g. Apicomplexa) using the aphid R package30.
The Symbiodiniaceae databases were then queried with the updated pHMM models using an optimal alignment homology search to find putative cyclin and CDK sequences (Fig. S1). Sequences with log-odds similarity scores > 50 were retained for cyclins and CDKs. The cyclin model returned 119 sequences and the CDK model returned 6032 sequences. Due to the high abundance of Symbiodiniaceae CDK sequences returned from the model, the collected CDK sequences from the pHMM model were examined further using conserved CDK motifs (Table S3)31,32,33,34. If the CDK contained a motif that when BLASTp searched against the NCBI non-redundant database matched to a CDK, the sequence was retained for further analysis. All 119 cyclins retrieved by the model were also searched, and were included in the analysis if the highest-scoring sequence was annotated as a cyclin or CDK and had an E value ≤ 1 × 10−5. Owing to the lack of information available for CDKs and cyclins in other unicellular marine eukaryotes, several taxa (Table S5) were chosen for screening through the trained pHMM models to identify putative cyclin and CDK sequences, allowing possible alveolate-specific groups to be identified.
Sequence alignment and phylogenetic analysis
Phylogenetic trees were generated twice. The sequence alignment for the first set of trees was aligned to just the conserved cyclin N (PFAM ID:PF00134) and protein kinase domains (PFAM ID: PF00069), which were used to determine distinct phylogenetic groups of Symbiodiniaceae cyclins and CDKs. These were later used to identify other similar sequences from the Symbiodiniaceae databases.
The first trees were generated by aligning the putative CDKs and cyclins in the aphid R package30 (along with other eukaryotic cyclins and CDKs) and the best substitution model was selected by ProTest (v3.4)35. Both alignments had an appropriate evolutionary model of PROTOGAMMAAUTO, which was then used to infer maximum-likelihood trees in RAxML (v8.2.12)36. Bootstrap support was used to find the distinct phylogenetic groupings among Symbiodiniaceae CDKs and cyclins (n = 1000) by using the topology of the tree with the highest log-likelihood score. Trees were rooted using the H. sapiens MAPK (NP_002737.2) gene for the CDK tree and H. sapiens CABLES1 (NP_112492.2) and H. sapiens CABLES2 (NP_001094089.1) for the cyclin tree based on a previous study on animal cyclins and CDKs37. Symbiodiniaceae candidate proteins from distinct phylogenetic CDK and cyclin groups were used to perform custom BLASTp searches (Table S4) in Geneious v.11.1.5 (Biomatters Ltd.) against the 27 Symbiodiniaceae databases used in this study, to ensure that all putative CDKs and cyclins were identified. The first 10 Symbiodiniaceae proteins with the highest E-value (≤ 1 × 10−5) that were not previously identified by the pHMM model, and that identified a CDK or cyclin on the NCBI nr database in BLASTp searches, were collected from each Symbiodiniaceae database for each of the candidate proteins. These newly identified Symbiodiniaceae sequences were added to the previously collected sequences through the pHMM models and together these were entered into CD-Hit v4.838 to remove isoforms and redundant proteins using a similarity threshold of 90%.
Once redundant proteins and isoforms were removed, Symbiodiniaceae sequences were submitted to InterProScan39 to identify CDK and cyclin domains. Due to the low-quality annotations in Symbiodiniaceae databases40, many sequences contained regions that coded other proteins, therefore the alignments were trimmed manually in Geneious v.11.1.5 to CDK- (PFAM ID: PF00069; PANTHER ID: PTHR24056) and cyclin-(PFAM ID:PF00134, PF02984, PF16899 and PF08613; PANTHER ID: PTHR10177) annotated domains. The final CDK alignment for the second phylogenetic analysis was 465 amino acids (aa) long, and contained 177 Symbiodiniaceae sequences and 50 CDKs from other eukaryotes (Supplementary File S1), whereas the cyclin alignment was 395 aa long and contained 191 Symbiodiniaceae sequences and 54 cyclins from other eukaryotes (Supplementary File S2). All CDK and cyclin families from Homo sapiens were included in the trees to create the correct topologies, and CDKs and cyclins from other model organisms, including Saccharomyces cerevisiae and Arabidopsis thaliana, were only included if Symbiodiniaceae proteins were related to them.
Final CDK and cyclin alignments were run through ProTest (v3.4)35 as described previously. Maximum-likelihood trees were then run in PhyML (v3.1)41 using the Akaike information criterion, which corresponded to the LG + I + G + F model for the CDK alignment with a proportion of invariable sites of 0.039 and a gamma shape parameter of 1.195, and the LG + G + F model for cyclin alignments with a gamma shape parameter of 2.331. Due to the quantity of sequences in the tree, an approximate likelihood ratio test (aLRT) was used for branch support instead of bootstrap support42, however it has been shown to be very similar in calculating correct branch supports43. Based on a comparison of correct branch topologies determined by bootstrap support and SH-values43, true Symbiodiniaceae CDK and cyclin homologs were determined by branches containing an SH-value > 0.8. Trees were rooted as described previously. Trees were edited in the Interactive Tree of Life (iToL) software v.5.6.344. The nomenclature of protein groups that did not phylogenetically group with other well-classified CDKs or cyclins was attributed by using BLAST searches against the NCBI nr database.
Cyclin and CDK gene expression of Breviolum minutum
To explore expression of cyclins and CDKs in Symbiodiniaceae, RNA-Seq reads were analysed from a recent study by Maor‐Landaw et al.45 on the expression of cultured (n = 3) and freshly isolated Breviolum minutum (n = 3) from the sea anemone Exaiptasia diaphana (= pallida) (SRA PRJNA544863). Reads were aligned to the B. minutum genome assembly46 using STAR v2.7.1a in two-pass mode47 and read counts were extracted from the alignments with featureCounts v1.6.348. Differential expression analysis was completed using the exact test in EdgeR49 on TMM normalized counts of the cultured and isolated B. minutum. Differentially expressed genes (DEGs) were those with Benjamini–Hochberg adjusted p-values < 0.05. Cyclins and CDKs identified in B. minutum were selected from the list of DEGs to generate a heat map in the R environment50, using the mean–variance modelling at the observational level (voom)51 of log2-transformed counts per million (CPM).
Results and discussion
Characterisation and phylogenetic positioning of Symbiodiniaceae CDK sequences
Eukaryotic organisms contain different numbers of CDK proteins, ranging from three in premetazoans, to 20 in eumetazoans such as Homo sapiens37. A total of 177 unique Symbiodiniaceae CDK gene copies were identified across six genera (Table 1). CDK gene copy numbers were the highest in Cladocopium goreaui, which contained 16 CDK copies. Interestingly, no CDKs related to the CDK4/6 family nor their cyclin partners (cyclin E) were found in Symbiodiniaceae using the databases referenced in this study (Table 1; Fig. 2). This agrees with findings for plants and many protists, in which there is also an absence of the CDK4/6 family and cyclin E in most pre-metazoan lineages37.
Some of the Symbiodiniaceae CDKs showed high sequence similarity to eumetazoan CDKs, however the largest expansion of CDKs was within the alveolate-specific CDK groups (Table 1, Fig. 2). A previous study20 investigating Symbiodiniaceae cell-cycle proteins found four B. minutum-specific CDKs. Here we show that three of those four CDKs are also present across other Symbiodiniaceae species (alveolate-specific CDKG/H/J—Table 1; Supplementary Fig. S2). In the previous study20, the B. minutum CDKs (alveolate-specific CDKG/H/J) did not change their expression with cell-cycle phase when in a free-living state. However, our analysis of the previously published RNA-Seq data45 shows that symbiosis alters the expression of B. minutum CDKG and CDKH, which were both up-regulated in hospite compared to when in culture (Table S6; Fig. 3).
The most common CDK identified in Symbiodiniaceae was an alveolate-specific CDK (CDKB) with gene copies found across 18 species in the five Symbiodiniaceae genera examined (Table 1). Symbiodiniaceae proteins in the CDKB group contained the canonical CDK motif PSTAIRE (Table 2). The CDKB sister clade is the Pho85/CDK5 subfamily (SH-value 0.95), which is sister to the metazoan CDK1/S. cerevisae Cdc28, with strong branch support (SH-value = 1; Supplementary Fig. S2). CDK1/Cdc28 is the primary cell-cycle regulator from yeast to humans52,53,54, however Pho85 has been shown to have overlapping roles with Cdc28, phosphorylating many of the same substrates55. The primary roles of Pho85 include responding to environmental cues via the induction of signals that inform the cell whether conditions are adequate for cell division and nutrient metabolism 56. As Symbiodiniaceae proliferate in response to increased nutrients5, they may have evolved CDKs that possess similar functions for linking external stimuli (e.g. environmental nitrogen and phosphorus levels) to cell-cycle progression. Furthermore, our analysis of the RNA-Seq data comparing cultured versus freshly-isolated B. minutum45 suggests that two CDKB genes are up-regulated in symbiosis (Table S6; Fig. 3). We hypothesise that, due to its phylogenetic grouping, conserved motif, widespread presence across Symbiodiniaceae and up-regulation in the symbiotic state, CDKB may be a homolog of Cdc28/Pho85 and a primary cell-cycle regulator in Symbiodiniaceae. This hypothesis requires confirmation.
Proteins related to eumetazoan transcriptional CDK subfamilies (CDK9/12/13 (SH-value = 0.89), CDK10/11 (SH-value = 0.89) and CDK20 (SH-value = 0.93)) were also present in Symbiodiniaceae (Table 1; Supplementary Fig. S2). Amongst transcriptional roles, the CDK10/11 subfamily has also been proposed to have roles in cell-cycle progression during the G2/M phase (Table S1)57. However, in B. minutum, CDK20, CDK9 and CDK11 expression did not change with cell-cycle phase20, highlighting their similarity to metazoan CDK20, CDK9 and CDK11, which are predominantly transcriptional CDKs and indirectly related to the cell cycle58. Previous studies20 have reported an absence of CDK7 in B. minutum, however this study found a CDK7-related gene (confirmed via BLAST searches on the NCBI nr database) across 13 different Symbiodiniaceae species (Supplementary Fig. S2). The difference in results may be explained, in part, by the Symbiodiniaceae CDK7 being phylogenetically distant from the metazoan CDK7 and yeast CDK7 homolog (Kin28p), grouping separately and with no concrete relationship to any other CDK included in this study, possibly owing to its divergence. CDK7 has been discovered in other basal organisms, such as the amoebozoan Dictyostelium purpureum59. In metazoans, CDK7 forms part of the cyclin kinase-activating (CAK) complex that activates other CDKs by phosphorylating their T-loop60, and inhibition of CDK7 led to the arrest of the cell cycle in proliferating cells61. The previously published RNA-Seq data45 show that the CDK7-related gene was up-regulated in symbiotic B. minutum (Table S6).
Symbiodinium sp. #2 contained CDKs and cyclins that are more similar to those of the free-living dinoflagellate Amphidinium (SH-value > 0.95) than other Symbiodiniaceae species (Supplementary Fig. S2). CDKs and cyclins that are not present in Amphidinium sp. but are present in Symbiodinium sp. #2 grouped next to, not with, the other Symbiodiniaceae species (SH-value > 0.78). This placement may reflect the basal status of Symbiodinium within the Symbiodiniaceae21.
Several Symbiodiniaceae species contained CDKs found in parasitic taxa. A CDK protein that is related to a gene present in the free-living, facultative pathogenic marine ciliate Pseudocohnilembus persalinus, was found in both D. trenchii and Cladocopium sp. #1 (SH-value = 1), while C. goreaui harbours a CDK related to Cdc2-related kinase 6 (CRK6) from Trypanosoma brucei (SH-value = 0.97) (Fig. 2, Supplementary Fig. S2). Studies62,63 have shown that the loss of T. brucei CRK6 slows cell growth but does not inhibit the cell cycle (contrasting with cell cycle indispensable CRK3 and CRK1), highlighting a function of CRK6 that may not be directly associated with the cell cycle.
Characterisation and phylogenetic positioning of Symbiodiniaceae cyclin sequences
Similar to CDKs, the number of cyclins differs across eukaryotes – from eight in premetazoans to 29 in Homo sapiens37. Across the six Symbiodiniaceae genera examined, 191 cyclins were identified (Table 3; Fig. 4). C. goreaui contained the most cyclin gene copies, harbouring 19 distinct copies. Differences in abundance of cell-cycle proteins (cyclins and CDKs) between different Symbiodiniaceae species could be a result of the different database information provided (genomes versus transcriptomes), as if CDKs and cyclins were not expressed at the time of transcriptomic analysis, these may have been missed, thus producing a bias towards genomes harbouring more cyclin and CDK gene copies. Another possible reason for the difference in cyclin and CDK gene copies in the Symbiodiniaceae are gene duplication events, which are followed by genetic drift over time, causing the formation of cell-cycle paralogs with functional divergence in the family.
All the cyclins found in the Symbiodiniaceae contained one of three distinct domains (Fig. 5): the conventional cell-cycle cyclin N and C domains; a cyclin N domain found nearer the amino terminus than the position of the conventional cell-cycle cyclin N domain which corresponded phylogenetically to transcriptional cyclins (specifically cyclin L); and a single plant P/U cyclin domain that is phylogenetically related to the analogous domain of the Pho80p cyclin in S. cerevisiae.
Proteins related to eukaryotic cell-cycle cyclins A, B, D and G/I, and transcriptional cyclin L were identified in the Symbiodiniaceae, along with proteins related to plant cyclin D, protist/plant P/U-type cyclin and cyclin Y, as well as genes related to Cyc2 and mitotic Cyc6 from the sister taxon Apicomplexa (Fig. 4; Supplementary Fig. S3). Three phylogenetically distinct groups of cyclins were also present in Symbiodiniaceae, that upon searching the NCBI nr database, matched to alveolate-specific cyclins A/B (Supplementary Fig. S3). Two cyclins previously reported to be B. minutum-specific20 were found in other Symbiodiniaceae species and belong to the “Plant Cyclin D-like” grouping (Table 3; Supplementary Fig. S3). In metazoans and plants, cyclin D is required for G1 phase progression64.
An expansion of the protist/plant P/U-type cyclin groups was found within Symbiodiniaceae, with 63 gene copies being present across six Symbiodiniaceae genera (Table 3, Fig. 4, Supplementary Fig. S3). This finding agrees with the previous study20, which found P-type cyclins in B. minutum. Genes within these groups were related to the S. cerevisiae Pho80p cyclin. In S. cerevisiae, the Pho80 subfamily of P/U-type cyclins (Pho80, Pcl6, Pcl7, Pcl8 and Pcl1055) links nutrient availability with cell-cycle progression65. In A. thaliana, P/U-type cyclins are implicated in the switch from heterotrophic to autotrophic growth66. RNA-Seq data45 revealed that two of these P/U type cyclins had contrasting expression (one being up-regulated whilst the other was down-regulated) in hospite versus in culture in B. minutum (Table S6; Fig. 3). Given that nutritional exchange is a fundamental feature of the cnidarian–dinoflagellate symbiosis1, and that P/U cyclins are involved in glycogen metabolism and carbon source utilisation56,67, the differential expression of these cyclins in hospite is unsurprising. Whether the difference in expression is a response to environmental stimuli exclusively experienced in symbiosis, e.g. host-associated factors such as the pH of the symbiosome in which the alga resides68, requires further study. Similar to Symbiodiniaceae, the apicomplexan T. gondii also lacks a cyclin E homolog and instead uses a P-type cyclin for G1 phase progression69. Symbiodiniaceae may also use P-type cyclins in place of eumetazoan cyclin E, however this requires confirmation.
Twenty two cyclin Y-like gene copies were found across the Symbiodiniaceae. These encompassed two phylogenetic groups, one termed “Cyclin Y” which grouped with eumetazoan Cyclin Y (SH-value = 0.93), and one group of cyclins that grouped as a sister group with the conventional eumetazoan Cyclin Y (SH-value = 0.80) that were termed “Cyclin Y-like” (Fig. 4, Supplementary Fig. S3). Cyclin Y is absent in plants and fungi (being replaced by the Pcl class of cyclins in fungi) but is present in animals and protists59. In eumetazoans and fungi, cyclin Y and Pcl1 cyclins are the binding partners of CDK14 and Pho85, respectively70,71. In yeast, the cyclin Y homolog, Pcl1, is expressed during the G1 phase of the cell cycle70 and provides information to the cell, determining whether it passes the START checkpoint, where the yeast cell commits to mitosis56. In Drosophila, cyclin Y is required for Wnt signalling by localising the CDK14 kinase to the cell membrane72. As Wnt signalling is an indispensable pathway for the long-term viability of cells73, the presence of cyclin Y and cyclin-Y like genes in most eukaryotes is predicted.
Uniquely, C. goreaui and D. trenchii both contain cyclins present in two phylogenetic groups that cluster with mitotic cyclins from the dinoflagellate sister taxon, the apicomplexans74 (Fig. 4). One group is related to the B-type G2/M phase-specific cyclin, Cyc6, in the apicomplexans (SH-value > 0.98), while the other clusters with Cyc2-like from T. brucei, which is involved in transition from both the G1 to S and G2 to M phases75 (Fig. 4). The correlation in cell-cycle machinery of both cyclins and CDKs between pathogenic protists and D. trenchii, which is reported to colonise hosts during heat stress opportunistically25,76 and has a fast growth rate versus other Symbiodiniaceae species in culture23, is noteworthy and warrants future investigation.
Cladocopium sp. C15 harbours two cyclins (cyclin D and cyclin G/I) that are related to those in the symbiotic coral, Stylophora pistillata, with strong support (SH-value = 1). Both Cladocopium sp. C15 cyclin D and G/I share a similar identity (92.1% and 74.5%, respectively) and similarity (95.7% and 91.6%, respectively), across the full sequence length to S. pistillata cyclins. To account for possible contamination of host material in the Cladocopium sp. C15 transcriptome, the origin of this symbiont was traced77. The Cladocopium sp. C15 was found to have been freshly isolated from its host Porites compressa, so host contamination cannot be excluded. This being said, symbiosis has been suggested to drive the formation of paralogous genes involved in host-symbiont interactions due to selective pressure for a more mutualistic partnership between host and symbiont78. How the evolution of cell-cycle proteins that share a high similarity between host and symbiont affects biomass co-ordination is deserving of future attention.
Conclusions
Our study shows the divergence of cell-cycle proteins in the Symbiodiniaceae family and demonstrates that there are several conserved CDK and cyclin groups across the Symbiodiniaceae, though also marked species-specific differences. Which of these conserved cell-cycle proteins are indispensable for cell-cycle progression and which species-specific proteins influence proliferation rates in symbiosis remains unknown. Further study will be required to clarify which CDKs and cyclins are required for Symbiodiniaceae cell-cycle progression, and whether this differs between species and symbiotic states. As annotation of Symbiodiniaceae genomes is challenging79, future studies should aim to apply the same comparative analysis across new Symbiodiniaceae genomes to inform cyclin and CDK gene prediction accurately.
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Acknowledgements
This research was supported by the Marsden Fund of the Royal Society Te Apārangi of New Zealand (Grant Number VUW1601), awarded to S.K.D., A.R.G., V.M.W. and C.A.O. and an associated PhD scholarship to L.M.G. Additional support was provided via strategic research funds from the School of Biological Sciences at Victoria University of Wellington to S.K.D.
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The study was conceptualised by L.M.G., C.A.O., A.R.G., V.M.W., and S.K.D. L.M.G. wrote the manuscript, collected and analysed the sequence and phylogenetic data, and constructed all figures (apart from figure 3) and all tables. S.P.W. helped L.M.G. with data collection and analysis. S.A.K. conducted and analysed the expression analysis, constructed figure 3, helped revise the manuscript, and advised on methods, data analysis and visualisation, C.A.O., V.M.W., S.K.D., A.R.G. helped edit and refine the manuscript. All authors reviewed the manuscript before submission.
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Gorman, L.M., Wilkinson, S.P., Kitchen, S.A. et al. Phylogenetic analysis of cell-cycle regulatory proteins within the Symbiodiniaceae. Sci Rep 10, 20473 (2020). https://doi.org/10.1038/s41598-020-76621-1
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DOI: https://doi.org/10.1038/s41598-020-76621-1
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