Abstract
A rapid method for screening pathogens can revolutionize health care by enabling infection control through medication before symptom. Here we report on label-free single-cell identifications of clinically-important pathogenic bacteria by using a polymer-integrated low thickness-to-diameter aspect ratio pore and machine learning-driven resistive pulse analyses. A high-spatiotemporal resolution of this electrical sensor enabled to observe galvanotactic response intrinsic to the microbes during their translocation. We demonstrated discrimination of the cellular motility via signal pattern classifications in a high-dimensional feature space. As the detection-to-decision can be completed within milliseconds, the present technique may be used for real-time screening of pathogenic bacteria for environmental and medical applications.
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Introduction
Bacteria constitute a major class of pathogens in environments including water and food1,2,3. They are evolved in distinct ways to spontaneously move by using motile filamentous organelle protruding from the micrometer-scale bodies for invading in organisms and multiply themselves. While many of them are innocuous, there are also toxic strains that may bring detrimental impacts on health of individuals to global economy through causing infection disease outbreaks. Plate counting and polymerase chain reaction-based genomic analyses have thus been used as effective means to inspect presence or absence of bacteria in biosamples. However, the methods in general involve many pretreatment steps and thus time-consuming often requiring hours for the tests to be completed4,5, despite that the risk of infection outspread rises quickly in general as the microbes grow in number in the hosts. It is therefore of urgent importance to construct a reliable and highly-sensitive sensor for rapid analyses of viable microbes at an early stage of infection.
Here we report on use of solid-state micropores for label-free single-cell discriminations of five clinically-important pathogenic bacteria, Staphylococcus aureus, Pseudomonas fluorescens, Salmonella enterica, Escherichia coli and Bacillus cereus6,7,8,9, by their unique motility. The working mechanism is based on single-cell detections via Coulter principle that measures transient drops in the cross-membrane ionic current upon translocation of microbes through the conduit10. Unlike the conventional Coulter counters having relatively high thickness-to-diameter aspect ratio channel structures, a special care was taken in the present study to exploit it for detecting bacterial motility by employing a low aspect ratio pore architecture11,12,13 to make the ionic current more sensitive to multiple physical parameters of analytes such as shape14,15, surface charge density16, mass17,18, surface proteins19,20, and even the translocation motions21. Furthermore, since the enhanced sensor sensitivity is expected to yield more complicated ionic current signal patterns, we employed machine learning to pattern-analyze bacteria-derived fine features in the electrical signatures so as to compare and discern the multitude of physical properties of individual microbes in a high dimensional feature space.
Results
The micropore consists of a lithographically-defined hole of diameter dpore formed in a 50 nm thick SiNx membrane (Fig. 1a,b). In experiments, bacteria dispersed in phosphate buffer saline (PBS) was added to one side of the membrane while the other side was filled with only PBS. Two Ag/AgCl rods were then used as electrodes to measure the ionic current Iion flowing through the microscale conduit under the applied dc voltage Vb. Backside of the substrate was coated with a micrometer-thick PMMA layer (Fig. 1c) to reduce the device capacitance for lowering high-frequency current noise22 as well as to ensure fast temporal resolution of the sensor23 for detecting rapid change in Iion during the single-cell translocation.
The ionic current through the SiNx pore of diameter dpore = 3 μm was measured to be around 24 nA at Vb = 0.05 V (Fig. 1d). This manifests the predominant influence of the access resistance24 Racc = ρ/dpore = 2 MΩ on Iion compared to the component Rpore = 4ρL/πdpore2 = 42 kΩ inside the channel, where L = 50 nm is the channel depth and ρ = 6 Ωm is the resistivity of the electrolyte buffer. Meanwhile, each time when a bacterium passed through the pore, it temporarily blocked the ion flow thereby caused a pulse-like Iion drop (Fig. 1e).
The Iion signatures are anticipated to reflect the intrinsic physical properties of the bacteria. To see if there is any difference in the resistive pulse profiles among the five species, therefore, we constructed scatter plots of the signal height Ip as a function of the width td that are known to represent the characteristic size and the time required for the cell to pass through the micropore, respectively (Fig. 2). As a result, we found significant overlap in their distributions, naturally interpreted as a consequence of their resembling micrometer-scale morphologies as confirmed by electron microscope observations (Table 1).
Under this circumstance, it becomes important to explore other features in the Iion signatures that can better distinguish the pathogenic bacteria. For this, we first investigated the physical origins of the variations in the resistive pulse patterns by performing finite element simulations13 of the cross-membrane ionic current. We built three-dimensional models of the bacteria deduced from their morphologies depicted in the scanning electron micrographs. By changing the position z of the modeled structure along the axial direction, we obtained Iion blockade characteristics for the five microbes (Fig. 3a), where we tentatively assumed an up-right orientation of the cell with respect to the membrane surface. The theoretical resistive pulses were then compared to the Iion signals recorded in the experiments (Fig. 3b, see also Fig. S1). Here, we normalized the ionic current by the signal height for the quasi-spherical S. aureus Iaur. The results revealed underestimations of the resistive pulse heights in the finite element analysis for the non-spherical microbes (Fig. 3c). This can be ascribed to tilted bacterial conformations during the actual translocation in the experiments that differ from the vertical configurations assumed in the simulations. Indeed, the model calculations revealed a non-linear increase in the pulse height with the bacterial tilting angle θ for all of the five species (Fig. 3d), whose effect is also found to be dependent on the microbial aspect-ratio motifs demonstrating a factor (αrot) of 1.1 for the quasi-spherical S. aureus while larger than 2 in cases of the other rod-shaped bacteria.
In this regard, it is noticeable that bacterial motility known as galvanotaxis would induce tilting motions under the focused in-pore electric field, wherein beating kinetics of cilia generates torque to rotate the rod-like cell body in response to the electrical stimulli25,26. To verify its possible role on the translocation dynamics, we extended the resistive pulse measurements to different Vb conditions (Fig. 3e, see also Figs. S2-S3). A control test (Fig. S4) on 1.1 μm-sized carboxylated polystyrene microbeads showed almost constant Gp = Ip/Vb suggesting linear increase in the ionic current with the applied voltage following the ohm’s law. In sharp contrast, E. coli yielded a steady increase in Gp with Vb. The result implies a higher chance of having more tilted rod-shaped body in the pore under the larger electric field via the galvanotaxis-derived faster rotation motions (Fig. 3e).
Taking the electric field-derived ciliary motions of microbes into account, we deduced their most-likely tilting angle in the micropore. When assuming that Idif stems solely from the varying orientations of the cell bodies (Fig. 3c), the ratio Iexp/Itheo can be regarded as equal to the deviation αrot (Fig. 3e). From this, we roughly estimated θ that indicated prominent roles of the unique motility to render bacteria-specific conformations with E. coli being the most oblique one (Fig. 3f).
Besides the rotation, the galvanotactic response is known to add a momentum to move the negatively-charged bacterium in direction opposite to the electrophoresis25,26. Indeed, plots of the resistive pulse width td against Idif elucidated longer translocation time for the microbes exhibiting larger contributions of galvanotaxis (Fig. 4a), which can be interpreted as a consequence of the slower electrophoretic speed under the stronger back-force (Fig. 4b,c). E. coli is an exception displaying relatively short td that would be due to the shorter effective distance the large-θ bacterium travels within td. The overall findings consistently indicated the prominent role of galvanotaxis on the Iion signal profiles.
We now quantify the discriminability of the five bacteria by the resistive pulse shapes. Usually, the single-cell analysis is implemented by looking at the signal heights10,27 that allows statistical discriminations of bacterial species as far as there is clear difference in the cell size28,29,30,31. On the other hand, the above results elucidated the peculiar sensitivity of the ionic current blockage in the low-aspect-ratio micropores on non-volume properties including the bacterial shape and motility. This calls for an analytical method that enables to leverage not only the pulse height but also finer features for distinguishing the ionic current signals. Therefore, we herein employed a classifier ensemble approach of Rotation Forest32 in WEKA33 to interrogate the resistive pulse patterns in a high-dimensional feature space. Specifically, we meshed the Iion—time domain into dh × dw regions (Fig. 5a) and counted the number of plots in each section (Fig. 5b). We also extracted researcher-crafted parameters from the signals such as the pulse height Ip, width td, bluntness β, onset angle θon, area A, pulse asymmetry rm, and inertia Ilat and Iver as summarized in Fig. S5. All of these were randomly selected to create 60 feature vectors and utilized for training 67 Rotation Forest ensembles (Fig. 5c,d). The algorithm then carried out the first classification for the resistive pulse data sets of known bacteria. Afterward, it internally exhibited a principal component analysis to choose the effective parameters with respect to the pretest scores, i.e. the number of correct outputs for judging labelled input signals, and subsequently used them to newly generate feature vectors for the post single-pulse discriminations (Fig. 5e). In this way, one can simultaneously use the multiple features on the same footing for classifying the varying pulse patterns reflecting the stochastic translocation motions of the motile bacteria.
The actual analysis was exhibited for randomly-chosen 87 resistive pulses. Among the signals, 79 were used for training classifiers to testify the discriminability of the bacteria by actually implementing resistive pulse classification for the rest of 8 pulses (tenfold cross validation). From the output of the algorithm on each labelled signals, we estimated the rate of true-negative, true-positive, false-negative, and false-positive cases. The result is summarized in a form of confusion matrix (Fig. 5f) that illustrates the high discriminability of the five bacteria with F-measure score Fmeas reaching 0.91, which suggests 91% accuracy for identifying the bacteria from the Iion signature profiles at a single-cell level. This discriminability can be judged as good considering the worst case be Fmeas = 0.2 by a random guess of the five species.
It is of interest to know what enabled the single-bacteria discriminations. Nonetheless, it is not possible to figure out which of the researcher-defined parameters played an important role on the classifications due to the non-physical meaning of the internally-created feature vectors in the ensemble learning algorithm. Yet, we could at least confirm that the results were not affected by factors other than physical features of the bacterial cells. For instance, the bacterial concentration in the suspension was found to cause no notable influence on the signal waveforms (Fig. S6). This is ascribed to the principle of the ionic current blockage that reflects only the physical properties of objects passing through the sensing zone. Moreover, dead cells were observed to be not able to transit the pore (Fig. S7), and hence they were not detected, due presumably to the denatured surface proteins with less charges that render inadequate electrophoretic forces to draw the cells captured into the channel. In fact, the present study used fresh bacterial samples by cultivating on the day of the resistive pulse measurements to minimize the population of dead cells. Although this does not rule out the possible translocation of cell fragments during the experiments, their small sizes are naturally anticipated to cause ionic current drops that are too small to be detected under the given noise floor (Fig. S8).
While the training process is laborious and time-consuming due in part to the involved pulse extractions from the Iion-t data recorded at the 1 MHz sampling rate, the actual single-pulse classification per se requires less than a millisecond with a standard CPU. The machine-learning-driven resistive pulse analysis can therefore be a real-time method for label-free identifications of single-bacteria. The sensor concept is also viable to virtually any bacteria as long as they can be put through the sensing zone, the capability of which may find various bioanalytical applications let alone the case sensitive pretreatments required to ensure no inclusion of oversized objects in samples that may potentially clog the channel.
Conclusions
Single-cell identifications of clinically important pathogenic bacteria were examined by a machine learning-driven resistive pulse analysis using a low aspect-ratio micropore. The shallow channel structure rendered a particular sensitivity of the blockade ionic current to the three-dimensional translocation motions of the microbes that enabled to find distinct signatures of their unique motility under the imposed electric field in the signal profiles. In order to compare and discern the bacteria-specific nontrivial features in the resistive pulses, we exhibited data classification based on an ensemble-learning algorithm in a high-dimensional feature space, which was proven useful to detect and identify the physically resembling five bacteria in real time at the single-cell level.
Methods
Preparation of bacteria
Following bacteria were used as model microorganisms for micropore analysis. Staphylococcus aureus (No. 13276, NITE Biological Resource Center, Japan), Pseudomonas fluorescens (isolated strain from food sample donated from Meiji Co. Ltd., Japan), Salmonella enterica (JCM 1652, RIKEN BioResource Center, Japan), Escherichia coli (No. 3972, NITE Biological Resource Center, Japan) and Bacillus cereus (JCM 2152, RIKEN BioResource Center, Japan). E. coli, B. cereus, S. aureus were cultivated aerobically in Trypticase Soy Broth (pH 7.3) containing 1.7% Bacto tryptone, 0.3% Bacto Soytone, 0.25% Glucose, 0.5% Sodium chloride, 0.25% Dipotassium hydrogen phosphate in ultrapure water. S. enterica and P. fluorescens were cultivated aerobically in Nutrient Broth (pH 7.0) containing 0.5% Bacto peptone, 0.3% Difco Beef extract and 0.5% Sodium chloride in ultrapure water. E. coli, S. aureus and S. enterica were cultured at 37˚C while B. cereus and P. fluorescens were cultured at 30 °C for 4 h after preculture under same conditions. After culture, cells were centrifuged at 1000 g for 10 min, washed twice and resuspended in 0.1 × PBS, phosphate buffered saline containing 0.08% sodium chloride, 0.002% potassium chloride, 0.012% sodium monohydrogen phosphate, and 0.02% potassium dihydrogen phosphate at pH 7.4.
Fabrication of low-aspect-ratio micropores
A Si wafer diced into 30 mm square chips was used as a substrate. On the both sides of the Si layers, there were 50 nm thick SiNx formed by low-pressure chemical vapor deposition. The bottom side of the SiNx was partially removed by reactive ion etching through a metal mask having a square window of 1 mm × 1 mm size. The exposed Si was immersed in KOH aq. and heated to 80 degrees Celsius for wet etching, which led to formation of a SiNx membrane at the other side of the chip. On the membrane, electron beam resist was spin-coated and baked at 180 degrees Celsius. A circle of 3 μm diameter was delineated by electron beam lithography. After development, the residual resist was used as a mask to open the micropore by isotropic reactive ion etching of the exposed SiNx.
Back-side polymer coating
On the back-side of the micropore chip, we spin-coated a polymethyl methacrylate (PMMA) layer of about 5 μm thickness. After baking, we irradiated electron beam from the front side of the surface at 50 μm around the micropore followed by development to remove the PMMA in the micropore.
Micropore sealing
Two blocks made of polydimethylsiloxane (PDMS) were used to seal the micropore. These blocks were made by curing PDMS on a Si substrate with an SU-8 layer patterned to have a straight motif of 15 mm length and 100 μm thickness. After polymerization in oven at 80 degrees Celsius, the blocks were cut out with knife. Because of the SU-8 pattern, there was a fluidic channel formed on the bottom surface of the PDMS block. In prior to the sealing, the channel side of the PDMS block as well as the micropore chip was exposed to oxygen plasma for surface activation. Subsequently, the two surfaces were attached together for eternal bonding. This process was repeated again for another PDMS block to seal the other side of the micropore.
Ionic current measurements
Through holes punched in the PDMS block, we injected PBS containing bacteria at one side of the micropore and bacteria-free buffer at the other side. Ag/AgCl rods were then placed at the both sides of the micropore. The ionic current through the micropore was measured by applying dc voltage between the Ag/AgCl and recording the output current through a custom-built preamplifier backed by a fast digitizer at a 1 MHz sampling rate.
Finite element analysis
The ionic current through the micropore was simulated by a finite element method. A three-dimensional model was constructed, which was composed of a cylindrical cell of 50 μm diameter and 100 μm height with a 50 nm thick SiNx disk at the middle. A 3 μm-sized micropore was opened in the disk and the cell was filled with water containing Na+ and Cl- ions at a concentration of 13.7 mM. The electric potential at one end was set to 0 V while the other side to + 0.05 V. The ionic current was calculated by solving Poisson-Nernst-Plank and Navier–Stokes equations in a self-consistent manner using COMSOL Multiphysics 5.4.
Machine learning-driven resistive pulse pattern analysis
Classification of resistive pulse patterns was performed using the machine learning workbench WEKA with Rotation Forest ensembles each employing a distinct base classifier. Researcher-crafted feature parameters were defined and exploited in a machine learning-based classification of resistive pulses. These are: the height Ip; the width td; the bluntness at the pulse apex βapex; the onset angle θ, the pulse peak position r; the area A; the ratio rm between the area before and after the peak maximum; the inertia Im and Iw calculated with respect to longitudinal and transverse axes, respectively (detailed definitions of these parameters are explained in Fig. S5). The feature parameters were obtained from each resistive pulse data. Each parameter was coupled to the current vector and the time vector to create feature vectors, which were then used for training classifiers. 90% of the 87 resistive pulses of known bacteria were used for the training, and the trained classifiers judged the other 10%. The average accuracy of the bacterial discrimination was assessed by repeating this procedure ten times through interchangeably changing the teacher data and the test data (tenfold cross validation). The F-measure score Fmeas = 2PPrePRec/(PPre + PRec) was deduced as an average of the whole combinations of the classifiers and the feature vectors, where PPre and PRec are the precision and recall calculated through TP/(TP + FP) and TP/(TP + FN), respectively, with TP, FP, and FN being the number of true-positive, false-positive, false-negative cases, respectively, estimated from the output for the labelled resistive pulse data.
Data availability
All data and material are available for readers in methods and supplementary information.
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Acknowledgements
A part of this work was supported by ImPACT Program of Council for Science, Technology, and Innovation (Cabinet Office, Government of Japan), the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number 19K22108 and 18H01795, and The Food Science Institute Foundation, Ryoushokukenkyukai (Tokyo, Japan).
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T.W., T.K., and M.O. planned the research. L.I.W. and M.Tsutsui fabricated micropores. S.H., R.S., M. Tsutsui, A.A., M.Tanaka, and K.Y. performed measurements and analyzed data. M.Tsutsui, T.W., T.K., and M.O. co-wrote the paper. All authors reviewed the manuscript.
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Hattori, S., Sekido, R., Leong, I.W. et al. Machine learning-driven electronic identifications of single pathogenic bacteria. Sci Rep 10, 15525 (2020). https://doi.org/10.1038/s41598-020-72508-3
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DOI: https://doi.org/10.1038/s41598-020-72508-3
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