Global viticulture has evolved following market trends, causing loss of cultivar diversity and traditional practices. In Montenegro, modern viticulture co-exists with a traditional viticulture that still maintains ancient practices and exploits local cultivars. As a result, this region provides a unique opportunity to explore processes increasing genetic diversity. To evaluate the diversity of Montenegrin grapevines and the processes involved in their diversification, we collected and analyzed 419 samples in situ across the country (cultivated plants from old orchards and vines growing in the wild), and 57 local varieties preserved in a grapevine collection. We obtained 144 different genetic profiles, more than 100 corresponding to cultivated grapevines, representing a surprising diversity for one of the smallest European countries. Part of this high diversity reflects historical records indicating multiple and intense introduction events from diverse viticultural regions at different times. Another important gene pool includes many autochthonous varieties, some on the edge of extinction, linked in a complex parentage network where two varieties (Razaklija and Kratošija) played a leading role on the generation of indigenous varieties. Finally, analyses of genetic structure unveiled several putative proto-varieties, likely representing the first steps involved in the generation of new cultivars or even secondary domestication events.
The cultivated grapevine (Vitis vinifera subsp. sativa L.) was likely first domesticated from the wild grapevine (V. vinifera subsp. sylvestris (C.C.Gmel.) Hegi) in the Transcaucasian region (modern Georgia, Armenia, and Azerbaijan) about 8,000 years ago1. Then, early cultivars spread around the Mediterranean basin following the main human migration routes during several thousand years2. During this process, certain vines were selected for their adaptation to regional conditions and their ability to overcome local biotic and abiotic stresses. These vines become stable varieties through their vegetative propagation and evolved through combining mutation and sexual reproduction events with other early-domesticated cultivars or with wild autochthonous vines2.
From ancient times to modern days, the Western Balkans have been home of important European civilizations. Illyrians, Greeks, Romans, Byzantines, Ottomans and Austro-Hungarians settled along this region, promoting the exchange of people, commodities and knowledge within close and distant regions (Near Asia, Dalmatia, Magna Graecia, etc.)3. First grapevines were introduced in the Balkan Peninsula from the East4, with the earliest-known evidences of winemaking found in Northern Greece dating back to ca. 4,300 BCE5. Ancient Greeks gradually spread grape varieties across the Western side of the Balkans and the Adriatic islands following main trading routes2,6, and Illyrians and other native populations progressively replaced the consumption of cereal-based beverages (mead, beer) with wine7. Winemaking and wine trading were two important activities for local economies at that time, as inferred from relics like those found in the necropolis of the coastal city of Budva (in modern Montenegro, dated from the fourth century BCE)8. Viticultural practices were intensified by Romans after the Illyrian Wars, as observed in Dionysian/Bacchic iconography, and in many remnants from domestic utensils used for wine transportation or consumption (pottery, amphorae, cups)7. Wine production continued during the Byzantine period, and documents like the Medieval Statute of Budva reflects the importance of grapevine cultivation in Montenegro in the Middle Ages. The Statute, dated between 1,426 and 1,442, includes the relevance of certain grapevine varieties for local wine production9,10, like the cultivar “Cratosia”, probably referring to the grape cultivar currently known as Kratošija (meaning short-neck in Montenegrin)9. Later, many vineyards were destroyed during the Ottoman period, and only some survived in remote regions in the center of the country and in several parts of the coastal regions controlled by Venice6. Winemaking practices were partially restored under the rule of Nikola I (1860–1918), which promoted wine exports to Western countries. After several millennia of genetic diversification, most of the grapevine genetic diversity generated in the Balkans was destroyed by the effect of phylloxera and mildews introduced from North America at the end of the nineteenth century2,11. As a result, many old autochthonous cultivars of the Western Balkans exist now as isolated plants or as relict populations, often represented by few specimens found in old, traditional vineyards or maintained in ex situ Vitis collections6,12, 13.
Montenegro played a major role in the long history of grape cultivation in the Western Balkans. Its highly diverse climate conditions, isolated valleys, soil types and orography create highly diverse environments that, together with its historical and geographic context, promoted the generation of a rich grapevine biodiversity14,15. Overall, grapes are grown in Montenegro over 2.800 ha, with a gross production of 22.200 t in 201716. Winemaking in Montenegro mainly relies on the production of red wine from two autochthonous grape cultivars, Vranac and Kratošija17. Kratošija was the dominant variety in the region until the phylloxera crisis, which forced the removal of many old vines and their replacement by new plant material11. In many cases, withered Kratošija vines were replaced by grafted Vranac plants, preferred by growers as it produces highly deep-colored wines17. As a result, the Kratošija cultivated area decreased, while Vranac became the most commonly grown and the most emblematic cultivar in Montenegro during the twentieth century14. Besides these two major cultivars, other less-known indigenous cultivars can be found in Montenegro and neighboring countries, including Bioka, Čubrica, Krstač and Žižak6,14,18.
Worldwide wine consumption patterns have changed radically and rapidly in recent years, influencing changes in viticulture. For a long time, grape growers selected and maintained local grape varieties to fit their viticultural practices and local climates, producing grapes that reached an appropriate concentration of sugars, acids and other compounds to make traditional wines2. On the contrary, recent market globalization has caused a dramatic erosion in the diversity of wine grapes planted across the world, moving towards the cultivation of a reduced set of “international varieties”19. Thus, the exploration and analysis of winemaking regions where old vineyards are still maintained under traditional management practices can help to understand how European viticulture developed over time before the current standardization. As exemplified in multiple regions13,18,20,21, wide surveys in traditional winemaking areas allow the identification of old genetic resources on the edge of extinction for their preservation and eventual exploitation. Grapes from unique native varieties can produce highly distinctive wines with real potential to revitalise local wine industries18,20,22, and there is a renewed global interest in local wine varieties from Old World nations to stand out in today highly standardized market. Furthermore, the analysis of these indigenous and traditional varieties has helped to successfully resolve previous doubts about cultivars parentage23,24,25, as well as to shed light on the likely origin and dissemination patterns of certain grapevine cultivars in traditional viticulture regions20,26.
The use of nuclear DNA markers, mainly microsatellites (or Simple Sequence Repeats, SSRs) and Single Nucleotide Polymorphisms (SNPs) have contributed decisively to the identification of grapevine varieties27,28. Grape varieties are maintained through vegetative propagation, and thus all the plants of any variety present the same genotype for the molecular markers used29,30, while a few molecular markers are enough to distinguish any two varieties29,31. This fact has allowed to confirm and discover many local and international synonyms (different names for one variety) and homonyms (same name for different varieties)32,33. These DNA markers have also been commonly used for determining the existence of genetic structure in sets of vines at local and wider levels34, 35. Finally, a few chloroplast DNA markers are informative enough to establish chlorotypes in Vitis vinifera, existing only four different major types36 that are maternally inherited. Chlorotypes have been useful to determine the female parent in a pedigree, and to show the multiple origins of cultivated grapevine by analyzing the distribution of chlorotypes in local sylvestris and cultivated vines37,38.
Here, we present the results of a nation-wide survey of endangered genetic resources in Montenegro, including cultivated plants (human-cared) from old orchards and vines growing in the wild. Samples were characterized by SNP and SSR markers, and unique genetic profiles were compared with international databases for proper varietal identification and for the detection of synonymies and homonymies. In addition, we analyzed Montenegrin grapevine genetic diversity and structure, and unveiled their genetic relationships. These results revealed (1) the origin of modern Montenegrin cultivars from autochthonous and/or introduced varieties, (2) the existence of anachronic proto-varieties in this region, and (3) introgression events from wild vine populations which shape the genetic structure of domesticated grapevines. This work represents a first step toward promoting wider use of indigenous and traditional grapevine varieties in the modern Montenegrin wine industry. It will contribute to the diversification and enrichment of the European wine sector.
Sampling and genetic identification of cultivated and wild grapevines in Montenegro
A broad genetic survey was performed in traditional wine regions of Montenegro. The search and sampling of cultivated plants was exclusively focused on old vines (older than 50 years, including some extremely old plants from monasteries, with written records from 1208 and 1672), located in small vineyards or orchards mostly belonging to local wine producers for self-consumption. Vines growing in the wild were mainly collected close to river or creek banks. A total of 419 vines (45 wild vines and 374 cultivated plants) were localized, georeferenced and collected throughout Montenegro (Fig. 1 and Supplementary Table S1). In addition, we included samples of 57 grapevine accessions maintained in the ex situ Vitis collection of the Biotechnical Faculty of the University of Montenegro (BTF collection), which was created from surveys performed between 1956 and 1960 to preserve local cultivated grapevine genetic resources. All the sampling sites of cultivated and wild grapevines are indicated in Fig. 1b.
Genotyping of these 476 samples at 48 SNP loci revealed 144 different genetic profiles, of which 111 corresponded to vines sampled in situ across Montenegro (either cultivated or wild). Considering these 111 genotypes, 68 corresponded to cultivated plants in ancient vineyards and 43 were sampled as wild vines (Supplementary Table S1). The comparison of the SNP genetic profiles with those stored in the Instituto de Ciencias de la Vid y del Vino-SNP database (ICVV-SNP database), and their corresponding SSR genetic profiles with international databases39,40, allowed the identification of 33 grapevine cultivars (Supplementary Table S2 and Supplementary Table S3). We detected 45 different genotypes among the 57 accessions from the BTF collection. Only 12 of these genetic profiles were also identified among the samples obtained in situ. SNP and SSR profiles allowed the identification of 25 genotypes (8 found among the in situ collected samples), which mainly corresponded to cultivars from Greece (3), Italy (3) and the former Yugoslavia (9) (Supplementary Table S1). In total, 50 genotypes could be identified and 51 could not. Considering the set of 50 identified cultivars, 29 of them corresponded to wine cultivars (58%), six to table cultivars (12%) and 15 to cultivars with a double wine/table use, according to the Vitis International Variety Catalogue40 (VIVC, www.vivc.de), (Table 1 and Supplementary Table S1). Three out of the 51 non-identified genetic profiles were found (at least) twice only in the in situ samples. Another two were found at least twice exclusively in the BTF collection, and four were found both in the in situ sampling across the country and in the ex situ sampling in the BTF collection (Table 1). All these cases suggest they have been subjected to vegetative propagation. The remaining 42 non-identified genotypes (28 from the in situ survey and 14 from the collection) were found only once in the study.
The most commonly found genotype among the 419 grapevine samples corresponded to the variety Kratošija, which was found 106 times, followed by Vranac (76), Lisica (35), Razaklija (27), Krstač (22) and Bioka (8). A high number of the 50 identified cultivars are currently considered autochthonous varieties from the Western Balkans, such as Bratkovina Bijela, Coarna Alba, Hrvatica, Kratošija, Prokupac, Vranac or Zilavka, whereas other cultivars are considered to come from Eastern countries like Greece (Heptakilo, Karystino or Muscat a Petits Grains), Turkey (Chaouch Blanc, Kadarun or Razaklija), Lebanon (Afus Ali), Armenia (Krivalja Bijela), Azerbaijan (Sysak) and Georgia (Rkatsiteli). In addition, cultivars from Western countries like France (e.g.: Bicane, Cabernet Franc and Merlot), Germany (e.g.: Mueller Thurgau), Italy (e.g.: Malvasia Bianca Lunga) and Austria (e.g.: Silvaner Gruen) were also identified, as well as bred cultivars like Angelo Pirovano and Perlona, and Vitis spp. interspecific hybrid direct-producers like Isabella and Varousset.
All the wild plants hold unique genotypes, but for two genotypes (named Wild MNE 273 and Wild MNE 359) that were found twice in the wild (Supplementary Table S1). Wild MNE 359 samples were found extremely close in the municipality of Gornji Ulići, so it is probable that both samples were inadvertently taken from different branches of the same individual. Wild MNE 273 samples were found at the two sides of a road in Crmnica, suggesting the occurrence of natural vegetative multiplication41.
Lastly, chlorotype analyses revealed a majority of cultivars bearing chlorotypes C (43 genotypes, 42.6%) and D (33, 32.7%), followed by chlorotypes A (13, 12.9%) and B (3, 3.0%). On the contrary, most plants sampled in the wild bear chlorotype A (30 genotypes, 69.8%), followed by chlorotypes D (7, 16.7%) and C (6, 13.9%) (Supplementary Table S1).
Population structure analysis of cultivated and wild grapevines in Montenegro
To understand the population structure of Montenegrin grapevines, 144 non-redundant genotypes were profiled with 192 additional SNP loci. It yielded satisfactory results for 131 genotypes, which were used for further population structure, genetic diversity and pedigree analyses (Supplementary Table S2). The UwNJ distance tree generated two main clusters (Fig. 2a), which mostly reflected the sampling origin of the genotypes included in the analysis. Thus, one of the clusters included most of the genotypes collected as wild vines, whilst the other included most of the genotypes collected as cultivated plants. Interestingly, two genotypes sampled as cultivated plants (named Kratošija Put Ka Zagarcu and Nepoznata Crmnica) located in the cluster of wild vines, whereas two genotypes collected in the wild (Wild MNE 362 and Wild MNE 475) located in the cluster of cultivated vines. PCoA results supported this major genetic differentiation, with PCoA1 clearly separating the genotypes collected in the wild from those collected as cultivated vines (Fig. 2b). Clustering results for Kratošija Put Ka Zagarcu, Nepoznata Crmnica, Wild MNE 362 and Wild MNE 475 were also supported by the PCoA.
On the other hand, STRUCTURE analysis and the ΔK criterion suggested K = 2 as the optimal uppermost hierarchical level of structure for the set of 131 non-redundant grapevine genetic profiles (Supplementary Figure S1a). At this level of structure, a clear separation between the genotypes sampled as cultivated or wild was also obtained. Considering a membership coefficient (q-value) threshold of 0.5 for group assignment, one genetic group (Str1.1; n = 87) is mainly formed by samples collected as cultivated plants (81 genotypes, 93.1%). The other genetic group (Str1.2, n = 44) is mainly formed by genotypes sampled as wild vines (37 genotypes, 84.1%) (Fig. 2c and Supplementary Table S2). As previously noted, Kratošija Put Ka Zagarcu and Nepoznata Crmnica were found in the genetic group Str1.2 with ancestry q values over 0.95, whereas Wild MNE 362 and Wild MNE 475 were found in Str1.1 with ancestry q values over 0.98. Interestingly, two grapevine cultivars reported as progenitors of many other relevant varieties (Cabernet Franc and Savagnin), clustered in Str1.2 with most of the wild vines included in this work, with membership coefficients of 0.530 and 0.505, respectively (Supplementary Table S2).
Genetic diversity parameters were individually calculated for Str1.1 and Str1.2, excluding clearly admixed genotypes by using a more stringent threshold for group assignment (q values > 0.6). Significant differences (p ≤ 0.05) between the non-redundant genotypes assigned to Str1.1 and Str1.2 were found for all the parameters (Table 2). The group of cultivated plants (Str1.1, n = 82) showed significantly higher values of expected (UHe) and observed (Ho) heterozygosity than the group of wild vines (Str1.2, n = 38), while the fixation coefficient (FIS) was close to zero for both genetic groups. The FST value between Str1.1 and Str1.2 populations was 0.131.
Additional levels of genetic stratification in the set of cultivated vines were tested through a second STRUCTURE analysis, considering the 87 non-redundant genetic profiles assigned to Str1.1 (q value > 0.5) and the four genotypes sampled as cultivated but assigned to Str1.2 with q value < 0.6 (Savagnin, Loznica-2, Cabernet Franc and Bijela Nepoznata Jelenak, Supplementary Table S2). The ΔK criterion reported similar values at K = 2 and K = 3 (Supplementary Figure S1b). At K = 2, considering a critical q value of 0.70 for group assignment, the two genetic groups included 54 genotypes (Fig. 3a and Supplementary Table S2). Str2A.1 contained 31 cultivars of diverse origin, but most of them ancient and progenitors of many other relevant grapevine varieties (e.g.: Cabernet Franc, Heptakilo, Muscat a Petits Grains, Savagnin). Str2A.2 (n = 23) mostly included cultivars considered autochthonous from the Western Balkans (e.g.: Coarna Alba, Čubrica, Kratošija, Raćeška, Vranac). This level of structuring was supported by the UwNJ and PCoA analyses (Fig. 3b,c), with genotypes assigned to Str2A.1 or Str2A.2 separated by PCoA1 and PCoA2 (Fig. 3b), and clustering in opposite branches of the UwNJ distance tree (Fig. 3c). At K = 3, three genetic groups clustering 30 genotypes were obtained (q value > 0.70 for group assignment) (Supplementary Figure S2a and Supplementary Table S2). Str2B.1 contained 7 genotypes (“RAZ-group”), related to the ancient Turkish cultivar Razaklija (prime name in VIVC: Parmak Cerven40). Str2B.2 included 6 genotypes native to the Western Balkans (“KRA-group”), such as Kratošija and Vranac. Interestingly, these 6 genotypes were assigned to Str2A.2 when considering K = 2. Str2B.3 is formed by 17 genotypes, including internationally renowned cultivars like Cabernet Franc, Merlot, Mueller Thurgau, Savagnin or Silvaner Gruen (“INT-group”), and all were assigned to Str2A.1 when considering K = 2. PCoA results supported the genetic differentiation indicated by STRUCTURE at K = 3, with genotypes assigned to RAZ-group, KRA-group and INT-group clustering in different regions of the plot (Supplementary Figure S2b). Nevertheless, we did not obtain a clear separation of these 30 genotypes in the UwNJ distance tree, as genotypes assigned to the RAZ-group were scattered in the tree (Supplementary Figure S2c).
Parentage analysis of cultivated and wild grapevines in Montenegro
The 131 non-redundant grapevine genetic profiles were merged with those stored in the ICVV-SNP database for a wide search of possible first-order kinship relationships using 240 SNP-profiles. All reliable trios (mother + father + offspring) and duos (parent-offspring) involving (at least) one of the genotypes found in this work can be found in Supplementary Tables S4A and S4B, together with their LOD values and the number of mismatching loci. The most relevant relationships resulting from our analysis are shown in Fig. 4. Global results showed 25 compatible trios, with high LOD values (> 62.50) and a maximum of 2 mismatching loci. Our results confirm the pedigrees previously suggested for Clairette Mazel42, Plavina Crna42 and Župljanka43. Results indicate a leading role of Razaklija and Kratošija in the generation of Montenegrin grapevine diversity, being involved as progenitors in 14 and 12 pedigrees, respectively. Together they have six offspring, including two genotypes sampled as wild vines (Wild MNE 362 and Wild MNE 475, with ancestry q-values over 0.98 in Str1.1). In addition, we detected six compatible parent–offspring relationships for Kratošija, and five for Razaklija, including other two genotypes sampled as wild vines (Wild MNE 282 and Wild MNE 285, with ancestry q values ca. 0.50 in Str1.1). Pedigree analysis also allowed discovering the genetic origin of Vranac, currently the most emblematic variety in Montenegro. Our results confidently support that Vranac derived from a hybridization event between cultivars Duljenga and Kratošija (LOD = 85.10), with no mismatching loci in the proposed trio. This pedigree was also supported by an additional set of 20 SSR markers (Supplementary Table S5), and chlorotype analyses identified Duljenga as the female progenitor of Vranac (Supplementary Table S4A). In addition, we identified one cultivated genotype (Čubrica-2) as the compatible result of a self-cross of cultivar Čubrica (Fig. 3). No full trios were found among the wild vines, but two reliable duos were detected in two different populations: in Lesendro Fortress (involving Wild MNE 275 and Wild MNE 276) and in Orasi (involving Wild MNE 363 and Wild MNE 364) (Supplementary Table S4B).
Modern Montenegrin viticulture and winemaking industries co-exist with a significant number of old and small vineyards managed through traditional techniques14,15. Such viticultural tradition was shaped by a unique geographic position, terrain fragmentation and history. This traditional activity provides a fascinating window into this relict viticulture, and represents a unique opportunity to explore how viticulture evolved before wine market standardization. To this aim, we have studied the diversity of the traditional Montenegrin grapevine genetic pool through the most extensive search and sampling of cultivated and wild grapevines ever done in the country so far.
We found an unexpectedly high genetic variability in the cultivated set, detecting 101 genotypes. Fifty genetic profiles were identified as well-known wine, table or multiple-use grape cultivars, most of which were Western Balkan autochthonous varieties. Kratošija and Vranac, the most important cultivars for current Montenegrin viticulture, were also the two most commonly found genotypes across old Montenegrin vineyards, reflecting their relevance in the past century10,17. In addition, we identified a significant number of foreign cultivars, supporting historical reports that indicate multiple introduction events in the country from diverse regions at different times and with different aims6. Cultivars from Eastern regions are possibly a consequence of viticulture dissemination from middle-East regions to Western European countries2, whilst cultivars from Western regions mostly derive from the intense exchange of plant material happened during the second half of the nineteenth century to fight grape phylloxera, mildews, and other pests2.
Furthermore, we found 51 unidentified genotypes, cultivated in many cases as homonyms, under wrong cultivar names. Nine of them were found in at least two different sampling points (including the BTF collection), indicating their vegetative propagation and, therefore, that they are true cultivars, on the brink of extinction. Interestingly, some of them were found to be offspring of other well-known local cultivars (e.g.: Belka, Crni Krstač), indicating a Montenegrin origin. The remaining 42 non-identified genotypes only appeared once in the sampling of cultivated plants. Some of them may represent true varieties too, as supported by the genetical identification of descendants for some of these genotypes (e.g.: Crna Nepoznata Malisne for Volovina Iz Opeke). As they were sampled only once, these 42 genotypes are examples of critically endangered Montenegrin grapevines. Besides, a remarkable particularity of Montenegro viticulture is the current presence of an important number of putative ‘proto-varieties’. This term is used here to designate plants that have been only cultivated by local grape growers, i.e. plants that directly grow from seeds, or have been multiplied through cuttings just once, from the place where the seed germinated to the orchard. Eventually, they could be multiplied and distributed, becoming varieties. This is how most of the varieties originated in the past, but this process is not active anymore in Western European regions.
Strikingly, an important number (33) of the genotypes conserved in the BTF collection (from a sampling of the 1960’s) was not found among the in situ samples studied in this work, indicating fast disappearance of ancient varieties, and underscoring the relevance of these surveys to preserve local genetic resources from extinction19.
Sexual reproduction is known to be the prime process in the generation of new grapevine genotypes2, and autochthonous varieties are the main source for shaping local grapevine diversity. Previous works have identified intricate parentage networks linking multiple grapevine cultivars1, 42, and major founders have been detected in many traditional winemaking regions, such as Pinot and Gouais Blanc (synonym Heunisch Weiss) in Burgundy and in Northeastern France44, Cabernet Franc in the Aquitania region of Bordeaux45, Hebén in the Iberian Peninsula46,47, and Sangiovese and Garganega in the Italic Peninsula48. Here, we found that Razaklija and Kratošija played a key role shaping the grapevine genetic pool of Montenegro, participating in a high number of the discovered trios and duos. Razaklija is a Turkish table grape cultivar existing in many grapevine collections under multiple synonyms40, and is a well-known genitor of several Albanian cultivars12. We found it compatible as the female parent in 14 full pedigrees (as expected, given its functional female flowers40). Interestingly, several grapevine varieties with female flowers have been previously reported to have an important contribution in the establishment of local genetic networks, like Hebén and Marufo in the Iberian Peninsula38,47. Unlike hermaphrodite plants, females need to cross-pollinate to produce descendants, process that increases genetic diversity and increases hybrid plant vigor38, which could have favored their selection as seed donors by early farmers to ensure grape production.
Kratošija (also known as Zinfandel in California, Primitivo in Italy and Tribidrag, Pribidrag or Crljenak Kastelanski in Croatia32) is a cultivar grown in the Western Balkans for centuries. Nevertheless, its place of origin is controversial, and it has changed as new evidence was added: first from USA to Italy49, and then to Croatia, where old references and a reduced number of plants with a matching genotype were found under the name Crljenak Kastelanski50. More recently, the first molecular analysis of Kratošija proved that it has the same genotype32. In our study we identified 106 plants of Kratošija in old Montenegrin vineyards, and we found it to be the genitor (trios and duos) of almost 20 grapevine genotypes cultivated in Montenegro. In accordance with its ancient cultivation in this region, multiple Kratošija biotypes differing in traits like grape yield, cluster compactness or cluster size have been found in Montenegro14. In addition, early historical references already indicate the widespread use of Kratošija grapes for traditional winemaking in Montenegro, as reported in the Medieval Statute of Budva9, and reflected in folklore traced back to the eighteenth century10. Although definitive proof to establish the birthplace of an ancient variety cannot be provided, our results and the mentioned evidence support that Kratošija might have originated somewhere within Montenegro, agreeing with previous findings51.
Vranac is considered an old autochthonous Montenegrin grape variety used for local red wine production for centuries10, and it was already described as a black-berried Montenegrin cultivar in the Traité général de viticulture of Viala and Vermorel (1909) under the name “Vranatz-Krstatch”52. Previous reports indicate the existence of a first-degree relationship between Kratošija and Vranac32. Here, we found the full pedigree for Vranac, being the result of a compatible offspring between Duljenga and Kratošija. Duljenga was exclusively found in the BTF collection, and there are no reliable documents indicating the origin of this cultivar. Its presence in the BTF collection and its role as Vranac progenitor indicates that it was cultivated at some time in Montenegro.
As nicely summarized by Miller and Gross, the domestication process in perennial fruit crops produces a continuum of plant populations, which range from exploited wild individuals to initial domesticates to cultivated populations53. In agreement with surveys done in wider frameworks20,34,54, the clear division between Str1.1 and Str1.2 seems to correspond to the two V. vinifera L. subspecies: sativa and sylvestris. However, it does not completely correspond with cultivated and wild conditions, as some intermediate genotypes generating a continuum were found. Population analyses allowed us to identify two exploited sylvestris plants (Kratošija Put Ka Zagarcu and Nepoznata Crmnica) collected from vineyards and yet genetically of the subsp. sylvestris. These plants bear chlorotype A and unique genotypes with no matches in the consulted databases, indicating they arise from sylvestris vines that were vegetatively propagated. These exploited sylvestris plants could be also considered as proto-varieties and, unlike the cases mentioned above, may represent novel active domestication events. Previous studies also report the in situ domestication of sylvestris autochthonous vines for grape production38,55, suggesting the existence of secondary domestication events in areas of grapevine cultivation at different times. The existence of these events is an open issue in grapevine research56, being difficult to ascertain if current cultivated grapevines are the result of multiple domestication events37,54 or a combination of a primary domestication event and multiple local introgression events during the geographic expansion of the crop to new locations.
We found a set of cultivated plants with similar percentages of ancestry to the sativa and sylvestris genetic subgroups (like Bijela Nepoznata Jelenak and Loznica-2). As stated by Stebbins in 1950, any single valuable hybrid individual, once obtained, can immediately become the progenitor of a new variety and can be perpetuated indefinitely53. In addition to Bijela Nepoznata Jelenak and Loznica-2, this fact was also observed with two widespread cultivars, Cabernet Franc and Savagnin (synonym Traminer). These two cultivars are genitors of a high number of cultivars (20 for Cabernet Franc and 70 for Savagnin40), and recent paleogenomic analyses indicate that Savagnin could have been uninterruptedly cultivated in Europe for (at least) 900 years4. The number of sexual cycles separating domesticated individuals from their wild ancestors is suggested to be low in perennial plants with clonally-propagated varieties53, which might explain the high level of wild ancestry detected in these two ancient varieties. In that sense, Bijela Nepoznata Jelenak and Loznica-2 are examples of active pathways to introgress genomic regions of subsp. sylvestris into new varieties.
Finally, we detected six individuals sampled as wild vines with higher sativa than sylvestris genetic composition (four of them bearing chlorotype C). Two of them (Wild MNE 362 and Wild MNE 475) show extremely high coefficients of ancestry in the genetic subgroup of cultivated varieties and are compatible offspring of Kratošija and Razaklija, which classifies them in the sativa subspecies. Possibly, they have derived from seeds produced in vineyards and dispersed into neighboring natural environments by humans or animals. The presence of naturalized vines from grapevine cultivars, rootstocks and/or direct-producer hybrids is well documented in alluvial European forests57,58, and they are currently considered as invasive plants capable of contaminating natural wild grapevine populations. Thus, the cultivated vines (human-cared vines) do not always belong to the sativa subspecies, just as vines growing wild without cares do not always belong to the sylvestris subspecies.
Our in-depth study of V. vinifera L. diversity in traditional viticultural regions across Montenegro provided insights not only about the existing genetic diversity, but also on how it was generated. The exploration of these unique regions, shaped by specific geographic and historical conditions, made it possible to detect different stages in the domestication process of this woody perennial, providing useful information on the processes of evolution underlying the generation of the current cultivated varieties. The study allowed us to conclude that the high diversity observed for Montenegrin varieties arose from ancient pathways to generate genetic diversity that are still active. These pathways involved the cultivation of new grape plants from seeds which increased genetic variation by: (1) the spontaneous sexual hybridization among cultivars introduced from different regions, (2) the spontaneous sexual hybridization between sylvestris and sativa grapevines, and (3) the vegetative reproduction of local sylvestris plants for grape production.
Materials and methods
Collection of plant material
A total of 419 cultivated (374) and wild (45) grapevines were sampled throughout Montenegro, focusing in old, traditional vineyards dispersed in the two major viticulture regions of the country (the Lake Skadar basin and the coastal region). Samples were collected in several exploration trips in 2013, 2014, 2016 and 2017, and GPS coordinates were recorded for each sampling site (Fig. 1 and Supplementary Table S1). Young leaves were collected on site for each sample, and kept in ice until storage at − 80 °C for DNA extraction and genotyping. In addition, a set of 57 samples from the ex situ Vitis collection of the Biotechnical Faculty of the University of Montenegro (BTF collection) was included as a reference to aid in the identification of autochthonous cultivars. Cuttings from cultivated samples were taken in winter, grafted and planted in a new repository created by 13 Jul Plantaže to preserve this material. Cuttings from wild samples will be collected in upcoming seasons, and treated as described to be part of this repository.
DNA isolation, genotyping and varietal identification
DNA was isolated from frozen leaves as previously detailed59. All samples were initially profiled for a core set of 48 nuclear SNP markers29, using the SNP-genotyping services provided by the Spanish Centro Nacional de Genotipado (CEGEN), as recently described38 or the Sequencing and Genotyping Unit of the University of the Basque Country, using Fluidigm technology. Three chloroplast SNPs that allow to distinguish the main grapevine chloroplast haplotypes (A, B, C and D)36 were used to determine sample chlorotype. Non-redundant genetic profiles (144) for the 48 SNPs were pair-wise compared with the ICVV-SNP database for cultivar identification, which includes the genetic profiles of more than 2,800 non-redundant genotypes for 48 SNPs. In those cases in which the SNP profile did not match with an identified variety in the ICVV-SNP database, samples were additionally characterized with 9 SSR markers (VVS2, VVMD5, VVMD7, VVMD25, VVMD27, VVMD28, VVMD32, VrZAG62 and VrZAG79) in the genotyping platform of the Centro de Investigación Biomédica de La Rioja (CIBIR). Profiles of SSRs were compared with those stored in the VIVC40 and the European Vitis Database39. All 144 non-redundant genotypes were profiled for an additional set of 192 SNP markers60 using the genotyping platforms stated before. Unfortunately, no satisfactory results were obtained for 13 genotypes, so 131 non-redundant grapevine genotypes with genetic profiles for 240 SNP loci were used for population structure and genetic diversity analyses. SNP and SSR analyses were conducted as previously described38, 61,62.
Analysis of population structure and genetic diversity parameters
An Unweighted Neighbour-Joining (UwNJ) distance tree and a Principal Coordinate Analysis (PCoA) were calculated to explore the relationship between the cultivated and wild grapevine genotypes collected across Montenegro. To this aim, a dissimilarity matrix with 10.000 bootstrap steps was calculated using the DARwin software package v. 6.0.2163 for 131 non-redundant genotypes considering 194 SNPs (SNPs with missing data in 10 or more grapevine genotypes were discarded). This dissimilarity matrix was used for PCoA and UwNJ analyses; the UwNJ tree was constructed on the basis of 1.000 bootstrap replicates.
Then, the Bayesian clustering method implemented in the STRUCTURE v.2.3.4 software64 was used to infer the number of genetic groups present in the set of 131 non-redundant grapevine genotypes. Here, we tested the existence of genetic structure considering a number of hypothetical genetic groups (K) ranging from 1 to 10, using a cycle of 100.000 burn-in steps followed by 150.000 Markov Chain Monte Carlo repetitions. To assess the consistency of the results, 10 runs per K value were performed, each one considering an admixture model with correlated allele frequencies among populations. The most likely number of genetic groups was set using the ΔK criteria65 implemented in STRUCTURE HARVESTER v. 0.6.9466, and CLUMPP v.1.1.267 was used to align the 10 different runs. Genotypes were assigned to a genetic group considering a threshold of q ≥ 0.50. Results were graphically represented by means of the web-based software STRUCTURE PLOT v.2.068.
Genetic diversity parameters (number of effective alleles (Ne), information index (I), observed heterozygosity (Ho), unbiased expected heterozygosity (UHe) and fixation coefficient (F)) were calculated for the genotypes assigned to the two genetic groups identified by STRUCTURE, discarding those identified as Vitis spp. interspecific hybrids for their non-vinifera genetic background. To avoid the effect of individuals with a similar q ancestry value in both genetic groups, we set a q value threshold of 0.60 for group assignation. Thus, cultivated and wild genetic groups included 82 and 38 non-redundant genotypes, respectively. The FST statistic was used to analyze the genetic distance between cultivated and wild genotypes. Non-polymorphic markers (5 and 26 for the cultivated and wild subsets, respectively) were excluded for diversity parameters estimation. Calculations were performed using GenAlEx v. 6.569. As previously indicated61, mean values of these metrics were subjected to a t test analysis to detect significant differences between both groups, considered significant at p < 0.05. This analysis was performed using IBM SPSS Statistics v.25.0 (Chicago, IL, USA).
Lastly, we performed a second round of hierarchical STRUCTURE analysis to evaluate additional levels of genetic stratification70, focusing on the inferred genetic group of cultivated grapevines (91 non-redundant genotypes). The same procedure described above was used, but we selected a more stringent q value (0.70) as group assignation threshold. The consistency of the genetic structure results obtained in this second round was evaluated through a PCoA and an UwNJ analysis, performed as detailed before.
Non-redundant grapevine genotypes were merged with those of the ICVV-SNP database to complete 1921 genotypes with data for 240 SNPs and analyzed to detect possible first-order kinship relationships (trios and parent–offspring pairs), using the likelihood-based method implemented in Cervus v. 3.071 as previously detailed38,61. The likelihood of each detected trio and parent–offspring pair (duo) was evaluated considering the natural logarithm of the overall likelihood ratio (LOD) score, and a maximum number of mismatching loci of 1 or 2 SNPs for duos and trios, respectively. For each trio, chlorotypes were used to determine which of the putative parents acted as mother, according to the maternal transmission of chloroplasts in grapevine36.
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We thank Miguel Angulo, Silvia Hernáiz, Beatriz Larreina and Nachi Montemayor (ICVV) for their technical assistance. We thank Elena Domínguez-Garrido and Jana Aguirre from the Centro de Investigación Biomédica de La Rioja (CIBIR) for their technical assistance in SSR genotyping.
The authors declare no competing interests.
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Maraš, V., Tello, J., Gazivoda, A. et al. Population genetic analysis in old Montenegrin vineyards reveals ancient ways currently active to generate diversity in Vitis vinifera. Sci Rep 10, 15000 (2020). https://doi.org/10.1038/s41598-020-71918-7
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