Climate change is expected to accelerate the microbial degradation of the many extraordinary well-preserved organic archaeological deposits found in the Arctic. This could potentially lead to a major loss of wooden artefacts that are still buried within the region. Here, we carry out the first large-scale investigation of wood degradation within archaeological deposits in the Arctic. This is done based on wooden samples from 11 archaeological sites that are located along a climatic gradient in Western Greenland. Our results show that Ascomycota fungi are causing extensive soft rot decay at all sites regardless of climate and local environment, but the group is diverse and many of the species were only found once. Cadophora species known to cause soft rot in polar environments were the most abundant Ascomycota found and their occurrence in native wood samples underlines that they are present locally. Basidiomycota fungi were also present at all sites. In the majority of samples, however, these aggressive and potentially very damaging wood degraders have caused limited decay so far, probably due to unfavorable growth conditions. The presence of these wood degrading fungi suggests that archaeological wooden artefacts may become further endangered if climate change leads to more favorable growth conditions.
Archaeological deposits in Greenland often have a high abundance of wooden artefacts that primarily derive from Canadian or Siberian driftwood1. Even though only a fraction of the country’s archaeological deposits have been excavated, numerous spectacular finds have been made and the potential for further discoveries is anticipated to be high2,3. The buried wooden artefacts may, however, be under serious threat from climate change. Rising air temperatures cause longer periods of ground thawing and altered precipitation patterns4,5, and this may result in drying of near-surface layers and thereby increasing oxygen availability. Both factors are expected to accelerate the degradation of buried organic materials6,7 and lead to a significant reduction in the number of well-preserved archaeological deposits within the next century7,8,9.
Currently very little is known about the microbial and fungal communities in Arctic archaeological deposits and consequently it is difficult to foresee where and when archaeological wooden artefacts will be increasingly degraded following climatic changes. Studies of non-archaeological wood show that slow growing Ascomycota causing soft rot are the most common wood degraders in Polar Regions10,11,12,13,14,15. Soft rot fungi degrade the secondary cell wall but leave behind the compound middle lamella (CML)16,17. This results in preservation of a skeleton of the wood structure but with significant loss of strength. However, as long as the wood is kept waterlogged or frozen, the 3D structure and surface details of the wood may be well preserved18. Wood-inhabiting Basidiomycota fungi are also known to be present in the Polar Regions19,20,21,22. Basidiomycetes cause white and brown rot, which result in extensive damage after a relatively short time when the environmental conditions are suitable. This more aggressive degradation is fatal for archaeological wooden artefacts since all cell wall layers in the wood structure are degraded and consequently artefacts can be totally disintegrated12,23,24,25.
To date, the degradation of buried archaeological wooden artefacts has only been studied at one site in the Arctic6. Here optical microscopic analyses showed that archaeological wood samples taken from the permafrost were almost perfectly preserved, whereas clear signs of degradation by soft rot and a significant loss of mass were observed in samples taken from the active layer, which is the upper part of the soil that thaws every summer6.
Here we perform the first large-scale assessment of the distribution of wood degrading microbial communities associated with archaeological wooden artefacts in the Arctic. This is done based on samples of wooden artefacts collected from 11 contrasting archaeological deposits located along a north/south climatic gradient in Western Greenland. The aim is to determine the main types of wood degraders in the active layer and to identify potential connections between climatic conditions and distribution of the various wood decay fungi.
Study sites and samples
Our wooden samples from the 11 different archaeological sites (Fig. 1, Table 1) span over the cultures of Saqqaq (2,500–800 BC), Dorset (300BC–600AD), Thule (1,300AD–present), and the Norse settlers (985–1,450 AD). The sites were chosen in order to include samples from different cultures and time periods. The study sites are located along a north/south gradient with respect to air temperatures and soil temperatures (Fig. 1a) and are furthermore exposed to very different precipitation regimes (Fig. 1b). The variation in the mean annual thawing degree days (TDD) and the duration of the thaw period also varies greatly between the sites (Table 1).
Excavations and test trenching were carried out at nine study sites in the period 2009–2016. In total 45 archaeological wooden samples were collected from the active layers at these sites (referred to as buried archaeological samples) (Supplementary Table S1 online). When a large number of samples were available at a site, the most degraded wooden artefacts were sampled in order to study the worst case state of preservation. In addition, 21 archaeological wooden samples that were partly exposed to ambient air due to either erosion or prior archaeological excavations (in old profiles) were sampled at three sites (referred to as exposed archaeological samples) (Supplementary Table S1 online). Exposure to ambient air is expected to accelerate the microbial decay and possible favour wood-inhabiting Basidiomycota fungi. The samples therefore served as worst-case scenario for wood preservation associated with the active layer at the sites. Six samples of exposed historical wood were collected directly on the soil surface at two sites (referred to as exposed historical samples). The historical samples originate from imported conifer species and were left at the two sites approximately in 1970 and 1950. These samples served as measure/indicator of fungi associated with exposed non-native and non-driftwood conifer. Furthermore, 12 samples of native dead Salix glauca L. were collected directly on the soil surface at four sites (referred to as native dead samples). These samples served as an indicator for local fungi associated with dead native grown hardwood shrubs.
We determined wood genera/species for each wood sample by examining the anatomical characteristics with transmission light microscope. Results show that 87% of the 45 buried archaeological wood samples were conifers of the genera Picea (51%), Larix (18%) and Pinus (4%) (Supplementary Table S1 online). Some of the samples (13%) were heavily decayed and could only be identified as conifer wood. Furthermore, the distinction between Picea and Larix was somewhat uncertain since these two wood genera have great anatomical resemblance and can only be distinguished with certainty by differences in bordered pitting in ray tracheids26. However, the often advanced stages of microbial decay made pit anatomy uncertain. The remaining 11% of the buried archaeological samples were Salix. The 21 exposed archaeological wood samples were all conifer of the genera Picea (38%), Larix (43%) and Pinus (19%), and the exposed historical wood samples were of the genera Picea (33%) and Pinus (67%) (Supplementary Table S1 online).
Visual decay analysis
The state of preservation of the buried archaeological wood samples was first evaluated by macroscopic examination of surface decay (Fig. 2). This revealed that 91% of the samples had some distortion of the surface which in itself entails lower scientific value as tool marks and surface decoration may be affected. Subsequently, the buried wood samples were evaluated by microscopic examination of cross sections cut from the surface towards the inner part of the samples to determine the types of decay present, the dominant decay type present, and the degree of degradation. All but four samples were decayed by soft rot which was the dominant decay type in 84% of the samples (Figs. 2, 3a,b). Brown rot was found in 42% of the buried archaeological samples, but was only the dominant decay type in 13% of the samples (Figs. 2, 3c). Only one of the buried archaeological samples was attacked by selective white rot. However, all but two samples had areas within the wood structure where all cell wall material, including the compound middle lamella, was missing leaving small holes in the material along with the otherwise dominating soft rot decay (Fig. 3d). This decay pattern could result from pockets of simultaneous white rot but the decay of the CML could also result from mechanical damage due to thaw/freeze cycles of the fragile material heavily decayed by soft rot. The degree of degradation was generally high with 62% of the samples in an advanced state of decay. Visual decay analysis of the 21 exposed archaeological wood samples and the six exposed historical wood samples showed decay patterns that were very similar to the ones found in the buried archaeological samples (Supplementary Fig. S2 and S3 online).
DNA identification of fungi isolated from sampled wood material
Incubation of wood samples on culture media followed by isolation of fungal mycelia and DNA sequencing for identification revealed a diverse group of Ascomycota as the dominant fungal type, with many of the species found only once (Supplementary Table S4 online). Among the Ascomycota found, the wood degrading soft rot fungus Cadophora sp. were more frequently isolated than other genera. Cadophora sp. were found at eight different sites, in 15 different wood samples, and in all four types of sample material (buried and exposed archaeological wood, exposed historical wood, and native dead wood). Other Ascomycota fungi known to cause soft rot in wood were found at seven different sites in a smaller number of wood samples (all sample types) including Chaetomium, Cladosporium, Lecythophora, Leptodontidium, and Phialophora. Strains known to cause soft rot in wood were not found at Nuulliit, Kilaarsarfik, and Kangerdluarssugssuaic, even though soft rot was observed in the wood samples. Ascomycota fungi found in a high number of samples (in eight to 11 wood samples at three to six different sites) includes Penicillium, Patinella, Xenopolyscytalum, Pseudeurotium, and Phialemonium. However, none of these five Ascomycota are known to cause soft rot in wood. Xenopolyscytalum, Patinella, and Phialemonium were only found in archaeological wood samples, and Pseudeurotium was found in the archaeological wood samples except for one isolated from a wood sample of exposed historical wood (Fig. 4 and Supplementary Fig. S5–S7 online).
Only a very limited number of filamentous Basidiomycota were isolated with most of the species found only once (Fig. 4, Supplementary Table S4 and Fig. S5–S7 online). One buried archaeological wood sample contained Tremellales, two exposed archaeological wood samples from two different sites contained Hohenbuehelia and Lentinellus, two exposed historical samples from two different sites contained Dacrymyces and an unknown Basidiomycota species, and four native dead Salix samples contained Fomes, Lentinellus, and Mycena.
These findings only represent cultures that are alive or able to germinate under the given growth conditions. We therefore also performed extractions of gDNA followed by real-time PCR DNA analysis in order to obtain quantitative determination of Basidiomycota DNA in the sample material. gDNA extraction was performed directly from 29 of the buried archaeological wood samples, 15 of the exposed archaeological wood samples, all six exposed historical wood samples, and all 12 native dead Salix samples. The results show that all wood sample categories and wood from all sample sites contained Basidiomycota DNA but with a very variable concentration (Fig. 5). Only very few sub samples did not contain any Basidiomycota DNA (11 of the buried archaeological samples and one native dead Salix sample). The samples of native dead Salix and exposed historical wood contained notably more Basidiomycota DNA than both types of archaeological wood samples (exposed and buried) (Fig. 5). The quantification method is not selective towards wood decaying Basidiomycota and will therefore include quantification of Basidiomycota yeast and mycorrhiza that do not degrade wood, if present. Therefore, gDNA extracts with a high-quality melting curve were used for sequencing for fungal species identification. The 39 analysed subsamples (21 different wood samples from seven sites including all sample types) revealed that all the identified Basidiomycota fungal genera are from genera that are known to cause wood decay (Supplementary Table S8 and S9 online).
We investigated 66 archaeological samples of wood (buried or exposed) from 11 different archaeological sites in Western Greenland. The majority of samples (93%) were species of conifer that have not grown naturally in Greenland during at least the Holocene27. Not surprisingly, our results thereby underline the importance of driftwood as a source of raw material.
Ascomycota was the dominant decay type in more than 80% of the samples, not only in the buried archaeological wood, but also in the exposed archaeological samples and in the historical wood samples exposed directly on the ground for approximately 50 or 90 years. In most cases, the observed soft rot decay was very extensive. Two thirds of both the buried and the exposed samples were decayed to an advanced stage where the entire secondary cell wall was decayed. We specifically targeted the most degraded wooden artefacts at each site and thus our results do not reflect the average state of preservation but rather a worst-case scenario. Nevertheless, they clearly confirm that Ascomycota are readily abundant in Greenland’s archaeological deposits and that soft rot may cause significant damage to wooden artefacts in all parts of the country and across a substantial climatic gradient. Cadophora species were the most abundant of all the Ascomycota. It was found at eight of the 12 locations sampled and species of Cadophora are known to cause soft rot in many different polar environments12,14,21,28,29. Their high frequency of isolation in our study suggests that they are common and likely represent an important group of indigenous wood decay fungi well adapted to the extreme Arctic environment that attack archaeological and historic woods in Greenland. We also found a high frequency of Patinella (five sites), Xenopolyscytalum (four sites), Pseudeurotium (four sites), and Phialemonium (three sites) almost exclusively associated with the archaeological sample material buried or partly buried in the soil. These genera are rarely encountered30,31 but due to the high frequency, the close association to the buried material, and the advanced soft rot decay of the sample material from all sites, it is speculated that these species could also be causing soft rot decay in the archaeological wood material. Additional research is needed to determine their decay potential.
Driftwood is decayed by a rich variety of soft rot fungi14 and may already be colonized during waterborne travel or when lying at the coastline. This means that the wooden samples could have been colonized with soft rot when first picked up by humans centuries or even millennia ago. However, five of the six exposed historical (non-driftwood) samples and the exposed native dead Salix samples were also dominated by soft rot. The occurrence of Cadophora in native wood samples in this study underlines that they are present locally and do not need to be imported with the wood. Thereby our results indicate that soft rot fungi are present in surface layers of Arctic soils and readily colonize any wood whether it is historic or modern from native and non-native trees and shrubs. This is supported by studies by Blanchette et al.14 and Jurgens et al.29. Blanchette et al.14 show that the majority of fungi found in driftwood at sites in Greenland, Iceland, and the Siberian Lena Delta are similar to the native population. Jurgens et al.29 show that even non-mineralized fossil wood found in proglacial soil at Ellesmere Island, Canada is decayed by local soft rot fungi when in direct soil contact. We therefore conclude that the major source of these fungi are from indigenous populations, and only few fungi might have travelled large distances with the driftwood or were spread over large distances by humans or animals. The studied wood decaying fungi are part of the natural degradation process that has evolved in the Arctic over time. The fungi utilize carbon sources in the environment weather it is woody plants like Arctic willow or driftwood that arrives from great distances away. The wood is colonized from airborne spores or from direct contact with fungal mycelium in soils.
Our quantitative DNA analysis showed that Basidiomycota DNA was present in nearly all wood samples but in very variable amounts. Presence of wood decaying Basidiomycota was confirmed in our visual decay analysis. In total 42% of the buried archaeological samples showed signs of brown rot decay and 40% of the samples had some or advanced CML decay, which could result from simultaneous white rot decay. In addition, Basidiomycota associated with wood decay were found in a smaller portion of the samples selected for fungal species identification. This confirms that wood decaying Basidiomycota are abundant in Greenland’s archaeological deposits and these fungi pose a potential serious risk to wooden cultural heritage objects along the coastline of Western Greenland since they can cause extensive decay and destroy the wood.
It cannot be excluded that the observed Basidiomycota DNA originate from dead fungi and that the observed decay took place a long time ago when the wood was still exposed on the surface. With time and sustained deposition, the wood was buried and exposed to an environment with less oxygen and eventually the Basidiomycota fungi were outcompeted by the Ascomycota fungi. We found a notably higher average amount of Basidiomycota DNA in our replicates of natural grown dead Salix and exposed historical wood than in the archaeological wood from the active soil layer of the archaeological deposit (both buried and exposed). This suggests that the archaeological wood is more protected against Basidiomycota than wood exposed directly to the soil surface. Ascomycetes are known to tolerate higher moisture and lower oxygen levels than Basidiomycetes24. However, the exposed historical samples were only dominated by brown rot decay in one of the six samples; the five other samples were dominated by soft rot. Interestingly, the exposed archaeological samples did not have a higher average amount of Basidiomycota DNA than the buried archaeological samples, and the visual analysis showed very similar decay patterns between the two types of archaeological wood material. This indicates that despite exposure to ambient air and thereby less protection from the soil (lower oxygen level and more moisture), the wood is not in a markedly worse state of preservation than wood buried within the active layer.
Basidiomycota are aggressive fungi that may outcompete slower growing soft rot Ascomycota and therefore tend to become the dominant degrader when environmental conditions are conducive for their growth32. In this study, however, only very few wood samples were dominated by brown rot decay or by advanced CML decay that could originate from simultaneous white rot decay. Despite the fact that Basidiomycota are present at all the examined sites, Ascomycota seems to be better adapted to the conditions found within the archaeological deposits. Although the investigated samples were collected from sites that are exposed to different climatic conditions (Table 1), the results show no geographical trends in relation to both the severity of soft rot and the distribution of Basidiomycota wood degrading fungi. Therefore, it is not possible to pin-point the dominating driver for soft rot decay or the dominating limiting factor for Basidiomycota growth e.g. temperature, oxygen availability, nutrients, length of growth season, annual sum of thawing degree days or precipitation rates. This is probably because the fungal species interactions and fungal life cycles are a result of more complex dependence of several of these factors.
Air temperatures in Greenland are expected to increase with up to 5 °C within the next century33 leading to a marked increase in soil temperatures7 and a significant loss of permafrost34. We did not include archaeological wood samples from the permafrost layer in this investigation, but previous studies have shown that permafrost provides close to optimal preservation conditions for wooden artefacts6. Our results suggest that permafrost melt and exposure to the less stabile conditions within the active layer will likely lead to severe soft rot decay. It is not possible to estimate the rate at which the soft rot in our samples has occurred. However, previous incubation studies have shown that the degradation of wood is highly temperature dependent with rates increasing exponentially with increasing temperature6. The presence of early stages soft rot may not be critical, but advanced soft rot degradation results in a very fragile wood structure and little strength remaining in the wood. This means that the many wooden artefacts currently buried within the active layer may collapse and lose their scientific value if not kept moist. Thereby, wooden artefacts buried in areas where summer precipitation rates decrease, or the duration and frequency of drought periods increase may be especially vulnerable.
Furthermore, given the presence of Basidiomycota at all sites examined in this study, there is a potential risk that as environmental conditions change these fungi will become more active within the archaeological deposits causing serious losses in the future.
In this study, we found that Ascomycota soft rot fungi were the dominant decay organisms at all 11 sites studied regardless of climate and local environment. Ascomycota had caused extensive soft rot decay in a high number of samples leaving a weak and vulnerable wood structure, however with preserved wood anatomical features. This first and foremost suggest that large parts of the wooden artefacts that are currently buried within the active layers of Greenland’s archaeological deposits may collapse and lose their scientific value if not kept moist. Secondly, it indicates that the uniquely well-preserved wooden artefacts that are currently buried within the permafrost will be attacked by Ascomycota and be subject to soft rot decay when the permafrost melts. However, the rate at which the soft rot decay occurs is still largely unknown. Basidiomycota was also present at all 11 sites but appeared to be outcompeted by Ascomycota in the great majority of the samples. Basidiomycota decay destroys the anatomical features of the wood and thereby the scientific value of the artefacts. Currently, multiple factors are most likely limiting the growth of the Basidiomycota wood degraders, but if growth conditions become more favourable with a changing environment, the archaeological wooden artefacts could be further endangered by these more aggressive wood destroying fungi.
Sampling of wood material
In total 45 buried archaeological samples, 21 exposed archaeological samples, six exposed historical samples, and 12 native dead Salix glauca samples were collected during field campaigns in the years 2009 and 2012–2016. Samples collected in 2014–2016 were placed in sterile bags and kept below freezing during transport and storage until investigated in the laboratory. Samples collected in 2009, 2012 and 2013 were collected in normal PE sample bags and kept frozen until investigated in the laboratory (Supplementary Table S1).
Mean annual precipitation was computed based on snow and rain-fall in mm water equivalents from the regional climate model MAR v3.835, using daily 5 km data. The climate model handles redistribution of the winter snowpack through the CROCUS modelling scheme. Surface temperatures were extracted from the satellite based MOD11A1 MODIS product, offering daily data at 1 km spatial resolution. This product was corrected for cloud cover36 to minimize a cold winter bias, and subsequently gap-filled with MAR v3.8 surface temperatures again at 5 km spatial resolution. Only MAR-data from terrestrial areas were used; sea- and glacier-classified areas were omitted.
Visual decay analysis and wood identification
66 archaeological (buried and exposed) and six historical wood samples from 11 archaeological sites were visually inspected to determine if the wood surface was intact or decayed. Thin sections, hand cut with razorblade in transverse, radial and tangential longitudinal direction stained with 0.1% (w/w) safranin O (Sigma-Aldrich, MO, USA), were examined in bright field transmission microscope (Leica DM5000B). Wood genera were determined using anatomical characteristics37. Decay types were determined by examination of anatomical characteristics for the major types of wood decaying microorganisms24,25,32,38,39. The degree of degradation was determined by examination of cross section from surface to inner xylem and an average degree of degradation of each sample was categorised either as intact, partly decayed, or in an advanced stage of decay (Supplementary Methods S10 online). Nine buried archaeological samples were selected for further scanning electron microscopy analysis (Supplementary Methods S10 online).
Culturing and identification of fungi
Five sub-samples approximately 1 × 1 × 0.5 mm3 were cut as biological replicas from 53 archaeological samples (buried or exposed), the six exposed historical wood samples, and 12 native dead exposed Salix glauca samples using aseptic techniques. The sub-samples were incubated on at least two and up to five different culture media including at least one Basidiomycota specific culture media (Supplementary Methods S10 online). The sub-samples were incubated at room temperature (20–22 °C) as previous studies have shown that filamentous fungi from polar environments are primarily psychrotrophs or mesotrophs and can grow above 20°C11,12,20. Pure cultures were obtained by transferring isolations to individual plates of malt extract agar (15.0 g malt extract and 15.0 g agar in 1L deionized water). DNA was extracted from pure culture plates using a CTAB extraction protocol and the internal transcribed spacer region (ITS) of rDNA was amplified, sequenced and processed for GenBank searches following that of Blanchette et al.14.
Quantitative Basidiomycota DNA determination
Material preparation: Three biological replicas of approximately 100 mg were taken from 46 archaeological samples (buried or exposed), six exposed historical wood samples, and 12 native dead exposed Salix glauca samples with aseptic and DNA-clean (10% bleach) techniques preferably in three different areas with visual surface decay. Each sub-sample was taken 1–3 mm below the surface (to avoid surface contamination) and cut as fine as possible with a scalpel. Two Basidiomycota monocultures, Rhodonia placenta ((Fr.) Niemelä, K.H. Larss. & Schigel (syn. Postia placenta) strain FPRL 280 and Trametes versicolor ((L.) Lloyd) stain CTB 863 were grown aseptic on cellophane to produce mycelia for a standard curve. One PCR-clean steal ball was added to each 2 mL Eppendorf tube (with Araldite glue in the lid) and the samples were ball milled 2 min (Retsch MM300, Retsch Gmbh, Haan, Germany) at speed 30 (1/s) after instant freezing of tubes and the mill sample holders in liquid nitrogen. 40 wood subsamples were too wet to mill directly and were freeze dried (Heto Drywinner, DW 1, 0-60E) prior to milling.
DNA extraction: Using 40.0 mg aliquots of powdered wood material genomic DNA was isolated from each subsample with the DNeasy Plant Mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. 1.00 ng pGEM of an internal reference DNA composed of pGEM plasmid (pGEM-3Z Vector, Promega, Madison, WI, USA) was added to each subsample upon DNA isolation to normalize for the natural variation in extractability of DNA in wood40. The extracted DNA was eluted in 50 µL AE buffer. The quantity and quality of the extracted DNA was determined by analysing 2 µL eluted DNA with a UV–VIS micro volume spectrophotometer (NanoDrop 2000, Thermo Scientific, Waltham, MA, USA). Details about experimental sample dilution and preparation of standard curves are provided in Supplementary Methods S10 online.
qPCR conditions: The quantity of Basidiomycota DNA (DNAbas) in each subsample was determined by quantitative polymerase chain reaction (qPCR). Amplifications of DNAbas were performed in Fast SYBRGreen Master Mix (Applied Biosystems, Waltham, MA, USA) whereas pGEM was amplified in TaqMan Universal PCR Master Mix (Applied Biosystems, Waltham, MA, USA). The qPCR was performed with AB Applied Biosystems ViiA 7 (Applied Biosystems, Waltham, MA, USA) running with an initial activation step at 95 °C for 2 min followed by 45 PCR cycles running at 95 °C for 15 s followed by 1 min at 60 °C. A melting curve was determined at the end of cycling DNAbas to get information on the quality of the PCR products in each sample. Ct values were determined with the Applied Biosystems ViiA 7, v1.2.1 software supplied with the instrument. The extent of amplification was calculated as a mean Cq value of two technical replicates for each sample and calculated into gDNA concentration via gDNA and pGEM standard curves.
Identification of Basidiomycota in gDNA extracted directly from wood samples
The melting curves from the quantitative Basidiomycota gDNA determination revealed that 39 subsamples from 21 different wood samples had a high-quality melting curve with a single PCR product. The DNA extractions from these samples were used for sequencing for fungal species identification. Amplification was performed with the same 5.8sr forward primer and ITS4-X reverse primer as used for quantitative Basidiomycota DNA determination. Details about the mastermix is provided in Supplementary Methods S10 online. The DNA extractions from the selected subsamples were diluted 100 × except for three samples that were diluted 1,000 × . Each subsample was prepared by mixing 2.00 µL diluted isolated gDNA with the mastermix prior to amplification. In addition one blank sample was prepared by mixing 2.00 µL nuclease free water with the mastermix. Details about PCR amplification is provided in Supplementary Methods S10 online. 32 of the 39 run PCR products showed a band at the agarose gel and were selected for Sanger sequencing run at GATC Biotech (Konstanz, Germany). 20 of the sequenced PCR products were De Novo assembled using Geneious 7.0 (Biomatters, Auckland, New Zealand) to a consensus sequence from the forward and reverse sequence and the best nucleotide BLAST (U.S. National Library of Medicine) matched to GenBank sequences using, if possible, accessions from taxonomic publications was performed. PCR products from five subsamples could not be De Novo assembled but nucleotide BLAST on both the forward and reverse sequence gave match of the same genus. PCR products from five other subsamples gave a high quality forward or reverse sequence that could be BLAST but non or very low quality of the opposite sequence. PCR products from two subsamples did not have a high enough sequence quality for either De Novo assembling or nucleotide BLAST on either the forward or reverse sequence.
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
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We gratefully acknowledge financial support from the Carlsberg Foundation (CF14-0454 and CF15-0112) and VELUX FONDEN (33813). Special thanks to Hans Harmsen and Christian Koch Madsen from Nunatta Katersugaasivia Allagaateqarfialu (Greenland National Museum & Archives) for fieldwork and export permission, to the NOW Project for sample collection in Nuulliit, to Sigrun Kolstad and Inger Heldal from NIBIO for laboratory support, and to research professor Carl Gunnar Fossdal from NIBIO for help with troubleshooting.
The authors declare no competing interests.
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Pedersen, N.B., Matthiesen, H., Blanchette, R.A. et al. Fungal attack on archaeological wooden artefacts in the Arctic—implications in a changing climate. Sci Rep 10, 14577 (2020). https://doi.org/10.1038/s41598-020-71518-5