Correction to: Scientific Reports https://doi.org/10.1038/s41598-020-65355-9, published online 20 May 2020
This Article contains errors in the Methods section, under the subheading ‘Generation of Cd103−/− mice’,
“The linearized targeting construct was introduced by electroporation into C57BL/6-derived JN/2 recombinant embryonic stem cell (ESC) and neomycin-resistant clones were first screened for homologous recombination by PCR utilizing a pair of the following oligonucleotides: Primer 1 (5′-ATA TGT AGT GTC TGG TCA GGA TAA TAG TTG-3′) and Primer 2 (5′-ATA ACC TCC TCT CCT ATG GTA CCT AAA C-3′).”
should read:
“The linearized targeting construct was introduced by electroporation into C57BL/6-derived JN/2 recombinant embryonic stem cell (ESC) and neomycin-resistant clones were first screened for homologous recombination by PCR utilizing a pair of the following oligonucleotides: Primer 1 (5′-ATA TGT AGT GTC TGG TCA GGA TAA TAG TTG-3′) and Primer 3 (5′-ATA ACC TCC TCT CCT ATG GTA CCT AAA C-3′).”
“Transmission of the targeted allele was confirmed by PCR with Primer 1 and Primer 3 (5′-CTT TAT ATT TCA TTT TTG CTC AGG CTT C-3′). The mutant mice were cross-mated for more than nine generations with B6.FLIP mice to excise the flanked FRT sites by Flp-recombinase, and 8- to 12-week-old Cd103+/+ littermates were used as WT mice. Then, Cd103+/− littermates were crossed to obtain homozygotes, and transmission of the targeted allele was confirmed by PCR with Primer 1 and Primer 3.”
should read:
“Transmission of the targeted allele was confirmed by PCR with Primer 1 and Primer 2 (5′-CTT TAT ATT TCA TTT TTG CTC AGG CTT C-3′). The mutant mice were cross-mated for more than nine generations with B6.FLIP mice to excise the flanked FRT sites by Flp-recombinase, and 8- to 12-week-old Cd103+/+ littermates were used as WT mice. Then, Cd103+/− littermates were crossed to obtain homozygotes, and transmission of the targeted allele was confirmed by PCR with Primer 1 and Primer 2.”
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Fukui, T., Fukaya, T., Uto, T. et al. Author Correction: Pivotal role of CD103 in the development of psoriasiform dermatitis. Sci Rep 10, 16375 (2020). https://doi.org/10.1038/s41598-020-71156-x
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DOI: https://doi.org/10.1038/s41598-020-71156-x
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