The differential associations of adipose depots with metabolic risk during obesity have been proposed to be controlled by environmental and genetic factors. We evaluated the regional differences in transcriptome signatures between abdominal (aSAT) and gluteal subcutaneous adipose tissue (gSAT) in obese black South African women and tested the hypothesis that 12-week exercise training alters gene expression patterns in a depot-specific manner. Twelve young women performed 12-weeks of supervised aerobic and resistance training. Pre- and post-intervention measurements included peak oxygen consumption (VO2peak), whole-body composition and unbiased gene expression analysis of SAT depots. VO2peak increased, body weight decreased, and body fat distribution improved with exercise training (p < 0.05). The expression of 15 genes, mainly associated with embryonic development, differed between SAT depots at baseline, whereas 318 genes were differentially expressed post-training (p < 0.05). Four developmental genes were differentially expressed between these depots at both time points (HOXA5, DMRT2, DMRT3 and CSN1S1). Exercise training induced changes in the expression of genes associated with immune and inflammatory responses, and lipid metabolism in gSAT, and muscle-associated processes in aSAT. This study showed differences in developmental processes regulating SAT distribution and expandability of distinct depots, and depot-specific adaptation to exercise training in black South African women with obesity.
Body fat distribution is an independent contributor to the development of obesity-associated metabolic diseases1,2. While central fat accumulation (both in visceral and abdominal subcutaneous areas) has been associated with greater risk for developing metabolic diseases, lower-body fat (gluteo-femoral) appears to have positive effects on metabolic health3,4,5. The mechanisms underlying these depot-specific associations with metabolic risks are not fully understood. However, environmental and genetic factors could mediate differences in gene expression, metabolism and function between fat depots6,7.
Adipose tissue (AT) accumulation follows a specific individual pattern during positive energy balance, with the preferential expansion of subcutaneous vs. visceral depot, largely determined by heritable factors1. This specific response to excess calories could also be influenced by site-specific sets of developmental genes8,9. Numerous studies have reported an extensive number of differentially expressed genes (DEGs) between visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) (both abdominal and gluteal)4,8,10,11. This suggests different developmental lineage, potentially explaining depot-specific adipose metabolism and association with metabolic risk. However, whether the difference in embryonic origin exists within SAT depots remains elusive. Indeed, in addition to their functional differences and their distinctive associations with metabolic risk, epigenetic differences between abdominal SAT (aSAT) and gluteal SAT (gSAT) have been shown5,8,12,13. Notably, these findings were mainly derived from studies in European populations and no data is available on the depot-specific SAT transcriptome profile in an exclusively African population, highlighting the need to study SAT signatures in Africans.
Sub-Saharan Africa is experiencing exponential growth in the prevalence of obesity with more women (15%) affected than men (6%)14. South Africa presents with the highest prevalence of overweight and obesity in women (40%) in this region15, along with a high incidence of type 2 diabetes (14.7%)16. Moreover, despite African women having more gluteo-femoral fat, and relatively less VAT17,18, they are more insulin resistant than their white (European ancestry) counterparts for the same level of body mass index (BMI)19. Furthermore, gSAT in black African women is characterized by higher expression of genes linked to hypoxia, fibrosis and inflammation than that of their white counterparts20. This suggests that the gene expression profile of SAT depots is ethnic-specific and consequently, might have divergent metabolic effects in black African compared to European women.
Excessive fat accumulation consecutive to chronic positive energy balance is sustained by low physical activity21. Exercise training (one of the principal components of physical activity) can exert beneficial effects on metabolic health via modifications in AT metabolism22. Specifically, previous studies have shown changes in AT lipid metabolism (lipolysis, lipogenesis), fatty acid uptake and oxidation, as well as glucose homeostasis and mitochondrial activity after exercise training in individuals with obesity23,24. However, the extent of these changes on SAT transcriptome has not yet been investigated in African populations.
Using an unbiased microarray analysis approach, this study aimed to evaluate the regional differences in gene expression profiles between aSAT and gSAT in obese black South African women and tested the hypothesis that the gene expression pattern is altered in a depot-specific manner in response to 12-weeks of combined aerobic and resistance exercise training.
The basic characteristics of the participants involved in this study are presented in Table 1. These premenopausal women were on average 23 ± 3 years of age with a BMI of 33.8 ± 2.6 kg/m². They were centrally obese, with waist circumference (WC) of 102 (±5) cm and significantly more subcutaneous (5451 ± 803 cm3) than visceral fat (971 ± 330 cm3) (Table 1). In response to exercise training, cardiorespiratory fitness (VO2peak) increased (p < 0.05), and weight, BMI, waist circumference, waist/hip ratio (WHR) decreased. While fat-free soft tissue mass (FFSTM) and total body fat (%) did not change, both android and gynoid fat mass (%) decreased (p < 0.05), and there was a tendency for VAT and SAT volumes to decrease (p < 0.1). There was no significant difference in daily energy or macronutrient intake after the 12-week exercise training intervention (Supplementary Table S1).
Gene expression differences between SAT depots at baseline
The differences in gene expression profiles between aSAT and gSAT at baseline and after exercise training are presented in Fig. 1A,B, respectively, and the multidimensional scaling (MDS) plots are shown in Supplementary Figure 3. At baseline, only 15 genes were differentially expressed between aSAT and gSAT (|log2 fold change (LFC) | ≥ 0.58; p ≤ 0.05) with the expression of 13 genes being higher and 2 genes being lower in aSAT vs. gSAT (Fig. 1A; Table 2). Given this small number of DEGs, the motif enrichment analysis was not reliable, preventing us from reporting on putative transcription factor binding sites (TFBS) at baseline (see supplementary file).
Gene expression differences between SAT depots after 12-week exercise training
In contrast to the small number of DEGs between fat depots at baseline, we identified 318 DEGs after exercise training (Fig. 1B); with the expression of 166 genes being higher and 152 genes being lower in aSAT vs gSAT (Table 3 for the top 10 DEGs; Supplementary Table S2). Notably, 11 of these genes overlapped between the SAT depots before and after exercise training (Fig. 1C). The most prominent genes distinguishing the SAT depots at both time points (highlighted in bold in Tables 2 and 3) were doublesex and mab-3 related transcription factor (DMRT)2, DMRT3, and homeobox (HOX) A5, with higher expression levels in aSAT compared to gSAT; as well as casein alpha s1 (CSN1S1), with lower expression in aSAT vs gSAT. Several HOX genes were found with higher expression levels in aSAT than gSAT at baseline or after exercise training (HOXA3, HOXA5, HOXA9, HOXB5, HOXB8, HOPX, iroquois homeobox (IRX) 2 and IRX5). The evaluation of the biological processes represented by the DEGs between the depots at both time points using STRING showed that they were mainly enriched for gene ontology (GO) terms: embryonic development, anatomical structure and developmental processes at baseline (Fig. 2), and more diverse functional-related processes at post-training (Supplementary Fig. 1). In addition, motif enrichment analyses using hypergeometric optimization of motif enrichment (HOMER) were completed and a detailed report is provided in the supplementary document. Briefly, we identified known and/or de novo enriched motifs for higher and lower DEGs in aSAT vs gSAT. In the higher DEGs in aSAT vs. gSAT, the predicted enriched motifs were NF1 half-site and LRF binding motif (associated with adipogenesis and glycolysis), with no enriched de novo motifs. In contrast, in the lower DEGs in aSAT vs gSAT we identified an enrichment of RUNX binding site (associated with the immune system and pro-inflammation; see supplementary document), USF1 binding site, and E-box promoter element (which can act as a USF1 binding site25. The transcription factor USF1 is linked to familial combined hyperlipidemia and has been associated with the metabolism of brown adipose tissue in mice26. Furthermore, we identified de novo enriched motifs for lower DEGs in aSAT vs gSAT involved in adipocyte differentiation (ZNF467)27 and to immune system response in humans (PRDM1)28.
Gene expression in gluteal SAT in response to exercise training
To evaluate the depot-specific response to exercise training, we compared the gene expression level in gSAT (Fig. 3A) and aSAT (Fig. 3B) pre and post-training, respectively. In gSAT, we identified 61 DEGs (|LFC | ≥ 0.58; p ≤ 0.05) with 54 genes upregulated and 7 genes downregulated in response to exercise training (Table 4; Supplementary Table S3). The biological processes most affected by exercise training (Fig. 4) were immune and inflammatory responses (e.g. SPP1, CCL22, MMP9, CHI3L1, HP), regulation of lipid metabolism (e.g. APOE, PLTP, SPP1, C3, APOC1, SREBF1) and regulation of plasma lipoprotein particle levels and remodelling (e.g. APOC1, APOE, PLA2G7, PLTP, LIPA). Moreover, the HOMER analysis on the combined set of up- and down-regulated genes (due to the low number of downregulated genes) showed enrichment for the transcription factor complex AP-1 similar to binding motifs of Fos-related factors or Jun family. Additionally, two known predicted binding motifs: activation transcription factor 3 (Atf3) and basic leucine zipper ATF-like transcription factor (BATF) were identified (see supplementary document). Atf3 belongs to the cAMP-responsive element-binding (CREB) class and interacts with Jun-related factors, and BATF mediates the dimerization of Fos and Jun factors to form the AP-1 transcription factor complex. The AP-1 transcription factor complex is a regulator of cell proliferation, differentiation and transformation in response to stimuli like cytokines, stress or growth factor29.
Gene expression in abdominal SAT in response to exercise training
Within aSAT, the expression of 77 genes changed in response to exercise training (|LFC | ≥ 0.58; p ≤ 0.05), with 55 genes upregulated and 22 genes downregulated (Table 5; Supplementary Table S4). Surprisingly, these genes mostly represented muscle-associated processes (e.g. ACTA1, MYH7, MYL2, TCAP, CKM) and immune response (e.g. HP, COL1A1, CD3D, IL7R) (Fig. 5). Notably, only 3 genes had commonly altered expression levels in aSAT and gSAT after exercise training (COL1A1, HP, CILP) (Fig. 3C). We subsequently combined the up- and down-regulated genes in aSAT in response to exercise training to identify enriched motifs, as the number of downregulated genes was only 22. Four known TFBS were found in the promoter regions of the DEGs (CarG-box DNA motif, Mef2b RUNX1 and Gfi1b). Further, Smad2 was the best match of the predicted de novo motifs, which is a regulator of multiple cellular processes, such as cell proliferation, apoptosis and differentiation (see supplementary document).
Differential mRNA expression in adipocytes and cells of the stromal vascular fraction
To assess the cellular origin of the whole-SAT mRNA expression of HOXA5, DMRT2, DMRT3, R-Spondin 3 (RSPO3), CS1N1 and matrix metallopeptidase 9 (MMP9), mRNA levels of these genes were measured in adipocytes and the stromal vascular fraction (SVF) of an independent cohort (n = 29) detailed in the Method section. While RSPO3 and CS1N1 mRNA levels were higher in adipocytes compared to SVF (p < 0.001), MMP9 mRNA level was higher in SVF than in adipocytes (p < 0.0001) (Supplementary Fig. 2). In addition, there was a trend towards a higher expression of DMRT3 (p = 0.065) and HOXA5 (p = 0.075) in adipocytes compared to SVF, but DMRT2 expression was not different between these cell fractions (p = 0.178) (Supplementary Fig. 2).
This is the first study to our knowledge that has investigated the regional differences in SAT transcriptome signatures in obese African women and showed differences in gene expression profiles between aSAT and gSAT at baseline and in response to exercise training. As a key result of our unbiased analysis, we found 15 DEGs between these fat depots at baseline, which were mainly associated with embryonic development (e.g. HOXA5, DMRT2, HOXB8, IRX5, IRX2) and regulation of anatomical structure morphogenesis (e.g. DMRT2, DMRT3, HOXA5, RSPO3). Depot-specific AT transcriptome signatures were strongly pronounced in response to the 12-weeks structured exercise training program, such that 318 genes were differentially expressed between the depots, and only three genes commonly changed in aSAT and gSAT. Interestingly, we identified four developmental genes as the most prominent DEGs between SAT depots (DMRT2, DMRT3 and HOXA5 higher, and CSN1S1 lower expressed in aSAT compared to gSAT) that were unaffected by exercise training.
Adipose tissue depots have been proposed to arise from different mesodermal layers during embryonic development, directed through epigenetic-programmed mechanisms8,30. HOX family is among the most studied families of developmental genes in AT and has been widely identified as fundamental in controlling the body plan along the anterior-posterior axis in diverse species including humans8. HOX genes have also been suggested as determinants of early regional differentiation by directing the evolution and differentiation from mesoderm layers to different AT depots12. We have previously shown in both mice and humans (Europeans ancestry) that multiple HOX genes are involved in embryonic development and pattern specification (e.g. HOXA5, HOXC8, HOXC9), and play an important role in obesity and body fat distribution, with potential functional differences between distinct AT depots9. However, to our knowledge, no studies have explored HOX genes expression in an African population that may explain their distinct body fat distribution. Moreover, there is a lack of data on the depot-specific SAT transcriptome profile in an exclusively African population, highlighting the need to study SAT signatures in Africans and the novelty of the present study. We show that the following HOX genes were more highly expressed in aSAT than in gSAT: HOXA5 (at both time points), IRX2, HOXB8 and IRX5 (at baseline), and IRX2, HOPX, HOXA3, HOXB5 and HOXA9 (after exercise training). Higher HOXA5 expression in aSAT compared to gSAT has been previously shown in obese men and women of European ancestry, as well as higher expression of this gene and other HOX genes in the SVF of aSAT compared to that of gSAT12. Furthermore, high levels of HOXA5 have been closely related to BMI and body fat distribution in both obese humans and animal models6,9,12,31,32. In addition, several homeobox genes were showed to be upregulated in aSAT and VAT (e.g. HOXA3, HOXA9, HOXB5, HOXB8, IRX5, IRX2 and HOPX)5,7,9,12,13,33. This suggests a similar developmental origin of aSAT and VAT, rather than aSAT and gSAT8. Notably, IRX genes are potentially involved in the mechanisms underlying the genetic association between FTO and obesity34. The causal variant (rs1421085) of this association eliminates IRX3 and IRX5 repression, leading to a cell-autonomous shift from browning and thermogenesis of white adipocytes to lipid storage, increased fat stores and body-weight gain34. Higher expression of IRX genes in aSAT could therefore be one of the causal factors in the detrimental central fat accumulation (aSAT) in obese individuals. However, this should be further investigated in a cohort of African women characterized by low central and high gynoid fat accumulation.
Unlike the homeobox family, DMRT family has been less studied in the context of obesity and body fat distribution. Mostly involved in sex differentiation and gonadal development during embryogenesis in a wide range of species35,36,37, the expression of DMRT genes has also been reported in aSAT of individuals with obesity37. In the present study, we found higher expression of DMRT2 and DMRT3 in aSAT vs gSAT at baseline and post-exercise training, similar to findings from Passaro et al. in healthy men at rest8. Additionally, a recent study has identified DMRT3 expression in omental vs aSAT as a novel marker for the development of insulin resistance38. Therefore, DMRT2 and DMRT3 might be novel candidate genes involved in “unhealthy” central fat accumulation. Interestingly, we showed that these genes are expressed both in adipocytes and cells of the SVF, although a trend for higher expression for DMRT3 and HOXA5 in adipocytes than cells of the SVF was observed. Therefore, both cell types participate in the differential gene expression between the SAT depots, especially with regards to the expression of developmental genes. However, the functional role of these genes in AT development and function remains to be elucidated.
In contrast, we found higher expression of the gene CSN1S1 in gSAT compared to aSAT at baseline and post-exercise training. Previously known as a functionally unreported adipose-specific gene, CSN1S1 expression was recently shown to be significantly more highly expressed in SAT (abdominal) of obese compared to lean individuals39. Moreover, this gene is downregulated in aSAT after weight loss as shown in morbidly obese individuals40. Increased CSN1S1 is involved in immune/inflammatory responses41 and may also play a key role in adipogenesis and lipid metabolism39. In the present study, the expression of CSN1S1 was significantly higher in adipocytes than cells of the SVF in the aSAT of our independent cohort. These findings corroborate evidence showing higher expression of CSN1S1 in mature adipocytes compared to preadipocytes and increased expression during adipose-derived stem cell differentiation39. Therefore, higher expression of CSN1S1 might be reflective of higher storage capacity in gSAT compared to aSAT.
Taken together, the unique gene expression signatures of aSAT and gSAT suggest differences in developmental processes regulating AT distribution and expandability of distinct depots. Our data add to the notion that there are intrinsic morphological and functional differences between central and lower-body fat5,6,12. Interestingly, from the comparison of these depots at baseline and after exercise training, 18 of the DEGs were overlapping and were enriched for anatomical structure morphogenesis. We therefore postulate that the expression of developmental genes is not affected by external/environmental stimuli (such as exercise training) once they have been set during the early life stage. Rather, these differences are maintained across the depots as previously shown in cell culture studies9,12,13,42. Moreover, developmental genes might be actively involved in AT function, distribution and remodelling43. Genome-wide association studies and meta-analysis on European individuals identified several genes within the WHR-associated loci with differential transcription between aSAT and gSAT44. However, except for RSPO3, the DEGs emerging from the present study did not overlap with these previous reports44,45, most probably explained by the different ethnicities.
Given the lower number of DEGs between SAT depots at baseline (15 genes) compared to the number after exercise training (318 genes), we propose that the major depot-specific differences reflect the heterogeneous capacity of SAT to adapt to behavioural (or environmental) changes, such as exercise training. This hypothesis is supported by a recent study showing that fat depots (e.g. SAT, VAT, liver, perirenal) respond with a highly variable alteration in fat mass during a weight loss intervention (dietary and physical activity)46. The depot-specific AT response to exercise training could provide insight into metabolic and signalling pathways implicating fat distribution in the development of metabolic disorders. We therefore investigated the specific response of each SAT depot to exercise training and found expression changes in different sets of genes and associated biological processes. Notably, the expression levels of only three genes commonly changed in both depots. This suggests differential regulatory mechanisms involved in cellular and endocrine function. Specifically, the DEGs in gSAT were mostly enriched for immune and inflammatory responses (driven by upregulated genes), suggesting increased inflammatory processes after exercise training as recently shown in this cohort47. This is further supported by our finding of motif analyses, showing that the enriched transcription factors were associated with immune system response and pro-inflammation for higher DEGs in gSAT vs aSAT after exercise training. Exercise training can activate macrophage production of cytokines, leading to the modulation of monocyte chemotaxis, differentiation into macrophages and activation48. In addition, adipocytes readily respond to exercise training by modulating the transcriptomic response of pro-inflammatory genes48. Therefore, an increased inflammatory response is likely to originate both from adipocytes and AT immune cell signalling. In line with this, both cell types can display similar transcriptomic profiles especially when the macrophages engulf lipids49.
The increased inflammatory response seems to be contradictory, given the extensively reported association between AT inflammation and the development of metabolic diseases in obese humans and mouse models49,50,51 and decreased white AT inflammation after exercise training52. However, elevated immune response and activation of inflammatory pathways have been shown in SAT after acute exercise sessions48,53; and no studies have investigated the specific adaptations in gSAT in response to a long-term exercise training. The upregulation of inflammatory-related response could reflect structural changes of AT and adaptations in the early phase of the training, which is downregulated once structural changes are established48. However, the beneficial effect of exercise training on AT physiology is the product of continuous changes induced by repeated transient acute exercise bouts54. Hence the observed upregulation of immune and inflammatory responses after the present exercise training program mirrors a cumulative response of single exercise bouts. Notably, the sample analyzed in the present study were collected at least 48–72 hours after the last exercise training session to exclude the potential acute effects of the last bout of exercise training. Furthermore, these women have never completed structured exercise training before participating in this program, supporting our hypothesis that sustained inflammatory activation after 12-week exercise training is associated with AT reorganization and remodeling47. Consistently, the identified DEGs in gSAT in response to this combined aerobic and resistance exercise training intervention were also involved in biological processes regulating lipid metabolism and plasma lipoprotein particle levels and remodelling (e.g. MMP9, SPP1, APOC1, ITGAX and PLA2G7). Further, these DEGs were also enriched for transcription factors complex (AP-1) regulating cell proliferation, differentiation and transformation in response to cytokine production. Changes in metabolic homeostasis such as improved body composition or reduced adiposity can trigger immune and inflammatory processes53. For instance, the stimulation of lipolysis and enhanced fat mobilization in AT after exercise training was shown to be promoted by enhanced production of pro-inflammatory cytokines, such as IL-655. Furthermore, pro-inflammatory signalling in adipocytes mediates angiogenesis and extracellular matrix (ECM) remodelling56,57 and the overall reduction of stored triglycerides in response to exercise training have been shown to precede an extensive remodelling of AT58,59. In the present study, we showed a higher expression of MMP9 in the cells from the SVF compared to adipocytes in our independent cohort of European men and women with obesity. Therefore, higher expression of MMP9 in response to exercise training may reflect adaptations of cells within the SVF rather than an adipocytes-specific response. This is in agreement with the hypothesis of ECM modification in gSAT after exercise training, indicative of gynoid fat remodeling47.
Surprisingly, in aSAT most of the DEGs were enriched for muscle-associated processes (e.g. ACAT1, TCAP, MYL2, MYH7, MYBPC). Similar muscle-related gene expression profiles were found for the first time in brown adipose of high fat diet-induced obesity-resistant rats (e.g. TNNT3, ACTA1, ACTN3, MYLPF), but their functional role in AT has not been clarified60. As whole AT was used in the present study, we cannot exclude the possibility that these genes were expressed from non-adipocytes/non-immune cells. Subsequent investigations on isolated cell fractions, or single-cell RNA sequencing approaches, as well as cell culture experiments, are required to further elucidate the mechanistic link between exercise training and signature changes of AT.
Our analyses were limited by the amount of material we could obtain from the SAT biopsies. Histological, as well as extensive proteome analyses, could not be performed and we can therefore not conclude whether gene expression differences were translated at the protein level. Moreover, the lack of a normal-weight control as a comparison group prevented us from concluding whether our findings are relevant pathways related to obesity and exercise training rather than an adaptation to the obese state. As the detection of DEGs was based on statistical methods, further validation is needed to rule out the possibility of false positives. Due to the small sample of women included in this study, the data may not be representative of African women and cannot be directly translated to other groups or individuals with different phenotypes and/or age, gender, socioeconomic and/or health status. Nevertheless, records on AT transcriptome of African populations are rare and this is one of the first studies providing unique data on SAT depot-specific gene expression profiles and adaptation to exercise training in black African women with obesity. This research work generated hypotheses about novel candidate genes potentially implicated in the relationship between body fat distribution, AT adaptation and metabolic status. Further investigations in more representative samples of black Africans, including normal-weight groups and men are needed to validate these findings.
In conclusion, the current study demonstrates differential gene expression profiles of SAT depots (gluteal and abdominal) in African women with obesity. While the differences at baseline suggest dissimilar developmental origin, the exercise training intervention unmasked a heterogeneous response of different SAT depots to an external stimulus. The specific functional and metabolic responses could be related to intrinsic differences in the expression of developmental genes set at an early-life stage in each AT depot. These were represented by an upregulation of the immune response and inflammatory processes, lipid metabolism and tissue remodelling in gSAT and surprisingly, mainly muscle-related processes in aSAT. Importantly, we identified four genes (CSN1S1, DMRT2, DMRT3 and HOXA5) as novel candidate genes of body fat distribution pattern in Africans, whose biological function and implication in the pathogenesis of obesity-associated metabolic diseases remain to be unravelled.
This is a sub-study from a previously reported study using exercise training as a model to investigate the mechanisms underlying insulin resistance in a cohort of black South African women61. The general methods have been extensively reported61 and are only briefly described here. The study protocol was designed in accordance with the principles of the Helsinki Declaration with approval from the Human Research Ethics Committee of University of Cape Town (HREC REF: 827/2016) and registered to the Pan African Clinical Trial Registry (trial registration: PACTR201711002789113). Participants signed a written informed consent before enrollment in this study. They were included if both their parents were of isiXhosa (black African) ancestry, if they had a stable weight (<5% change from maximum weight for the last 6 months), no known metabolic or inflammatory diseases (e.g. HIV tuberculosis, active hepatitis, or rheumatoid arthritis). They were not taking any medications, non-smokers, not pregnant or lactating and were using injectable contraception (depot medroxyprogesterone acetate, 400 mg).
Participants’ selection and Intervention
Twelve of the 20 participants from the parent study were included in this study. The selection was based on the greatest reduction in body weight in response to the exercise training intervention, as well as sample availability. Although weight loss was not the main outcome of that study, the participants with the greatest weight loss were specifically selected to maximize the responses observed in the SAT depots to exercise training. The women performed 12 weeks of supervised exercise training consisting of combined aerobic and resistance training (60–70% heart rate; HRpeak) progressing from 40 to 60 min, 4 days per week by a trained facilitator. The exercise training consisted of cardiovascular exercises in the form of aerobic dance, running, skipping, and stepping that was performed at a moderate-vigorous intensity of 75–80% HRpeak. Participants used their body weight for resistance training and progressed to the use of equipment subsequently (e.g. resistance bands and light free weights). Attendance was recorded at each training session and participants wore a heart rate monitor (Polar A300, Kempele, Finland) to monitor the exercise intensity. Participants were instructed to maintain their usual dietary intake throughout the 12-week intervention period, which was assessed monthly to ensure the maintenance of usual dietary intake as previously described62. Before and following the 12-week intervention, body composition, cardio-respiratory fitness were determined and adipose tissue samples were collected from abdominal and gluteal SAT for the subsequent analysis of gene expression as detailed below.
To dissect the contribution of cell type to whole adipose tissue gene expression we included an independent cohort of 21 women and 8 men of European Ancestry, with a mean age of 42.2 ± 12.9 years and mean BMI 49.9 ± 8.4 kg/m2. From this cohort, adipocytes and cells from the stromal vascular fraction (SVF) were isolated from paired abdominal SAT samples as described previously63. Abdominal SAT was collected during open or laparoscopic abdominal surgery64 and immediately frozen in liquid nitrogen and stored at −80 °C. The study was approved by the Ethics Committee of the University of Leipzig (approval no: 159‐12‐21052012) and performed in accordance with the Declaration of Helsinki. All participants gave written informed consent before taking part in this study.
Basic anthropometry (weight, height, waist and hip circumference) was measured. Whole-body composition (including fat mass and fat-free soft tissue mass) and regional body fat distribution (gynoid and android fat mass) were determined by dual-energy X-ray absorptiometry (DXA; Discovery-W, software version 18.104.22.168; Hologic, Bedford, MA). VAT and SAT volumes were measured by magnetic resonance imaging (MRI; 3-Tesla Skyra Whole-body Human), as previously described65.
Cardiorespiratory fitness was determined by measuring peak oxygen consumption (VO2peak) using a walking treadmill-based (C, Quasar LE500CE, HP Cosmos, Nussdorf-Traunstein, Germany) graded exercise test that increased in gradient by 2% every minute until 16%, followed by an alternate increase in speed (0.5 km/hour) and gradient (1%) until volitional exhaustion, as previously reported61. Pulmonary gas exchange was measured by determining O2 and CO2 concentrations and ventilation to calculate VO2 consumption and respiratory exchange ratio (RER) using a metabolic gas analysis system (CPET, Cosmed, Rome Italy)61.
Adipose tissue biopsies
Before and after exercise training, whole-tissue samples from the aSAT and gSAT depots were collected by mini-liposuction after 4–6 h of fasting, and at least 48–72 h after the last exercise training session. These samples were subsequently used for RNA extraction and evaluation of the gene expression using a microarray technique.
RNA extraction, reverse transcription and mRNA expression
Total RNA was extracted from whole-tissue SAT, adipocytes and SVF using RNeasy Lipid tissue Mini kit according to the manufacturer’s protocol (Qiagen Ltd, Germantown, MD, USA). To improve the quality and yield of the microarray probes, RNA was re-purified using RNeasy mini columns (Qiagen, Hilden, Germany). The integrity and concentration of the purified RNA was verified on an Agilent Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA) using the RNA Kit (Agilent Technologies) according to the manufacturer’s instructions.
For the mRNA expression analysis of selected DEGs, 1 µg of total RNA from isolated adipocytes and SVF was reverse-transcribed using High-Capacity cDNA Reverse Transcription Kit with RNase inhibitors (Thermofisher, Darmstadt, Germany). TaqMan probe‐based quantitative real‐time polymerase chain reaction (RT-PCR) was performed on cDNA samples using the QuantStudio 6 Flex Real‐Time PCR System (Thermofisher, Darmstadt, Germany). A standard curve was constructed for each primer-probe set using a serial dilution of cDNA pooled from all samples. The expression of HOXA5 (Hs00430330_m1), DMRT2 (Hs00246364_m1), DMRT3 (Hs00253642_m1), RSPO3 (Hs00262176_m1), CS1N1 (Hs00157136_m1) and MMP9 (Hs00957562_m1) were normalized to ribosomal protein lateral stalk subunit PO (RPLPo; Hs99999902_m1) as endogenous gene and presented as the ratio of abundance of the gene of interest: abundance of RPLPo.
Array data extraction and analysis
Purified RNA samples from aSAT (Pre: n = 12; Post: n = 12) and gSAT (Pre: n = 12; Post: n = 12) were analyzed before and after exercise training using Illumina Expression BeadChip (Epicentre Biotechnologies, Madison, WI, USA). One array per tissue sample, for each SAT depot and for each time point (i.e. 4 arrays per participant × 12 participants) was used for the gene expression analysis (total of 24 arrays for gSAT samples and 24 arrays for aSAT samples). Each total RNA aliquot of 250 ng was ethanol precipitated with GlycoBlue (Invitrogen) as a carrier and dissolved at a concentration of 100–150 ng/µl before probe synthesis using the TargetAmp-Nano Labeling Kit for Illumina Expression BeadChip. 750 ng of cRNA were hybridized to Human HT-12 v4 Expression BeadChips (Illumina, San Diego, CA, USA) and scanned on the Illumina iScan instrument according to the manufacturer’s specifications. Raw expression intensities of 47,323 probes were extracted using Illumina GenomeStudio (Version 1.9.0) and the normalization, background correction and analysis were executed using R statistic software (version 3.6.1).
For the participants’ characteristics, data were presented as mean ± standard deviation. After the test of normality (Shapiro-Wilks test) and equality of variances (Barttlets’ test), the changes in cardiorespiratory fitness, body composition and dietary intake in response to exercise training were analyzed using a paired t-test. Significance levels were set at p < 0.05 and the analyses were performed using Stata Software version 13.1 (StataCorp, College Station, Texas 77845 USA).
mRNA expression analysis
The data were expressed as mean ± standard error of the mean. Wilcoxon’s test was performed to determine differences in the mRNA expression levels between adipocytes and SVF using Prism Version 6.05 (GraphPad Software, Inc., La Jolla, CA). The significant level was set to a p-value < 0.05.
Data quality control and differential gene expression analysis
The gene expression profile of aSAT and gSAT samples were processed from the raw data obtained from Illumina GenomeStudio following the protocol of Ritchie at al.66. After background correction, normalization, and log2 transformation (with neqc function), the probes annotated as “No match” or “Bad” were removed from the analysis. However, before removing, the 53 genes annotated as ‘Bad” but with high average expression intensities (>12) were further investigated. Forty-five were repetitive Alu-elements, and the remaining were ribosomal proteins, EEF1A1 (eukaryotic translation elongation factor), and FTH1 (Ferritin heavy chain) all of which were encoded at multiple genomic loci, making further analysis questionable.
The filtered expression data were subsequently used for differential gene expression analysis across the two depots, separately for each time point. We fitted a linear model and computed the moderated t-statistics, moderated F-statistics, and log-odds for the pairwise contrasts by empirical Bayes from the limma package67. This comprised correction for multiple testing (false discovery rate; FDR). Differential expression was investigated between aSAT and gSAT at baseline and post-training, as well as within each fat depot before and after exercise training. Genes were identified as differentially expressed when the difference in the expression intensities were equal or higher than 33% (i.e. fold change (FC) ≤ 0.67 for lower/downregulated genes and FC ≥ 1.5 for higher/upregulated genes) or an absolute log2 fold change (|LFC | ) ≥ 0.58, with a p-value ≤ 0.05.
Gene Set Enrichment and In Silico Promotor Analyses
Enriched biological processes and functional pathways represented by the identified DEGs were obtained from STRING68. Subsequently, the number of expected genes in a random set of the same size were compared to the number of observed DEGs assigned to a specific enriched GO-term. The DEGs were subsequently analyzed for over-represented motifs in their promoter regions using HOMER (hypergeometric optimization of motif enrichment) v4.1069. Detailed method and data processing of each comparison set (aSAT vs gSAT in both time point, aSAT pre- vs post-exercise training and gSAT pre- vs post-exercise training are provided in the supplementary document.
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We are thankful to all women that participated in this study, to the field workers who assisted with the recruitment of participants; and the laboratory technicians of the Department of Endocrinology and of the Core Unit DNA-Technologies of the University of Leipzig who assisted with the sample analysis. This study was supported by the National Research Foundation of South Africa (NRF), Competitive Programme for Rated Researchers (Grant no: 93577) and by the Deutsche Forschungsgemeinschaft (SFB1052/02, # 209933838, B01).
The authors declare no competing interests.
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Nono Nankam, P.A., Blüher, M., Kehr, S. et al. Distinct abdominal and gluteal adipose tissue transcriptome signatures are altered by exercise training in African women with obesity. Sci Rep 10, 10240 (2020). https://doi.org/10.1038/s41598-020-66868-z