## Introduction

Human exposure to aluminium is burgeoning and its entry into and presence within the human body is inevitable1,2,3. There is no ‘aluminium homeostasis’4. The bioinorganic chemistry of aluminium dictates that it will ‘piggy-back’ upon essential biochemistry and it is such adventitious interactions that determine its fate in the human body. The brain is a target tissue for accumulation of aluminium5,6. Long-lived neurones provide intracellular pools, such as citrate, ATP and glutamic acid, where aluminium can remain complexed without necessarily disrupting cellular biochemistry7. Aluminium is neurotoxic and is found in brain tissue in extracellular milieu associated with neuropathology including senile plaques and neurofibrillary tangles in Alzheimer’s disease8,9. While there is no longer any debate as to the presence of aluminium in human brain tissue, there remains the question of how much aluminium in brain tissue is too much10. A number of recent studies have provided data on aluminium content in brain tissue in Alzheimer’s disease11, multiple sclerosis12 and autism13. The quantitative data, supported by aluminium-specific imaging, are invariably reported as being high, higher than expected. However, data on the latter, true control data are extremely rare. Brain banks have themselves struggled with the concept of what constitutes a true control14. We asked one such brain bank to identify a set of donor brain tissues that could act as a control for brains affected and diagnosed with a neurodegenerative disease. The majority of control brains available through brain banks are from older donors and so most still show some signs of age-related degeneration. Herein we have measured the aluminium content of twenty control brains where in each case there was no overt neurodegeneration, no diagnosis of a neurodegenerative disease but some age-related changes in the older donors. We have then compared these data with data, measured under identical conditions, for donors having died with diagnoses of Alzheimer’s disease, multiple sclerosis and autism.

## Results

### Control brain tissues

The aluminium content of all tissues ranged from 0.01 (the limit of quantitation) to 9.28 μg/g dry wt. (Table 1). The majority of tissues (150 out of 191) were below 1.00 μg/g dry wt. though 28, 6 and 7 tissues were in the range 1.00–1.99, 2.00–2.99 and ≥3.00 μg/g dry wt. respectively. The aluminium content of each lobe (mean and SD) were 1.03 (1.64), 1.02 (1.27), 0.95 (0.88), 0.77 (0.92) and 0.51 (0.51) μg/g dry wt. for frontal, temporal, parietal, occipital and cerebellum respectively. There were no statistically significant differences between aluminium content and age (p-value = 0.7656) or gender (p-value = 0.4005) and even though the cerebellum had the lowest content of aluminium, there were no statistically significant differences between any of the five brain regions (p-value = 0.2488; Table 2; Fig. 1).

### Comparison with disease groups

Treatment group (sAD; sAD–; sAD + ; fAD; ASD; MS) was the only factor that consistently affected the aluminium content of donor brain tissue. This was the case for all statistical analyses carried out including; when units were unweighted observations or means of individuals; for both adjustments sAD+ and sAD–; for truncation of outliers; parametric or non-parametric procedures (Table 4). The aluminium content of brain tissue in the control group was significantly lower than sAD (P = 0.0006), fAD (P = 0.0020), ASD (P = 0.0123) and MS (P < 0.0001) (Fig. 2).

## Discussion

We present the first comprehensive data set for the aluminium content of brain tissue in donors without a diagnosis of neurodegenerative disease. All donors fulfilled recently revised criteria for control brain tissues14. Approximately 80% of measured tissues have an aluminium content below 1.0 μg/g dry wt. (Table 1). There are some anomalies, 6 out of 191 tissues have an aluminium content ≥3.00 μg/g dry wt., and these are worth future investigation to identify possible neuropathology. There was no statistically significant relationship between brain aluminium content and age of donor and this observation is contrary to a previous investigation of brain aluminium in a neurologically normal population15. An explanation may be that herein only two out of twenty donors were below 66 years old. The data do support a conclusion that a high content of brain aluminium is not an inevitability of ageing.

Aluminium is not a member of the human metallome4. However, its omnipresence in human tissue and especially the brain cannot be without consequence. It is only inimical to life, there is no homeostasis, and it is always a burden to life’s processes. Every atom of aluminium in human brain tissue must be accommodated as aluminium as Al3+(aq), is highly biologically reactive. Life is robust and some aluminium in human brain tissue is tolerated without overt effects. We need to define such limits in the terms of both quantity and location and we need to be more fully aware of human exposure to aluminium. We may then live healthily in the aluminium age (https://www.hippocraticpost.com/mens-health/the-aluminium-age/).

## Methods

### Tissues

Brain tissues were obtained from the London Neurodegenerative Diseases Brain Bank following ethical approval (NRES Approval No. 08/MRE09/38+5). Donor brains were chosen on our behalf by the consultant neuropathologist at the brain bank. All had a clinical diagnosis of ‘control’ while some had a pathological diagnosis that included age-related changes in tissue. There were five male and fifteen female donors. They were aged between 47 and 105 years old. Tissues were obtained from frontal, occipital, parietal and temporal lobes and cerebellum from all donors.

### Quantitative measurements

The aluminium content of tissues was measured by an established and fully validated method16 that herein is described only briefly. Samples of cortex, approximately 1 g in weight, were thawed at room temperature and cut using a stainless steel blade into sections approximately 0.5 g in weight. Tissues were dried for 48 h to a constant weight in an incubator at 37 °C. Dry and thereafter weighed tissues were digested in a microwave (MARS Xpress CEM Microwave Technology Ltd.) in a mixture of 1 mL 15.8 M HNO3 (Fisher Analytical Grade) and 1 mL 30% w/v H2O2 (BDH Aristar). Resulting digests were clear with no fatty residues and, upon cooling, were made up to 5 mL volume using ultrapure water (cond. <0.067μS/cm). Total aluminium was measured in each sample by transversely heated graphite furnace atomic absorption spectrometry (TH GFAAS) using matrix-matched standards and an established analytical programme alongside previously validated quality assurance data. The latter included method blanks, detailed descriptions of which have been published recently10,16.

### Statistical analyses

#### Comparisons between brain lobes were analysed using control group data

The control group dataset was well balanced with repeated measurements. Analyses were performed using the SAS general linear models (GLM) procedure, including gender, age and lobes as factors. Observations (OB) was the unit of analysis. The programme and raw data are available upon request. We considered a p-value smaller than 0.05 to be statistically significant using the model shown below.

$$Model:\,OB=gender+age+lobe+gender\ast age+gender\ast lobe+lobe\ast age+error$$

#### Analysis by disease group

The distribution of aluminium content data is heavily skewed in the treatment groups. Data are not balanced with the number of observations and their respective lobe varying considerably between treatment groups. There is large variability in repeated measurements taken from the same donor. Analyses were performed for both the unweighted observations, and means across all lobes for each individual.

The assumption that the data across all groups are normally distributed, an assumption that underlies any ANOVA model, is questionable at best. A non-parametric approach was used to avoid this assumption. For the non-parametric tests, the SAS NPAR1WAY procedure was used, with the Dwass, Steel, Critchlow-Fligner Method for two-way comparisons between the different disease categories.

Null and Alternative hypothesis

H0:: µi = µj for all ij (No differences between means of control and disease groups)

H1: µiµj for some ij (At least one difference between means of control and disease groups)