Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

# Effect of TAT-DOX-PEG irradiated gold nanoparticles conjugates on human osteosarcoma cells

## Abstract

The paper aims to investigate the cytotoxic effect on tumor cells of irradiated AuNPs in green light and subsequently functionalized with HS-PEG-NH2. The toxicity level of gold conjugates after their functionalization with DOX and TAT peptide was also evaluated. The AuNPs were prepared using the modified Turkevich method and exposed to visible light at a wavelength of 520 nm prior their PEGylation. The optical properties were analyzed by UV-vis spectroscopy, the surface modification was investigated using FTIR and XPS spectroscopies and their sizes and morphologies were evaluated by TEM and DLS techniques. DOX and TAT peptide were linked to the surface of PEGylated AuNPs by reacting their amino groups with glycidyloxypropyl of PEGylated DOX or TAT conjugates under mild conditions at room temperature and in the presence of ethanol as catalyst. The conjugates containing DOX or DOX and TAT have been characterized by fluorescence and FTIR techniques. The changes of electrochemical features were observed using cyclic voltammetry, suggesting a better stability of irradiated nanoparticles. By mass spectrometry it was confirmed that the compounds of interest were obtained. The cell viability test showed that irradiated and non-irradiated nanoparticles coated with PEG are not toxic in normal cells. Tumor cell viability analysis showed that the PEGylated nanoparticles modified with DOX and TAT peptide were more effective than pristine DOX, indicating cytotoxicity up to 10% higher than non-irradiated ones.

## Introduction

The presence of gold nanomaterials (AuNPs) in biomedicine and particularly in antitumor therapy still remains a topic of wide debate, as evidenced by the tremendous amount of scientific works on this issue in recent years1,2,3. An impressive number of research studies have straightened their efforts toward the use of AuNPs in enhancing the efficiency of cancer treatment, due to their ease production and chemical functionalization of their surface4,5. Gold nanoparticles are feasible to be developed as versatile nontoxic carriers for drug release as long as they are able to be conjugated with different molecules, including chemotherapeutics, antibodies, peptides, ligands, and other structures which are likely to promote a great capacity to penetrate the tumor site, resulting in a predominant accumulation of bioactive agent in the tumor region6,7. On the other hand, the passive anticancer effect based on the accumulation strategy of AuNPs at the tumor site is limited by the inherent heterogeneities of tumor vasculature8. It was shown that nanoparticle concentration in the target tissue is influenced by renal clearance rate, and also by activation of immune system mechanisms such as opsonization or nonspecific particle phagocytosis, fulfilled by the reticuloendothelial system (RES).

Different strategies for surface functionalization of AuNPs using a wide range of ligands have been done to overcome these limitations. Polyethylene glycol (PEG) is the polymer known as the most popular material for surface modification in various types of nanoparticulate drugs or gene delivery systems9,10,11,12,13. The coverage of the conjugates with PEG moieties plays a major role in improving solubility and stability in aqueous media of the carriers, prolonging the circulation time in the blood stream, bypassing immune recognition due to steric hindrance mechanism14,15,16.

The present work aimed to conduct insightful studies of biological effects starting from previous outcomes involving polymer-coated gold nanoparticles subjected to green light irradiation. According to a previous research17 concerning fungal cultures treated with AuNPs suspensions, the first line of defense, expressed by antioxidant enzymes, was quantified by analyzing the activity of superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) content. The greatest stimulation of CAT and SOD was induced after incubation of cellulolytic fungi cultures with gold nanoparticles irradiated with green light, as compared to similar nanoparticles irradiated with other wavelengths. Since an increased level of ROS has been generated after incubation of fungal cells in the presence of green light irradiated AuNPs, similar irradiated particles are expected to produce comparable effects on human cells, inhibiting the growth of tumor cells.

The nanoparticles were designed to be irradiated just after their stabilization in sodium citrate, for the reason that at this stage the metal atoms could modify their oxidation state with an influence on organic layer conformation18. A significant number of works have been concerned about the structure of citrate adlayers on gold nanoparticles, elucidating the binding modes of carboxylate to metallic surface as a consequence of reaction parameters18,19,20,21. Starting from the classical Turkevich method, the synthesis was enhanced by adding sodium hydroxide to the reaction medium (see Material and Method section), thus obtaining a stable colloidal solution having a concentration ten times higher in gold than in a traditional protocol (from 0.25 to 2.5 mM)22. Further polymer coating with PEG, as it can be seen later in this paper, is logically influenced by the oxidation states of gold atoms and also the binding feature between citrate and particle surface23,24. The PEGylation using a heterobifunctional polyether derivative was designed to displace the citrate ligand due to formation of stronger Au-S coordinative bonds, although Au-COO from citrate linkages may also be present, explaining the sporadic occurrence of amine terminal end of PEG in gold surface vicinity, based on electrostatic interaction between -COO and -NH3+ 25,26,27.

A different approach of antitumor therapy was considered in the framework of current research, by which doxorubicin (DOX), one of the most investigated chemotherapeutic agents, was covalently bound to the surface of polymer coated gold nanoparticles28. The coupling was envisioned through an oxirane bifunctional linker, able to bind both DOX and PEG due to the presence of a primary amino group29. A second important issue was to achieve an efficient penetrability into the tumor cells by the drug loaded particles. Thus, a small number of TAT-peptide grafted onto the polymeric shell has been found as a convenient strategy, with acknowledged results in cellular internalization of non-self-structures and with a highly potential of tumor targeting, as reported in literature30,31,32. FTIR and XPS spectroscopy proved to be of crucial importance in establishing the structure of the final products, with a focus on the differences produced by irradiation. The structure elucidation was completed by morphological and dimensional data performed by TEM and DLS, which reveal uniform entities not exceeding 20 nm in diameter, as disclosed later in this work. The positive ζ potential of the nanoparticles, due to the protonated amino groups onto the polymer coating surface, provide the advantage of being opposite to the cell surface charging, thus facilitating their transfer through cell membranes33.

Biological tests have revealed that our drug-free carriers (AuPEG2000-NH2 and iAuPEG2000-NH2, comprising only the metallic core coated with PEG) do not exhibit cytotoxic effects on normal human dermal fibroblasts and human osteosarcoma. Moreover, the loaded carriers with doxorubicin were more efficient when TAT peptide was attached to the system.

## Results and discussions

### Synthesis

Following the synthesis protocol described in Materials and Methods section and illustrated by Fig. 1, a 2.5 mM suspension of AuNPs was obtained, comprising particles of approximately 17 nm in diameter (16.83 ± 0.25 nm) with long-term stability (over 6 months, determined by macroscopic evaluation and confirmed by DLS and UV-Vis spectroscopy). The enhanced stability of the AuNPs suspension is mainly due to the use of a high pH value during AuNPs formation process in the presence of sodium citrate22. It is well known that at high pH, the citrate is fully deprotonated, creating a high abundance of negative charges, inducing repulsions between nearby gold nanoparticles and as a result no aggregation can be obtained.

The DOX-PEG500-epoxy and TAT-PEG500-epoxy precursors were synthesized according to the protocol described in Materials and Methods section (see Supplementary scheme S2 and S3), and characterized by ESI-MS (see Fig. S4 and S5 and section III from Supplementary Information).

### Nanoparticles characterization

By green light irradiation of the nanoparticles, several modifications could be observed regarding the surface properties, compared with non-irradiate ones. These changes were explored both morphologically and structurally, using imaging and spectroscopic techniques, respectively. Consequently, some differences in the oxidation states of the chemical species involved were detected, influencing the chemical bonds established in the nanoparticulate compound. Starting from these findings, our studies further pursued the effect of irradiation of AuNPs on subsequent functionalization as well as their biological activity.

UV-Vis spectroscopy data, well-known to assess the gold nanoparticle characteristics, principally related to size and concentration, in close connection with imaging data34,35, have been detailed and discussed (see Supplementary data, section II.1). TEM imaging data bring essential information regarding the dimension and particle organization. A significant number of images have been acquired and analyzed of which the most representatives are presented below in this paper (Fig. 2); the Fig. S6 in supplementary material comprises overall TEM images of the same compounds, which show more clearly the organization of nanoparticles relative to each other (e.g. clustering, if applicable). TEM micrographs of AuNPs have shown to be predominantly spherical with irregular shapes and displaying a satisfactory dimensional distribution (Figs. 2a and S6a), with a mean diameter of 17 nm. In contrast, iAuNPs shows more regular profiles, having a mean diameter of 16 nm, with the tendency to form clusters of undefined shapes (Figs. 2b and S6b). TEM images of PEGylated nanoparticles indicate an increase in particle size (from 16–17 to 22 nm) alongside with a higher level of clustering36. As revealed by the Fig. 2d, the irradiated particles (iAuPEG2000-NH2), illustrates an agglomeration tendency, as compared to the non-irradiated ones (Fig. 2c), but in the first case the entities are arranged in groups with regular forms of less than 80 nm in diameter. The formation of these small associations was also confirmed by DLS investigations, where the measured values for the hydrodynamic diameter disclose the presence of two populations: one with tens of nm values representing the free particles, and another around 400 nm representing clustered particles (see Supplementary Table S3). The agglomeration trend in the AuPEG2000-NH2/iAuPEG2000-NH2 compounds can be explained either by the occurrence of the S-S cross-linking between the polymer chains at 22 °C, but also due to an increase in ζ potential from –40 mV in AuNPs to about +28 mV after PEGylation37.

FTIR spectroscopy, in close correlation with XPS data, highlighted the structure differences between the irradiated compounds versus non-irradiated ones, which originate in the type of interaction between citrate ions and gold atoms, more precisely concerning the conformation adopted by the citrate molecules involved in gold nanoparticle stabilization. The spectra of all intermediate products (AuNPs, iAuNPs, AuPEG2000-NH2 and iAuPEG2000-NH2) are presented and extensive discussed in section IV, Supplementary Information available.

Furthermore, XPS analysis was also carried out to investigate the surface oxidation states and chemical composition of the irradiated compounds versus non-irradiated ones. The C1s signal in the AuNPs suggests that COO groups bind to gold by two coordination modes: η2 Au-COO (chelating) at 286.7 eV and η1 Au-COO (bridging) at 288.1 eV. In iAuNPs the COO group binds to the gold surface by a single η1 Au-COO (chelating) coordination mode at 288.2 eV. The high-resolution spectra C1s (Fig. 3A) revealed the presence of C-H/C-C, C-O, C-N, C-S, COO and COOH bonds at 284.6, 286.2, 288.2, 289, 284.4 and 289.5 eV for both AuPEG2000-NH2 and iAuPEG2000-NH2 compounds23. The lower percentage of C-O bond, correlated to the smaller percent of COO and COOH, in iAuPEG2000-NH2 than in AuPEG2000-NH2, suggests that the amount of remaining citrate is significantly reduced in the case of irradiated nanoparticles. Additionally, in the iAuPEG2000-NH2 conjugates, the C1s spectrum confirmed the presence of the bridging mode η1 Au-COO and the disappearance of the chelation mode (Fig. 3A).

To obtain AuPEG2000-DOX, iAuPEG2000-DOX, AuPEG2000-TAT-DOX and iAuPEG2000-TAT-DOX conjugates, the amino terminal groups of AuPEG2000-NH2 or iAuPEG2000-NH2 were reacted with epoxy groups of DOX-PEG500-epoxy and/or TAT-PEG500-epoxy (see Supplementary Scheme S1 and S2) water solutions in the presence of isopropanol (as catalyst) in a molar ratio OH: epoxy groups added in the system was 1: 1, according to literature data38. The grafting reactions were carried out taking into account the molar ratio between the participating molecular species HS-PEG2000-NH2: DOX-PEG500-epoxy: TAT-PEG500-epoxy of 10: 1: 0.1 molar ratio which is equivalent to the ratio Au: DOX-PEG500-epoxy: TAT-PEG500-epoxy of 1: 2.3: 0.23. It should be noted that the HS-PEG2000-NH2 is linked to the AuNPs surfaces and it was considered that the ratio of 1: 20 between gold and HS-PEG2000-NH2 remained unchanged after purification of AuPEG2000-NH2 or iAuPEG2000-NH2 conjugates. The mixtures were maintained under stirring at 18 °C for 72 hours. As it was mentioned in Materials and Methods section, DOX-PEG500-epoxy conjugates were used without purification in subsequent reactions with AuPEG2000-NH2 or iAuPEG2000-NH2 nanoparticles, meaning that the small amount of free doxorubicin added together with DOX-PEG500-epoxy conjugates can involve many hydrogen bonds with donor/acceptor moieties due to the presence of multiple hydroxyl and carbonyl groups in its composition, making possible its stabilization onto polymer chains surrounding the newly formed nanoconjugates, expressed by the codes: AuPEG2000-DOX, iAuPEG2000-DOX, AuPEG2000-TAT-DOX and iAuPEG2000-TAT-DOX. This finding was demonstrated before by Hutchins39 when DOX formed hydrogen bonds with PEG moiety from PE-b-PEG copolymers. Under this circumstance, the “free” DOX is assumed to leave the carrier in the first step, prior to release of the drug in major amount, which was covalently bound onto the polymer layer of the gold nanoparticles.

The redox behavior of non-irradiated gold nanoparticles (AuNPs, AuPEG2000-NH2 and AuPEG2000-DOX) deposited onto electrodes was compared with that of irradiated analogues (iAuNPs, iAuPEG2000-NH2 and iAuPEG2000-DOX) (Fig. 4). The cyclic voltammetry of non-irradiated AuNPs (Fig. 4a) shows two anodic peaks located at +0.77 V ascribed to the oxidation of metallic gold to gold oxide (Eq. 1) and the second peak at +1.07 V due to the oxidation of gold to the trivalent state (Eq. 2)40,41.

$$2{{\rm{Au}}}^{0}+{{\rm{H}}}_{2}{\rm{O}}\to {{\rm{Au}}}_{2}{\rm{O}}+2{{\rm{H}}}^{+}+2{{\rm{e}}}^{-}$$
(1)
$${{\rm{Au}}}^{0}+3{{\rm{H}}}_{2}{\rm{O}}\to {\rm{Au}}{({\rm{OH}})}_{3}+3{{\rm{H}}}^{+}+3{{\rm{e}}}^{-}$$
(2)

Anodic processes are accompanied by one cathodic peak around +0.47 V in the reverse scan, associated to the reduction of gold oxide species back to metallic gold, especially with the reduction of Au3+42. The same oxidation peaks are also observed for iAuNPs (Fig. 4b), with an enhanced oxidation current for the second peak located at +0.77 V and less defined oxidation peak at +1.07 V, which can be related to the strong effect of irradiation which creates more sites prone to first electronic transfer. The oxide film which is formed in the positive scan is not entirely reduced in the reverse scan as seen by the smaller reduction peak. This indicates that higher energy is required for its reduction compared to non-irradiated nanoparticles. By analyzing cyclic voltammetry, it is highlighted that for non-irradiated gold nanoparticles there is an increase in oxygen binding to two gold atoms followed by their reduction, while for irradiated nanoparticles this reduction of oxide film is almost vanished, suggesting a better stability of irradiated nanoparticles. The electrochemical properties of AuNPs are considerably diminished by PEG coating and further by coverage with DOX. It has also been observed a slight increase of current for the first oxidation peak when particles are covered by DOX, which is attributed to the redox activity of DOX (which shows oxidation at the same potential, data not shown). The lack of reduction peaks for AuPEG2000-DOX and iAuPEG2000-DOX might be attributed to the passivation of Au nanoparticles with a monolayer of DOX.

Figure 5 presents the fluorescence emission spectra of pristine DOX and of conjugates at excitation wavelength λex of 480 nm. The fluorescence emission spectra of pristine DOX as a function of its concentration present two characteristic emission peaks at 556 and 590 nm with decreasing in their intensities once the concentration of DOX decreases (Fig. 5A). From the same figure it can be observed that the fluorescence intensities of all studied conjugates have decreased compared to those of pristine DOX at the same DOX concentration in the series: DOX > iAuPEG2000-DOX > AuPEG2000-DOX > DOX-PEG500-epoxy> AuPEG2000-TAT-DOX > iAuPEG2000-TAT-DOX, leading to the idea that with the increase of PEG concentration in the system the quenching effect on DOX photoluminescence is more pronounced. In this idea, the emission spectra vs. the concentration of DOX-PEG500-epoxy intermediate were registered (Fig. 5B). From this figure and from the variation of the peak intensities at λem = 590 nm (Fig. 5C) or λem = 556 nm (Fig. 5D) as a function of PEG concentration, the intensities of the peaks increased up to a certain concentration and then began to decrease with further increase in PEG concentration along with that of DOX. In this context, we can conclude that the presence of PEG in the DOX-PEG500-epoxy solutions manifests a quenching effect on DOX, depending on PEG concentration, which indicate on the one hand that DOX was bounded to PEG molecule43 and on the other hand, being also suitable to involve hydrogen bonds with PEG moieties44. The emission intensities of DOX-PEG500-epoxy, AuPEG2000-DOX and iAuPEG2000-DOX solutions at higher DOX concentration (around 0.2 mg/mL) are in the same range (Fig. 5A), deducing the fact that the PEG moieties from DOX-PEG500-epoxy precursor has a major influence in the decreasing of DOX photoluminescence. This remark was not sustained when we decreased the concentration of DOX together with that of Au conjugates from the studied solutions (Fig. 5C,D), in fact irradiated or non-irradiated Au-PEG conjugates together with PEG from DOX-PEG conjugates contributed to the quenching effect of DOX photoluminescence. Also, it should be added that fluorescence quenching effect on DOX is more pronounced after the addition of TAT to either irradiated or non-irradiated conjugates (Fig. 5A,C,D).

The fluorescence emission spectra of AuPEG2000-DOX, iAuPEG2000-DOX, AuPEG2000-TAT-DOX, iAuPEG2000-TAT-DOX conjugates do not give information about the composition of the conjugates, giving information about the behavior of DOX (as fluorescence probe) in different microenvironments45. Because of this reason FTIR spectra are necessary to confirm the presence of ligands on AuNPs layers. Figure 6 presents the FTIR spectra of AuPEG2000-DOX, iAuPEG2000-DOX, AuPEG2000-TAT-DOX, iAuPEG2000-TAT-DOX conjugates along with that of DOX-PEG500-epoxy, AuPEG2000-NH2 and iAuPEG2000-NH2 intermediates. The spectra were normalized taking into consideration the band of 1390 cm−1 (C-H bending vibration) which does exist in all represented spectra. The spectra shown four major characteristic regions: the first (400–750 cm−1) is related to AuNPs bonding with sulfur (Au-S), remaining oxygen from citrate, and C-S from HS-PEG2000-NH2; the second one (750–1300 cm−1) is attributed to C-O stretching vibrations; the third region (1300–1800 cm−1) is represented by C=O stretching, amide I (stretch), amide II and III, C=C stretch and C-H bending and the last one (2800–3600 cm−1) is characteristic for O-H, N-H and C-H stretching vibrations. The FTIR spectra of AuPEG2000-DOX and iAuPEG2000-DOX present DOX characteristic bands in 1100–1800 cm−1 interval, and are sifted to those in DOX-PEG500-epoxy and AuPEG2000-NH2, being a sign that there are interactions between AuPEG2000-NH2 and DOX-PEG500-epoxy46. The region of 3400 cm−1 was not discussed because there are overlapping of specific characteristic bands of O-H and NH stretching vibrations. The bands at 2924 and 2860 cm−1, specific for C-H stretching vibrations, are more intense and better resolved.

In the FTIR spectra of AuPEG2000-TAT-DOX, iAuPEG2000-TAT-DOX the bands at 1676 (amide I), 1583 cm−1 (amide II) and 1392 (amide III) or 1668, 1587 and 1392 cm−1 respectively are well resolved with their intensity higher than 1, meaning the capping of peptide molecules on the surface. All assignments of bands are presented in Table S4 from Supplementary Information available.

### Biological assay

The final compounds AuPEG2000-DOX, iAuPEG2000DOX, AuPEG2000-TAT-DOX and iAuPEG2000-TAT-DOX were subjected to biological testing as they resulted from the last synthesis step, without further purifications. This strategy relies on the fact that unreacted precursors DOX, cys-TAT, DOX-PEG500-epoxy and TAT-PEG500-epoxy may be physically adsorbed on gold surface due to the presence of NH2 groups, or among PEG chains that are able to engage in hydrogen bonding44.

To evaluate the toxicity of the drug-free carriers (AuPEG2000-NH2 and iAuPEG2000-NH2), we have used a metabolic assay (MTS) which measures the mitochondrial reductase activity in HOS or NHDF cells incubated with the irradiated and non-irradiated carrier at specific concentrations (0.5, 0.1, 0.05 and 0.01 μg/mL) as revealed by Fig. 7. Initially, volumes of 1000 µL stock solution of 1 mg/mL were prepared starting from both AuPEG2000-NH2 and iAuPEG2000-NH2 suspensions as obtained after the removal by centrifugation of the HS-PEG2000-NH2 excess, having the same concentration of 8.04 μg/mL, calculated by taking into account the concentration of Au and HS-PEG2000-NH2, 0.2·10−3 mM and 4·10−3 mM, respectively, which corresponds to 0.0394 and 8 mg existing in 1000 µL (as calculated and presented in Supplementary Information file). Successive dilutions have been made afterwards, in order to reach the above-mentioned concentrations, which were further subjected to cytotoxicity assay.

Cells with healthy mitochondrial function could reduce tetrazolium salt to formazan and as a result, the mitochondrial reductase activity is used as an indirect indicator for cell viability. As shown in Fig. 7b, the treatment of all compounds in concentrations of up to 0.5 μg/mL did not reduce the viability of NHDF cells after 48 h of treatment, whereas the same concentrations of compounds caused a slight reduction in the HOS cell survival as can be seen in Fig. 7a. Analyzing comparatively the results obtained on the two cell types, we can infer that carriers per se are non-cytotoxic, with a pronounced proliferation effect when normal cells are involved47.

Irradiated and non-irradiated carriers loaded with doxorubicin were tested on HOS cells in two forms: enhanced with TAT peptide and without peptide (Fig. 7c). Naked doxorubicin was used as control, in the same concentrations as drug loaded nanoparticles: 10, 1, 0.1, 0.05 and 0.01 µg/mL.

The samples comprising doxorubicin were prepared at 100, 10, 1, 0.1, 0.05 and 0.01 µg/mL as presented in Section VI from Supplementary Information, available.

Analyzing the results obtained by MTS assay, synthesized by Fig. 8c, one can observe that AuPEG2000-TAT-DOX and iAuPEG2000-TAT-DOX are slightly more active than AuPEG2000-DOX and iAuPEG2000-DOX.

TAT is a cell-penetrating peptide. Cell-penetrating peptides are usually short peptides which cross cellular membrane and could be used to facilitate the cellular uptake of various molecular cargoes which are otherwise hard to transfer inside the cytoplasm48. Increased activity of compounds coupled with TAT could be explained by the ability of this peptide to enhance cell penetration.

A second observation is an enhanced effect, but not significant of irradiated compounds iAuPEG2000-DOX and iAuPEG2000-TAT-DOX at 1 µg/mL and 0.01 µg/mL compared to non-irradiated forms.

Regarding the overall activity of our gold nanoparticles loaded with doxorubicin, since 10 µg/mL of AuPEG2000-TAT-DOX contains less than 0.3 µg/mL of pure doxorubicin (see Materials and Methods section), we could infer that they are more active than doxorubicin alone. More specifically, when treating cells with 10 µg/mL pure doxorubicin, the cell viability is very close to that encountered in cells treated with a suspension of 10 µg/mL drug loaded nanoparticles: 42% ± 2.1 versus 47% ± 2.6, respectively. This finding means that the conjugate iAuPEG2000-TAT-DOX exerts approximately the same effect as the pure drug, but using a 97% lower amount of DOX. The same doxorubicin conjugates tested on NHDF cells exhibited a similar behavior with a slightly lower cytotoxicity (Fig. 7d).

## Materials and Methods

### Materials

Gold(III) chloride trihydrate (HAuCl4·3H2O, Mw = 393.83 g/mol), sodium hydroxide (NaOH), sodium citrate (Na3C6H5O7·2H2O), polyethylenglycol diglycidyl ether (PEG500, Mn ~ 500 g/mol) and doxorubicin (DOX, Mw = 579.98 g/mol) were purchased from Sigma Aldrich. Thiol PEG amine hydrochloride (HS-PEG2000-NH2·HCl, Mn ~ 2000 g/mol) was obtained from JenKen Technology USA, and cysteine-terminated TAT peptide (TAT-cys, Mw = 1315 g/mol) of 95% purity (GRKKKRRQRC) from ChemPeptide Limited company.

### Cell culture

Human osteosarcoma (HOS) and Normal Human Dermal Fibroblasts (NHDF) cell lines were purchased from CLS Cell Lines Service GmbH and PromoCell, Eagle’s minimal essential medium (MEM) from Lonza, fetal bovine serum (FBS) from Biochrom GmbH, Germany and 1% Penicillin-Streptomycin-Amphotericin B mixture (10 K/10 K/25 µg in 100 ml) from Lonza, CellTiter 96® Aqueous One Solution Cell Proliferation Assay from Promega, Trypsin-Versene (EDTA) mixture from Lonza, phosphate buffered saline (PBS) from Invitrogen.

### Synthesis of PEGylated gold nanoparticles conjugated with doxorubicin

The main synthesis steps are detailed as follows, being presented by the overall reaction scheme (Fig. 1) comprising both formation and stabilization of the particles with citrate, and then PEG, as well as the particle conjugation with TAT peptide and doxorubicin by covalent coupling.

### Synthesis of citrate stabilized gold nanoparticles (AuNPs)

In a preceding stage, Stock solutions of HAuCl4 (25 mM) and NaOH (20 mM) in deionized water were prepared. The gold nanoparticles (AuNPs) with a reproducible size of 17 nm were synthesized using the modified Turkevich method49, gaining a narrow size distribution by adding sodium hydroxide into the reaction mixture as reported by Li et al.22. Briefly, in a round flask of 100 mL, immersed in a heated water bath with magnetic stirrer, 5 mL HAuCl4 25 mM (0.125 mmol) and 16.5 mL NaOH 20 mM (0.33 mmol) were added and the final volume was adjusted up to 50 mL with deionized water to obtain a solution of 2.5 mM HAuCl4. The mixture was heated for 30 min., when the system has reached 90 °C. In the magnetic stirring conditions, 1.5 mL of sodium citrate (50 mM) was added and the heat source was maintained a further 15 min. The red wine suspension was cooled to 4 °C to prevent aggregation or growth of the AuNPs.

### Exposure of AuNPs to visible light

The nanoparticle irradiation was performed using an illumination system comprising of an ebonite tube of 24.5 cm length, equipped with a light source (a 50 W helium lamp) and a collimation system, at its upper end, and with a green photo filter positioned at the other end of the same tube (Fig. 8). 10 mL of the prepared AuNPs suspension (2.5 mM) were added in a Petri dish (having the same diameter as ebonite tube, 38 mm) in order to be irradiated. The sample was exposed to green light in four cycles of 20 min lighting between 5 min darkness – to keep the sample temperature around 25 °C. The illumination level of irradiated samples was done with Solar Light PMA 2100 device, equipped with PMA 2130 sensor. The primary energy emitted by the source was 20.6 W/m2, corresponding to an energy absorbed of 3.22 kJ/m2.

### Synthesis of PEGylated gold nanoparticles

#### PEGylation of the non-irradiated AuNPs (AuPEG2000-NH2)

Ten mL of 0.25 mM non-irradiated AuNPs suspension (2.5·10−3 mmol), obtained by 1:10 dilution with ultrapure water (0.05 µS·cm−1) of the 2.5 mM AuNPs suspension, were mixed with 100 mg HS-PEG2000-NH2.HCl (0.05 mmol) in order to gain a ratio between thiol ligand and gold of 20:1. The mixture was kept under vigorous magnetic stirring for 24 h. The resulted suspension was sonicated for 10 min on bath water (mild conditions) and then centrifuged for 60 min at 5000 rpm to remove the thiol ligand excess and citrate. The supernatant was collected and replaced with a similar volume of deionized water. This purification procedure was repeated two times, followed by resuspension of PEGylated nanoparticles in deionized water to reach a final volume of 10 mL. After particle purification about 80% of AuPEG2000-NH2 suspension was recovered, having a concentration of 0.2 mM in gold. This percent was established based on UV-Vis measurements. A calibration curve was plot measuring the absorbance of known concentrations of unpurified AuPEG2000-NH2 between 0.25 and 0.02 mM (see Supplementary Information) and after that, using the line equation and measuring the absorbance of resulting solution at 536 nm (0.838 a.u.), the concentration of purified product was determined.

#### PEGylation of the irradiated AuNPs (iAuPEG2000-NH2)

Ten mL of 0.25 mM irradiated iAuNPs suspension (2.5·10−3 mmol) was subjected to the same procedure as in the above paragraph, using the same ratio between HS-PEG2000-NH2.HCl and Au of 20:1. 10 mL iAuPEG2000-NH2 suspension were obtained, reaching a concentration of about 0.2 mM after purification, determined from UV-Vis data, using a similar reasoning as in non-irradiated product (see the calibration curve, Supplementary Information). At 528 nm, the measured absorbance of purified product was 1.421 a.u., corresponding to a concentration of about 0.2 mM in gold.

### PEGylation of DOX (DOX-PEG500-epoxy)

The covalent binding of DOX with AuNPs took place using PEG500, as a bifunctional linker, able to bind NH2 from DOX at one end and NH2 from PEG of AuNPs coating at the other end, via oxirane opening ring (Scheme S1, SI). A stock solution of 5 mg/mL DOX was prepared by dissolving 4.6 mg DOX in 920 μL ultrapure water and then stored at 4 °C. 2.58 mg PEG500 (5.16·10−3 mmol) were added over 600 μL DOX stock solution (3 mg, 5.17·10−3 mmol) to get a molar ratio PEG500: DOX of 1: 1. This ratio allowed the formation of DOX-PEG500-epoxy product, keeping an unreacted oxirane group. 2 μl of isopropyl alcohol were also added to catalyze the oxirane cycle opening by amino groups. The reaction mixture was maintained under continuous stirring, with the help of a thermomixer set at 500 rot/min and 22 °C, for 72 hours. Completion of the reaction was confirmed by mass spectrometry as depicted in paragraph 2.4.5.

### PEGylation of TAT (TAT-PEG500-epoxy)

A stock solution of 10 mg/mL cys-TAT was prepared by dissolving 10 mg peptide in 1000 μL ultra-pure water to be stored at at 4 °C. A volume of 263 μL cys-TAT stock solution (2.63 mg) and 2 μL isopropyl alcohol were added to 100 μL aqueous solution of 10 mg/mL PEG500 (1 mg) to get a molar ratio PEG500: cys-TAT of 1: 1. For this ratio, the TAT-PEG500-epoxy product was expected to contain one free oxiranic ring. The resulted mixture was maintained for 72 hours, under continuous stirring, using a thermomixer set at 500 rot/min and 18 °C. Completion of the reaction was confirmed by mass spectrometry as depicted in paragraph 2.4.5.

### Conjugation of AuPEG 2000 -NH 2 and iAuPEG 2000 -NH 2 with DOX-PEG 500 -epoxy and TAT-PEG 500 -epoxy

Reaction of amine groups of AuPEG2000-NH2 and iAuPEG2000-NH2 with epoxy groups of DOX-PEG500-epoxy and TAT-PEG500-epoxy precursors (see Scheme 1) was conducted in presence of isopropanol for 72 h at 18 °C Briefly, in 2 mL microcentrifuge tubes were added volumes of 1000 μL AuPEG2000-NH2 or iAuPEG2000-NH2 (comprising 0.2·10−3 mmol gold and 4·10−3 mmol PEG2000), 50 μL DOX-PEG500-epoxy (0.46·10−3 mmol) and 8.33 μL TAT-PEG500-epoxy (0.046 ·10−3 mmol) and isopropanol in a molar ration of OH: mmol added epoxy groups = 1: 138. The mixtures were maintained under stirring, with the help of a thermomixer set at 500 rot/min, for 72 hours at 18 °C. The samples were used for biological tests without purifications and were noted as AuPEG2000-TAT-DOX and iAuPEG2000-TAT-DOX, respectively.

Two additional batches of drug-loaded nanoparticles were obtained, without TAT-PEG500-epoxy grafted on the surface, using the same strategy as before but without the presence of TAT-PEG500-epoxy conjugate. The samples were noted as AuPEG2000-DOX and iAuPEG2000-DOX.

AuPEG2000-DOX, iAuPEG2000-DOX, AuPEG2000-TAT-DOX and iAuPEG2000-TAT-DOX conjugates were submitted for biological tests to reveal their actions on antitumor activity.

### Structural characterization

#### Fourier-transform infrared spectroscopy (FTIR)

FTIR spectra were obtained in transmission mode using a Bruker Vertex instrument, model 70. The samples were prepared by depositing the nanoparticle suspension on KBr pellets which were then subjected to a drying process (using a UV lamp) before recording the spectra. The spectra ranged from 4000 to 400 cm−1 with a resolution of 2 cm−1.

#### X-ray Photoelectron Spectroscopy (XPS)

XPS data were achieved on an Axis NOVA instrument (Kratos Analytical, Manchester, United Kingdom), using AlKα (1486.6 eV) as X-ray source, with 20 mA current and 15 kV voltages (300 W), under a base pressure of 10−8÷10−9 Torr in the sample compartment. The incident monochromatic X-ray beam was focused on a 0.7 mm ×0.3 mm XPS area of the sample surface. The high-resolution spectra for all the elements of interest were the average of five scans acquired using a pass energy of 20 eV and a step size of 0.1 eV. The binding energy of the C 1 s peak, normalized at 284.6 eV, has been established as reference value for all binding energies. XPS data fitting was accomplished using the ESCApe software, by applying Gaussian-Lorentzian mixed function.

#### Florescence spectroscopy

Fluorescence measurements were carried out using a FluoroMax-4 spectrophotometer (Horiba, Kyoto, Japan). The emission spectra were collected using an excitation wavelength of 480 nm.

### Particles size and morphology

#### Transmission electron microscopy (TEM)

The gold nanoparticles, at different stages of the coating process, were dimensionally and morphologically examined using a Hitachi High-Tech HT7700 microscope, in a 100 kV High Resolution Mode. Small amounts of aqueous samples (15 µL) were dropped on carbon coated grids (Ted Pella), being left at room temperature until complete evaporation of the solvent. The TEM images were further analyzed using imageJ software to assess the size distribution of the nanoparticles. The histograms indicating dimensional distribution of the nanoparticles were obtained by measurements made on about 1000 particles from several TEM images, of which only representative ones will be presented.

#### The hydrodynamic diameter and ζ-potential measurements

The size and ζ-potential of gold nanoparticles were investigated by dynamic light scattering (DLS) and electrophoretic light scattering (ELS), respectively, using the DelsaNano C Submicron Particle Size Analyzer (Beckman Coulter), equipped with dual 30 mW laser diodes emitting at 658 nm. The measurements were performed at 25 °C and neutral pH, and repeated three times for each sample. The colloidal suspensions were sonicated using a Branson 200 ultrasonic bath (Branson Ultrasonic Corp.) for 1 min.

### Electrochemical characterization

Cyclic voltammetry (CV) was used to evaluate the electrochemical behavior of gold nanoparticles (AuNPs, AuPEG2000-NH2 and AuPEG2000-NH2-DOX, irradiated compared with non-irradiated) deposited by physical adsorption (3 µL) from aqueous solution on the screen-printed electrode with a planar configuration, fabricated and procured from Biosensor Laboratory, University of Florence, Italy. The working electrode is of a disk shape of 3 mm diameter made of carbon paste, while the silver reference and carbon counter electrode were symmetrically placed around working electrode. The voltammetry assays were carried out using AUTOLAB PGSTAT302N instrument from ECO CHEMIE Utrecht, The Netherlands, and a single compartment electrochemical cell of 4 ml H2SO4 0.05 M.

### Biological assay on culture cell

Cell cultures: HOS and NHDF cells were grown in tissue culture flasks with alpha-MEM medium supplemented with 10% FBS and 1% Penicillin-Streptomycin-Amphotericin B mixture. The medium was refreshed every 3 or 4 days. Once cells have reached confluency, they were detached with 1x Trypsin-Versene, washed with PBS, centrifuged at 200 x g for 3 minutes and subcultured into new tissue culture flasks.

The cytotoxicity of the samples was evaluated using the CellTiter 96® Aqueous One Solution Cell Proliferation Assay. HOS and NHDF cells were seeded into a 96-well culture plate at a density of 1 × 104 cells per well and NHDF cells were seeded at a density of 5 × 103 cells per well, in 100 µl culture medium (alpha-MEM medium supplemented with 10% FBS and 1% Penicillin-Streptomycin-Amphotericin B mixture). After 24 hours the medium was replaced with fresh media containing various concentrations of samples (10, 1, 0.5, 0.1, 0.05, 0.01 μg/mL), while the control group received only cell culture medium. The experiment was repeated three times, and for each sample minimum 3 replicates were accomplished. After 46 hours, 20 μL of CellTiter 96® Aqueous One Solution reagent was added to each well, and the plates were incubated for another 2 hours before reading the result. The absorbance was recorded at 490 nm using a plate reader (EnSight, PerkinElmer). Cell viability was calculated and expressed as percentage relative to viability of control cells (considered 100%).

## Data availability

All data generated or analyzed during this study are included in this published article and its Supplementary Information file.

## References

1. 1.

Singh, P. et al. Gold nanoparticles in diagnostics and therapeutics for human cancer. Int. J. Mol. Sci. 19, 1979 (2018).

2. 2.

Vines, J. B., Yoon, J.-H., Ryu, N.-E., Lim, D.-J. & Park, H. Gold nanoparticles for photothermal cancer therapy. Front. Chem. 7, 167 (2019).

3. 3.

Jahangirian, H. et al. A review of small molecules and drug delivery applications using gold and iron nanoparticles. Int. J. Nanomedicine 14, 1633–1657 (2019).

4. 4.

Conde, J. et al. Revisiting 30 years of biofunctionalization and surface chemistry of inorganic nanoparticles for nanomedicine. Front. Chem. 2, 48 (2014).

5. 5.

Tiwari, P., Vig, K., Dennis, V. & Singh, S. Functionalized gold nanoparticles and their biomedical applications. Nanomaterials 1, 31–63 (2011).

6. 6.

Peng, J. & Liang, X. Progress in research on gold nanoparticles in cancer management. Medicine (Baltimore) 98, e15311 (2019).

7. 7.

Sztandera, K., Gorzkiewicz, M. & Klajnert-Maculewicz, B. Gold nanoparticles in cancer treatment. Mol. Pharm. 16, 1–23 (2019).

8. 8.

Jain, S., Hirst, D. G. & O’Sullivan, J. M. Gold nanoparticles as novel agents for cancer therapy. Br. J. Radiol. 85, 101–113 (2012).

9. 9.

Ardeleanu, R. et al. Multivalent polyrotaxane vectors as adaptive cargo complexes for gene therapy. Polym. Chem. 9, 845–859 (2018).

10. 10.

Clima, L., Peptanariu, D., Pinteala, M., Salic, A. & Barboiu, M. DyNAvectors: dynamic constitutional vectors for adaptive DNA transfection. Chem. Commun. (Camb.) 51, 17529–17531 (2015).

11. 11.

Dascalu, A. I. et al. Transfection-capable polycationic nanovectors which include PEGylated-cyclodextrin structural units: a new synthesis pathway. J. Mater. Chem. B 5, 7164–7174 (2017).

12. 12.

Lazarus, G. G. & Singh, M. In vitro cytotoxic activity and transfection efficiency of polyethyleneimine functionalized gold nanoparticles. Colloids Surf. B Biointerfaces 145, 906–911 (2016).

13. 13.

Uritu, C. M. et al. Hybrid fullerene conjugates as vectors for DNA cell-delivery. J. Mater. Chem. B 3, 2433–2446 (2015).

14. 14.

Bunker, A. Poly (ethylene glycol) in drug delivery, why does it work, and can we do better? All atom molecular dynamics simulation provides some answers. Phys. Procedia 34, 24–33 (2012).

15. 15.

Cho, W.-S. et al. Size-dependent tissue kinetics of PEG-coated gold nanoparticles. Toxicol. Appl. Pharmacol. 245, 116–123 (2010).

16. 16.

Suk, J. S., Xu, Q., Kim, N., Hanes, J. & Ensign, L. M. PEGylation as a strategy for improving nanoparticle-based drug and gene delivery. ISO 4 : Adv. Drug Deliv. Rev. 99, 28–51 (2016).

17. 17.

Andries, M., Pricop, D., Oprica, L., Creanga, D.-E. & Iacomi, F. The effect of visible light on gold nanoparticles and some bioeffects on environmental fungi. Int. J. Pharm. 505, 255–261 (2016).

18. 18.

Park, J.-W. & Shumaker-Parry, J. S. Structural study of citrate layers on gold nanoparticles: role of intermolecular interactions in stabilizing nanoparticles. J. Am. Chem. Soc. 136, 1907–1921 (2014).

19. 19.

Al-Johani, H. et al. The structure and binding mode of citrate in the stabilization of gold nanoparticles. Nat. Chem. 9, 890–895 (2017).

20. 20.

MacLeod, M. J. et al. Robust gold nanorods stabilized by bidentate N-heterocyclic-carbene–thiolate ligands. Nat. Chem. 11, 57–63 (2019).

21. 21.

Polte, J. et al. Mechanism of gold nanoparticle formation in the classical citrate synthesis method derived from coupled in situ XANES and SAXS evaluation. J. Am. Chem. Soc. 132, 1296–1301 (2010).

22. 22.

Li, C., Li, D., Wan, G., Xu, J. & Hou, W. Facile synthesis of concentrated gold nanoparticles with low size-distribution in water: temperature and pH controls. Nanoscale Res. Lett. 6, 440 (2011).

23. 23.

Martynyuk, O. et al. On the high sensitivity of the electronic states of 1 nm gold particles to pretreatments and modifiers. Molecules. 21, 432 (2016).

24. 24.

Wang, L.-S. Covalent gold. Phys. Chem. Chem. Phys. 12, 8694–8705 (2010).

25. 25.

Dinkel, R., Braunschweig, B. & Peukert, W. Fast and slow ligand exchange at the surface of colloidal gold nanoparticles. J. Phys. Chem. C 120, 1673–1682 (2016).

26. 26.

Hong, R., Fischer, N. O., Emrick, T. & Rotello, V. M. Surface PEGylation and ligand exchange chemistry of FePt nanoparticles for biological applications. Chem. Mater. 17, 4617–4621 (2005).

27. 27.

Wei, H. et al. Real-time monitoring of ligand exchange kinetics on gold nanoparticle surfaces enabled by hot spot-normalized surface-enhanced Raman scattering. Environ. Sci. Technol. 53, 575–585 (2019).

28. 28.

Rivankar, S. An overview of doxorubicin formulations in cancer therapy. J. Cancer Res. Ther. 10, 853–858 (2014).

29. 29.

Young Lee, G., Park, K., Nam, J. H., Kim, S. Y. & Byun, Y. Anti-tumor and anti-metastatic effects of gelatin-doxorubicin and PEGylated gelatin-doxorubicin nanoparticles in SCC7 bearing mice. J. Drug Target. 14, 707–716 (2006).

30. 30.

Borrelli, A., Tornesello, A., Tornesello, M. & Buonaguro, F. Cell penetrating peptides as molecular carriers for anti-cancer agents. Molecules 23, 295 (2018).

31. 31.

Regberg, J., Srimanee, A. & Langel, Ü. Applications of cell-penetrating peptides for tumor targeting and future cancer therapies. Pharmaceuticals (Basel) 5, 991–1007 (2012).

32. 32.

Wang, Z. et al. Novel gelatin–siloxane nanoparticles decorated by Tat peptide as vectors for gene therapy. Nanotechnology 19, 445103 (2008).

33. 33.

Bannunah, A. M., Vllasaliu, D., Lord, J. & Stolnik, S. Mechanisms of nanoparticle internalization and transport across an intestinal epithelial cell model: effect of size and surface charge. Mol. Pharm. 11, 4363–4373 (2014).

34. 34.

Aslam, M., Fu, L., Su, M., Vijayamohanan, K. & Dravid, V. P. Novel one-step synthesis of amine-stabilized aqueous colloidal gold nanoparticles. J. Mater. Chem. 14, 1795–1797 (2004).

35. 35.

Ji, X. et al. Size control of gold nanocrystals in citrate reduction: the third role of citrate. J. Am. Chem. Soc. 129, 13939–13948 (2007).

36. 36.

Watanabe, K., Tanaka, E., Ishii, H. & Nagao, D. The plasmonic properties of gold nanoparticle clusters formed via applying an AC electric field. Soft Matter 14, 3372–3377 (2018).

37. 37.

Mudunkotuwa, I. A., Minshid, A. A. & Grassian, V. H. ATR-FTIR spectroscopy as a tool to probe surface adsorption on nanoparticles at the liquid–solid interface in environmentally and biologically relevant media. Analyst 139, 870–881 (2014).

38. 38.

Harabagiu, V., Pinteala, M., Cotzur, C., Holerca, M. N. & Ropot, M. Functional Polysiloxanes. 3. Reaction of 1,3-Bis(3-Glycidoxypropyl)−1,1,3,3-Tetramethyldisiloxane with Amino Compounds. J. Macromol. Sci. A 32, 1641–1648 (1995).

39. 39.

Hutchins, K. M. Functional materials based on molecules with hydrogen-bonding ability: applications to drug co-crystals and polymer complexes. R. Soc. Open Sci. 5, 180564 (2018).

40. 40.

El Wakkad, S. E. S. & Shams El Din, A. M. The anodic oxidation of metals at very low current density. Part V. Gold. J. Chem. Soc., 3098–3102, https://doi.org/10.1039/JR9540003098 (1954).

41. 41.

Zhang, W., Bas, A. D., Ghali, E. & Choi, Y. Passive behavior of gold in sulfuric acid medium. Trans. Nonferrous Met. Soc. China 25, 2037–2046 (2015).

42. 42.

Petrović, Ž., Metikoš-Huković, M., Babić, R., Katić, J. & Milun, M. A multi-technique study of gold oxidation and semiconducting properties of the compact α-oxide layer. J. Electroanal. Chem. 629, 43–49 (2009).

43. 43.

Gnapareddy, B. et al. Chemical and physical characteristics of doxorubicin hydrochloride drug-doped salmon DNA thin films. Sci. Rep. 5, 12722 (2015).

44. 44.

Depan, D. & Misra, R. D. K. Hybrid nanostructured drug carrier with tunable and controlled drug release. Mater. Sci. Eng. C. 32, 1704–1709 (2012).

45. 45.

Szajdzinska-Pietek, E., Pinteala, M. & Schlick, S. Monitoring pH-dependent conformational changes in aqueous solutions of poly(methacrylic acid)-b-polydimethylsiloxane copolymer based on fluorescence spectra of pyrene and 1,3-bis(1-pyrenyl)propane. Polymer 45, 4113–4120 (2004).

46. 46.

Victor, S. P., Paul, W., Jayabalan, M. & Sharma, C. P. Supramolecular hydroxyapatite complexes as theranostic near-infrared luminescent drug carriers. CrystEngComm 16, 9033–9042 (2014).

47. 47.

López-García, J., Lehocký, M., Humpolíček, P. & Sáha, P. HaCaT keratinocytes response on antimicrobial atelocollagen substrates: extent of cytotoxicity, cell viability and proliferation. J. Funct. Biomater. 5, 43–57 (2014).

48. 48.

Böhmová, E. et al. Cell-penetrating peptides: a useful tool for the delivery of various cargoes into cells. Physiol. Res. 67, S267–S279 (2018).

49. 49.

Turkevich, J., Stevenson, P. C. & Hillier, J. A study of the nucleation and growth processes in the synthesis of colloidal gold. Discuss. Faraday Soc. 11, 55–75, https://doi.org/10.1039/DF9511100055 (1951).

## Acknowledgements

This research was financially supported by the Internal Research Grant Nr. 30886/30.12.2014, Project Manager: Raoul-Vasile Lupuşoru, MD, PhD (at “Grigore T. Popa” University of Medicine and Pharmacy, Iasi, Romania) and JINR projects 04-4-1121/2017, items 39 and 62; the grant of the Romanian National Authority for Scientific Research and Innovation, CNCS/CCCDI – UEFISCDI, project number PN-III-P1-1.2-PCCDI-2017-0697, within PNCDI III program; and the project that has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No. 667387 WIDESPREAD 2-2014 SupraChem Lab.

## Author information

Authors

### Contributions

All the authors contributed to the submitted manuscript and are aware of this submission. R.V.L., D.A.P., C.M.U, A.C., C.S., M.P. designed research and supervised the project. R.V.L., D.A.P., C.M.U., M.P. provided funding acquisition, project administration, and resources. R.V.L., D.A.P., C.M.U., A.A., A.C., I.E., M.F.Z., M.S. performed the synthesis, analysis and processed the data. All authors discussed the results and contributed to the original draft. C.M.U., A.C., M.P. review and edit the final version of the manuscript.

### Corresponding authors

Correspondence to Cristina M. Uritu or Adina Coroaba or Mariana Pinteala.

## Ethics declarations

### Competing interests

The authors declare no competing interests.

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

## Rights and permissions

Reprints and Permissions

Lupusoru, R., Pricop, D.A., Uritu, C.M. et al. Effect of TAT-DOX-PEG irradiated gold nanoparticles conjugates on human osteosarcoma cells. Sci Rep 10, 6591 (2020). https://doi.org/10.1038/s41598-020-63245-8

• Accepted:

• Published: