Clinical motor and non-motor effects of deep brain stimulation (DBS) of the subthalamic nucleus (STN) in Parkinson's disease (PD) seem to depend on the stimulation site within the STN. We analysed the effects of the position of the stimulation electrode within the motor STN on subjective emotional experience, expressed as emotional valence and arousal ratings to pictures representing primary rewards and aversive fearful stimuli in 20 PD patients. Patients’ ratings from both aversive and erotic stimuli matched the mean ratings from a group of 20 control subjects at similar position within the STN. Patients with electrodes located more posteriorly reported both valence and arousal ratings from both the rewarding and aversive pictures as more extreme. Moreover, posterior electrode positions were associated with a higher occurrence of depression at a long-term follow-up. This brain–behavior relationship suggests a complex emotion topography in the motor part of the STN. Both valence and arousal representations overlapped and were uniformly arranged anterior-posteriorly in a gradient-like manner, suggesting a specific spatial organization needed for the coding of the motivational salience of the stimuli. This finding is relevant for our understanding of neuropsychiatric side effects in STN DBS and potentially for optimal electrode placement.
Perception and experience of emotion in humans seem to be implemented by a range of cortical and subcortical regions involved in large-scale brain networks according to evidence from numerous neuroimaging studies1,2. However, neuroimaging methods are inherently limited by low spatial and temporal resolution in comparison with invasive techniques recording local field potentials or single-unit activity. Since the opportunities to use such techniques in humans are rare, mapping of neural representation of emotion on a small-scale remains highly fragmented.
Deep brain stimulation (DBS) of the subthalamic nucleus (STN), which has become a standard treatment for motor complications in Parkinson's disease (PD)3,4 has provided a unique opportunity to study the functional architecture of the basal ganglia in humans. Besides motor control and action selection, the basal ganglia have an important role in decision-making, emotion, language, learning, memory, and more5. Current knowledge of the functional organization of frontal cortico-basal ganglia network highlights the integration of information from functionally distinct cortical regions in the target basal ganglia (i.e. the striatum and the STN) established by a convergence of cortical projections to create zones of unique combinations of afferents6,7,8,9,10,11,12,13. These, in turn, may have unique functions7.
In line with findings of functional overlap of the dorsolateral motor, central associative, and ventromedial limbic territory of the STN14, we recently identified single-neurons responding to emotional stimuli in the dorsolateral motor territory of the STN in PD patients undergoing STN DBS15. At the same time, clinical motor and non-motor effects of DBS seem to depend on the electrode position within the STN in a manner that reflects the functional organization and connectivity of the STN16,17,18,19. Surprisingly, despite the high rate of neuropsychiatric side effects and changes in emotional processes found in patients successfully treated by STN DBS for motor complications20, the effect of the electrode position on subjective emotional experience has not been studied so far.
Emotional behaviour is organized along two psycho-physiological dimensions: emotional valence, varying from negative to neutral to positive, and arousal, varying from low to high21,22. Previously, we found the effects of the STN DBS on subjective valence and arousal ratings from affective pictures representing primary rewards and aversive fearful stimuli. With the DBS switched ON, aversive fearful stimuli were discerned as more negative according to their valence ratings23.
Considering the functional anatomy of the STN, in this study we further analysed the relationship between the electrode position within the STN and individual affective ratings in PD patients treated with bilateral STN DBS. Emotional valence and arousal ratings from pictures representing primary rewards (visual sexual stimuli) and aversive fearful stimuli were recorded postoperatively in OFF-medicated PD patients with the stimulation ON and OFF and in matched control subjects. Coordinates of each active electrode contact and anatomical delineation of each individual STN were determined from a pre- and postoperative MRI and averaged from both hemispheres. Each STN was transformed into the standardized stereotactic space (Montreal Neurological Institute – MNI)24 and both the active electrode contacts and the positions of the neurons responding to either emotional valence or arousal from our previous study were plotted together to compare the functional anatomy of emotional valence and arousal on large and small scales.
Electrode position and affective ratings
The positions of the active electrode contacts within the STN plotted in the standardized stereotactic MNI space with the delineation of the motor, associative and limbic region are shown in Fig. 1. The position of the active electrode contacts within the STN varied from y = −16.5 mm to y = −10.7 in antero-posterior direction, from x = 11.0 mm to x = 15.5 mm in medio-lateral direction, and from z = −9.7 to −4.3 mm in dorso-ventral direction. However, only the positions along the antero-posterior axis were associated with a gradient-like organization reflecting emotional ratings.
The valence ratings of erotic stimuli were gradually affected by the active electrode contact position. The more posterior the contact position, the higher the valence of erotic stimuli (χ2(1) = 9.69, P = 0.0019) (Fig. 2a, top). This gradient, defined as the change in affective rating per unit length, did not differ between the DBS conditions (χ2(1) = 0.90, P = 0.3431). The equality point, defined as the position along the antero-posterior axis in which patients, on average, rated the valence equally to control subjects, was at y = −12.3 mm for erotic pictures. On average, the valence increased by 0.36 on the valence scale for each 1 mm of contact shift in the posterior direction.
Similarly, valence ratings to aversive stimuli were also affected by the active electrode contact position. The more posterior the contact position, the lower the valence of aversive pictures (χ2(1) = 12.7, P = 0.0004) (Fig. 2a, bottom) showing a 0.37 decrease with each 1 mm contact shift in the posterior direction. This gradient also did not differ between the DBS conditions (χ2(1) = 1.87, P = 0.1715), and the equality point was similar in both DBS OFF (y = −12.6 mm) and ON (y = −12.2 mm) conditions.
The arousal ratings of erotic stimuli also correlated with the active electrode contact position. The more posterior the contact position, the higher the erotic arousal (χ2(1) = 5.43, P = 0.0198) (Fig. 2b). This represents a gradient of 0.60 increase for each 1 mm of contact shift in the posterior direction with the equality point located at y = −11.0 mm. This gradient did not differ between DBS conditions (χ2(1) = 1.95, P = 0.3767).
In aversive stimuli, arousal ratings did not seem to correlate with the active electrode contact position (χ2(1) = 2.71, P = 0.0998). However, this negative result could be explained by two outlying patients (#3 and #19) who rated the arousal of both erotic and aversive stimuli as relatively low (Fig. 2b,c). Omitting these outliers, an antero-posterior gradient in aversive arousal ratings could be seen as well. The more posterior the contact position, the higher the aversive arousal was (χ2(1) = 5.01, P = 0.0251) (Fig. 2b) showing 0.34 increase per 1 mm of contact shift with no difference between the DBS conditions (χ2(1) = 0.0034, P = 0.9533) and with the equality point located at y = −8.8 mm.
Spatial organization of valence and arousal gradients
The spatial organization of the emotional gradients, i.e. the direction and magnitude of the relative change in the affective ratings per 1 mm in the antero-posterior direction, was similar for the valence ratings from both erotic and aversive stimuli and for arousal ratings from erotic stimuli (Fig. 3). Additionally, the antero-posterior position in which patients rated pictures similar to healthy controls (the equality point) was similar for these emotional dimensions. However, the effect of the electrode position on valence ratings was stronger than on arousal ratings, which is consistent with our previous findings23.
The emotional gradients described above were found with respect to the position of the electrode contact computed as the average from the left and right active electrode contacts of the bilateral stimulation. This approach could confound the precise examination of emotional changes. Thus, to assess the potential impact of this averaging procedure, we studied the emotional ratings in respect to the position of the left and right active electrode contacts separately and continued to find similar gradients when considering the left and right contacts separately (Appendix Figs. A1, A2, Table A1).
Acute effects of subthalamic stimulation: DBS ON vs. OFF comparison
Motor involvement assessed by the motor score of the Unified Parkinson's Disease Rating Scale (UPDRS III) score decreased from 40.2 (SD 10.5) in the DBS OFF condition to 17.8 (6.6) in the DBS ON condition (t(19) = 11.9, P <0.0001). This decrease in UPDRS III did not correlate with the active electrode contact position (F(3,16) = 0.69, P = 0.57) in any direction.
The comparative analysis of DBS ON vs. OFF conditions on emotional assessment showed a significant difference in valence ratings for aversive stimuli only. In the DBS ON condition, patients rated the valence of aversive pictures lower (on average by 0.17, t(19) = −3.21, P = 0.0046) compared to the OFF condition. However, there was no difference in the arousal ratings of aversive stimuli (t(19) = 1.19, P = 0.25). Also, valence and arousal ratings of erotic stimuli did not differ between DBS conditions. Thus, we reproduced our previous results suggesting an increased activation of the aversive motivational system and attributing to a higher salience to aversive stimuli due to STN DBS23,25. Interestingly, the size of the DBS-related decrease in the valence ratings of aversive stimuli did not depend on the electrode position. Moreover, this acute DBS effect on aversive valence ratings (i.e., lowering by 0.17) was an order of magnitude weaker compared to the strong chronic effect of the electrode position (i.e., lowering by 2.2, considering the most anterior and the most posterior positions of the active electrode contact).
We investigated the possibility that the changes to the affective ratings were related to the surgical intervention itself, i.e. due to a STN lesion caused by the insertion of the DBS electrode and/or its pure presence. We found similar position-related effects even after adjusting for the STN lesion volume. At the same time, the ratings variability could be better explained in terms of the active electrode contact position than the electrode tip position (see Appendix B for details).
Psychiatric long-term outcome analysis
While only one patient suffered from depression preoperatively, 11 patients developed depressive and/or anxiety disorder during the follow-up period (mean follow-up was 11.1 y (SD 2.7 y, range 5–16 y). The average time from the operation to the diagnosis of depression was 4.7 y (SD 3.7 y). In 3 out of the 4 patients who were diagnosed with depression within the first year after surgery, the onset was clearly related to a drastic reduction of dopaminergic treatment by more than 75%.
Most interestingly, all eight patients with the electrode located within the posterior part of the STN (further than 13.9 mm behind the anterior commissure) had developed depression at the long-term follow-up. On the other hand, only 3 out of the 12 patients with an electrode located more anteriorly developed depression. Notably, the electrode position could predict the occurrence of later depression in our patient sample. On average, the shift in the electrode contact of 1 mm in the posterior direction was associated with increased odds to depression by 390% (χ2 = 6.78, P = 0.0092) adjusted for the preoperative BDI score.
In contrast, six patients suffered from a psychotic complication during the follow-up period. Three patients had their electrode located within the posterior part of the STN and three in the anterior part. The electrode position could not predict the occurrence of psychosis (χ2 = 2.79, P = 0.0948, adjusting for the preoperative BDI score).
Our results provide evidence for the effects of the electrode position within the motor part the STN on the subjective emotional experience of patients with PD treated with chronic bilateral DBS. Irrespective of whether the DBS was turned ON or OFF, patients with more posterior active electrode contacts reported both valence and arousal ratings from both rewarding and aversive pictures as more extreme than patients with contacts located anteriorly. Therefore, as both the valence and arousal representations seem to be uniformly arranged along the antero-posterior direction and spatially overlap, this gradient-like organization could represent a general code for stimulus motivational salience within the dorsolateral STN26.
The electrode position affecting the subjective experience of emotional pictures was within the motor region and was accompanied by excellent effects of DBS on motor symptoms. None of our patients had the active electrode contact placed in the limbic part of the STN which had previously been associated with acute emotion and mood changes27,28,29,30.This finding further demonstrates the functional overlap of motor and non-motor regions in the STN and supports its key role in emotional processes and integrative function14,20.
The electrode position-related effect on the subjective emotional experience was present in both the DBS ON and the OFF conditions which could be a consequence of several mechanisms: (i) surgical intervention (i.e. due to potential damage to the STN parenchyma caused by the insertion of the DBS electrode as already suggested by verbal fluency impairment31), or (ii) due to the long-term structural effects of the DBS electrodes within the brain parenchyma (i.e. axonal, glial and reactive inflammatory changes as evidenced by several histopathological autopsy studies on brains of DBS patients32,33), or (iii) due to the long-term effects of chronic STN DBS (i.e. persisting after-effect of DBS of the stimulated volume)34,35. We found similar position-related effects even after adjusting for the STN lesioned volume, and a weaker position-related effect when considering the tip of the electrode instead of the active contact suggesting that the position of the electrode itself within the STN and/or the long-term effects of chronic STN DBS are more likely to explain our results. DBS induces hierarchical and temporally disparate changes across the brain that in some cases parallel the latencies of improvement in symptoms upon turning the stimulator on and the worsening of symptoms after turning the stimulator off35. The lack of differentiation between gradients in the DBS ON and OFF conditions on the one hand and the lack of position-related effects on motor symptoms on the other could also be explained by a hypothetical instant spread of acute stimulation to distances much larger than the range of the active electrode contact positions, which would be manifested in position-non-specific acute effects.
The only change in affective ratings due to the stimulation switched ON was a decrease in valence to aversive pictures which is in agreement with our previous work suggesting that the acute STN DBS drives the affective ratings away from the normative ratings and activates the aversive motivational system23,25. However, OFF-medication related distress with a bias towards aversive stimuli could also interfere with the affective ratings. This could potentiate the aversive activation due to the stimulation switched ON while masking some subtle counter-acting DBS induced changes in the subjective experience of the appetitive stimuli. Interestingly, acute changes were relatively weak as compared to the effects of the electrode position within the STN.
Two important types of signals relate to emotionally charged stimuli - the value and the salience signals. Each has distinct roles in motivational control and have been linked to distinctive neural circuits26,36, which can be differentiated in our study design (Fig. 4). Value signals quantify how good or bad something is, and is important for seeking goals, evaluation of outcomes, value learning, and decision making26. On the other hand, salience signals convey information about the stimulus's significance, and are important for orienting attention, arousal, enhancement of cognitive processing, immediate behavioural reaction, and general motivational drive26. Salience is low for non-rewarding and non-aversive stimuli and high for both rewarding and aversive stimuli.
In this study, we found a position-dependent gradual change of emotional ratings from moderate valence and low arousal in the anterior part of the motor STN to extreme valence and high arousal in its posterior part, which is indicative of salience representation in the motor region of the STN. Conversely, a representation of the stimulus value would have been represented by a gradual position-dependent change of valence from very negative in one part, neutral in the middle, and very positive in another part of the STN.
Indeed, several lines of evidence from both animal and human studies have indicated the involvement of the STN in salience attribution20,37,38. Within the frontal cortical areas, such as the anterior cingulate gyrus and the anterior insula, a number of studies have revealed a distributed neural representation of motivational salience as organized gradients for both rewards and punishments36,39,40. Thus, the direct topographically organized and overlapping cortical projections from these regions to the STN can provide the basis for conveying various motivational properties of a stimulus in an organized gradient like-manner to the nucleus for integration and subcortical representation of salience.14,41 Within the basal ganglia, the STN is under a strong influence from both the external pallidum, which receives several inputs from limbic structures, such as the amygdala, the paraventricular nucleus of the hypothalamus, the ventral pallidum41,42,43, and midbrain dopaminergic neurons. Dopamine signalling has long been recognized as an essential contributor to coding of a reward-prediction-error and incentive salience processing in the basal ganglia26,44.
Following our previous findings of subthalamic affective neurons45, this study further extends evidence for a topographic organization of neural maps of the emotional dimensions of valence and arousal within the same anatomical space. While microelectrode recordings identified two spatially segregated neuronal populations responding either to emotional valence or arousal, suggesting their separate processing at a small-scale level, the macroelectrode-related effects demonstrated a smooth gradient-like organization of these two emotional dimensions on a large-scale level within the same anatomical region. Such a two-level organization provides evidence for both segregation and integration of these emotional dimensions within the STN. The interaction between multiple small maps located within larger maps may be complex and may support the emergence of new response properties46. Similar to our findings, different large-scale and small-scale organizations have been found in visual and auditory system studies that have often revealed a heterogeneous functional micro-organization embedded in the smooth macroscale order. Topographic maps with different scales recur throughout the nervous system. They seem to play a role in higher-level cognition enabling a rapid and faithful relay of information between the two regions47 and as is now suggested by our study, including brain systems coding motivational value and salience. Conceivably, the topographic organization in the STN might overlap with other types of organizations such as convergence or divergence in addition to numerous other forms of connectivity45.
However, inferences drawn from the functional anatomy of the STN have to be taken with caution because the study was conducted with PD patients, who are known to suffer from a widespread central nervous system pathology48 and experience problems with emotional processing49. Furthermore, the presence of the gradient in the STN is not necessarily emotion-specific. It could just be a consequence of selectively increased susceptibility of the posterior part of the STN to the active presence of a DBS electrode with the possible roles of lesion, plasticity, or persisting DBS after-effects. Alternatively, the effects on emotional processing could be mediated by the spread of stimulation to structures posterior to the STN, i.e. the zona incerta50,51. However, as compared to the STN DBS no psychiatric side-effects were associated with DBS of the zona incerta52,53,54,55. Moreover, evidence indicates that stimulation effects are also due to axonal modulation and affection of a fibre network56. The hyperdirect cortico-subthalamic fibres from the dorsolateral prefrontal and orbitofrontal cortex and cingulate gyrus and the pallidothalamic fibres are located dorsally and posteriorly to the STN and the observed effects on emotional processing could be related to the stimulation of these tracts7,57.
Integrative subthalamic hubs and their projections could be important sites of abnormal long-term plasticity due to DBS and may be linked to long-term psychiatric outcomes in STN DBS treated patients resulting in postoperative depression or psychosis. The frequency of postoperative depression requiring antidepressant treatment is usually high in these patients (up to 60% during the follow-up period of 10 years, similarly to our findings)20,58 and the origin seem to be heterogeneous. A reduction of dopaminergic treatment within the first year after surgery59 may explain a severe depression only in tree patients in our study. As previously suggested postoperative depression was not found to be related to electrode location during the first year after the STN DBS surgery19,60. However, our data suggest that posterior electrode placement within the motor part of the STN might be associated with an increased risk of depression during later periods. In contrast, psychosis frequency was proportionate to the PD duration and did not depend on electrode position which also fit with the hypothesis that the topography found in our study is solely emotional.
As the variability in electrode position was not related to the extent of motor symptom improvement, the more anterior electrode placement in the anterior part of the STN might be associated with good clinical motor outcomes without negative effects on emotion processing and depression development.
This study on the impact of STN DBS electrode position on emotional experience provides evidence for a causal brain–emotional behaviour relationship. Valence and arousal representations seem to be organized along the antero-posterior direction within the motor part of the STN.
The finding of more extreme ratings from both rewarding and aversive stimuli suggests the attribution of higher emotional salience to all emotional stimuli perceived under DBS in the posterior part of the STN. While there was no impact of the electrode position on motor symptom reduction, more posterior active electrode contacts were associated with more extreme ratings of emotional stimuli compared to control subjects and poorer long-term psychiatric outcomes due to a higher occurrence of depression.
This finding is highly relevant for clinical decisions on electrode placement as well as for the understanding of the mechanisms underlying emotion dysregulation reported in the DBS STN.
Materials and Methods
Twenty PD patients (all males; mean (SD) age 58.4 (6.0) y, range 45.6–67.0 y; mean disease duration 15.3 (4.4) y), range 9–26 y; treated with bilateral STN DBS for motor fluctuations and/or dyskinesias (mean STN DBS duration 2.2 (1.5) y, range 0.4–6.0 y) and 21 control subjects (all males; mean (SD) age 56.1 (6.5) y, range 39.5–67.1 y); were included in the study. All the patients fulfilled the UK Brain Bank Criteria for the diagnosis of PD61.
The study was approved by the local Ethics Committee of the General University Hospital in Prague and all participants gave their informed consent prior to being included in the study. The methods were carried out in accordance with the relevant guidelines and regulations.
On the day of the study all participants were screened for cognitive and mood status using the Mini Mental State Examination (MMSE)62 and the Beck Depression inventory (BDI)63. The patients’ demographic variables and disease characteristics are summarized in Table 1. No differences were found for age, MMSE, BDI or education duration between the patients and control group. In the PD group, the mean daily dose of dopaminergic medications (in levodopa equivalents64 was 684 (SD 532) mg, range 250–2250 mg). Four patients were on long-term antidepressant therapy (1 on citalopram, 1 on mirtazapine, 1 on sertraline, 1 on a combination of escitalopram and mirtazapine). One of the control subjects was on anxiolytic therapy with buspirone. No other psychotropic medication was taken. All patients were chronically stimulated by bilateral monopolar STN DBS. The mean levodopa change was 51% of the preoperative dose (range 18–120%). After the surgery all patients had follow-up visits in our Movement Disorders Centre. We recorded the data on postoperative psychiatric outcomes: (i) the presence of clinical depression that required an initiation of treatment with antidepressants, the year of depression onset, the pre-operative levodopa equivalent, and the levodopa equivalent at the time of depression onset, and (ii) the presence of psychosis, and the year of psychosis onset.
Affective task and procedure
Twenty-one pictures representing visual sexual stimuli (erotic females and couples) and 21 aversive pictures representing victims (mutilations) and threat (human or animal attacks, aimed guns) selected from the International Affective Picture system (IAPS)65 were rated during the emotional task. The numbers of the selected pictures are presented in the Appendix C1.
Patients were tested after withdrawing from dopaminergic medication overnight. The last medication dose had been administered no later than at 8 p.m. the day before testing, so that the average time the patients were off medication was at least 16.3 hours (min 11.9, max 19.5 h). On the day of testing their stimulators were switched OFF for 2 hours starting at 8 a.m. Then they were tested in the two conditions with the STN DBS switched ON and OFF in counterbalanced orders. There was a 1-hour break between when the stimulators were switched into the particular condition and affective testing (thus the stimulators had been switched OFF for 3 hours in patients who were tested in the OFF condition first). Pictures were presented to PD patients in different orders in the DBS ON and DBS OFF conditions. In each condition prior to affective testing the Unified Parkinson's disease rating scale (UPDRS III) rating was performed by a rater who was unaware of the DBS condition.
Each picture was presented on a touch-sensitive screen for a period of 6 s. As described previously, subjects were required to rate each picture separately along the dimension of emotional valence and arousal on two independent visual scales25. Valence was rated on a 1–9 scale, with 1 being the most negative, 5 being neutral, and 9 being the most positive (i.e. lower valence for aversive stimuli means a more aversive/negative assessment). Arousal was rated on a 1–9 scale, with 1 being the calmest and 9 being the most arousing.
Mapping of the electrode positions
The position of the active electrode contacts were determined as described previously45: First, both the frameless preoperative 3.0 Tesla T2-weighted (T2w) MRI visualizing the STN, and the frameless postoperative 1.5 Tesla T1-weighted (T1w) MRI displaying the susceptibility artifact of the permanent DBS electrode were automatically co-registered with the frame-based preoperative 1.5 Tesla T1w MRI used for neurosurgical planning in each patient using the SurgiPlan software (Elekta, Stockholm, Sweden), thereby getting all preoperative and postoperative images for each patient into individual stereotactic space. The frameless postoperative 1.5 Tesla T1w MRI was acquired approximately 2–5 days after DBS implantation (MP-RAGE, i.e. Magnetization-Prepared Rapid Gradient Echo sequence, 160 axial slices with a spatial resolution of 0.9 × 0.9 × 1.65 mm, TR = 2140 ms, TE = 3.93 ms, FA = 15 degrees). The position of the active electrode contact in each STN (defined as x, y, z coordinates in mm) was reconstructed based on the location of the T1w MRI susceptibility artifact of the permanent DBS electrode using SurgiPlan software. Then, to get the active contacts from the STN of all patients into one reference space, twelve points delineating anatomically each individual STN and 2 additional points – the anterior commissure (AC) and the posterior commissure (PC) - were identified manually in axial and coronal projections in the preoperative 3 Tesla T2w MRI (28 axial slices and 21 coronal slices, 2 mm thick, with x-y resolution 0.9 × 0.9 mm; TR = 2430 ms, TE = 80 ms). The delineating points were co-registered with the 14 corresponding points of the reference model that consisted of coronal slices of the STN manually digitized from the atlas by Morel24 using a linear mapping approach that involved shifting, scaling and rotation. In each patient we averaged the positions of the active electrode contacts from both hemispheres as a common electrode contact defined in the STN model as the point lying halfway between the right active electrode contact and the left active electrode contact mirrored to the right hemisphere. The mean distance between such pairs of the active electrode contacts was 2.5 (SD 1.1) mm (range 1.0 to 5.0 mm).
Finally, we performed another linear transformation of the Morel atlas into standardized stereotactic MNI 152 ICBM space66 used by the Lead-DBS tool67, in which visualization in context of the Ewert atlas68 was carried out. We found optimal transformation parameters (shifting, rotation and scaling) that minimized the sum of square distances between all points on the coronal slices of the Morel atlas and the surface of the Ewert atlas. All rotations were below 13° (αx = 0.6°, αy = −11.1°, αz = −13.0°) and scaling was below 30% (sx = 92.9%, sy = 77.7%, sz = 128.4%).
The difference in the UPDRS III score in the ON and OFF conditions was assessed using the Student's paired t-test. The difference in the affective ratings of individual stimuli between DBS ON and OFF conditions was assessed using linear mixed effects models. To capture the repeated nature of ratings specific to patients and stimuli, one random effect was included for each picture, one for each patient in each DBS condition, and one for each combination of picture and patient.
To assess the spatial gradient in the affective ratings, we fitted linear mixed-effects models that attempted to explain the ratings of individual stimuli in terms of the fixed effects of the active electrode contact position along the medio-lateral, antero-posterior, and dorso-ventral directions, DBS condition (ON or OFF), and two covariates to adjust for the patients’ post-operative depressive condition: the BDI mood status and the indication of any anti-depressive therapy (which was uncorrelated with the mood status). Random effects were included for each picture, for each patient in each DBS condition, and for each picture-patient pair. To identify the simplest, yet well-fitting model, we successively eliminated individual spatial directions from the models and stopped when the P-value of the model-submodel likelihood ratio test was less than 0.1, which suggested that the submodel, having the direction removed, could not fit the data as well as the model including the direction. To assess the spatial gradient in motor symptom reduction, we related the DBS OFF-ON difference in the UPDRS III score to the three spatial directions using a linear model. To assess the influence of the active contact position on the postoperative psychiatric outcomes, we used a logistic regression model with an additional covariate of preoperative BDI to adjust for the preoperative mood status. Statistical analyses were carried out in R69.
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
Lindquist, K. A. & Barrett, L. F. A functional architecture of the human brain: emerging insights from the science of emotion. Trends Cogn Sci 16, 533–540, https://doi.org/10.1016/j.tics.2012.09.005 (2012).
Tamietto, M. & de Gelder, B. Neural bases of the non-conscious perception of emotional signals. Nat Rev Neurosci 11, 697–709, https://doi.org/10.1038/nrn2889 (2010).
Limousin, P. et al. Electrical stimulation of the subthalamic nucleus in advanced Parkinson's disease. N Engl J Med 339, 1105–1111, https://doi.org/10.1056/NEJM199810153391603 (1998).
Schuepbach, W. M. et al. Neurostimulation for Parkinson's disease with early motor complications. N Engl J Med 368, 610–622, https://doi.org/10.1056/NEJMoa1205158 (2013).
Eisinger, R. S., Urdaneta, M. E., Foote, K. D., Okun, M. S. & Gunduz, A. Non-motor Characterization of the Basal Ganglia: Evidence From Human and Non-human Primate Electrophysiology. Front Neurosci 12, 385, https://doi.org/10.3389/fnins.2018.00385 (2018).
Haber, S. N., Kim, K. S., Mailly, P. & Calzavara, R. Reward-related cortical inputs define a large striatal region in primates that interface with associative cortical connections, providing a substrate for incentive-based learning. J Neurosci 26, 8368–8376, https://doi.org/10.1523/JNEUROSCI.0271-06.2006 (2006).
Heilbronner, S. R., Meyer, M. A. A., Choi, E. Y. & Haber, S. N. How do cortico-striatal projections impact on downstream pallidal circuitry? Brain Struct Funct 223, 2809–2821, https://doi.org/10.1007/s00429-018-1662-9 (2018).
Calzavara, R., Mailly, P. & Haber, S. N. Relationship between the corticostriatal terminals from areas 9 and 46, and those from area 8A, dorsal and rostral premotor cortex and area 24c: an anatomical substrate for cognition to action. Eur J Neurosci 26, 2005–2024, https://doi.org/10.1111/j.1460-9568.2007.05825.x (2007).
Draganski, B. et al. Evidence for segregated and integrative connectivity patterns in the human Basal Ganglia. J Neurosci 28, 7143–7152, https://doi.org/10.1523/JNEUROSCI.1486-08.2008 (2008).
Averbeck, B. B., Lehman, J., Jacobson, M. & Haber, S. N. Estimates of projection overlap and zones of convergence within frontal-striatal circuits. J Neurosci 34, 9497–9505, https://doi.org/10.1523/JNEUROSCI.5806-12.2014 (2014).
Choi, E. Y., Tanimura, Y., Vage, P. R., Yates, E. H. & Haber, S. N. Convergence of prefrontal and parietal anatomical projections in a connectional hub in the striatum. Neuroimage 146, 821–832, https://doi.org/10.1016/j.neuroimage.2016.09.037 (2017).
Mailly, P., Aliane, V., Groenewegen, H. J., Haber, S. N. & Deniau, J. M. The rat prefrontostriatal system analyzed in 3D: evidence for multiple interacting functional units. J Neurosci 33, 5718–5727, https://doi.org/10.1523/JNEUROSCI.5248-12.2013 (2013).
Yeterian, E. H. & Van Hoesen, G. W. Cortico-striate projections in the rhesus monkey: the organization of certain cortico-caudate connections. Brain Res 139, 43–63, https://doi.org/10.1016/0006-8993(78)90059-8 (1978).
Haynes, W. I. & Haber, S. N. The organization of prefrontal-subthalamic inputs in primates provides an anatomical substrate for both functional specificity and integration: implications for Basal Ganglia models and deep brain stimulation. J Neurosci 33, 4804–4814, https://doi.org/10.1523/JNEUROSCI.4674-12.2013 (2013).
Sieger, T. et al. Distinct populations of neurons respond to emotional valence and arousal in the human subthalamic nucleus. Proc Natl Acad Sci USA 112, 3116–3121, https://doi.org/10.1073/pnas.1410709112 (2015).
Herzog, J. et al. Most effective stimulation site in subthalamic deep brain stimulation for Parkinson's disease. Mov Disord 19, 1050–1054, https://doi.org/10.1002/mds.20056 (2004).
Ruzicka, F. et al. Chronic stress-like syndrome as a consequence of medial site subthalamic stimulation in Parkinson's disease. Psychoneuroendocrinology 52, 302–310, https://doi.org/10.1016/j.psyneuen.2014.12.001 (2015).
Ruzicka, F. et al. Weight gain is associated with medial contact site of subthalamic stimulation in Parkinson's disease. PLoS One 7, e38020, https://doi.org/10.1371/journal.pone.0038020 (2012).
Welter, M. L. et al. Optimal target localization for subthalamic stimulation in patients with Parkinson disease. Neurology 82, 1352–1361, https://doi.org/10.1212/WNL.0000000000000315 (2014).
Castrioto, A., Lhommee, E., Moro, E. & Krack, P. Mood and behavioural effects of subthalamic stimulation in Parkinson's disease. Lancet Neurol 13, 287–305, https://doi.org/10.1016/S1474-4422(13)70294-1 (2014).
Lang, P. J., Greenwald, M. K., Bradley, M. M. & Hamm, A. O. Looking at pictures: affective, facial, visceral, and behavioral reactions. Psychophysiology 30, 261–273 (1993).
Russell, J. A. Core affect and the psychological construction of emotion. Psychol Rev 110, 145–172 (2003).
Serranova, T. et al. Subthalamic nucleus stimulation affects incentive salience attribution in Parkinson's disease. Mov Disord 26, 2260–2266, https://doi.org/10.1002/mds.23880 (2011).
Morel, A. Atlas of the Human Thalamus and Basal Ganglia. 160, Informa Helthcare (2007).
Serranova, T. et al. Sex, Food and Threat: Startling Changes After Subthalamic Stimulation in Parkinson's Disease. Brain Stimul, https://doi.org/10.1016/j.brs.2013.03.009 (2013).
Bromberg-Martin, E. S., Matsumoto, M. & Hikosaka, O. Dopamine in motivational control: rewarding, aversive, and alerting. Neuron 68, 815–834, https://doi.org/10.1016/j.neuron.2010.11.022 (2010).
Mallet, L. et al. Stimulation of subterritories of the subthalamic nucleus reveals its role in the integration of the emotional and motor aspects of behavior. Proc Natl Acad Sci USA 104, 10661–10666, https://doi.org/10.1073/pnas.0610849104 (2007).
Ulla, M. et al. Contact dependent reproducible hypomania induced by deep brain stimulation in Parkinson's disease: clinical, anatomical and functional imaging study. J. Neurol. Neurosurg. Psychiatry 82, 607–614, https://doi.org/10.1136/jnnp.2009.199323 (2011).
Krack, P. et al. Mirthful laughter induced by subthalamic nucleus stimulation. Mov. Disord. 16, 867–875 (2001).
Polosan, M. et al. Affective modulation of the associative-limbic subthalamic nucleus: deep brain stimulation in obsessive–compulsive disorder. Translational Psychiatry 9, 73, https://doi.org/10.1038/s41398-019-0404-y (2019).
Witt, K. et al. Relation of lead trajectory and electrode position to neuropsychological outcomes of subthalamic neurostimulation in Parkinson's disease: results from a randomized trial. Brain 136, 2109–2119, https://doi.org/10.1093/brain/awt151 (2013).
Kronenbuerger, M. et al. Brain alterations with deep brain stimulation: New insight from a neuropathological case series. Mov Disord 30, 1125–1130, https://doi.org/10.1002/mds.26247 (2015).
DiLorenzo, D. J., Jankovic, J., Simpson, R. K., Takei, H. & Powell, S. Z. Neurohistopathological findings at the electrode-tissue interface in long-term deep brain stimulation: systematic literature review, case report, and assessment of stimulation threshold safety. Neuromodulation 17, 405–418; discussion 418, https://doi.org/10.1111/ner.12192 (2014).
Jech, R. et al. The subthalamic microlesion story in Parkinson's disease: electrode insertion-related motor improvement with relative cortico-subcortical hypoactivation in fMRI. PLoS One 7, e49056, https://doi.org/10.1371/journal.pone.0049056 (2012).
Agnesi, F., Johnson, M. D. & Vitek, J. L. Deep brain stimulation: how does it work? Handb Clin Neurol 116, 39–54, https://doi.org/10.1016/B978-0-444-53497-2.00004-8 (2013).
Zhang, Z. et al. Distributed neural representation of saliency controlled value and category during anticipation of rewards and punishments. Nat Commun 8, 1907, https://doi.org/10.1038/s41467-017-02080-4 (2017).
Uslaner, J. M., Dell’Orco, J. M., Pevzner, A. & Robinson, T. E. The influence of subthalamic nucleus lesions on sign-tracking to stimuli paired with food and drug rewards: facilitation of incentive salience attribution? Neuropsychopharmacology 33, 2352–2361, https://doi.org/10.1038/sj.npp.1301653 (2008).
Baunez, C., Yelnik, J. & Mallet, L. Six questions on the subthalamic nucleus: lessons from animal models and from stimulated patients. Neuroscience 198, 193–204, https://doi.org/10.1016/j.neuroscience.2011.09.059 (2011).
Grabenhorst, F. & Rolls, E. T. Value, pleasure and choice in the ventral prefrontal cortex. Trends Cogn Sci 15, 56–67, https://doi.org/10.1016/j.tics.2010.12.004 (2011).
Kringelbach, M. L. The human orbitofrontal cortex: linking reward to hedonic experience. Nat Rev Neurosci 6, 691–702, https://doi.org/10.1038/nrn1747 (2005).
Parent, A. & Hazrati, L. N. Functional anatomy of the basal ganglia. II. The place of subthalamic nucleus and external pallidum in basal ganglia circuitry. Brain Res Brain Res Rev 20, 128–154 (1995).
Haber, S. N. & Knutson, B. The reward circuit: linking primate anatomy and human imaging. Neuropsychopharmacology 35, 4–26, https://doi.org/10.1038/npp.2009.129 (2010).
Hunt, A. J. Jr. et al. Paraventricular hypothalamic and amygdalar CRF neurons synapse in the external globus pallidus. Brain Struct Funct 223, 2685–2698, https://doi.org/10.1007/s00429-018-1652-y (2018).
Berridge, K. C. & Robinson, T. E. What is the role of dopamine in reward: hedonic impact, reward learning, or incentive salience? Brain Res Brain Res Rev 28, 309–369 (1998).
Sieger, T. et al. Basal ganglia neuronal activity during scanning eye movements in Parkinson's disease. PLoS One 8, e78581, https://doi.org/10.1371/journal.pone.0078581 (2013).
Rothschild, G. & Mizrahi, A. Global order and local disorder in brain maps. Annu Rev Neurosci 38, 247–268, https://doi.org/10.1146/annurev-neuro-071013-014038 (2015).
Thivierge, J. P. & Marcus, G. F. The topographic brain: from neural connectivity to cognition. Trends Neurosci 30, 251–259, https://doi.org/10.1016/j.tins.2007.04.004 (2007).
Braak, H. et al. Staging of the intracerebral inclusion body pathology associated with idiopathic Parkinson's disease (preclinical and clinical stages). J Neurol 249(Suppl 3), III/1-5 (2002).
Peron, J., Dondaine, T., Le Jeune, F., Grandjean, D. & Verin, M. Emotional processing in Parkinson's disease: a systematic review. Mov Disord 27, 186–199, https://doi.org/10.1002/mds.24025 (2012).
Parent, A. & Hazrati, L. N. Functional anatomy of the basal ganglia. I. The cortico-basal ganglia-thalamo-cortical loop. Brain Res Brain Res Rev 20, 91–127 (1995).
Schaltenbrand, G., Wahren, W. & Hassler, R. Atlas for Stereotaxy of the Human Brain. (Thieme, 1977).
Castrioto, A. et al. Iowa gambling task impairment in Parkinson's disease can be normalised by reduction of dopaminergic medication after subthalamic stimulation. J Neurol Neurosurg Psychiatry, https://doi.org/10.1136/jnnp-2013-307146 (2014).
Plaha, P., Ben-Shlomo, Y., Patel, N. K. & Gill, S. S. Stimulation of the caudal zona incerta is superior to stimulation of the subthalamic nucleus in improving contralateral parkinsonism. Brain 129, 1732–1747, https://doi.org/10.1093/brain/awl127 (2006).
de Chazeron, I. et al. Impact of localisation of deep brain stimulation electrodes on motor and neurobehavioural outcomes in Parkinson's disease. J Neurol Neurosurg Psychiatry 87, 758–766, https://doi.org/10.1136/jnnp-2015-310953 (2016).
Chou, X. L. et al. Inhibitory gain modulation of defense behaviors by zona incerta. Nat Commun 9, 1151, https://doi.org/10.1038/s41467-018-03581-6 (2018).
Hamani, C. et al. Subthalamic Nucleus Deep Brain Stimulation: Basic Concepts and Novel Perspectives. eNeuro 4, https://doi.org/10.1523/ENEURO.0140-17.2017 (2017).
Gallay, M. N., Jeanmonod, D., Liu, J. & Morel, A. Human pallidothalamic and cerebellothalamic tracts: anatomical basis for functional stereotactic neurosurgery. Brain Struct Funct 212, 443–463, https://doi.org/10.1007/s00429-007-0170-0 (2008).
Bang Henriksen, M. et al. Surviving 10 years with deep brain stimulation for Parkinson's disease—a follow-up of 79 patients. Eur. J. Neurol. 23, 53–61, https://doi.org/10.1111/ene.12614 (2016).
Thobois, S. et al. Non-motor dopamine withdrawal syndrome after surgery for Parkinson's disease: predictors and underlying mesolimbic denervation. Brain 133, 1111–1127, https://doi.org/10.1093/brain/awq032 (2010).
Perriol, M. P. et al. Stimulation of the subthalamic nucleus in Parkinson's disease: cognitive and affective changes are not linked to the motor outcome. Parkinsonism Relat. Disord. 12, 205–210, https://doi.org/10.1016/j.parkreldis.2005.11.009 (2006).
Hughes, A. J., Daniel, S. E., Kilford, L. & Lees, A. J. Accuracy of clinical diagnosis of idiopathic Parkinson's disease: a clinico-pathological study of 100 cases. J Neurol Neurosurg Psychiatry 55, 181–184 (1992).
Folstein, M. F., Folstein, S. E. & McHugh, P. R. Mini-mental state”. A practical method for grading the cognitive state of patients for the clinician. J Psychiatr Res. 12, 189–198 (1975).
Beck, A., Steer, R. & Brown, G. The Beck depression inventory-II. (Psychological Corporation, 1996).
Kleiner-Fisman, G. et al. Subthalamic nucleus deep brain stimulation: summary and meta-analysis of outcomes. Mov Disord 21(Suppl 14), S290–304 (2006).
Lang, P. J. & Bradley, M. M., & Cuthbert, B. N. International affective picture system (IAPS): Affective ratings of pictures and instruction manual. Technical Report A-8. University of Florida, Florida, Gainesville, FL. (2008).
Fonov, V. et al. Unbiased average age-appropriate atlases for pediatric studies. Neuroimage 54, 313–327, https://doi.org/10.1016/j.neuroimage.2010.07.033 (2011).
Horn, A. & Kuhn, A. A. Lead-DBS: a toolbox for deep brain stimulation electrode localizations and visualizations. Neuroimage 107, 127–135, https://doi.org/10.1016/j.neuroimage.2014.12.002 (2015).
Ewert, S. et al. Toward defining deep brain stimulation targets in MNI space: A subcortical atlas based on multimodal MRI, histology and structural connectivity. Neuroimage. https://doi.org/10.1016/j.neuroimage.2017.05.015 (2017).
R: A language and environment for statistical computing (R Foundation for Statistical Computing, Viena, Austria, 2017).
Accolla, E. A. et al. Brain tissue properties differentiate between motor and limbic basal ganglia circuits. Hum. Brain Mapp. 35, 5083–5092, https://doi.org/10.1002/hbm.22533 (2014).
Ewert, S. et al. Toward defining deep brain stimulation targets in MNI space: A subcortical atlas based on multimodal MRI, histology and structural connectivity. Neuroimage 170, 271–282, https://doi.org/10.1016/j.neuroimage.2017.05.015 (2018).
Bradley, M. M. & Lang, P. J. Measuring emotion: the Self-Assessment Manikin and the Semantic Differential. J. Behav. Ther. Exp. Psychiatry 25, 49–59 (1994).
Lang, P. J. Behavioral treatment and bio-behavioral assessment: Computer applications. In Technology in mental health care delivery systems (eds. Sidowski, J. B., Johnson, J. H. & Williams, T. A.), 119–137 (Norwood, N. J. Ablex, 1980).
Supported by a grant from the Czech Science Foundation (GAČR 16-13323S) and Czech Ministry of Health, grant no. AZV 16-29651A and NV19-04-00233, and Progres Q27/LF1. All rights reserved. We would like to thank Markéta Fialová, Martin Voleman, and Anna Rezková for administrative and technical support.
The authors declare no competing interests.
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Serranová, T., Sieger, T., Růžička, F. et al. Topography of emotional valence and arousal within the motor part of the subthalamic nucleus in Parkinson’s disease. Sci Rep 9, 19924 (2019). https://doi.org/10.1038/s41598-019-56260-x
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