Optimization and characterization of Royal Dawn cherry (Prunus avium) phenolics extraction

Abstract

To correlate the beneficial effects of cherry consumption with their phenolic composition, a full and precise characterization is required. However, there is not a specific method to fully extract all phenolic compounds from sweet cherries. Thus, this study aimed to optimize the extraction of sweet cherry phenolics by response surface methodology and fully characterize the phenolic profile of Royal Dawn sweet cherries by HPLC-ESI-MS/MS. Extraction conditions were evaluated and optimized to 55 °C, MeOH 72%, 12 mL/g in two extraction steps. Royal Dawn sweet cherries presented rutin as the predominant phenolic compound, unlike most sweet cherry varieties. Additionally, ethanol was evaluated as a replacement solvent, obtaining lower extraction rates, especially for anthocyanins. However, in terms of total amounts, non-anthocyanin compounds were similarly extracted. The developed methodology was fast and can be routinely used in the evaluation of the phenolic profile of sweet cherries and to produce phenolic-rich extracts for the food industry.

Introduction

Cherries are known for their wide range of bioactive compounds, including phenolic compounds1. The phenolic profile of sweet cherries has been widely studied2,3,4,5. Sweet cherries are rich in anthocyanins, hydroxycinnamic acids, flavonols and flavan-3-ols2,3,4,5. In sweet cherries, anthocyanins occur mostly as cyanidin-3-O-rutinoside2,6, while hydroxycinnamic acids occur mostly as chlorogenic and neochlorogenic acids3,6,7. Flavonols occur mainly as rutin2,5, and flavan-3-ols as epicatechin and catechin2,3,4.

Importantly, sweet cherry consumption has been associated with several beneficial effects1. To correlate its consumption with health effects, proper characterization of the phenolic profile is required. To do so, specific methodologies to fully extract phenolic compounds are necessary. In this sense, extraction factors such as temperature, liquid-to-solid ratio (LSR), solvent, and time influence the extraction of phenolic compounds from anthocyanin-rich fruits8,9. In the specific case of sweet cherries, several extraction parameters vary widely between studies6,10,11,12. The wide variability of extraction methods2,4,5,6,11,12 makes it controversial to compare the phenolic profile of sweet cherries among studies.

Considering the chemical complexity and variety of phenolic compounds present in fruits and vegetables13, as well as the factors that potentially can affect the extraction process14, it becomes difficult to develop a universal extraction method for all food matrices14. Hence, the optimization of the extraction of phenolic compounds in different food matrices is essential. In this sense, response surface methodology (RSM) has been effective to optimize polyphenols extraction from different plant materials8,15,16,17,18, including phenolic compounds from sour cherry pomace8,19. Although the phenolic profile of sour cherries is similar to the one reported for sweet cherries, relevant differences exist20. In this sense, the most abundant anthocyanin and flavonol in sour cherries are cyanidin-3-O-glucosyl-rutinoside and kaempferol-3-O-rutinoside, respectively12,20,21. Moreover, sour cherries have reported a higher total phenolic content (TPC) than that of sweet cherries20,22 as well as different sugar and protein contents22. This evidence suggests that the optimal conditions for the extraction of sweet and sour cherry phenolic compounds might differ.

To our knowledge, the only optimized extraction method for sweet cherry phenolics has been recently developed by Blackhall et al.23. However, this method was developed only to extract anthocyanins, while other relevant phenolic compounds were not considered. Indeed, the optimal extraction conditions depend on the type of phenolic compound18. Thus, to date, no specific methods that aim to fully extract all phenolic compounds from sweet cherries exist. Therefore, this study aimed to apply RSM to develop an extraction method that can be used to extract all phenolics present in sweet cherry varieties, and to characterize the phenolic profile of Royal Dawn sweet cherry by HPLC-ESI-MS/MS for the first time.

Results and Discussion

Sweet cherries are a rich source of phenolic compounds with relevant biological activities1,2. Specific methods that fully extract phenolic compounds for each food matrix are required to completely characterize these compounds and to link food consumption with a health benefit. Methods have been developed for the extraction of anthocyanins in Lapins sweet cherries23 and anthocyanin-rich fruits18,23, and phenolic compounds from sour cherry pomace8,19. However, to our knowledge, no methods that aim to fully extract the most representative phenolic families of sweet cherry varieties exist. Therefore, in this study, we investigated the factors affecting sweet cherry phenolics extraction and optimized them to develop an extraction method useful in sweet cherry varieties. Specifically, the LSR, solvent percentage and extraction temperature were optimized though RSM, while extraction time and number of extractions were evaluated by classical one-variable-at-a-time approach. Methanol (MeOH) was selected as the extraction solvent thought the optimization steps of this study due to its higher extraction rate of phenolic compounds than other organic solvents14,16,18,24. In fact, once optimized, the extraction method was used to completely characterize by HPLC-ESI-MS/MS the phenolic profile of Royal Dawn sweet cherries for the first time. Moreover, considering the application of extraction methodologies to produce phenolic-rich extracts with potential bioactivities, ethanol (EtOH) was evaluated as MeOH replacement extraction solvent due to MeOH toxicity and prohibited use for food industry’s purposes14.

Response surface methodology

The extraction of sweet cherry phenolics was optimized using the RSM approach previously used by Yılmaz et al. in sour cherries8. However, sour cherries matrix differ considerably to sweet cherries such as their most abundant phenolic compound12,20,21. Extraction time (30 min) was fixed during the RSM experiment in line with other studies in the literature18,23. The TPC, total anthocyanin content (TAC) and anthocyanins, hydroxycinnamic acids and flavonols quantified by HPLC-DAD were included in the RSM so as to predict the extraction conditions that are optimal for the most relevant phenolic families present in sweet cherries2,3. The experimental results for all runs were included in the model (Table 1).

Table 1 Rotatable central settings of independent variables and experimental results of total polyphenols content (TPC), total anthocyanins content (TAC), Cy3R (cyanidin-3-o-rutinoside), hydroxycinnamic acids (HCA) and flavonols (FO).

Fitting the model

The experimental data (Table 1) were used to determine the regression coefficients of Eq. (1). All the selected compounds generated a significant model, confirming that at least one of the extraction variables could explain the variation of the response variable in comparison with its mean. The coefficients of determination (R2) and p-values for the lack of fit test can be found in Table 2.

Table 2 Analysis of variance and regression coefficients of predicted model for response variables in sweet cherries.

Analysis of regression coefficients

A significant (p < 0.05) positive linear effect of MeOH was found for TAC, Cy3R and FO, while a tendency (p < 0.1) was observed for TPC, indicating that an increase in MeOH increases the extraction of those compounds. Linear models have also been reported in the extraction of flavan-3-ols in different plant matrices16. A tendency (p < 0.1) towards negative quadratic MeOH effects was observed for the TAC, implying that its extraction increases up to an optimal MeOH percentage after which it starts to decrease (Fig. 1). Positive linear and negative quadratic effects of the extraction solvent are found for the extraction of TAC in sour cherries8.

Figure 1
figure1

Response surface plots for total polyphenols content (TPC; a), total anthocyanins content (TAC; b,c), cyanidin-3-O-rutinoside (Cy3R; d), hydroxycinnamic acids (HCA; e) and flavonols (FO; f) of sweet cherries as a function of extraction temperature, methanol proportion and liquid-to-solid ratio (LSR). A at MeOH = 50%, B at LSR = 6 mL/g; and (cf at T = 40 °C.

No significant linear or quadratic effects were observed for TAC, Cy3R, HCA or FO, which is in disagreement with different studies in different stone and anthocyanin-rich fruits8,17. In agreement with our results, Ku et al.25 did not report a significant effect of temperature on the extraction of anthocyanins from Rubus coreanus marc. Temperature only produced a positive quadratic effect on the extraction of TPC. Similarly, the extraction of TPC, tartaric esters and flavonols from black currants was not influenced by the extraction temperature evaluated in a very similar range to our study9. These result suggest that the effect of temperature on the extraction of sweet cherry phenolics is not very relevant (Fig. 1), and this could be due to the maintenance of extraction temperature below 65 °C to avoid phenolics degradation8.

TPC, HCA and FO presented a significant positive linear effect of LSR and TAC a significant positive quadratic effect, which implies that a higher LSR will result in a higher extraction of these compounds (Fig. 1). Our results are in agreement with the extraction of different phenolic compounds from sour cherries8 and other plant matrices9,16,25. A significant interaction effect between MeOH and LSR was observed for the extraction of TAC and Cy3R, which was negative in both cases, implying that, depending on the MeOH proportion, the LSR has a different effect. Although crossover effects are not common in the literature, several studies report them16,25.

Validation of the model

The combination of extraction variables at the highest desirability (0.801) was selected to optimize the extraction method. Specifically, this corresponded to 55 °C; 72% MeOH and 12 mL/g; three extractions were performed under those conditions to confirm the model’s prediction (Table 3). No differences were obtained between the predicted and experimental values of TAC, Cy3R, HCA and FO, which confirmed the model’s accuracy. However, the TPC values were outside the range predicted by the model. However, obtaining a higher TPC than that predicted does not represent a serious drawback, as our goal was to extract the maximum phenolic compounds. Therefore, extraction temperature, MeOH concentration and LSR were fixed at 55 °C, 72% and 12 mL/g throughout the rest of the study. Surprisingly, the optimized LSR was the same as that reported for sour cherry phenolics extraction8 and was very similar to the one reported in the extraction of anthocyanins from Lapins sweet cherries23. Despite that, the MeOH concentration and extraction temperature were significantly different8,23.

Table 3 Overall optimal extraction parameters for phenolic compounds in sweet cherries.

Effect of time on phenolic extraction

Changes in the response variables due to the effect of time are shown in Table 4. Although different studies report a significant effect of time in anthocyanin-rich fruits8,9,16,17,18,23, in our study, no significant differences were reported due to the effect of extraction time. The fact that phenolic compounds are rapidly transferred into the extraction solvent makes our method more economically feasible than the methods developed for sour cherry phenolics (100 min) and sweet cherry anthocyanins (90 min)8,23. However, our results suggest that the solvent is saturated right after the sample extraction solvent are mixed, opening the door to the study of successive extractions.

Table 4 Effect of time on the extraction of sweet cherry phenolic compounds.

Effect of multiple-step extractions on phenolic extraction

Multi-step extractions are a useful strategy to increase the extraction yield of phenolic compounds in food matrices15. The results show a considerable increase in the extraction of phenolic compounds between the first and second extraction steps (Fig. 2). However, after the second extraction step, no significant increases were found, indicating that the extraction is mostly completed at the second extraction step. Therefore, two sequential steps were defined as optimal and used throughout the rest of the experiment.

Figure 2
figure2

Effect of sequential extraction on the extraction of total polyphenols content (TPC; a), total anthocyanins content (TAC; b), cyanidin-3-O-rutinoside (Cy3R; c), hydroxycinnamic acids (HCA; d) and flavonols (FO; e) from sweet cherries. The results are expressed as milligrams of phenolic equivalent per gram of dry weigh ± SD (n = 3) and percentage. Different letters (one-way ANOVA, Tukey’s test) indicate significant differences between extraction steps.

Phenolic profile of royal dawn sweet cherries by HPLC-ESI-MS/MS

The phenolic profile of Royal Dawn sweet cherries by HPLC-ESI-MS/MS (Table 5) is in agreement with the major phenolic families occurring in other sweet cherries2,3,4,5. Cyanidin-based anthocyanins were found to be predominant, and cyanidin-3-O-rutinoside was the anthocyanin with the highest concentration, which is consistent with the literature2,3,10,12. Several hydroxycinnamic acids were found in high concentrations in this study, which is consistent with the fact that stone fruits are rich in these type of phenolic compounds7. Indeed, caffeoylquinic acid derivatives are widely reported among the phenolic compounds with the highest concentration in sweet cherries. Rutin was found at a higher concentration than that of any other compound in our study, and although rutin is reported as the main flavonol in sweet cherries2,5, only few varieties report it as the predominant phenolic compound6,10. The main flavan-3-ol representative in Royal Dawn sweet cherries was epicatechin, which had a 3-fold higher concentration than that of catechin and this follows the general trend that epicatechin is more concentrated than catechin in sweet cherries2,5. Procyanidin dimer B2 was the procyanidin with the highest concentration and reached higher concentrations than those of catechin. Although not common, this trend has been observed in other varieties such as Beritello sweet cherries5.

Table 5 Phenolic compounds of sweet cherry extracted using methanol (MeOH) or ethanol (EtOH) as extraction solvents.

Investigation of solvent replacement

The solvent EtOH was included in the study to evaluate the potential of the developed method to generate phenolic-rich extracts for the food industry. The extraction conditions were the same as the optimized in MeOH (two consecutive extractions, 55 °C, 72% and 12 mL/g). The methanolic and ethanolic extracts of sweet cherries showed that, in general, phenolic compounds were better extracted in MeOH than they were in EtOH (Table 5), which is consistent with the literature14,16,18,24. In the specific case of anthocyanins, methanolic extraction achieved significantly higher yield, which were also relevant in terms of total amounts. Only a few anthocyanins (i.e., delphinidin O-coumaroylglucose d1) were extracted at higher amounts in the ethanol-based extraction. Consistent with our results, MeOH was a better extraction solvent for anthocyanins in blueberries24. For the non-anthocyanin compounds, MeOH based-extraction only achieved statistically significant and relevant higher extraction rates (>20%) of ferulic acid, quercetin-O-glucoside, isorhamnetin-3-O-glucoside, procyanidin dimer d3 and procyanidin trimer. For the ethanol-based extraction, only protocatechuic acid and quercetin, which were significantly extracted in higher amounts with EtOH, reached a relevant increase (>20%) of their concentration. Our results are in agreement with other studies that evaluate the extraction of non-anthocyanin phenolic compounds in sour cherry pomace8. With the exception of anthocyanins, relevant sweet cherry phenolics with potential bioactivities10,13, such as rutin or procyanidin dimer B2, were similarly extracted in both extraction solvents. Consequently, the adaptation of ethanol-based extraction to the food industry could still be useful to produce phenolic extracts with potential health bioactive effects. Additionally, the use of MeOH-based methodology can be used to routinely characterize phenolic profiles from sweet cherries.

We optimized by RSM a specific method to rapidly extract all phenolic compounds from sweet cherries. Additionally, we used the optimized method to fully extract and correctly profile by HPLC-ESI-MS/MS the phenolic composition of Royal Dawn sweet cherries and demonstrated that, unlike most sweet cherry varieties, rutin is the predominant phenolic compound. This methodology could be routinely used to extract phenolics from sweet cherries for their full characterization. This characterization is essential to link cherry fruit consumption health-promoting effects with their phenolic profile. Moreover, this method could be applied to produce phenolic-rich extracts for the food industry.

Materials and Methods

Plant material

Royal Dawn sweet cherries (Prunus avium) were purchased from Mercabarna (Barcelona, Spain) and were originally from Mendoza (Argentina). Cherry stones were manually removed and flesh was frozen in liquid nitrogen and grounded. Next, homogenates were lyophilized for a week in a Telstar LyoQuest lyophilizer (Thermo Fisher Scientific, Madrid, Spain) at −55 °C and ground to a fine homogeneous powder using a conventional chopping machine (Moulinette 1, 2, 3, Moulinex) which was kept dry and protected from humidity and light exposure until extraction.

Chemicals and reagents

All water used in this study was ultrapure water, which was obtained from a Milli-Q Advantage A10 system (Madrid, Spain). The organic solvents used for the HPLC analyses and the extraction of phenolic compounds from sweet cherries (acetonitrile, ethanol and methanol) as well as glacial acetic acid were all HPLC analytical grade and were purchased from Panreac (Barcelona, Spain). Formic acid was purchased from Scharlab (Barcelona, Spain). The Folin-Ciocalteu reagent was purchased from Fluka/Sigma-Aldrich (Madrid, Spain). The standard compounds apigenin, chlorogenic acid, eriodictyol, eriodyctiol-7-O-glucoside, hyperoside (quercetin-3-O-glucoside), isorhamnetin, isorhamnetin-3-O-glucoside, kaempferol, kaempferol-3-O-glucoside, and kaempferol-3-O-rutinoside were purchased from Extrasynthese (Lyon, France). The standard compounds benzoic acid, caffeic acid, (+)-catechin, epigallocatechin gallate (EGCG), p-coumaric acid, (−)-epicatechin, ferulic acid, gallic acid, phloroglucinol, procyanidin dimer B2, protocatechuic acid and quercetin were purchased from Fluka/Sigma-Aldrich (Barcelona, Spain). The standard anthocyanin compounds cyanidin-3-O-rutinoside, malvidin-3-O-glucoside and peonidin-3-O-rutinoside were purchased from PhytoLab (Vestenbergsgreuth, Germany). Resveratrol was purchased from Quimivita (Barcelona, Spain), and rutin was kindly provided by Nutrafur (Murcia, Spain).

To conduct this study, all non-anthocyanidin standard compounds were dissolved individually in MeOH at 2 mg/mL, with the exception of isorhamnetin-3-O-glucoside (1 mg/mL) and hyperoside (0.5 mg/mL). Anthocyanidin standard compounds (cyanidin-3-O-rutinoside, malvidin-3-O-glucoside and peonidin-3-O-rutinoside) were dissolved individually in MeOH (0.01% HCl) at 0.5 mg/mL. These standard stock solutions were stored in amber glass flasks at −20 °C and prepared newly when older than 3 month and used to construct calibration curves for polyphenols quantification.

Extraction procedure

Cherry powder was weighed to obtain the desired LSR and mixed with 1.5 mL of pre-heated extraction solvent (methanol:water, v:v). Different extraction MeOH concentrations, extraction temperatures, times and extraction steps were used throughout the experiment. MeOH was prepared in all cases including 1% formic acid to promote plant’s matrix degradation16. Extractions were performed in 2 mL Eppendorf tubes in a shaking and heating plate (Thermo Fischer Scientific, Madrid, Spain) at 500 rpm agitation under protection from light exposure and then samples were centrifuged at 9,500 g for 10 min at 4 °C. Supernatants were stored at −20 °C until further analyses.

Response surface design

The extraction of sweet cherry phenolics was optimized using an experimental design by RSM8. A rotatable central composite design with three factors and five levels was selected. The design consisted of 17 randomized runs with three center point replicates. The independent variables used were temperature (T, X1; 15–65 °C), methanol concentration (MeOH, X2; methanol:water, 0–100%) and LSR (X3; 4–14 mL/g). Extraction time (30 min) was fixed as a constant during the RSM experiment. Experimental data were fitted to a second polynomial response surface, which follows the equation:

$$y={\beta }_{0}+{\sum }_{i=1}^{k}{\beta }_{i}{X}_{i}+{\sum }_{i=1}^{k}{\beta }_{ii}{X}_{i}^{2}+{\sum }_{\begin{array}{c}i=1\\ i < j\end{array}}^{k-1}{\sum }_{j=2}^{k}{\beta }_{ij}{X}_{i}{X}_{j}$$
(1)

where Y is the dependent variable, β0 the constant coefficient, and βi, βii and βij are the linear, quadratic and interaction regression coefficients, respectively. Xi, Xii and Xij represent the independent variables. Independent variables included generic determinations and individual compounds detected by HPLC-DAD. The results of the RSM design were analyzed with Design-expert 9.0.6 software (Trial version, Stat-Ease Inc., Minneapolis, MN, USA).

Kinetic study

A kinetic study was performed to evaluate the effect of time on the polyphenols extraction yield in sweet cherries. Seven extraction times from 0 to 120 min were selected. The LSR was fixed at 12 mL/g, MeOH percentage at 72% and temperature at 55 °C. The TPC, TAC and anthocyanins, hydroxycinnamic acids and flavonols quantified by HPLC-DAD were used to evaluate the effect of time on polyphenols extractability.

Effect of multi-step extractions

Four consecutive extractions were performed in order to evaluate the influence of multiple extractions on polyphenols extraction yield in sweet cherries. Samples were mixed with the pre-heated (55 °C) extraction solvent (MeOH of 72%) in a LSR of 12 mL/g and immediately centrifuged (9,500 × g, 10 min, 4 °C). Pellets were re-extracted under the same extraction conditions three more times, and supernatants were collected again and stored for polyphenols content analyses. The TPC, TAC and anthocyanins, hydroxycinnamic acids and flavonols quantified by HPLC-DAD were used to evaluate the effect of sequential extractions on the polyphenols extraction yield.

Phenolic characterization of sweet cherries

Sweet cherry phenolic profile was accurately quantified in methanol- and an ethanol-based (EtOH) extractions. Briefly, samples were mixed with the pre-heated (55 °C) extraction solvent (MeOH or EtOH of 72% including 1% formic acid) in a LSR of 12 mL/g and immediately centrifuged (9,500 g, 10 min, 4 °C). This procedure was conducted twice, and supernatants were recollected and analyzed. The characterization of sweet cherries was performed by the developed HPLC-ESI-MS/MS methodology.

Analysis of response variables

Total polyphenol and anthocyanin contents

The TPC and TAC of cherry extracts were determined by the Folin-Ciocalteu and pH differential methods from Iglesias-Carres et al.18. The results were expressed as milligram of gallic acid or cyanidin-3-O-rutinoside equivalent per gram of dry weight (mg GAE or Cy3R/g dw). The molar absorbance of Cy3R (595.2 g/mol) used was 28,800 L/mol × cm.

HPLC-DAD and HPLC-ESI-MS/MS quantification of phenolic compounds

In the RSM study, the detection and quantification of sweet cherry phenolics was performed by HPLC-DAD in the same system and conditions developed in Iglesias-Carres et al.18. Method quality parameters can be found in S1 Table.

In the HPLC-ESI-MS/MS quantification system, the extracts were directly analyzed using a 1200 LC Series coupled to a 6410 MS/MS (Agilent Technologies, Palo Alto, CA, USA). Of note, two different HPLC-ESI-MS/MS systems were used to separate, detect and quantify non-anthocyanin and anthocyanin phenolic compounds. Non-anthocyanin compounds separation was achieved using a ZORBAX Eclipse XDB-C18 (150 mm × 2.1 mm i.d., 5 µm particle size) as the chromatographic column equipped with a Narrow-Bore guard column (2.1 mm × 12.5 mm, 5 µm particle size) (Agilent Technologies, Palo Alto, CA, USA) as previously described in Iglesias-Carres et al.18. Separation of anthocyanins was achieved using an Acquity BHE C18 column (50 mm × 2.1 mm, 5 µm particle size) (Waters, Milford, MA, USA) as previously described in Iglesias-Carres et al.18. Optimized conditions for the analysis of non-anthocyanin and anthocyanin phenolic compounds are summarized in S2 Table. In both methodologies, data acquisition was carried out using MassHunter Software (Agilent Technologies, Palo Alto, CA, USA). The calibration curves, coefficient of determination, linearity and detection and quantification limits for non-anthocyanin and anthocyanin phenolic compounds can be found in S3 Table.

Statistical analysis

All experiments carried out thought this manuscript were performed in triplicates. Design-expert 9.0.6 software (Trial version, Stat-Ease Inc., Minneapolis, MN, USA) was used to analyze the results of the RSM section. For any other statistical analysis SPSS 19 software (SPSS Inc., Chicago, IL, USA) was used. The statistics’ significance was evaluated using a one-way ANOVA (Tukey’s test) or Student’s t-test, and statistical significance was considered when p < 0.05.

Abbreviations

Cy3R, cyanidin-3-O-rutinoside; dw, dry weigh; EtOH, ethanol; FO, flavonols; GAE, gallic acid equivalents; HCA, hydroxycinnamic acids; LSR, liquid-to-solid ratio; MeOH, methanol; T, temperature; TAC, total anthocyanin content; and TPC, total polyphenol content.

Data availability

All data generated or analyzed during this study are included in this published article (and its Supplementary Information Files).

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Acknowledgements

This work was supported by the Spanish Ministry of Economy and Competitiveness (grant numbers AGL2013-49500-EXP, AGL2016-77105-R) and by European Regional Development Funds of the European Union within the Operative Program FEDER of Catalunya 2014–2020 (NUTRISALT). L.I.-C. is recipient of a predoctoral fellowship from Universitat Rovira i Virgili – Martí i Franquès. Grant number: 2015PMF-PIPF-50. A.M.-C. is recipient of a predoctoral fellowship from Universitat Rovira i Virgili – Martí i Franquès. Grant number: 2015PMF-PIPF-51. A. A.-A. and F.I.B. are Serra Húnter fellows. Grant numbers URV-LE-621 and URV-AG-587. We express deep thanks Dr. Cinta Bladé for the conception of this project and Dr. Pol Herrero, Dr. Antoni Del Pino, Niurka Llópiz and Rosa M. Pastor for their technical help and advice.

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F.I.B., M.M., A.A.-A. and B.M. designed and directed the research; L.I.-C. and A.M.C. performed the research; L.I.-C., A.A.-A. and B.M. wrote the paper; B.M. achieved the funding. All authors discussed the results and implications and commented on the manuscript at all stages. All authors have read and approved the final manuscript.

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Correspondence to Begoña Muguerza.

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Iglesias-Carres, L., Mas-Capdevila, A., Bravo, F.I. et al. Optimization and characterization of Royal Dawn cherry (Prunus avium) phenolics extraction. Sci Rep 9, 17626 (2019). https://doi.org/10.1038/s41598-019-54134-w

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