Placental extravillous trophoblast (EVT) invasion is essential in establishing proper blood supply to the fetus during pregnancy. However, traditional 2D in vitro systems do not model the in vivo invasion process in an anatomically-relevant manner. Our objectives were to develop a 3D spheroid model that would allow better emulation of placental invasion in vitro and to characterize the transcriptomic and functional outcomes. HTR8/SVneo EVT cells were self-assembled into 3D spheroids using ultra-low attachment plates. Transcriptomic profiling followed by gene set enrichment and gene ontology analyses revealed major global transcriptomic differences, with significant up-regulations in EVTs cultured as 3D spheroids in canonical pathways and biological processes such as immune response, angiogenesis, response to stimulus, wound healing, and others. These findings were further validated by RT-qPCR, showing significant up-regulations in genes and/or proteins related to epithelial-mesenchymal transition, cell-cell contact, angiogenesis, and invasion/migration. A high-throughput, spheroid invasion assay was applied to reveal the dynamic invasion of EVTs away from the spheroid core into extracellular matrix. Lastly, lipopolysaccharide, dexamethasone, or Δ9-tetrahydrocannabinol exposure was found to impact the invasion of EVT spheroids. Altogether, we present a well-characterized, 3D spheroid model of EVT invasion and demonstrate its potential use in drug and toxin screening during pregnancy.
The human placenta is a transient organ that forms the interface between the mother and fetus during pregnancy1. One of its key functions during early pregnancy is invasion and migration into the maternal decidua2,3. Proper invasion is critical to the remodeling of maternal spiral arteries into high capacitance, low resistance vessels that supply oxygen and nutrients to the fetus4. The primary placental cell type responsible for regulating invasion is the extravillous trophoblast (EVT), which bears the ability to migrate away from the solid trophoblast columns of the anchoring chorionic villi and invade into the maternal decidua5. This process is highly regulated by a variety of physiochemical factors (e.g. oxygen, growth factors, nutrients, extracellular matrix proteins), and dysregulated EVT invasion can conversely result in compromised placentation and an inability to properly support both the mother and fetus6. Interestingly, pregnancy-related diseases like preeclampsia and placenta accreta have been strongly associated with altered EVT invasion and spiral artery remodeling, both poorly understood pathological outcomes of impaired trophoblast function6. However, our ability to better understand the cellular and molecular underpinnings of these placental diseases may be limited, in part, by the unrepresentative nature of current two-dimensional (2D) in vitro systems.
Accumulating evidence in other organ systems and cancer models suggest that culturing cells in three-dimensions (3D) can provide more anatomically- and physiologically-relevant results compared to traditional 2D monolayer cultures7. While some researchers have begun to culture placental cells as 3D spheroids or organoids8,9,10,11,12, our overall understanding of the transcriptomic changes and functional outcomes associated with its formation remains preliminary. Characterization studies contrasting novel 3D spheroids against 2D monolayers are especially lacking – yet, these are necessary to justify its use and advancement, as the theorized advantages (e.g., enhanced cell-cell contact, physiological function, etc.7) are not verified in placental in vitro cultures. Moreover, there are no spheroid models to date that directly and specifically mimic the trophoblast column of the anchoring chorionic villi, and demonstrate functional EVT invasion and migration away from its core as seen in vivo13. To address this, we develop a self-assembling, 3D spheroid culture system of the trophoblast column and EVT invasion, and use a transcriptome-wide microarray and bioinformatics approach to characterize the global gene expression profiles against traditional 2D monolayers. We further apply a high-throughput, spheroid invasion assay to measure the actual invasion of the 3D EVT spheroids into extracellular matrix in real-time, and assess the impact of various drugs and compounds.
Extravillous trophoblast cells self-assembled into 3D spheroids with high viability over eight days
HTR8/SVneo EVT cells seeded in ultra-low attachment plates at densities of 1,000, 5,000, and 10,000 cells/mL all self-assembled into spheroids within two days with average diameters of 211.1 µm, 323.7 µm, and 411.6 µm, respectively (Fig. 1a). Spheroid diameter increased at rates of 36.05 ± 2.01, 31.26 ± 1.73 and 26.65 ± 3.47 µm/day over eight days, respective to the seeding densities, and maintained structural integrity throughout (Fig. 1a,b). Spheroids seeded at 5,000 cells/mL were used in subsequent experiments as they maintained mean diameters of less than 500 µm over eight days, above which has been frequently characterized with necrotic cores14. Indeed, live and dead staining using calcein AM and ethidium homodimer-1, respectively, confirmed that spheroids maintain high viability (98.9–99.9% live cells) over eight days (Fig. 1c,d). Hematoxylin and eosin (H&E) staining of cross-sections also revealed that the spheroids exhibit solid cores (Fig. 1e), resembling the solid structural anatomy of trophoblast columns in vivo15.
Distinct transcriptomic profiles in EVTs cultured as 3D spheroids and 2D monolayers
To understand how global gene expression profiles were changed in EVTs cultured as 3D spheroids compared to 2D monolayers, we analyzed 21,448 genes via Clariom S Human transcriptome profiling microarray (Thermo). A total of 4,562 gene probe sets were found to be differentially expressed in 3D spheroids compared to 2D monolayers, with 2,327 genes up-regulated and 2,235 genes down-regulated (FDR p-value < 0.05; absolute fold change ≥2; Fig. 2a). Cluster dendrogram analyses demonstrated distinct grouping between samples from 3D spheroid and 2D monolayer groups and a large height distance to convergence, suggesting highly different global transcriptome profiles between the two groups (Fig. 2b). The short height distance to convergence seen for samples within each of the two groups suggests similar transcriptome profiles within each group (Fig. 2b).
Gene set enrichment analysis of canonical pathways and biological processes
We next performed gene set enrichment analysis (GSEA) on the transcriptome microarray data to identify the enriched canonical pathways in extravillous trophoblasts cultured as 3D spheroid compared to 2D monolayers (Fig. 3a). The top fifteen most significantly enriched canonical pathway terms in EVTs cultured as 3D spheroids included categories: “neuroactive ligand-receptor interaction”, “immune processes, diseases, defensins”, “signaling events”, and “ECM” (FDR p < 0.05; Supplementary Table S1). The top fifteen most significantly down-regulated pathway terms in 3D spheroids included categories: “diseases”, “transcription, HIV life cycle”, “cell cycle”, and “cell cycle, apoptosis” (FDR p < 0.05; Supplementary Table S2). Full list of canonical pathway modules, categories, and terms may be found in Supplementary Table S3.
We also performed GSEA to identify the enriched biological processes in 3D spheroids compared to 2D monolayers (Fig. 3b). The top fifteen most significantly enriched biological process terms in EVTs cultured as 3D spheroids included categories: “immune processes”, “perception”, “chemotaxis”, “peptide cross linking”, “systemic processes”, and “response to zinc” (FDR p < 0.05; Supplementary Table S4). The top fifteen most significantly down-regulated biological process terms in 3D spheroids included categories: “protein complexes”, “metabolism, biosynthesis, catabolism”, “cell cycle”, and “transcription” (FDR p < 0.05; Supplementary Table S5). Full list of biological process modules, categories, and terms may be found in Supplementary Table S6.
Gene ontology analysis of biological processes
In parallel, we performed gene ontology (GO) analysis on the transcriptome microarray data to elucidate the over-represented biological processes based on differential expression (Fig. 4). The top fifteen most significantly up-regulated biological process terms in EVTs cultured as 3D spheroids compared to 2D monolayers included categories: “sensory perception”, “transport”, “response to stimulus, wound healing”, “G-protein signaling”, and “immune processes, angiogenesis, and response to stimulus” (FDR p < 0.05; Supplementary Table S7). The top fifteen most significantly down-regulated biological process terms in spheroids compared to monolayers included categories: “metabolic, biosynthetic, catabolic processes”, “transcription, translation, DNA replication”, and “cell cycle” (FDR p < 0.05; Supplementary Table S8). Full list of GO biological process categories, terms, and gene lists may be found in Supplementary Table S9.
Increased expression of markers related to epithelial-mesenchymal transition (EMT), cell-cell contact, angiogenesis, and invasion in EVTs cultured as 3D spheroids
We were particularly intrigued by the up-regulated biological process categories of “immune processes, angiogenesis, response to stimulus”, “response to stimulus and wound healing”, and “differentiation and vasculature development” in 3D spheroids, given the important role played by EVTs in placental invasion and vascular remodeling (FDR p < 0.05; Supplementary Table S9). We first investigated changes in markers in the EMT pathway via RT-qPCR, as EMT of trophoblasts closely underlies the initiation of placental invasion7,16. mRNA levels of upstream EMT markers WNT5A, TGFB1, and TGFB2 were significantly increased in 3D spheroids compared to 2D monolayers (p < 0.01 for all; Fig. 5a). Further downstream, integrin (ITG)A2, ITGA5, ITGB1, and vimentin (VIM) mRNA levels were increased in 3D spheroids compared to 2D, and occludin (OCLN) mRNA levels were decreased (p < 0.01 for all; Fig. 5b). Contrastingly, E-cadherin (CDH1) and zona occludens-1 (TJP1) mRNA levels were significantly increased in 3D spheroids (p < 0.01 for all; Fig. 5b), whereas they are typically repressed during EMT16. This may be explained in part by the fact that CDH1 and TJP1 were also markers of cell-cell contact and tight junction formation, which is promoted by 3D culture7. Claudin (CLDN)1 and CLDN4 mRNA levels, additional markers of tight junctions, were also significantly increased in 3D spheroids (p < 0.001 for all; Fig. 5b).
We further investigated placental angiogenesis and invasion gene markers shared across several of the biological process categories listed above. We found significant up-regulations in mRNA levels of HIF1A, VEGFA, VEGFC, and MMP9 in EVTs cultured as 3D spheroids compared to 2D monolayers (p < 0.01 for all genes; Fig. 5c). mRNA levels of TIMP2 remained unchanged (Fig. 5c). Protein levels of MMP9 were also increased in EVTs cultured as 3D spheroids, and protein levels of TIMP2 decreased (Fig. 5d; Whole blots provided in Supplementary Fig. S1). Altogether, this suggests that EVTs cultured as 3D spheroids exhibit enhanced expression of EMT activation, cell-cell contact, and tight junction formation in association with increased expression of angiogenesis and invasion.
EVT spheroids exhibit dynamic invasion when embedded into ECM
Considering that the spheroids expressed increased markers of invasion and angiogenesis, we next investigated their actual, functional invasiveness. We adapted and applied a novel, high-throughput spheroid invasion assay for use with our EVT spheroids17. Spheroids were embedded into ECM on day two, and observable invadopodia-like projections began to sprout from the spheroid core on day four, increasing in invaded area over the eight days (Fig. 6a). Mean spheroid invasion significantly increased over time, reaching approximately 60–70% invasion at day eight (p < 0.05; Fig. 6b). Using F-actin and DAPI immunofluorescent staining, the invadopodia-like projections were found to be actual cells moving away from the spheroid core and into the ECM (Fig. 6c). Control spheroids that were not embedded in ECM did not exhibit any invadopodia-like projections and remained as round spheres over the eight days (Fig. 6c). ECM-embedded, invasive spheroids further demonstrated significantly increased immunofluorescent staining for HIF1A protein (p < 0.05; Fig. 6d,f) and MMP9 protein (p < 0.05; Fig. 6e,g) compared to control spheroids.
Spheroid invasion impacted by exogenous drugs and compounds
Lastly, we were interested in testing the responsiveness of our 3D EVT spheroids to exogenous drugs and compounds. Spheroids were embedded into ECM and treated with lipopolysaccharide, dexamethasone, or THC every 48 hours for up to eight days. Lipopolysaccharide and dexamethasone have previously been shown to augment and inhibit placental invasion, respectively, thus would serve to validate the spheroids’ responsiveness18,19,20,21,22. Lipopolysaccharide (1–100 ng/mL and 10,000 ng/mL) indeed significantly augmented EVT invasion by day eight compared to the vehicle control (p < 0.05; Fig. 7a,b). Tube formation was observable for some LPS-treated spheroids, indicating the invasion of EVTs had extended up to the top of the ECM layer (Fig. 7b). Dexamethasone (100–10,000 nM) significantly inhibited EVT invasion by day eight compared to the vehicle control (p < 0.05; Fig. 7c,d). Given the dynamic invasive response to the validated compounds, we were interested in testing the effects of THC, a controversial, less-understood compound with early evidence to impact placental invasion23. THC (30 µM) significantly inhibited EVT invasion by day eight compared to the vehicle control (p < 0.05; Fig. 7e,f).
Three-dimensional spheroid and organoid cultures carry the potential to restore in vivo-like phenotypes in many in vitro organ systems7. Our study is the first to generate a specific 3D spheroid in vitro model of the trophoblast column that is capable of functional and responsive EVT invasion. We further demonstrate that 3D EVT spheroids: (1) express global transcriptomes that are distinct compared to 2D monolayers, (2) exhibit invasive and migratory gene and protein expression profiles alongside dynamic invasive behaviour when embedded into an ECM environment, and (3) may be studied by adapting the innovative spheroid invasion assay, which was originally pioneered by tumour researchers17,24, revealing responsiveness to exogenous drugs. Moreover, the simplicity of our ultra-low attachment microplate approach, continuous real-time monitoring capability, relative low cost of materials required, and use of a well-established cell line, collectively provides an accessible and versatile tool for researchers.
The major transcriptomic differences found in the GSEA and GO analyses altogether suggest that spheroid culturing conditions alone are sufficient to drive EVTs towards expressing a unique genetic profile compared to monolayers. Spheroids possessed enhanced expression profiles of EMT, cell-cell contact, tight junction formation, invasion, and angiogenesis, suggesting they may be a useful model to study EVT invasion. The initial detachment of EVTs from the trophoblast column and continual, but highly-measured, taxis into the maternal decidua is a key phenomenon observed in vivo that is not emulated in traditional invasion assays25. One major advantage of our model is the establishment of a solid spheroid core, which mimics the microarchitecture and physiochemical driving forces present at the trophoblast column of the anchoring chorionic villi in vivo (e.g., hypoxia/oxygen gradients, mechanical stimulation via ECM, etc.) Only EVTs that are truly invasive would be capable of detaching, invading, and migrating away from the core, whereas traditional assays use individualized cells that are primed to invade via serum-deprivation/reintroduction25. HIF1α, a protein known to be rapidly depleted by oxygen, was indeed augmented in our invading spheroids, suggesting the potential involvement of a hypoxia-driven mechanism26,27,28. HIF1α is a key regulator of invasion in vivo, and mice with Hif1α knocked out experience impaired placental invasion and angiogenesis compared to controls29. MMP9, a crucial matrix metalloproteinase in EVT invasion and known to be stimulated by hypoxia30, was also augmented at the protein level in invading spheroids. This reveals a preliminary mechanism that is consistent with in vivo EVT invasion, although more work must be done to further validate this.
Spheroid cultures also enable the co-existence of proliferative, quiescent and apoptotic states within the same population due to the nature of the 3D construct and the varying depths that cells reside from nutrient and oxygen sources, which better resembles the heterogeneous cellular organization present in vivo7,24. Accordingly, it has been well-demonstrated by cancer researchers that tumour spheroids exhibit a more physiological response and resistance in drug studies compared to monolayers24,31,32, and thus serve as more predictive in vitro models. Yang, et al.33 showed that pancreatic tumour cells cultured as spheroids exhibited greater invasive and migratory behaviour and more consistently formed tumours when injected into nude mice, compared to parental cells cultured as monolayers. Moreover, following paclitaxel chemotherapy treatment, ovarian cancer spheroids remained 20% more viable and proliferative than monolayers, which resembles a chemoresistance similarly seen in other tumours in vivo32. Following this hypothesis, our 3D EVT spheroids should also provide more a predictive response and resistance to drug exposure. For obvious ethical reasons, it was challenging to directly compare the drug responses of clinical populations given the lack of available data in pregnant women. We do, however, present novel findings that EVT spheroids are responsive to several exogenous compounds, with augmented invasion caused by lipopolysaccharide and inhibited invasion caused by dexamethasone and THC. It is well-documented that lipopolysaccharide increases cytokines like IL-634,35, which in turn stimulate MMP-2/9 activity to induce invasion18,19; whereas dexamethasone induces the opposite effect to inhibit invasion in vivo and in vitro20,21. While our results with lipopolysaccharide and dexamethasone are consistent with Librach, et al.22, there are some contrasting reports in literature that demonstrate an opposite or null effect34,35,36. This may be explained in part by the length of exposure, in which our model enables chronic exposures (eight days) compared to transwell invasion assays that must be acute (24–48 hours)34,35,36. However, this may also reveal a pitfall of transwell invasion assays as a whole, given that these studies do report a consistent downstream effect of the drug (e.g., increase and decrease in IL-6 via lipopolysaccharide and dexamethasone, respectively), but are simply unable to elicit the expected EVT invasive response34,35. Due to the volatility of the inflammatory response and longer-term drug exposures typically observed in clinical situations, it may be argued that our model’s chronic exposures with real-time measurements are necessary to accurately study the impact of drugs on invasion in vitro. Lastly, significant inhibition of EVT invasion by the highest dose of THC seen in our work is consistent with in vitro findings by Chang, et al.23, and may reveal a potential mechanism underlying the detrimental impact of this controversial, recreational and medicinal drug on placental and fetal development23,37.
While drug screening applications remain limited, our knowledge of stemness and differentiation in placental spheroids and organoids was recently advanced by some exciting studies9,11,12. Nandi, et al.9 demonstrated that HTR8/SVneo and primary placental cells cultured as spheroids may self-renew and differentiate down various trophoblast lineages (e.g., syncytiotrophoblast). Using Matrigel® drops and organoid media, Turco, et al.11 cultured primary cytotrophoblasts as organoids that shared high levels of transcriptomic and methylomic similarities with first trimester placental villi, and may also differentiate into invasive, HLA-G-expressing EVTs. Similarly, Haider, et al.12 used Matrigel® and organoid media to culture primary cytotrophoblasts as stem-like organoids with comparable gene expression profiles as primary cytotrophoblasts and the capacity to differentiate. Together with our current findings, there is accumulating evidence to support 3D culture as a useful and multifaceted model system for placental research.
In conclusion, we provide comprehensive rationale and evidence supporting the development of a microplate-based, spheroid invasion model for placental research. With enhanced invasive and angiogenic capability compared to 2D monolayer cultures and a dynamic response to exogenous compounds, we propose that our model has the potential to be further developed into a high-throughput, screening tool to preclinically assess the safety and impact of drugs and toxins on placental invasion. Given our current lack of knowledge surrounding the effects of both prescribed and illicit drug use in pregnant women, especially in Canada and the United States38, there is a great necessity to advance the development of alternative placental models.
Human placental EVT-derived HTR8/SVneo cells (generously provided by Dr. Peeyush Lala, Western University) were used due to the highly-conserved characteristics and gene expression profiles with placental EVTs39,40. HTR8/SVneo cells were cultured at 37 °C in a humidified atmosphere of 5% CO2/95% atmospheric air in RPMI-1640 (Corning), supplemented with 5% heat-inactivated FBS (Gibco), 1% (v/v) penicillin-streptomycin (Corning) and 1% (v/v) L-glutamine (Corning).
EVT spheroid generation
96-well ultra-low attachment (ULA) plates (Corning) were used to promote the self-assembly of HTR8/SVneo cells into three-dimensional (3D) cellular spheroids. HTR8/SVneo cells were seeded at 1,000 cells/mL, 5,000 cells/mL and 10,000 cells/mL into the ultra-low attachment plates. Media was replaced every 48 hours post-seeding by aspirating 100 µL of existing media, being careful to not disturb the spheroid, and dispensing 100 µL of fresh media into each well.
Live cell imaging and analysis of spheroid size
Live images of the spheroids were captured every 48 hours post-seeding at either 4X or 10X objective magnification using an Eclipse Ti-E Inverted Microscope (Nikon). Diameter (µm) of each spheroid was quantified using a manual measurement tool on ImageJ Fiji software (National Institute of Health). Average spheroid diameters were used to generate a linear regression and slope to track the growth over time (µm/day).
Cell viability staining assay
To confirm cell viability in spheroids over the experimental period, spheroids were incubated with calcein AM (Thermo Fisher; 1:100) for green fluorescence (live cells) and ethidium homodimer-1 (Thermo Fisher; 1:100) for red fluorescence (dead cells) for 30 minutes on days 4, 6 and 8. Spheroids were imaged in 5 µm z-stacks to capture all layers throughout the spheroid using an Eclipse Ti-E Inverted Microscope (Nikon), and all steps were compiled as a z-projection image. Images were analyzed using NIS Elements software (Nikon) to quantify the total areas covered by live or dead cells. The ratio of live to dead cells was then determined by dividing either live or dead cell area over the total area of the spheroid.
Hematoxylin and eosin (H&E) staining
Spheroids were fixed in 10% formalin within the ULA plates for 15 min at room temperature. An equal volume of liquified 2% agar was added to each well, mixed, and solidified on ice for 20 min. Solid agar pellets with spheroids were transferred into tissue cassettes, placed into 10% formalin for 48 hours, and stored in 70% ethanol. Agar pellets were then embedded in paraffin wax, sliced into 4 µm sections, stained with H&E, and imaged using an Eclipse Ti-E Inverted Microscope (Nikon).
Total RNA was extracted from cells using TRIzol Reagent (Invitrogen) and Direct-zol RNA MiniPrep Kit (Zymo Research), following the manufacturer’s protocol. RNA quality was assessed using the RNA 6000 Nano LabChip Bioanalyzer and TapeStation (Agilent), and 200 ng of RNA was processed to assess 20,000+ genes using the Clarion S Human microarray (Thermo Fisher) and GeneChip™ 3000 instrument system (Thermo Fisher), all carried out at the SickKids TCAG Microarray facility.
Normalization and output file generation was performed with Affymetrix Gene Expression Console (Thermo) using the Robust Multi-Chip Analysis (RMA) algorithm [RMA-Gene-full]41. Dendrogram was obtained by using all genes available on the platform excluding the control probes with the built-in hclust function in R. The height distance to convergence suggests the degree of similarity between the individual branches (samples). The similarity between the branches was measured based on the expression patterns exhibited by the genes in these samples.
Differential expression analysis was performed by using limma package in R42,43. Genes with FDR p-value < 0.05 were considered to be significantly regulated. Volcano plot was created using the limma package as well. Genes regulated with the absolute fold change ≥2 were used for further analyses. Examination and visualization of the significantly over-represented biological processes (Gene Ontology component) was performed by using the BINGO plugin in Cytoscape environment44. Gene Set Enrichment Analysis was performed by using C2, C5 and HALLMARK MSigDB collections on whole gene expression profiles45,46. Obtained results were further analyzed and visualized by using the EnrichmentMap plugin in Cytoscape environment47.
Real-time quantitative polymerase chain reaction (RT-qPCR)
Total RNA (500 ng) was further reserve-transcribed to cDNA as previously described8. Primer sets directed against gene targets of interest were designed through National Center for Biotechnology Information’s Primer-BLAST primer designing tool and synthesized at McMaster’s Mobix Labs (Table 1). Quantitative analysis of mRNA expression was performed via qPCR using SsoAdvanced™ Universal SYBR® Green Supermix (BioRad) and CFX384 Touch Real-Time PCR Detection System (BioRad). The cycling conditions were 95 °C for 10 min, followed by 40 cycles of 95 °C for 10 secs and 60 °C for 10 secs and 72 °C for 15 secs. Relative fold changes were calculated using the comparative cycle times (Ct) method, normalizing all values to the geometric mean of three endogenous control genes (18S, ACTB, GAPDH). The endogenous control gene was selected based on experimentally-determined Ct stability across all treatment groups. Given that all primer sets had equal priming efficiency, the ΔCt values for each primer set were calibrated to the average of all control Ct values, and the relative abundance of each primer set compared with calibrator was determined by the formula 2ΔΔCt, in which ΔΔCt was the normalized value.
Protein extraction and western blot
Total protein was extracted from cells using RIPA buffer supplemented with protease and phosphatase inhibitor cocktails (Roche). The solution was sonicated for 5 sec total, 1 sec per pulse, vortexed, and quantified by colorimetric DC protein assay (BioRad). Loading samples were prepared from fresh total protein extract, Laemelli Sample Buffer (4X) (BioRad) and β-mercaptoethanol, and heated at 90 °C for 5 min to denature the proteins. Proteins (10 μg/well) were separated by size via gel electrophoresis in Mini-PROTEAN® TGX Stain-Free™ 4–20% polyacrylamide gels (BioRad), and transferred onto polyvinylidene difluoride membranes using the Trans-Blot® Turbo™ Transfer System (BioRad). Membranes were blocked in 1x Tris-buffered saline-Tween 20 buffer with 5% non-fat milk, and then probed using anti-MMP9 (Abcam; ab76003; rabbit; 1:500) and anti-TIMP2 (GeneTex; GTX16392; rabbit; 1:500) antibodies diluted in the blocking solution. Donkey anti-rabbit (1:5,000) secondary antibody was used to detect the species-specific portion of the primary antibody, diluted in the blocking solution. Immuno-reactive bands were visualized using Clarity™ Western ECL Substrate (BioRad). Total protein was stained on the membrane using Amido Black and imaged to ensure even loading and transfer48.
EVT spheroid invasion assay
EVT spheroid invasion was studied by embedding the spheroids in an extracellular matrix (ECM) inside the ultra-low attachment plate. Growth-factor reduced Geltrex® (Thermo) was used as the ECM cocktail. On day 2, after the spheroids have self-assembled inside the ULA plate, 100 µL of media was aspirated from each well and replaced with 100 µL of Geltrex® (10 mg/mL) on ice30. The entire plate was then centrifuged at 300 g for 3 min at 4 °C to center the spheroids at a consistent position inside the well, and incubated for 60 minutes at 37 °C to allow the ECM to solidify. 100 µL of media was added on top of the solidified ECM. Brightfield images of the control and ECM-embedded spheroids were captured on days 2, 4, 6 and 8 at 4x or 10x objective magnification using an Eclipse Ti-E Inverted Microscope (Nikon) and analyzed using NIS Elements software (Nikon). Area of invasive edge of each spheroid was quantified at each time point by subtracting the average area of corresponding control spheroids from area of the invaded spheroid. Spheroid invasion percentage was then calculated as the ratio of invasive edge area to total invaded spheroid area and used as a relative measure to compare the invasiveness between spheroids17.
Spheroids were fixed overnight in 4% paraformaldehyde and permeabilized for 5 minutes with 0.1% Triton X-100 in PBS. Samples were then blocked for 1 hour using 0.01% Tween-20, 10% goat serum and 1% bovine serum albumin (BSA) in PBS. Afterwards, samples were incubated with HIF1 alpha primary antibody (Abcam; ab179483; rabbit monoclonal; 1:50) or MMP9 primary antibody (Abcam; ab76003; rabbit monoclonal; 1:250) overnight at 4 degrees, and then incubated with Goat Anti-Rabbit IgG H&L Alexa Fluor® 488 secondary antibody (Abcam; ab150077; goat polyclonal; 2 µg/mL) for 2 hours, as necessary. For F-actin staining, samples were incubated with CytoPainter Phalloidin-iFluor 555 reagent (Abcam; ab176756; 1:1000) for 2 hours. Samples were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; Santa Cruz; 1.5 µg/mL). All blocking and incubations were performed at room temperature, unless otherwise stated. Spheroids were visualized using an Eclipse Ti-E Inverted Confocal Microscope (Nikon). Z-stack images were taken in 10 µm steps to capture all layers and compiled as a z-projection image. Images were analyzed using NIS Elements software (Nikon).
Lipopolysaccharide (Escherichia coli serotype 055:B5; Sigma Aldrich) stock solutions were prepared from a dried powder at a concentration of 1 mg/mL in phosphate-buffered saline (Corning), and diluted to working concentrations of 1–10,000 ng/mL in RPMI-1640 media (Corning) at time of use. Dexamethasone (Sigma Aldrich) stock solutions were prepared from a dried powder at a concentration of 1 mg/mL in 90% ethanol, and diluted to working concentrations of 1–10,000 nM in RPMI-1640 media at time of use. Δ9 – Tetrahydrocannabinol (THC) stock solutions (Sigma Aldrich) were diluted to working concentrations of 1.875–30 µM in RPMI-1640 at time of use. Cells were treated with 100 µL of media containing either the vehicle control or drug treatment every 48 hours for up to eight days.
Prism 6 software (GraphPad) was used to statistically analyze all experimental results. Results were expressed as means of the normalized values ± standard error of the mean (SEM). Data sets were assessed for statistical significance (p < 0.05) through either an unpaired t-test, or one-way analysis of variance (ANOVA), and if significant, Tukey’s post hoc test to perform multiple comparisons.
All data generated or analyzed during this study are included in this published article (and its Supplementary Information files). Original dataset files are available in the Gene Expression Omnibus (GEO) repository [GEO accession number: GSE126844; https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc = GSE126844].
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We thank Dr. Hawke and Dr. Truant and their labs for providing access to the microscopes. We thank Dr. Hassell and his lab for guidance on spheroid fixation and histology. We thank NSERC for the Vanier Canada Graduate Scholarship (MW), and CIHR and NSERC for the Collaborative Health Research Program award (SR).
The authors declare no competing interests.
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Wong, M.K., Wahed, M., Shawky, S.A. et al. Transcriptomic and functional analyses of 3D placental extravillous trophoblast spheroids. Sci Rep 9, 12607 (2019) doi:10.1038/s41598-019-48816-8