Abstract
Cocaine use disorder (CUD), a major health crisis, has traditionally been considered a complication of the CNS; however, it is also closely associated with malnourishment and deteriorating gut health. In light of emerging studies on the potential role of gut microbiota in neurological disorders, we sought to understand the causal association between CUD and gut dysbiosis. Using a comprehensive approach, we confirmed that cocaine administration in mice resulted in alterations of the gut microbiota. Furthermore, cocaine-mediated gut dysbiosis was associated with upregulation of proinflammatory mediators including NF-κB and IL-1β. In vivo and in vitro analyses confirmed that cocaine altered gut-barrier composition of the tight junction proteins while also impairing epithelial permeability by potentially involving the MAPK/ERK1/2 signaling. Taken together, our findings unravel a causal link between CUD, gut-barrier dysfunction and dysbiosis and set a stage for future development of supplemental strategies for the management of CUD-associated gut complications.
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Introduction
Cocaine use disorder (CUD) has traditionally been considered a brain disorder in which alterations of the dopamine neurotransmitter system play a significant role in its psychoactive and addictive effects1. Recent studies have demonstrated that cocaine abuse is also related to changes in other neurotransmitters including serotonin, gamma-aminobutyric acid (GABA), norepinephrine, and glutamate2. Based on the premise that the limbic system of the brain comprising of a set of interconnected regions regulating pleasure and motivation, is the primary site of action of cocaine, helps explain its high potential for addiction and relapse1. Consequently, up to 22.5 million people worldwide are estimated to be affected by CUD, making it a major public health crisis with a high economic and social burden3.
In addition to its addictive properties, chronic cocaine use is also associated with weight loss, malnourishment, lack of appetite and reduced blood flow to the gastrointestinal tract4,5,6, suggesting thereby that cocaine also induces damage to the gastrointestinal (GI) tract. These studies suggest that cocaine could dysregulate the expression of tight junction proteins such as the claudin family of proteins, which are integral components of the intestinal barrier, thereby contributing to epithelial damage and increased permeability. Previous studies also have associated cocaine use with altered gut microbiota in humans with HIV infection7. Additionally, in another report, alterations in gut microbiota were shown to enhance sensitivity to cocaine reward and locomotor effects in rodents, thereby suggesting a link between gut microbiota and altered behavioral responses to cocaine8. Understanding how cocaine dysregulates the GI milieu is critical given the implications of gut microbiota in modulating addictive behaviors associated with cocaine abuse. In the current study, we sought to examine the mechanism(s) underlying cocaine-mediated alterations in gut homeostasis in mice administered cocaine.
Using the 16S rRNA sequencing of the distal colon fecal matter and excreted droppings of the mice administered cocaine, we confirmed significant alterations of gut microbial colonization, including the suppression of microbiota critical for butyrate synthesis, that is essential for the maintenance of normal gut homeostasis. Furthermore, our findings also identified ERK1/2-dependent gut-barrier dysregulation as a potential mechanism underlying cocaine-induced gut dysbiosis and the associated inflammatory environment. Overall, our findings demonstrate a critical association between CUD and gut-barrier alterations in regulating the gut microbial colonization and inflammatory milieu.
Results
Cocaine induces gut dysbiosis
To achieve detailed insight into the causal association of cocaine exposure and gut dysbiosis we first used 16S rRNA sequencing to analyze the microbiota from the fecal droppings and the colon of mice that were administered cocaine (20 mg/kg; i.p./day) for 7 consecutive days. We found 5061 OTUs that were common between the cocaine-exposed and control groups and, 9371 and 12420 OTUs that were specific to the saline controls and cocaine-exposed groups respectively (Fig. 1A). We further clustered the OTUs between the fecal droppings and the colon, and found 2362 OTUs that were common between the fecal droppings and colon irrespective of cocaine- or saline-exposure; and 11911 and 8926 OTUs that were specific for cocaine-exposed fecal droppings and colon, respectively (Fig. 1B). Species accumulation reached saturation (Fig. 1C), richness and evenness (Fig. 1D). Bacterial alpha diversity analyzed as observed species, or the Chao1 index remained unchanged between the cocaine-exposed and the saline administered mice (Fig. e,f). We next analyzed for shifts in beta diversity and observed alterations in bacterial composition based on the weighted UniFrac ANISOM diversity metric (R = 0.592, p = 0.001) thereby suggesting microbiota dissimilarities between cocaine- versus saline-administered mice. To better understand the effect of cocaine on the gut microbial composition, we next studied the effect of cocaine exposure on the taxonomic composition at the genus level. Cocaine exposure decreased colonization in the colon of four genera, i.e., Mucispirillum, Butyricicoccus, Pseudoflavonifractor and unclassified Ruminococcaceae with a concomitant increase in colonization of other genera such as Barnesiella, and unclassified members of Porphyromonadaceae, Bacteriodales, and Proteobacteria (Fig. 2A–C, Table 1). Fecal droppings of the cocaine-exposed mice also demonstrated decreased abundance of Lachnospiracea incertae sedis, Pseudoflavonifractor and Streptophyta and increased abundance of Turicibacter, Alistipes, Odoribacter and unclassified members of Porphyromonadaceae and Proteobacteria, (Fig. 1A–C, Table 2) thereby suggesting alterations of gut microbial homeostasis. Furthermore, it was also found that bacterial population in the colon and fecal droppings of cocaine-administered mice clustered separately from saline controls, based on the phylogenetic distance UPGMA weighted UniFrac (Fig. 1D) and principal coordinate analysis (Fig. 1E).
We next analyzed the effect of cocaine exposure at the species level and found depletion of unclassified species of Mucispirillum, Butyricoccus and Ruminococcaceae with a concomitant upregulation of unclassified species of Porphyromonadaceae, Bacterroidales, Barnisiella, Proteobacteria and Alistipes among others in the colon (Supplementary Table S1). Fecal droppings of cocaine-exposed mice demonstrated a similar dysregulation of microbial homeostasis involving downregulation of several bacterial species including unclassified Lachnospiracea incertae sedis, Streptophyta and uncultured Lactobacillus species (Supplementary Table 2). Cocaine-induced alterations in Lachnospiracea, Butyricoccus, Ruminococcaceae and other butyrate-producing bacteria suggested disturbances in gut energy balance as well as butyrate-mediated neural and immune functions.
Cocaine administration alters gut homeostasis
Next, we sought to examine whether cocaine exposure could affect gut homeostasis. We first assessed phosphorylation of ERK1/2 kinase that lies upstream of both CDX-2 and NF-κB - transcription factors that are critical for regulating gut homeostasis. While the levels of total ERK1/2 kinase remained unchanged in cocaine-administered mice, its phosphorylation was significantly upregulated in both the colon and ileum of cocaine-exposed mice compared with the control group (Fig. 3A,B). As shown in Fig. 3C,D, there was significant upregulation of CDX2 expression both in the colon and ileum of cocaine administered mice. A concomitant increase in the expression of both total and phosphorylated NF-κB was also observed in the colon of cocaine administered mice (Fig. 4A,B), thereby underscoring the role of cocaine in the modulation of gut homeostasis.
The next step was to determine whether cocaine exposure resulted in the induction of an inflammatory environment in the gut by analyzing the gene expression of specific chemokines and cytokines. Total RNA from the colon was subjected to qPCR analysis using a Qiagen mouse (common) cytokines and chemokines PCR array panel (PAMM 150, Qiagen). As shown in Fig. 4C,D, there was increased expression of several chemokines and cytokines and also downregulation of some others in the colon of cocaine administered mice compared to the control group. Further validation of these findings was also done using qPCR analysis for chemokines such as CCL-2, CCL-7, CXCL-10, CCL-11 as well as for cytokines IL-18 and IL-1β. As shown in Fig. 4E,F, cocaine exposure was found to induce a pro-inflammatory gut milieu. Notably, additional analysis using sera of either saline or cocaine-administered mice further demonstrated increases in known pro-inflammatory mediators such as Lipopolysaccharide Binding protein (LBP) and soluble CD14 ligand (sCD14) (Supplementary Fig. S1).
Cocaine administration alters mucosal epithelial barrier composition and integrity
Gut barrier dysfunction is often attributed to tight junction dysregulation, which includes the claudin family of proteins. Based on the premise that expression of claudins is altered in an inflammatory milieu, we next sought to assess for the barrier integrity by measuring the trans-epithelial electrical resistance (TEER) in the widely used in vitro model of intestinal epithelial cells - polarized monolayers of Caco-2 cells9,10,11. Confluent cell monolayers on transwell filter support (0.04 µM pore size) were either exposed to cocaine (10 µM) for 24 h or left unexposed followed by TEER measurements. As shown in Fig. 5A, cocaine exposure resulted in a significant reduction of TEER in Caco-2 cells. Complementary analysis of the paracellular permeability using apicobasal passes of a FITC-dextran dye (4 kDa) in these cells further demonstrated a significant increase in permeability compared with control cells (Fig. 5B).
We next assessed intestinal permeability in mice that were administered cocaine (20 mg/kg, i.p.) or saline (n = 9/group) for seven days and subsequently administered FITC Dextran (4 kDa) on the final day of cocaine injection followed by collection of blood after 4 h for detection of FITC by flourometry. As shown in Fig. 5c, cocaine increased intestinal permeability as FITC-dextran concentration in blood was higher in mice administered with cocaine compared with mice administered with the vehicle (p = 0.08).
We next examined the expression and cellular distribution of the important claudin proteins (claudin-1, 2, 3 and 7) in the intestinal epithelium. Interestingly, the expression of these tight junction proteins was found to be upregulated in the colon of cocaine administered versus saline control mice (Fig. 6A). Notably, the expression of the pore-forming claudin- 2 was upregulated the most with up to 4-fold increase compared with controls. Analysis using immunofluorescence staining and imaging further validated alterations in the expression and cellular distribution of these proteins (Fig. 6B).
Cocaine administration also modulated claudin expression in 3d-culture of colonic crypts potentially by inducing ERK1/2 signaling
We next sought to assess if alterations in the expression of claudin proteins can be recapitulated in 3d- culture of the colon crypt organoids that were either exposed to cocaine or left unexposed in the presence or absence of the ERK1/2 inhibitor U0126. As shown in Fig. 7A, exposure of colon crypt organoids to cocaine modulated expression of claudins and similar to the changes in vivo in cocaine-treated mice (versus control), and these changes in claudin expression were accompanied with a significant increase in expression of phosphorylated ERK1/2. There are reports, including ours, demonstrating MAPK/ERK-mediated regulation of claudin expression and localization12,13,14,15. We thus next sought to explore whether inhibition of ERK phosphorylation could restore cocaine-mediated disruption of claudin expression. To address this, colon crypt organoids were pretreated with the specific inhibitor of MAP/ERK1/2 kinase inhibitor - U0126 (10 µM), followed by exposure of the crypt cultures to cocaine and assessed for the expression of claudins. Pretreatment of crypt organoids with U0126 ameliorated cocaine-mediated expression of claudin especially claudin-2, compared to the control crypt organoids. In the same samples, the cocaine induced claudin-1, −3, & −7 expression was not affected by inhibiting ERK-activation (Fig. 7A,B). Taken together, our data suggested potential role of ERK1/2 signaling in cocaine induced modulation of specific claudin expression and barrier deregulation.
Discussion
Cocaine use disorder (CUD) is estimated to affect up to 22.5 million people worldwide3 resulting in marked changes in behavior and lifestyle emanating from its psychoactive and addictive effects. While most studies focus on the effects of cocaine on the brain, the current study was undertaken to assess the effects of cocaine on dysregulation of gut homeostasis including alterations in microbiota diversity, epithelial barrier function as well as innate immunity. Previous studies have demonstrated that depletion of gut microbiota by antibiotics in mice resulted in enhanced sensitivity to the reward and sensitizing properties of cocaine in the context of cocaine-mediated behavioral plasticity8. The current study provides insights into the mechanism(s) of cocaine-induced dysregulation of gut-barrier function, microbial colonization, and inflammation by directly examining the effect of cocaine administered by the intraperitoneal route on the intestinal microbial colonization, in both the fecal droppings and the colon. Most notably, our findings suggest that cocaine administration specifically depletes the Mucispirillum, Ruminococcaceae, Lachnospiracea, Pseudoflavonifractor and Butrycicoccus bacteria, that are key producers of short-chain fatty acids (SCFA) and related metabolites and that play critical roles in maintaining mucosal epithelial and immune homeostasis. Importantly, SCFA constitute a significant source of energy for the resident microbiota in the colon, and thus by inference depletion of SCFA producers could, in turn, contribute to dysbiosis16. In this regard, Kiraly et al. demonstrated that supplementation of SCFA reverses cocaine-mediated behavioral effects in antibiotic-treated mice, thereby underscoring the critical role of bacterial metabolites in modulating behavioral changes observed in these animals8. Since SCFA are well-known histone deacetylase inhibitors (HDACs)17, and also since previous reports have shown that administration of HDAC inhibitors in rodents resulted in reduced behavioral responses to cocaine18,19, it is plausible to envision that cocaine-mediated behavioral changes are regulated by a mechanism(s) involving alterations in gut microbiota. Intriguingly, similar to injection of cocaine, a recent study on rats chronically exposed to volatile cocaine also demonstrated similar alterations in the gut microbiota20.
Cocaine has been shown to activate NF-κB signaling pathway resulting in upregulated expression of pro-inflammatory cytokines and adhesion molecules in various cell types21,22,23,24,25,26. Furthermore, phosphorylated ERK1/2 that lies upstream of NF-κB is also known to activate CDX-2, a key transcription factor expressed in the gut and that modulates the expression of several genes involved in cellular proliferation, differentiation, and inflammation including integral proteins of the barrier27,28,29,30. The current study demonstrated that i.p. cocaine administration induced an inflammatory environment in the gut as evidenced by increased expression of multiple pro-inflammatory cytokines (IL-18, IL-1β) and chemokines (CCL-2, CCL-7, CXCL-10, CCL-11) and furthermore, this involved increased activation of the transcription factors NF-κB and CDX-2. Understanding the contribution of cocaine-induced gut inflammation in mediating neuroinflammation and, its role in the development of drug addiction warrants further investigation.
We also observed that cocaine upregulated the expression of claudin-2 in the colon. Claudin-2 has been shown to form paracellular channels that are permeable to cations and water31,32,33. Interestingly, upregulation of claudin-2 has also been shown to increase tight junction permeability to sodium ions and water during enteric pathogen infection34. Our data suggest that cocaine increased the expression of claudin-2, which in turn, contributed to increased epithelial permeability. Further studies in models of claudin genetic ablation are required to ascertain the contribution of claudins to cocaine induced barrier dysregulation and microbial translocation.
An intriguing potential mechanism that could provide a plausible explanation underlying cocaine-mediated dysregulation of gut homeostasis was the breach of the gut-barrier integrity, key player involved in regulation of intestinal inflammation. Recent studies have suggested a feedback mechanism between the gut-barrier function and inflammatory cytokines such as IL-13, TNF-α, IL-1β, and IFN-γ35,36,37,38,39, thereby implying a positive association between gut-barrier dysregulation, dysbiosis, and inflammation. Our in vitro and in vivo analyses support such a postulate and demonstrate that cocaine administration could impact the gut-barrier composition and integrity, leading in turn, induction of gut dysbiosis and inflammatory milieu. It is worth noting that deregulation of claudin protein expression and its altered cellular distribution has emerged as a critical factor in the disruption of mucosal barrier integrity31,34,40,41. Furthermore, the potential non-canonical role of these proteins in regulating mucosal epithelial and immune homeostasis including Notch-signaling have also been described42,43,44. Our data demonstrating that cocaine-induced alterations in claudin proteins were rescued by inhibition of ERK1/2 signaling further supports the possibility of a non-canonical role and warrants future in-depth investigation.
While our finding suggest that cocaine can disrupt both the microbiota and compromise gut barrier integrity, we cannot conclusively suggest whether cocaine impact on the microbiome leads to permeability changes or that cocaine effects on the gut epithelium lead to altered microbiota. Additional studies using germ-free or antibiotic-treated mice are warranted to help resolve this issue.
In summary, the overall findings herein demonstrate gut homeostasis dysregulation as a severe health concern related to abuse of cocaine and identify gut-barrier dysregulation, altered gut microbiota colonization, and inflammation as contributing factors, potentially acting in an inter-dependent manner. The possibility that these cocaine-mediated changes in gut homeostasis could subsequently contribute to neuroinflammation is highly plausible. Understanding cocaine-induced gastrointestinal tract dysregulation thus appears to be critical in light of the emerging role of the gut in modulating behavior especially the addictive behaviors.
Materials and Methods
Reagents
The following antibodies and reagents were purchased from the sources indicated: ERK 1/2 (R&D Cat# 9107S); pERK 1/2 (R&D Cat# 9101S); CDX2 (LSbio Cat# LS-B9317); NFĸB (Abcam cat # 16502), pNFĸB p65 (Cell Signaling Cat # 3031S); Claudin 1 (Invitrogen Cat# 51-9000); Claudin 2 (Invitrogen Cat# 32-5600); Claudin 3 (Invitrogen Cat# 341700); Claudin 7 (Invitrogen Cat# 374800); goat anti-rabbit-HRP (Santa Cruz Biotechnology Cat# sc-2004); goat anti-mouse-HRP (Santa Cruz Biotechnology Cat# sc-2005) and; actin (Sigma-Aldrich Cat# A1978). U0126 (Cat # 19-147) was from Sigma-Aldrich.
Mice and drug treatments
All animal procedures were performed in strict accordance with the protocols approved by the Institutional Animal Care and Use Committee of the University of Nebraska Medical Centre and the National Institutes of Health. Eight to ten weeks old male mice (C57BL/6N) were purchased from Charles River Laboratories (Wilmington, MA, USA). They were housed under conditions of constant temperature and humidity on a 12-h light, 12-h dark cycle, with lights on at 0700hrs. Food and water were available ad libitum. Mice were randomly divided into two groups administered either Cocaine (C5775, Sigma-Aldrich, 20 mg/kg, i.p.) or saline once a day for 7 days with 2 cages per group (n = 5/cage), The cocaine dose of 20 mg/kg, given once daily, has been shown to induce cocaine’s rewarding effects in C57BL/6 mice as reflected by the development of conditioned place preference in these mice45. Several studies from our group have utilized this dose over a 7-day period and observed significant molecular changes46,47,48. We thus used a cocaine dose (20 mg/kg; i.p./day) known to produce robust molecular and behavioral changes to examine the relationship between cocaine exposure and operational taxonomic units (OTU) in the gut. Mice were sacrificed by isoflurane anesthesia 1 hour post the final drug injection for gut removal. Droppings and colon fecal matter were collected for microbial DNA isolation. Mice droppings and fecal samples were immediately frozen on dry ice and then stored at −80 °C. Colon or ileum tissues were collected and used for extraction of protein and total RNA. mRNA and protein levels of pro-inflammatory cytokines and signaling proteins were assessed by quantitative RT-PCR and western blotting, respectively.
DNA isolation, 16S rRNA sequencing, and analysis
Bacterial DNA was extracted from fecal matter using the PowerSoil DNA isolation kit (MO Bio, Carlsbad, CA, USA Cat # 12888-100) according to manufacturer’s protocol. DNA samples were stored at −20 °C (or −80 °C for longer storage) until amplification. Polymerase chain reaction (PCR) of the variable 3 and 4 (V3 and V4) of the 16S rRNA gene was performed using 515F (5′-GTGYCAGCMGCCGCGGTAA-3′) and 806R (5′-GGACTACNVGGGTWTCTAAT-3′) primers49,50. Thermocycling conditions were 98 °C for 10 s, 58 °C for 30 s, 72 °C for 45 s for 35 cycles and 72 °C for 10 min. DNA was sequenced using the Illumina MiSeq platform at LC Sciences (Huston, Texas). Briefly, Operational Taxonomic Units (OTUs) were clustered at 97% sequence similarity using cd-hit. Taxonomy was assigned using the Ribosomal Database Project (RDP) classifier v.2.1 against the Greengenes reference database (13_8). Quantitative Insights Into Microbial Ecology (QIIME) was used for subsequent analysis of within- and between- community diversity (alpha and beta diversity). The relationship between 7-days cocaine administration (20 mg/kg) and microbiota was explored using Principal Coordinate Analysis (PCoA) on unweighted UniFrac phylogenetic distances between communities. The dissimilarity distance between the cocaine-exposed mice group and the saline control group was tested using ANOSIM statistical method.
RNA isolation and quantitative polymerase chain reaction (qPCR) from gut tissue
qPCR was performed as previously described51. Total RNA was extracted using Quick-RNA MiniPrep Plus (Zymo Research, R1058) as per the manufacturer’s protocol and quantified using NanoDrop 2000 spectrophotometer. RNA was transcribed into complementary DNA using Verso cDNA kit (Invitrogen, AB-1453/B) according to the manufacturer’s instructions. qPCR was performed by using TaqMan Universal PCR Mastermix (TaqMan, 4324018). The reactions were set up as follows: 10 µl TaqMan Mastermix, 1 µl forward/reverse primers and 7 µl molecular grade water and 2 µl cDNA. The QuantStudio 3 real-time PCR system (Applied Biosystems, Grand Island, NY) was used for program running. Mouse primers for CCL-2 (Mm00441242_m1, cat# 4331182); CCL-7 (Mm00443113_m1, cat# 4331182); CCL-11 (Mm00441238_m1, cat# 4331182); CXCL-10 (Mm00445235_m1, cat# 4331182); IL-1β (Mm00434228_m1, cat# 4331182), IL-18 (Mm00434226_m1, cat# 4331182) and actin (Mm99999915_g1, cat# 4331182) were purchased from ThermoFisher.
Serum collection and cytokine assays
Blood was collected via cardiac puncture and centrifuged (2000g) for 15 min. The serum was collected and stored at −80 °C. Serum sCD14 (R&D, MC140) and LBP (Abcam, ab213876) were quantified by ELISA according to the manufacturer’s instructions.
Western blotting
Colon tissues or crypts were lysed in RIPA buffer supplemented with a protease inhibitor cocktail (ThermoFisher Scientific, 78430) followed by ultrasonication for 15 sec, at 80% amplitude. Western blotting was performed as previously described51. Lysates were cleared by centrifugation at 12000 g (10 min; 4 °C). Using Thermo Scientific BCA kit (Cat# 23225), protein concentration was quantified by the BCA method. Equal amounts of protein (10–20 µg) were electrophoresed in a sodium dodecyl sulfate-polyacrylamide gel under reducing conditions. Following transfer, PVDF membranes (Millipore, IPVH00010) were blocked with 5% nonfat dry milk for 60 mins at room temperature. Next, membranes were probed with primary antibodies overnight at 4 °C, washed with TBS-T, then incubated with appropriate HRP-conjugated secondary antibodies and developed with SuperSignal West Dura or Femto substrate. Densitometric analyses were done using NIH ImageJ software (ImageJ v1.44, NIH). Protein amounts were normalized to β-actin.
Trans-epithelial electrical resistance (TEER) assay
Caco-2 cells were cultured on polycarbonate transwell filter supports (0.04 µM pore size), exposed to cocaine (10 µM) and trans-epithelial electrical resistance (TEER) was measured as previously described41,52. Caco-2 cells form cell monolayers with characteristics of mature enterocytes and have been widely used as an in vitro model of intestinal epithelial cells9,10,11,53,54. Results were expressed relative to the initial TEER value and presented as mean plus or minus standard error for three independent experiments.
FITC dextran permeability flux assay
Caco-2 cells were cultured on transwell filter support to confluency and exposed to cocaine 10 µM) for 24 h. Five microliters of FITC conjugated–dextran (120 mg/ml, 4 kDa, Cat # FD4-1G, Sigma) was added to the apical compartment of the transwell chamber system for 24 hours. The fluorescence intensity in the basal compartment was measured by fluorometry (excitation and emission wavelength of 485 nm and 520 nm, respectively).
Intestinal permeability
To access in vivo intestinal permeability FITC-dextran (120 mg/ml, 4 kDa, Cat # FD4-1G, Sigma) was orally gavaged into mice 4 hour prior to blood collection. After sacrifice, serum FITC-dextran fluorescence intensity was measured by Spectrofluorometry (Bio Tek Instruments).
Ex-vivo murine colon crypt organoid culture
Wild type mice (C57Bl6; 6–8 weeks) were sacrificed, colons were removed and flushed with cold PBS to remove the colon contents. Thereafter, the colon was opened longitudinally using a sharp scissor and epithelial layer was separated from the muscles using a razor blade and tweezer. Once separated, the epithelial layer was chopped into small pieces using a new razor blade and colonic crypts were cultured into a Matrigel bedding as for 3D-organoid culture. DMEM/F-12 culture medium supplemented with penicillin/streptomycin, glutamine, N2 and B27 was used to maintain the 3D-crypts cultures. For our studies, cocaine (25 µM) was mixed with the matrigel bedding prior to the culture of the colon crypts. MEK Inhibitor U0126 (10 µm) was used to study ERK1/2 activation upon cocaine treatment. Upon completion of the experiment, colonic crypts were removed from matrigel by using cold 1 mm EDTA in PBS and lysed in RIPA buffer for further immunoblot analysis.
Immunohistochemistry
Following removal of colonic contents, distal colonic sections were dissected and fixed in 10% formalin for 48 hrs. Sections were embedded in paraffin, sliced and stained with pNF-κB or Claudin-1, 2, 3 or 7 as previously described41.
Statistical analysis
Graphs and statistical analyses were performed using GraphPad software V6.0 (GraphPad Prism Software). Results between tests and controls were compared using the Student’s t test. P values less than 0.05 were considered statistically significant.
Data Availability
The data analyzed during the current study are available from the corresponding author on reasonable request.
References
Nestler, E. J. The neurobiology of cocaine addiction. Sci Pract Perspect 3, 4–10 (2005).
Shorter, D., Domingo, C. B. & Kosten, T. R. Emerging drugs for the treatment of cocaine use disorder: a review of neurobiological targets and pharmacotherapy. Expert Opin Emerg Drugs 20, 15–29, https://doi.org/10.1517/14728214.2015.985203 (2015).
Pomara, C. et al. Data available on the extent of cocaine use and dependence: biochemistry, pharmacologic effects and global burden of disease of cocaine abusers. Curr Med Chem 19, 5647–5657 (2012).
Cregler, L. L. & Mark, H. Medical complications of cocaine abuse. N Engl J Med 315, 1495–1500, https://doi.org/10.1056/NEJM198612043152327 (1986).
Nalbandian, H., Sheth, N., Dietrich, R. & Georgiou, J. Intestinal ischemia caused by cocaine ingestion: report of two cases. Surgery 97, 374–376 (1985).
Gibbons, T. E., Sayed, K. & Fuchs, G. J. Massive pan-gastrointestinal bleeding following cocaine use. World J Pediatr 5, 149–151, https://doi.org/10.1007/s12519-009-0030-5 (2009).
Volpe, G. E. et al. Associations of cocaine use and HIV infection with the intestinal microbiota, microbial translocation, and inflammation. J Stud Alcohol Drugs 75, 347–357 (2014).
Kiraly, D. D. et al. Alterations of the Host Microbiome Affect Behavioral Responses to Cocaine. Sci Rep 6, 35455, https://doi.org/10.1038/srep35455 (2016).
Valenzano, M. C. et al. Remodeling of Tight Junctions and Enhancement of Barrier Integrity of the CACO-2 Intestinal Epithelial Cell Layer by Micronutrients. PLoS One 10, e0133926, https://doi.org/10.1371/journal.pone.0133926 (2015).
Angelis, I. D. & Turco, L. Caco-2 cells as a model for intestinal absorption. Curr Protoc Toxicol Chapter 20(Unit20), 26, https://doi.org/10.1002/0471140856.tx2006s47 (2011).
Press, B. & Di Grandi, D. Permeability for intestinal absorption: Caco-2 assay and related issues. Curr Drug Metab 9, 893–900 (2008).
Steed, E. et al. MarvelD3 couples tight junctions to the MEKK1-JNK pathway to regulate cell behavior and survival. J Cell Biol 204, 821–838, https://doi.org/10.1083/jcb.201304115 (2014).
Chen, Y., Lu, Q., Schneeberger, E. E. & Goodenough, D. A. Restoration of tight junction structure and barrier function by down-regulation of the mitogen-activated protein kinase pathway in ras-transformed Madin-Darby canine kidney cells. Mol Biol Cell 11, 849–862, https://doi.org/10.1091/mbc.11.3.849 (2000).
Wang, Y., Zhang, J., Yi, X. J. & Yu, F. S. Activation of ERK1/2 MAP kinase pathway induces tight junction disruption in human corneal epithelial cells. Exp Eye Res 78, 125–136 (2004).
Ahmad, R., Kumar, B., Pan, K., Dhawan, P. & Singh, A. B. HDAC-4 regulates claudin-2 expression in EGFR-ERK1/2 dependent manner to regulate colonic epithelial cell differentiation. Oncotarget 8, 87718–87736, https://doi.org/10.18632/oncotarget.21190 (2017).
Rivera-Chavez, F. et al. Depletion of Butyrate-Producing Clostridia from the Gut Microbiota Drives an Aerobic Luminal Expansion of Salmonella. Cell Host Microbe 19, 443–454, https://doi.org/10.1016/j.chom.2016.03.004 (2016).
Koh, A., De Vadder, F., Kovatcheva-Datchary, P. & Backhed, F. From Dietary Fiber to Host Physiology: Short-Chain Fatty Acids as Key Bacterial Metabolites. Cell 165, 1332–1345, https://doi.org/10.1016/j.cell.2016.05.041 (2016).
Romieu, P. et al. Histone deacetylase inhibitors decrease cocaine but not sucrose self-administration in rats. J Neurosci 28, 9342–9348, https://doi.org/10.1523/JNEUROSCI.0379-08.2008 (2008).
Kennedy, P. J. et al. Class I HDAC inhibition blocks cocaine-induced plasticity by targeted changes in histone methylation. Nature neuroscience 16, 434–440, https://doi.org/10.1038/nn.3354 (2013).
Scorza, C., Piccini, C., Martinez Busi, M., Abin Carriquiry, J. A. & Zunino, P. Alterations in the Gut Microbiota of Rats Chronically Exposed to Volatilized Cocaine and Its Active Adulterants Caffeine and Phenacetin. Neurotox Res 35, 111–121, https://doi.org/10.1007/s12640-018-9936-9 (2019).
Hargrave, B. Y., Tiangco, D. A., Lattanzio, F. A. & Beebe, S. J. Cocaine, not morphine, causes the generation of reactive oxygen species and activation of NF-kappaB in transiently cotransfected heart cells. Cardiovasc Toxicol 3, 141–151 (2003).
Russo, S. J. et al. Nuclear factor kappa B signaling regulates neuronal morphology and cocaine reward. J Neurosci 29, 3529–3537, https://doi.org/10.1523/JNEUROSCI.6173-08.2009 (2009).
Yao, H., Allen, J. E., Zhu, X., Callen, S. & Buch, S. Cocaine and human immunodeficiency virus type 1 gp120 mediate neurotoxicity through overlapping signaling pathways. J Neurovirol 15, 164–175, https://doi.org/10.1080/13550280902755375 (2009).
Yao, H. et al. Molecular mechanisms involving sigma receptor-mediated induction of MCP-1: implication for increased monocyte transmigration. Blood 115, 4951–4962, https://doi.org/10.1182/blood-2010-01-266221 (2010).
Sahu, G. et al. Cocaine promotes both initiation and elongation phase of HIV-1 transcription by activating NF-kappaB and MSK1 and inducing selective epigenetic modifications at HIV-1 LTR. Virology 483, 185–202, https://doi.org/10.1016/j.virol.2015.03.036 (2015).
Yao, H. et al. Cocaine hijacks sigma1 receptor to initiate induction of activated leukocyte cell adhesion molecule: implication for increased monocyte adhesion and migration in the CNS. J Neurosci 31, 5942–5955, https://doi.org/10.1523/JNEUROSCI.5618-10.2011 (2011).
Coskun, M., Troelsen, J. T. & Nielsen, O. H. The role of CDX2 in intestinal homeostasis and inflammation. Biochim Biophys Acta 1812, 283–289, https://doi.org/10.1016/j.bbadis.2010.11.008 (2011).
Wang, D. & Dubois, R. N. The role of COX-2 in intestinal inflammation and colorectal cancer. Oncogene 29, 781–788, https://doi.org/10.1038/onc.2009.421 (2010).
Calon, A. et al. Different effects of the Cdx1 and Cdx2 homeobox genes in a murine model of intestinal inflammation. Gut 56, 1688–1695, https://doi.org/10.1136/gut.2007.125542 (2007).
Bhat, A. A. et al. Caudal homeobox protein Cdx-2 cooperates with Wnt pathway to regulate claudin-1 expression in colon cancer cells. PLoS One 7, e37174, https://doi.org/10.1371/journal.pone.0037174 (2012).
Weber, C. R. et al. Claudin-2-dependent paracellular channels are dynamically gated. Elife 4, e09906, https://doi.org/10.7554/eLife.09906 (2015).
Rosenthal, R. et al. Claudin-2, a component of the tight junction, forms a paracellular water channel. J Cell Sci 123, 1913–1921, https://doi.org/10.1242/jcs.060665 (2010).
Rosenthal, R. et al. Water channels and barriers formed by claudins. Ann N Y Acad Sci 1397, 100–109, https://doi.org/10.1111/nyas.13383 (2017).
Tsai, P. Y. et al. IL-22 Upregulates Epithelial Claudin-2 to Drive Diarrhea and Enteric Pathogen Clearance. Cell Host Microbe 21, 671–681 e674, https://doi.org/10.1016/j.chom.2017.05.009 (2017).
Luissint, A. C., Parkos, C. A. & Nusrat, A. Inflammation and the Intestinal Barrier: Leukocyte-Epithelial Cell Interactions, Cell Junction Remodeling, and Mucosal Repair. Gastroenterology 151, 616–632, https://doi.org/10.1053/j.gastro.2016.07.008 (2016).
Konig, J. et al. Human Intestinal Barrier Function in Health and Disease. Clin Transl Gastroenterol 7, e196, https://doi.org/10.1038/ctg.2016.54 (2016).
Schulzke, J. D. et al. Epithelial tight junctions in intestinal inflammation. Ann N Y Acad Sci 1165, 294–300, https://doi.org/10.1111/j.1749-6632.2009.04062.x (2009).
Pastorelli, L., De Salvo, C., Mercado, J. R., Vecchi, M. & Pizarro, T. T. Central role of the gut epithelial barrier in the pathogenesis of chronic intestinal inflammation: lessons learned from animal models and human genetics. Front Immunol 4, 280, https://doi.org/10.3389/fimmu.2013.00280 (2013).
Martini, E., Krug, S. M., Siegmund, B., Neurath, M. F. & Becker, C. Mend Your Fences: The Epithelial Barrier and its Relationship With Mucosal Immunity in Inflammatory Bowel Disease. Cell Mol Gastroenterol Hepatol 4, 33–46, https://doi.org/10.1016/j.jcmgh.2017.03.007 (2017).
Zeissig, S. et al. Changes in expression and distribution of claudin 2, 5 and 8 lead to discontinuous tight junctions and barrier dysfunction in active Crohn’s disease. Gut 56, 61–72, https://doi.org/10.1136/gut.2006.094375 (2007).
Ahmad, R. et al. Targeted colonic claudin-2 expression renders resistance to epithelial injury, induces immune suppression, and protects from colitis. Mucosal Immunol 7, 1340–1353, https://doi.org/10.1038/mi.2014.21 (2014).
Pope, J. L. et al. Claudin-1 regulates intestinal epithelial homeostasis through the modulation of Notch-signalling. Gut 63, 622–634, https://doi.org/10.1136/gutjnl-2012-304241 (2014).
Nakamura, T., Tsuchiya, K. & Watanabe, M. Crosstalk between Wnt and Notch signaling in intestinal epithelial cell fate decision. J Gastroenterol 42, 705–710, https://doi.org/10.1007/s00535-007-2087-z (2007).
Shinoda, M. et al. Early-stage blocking of Notch signaling inhibits the depletion of goblet cells in dextran sodium sulfate-induced colitis in mice. J Gastroenterol 45, 608–617, https://doi.org/10.1007/s00535-010-0210-z (2010).
Zhang, Y., Mantsch, J. R., Schlussman, S. D., Ho, A. & Kreek, M. J. Conditioned place preference after single doses or “binge” cocaine in C57BL/6J and 129/J mice. Pharmacol Biochem Behav 73, 655–662 (2002).
Liao, K. et al. Cocaine-mediated induction of microglial activation involves the ER stress-TLR2 axis. J Neuroinflammation 13, 33, https://doi.org/10.1186/s12974-016-0501-2 (2016).
Guo, M. L. et al. Cocaine-mediated microglial activation involves the ER stress-autophagy axis. Autophagy 11, 995–1009, https://doi.org/10.1080/15548627.2015.1052205 (2015).
Periyasamy, P., Guo, M. L. & Buch, S. Cocaine induces astrocytosis through ER stress-mediated activation of autophagy. Autophagy 12, 1310–1329, https://doi.org/10.1080/15548627.2016.1183844 (2016).
Parada, A. E., Needham, D. M. & Fuhrman, J. A. Every base matters: assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples. Environ Microbiol 18, 1403–1414, https://doi.org/10.1111/1462-2920.13023 (2016).
Caporaso, J. G. et al. Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms. ISME J 6, 1621–1624, https://doi.org/10.1038/ismej.2012.8 (2012).
Chivero, E. T. et al. HIV-1 Tat Primes and Activates Microglial NLRP3 Inflammasome-Mediated Neuroinflammation. J Neurosci 37, 3599–3609, https://doi.org/10.1523/JNEUROSCI.3045-16.2017 (2017).
Srinivasan, B. et al. TEER measurement techniques for in vitro barrier model systems. J Lab Autom 20, 107–126, https://doi.org/10.1177/2211068214561025 (2015).
Meunier, V., Bourrie, M., Berger, Y. & Fabre, G. The human intestinal epithelial cell line Caco-2; pharmacological and pharmacokinetic applications. Cell Biol Toxicol 11, 187–194 (1995).
Sambuy, Y. et al. The Caco-2 cell line as a model of the intestinal barrier: influence of cell and culture-related factors on Caco-2 cell functional characteristics. Cell Biol Toxicol 21, 1–26, https://doi.org/10.1007/s10565-005-0085-6 (2005).
Acknowledgements
This work was supported by NIH grant R01DA043138 (SB), the Nebraska Centre for Substance Abuse Research (NCSAR) and VA-Merit grant BX002761 (ABS).
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E.T.C., R.A., A.T., E.K., P.P., B.K. and D.F. performed the experiments and collected, analyzed and discussed the data. E.T.C., P.P., M.G., S.R., P.D., A.S. and S.B. designed experiments, discussed the data and drafted/revised the manuscript. S.B. conceived and drafted/revised the manuscript.
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Chivero, E.T., Ahmad, R., Thangaraj, A. et al. Cocaine Induces Inflammatory Gut Milieu by Compromising the Mucosal Barrier Integrity and Altering the Gut Microbiota Colonization. Sci Rep 9, 12187 (2019). https://doi.org/10.1038/s41598-019-48428-2
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DOI: https://doi.org/10.1038/s41598-019-48428-2
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