Abstract
In plants, low-dose of exogenous bacterial cyclic lipopeptides (CLPs) trigger transient membrane changes leading to activation of early and late defence responses. Here, a forward chemical genetics approach identifies colistin sulphate (CS) CLP as a novel plant defence inducer. CS uniquely triggers activation of the PATHOGENESIS-RELATED 1 (PR1) gene and resistance against Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) in Arabidopsis thaliana (Arabidopsis) independently of the PR1 classical inducer, salicylic acid (SA) and the key SA-signalling protein, NON-EXPRESSOR OF PR1 (NPR1). Low bioactive concentration of CS does not trigger activation of early defence markers such as reactive oxygen species (ROS) and mitogen activated protein kinase (MAPK). However, it strongly suppresses primary root length elongation. Structure activity relationship (SAR) assays and mode-of-action (MoA) studies show the acyl chain and activation of a ∼46 kDa p38-like kinase pathway to be crucial for CS’ bioactivity. Selective pharmacological inhibition of the active p38-like kinase pathway by SB203580 reverses CS’ effects on PR1 activation and root length suppression. Our results with CS as a chemical probe highlight the existence of a novel SA- and NPR1-independent branch of PR1 activation functioning via a membrane-sensitive p38-like kinase pathway.
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Introduction
In nature, plants are in a constant exposure to a plethora of harmful and beneficial microorganisms. Recognition of pathogenic molecular cues such as microbe-associated molecular patterns (MAMPs) or effector molecules results in MAMP-triggered immunity (MTI) or effector-triggered immunity (ETI), respectively1. These recognition events initiate a variety of early defence responses such as ROS production, MAPK activation as well as triggers downstream signalling mediated via phytohormones such as SA, jasmonic acid, and ethylene2. The small phenolic phytohormone SA plays a critical role in plant defence by inducing systemic acquired resistance (SAR)3. Pathogen attack triggers the expression of SA-biosynthetic gene ISOCHORISMATE SYNTHASE1 (ICS1)4 leading to an accumulation of SA which in turn is regulated by key regulators such as EDS15. SA is perceived by two proteins, NPR16 as well as the two NPR1 paralogs, NPR3 and NPR47. Increase in SA-levels monomerises NPR1 causing its translocation to the nucleus and subsequent expression of defence genes such as PR1.
An ability to modulate plant defence signalling processes in a spatio-temporal manner offers several advantages for crop protection8. Chemical genetics i.e. use of small bioactive chemicals as probes has now become an established approach to study plant biological responses. It has been applied to study a variety of phytohormones signalling networks, endomembrane trafficking as well as cell wall formation9,10. Chemical screenings have been instrumental in identifying SA-like defence inducing compounds11. Some well-characterised examples include: benzothiadiazole (BTH)12, 2,6-dichloro-isonicotinic acid (INA)13, 3,5-dichloroanthranilic acid (DCA)14, probenazole15, imprimatins16 etc., amongst others. All these chemicals induce canonical SA marker genes such as PR1 and require key SA-signalling components or SA itself for their bioactivity11.
In recent years, a number of CLPs such as surfactin, iturin, and fengycin etc., secreted by plant growth promoting rhizobacteria (PGPR), have been investigated for their defence-priming properties in plants. CLPs are natural products with wide structural diversity and strong antibiotic properties. They are synthesised by bacterial non-ribosomal peptide synthetases (NRPS) and share an overall similar architecture characterised by hydrophobic and acidic amino acids in their ring structure coupled to a fatty acid chain of variable length17. These compounds are now considered as a new class of MAMPs18,19,20,21 and are strong candidates for both biological pesticides and defence inducers22. Bioactive CLPs at low concentrations can sensitise plant lipid membranes leading to onset of early and late defence responses18,20,23. However, knowledge about associated signalling components and pathways is still in its infancy.
The polymyxin class of CLPs are strong antibiotics secreted by Paenibacillus polymyxa24. Typically, all polymyxins consist of a heptacyclopeptide fragment attached to a linear tripeptide with a fatty acid chain of varying length at the N-terminus and are polycationic via incorporation of the non-canonical diaminobutryic acid (Dab)25. Polymyxin E (PmE) or CS is an FDA approved commercial antibiotic used against Gram-negative bacteria infections26. At high concentrations CS has a strong biosurfactant property; however, at low concentrations it can modulate a variety of other functions such as inhibiting prokaryotic Hsp9027 and eukaryotic Hsp7028 chaperone activities or inducing innate immunity in nematodes by targeting a p38 kinase29.
The p38 kinase is a well-established membrane osmosensor30 known to play key roles during oxidative stresses in animals31. Plants lack classical p38 kinases32; however, several plant studies have implicated the activation of a p38-like kinase pathway during osmotic stress33,34, ABA-induced stomatal closure35, and cellular redox flux36. CS has been found to activate p38 kinase pathway in animal systems29,37, but its impact on plant p38-like kinase pathway is so far unknown.
In this study, we report on CS as a novel plant defence inducer. We identified CS through a phenotypic forward chemical genetic screen using PR1p::GUS defence reporter plants and a library of well-annotated commercial drugs of the Prestwick library. CS uniquely induces the SA-marker gene PR1 independently of both SA and NPR1 demonstrating its distinctness from many natural and synthetic plant defence inducers. Mode-of-action studies reveal CS does not require kinases involved in ROS or MAPK signalling rather, but works via an active 46 kDa p38-like kinase pathway.
Results
CS is a novel activator of the PR1 defence gene in Arabidopsis
The PR1 gene is a well-known defence marker strongly responsive to SA accumulation upon pathogen attack13. Accordingly, monitoring PR1 upregulation is an effective approach to identify potential plant defence activators11. Therefore, as a starting point to identify functional analogues of SA, we performed an in planta forward chemical genetic screen using the PCL (http://www.prestwickchemical.com/index.html) of 1280 small molecules (mostly approved drugs) along with 128 other handpicked compounds. We tested, in duplicates, 20 µM of each chemical on 14-day-old PR1p::GUS38 seedlings grown hydroponically in a 96-well plate format under long day conditions. Incubation with 200 µM SA or the solvent DMSO (1%) served as internal controls and bioactivity of compounds on PR1p::GUS induction was scored via an in situ GUS assay39. The resulting GUS-activity was converted to Z-scores as a criterion for hit-selection (Fig. 1A). For a confident primary-hit selection, we set a stringent threshold of Z-score ≥ 4 to focus on the strongest candidates with a bioactivity at least 4 times or higher than the DMSO control. In total, we identified six primary hits: acetyl salicylic acid (present twice in the chemical collection), 4-methyl umbelliferone, alexidine dihydrochloride, isradipine, and CS. About 80% of all tested compounds displayed a Z-score of around zero, indicating no effect on PR1 induction.
All six primary hits were retained in the secondary screenings. However, we decided to proceed further with CS as (i) CS and other polymyxins such as polymyxin B (PmB) and polymyxin B nonapeptide (PmBN)40 were commercially available (Fig. 1B), (ii) acetyl salicylic acid is a known SA-derivative41 and 4-methyl umbelliferone corresponded to the in situ GUS assay readout39, and therefore might well be an artefact (iii) CS displayed stronger bioactivity than alexidine dihydrochloride and isradipine, and (iv) unlike the other well-studied surfactin-type CLPs, CS polymyxins were never investigated in Arabidopsis before. To confirm the observed in planta effect of CS, we first performed a dose-response assay using 0.1 to 100 µM CS (Fig. 1C). PR1p::GUS expression steadily increased in a dose-dependent manner until 10 µM and declined sharply at ≥30 µM indicating the onset of toxic effects. The resulting half maximal effective concentration (EC50) of CS for PR1 induction was 5 µM. Next, we validated CS’ bioactivity, in vivo, by quantifying its effect on the accumulation of endogenous PR1 mRNA levels. Hydroponically grown 14-day-old Col-0 seedlings and soil-grown 28-day-old mature Col-0 plants were treated with 5 µM CS for 24 h. In both cases, CS strongly triggered a robust increase of PR1 transcripts compared to the DMSO controls (Fig. 1D). These results confirmed CS-mediated PR1 induction and also the robustness of our in situ GUS assays as a tool for chemical screenings. PR1p::GUS induction was detectable already after 30 min and steadily increased up to 24 h (Supplemental Fig. S1). Altogether, our results indicate that CS is a novel exogenous activator of the defence gene PR1 in Arabidopsis.
CS does not stress plants at its EC50 but affects seed germination and root growth
CS is a well-known biosurfactant courtesy of its cationic L-diamino-butyric acids (L-Dab) that interact with the anionic lipopolysaccharide molecules of Gram-negative bacteria to induce membrane disruption40. Plant membranes; however, have a different chemical composition than Gram-negative bacteria and hence certain CLPs such as surfactin are able to interact with plant membranes without inducing concomitant damage at low concentrations20,23. To test whether CS displays similar properties, we incubated Arabidopsis seedlings for 24 h with increasing concentrations of CS, followed by a subsequent Evans blue-based cell death quantification assay. In line with earlier reports for surfactin-type CLPs20,23, we observed cell toxicity only at ≥10 µM, indicating lower concentration of 5 µM are well-tolerated in two-week-old seedlings (Fig. 2A). Next, to understand the long-term effect of CS on plant growth and development, we germinated Col-0 seeds hydroponically in presence of 0 to 100 µM CS. A marked reduction in seed germination was noticed starting at 1 µM with complete inhibition at ≥3 µM (Fig. 2B). The seed germination assays showed a marked impact of CS on root emergence starting at concentrations of 1 µM. Upon further examination, we found that 0, 0.1, 0.5, and 3 µM CS impart a dose-dependent inhibitory effect on primary root growth elongation (Fig. 2C,D). Noticeably, a clear increase in root branching was observed upon application of CS (Fig. 2C), which strongly resemble the impact of its producer Paenibacillus polymyxa on its host root phenotype42. Furthermore, the impact of CS on root growth was independent of auxin signalling (Supplemental Fig. S2). Overall, our results indicate that CS triggers plant immunity along with an impact on plant root growth.
CS’ unique MoA induces PR1 independent of SA and SA-signalling components
Both natural and synthetic CLPs can trigger early or late defence responses such as ROS production and/or both SA and JA activation, respectively18,19,20,23,43,44,45,46. For compounds of the polymyxin class, in particular CS, we anticipated similar responses and, therefore, first tested its effect on three major phytohormonal signalling pathways: (i) JA-responsive VSP1p::GUS47, (ii) Auxin responsive DR5p::GUS48, and (iii) ABA responsive DC3p::GUS49. CS did not induce the reporter lines of JA, Auxin, and ABA, but SA-signalling thereby indicating its selectivity towards PR1 defence gene activation (Fig. 3A). Next, we tested effect of CS on ROS production, which is an excellent marker of stress induction upon CLPs application and is also known to trigger SA signalling. Col-0 seedlings treated with 5 µM CS showed no induction of ROS production while flg22, the classical ROS inducer, caused a clear ROS burst (Fig. 3B). Nevertheless, to eliminate the possibility of a late ROS induction contributing to CS’ bioactivity, we tested it on the ROS deficient rbohd/f mutant50. Again, CS caused a strong induction of PR1 in both wild-type as well as the rbohd/f mutant (Supplemental Fig. S3). Similarly, another defence regulator, the MAPKs (MPK6 and MPK3)51, were not induced by CS and no transient activation was noticed (Supplemental Fig. S4). Moreover, CS did not trigger sustained MAPK activation, a known pathway for SA-independent PR1 activation51, at later time points of 4, 8, and 24 h, while flg22 triggered a transient MAPK activation (Fig. 3C). Our results suggest that CS-induced signalling works via a unique pathway independent of ROS and MAPK components.
PR1 is a canonical marker gene of SA and its expression is severely impaired in SA-signalling mutants52. To investigate whether CS requires SA or its associated components in Arabidopsis, we tested its response in mutants of (i) SA biosynthesis: sid2-14, (ii) SA-accumulation: NahG plants52, (iii) SA signalling regulation: eds1-25, and (iv) SA signalling transduction: npr1-153. Surprisingly, application of 5 µM CS induced PR1 expression in all genotypes whereas SA as expected failed to induce PR1 expression in npr1-154 and NahG plants55 (Fig. 3D). These results, therefore, indicated a unique bioactivity of CS different from several well characterised PR1 inducers11. Similar, albeit a slightly varying, bioactivity was observed in 28-days-old plants of same genotypes (Supplemental Fig. S5). We next tested whether CS induces plants’ resistance to bacterial infection. We syringe-infiltrated leaves of 28-day-old wild-type as well as the SA-signalling and SA-deficient plants with or without 5 μM CS for 24 h, before injecting the leaves with a luminescent luxCDABE-tagged Pst DC3000 strain56 for rapid and reliable quantification of bacterial growth. In accordance with the results from CS-triggered PR1 gene expression induction (Fig. 3D), CS application enhanced resistance in all tested Arabidopsis mutant genotypes (Fig. 3E).
We next sought out to find whether CS works via the SA-independent signalling branch. We tested the well-characterised fmo1-1 mutant defective in the FLAVIN-DEPENDENT MONOOXYGENASE1 (FMO1) protein regulating SA-independent resistance57. Surprisingly, CS triggered strong PR1 expression in fmo1-1 plants (Fig. 3F) akin to wild-type. In summary, our findings indicate CS-triggered PR1 induction to be independent of both SA-dependent and SA-independent signalling factors and therefore occurs via an unconventional pathway.
Structure-activity-relationship assays with CS
To better understand the MoA of CS and its essential structural constituents, we tested some commercially available CS derivatives40. First, we tested PmB, an analogue with a d-phenyl alanine instead of d-leucine at ring position 6 of CS (Fig. 1A). Dose-response assays with PmB on PR1p::GUS displayed almost identical EC50 values of ca. 5 µM. Also, as observed with CS, PmB strongly reduced plant fitness at 30 µM (Fig. 4A) and failed to induce ROS production (Fig. 4B). We thereby concluded that the close structural homolog PmB displays CS like bioactivities. Next, we focused on the two structural ‘regions’ of polymyxins critical for their bioactivity: the fatty acid chain58 and the pentacationic polypeptide fragment59. We tested Polymyxin B nonapeptide (PmBN), an enzymatically derived PmB derivative lacking the fatty acid chain60 and surfactin a well characterised CLP with different amino acid composition from CS18,23,44 (Fig. 1B). While CS and PmB strongly induced PR1p::GUS expression, 5 µM of both PmBN and surfactin showed no agonistic effects (Fig. 4C). These results indicate that CS and PmB display a structure specific mode-of-action and the fatty acid chain is indispensable for its bioactivity.
CS functions via a stress-sensitive p38-like protein kinase pathway
The PCL is comprised of well-annotated compounds used already in different model systems61,62,63 but plants. We reasoned the knowledge gained from previous PCL-based screens, and specifically with CS, could therefore be useful to unravel the molecular mechanisms of CS in plants. Notably, from a recent chemical genetic study with PCL on living C. elegans, CS was identified as a defence inducer functioning via an active p38 kinase signalling pathway29. We thus hypothesized CS might have similar mode-of-action in plants since the p38-like kinase pathway is known to exist in Arabidopsis35,36,64, wheat root cells65, and sweet potato66 in addition to other species such as algae67.
We began with a highly selective mammalian p38-kinase pathway inhibitor, SB20358068, used frequently in plant studies35,36,65,66, to see whether it ablates CS’ bioactivity? 14-day-old PR1p::GUS seedlings were pre-treated with 3–100 µM SB203580 1 h before supplementing them with 5 µM CS for 24 h. Interestingly, application of SB203580 at ≥10 µM significantly diminished CS’ bioactivity (Fig. 5A). To avoid off-target effects at higher concentrations, we decided to use 10 µM SB203580 for all future experiments. To confirm the observed reversal of CS’ bioactivity by SB203580 is due to the interference of p38-like kinase pathway and not due to any off-target effect, we tested SB202474, an inactive SB203580 analogue having negligible effect on mammalian p38 kinase signalling69. SB202474 failed to suppress CS triggered PR1p::GUS activation indicating that the p38-like kinase pathway is indeed involved in PR1 activation by CS. Finally, to test whether SA also requires the p38-like kinase, we tested 10 µM of both SB203580 and SB202474 on SA treated PR1p::GUS seedlings. SB202474 showed no effect, but more remarkably SB203580 failed to reverse SA-triggered PR1 activation (Fig. 5B).
The p38 kinase in a well-known membrane sensor shown to be rapidly phosphorylated upon application of CS in C.elegans29 and mice37. We, therefore, asked whether CS phosphorylates (i.e. activate) the p38-like kinase pathway in Arabidopsis? Taking advantage from our previous SAR studies, we treated 14-day-old Col-0 seedlings with 5 µM of CS, PmB, PmBN, and surfactin for 24 h before detecting the phosphorylation status via Western blots. Only CS and its close structural and functional analogue PmB, induced the phosphorylation of a protein in ∼46 kDa detected by anti-phospho-p38 antibody (Fig. 5C). This protein band and antibody has been identified and used, respectively, in a number of reported studies36,65,66,67,70,71. Furthermore, in line with our previous findings in Fig. 4C, the functionally inactive CS derivative, PmBN, as well as the structurally divergent CLP, surfactin, did not trigger any phosphorylation (Fig. 5C). The inability of SB203580 to affect CS triggered phosphorylation was in accordance with previous reports demonstrating that SB203580 acts downstream of an activated p38 kinase pathway68. Collectively, our results present CS polymyxins as novel exogenous PR1 inducers in Arabidopsis working via an active p38-like kinase pathway.
Finally, application of SB203580 also reversed CS mediated primary root growth suppression (Fig. 5D), indicating it works via the p38-like kinase. Moreover, application of CS to tomato (Solanum lycopersicum cv. Moneymaker) leaf discs triggered significant PR1 upregulation, likewise Arabidopsis, signifying it may have useful application in other plants (Supplemental Fig. S6).
Discussion
Using our well established in planta forward chemical genetic screening on whole Arabidopsis PR1p::GUS reporter seedlings39, we identified two polymyxins, CS and PmB, as a novel class of exogenous plant defence modulators and strong inducers of the canonical SA-marker gene, PR1. Our mode-of-action experiments revealed that CS requires an active p38-like kinase signalling for its bioactivity. CS triggers the phosphorylation of a 46 kDa protein that shares strong biochemical and immunological similarity to the mammalian p38 kinase. Strikingly, CS-triggered PR1 expression was independent of SA as well as classical SA-dependent and SA-independent signalling components. This is unlike many of the established exogenous SA functional mimics11 and CLPs21.
CS belongs to the polymyxin family of CLPs. These compounds are biosynthesised by PGPRs found as beneficial root dwellers in host plants72. The plant root microbiome, including many CLP producing PGPRs, has recently been recognised as a novel modulator of plant innate immunity73. Functionally, CS is a potent biosurfactant used as an antibacterial drug26,74. Besides its well-known antibacterial properties, CS was recently identified as an exogenous modulator of animal innate immunity29. Nevertheless, the plant immune-modulatory properties of CS, and in general polymyxins, were unknown before our study. This is in a stark contrast to the rich body of literature on the immunomodulatory roles of other well-characterized CLPs such as surfactin in Arabidopsis20,21,44. Our findings demonstrate that despite both CLP families enhance plant immunity they nevertheless feature different mode-of-actions. In Arabidopsis, CS treatment resulted in a strong upregulation of endogenous PR1 mRNA levels in both seedlings and mature plants. Furthermore, upregulation of PR1 expression took place in a dose-dependent manner with strong toxic effects ensuing at 30 µM or higher. This was in accordance with the cell death assays which demonstrated CS-triggered toxicity at similar concentrations. Therefore, the cell death phenotypes at higher concentrations are most probably caused by the well-documented membrane disruption properties of CS. This effect was much pronounced in the seed germination and root growth assays where the plants were in constant exposure to CS for several days leading to the suppression of seed germination at ≥1 µM or primary root growth inhibition at ≥0.5 µM.
Plant membranes are key players for plant immunity as they mark the first line of defence and their integrity is therefore carefully monitored and a change in membrane fluidity can induce stress leading to defence induction46,75. Activation of ROS and MAPKs are some of the early responses upon perception of MAMPs and CLPs at plasma membrane. Surprisingly, CS did not require any of the signalling components involved in ROS and MAPK signalling many of which are located in the plasma membrane, making it a unique inducer of plant defence. However, it is clear CS trigger transient membrane changes to trigger downstream responses. This model is supported by our SAR studies which demonstrated PmB, a close functional analogue of CS, also generates strong PR1 induction. In contrast, PmBN, a CS analogue without the membrane interacting lipid chain moiety and surfactin, a CLP with different amino acid and fatty acid chain length, failed to replicate CS like responses. These results suggest that membrane perturbation trigger is a result of some specific ‘electrostatic’ and lipid chain membrane interaction mediated by CS and the non-toxic concentration of 5 µM CS is too low to induce a complete collapse of membrane integrity but may cause membrane sensitisation. The low bioactive concentration of 5 µM CS in Arabidopsis is significantly lower than the high 50–100 µM bioactive concentration range of surfactin76 and iturin A21 in Arabidopsis and other plant species.
A unique feature which sets CS apart from existing chemical and natural plant defence inducers is its ability to induce defence in SA-deficient plants as well as mutants of SA-dependent and SA-independent signalling components. Classical synthetic SA-mimics such as BTH77,78, Probenazole79, INA80, and N-cyanomethyl-2-chloroisonicotinamide (NCI)81 etc., require either or both SA and NPR1. CS, however, can induce PR1 expression in SA-deficient, sid2-1 and NahG, plants as well as in the mutant of SA-signalling npr1-1 and the mutant of SA-regulation eds1-2. CLPs such as iturin A of the surfactin family cannot upregulate PR1 in an npr1-5 mutant background21. Thus, our data suggest that CS displays a unique MoA. Furthermore, the ability of CS to trigger PR1 induction can be seen translated in the Pst DC3000 infection assays where CS enhanced plant defence in all the tested SA and SA-signalling mutants. Since CS is a known biosurfactant, it is fair to assume it will have toxic effects on Pst DC3000 upon direct contact. In our studies we applied CS 24 h before pathogen application and also observed a varying growth of the bacteria across different mutant genotypes. Although we cannot completely rule out that the observed enhanced plant resistance vs. Pst DC3000 after CS treatment stems from its known antibiotic properties, the variance of bacterial growth in accordance with the mutant genotype indicates that CS also acts via modulation of plant defence signalling pathways.
The requirement of an active p38-like kinase pathway by CS to induce PR1 expression is an intriguing discovery and is analogous to its bioactivity in C. elegans29. This also hints towards an evolutionary conserved role of this kinase in innate immunity. Even though, all known plant kinases lack the characteristic TGY (threonine-glycine-tyrosine) signature motif of animal p38 kinases32, many studies have strongly implicated the presence of a putative p38 homolog commonly called as “p38-like kinase”. Currently, no genetic mutants are available to test the significance of p38-like kinase in plant physiology. To overcome this bottleneck, plant biologists have created conditional knockdowns by using a highly selective mammalian p38 kinase pathway inhibitor, SB20358068, and have validated the outputs by using its inactive variant SB20247435,36. Similarly, scientists have routinely used the anti-phospho-p38-MAPK mammalian antibody to observe the activation of the p38-like kinase in plants such as sweet potato66, Arabidopsis35,36, perilla82, and wheat65 as well as in fungal species such as Dunaliella viridis83 or Dunaliella tertiolecta70, and algae84. The anti-phospho-p38-MAPK antibody does not cross-react with phoshpo-ERK proteins of plants65 underlying its specificity toward the p38-like kinase. We performed similar experiments using SB203580 and demonstrated that it severely hampers CS-triggered PR1 induction. Remarkably, SB203580 did not inhibit SA-triggered PR1 induction suggesting SA pathway works independent of p38-like kinase in Arabidopsis. Next, using the anti-phospho-p38-MAPK antibody, we showed that both CS and its analogue PmB can induce the phosphorylation of a 46 kDa protein kinase. Remarkably, surfactin, which is also a biosurfactant, and PmBN, an inactive CS derivative, could not trigger phosphorylation of the p38-like kinase. Another evidence of CS functioning via p38-like kinase was demonstrated by the root growth assay where SB203580 caused a significant reversion of CS induced root growth suppression. These results suggest a possible involvement of the p38-like kinase as a signalling juncture regulating growth and defence trade-off. However, further studies will be required to dissect this signalling branch. Nevertheless, our results suggest bioactivity of CS can well be a consequence of fine-tuned electrostatic interactions at the plant membrane or could also be transduced by a specific and yet unknown membrane-bound receptor leading to activation of the p38-like kinase pathway.
Overall, our results highlight an active p38-like kinase signalling pathway to be crucial for CS triggered PR1 activation and possibly root growth suppression. We show that polymyxins such as CS and PmB can trigger the activation of a 46 kDa protein kinase pathway sharing significant biochemical and immunological similarity to mammalian p38 kinases. In future, compounds targeting p38-like kinase activity may find application as novel plant defence modulators.
Materials and Methods
Plant material
Transgenic fmo1-157, rbohd/f50, eds1-25, npr1-153, sid2-14, NahG52, PR1p::GUS38, DC3p::GUS49, DR5p::GUS48, and VSP1p::GUS47 in Arabidopsis thaliana (Col-0) background were used for various bioassays.
Growth conditions
Surface-sterilised seeds were sown in 96-well plates (PerkinElmer Inc., Germany) containing half-MS basal salt (Sigma Aldrich) liquid medium supplemented with 0.5% sucrose. Seeds were stratified at 4 °C in the dark for 48 hours and later grown for 14 days under long day conditions (day/night cycle of 16/8 h at 21/19 °C). For soil grown Col-0, sid2-1, npr1-1, eds1-2, and NahG plants, seeds were sown directly on soil and stratified at 4 °C in the dark for 48 h. Plants were grown for about 28 days under long day conditions before performing biological assays.
Chemicals
The tested chemicals were taken from the Prestwick Chemical Library (1280 compounds, Illkirch-Graffenstaden, France) in addition to a collection of 128 handpicked compounds. CS was purchased from AK Scientific (San Francisco, USA), surfactin and PmB were obtained from Santa Cruz Biotechnology (Dallas, USA), PmBN and SB203580 from Sigma (Deisenhofen, Germany), and SB202474 from Cayman chemicals (Michigan, United States).
Activator screen using PR1p::GUS reporter
Screening was performed as described39. In brief, 14-day-old Arabidopsis seedlings harbouring the PR1p::GUS reporter were pre-treated with chemicals (dissolved in DMSO) at a final concentration of 20 µM for 24 h. Seedlings treated with 200 µM SA (dissolved in DMSO) or solvent DMSO (1%) served as internal controls. For each plant, at least two biological replicates were performed, and the resulting activity was normalised to control DMSO samples located on the same microplate.
in situ GUS activity quantification
The assay was performed as described previously39. In brief, 14-day-old seedlings grown in 96-well microplates were submerged in 150 µL lysis buffer (50 mM sodium phosphate, pH 7.0, 10 mM EDTA, 0.1% Triton X-100), containing 1 mM 4-MUG, at 37 °C for 90 min. At the end of the incubation period, 50 µL 1 M Na2CO3 (stop solution) was added to each well and the resulting 4-MU fluorescence was directly determined in a microplate reader as described before (excitation/emission wavelength of 365/455 nm). Activity was directly expressed as relative light units (RLU per seedling).
Root length quantification
Solid half-strength MS medium was supplemented with MES (0.5 g L−1), sucrose (5 g L−1), and 0.8% (w/v) Phytagel (Sigma Aldrich). Media was autoclaved and chemicals were added to media at 60 °C. Seeds were sown directly on the plates, stratified for 48 h, and grown for two-weeks under long day conditions. Images were taken and quantified using Image J software (https://imagej.nih.gov/ij/).
Oxidative burst assay
Luminol-based oxidative burst measurement was performed with 10-day-old Col-0 seedlings in 96-well white microplates. Seedlings were submerged in 100 μL H2O supplemented with 5 µM CS, 1% DMSO or 0.1 µM flg22. Luminescence detection was started immediately in a luminometer (Centro LB960, Berthold Technologies, Germany) by adding 100 µM luminol (L-012 from Wako Chemicals, USA) and 10 µg mL−1 horseradish peroxidase (Sigma-Aldrich, P6782) to the wells. Luminescence was measured every minute for the next 30 minutes. At least 5–10 biological replicates were used for each measurement.
Immunoblotting assays
Immunoblotting of MAPK as per51 and p38-like kinase as per36 was done with minor modifications. In brief, total protein was extracted from chemically-treated 14-day-old Arabidopsis Col-0 seedlings and 10 µg equal protein amounts were separated in 10% polyacrylamide gel by SDS-PAGE. For western blot, the gel was transferred to nitrocellulose membranes and probed with primary antibodies overnight at 4 °C. MAPK was detected using anti-phospho-p44/p42 MAPK antibodies (Cell Signalling Technology, 9102) while for p38-like kinase an anti-phospho p38-MAPK (Cell Signalling Technology, 9211) was used. Horseradish peroxidase-tagged goat anti-rabbit secondary antibodies were employed in both cases. Phosphorylation was detected using the Pierce ECL Western Blotting Substrate (Thermo Scientific, Rockford, USA) and imaged via ImageQuant LAS 4000 (GE Healthcare Life Sciences, Sweden).
Cell death assay using Evans blue dye
Cell death was examined by Evans blue staining as per85 with minor modifications. Briefly, 14-day-old Col-0 seedlings were vacuum-infiltrated in Evans blue (0.1% w/v) for 30 min and then maintained for at least 6 h in the vacuum chamber. To remove unbound dye post staining, extensive washing with autoclaved water was done. Dye bound to dead cells was solubilised in methanol (50% v/v) and SDS (1%) for 30 min on a shaker and quantified in a spectrophotometer at A600/A680.
Quantitative real-time PCR analysis
Total plant RNA was extracted using the manufacturer’s protocol (Bio-Budget technologies GmbH, Germany) and was used to make first strand cDNA using SuperScript™ II Reverse Transcriptase (Invitrogen). The cDNA was used as template in PCR reactions using gene specific primers (Sigma, Germany) and iQ™ SYBR® Green Supermix (Bio-Rad, Germany) on an iQ5 Real-Time PCR Detection System (Bio-Rad, Germany). The experiment was performed in three biological replicates (with one technical replicate for each biological replicate). The following primers (5′-3′) were used in our study:
Primers for arabidopsis
AtEXPR (reference gene) FP: GAGCTGAAGTGGCTTCCATGAC
AtEXPR RP: GGTCCGACATACCCATGATCC
AtPR1 FP: TCACAACCAGGCACGAGGAG
AtPR1 RP: CACCGCTACCCCAGGCTAAG
Primers for Tomato
SlHKG4 (reference gene) FP: GCTCCAGAAAGCTACATC
SlHKG4 RP: CGTCTCCTATAACGACTC
SlPR1 FP: CCGTGCAATTGTGGGTGTC
SlPR1 RP: GAGTTGCGCCAGACTACTTGAGT
Statistical analyses
Z-score analyses for the primary quantitative screening was done as per86. All statistical analyses done elsewhere were performed in Excel spreadsheets.
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Acknowledgements
We thank Brigitte Pickle for excellent technical assistance and Erich Kombrink for discussions. The work was funded by the University of Duisburg-Essen and a long term DAAD fellowship (A/10/74508).
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V.H. designed the study, performed all experiments, and analysed the data. M.N.S.S. performed the tomato experiment. F.K. helped with figures and text. V.H. and M.K. wrote the main manuscript text.
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Halder, V., Suliman, M.N.S., Kaschani, F. et al. Plant chemical genetics reveals colistin sulphate as a SA and NPR1-independent PR1 inducer functioning via a p38-like kinase pathway. Sci Rep 9, 11196 (2019). https://doi.org/10.1038/s41598-019-47526-5
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DOI: https://doi.org/10.1038/s41598-019-47526-5
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