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Sensitive Molybdenum Disulfide Based Field Effect Transistor Sensor for Real-time Monitoring of Hydrogen Peroxide


A reliable and highly sensitive hydrogen peroxide (H2O2) field effect transistor (FET) sensor is reported, which was constructed by using molybdenum disulfide (MoS2)/reduced graphene oxide (RGO). In this work, we prepared MoS2 nanosheets by a simple liquid ultrasonication exfoliation method. After the RGO-based FET device was fabricated, MoS2 was assembled onto the RGO surface for constructing MoS2/RGO FET sensor. The as-prepared FET sensor showed an ultrahigh sensitivity and fast response toward H2O2 in a real-time monitoring manner with a limit of detection down to 1 pM. In addition, the constructed sensor also exhibited a high specificity toward H2O2 in complex biological matrix. More importantly, this novel biosensor was capable of monitoring of H2O2 released from HeLa cells in real-time. So far, this is the first report of MoS2/RGO based FET sensor for electrical detection of signal molecules directly from cancer cells. Hence it is promising as a new platform for the clinical diagnosis of H2O2-related diseases.


Reactive oxygen species (ROS) play crucial roles in regulating DNA damage, signal transduction, cell proliferation and apotosis, etc.1,2,3,4. Hydrogen peroxide (H2O2), as a most common representative of ROS, is not only involved in several bodily disorders such as atherosclerosis, cancer, and Alzheimer’s disease, but also acts as an essential component in the physiological signaling pathways of healthy organisms, which is essential for cell growth, differentiation, migration, and immune system function5,6,7. Therefore, fast and accurate detection of H2O2 released from living cells is important for biological and clinical diagnostics application.

For detecting H2O2, there are many analytical methods, such as fluorometry8, spectrophotometry9, colorimetry10, electrochemical methods11,12, etc. Among these, the electrochemical methods for sensing H2O2 have been widely used due to their high sensitivity, fast response, and easy miniaturization. Most of electrochemical sensors involve functionalization of enzymes or proteins on the sensing interface13,14,15. Enzyme-based methods have been widely studied due to their remarkable sensitivity and specificity. However, the immobilization procedure of preparing the enzyme electrode has great influence on the biocatalytic activities of enzymes, leading to a limited stability and complicated immobilization procedure. Compared with enzymatic methods, the sensors based on nanomaterials (such as metal nanoparticles, carbon nanomaterials and metallic oxide nanostructures,) with high sensitivity and good stability brings H2O2 sensing to non-enzymatic era. Nanomaterials’ intrinsic catalytic characteristics (extremely small size and a large surface area per unit of volume) and their ability in scavenging reactive oxygen species in general can be used to mimic the catalytic activity of natural enzymes16,17. For example, graphene with large specific surface area, excellent electronic conductivity, and good chemical stability has been frequently reported to construct H2O2 sensing devices18. However, there are flaws in some ways including sensitivity, selectivity, and so on.

Recently, 2D sheet-like structure of transition metal dichalcogenides (TMDCs) has attained significant amount of interest due to their potential applications in nanoelectronics, sensing, and energy harvesting. Among these 2D TMDCs nanosheets, molybdenum disulfide (MoS2) is a naturally lamellar material with three atom layers (S-Mo-S) stacked together to form a sandwich structure. The unique feature renders thin MoS2 nanosheets considerable interest and application in catalysis, transistors, lithium ion batteries, and sensors, etc.19,20,21. Recently, MoS2 has been reported to directly detecting H2O2 without using enzyme and has shown intrinsic peroxidase-like activity. Lei et al.22 utilized excellent peroxidase-like activity of few layer MoS2 for the colorimetric detection of H2O2 with high sensitivity. Wang et al.23 fabricated a sensor for electrochemical detection of H2O2 released from cells based on MoS2 nanoparticles, and discovered the electrocatalytical activity of MoS2 nanoparticles toward the reduction of H2O2. Owing to the high sensitivity, rapid measurement, label-free detection, and compatibility with large-scale integrated circuit, field effect transistors (FETs) biosensors have attracted considerable interests24,25,26,27,28. Similarly, MoS2 based FET biosensor have also been applied for detecting biological molecules. Sarkar et al.29 has shown impressive sensitivity of the MoS2 based biosensor for detecting pH and proteins. Lee et al.30 and Jiang et al.31 utilized MoS2 as the sensing material for highly sensitive detection of DNA and mercury ion, respectively. Compared with zero band gap graphene, the advantage of MoS2 FET sensor is ascribed to the suitable band gap and high on/off ratio of MoS2. Although MoS2 can be used as the prospective candidate for the sensing channel material of FET and catalyst for the hydrogen evolution reaction (HER)32, so far little research is focused on utilization of MoS2 in the FET biosensor as a perfect catalyst toward H2O2.

In this work, we have prepared a high performance FET sensor with the introduction of MoS2 and reduced graphene oxide (RGO) for highly sensitive and specific detection of H2O2 from cancer cell, in which the MoS2 nanosheets is employed as the catalytic layer and RGO is used as the conductive layer. As illustrated in Fig. 1, a RGO sheet is drop-casted on the FET sensor surface between source and drain channel as a highly conductive bridge to facilitate rapid transport of electrons. Then the well-exfoliated MoS2 nanosheets are assembled on the surface of RGO. The MoS2 nanosheets act as an excellent catalyst and show highly catalytic activity toward H2O2. The as-prepared FET sensor responds fast and is extremely sensitive to H2O2 with the detection limit down to 1 pM. Furthermore, the MoS2/RGO FET biosensor is applied to monitor trace amount of H2O2 released from cancer cells.

Figure 1

Schematic diagram of the MoS2/RGO FET sensor for real-time monitoring of H2O2 release from cancer cell.

Results and Discussion

Characterization of MoS2 nanosheets and MoS2/RGO FET device

Ultrasonication has been proved to be a simple but an effective way to exfoliate graphite, bulk MoS2, and some other layered materials, because ultrasonic waves generate cavitation bubbles capable of breaking up the MoS2 crystalline and producing MoS2 nanosheets33. As known, N-methyl-2-pyrrolidone (NMP) is an excellent solvent for exfoliating 2D layered materials34. So we explored NMP as the solvent to prepare MoS2 nanosheets by utilization of ultrasonication in the experiment. The structure and morphology of the as-exfoliated MoS2 nanosheets were characterized by TEM. The TEM images in Fig. 2a clearly show that the well-exfoliated nanosheets were very thin, and a histogram of measured nanosheets size in Fig. 2b indicates that the average lateral sizes of MoS2 were 200–250 nm.

Figure 2

(a) Typical TEM images of the exfoliated MoS2 nanosheets. (b) Histogram of measured MoS2 nanosheet size. (c,d) XPS characterization of the MoS2 nanosheets. (e) SEM images of RGO sheet spanning across Au electrodes (f) SEM images of MoS2 nanosheets on the surface of RGO in the sensing channel.

Then, XRD was employed to characterize the MoS2 nanosheets. As shown in Fig. S1, the XRD spectra of bulk MoS2 crystals matched with the previously reported literature35. The typical diffraction peaks centered at 14.3° is attributed to the (002), which belongs to the bulk MoS2. After exfoliated, the characteristic peak disappeared, indicating the existence of lamellar form in the exfoliated MoS2. The results demonstrate the successful fabrication of MoS2 nanosheets.

As known, the MoS2 nanosheets contain stable hexagonal semiconducting phase (2H phase) and metastable metallic phase(1T). XPS was employed (Fig. 2c,d) to survey the spectrum of Mo and S and the surface chemical properties of the as-prepared MoS2 nanosheets. The peaks at 232.6, 229.4 and 226.5 eV, corresponded to Mo4+3d3/2, Mo4+ 3d5/2 and S 2s, respectively. In the S2p spectrum, S 2p1/2 and S 2p3/2 peaks also appeared at 163.5 eV and 162.3 eV, which is consistent with previously published papers35,36,37. These results show that the dominant 2H phase in the MoS2 nanosheets have been obtained from sonication-assisted exfoliation of MoS2 powder.

Moreover, the optical properties of MoS2 dispersion were investigated by UV-visible spectra. Fig. S2a shows the UV-visible spectrum of the diluted MoS2 dispersion in ethanol. As seen, the characteristic absorption bands appeared at approximately 400, 450, 610 and 670 nm. The two absorption peaks at about 610 and 670 nm were caused by A1 and B1 direct excitonic transition at the K point with energy separation. The peaks at 400 and 450 nm could be ascribed to the direct transition of M point from the deep valence band to the conduction band. All these results are in good agreement with the reported literatures38,39.

A fluorescence experiment was also conducted to verify that the prepared MoS2 was a structure of nanosheet. As is well known, the oligonucleotides of DNA can adsorb on the surface of the layered 2D TMDCs including MoS2, WS2, etc, via van der Waals interactions, and subsequently the layered 2D TMDCs could quench the fluorescence of single-stranded DNA due to fluorescence resonance energy transfer (FRET). However, pristine TMDCs powder can’t quench the fluorescence of single-stranded DNA40,41. Fig. S2b shows strong fluorescent emission at the wavelength of approximately 520 nm for the FAM fluorophore-labeled DNA (FAM-DNA). With the addition of the as-prepared MoS2, up to 97% quenching of the fluorescent emission was observed, showing that MoS2 could effectively quench the fluorescence of FAM-DNA. The result further indicates that the obtained MoS2 nanosheet is layered nanomaterial of high quality. Figure S3a represents typical Raman spectra of RGO, in which the D band at 1350 cm−1 and the G band at 1600 cm−1 were displayed, respectively. The Raman spectra results of MoS2 nanosheets show two characteristic peaks at 383 and 407 cm−1, respectively. The strong Raman peaks of the MoS2 nanosheets suggest that the exfoliated MoS2 nanosheets are of high quality.

The SEM image was employed to characterize the prepared MoS2 FET device. As shown in Fig. 2e, it was seen that a few layer RGO sheet was laid flat in the channel and was connected to a pair of Au electrodes. After MoS2 nanosheets were drop-casted on the surface of RGO, the dense MoS2 nanosheets were well-proportionally distributed on the sensing channel (Fig. 2f). All the SEM results demonstrate the successful fabrication of MoS2/RGO FET device.

Electrical properties of MoS2/RGO FET device

Current-voltage (I-V) curves were used to characterize the electrical properties of the MoS2/RGO-based FET device. As shown in Fig. 3, the transfer characteristic curves of the device were measured with RGO and MoS2/RGO FET devices, respectively. The ambipolar characteristics could be clearly observed at a small range of gate voltage (from −0.1 to 0.3 V) under ambient conditions. The dirac point of RGO transfer curve was found to be 0.1 V (Fig. 3a). After drop-casting MoS2 onto the surface of RGO, the Dirac point of the device shifted to left (0.08 V). When gate bias of Vg = 0.1 V was applied, the device showed n-type doping, indicating that the electronic conduction is dominant in graphene channels. This phenomenon is consistent with those reported by the literatures42,43,44. The Ids-Vds curve was obtained to further examine the electrical characteristics of the MoS2/RGO FET device in Fig. 3b. The drain-source current decreased with a slight reduction of the gate voltage, indicating that the device response was sensitive to the gate voltage.

Figure 3

(a) Transfer characteristic curves of the RGO-based FET device (black line) and MoS2/RGO FET device (red line). (b) The output curves of the MoS2/RGO FET device at different VGS value.

Real-time electrical detection of H2O2

The sensitivity of the MoS2/RGO FET sensor was investigated by applying the freshly prepared H2O2 solutions of increasing concentrations ranging from 1 pM to 100 nM to the sensor, and the real time measurements were recorded. The changes of ISD were used to monitor the responses of the MoS2/RGO FET sensor upon addition of H2O2 at various concentrations in real time. The sensor response to H2O2 was quantified using the normalized current changes (ΔI/I0 = (ISD–I0)/I0), where I0 is the initial current and ISD is the stabilized current after changing the concentration of H2O2. As shown in Fig. 4a, the ISD of FET sensor showed a gradual decrease as the concentration of H2O2 increased. The sensing mechanism may be attributed to generation of the positive charges upon addition of H2O2, leading to a conductance decrease of the MoS2/RGO FET sensor. As reported, MoS2 can play as peroxidase mimics22,45 for decomposing H2O2. For horseradish peroxidase (HRP), the reaction mechanism with H2O2 is to form the reactive enzyme intermediate compound and produce hydroxyl radicals and H+46. So the possible mechanism of MoS2 catalyzing H2O2 is similar to that of HRP. In such a case, a positive charge (H+) is generated when H2O2 is applied to the MoS2/RGO sensor. This is in good agreement with the literature, in which H2O2 can react with polypyrrole (PPy) to generate a positive charge on RGO/PPy nanotube FET-type sensor47. Furthermore, the applied gate bias of Vg = 0.1 V was less than the oxidation potential of H2O2 (>0.3 V), indicating that electrochemical oxidation of H2O2 did not take place on the sensor device, and the ISD did not come from the oxidation of H2O2. This means that the reaction of catalytic decomposition of H2O2 occurred on the surface of MoS2/RGO. As discussed above, since MoS2 is a sandwich structure composed of two sulfur atoms and one molybdenum atom, protons can be penetrated to the middle layer, and the improvement in catalytic performance is probably due to the activity enhancement of the active sites in MoS2 by the intercalated protons48. Because of its intrinsic structural characteristics, MoS2 can act as peroxidase mimics for decomposing H2O2, wherein it produces positive charges in the process of catalysis. The positive charges were then bound to the surface of RGO, thereby attracting their counterions in graphene and inducing n-type doping. As the concentration of H2O2 rose, more positive charges were generated and bound to the surface of the graphene, and the carrier concentration decreased correspondingly, leading to the decreased current. The mechanistic scheme is displayed as Fig. S4. Figure 4b shows the current change ratio (ΔI/I0) versus different concentrations of H2O2. The H2O2 concentration showed a linear response to the drain current change ratio. The linear relationship was described as |ΔI| /I0 = 0.46lgCH2O2 + 5.66 (the logarithmic value of H2O2 concentration defined as lgC). The as-prepared MoS2/RGO FET sensor was extremely sensitive to H2O2, and the limit of detection could be achieved down to 1 pM with the signal-to-noise ratio >3 (the noise level of the FET sensors was estimated by using PBS as baseline). The MoS2/RGO FET sensor shows the highest sensitivity compared with other H2O2 sensors, as shown in Table S1. The amazing sensitivity can be ascribed to both high conductivity of the RGO-based FET biosensor and high catalytic capability caused by MoS2 nanosheets. The positive charges generated in catalytic reaction could be sensitively detected by the MoS2/RGO FET, resulting in the conductance decrease of the FET sensor. Moreover, it was observed that the typical response time of this FET sensor to H2O2 was estimated to be less than 1 s, exhibiting that the FET sensor had a fast response. For comparison, the response of the RGO FET sensor (without MoS2) toward various concentrations of H2O2 (from 1 pM to 100 nM) was also investigated. The current change was negligible when different concentrations of H2O2 were added. An evident Isd decrease was seen till the concentration of H2O2 reached 100 nM (blue line, Fig. 4a). This further implies that MoS2 is able to decompose H2O2 effectively, thereby producing a positive charge. Furthermore, more charge carrier density is formed on the MoS2/RGO FET device than the RGO FET device, making the larger readable signal in current change at low H2O2 concentration range.

Figure 4

(a) Real-time detection of H2O2 with increasing concentrations in PBS buffer with the MoS2/RGO FET sensor (red line) and the RGO FET sensor (blue line). (b) The calibration curve of MoS2/RGO FET sensor to a series of H2O2 concentrations. Error bars represent standard deviations of measurements (n = 3). (c) Selectivity measurement with the addition of a series of interferents (PBS, 1 mM AA, 1 mM UA, 1 mM Glu, 1 mM GLY, 1 mM NE, 1 mM L-GA,) followed by 1 μM H2O2 solutions. (d) Histogram of the current change of the MoS2/RGO sensor to PBS, AA, UA, Glu, GLY, NE, L-GA and H2O2, respectively.

The specificity of the MoS2/RGO FET sensor towards H2O2 was further investigated by real-time recording ISD upon addition of a series of interfering species in 1 × PBS solution, including ascorbic acid (AA), uric acid (UA), glutamate (Glu), glycine (GLY), Noradrenaline hydrochloride (NE), L-glutamine (L-GA). As show in Fig. 4c, when 1 × PBS, 1 mM AA, 1 mM UA, 1 mM Glu, 1 mM GLY, 1 mM NE, 1 mM L-GA, respectively, were successively introduced to the MoS2/RGO FET sensor, negligible current change was observed. However, when 100 nM H2O2 was injected, a remarkable current response was observed, even in the case that interfering species of high concentration coexisted in the analyte. To directly demonstrate the response difference, current changes of the various substances were summarized (Fig. 4d). These results firmly exhibit high specificity of the MoS2/RGO FET sensor toward H2O2. Then, the repeatability and stability tests were also conducted to illustrate the excellent property of the MoS2/RGO FET sensors by using more than 3 sensors, respectively. Firstly, for stability test, the as-prepared MoS2/RGO FET device was stored in a vacuum oven for 3, 7, 10 and 14 days, respectively, then used for the detection of 100 nM H2O2. As described in the Fig. S5a, the shift of the dirac voltage was only changed 12.5% compared with its original value over 2 weeks. This signal decrease may be caused by the nonspecific surface adsorptions. Secondly, the repeatability of the MoS2/RGO FET sensors was also evaluated. One MoS2/RGO FET sensor was chosen to repeatedly detect 100 nM H2O2 concentration for 7 times, as shown in the Figure S5b. The dirac voltage change of the sensor remained nearly the same after 7 measurements and a relative standard deviation (RSD) was 2.1%. The results demonstrate high repeatability of the sensor.

Real-time monitoring of H2O2 released from HeLa cells

H2O2 plays a significant role in many cell functions and can be used as potential marker for tumor cells. Consequently, it is very meaningful to sensitively detect H2O2 from living cell because of its diverse biological functions. For this experiment, we first investigated the influence of weak acid environment on sensor’s performance from pH 6.4 to 7.4 (These different pH values were made by adding HCl or NaOH to PBS solutions, which were finally adjusted by a commercial pH meter). The results show that the weak acid environment did not have significant influence on the change of Dirac point after different pH values were applied (Figure S6). Then, real-time detection of extracellular H2O2 released from HeLa cells was performed by the MoS2/RGO FET sensor. As known, the HeLa cells can generate H2O2 when stimulated by phorbol 12-myristate 13-acetate (PMA, PMA is a potent activator of protein kinase C (PKC), which can activate PKC to produce H2O2). On the contrary, H2O2 can be decomposed by catalase. Before cell level measurement, HeLa cells were cultured for 24 h on the MoS2/RGO FET sensor surface by using a self-made plastic culture chamber. After cell culture, the cells were found to be in good condition, as seen in Fig. 5, Inset. Afterwards, the culture medium was replaced with the same amount of PBS solution. As shown in Fig. 5, when PMA (with the final concentration of 1 μg/mL) was added into the cell chamber, the current declined immediately and then slowly stabilized in a short time (red line). Based on the current change generated in Fig. 5, we could semi-quantify the released H2O2 from cells by the working curve in Fig. 3b. The H2O2 concentration was then calculated to be about 100 pM. On the contrary, when the catalase (300 U/mL, H2O2 scavenger) was mixed with the test solution, hardly any current change was obtained upon addition of PMA to HeLa cells (purple line). The catalase can metabolize H2O2 to water and oxygen, so hardly H2O2 could be monitored by the MoS2/RGO FET sensor. For control experiments, PMA was added to the MoS2/RGO surface without culturing the HeLa cells. On this occasion, barely any current change was obtained (blue line). The above-mentioned experiment results prove that the observed current response indeed came from H2O2 released from the HeLa cells. These results firmly demonstrate that the constructed MoS2/RGO FET sensor was capable of real-time monitoring H2O2 released from living cells.

Figure 5

Real-time current response of the MoS2/RGO FET sensors toward H2O2 after PMA was added into PBS buffer solution in the presence of HeLa cells (red line) and in the absence of HeLa cells (blue line).The purple line shows the case that H2O2 scavenger catalase was mixed with the PBS solution and added onto the devices containing HeLa cells. Inset: Optical microscope image shows the HeLa cells grown well on the MoS2/RGO FET sensor array.


In conclusion, we have constructed the MoS2/RGO FET sensor capable of monitoring H2O2 release from cancer cells in a highly sensitive manner. The MoS2 nanosheets were prepared by a simple liquid ultrasonication exfoliation method and the MoS2/RGO FET sensors were fabricated by drop-casting the MoS2 nanosheets to the RGO FET device. Compared to the previously published works regarding various sensors for H2O2 detection, our FET-type sensor showed a rapid response to the changes of H2O2 concentration and an ultrahigh sensitivity to 1 pM. In addition, the FET sensor also demonstrated high specificity toward H2O2 in the presence of AA, UA, Glu, GLY, NE, L-GA. Moreover, the device provided an enzyme-free detection platform for the real-time detection of H2O2 released from HeLa cells. This work opens a new way for constructing nonenzymatic FET sensors for detecting ROS released from cells, and helps understand the mechanism of H2O2 sensing by MoS2. From the perspective of sensor performance, the MoS2/RGO FET can be used as alternative methods for the detection of H2O2 in many fields, and may play a significant role in the clinical detection of H2O2-related diseases.



N-methyl-2-pyrrolidone (NMP), uric acid (UA), phorbol 12-myristate 13-acetate (PMA), glycine (GLY), ascorbic acid (AA), glutamate (Glu), Noradrenaline hydrochloride (NE), L-glutamine (L-GA) and catalase were purchased from Sigma-Aldrich (St. Louis, MO, USA). The pristine MoS2 powder and graphite flake powder used in the experiments were purchased from Nanjing XFNANO Materials Tech. Co. Ltd. (Nanjing, China). Ultrapure water was generated from Millipore water purification system (18.2 MΩ.cm resistivity, Milli-Q Direct 8). Hydrogen peroxide (H2O2, 30%), and other chemical reagents were purchased from Sinopharm Chemical Reagent Co. Ltd. (Beijing, China). PBS buffer solution used in this work was pH = 7.4.

Preparation of MoS2 nanosheets

The MoS2 nanosheets were prepared using a simple liquid ultrasonication method49,50,51,52. Briefly, 1 g of pristine MoS2 powder was added to 100 mL flask, after which 50 mL NMP was added to the flask as dispersion solvent. The mixture was sonicated (Power: 200 W, Frequency: 20 KHZ) until to obtain a black homogeneous suspension at room temperature (usually needs 10 h). After that, the resultant dispersions were centrifuged for 30 min at 2000 rpm and then the top 2/3th part of the supernatant were decanted. To remove NMP and determine the concentration of the MoS2 nanosheets in the dispersion solvent, the dispersion was vacuum filtered through a nylon membrane with a pore size of 0.22 μm, followed by washing the membrane with large amount of distilled water and ethanol. The resultant film was dried for 24 h at 60 °C in vacuum oven. The MoS2 nanosheets powder was then peeled from the resultant film.

Fabrication of the MoS2/RGO sensor

The RGO-based FET device was produced by the previously reported method25. Firstly, 10 mg of GO was added to 10 mL of 98% hydrazine followed by sonication for 45 min to produce a black suspension of hydrazinium graphene, and the suspension was placed for 1 week to obtain the thorough reduction of GO. The resulting RGO suspensions could be stable for months with little aggregation. To construct the RGO layer on the pre-fabricated FET chip, diluted RGO suspension (0.15 mg/ml) was drop-casted onto the channel and thermally annealed at 80 °C for 2 h in order to enhance the contact between the RGO and the electrodes. In order to prepare the MoS2/RGO FET sensor, the MoS2 nanosheets powder was dispersed into ethanol to form MoS2 nanosheets dispersion with the concentration of 1 mg/mL. Then MoS2 dispersion was drop-casted on the surface of RGO. The whole device was thermally annealed at 80 °C for 0.5 h in vacuum oven for enhancing the contact among the MoS2, RGO and electrodes.

Electrical detection of H2O2 in PBS solution

Electrical detection of H2O2 was monitored in a liquid gate environment in real time under a constant bias voltage of 10 mV and liquid gate of 0.1 V. As described in our previously published papers25,26,27,28, a silver wire was used as the liquid gate in this work. When the measurement was performed, the silver wire was immersed in buffer solution. The different concentrations of H2O2 were determined by diluting H2O2 (30%) using 1 × PBS buffer solution. H2O2 was manually added to the detection chamber with a gradually increasing concentration ranging from 1 pM to 100 nM for the sensitivity experiment. The specificity experiment was conducted using the same method for discriminating different interferents.

Cell Culture

HeLa cells were purchased from cell bank of Xiangya Medical College (Changsha, China). They were routinely cultured in Dulbecco’s Modified Eagle Medium cell culture medium containing 10% fetal bovine serum (FBS) in a culture flask and supplemented with 1% penicillin at 37 °C, 5% CO2. HeLa cells were digested by trypsin from culture flask after growing to 90% confluence.

Electrical detection of H2O2 released from HeLa cells

For real-time detection of H2O2, a self-made liquid reservoir was mountained on the sensing channel (Fig. S7). After that, the total device was sterilized for 30 min via ultraviolet in a biosafety cabinet. Then the sensor was used for cell culture experiments. HeLa cells were seeded on the MoS2/RGO sensors confined in a self-made liquid reservoir at a density of ~1 × 104 cell/cm2. After 10 h of incubation, cells were used for stimulation and detection. Upon detection, the culture medium was then changed by the 1 × PBS. After reaching a steady-state baseline, PMA (1 μg/mL) as the H2O2 stimulant was introduced into the self-made liquid reservoir and H2O2 released from HeLa cells was detected by the real-time working mode present in the form of changes in current. Then catalase (300 U/mL) was injected into the liquid reservoir for the purpose of degrading H2O2, as the control experiment. The electrical measurement condition was the same as above described.


The morphology of the as-prepared MoS2 was characterized by TEM (JEOL JEM-2100, Japan) operated at 200 kV. The MoS2 dispersion was further diluted with ethanol and dropped on a carbon-coated film copper grid for subsequent TEM observation. The X-ray photoelectron spectroscopy (XPS) analysis was conducted using an ESCALAB 250 Xi XPS system (Thermo Fisher Scientific, American). UV-visible spectra were measured by UV-2550 (Shimadzu Co. Ltd., Japan). Raman spectra were taken by using Invia Renishaw spectrometer (RM 1000, England) equipped with 514.5 nm laser line. X-ray diffraction (XRD) analysis was conducted on PANalytical X’Pert Pro diffractometer (PANalytical, Holland). The fluorescence spectra were obtained by a Hitachi F-4600 spectrophotometer (Hitachi Co. Ltd., Japan). Scanning electron microscopy (SEM) images were obtained on a field-emission scanning electron microscope (Zeiss, Germany). All electrical measurements were recorded with a Keithley 4200 semiconductor characterization system and a shield probe station (Everbeing BD-6, Taiwan).


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We gratefully acknowledge financial support from the National Natural Science Foundation of China (NOs. 21475034, 21505037 and 21675041).

Author information

C.Z., Y.L. and G.J.Z. designed and conducted the study, Y.L. and G.J.Z. supervised the project, M.X. and Z.Z. participated in the experiments of preparation of the FET chips. C.Z., X.J., J.M. and Y.S. participated in the experiments of preparation of the MoS2, cell culture and real-time detection. C.Z., Y.L., H.Z. and G.J.Z. wrote the manuscript. All authors reviewed the manuscript.

Correspondence to Yutao Li or Zhiyong Zhang or Guo-Jun Zhang.

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Sensitive Molybdenum Disulfide Based Field Effect Transistor Sensor for Real-time Monitoring of Hydrogen Peroxide

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