Abstract
Congenital muscular dystrophy with laminin α2 chain-deficiency (LAMA2-CMD) is a severe muscle disorder with complex underlying pathogenesis. We have previously employed profiling techniques to elucidate molecular patterns and demonstrated significant metabolic impairment in skeletal muscle from LAMA2-CMD patients and mouse models. Thus, we hypothesize that skeletal muscle metabolism may be a promising pharmacological target to improve muscle function in LAMA2-CMD. Here, we have investigated whether the multifunctional medication metformin could be used to reduce disease in the dy2J/dy2J mouse model of LAMA2-CMD. First, we show gender disparity for several pathological hallmarks of LAMA2-CMD. Second, we demonstrate that metformin treatment significantly increases weight gain and energy efficiency, enhances muscle function and improves skeletal muscle histology in female dy2J/dy2J mice (and to a lesser extent in dy2J/dy2J males). Thus, our current data suggest that metformin may be a potential future supportive treatment that improves many of the pathological characteristics of LAMA2-CMD.
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Introduction
Mutations in the LAMA2 gene encoding laminin α2 chain cause congenital muscular dystrophy with laminin α2 chain-deficiency (LAMA2-CMD), a very severe muscle disorder. Under normal conditions, laminin α2 chain forms the heterotrimeric protein laminin-211 (together with laminin β1 and γ1 chains) and this extracellular matrix protein is highly expressed in the basement membranes of muscle and Schwann cells. Laminin α2 chain is either completely or partially absent in LAMA2-CMD and the clinical manifestations include profound hypotonia at birth, widespread muscle weakness, proximal joint contractures, inability to stand and walk, scoliosis, dysmyelinating neuropathy and white matter abnormalities1. At the histological level the skeletal muscle pathology comprises muscle fiber size variation, the presence of regenerating and necrotic fibers, vast inflammation and extensive proliferation of connective tissue2.
In order to obtain novel insights into the molecular mechanisms underlying LAMA2-CMD, we previously performed transcriptional and proteomic profiling of affected skeletal muscles from LAMA2-CMD mice. A majority of the differentially expressed genes and proteins were found to be involved in various metabolic processes3,4. Subsequently, we demonstrated functional bioenergetic impairment with reduced mitochondrial respiration and a compensatory upregulation of glycolysis in human LAMA2-CMD muscle cells5. Thus, from these studies, we concluded that skeletal muscle metabolism may be a promising pharmacological target to improve muscle function in LAMA2-CMD patients.
Metformin, a biguanide derived from Galega officinalis, has been used for more than 50 years to treat type II diabetes6. Despite long clinical use, its mechanisms of action still remain obscure, but several studies have demonstrated the effectiveness of metformin in skeletal muscle. For example, it was recently demonstrated that six weeks of metformin treatment increased expression of non-metabolic and metabolic-related genes in adipose and muscle tissue in old humans7. Furthermore, positive non-metabolic effects in exercised mdx mice (a mouse model of Duchenne muscular dystrophy) were observed after 20 weeks of metformin treatment, with improved skeletal muscle histopathology and force8. Moreover, metformin has been shown to protect skeletal muscle from cardiotoxin-induced degeneration9, increase physical performance in sedentary mice10 and enhance Pgc1α in dystrophin-deficient mdx muscle11. Also, an open-label proof-of-concept study demonstrated improved muscle function in four out of five ambulatory Duchenne muscular dystrophy patients treated with L-arginine and metformin12. Therefore, we reasoned that metformin might improve muscle function and delay disease progression in LAMA2-CMD. Hence, in this study, we have treated dy2J/dy2J mice with metformin. Dy2J/dy2J mice exhibit a mutation in the N-terminal domain of laminin α2 chain causing a laminin polymerization defect13. Consequently, dy2J/dy2J mice present a relatively mild muscular dystrophy with the first symptoms appearing at around 3–4 weeks of age and dy2J/dy2J mice typically live more than 6 months2,13,14. We demonstrate that dy2J/dy2J mice treated with metformin display improved muscle structure and function. Importantly, we also analyzed the gender factor in the progression of the disease and demonstrate sex differences.
Results
Weight gain differences in male and female dy 2J /dy 2J mice
To analyze the gender factor in the progression of the disease, males and females were separated in wild-type (WT) and dy2J/dy2J groups. As expected, the initial body weight was significantly different between males and females in both WT and dy2J/dy2J groups (Fig. 1A). The same trend was observed for the final body weight, with gender and disease significantly different when analyzed by two-way ANOVA (Fig. 1B). Notably, male dy2J/dy2J mice displayed similar weights as female WT mice (Fig. 1A,B). When weight gain was compared between dy2J/dy2J males and females, we noted that dy2J/dy2J males gain around 85% more weight than dy2J/dy2J females (Fig. 1C). Bearing this data in mind, metformin treatment was performed in males and females separately.
Metformin treatment increases water intake and weight gain in female dy 2J /dy 2J mice
We treated mice with metformin (250 mg/kg, daily oral gavage) during four weeks. The dose was chosen based on a small pilot study in which metformin at 100 mg/kg (daily oral gavage for four weeks) enhanced grip strength in female dy2J/dy2J mice but the increase was not statistically different from untreated dy2J/dy2J mice (data not shown).
We first assessed the effects of metformin administration on water intake, food intake and weight gain. In dy2J/dy2J females, we observed a 34% reduction of water intake compared with WT females (Fig. 2A). In contrast, water intake was not significantly different between dy2J/dy2J and WT males (Fig. 2A). Interestingly, metformin treatment slightly increased water intake in dy2J/dy2J females and also in WT males (Fig. 2A). Food intake was not significantly different between WT and dy2J/dy2J mice, neither in males nor in females and metformin did not alter food intake in any of the groups (Fig. 2B).
An 87% reduction in weight gain was observed in dy2J/dy2J females when compared with WT females whereas the reduction in males was around 54% (Fig. 2C). Metformin treatment had a positive effect in both dy2J/dy2J females and males, but was more explicit in dy2J/dy2J females, with levels statistically indistinguishable from those of WT mice (Fig. 2C). Accordingly, energy efficiency (calculated based on the weight gain and energy intake along the experimental period15) was positively affected by metformin with a six-fold increase (statistically significant) in dy2J/dy2J females and a 1.7-fold increase (non-significant) in dy2J/dy2J males (Fig. 2D).
Metformin treatment does not enhance muscle weight but augments forelimb grip strength in female dy 2J /dy 2J mice
Next, we evaluated if the weight gain was associated with larger skeletal muscles in metformin-treated dy2J/dy2J mice. A significant decrease in the weight of gastrocnemius (41% in females and 38% in males), tibialis anterior (23% in females and 36% in males) and quadriceps (30% in females and 37% in males) muscles was seen in dy2J/dy2J mice (Fig. 3A–C). Also, heart muscle weight was significantly declined in dy2J/dy2J mice (32% in females and 14% in males) (Fig. 3D). In contrast, there was no difference in the weight of dy2J/dy2J soleus muscle (neither in males nor females) compared to WT soleus muscle (Fig. 3E). Metformin treatment had no major effect in improving muscle mass in female and male dy2J/dy2J mice (Fig. 3), except for a slightly increased heart muscle weight in dy2J/dy2J females (Fig. 3D).
Yet, metformin significantly enhanced forelimb grip strength in dy2J/dy2J mice. Forelimb grip strength was significantly reduced (about 70%) in dy2J/dy2J females compared to WT females and metformin treatment approximately doubled the forelimb muscle strength (Fig. 4). Following this pattern, a 30% decrease in forelimb grip strength was observed in dy2J/dy2J males when compared with WT (but this reduction was not statistically different). Furthermore, metformin treatment marginally improved forelimb grip strength in both WT and dy2J/dy2J males (but again, the increase was not statistically different).
Dy2J/dy2J mice display significant hindlimb paralysis and similar hindleg lameness was noted upon metformin treatment (data not shown). Also, exploratory locomotion was evaluated but metformin did not confer any beneficial effect in dy2J/dy2J females or males (Supplemental Fig. S1).
Skeletal muscle histology is improved in female dy 2J /dy 2J mice
A microscopic evaluation of H&E-stained quadriceps muscle sections revealed typical muscular dystrophy characteristics with fiber degeneration/regeneration (evidenced by central nucleation) and fiber size variability in dy2J/dy2J mice (Fig. 5A). Central nucleation was significantly amplified in both dy2J/dy2J females and males and metformin treatment reduced the number of fibers with centrally located nuclei to levels statistically indiscernible from those of WT mice (Fig. 5B). In dy2J/dy2J female muscle, the percentage of fibers with cross sectional areas in the range of 500 to 1000 mm2 was robustly increased compared to WT counterparts. In contrast, the percentage of fibers between 2000 to 3000 mm2 was significantly decreased (Fig. 5C). Similarly, the percentage of fibers with cross sectional areas in the range of 500 to 1000 mm2 was increased compared to WT counterparts in dy2J/dy2J male muscle and the percentage of fibers between 2500–3500 was decreased (although statistical significance was not reached in the 2500–3000 intervals) (Fig. 5C). Notably, metformin treatment caused a shift of fiber size distribution and normalized the fiber size and proportion in dy2J/dy2J females (Fig. 5C) whereas metformin did not affect fiber size distribution in dy2J/dy2J males (Fig. 5C).
Pathological fibrosis is also a typical feature of dy2J/dy2J muscle and we quantified collagen content by sirius red and fast green staining. A similar collagen accumulation was noted in dy2J/dy2J females and males, but metformin treatment did not reduce total collagen deposition (Fig. 5D).
To further analyze connective tissue infiltration, we measured the relative gene expression of three different fibrosis-related genes; Fn1 encoding fibronectin; Col3a1 encoding the α1 subunit of collagen III and Tgfb1 encoding TGF-β1 in quadriceps and tibialis anterior muscles. We found that the expression of Fn1 was significantly increased in dy2J/dy2J female and male quadriceps and tibialis anterior muscles. Notably, metformin normalized Fn1 expression in female and male dy2J/dy2J quadriceps muscle and reduced it in dy2J/dy2J tibialis anterior muscle (Figs 6A and 7A). Similarly, Col3a1 expression was significantly increased in dy2J/dy2J female and male quadriceps and tibialis anterior muscles and metformin significantly reduced it in female and male dy2J/dy2J quadriceps muscle (but to a lesser extent in dy2J/dy2J tibialis anterior muscle) (Figs 6B and 7B). The expression of Tgfb1, on the other hand, remained enhanced in dy2J/dy2J female and male quadriceps and tibialis anterior muscles upon metformin treatment (apart from a slight reduction in dy2J/dy2J tibialis anterior) (Figs 6C and 7C). All in all, the gene expression analysis indicates that metformin affects some aspects of pathological fibrosis in dy2J/dy2J skeletal muscle.
To further assess whether metformin impacts muscle atrophy, we measured the relative gene expression of MurF1 encoding muscle RING-finger protein-1 and MAFbx encoding muscle atrophy F-box protein (two E3 ubiquitin ligases that are key regulators of muscle atrophy16. We found that expression of MuRF1 and MAFbx is enhanced in 10-week-old dy2J/dy2J tibialis anterior muscle from both males and females but not in corresponding quadriceps muscles and metformin did not alter the expression (except for increasing MAFbx expression in dy2J/dy2J female tibialis anterior muscle) (Figs 6D,E and 7D,E).
Finally, we measured the expression of Pgc1α encoding peroxisome proliferator-activated receptor gamma coactivator 1α (a key regulator of mitochondrial metabolism) that we have shown to be decreased in LAMA2-CMD patient myotubes5. However, expression of Pgc1α was not altered in dy2J/dy2J muscles and metformin did not change its expression either (Figs 6F and 7F).
Lastly, to investigate whether metformin treatment affected fiber type composition, we analyzed the expression of myosin heavy chain (MyHC), type 1 that is expressed in slow twitch fibers (Fig. 8A). The number of fibers with positive staining was slightly reduced in dy2J/dy2J mice (both females and males) compared to WT and metformin seemed to increase the percentage of positive slow twitch fibers in both WT and dy2J/dy2J animals. Even though metformin treatment tended to increase expression of MyHC, type 1, there were no significant differences between any of the four studied groups (Fig. 8B).
Metformin treatment enhances the weight of white adipose tissue in dy 2J /dy 2J mice
Finally, to investigate whether metformin influenced non-muscle organs we analyzed the weights of white adipose tissue, brown adipose tissue, liver, kidney and spleen. In dy2J/dy2J mice we noted a 60% and 54% decrease of white adipose tissue weight in females and males, respectively, and metformin treatment enhanced dy2J/dy2J white adipose tissue weight in both females and males but did not impact WT white adipose tissue weight (Fig. 9A). The weight of brown adipose tissue was not affected in dy2J/dy2J females but was significantly reduced in dy2J/dy2J males. Metformin did not alter the weight of brown adipose tissue in female mice but marginally increased the brown adipose tissue weight in dy2J/dy2J males (Fig. 9B). Neither liver nor kidney weights were affected in dy2J/dy2J females and males (Fig. 9C,D). Spleen weight, on the other hand, was reduced by 20% in dy2J/dy2J females (a non-significant reduction was also noted in dy2J/dy2Jmales) and metformin slightly increased spleen weight in dy2J/dy2J females (Fig. 9E).
Discussion
LAMA2-CMD, the second-most common form of congenital muscular dystrophy, remains incurable despite the development of successful genetic and pharmacological preclinical treatment strategies1,2. Metformin is FDA approved for treatment of type II diabetes in children over 10 years of age but is also used off-label to treat obesity in adolescents17. In this study, we show that metformin enhances weight gain, restores energy efficiency, augments muscle function, improves morphological features of muscular dystrophy and reduces the expression of some fibrosis-related genes in dy2J/dy2J females (and to a lesser extent in dy2J/dy2J males). Some features were not improved, such as exploratory locomotion but this may be due to the fact that metformin had no effect on hindleg lameness. Peripheral neuropathy is particularly evident in dy2J/dy2J mice but is not a clinical manifestation in patients18. Another therapeutic candidate for LAMA2-CMD is losartan that was demonstrated to confer clinical improvement and reduce fibrosis in the dy2J/dy2J mouse model19. The anti-apoptotic compound omigapil also provided beneficial effects in dy2J/dy2J mice20 as well as in the dyW/dyW mouse21 (another mouse model of LAMA2-CMD). Losartan and omigapil are expected to target fibrosis and apoptosis, respectively, but neither compound has been shown to regulate metabolism, which is significantly altered in LAMA2-CMD as well as in other muscular dystrophies5,22,23.
Metformin has been described to control metabolism, for example by decreasing lipogenesis and gluconeogenesis and modulating mitochondrial function24. Studies demonstrate that metformin increases healthspan and lifespan in mice25 and in elderly patients, improving glucose tolerance and regulating expression not only of metabolic genes but also collagen and DNA repair-related-genes in muscle and adipose tissue7. Moreover, it was recently described that metformin treatment attenuated fibrosis and insulin resistance in adipose tissue caused by doxorubicin treatment in rats26. In skeletal muscle, metformin treatment improved the oxidative metabolism in gastrocnemius muscle from mice and reduced muscle injury induced by cardiotoxin9. In the same study, reduction of cell damage and necrosis was demonstrated in vitro using C2C12 myotubes subjected to metformin treatment9. Finally, in tumor-bearing animals metformin was shown to increase protein synthesis and reduces protein degradation in gastrocnemius muscle27. It is plausible that metformin improves oxidative metabolism, which is significantly impaired in LAMA2-CMD cells5. On the other hand, while metformin conferred positive effects in mdx skeletal muscle, no clear protective actions on dystrophic metabolism were observed8. Hence, the mechanisms of metformin action in dy2J/dy2J mice are still to be clarified. Yet, it is interesting to note that another compound (N-acetyl-cysteine), which affects metabolism by reducing the formation of reactive oxygen species that may arise through insufficient mitochondrial respiration, also improves muscle function and structure in dy2J/dy2J mice (Harandi et al., unpublished data). Nevertheless, there are some other limitations of this study. For example, treatment was initiated at 6-weeks of age when muscle impairment is already readily detected in dy2J/dy2J mice14. It would be interesting to start metformin treatment sooner and continue over a longer period of time (as no overt side effects of metformin were noted). Moreover, we have only analysed histology of one skeletal muscle (quadriceps).
It is well known that gender affects a wide variety of physiological functions including cardiovascular and autoimmune systems and influences a broad range of diseases such as gastrointestinal, liver, kidney, endocrine, blood and neurological disorders. Moreover, gender also impacts pharmacokinetics and pharmacodynamics28. Duchenne muscular dystrophy is an X-linked recessive muscular dystrophy, thus affecting only boys, but apart from gender differences due to the genetic inheritance pattern, there is only little data available regarding gender differences in disease progression of muscular dystrophy29,30,31. Bearing this in mind, we monitored the disease progression during four weeks in 6-week-old dy2J/dy2J females and males. We found several important gender differences (at least in this age range). Weight gain was lower in dy2J/dy2J female mice compared to dy2J/dy2J males; water intake was significantly reduced in dy2J/dy2J females compared to WT females (not in corresponding males); a larger decrease of relative grip strength was noted in dy2J/dy2J females compared to dy2J/dy2J males and the shift in fiber size distribution was much more pronounced in dy2J/dy2J females compared to males. Thus, to our knowledge, this is the first time gender differences are reported in LAMA2-CMD.
Energy efficiency is known as the balance between energy intake and expenditure and is determinant to muscle mass maintenance15,32,33. Factors such as increased resting energy expenditure and uncoupled systems can lead to a metabolic inefficiency and consequently muscle loss, independently of energy intake33. It is known that metformin activates AMP-activated protein kinase, a critical sensor in the cell that regulates cellular metabolism, leading to an increase of catabolic pathways to produce ATP and a decrease of anabolic pathways that consume ATP, equilibrating the energy stores in cells24,34,35. In muscular dystrophies, food and drink ingest can be impaired by dysphagia and tend to worsen during disease progression36,37,38,39. Our data shows that even though food intake was not altered in dy2J/dy2J female and male mice, the energy efficiency was drastically decreased, being most prominent in females with negative values compared to WT mice (which we considered 100% efficient). Notably, metformin was able to improve energy efficiency by 60% in dy2J/dy2J females and 20% in dy2J/dy2J males, again supporting the beneficial effects of treatment by gender.
In conclusion, this study shows that metformin treatment significantly reduces muscular dystrophy in dy2J/dy2J females. Importantly, metformin is already approved for use in humans, which is advantageous from a clinical point of view. Nevertheless, metformin treatment does not target the primary genetic defect and is not expected to completely cure LAMA2-CMD. Yet, metformin could be used as a supportive treatment that may improve many of the pathological symptoms in LAMA2-CMD.
Materials and Methods
Animals
Heterozygous dy2J/dy2J (B6.WK-Lama2dy-2J/J) were obtained from Jackson Laboratory and bred and maintained in our animal facility to generate dy2J/dy2J and WT mice according to institutional animal care guidelines. All experimental procedures involving animals were approved by the Malmö/Lund (Sweden) ethical committee for animal research (ethical permit number 5.8.18–02255/2017) in accordance with guidelines issues by the Swedish Board of Agriculture. The animals were maintained at 22 ± 2 °C with a regular light-dark cycle (light on from 6:00 am to 6:00 pm) and had free access to food and water. The diet consisted of 51.2% carbohydrate, 22% protein and 4.25% fat (Special Diet Services). Six-week-old mice were separated according to gender and into wild-type (WT) and dystrophic (dy2J/dy2J) groups and further randomly subdivided into WT control (7 females and 13 males), WT metformin-treated (10 females and 6 males), dy2J/dy2J control (5 females and 4 males) and dy2J/dy2J metformin-treated (5 females and 5 males) groups. Thus, mice were housed according to genotype, gender and treatment. Mouse numbers vary slightly for the different outcome measures and we refer to further information in the figure legends.
Treatment
Metformin (CAS 1115-70-4, Calbiochem) diluted in filtered water was administrated by oral gavage once a day at 250 mg/kg body weight during four weeks. Control animals received filtered water by oral gavage. Initial and final body weight and weight gain were analyzed. Food and water intake were estimated twice-a-week (by weight measurements divided by number of animals in cage). The energy efficiency ratio was calculated as the weight gain (g) during the experimental period divided by the cumulative energy intake over the same period (kcal), according to Jung et al.15.
Locomotor activity and grip strength
After four weeks of treatment, animals were subjected to exploratory locomotion testing and grip strength analysis. Exploratory locomotion was evaluated in an open-field test, as previously described40. In each experiment, a mouse was placed into a new cage and allowed to explore the cage for five minutes. The time that the mouse spent moving around was measured.
Forelimb grip strength was measured using a grip-strength meter (Columbus Instruments) as previously described40. In brief, the mouse was held by the base of the tail and allowed to grasp the flat wire mesh of the pull bar with its forepaws. When the mouse got a good grip, it was slowly pulled away by its tail until it released the pull bar. Each mouse was allowed to pull the pull bar five times. The two lowest values were rejected and the mean of the three remaining values was counted. Animals were not subjected to any training prior to the experiment. Grip strength was calculated as force divided by final body weight41.
Tissue collection
Mice were sacrificed by cervical dislocation. Tissues were rapidly excised, carefully dissected, and weighed. Skeletal muscles isolated were soleus, gastrocnemius, tibialis anterior and quadriceps. Heart, white adipose tissue (perigenital), brown adipose tissue (intercostal), liver, spleen and kidney were isolated and weighed as well.
Histology and immunohistochemistry
For morphometric analyses, quadriceps muscles were either embedded in OCT compound (Tissue-Tek) and frozen in liquid nitrogen or embedded in paraffin. Paraffin-embedded specimens were sectioned using a microtome (5 μm) (Microm H355) and OCT embedded sections were sectioned using a cryostat (7 μm) (Microm HM 560). Paraffin sections were stained with hematoxylin and eosin (H&E) staining and cryosections were subjected to immunostaining (see further down). H&E stained sections were scanned using an Aperio ScanScope CS2 scanner with ScanScope console version 8.2.0.1263. Central nucleation was quantified using ImageJ software version 1.43 u, Cell Counter plug-in (NIH). The whole quadriceps cross-section muscle was used for quantification and the percentage of central nuclei was subsequently calculated. The fiber area of biotinylated wheat germ agglutinin (WGA) stained muscle fibers was measured and quantified using ImageJ and approximately 300 fibers per mouse were analyzed.
Immunohistochemistry was performed as previously described42 using a monoclonal antibody against anti-myosin (skeletal, slow; M8421, Sigma Aldrich). In addition, biotinylated WGA was used as a membrane marker. The secondary antibody was goat anti-mouse IgG 546 (Thermo Fisher Scientific) together with avidin. The slides were analyzed by Zeiss Axioplan fluorescence microscope (Zeiss) and images were captured using an ORCA 1394 ER digital camera (Hamamatsu Photonics) and Openlab software version 4 (Improvision). The whole quadriceps cross-section muscle was used for quantification and the percentage of fibers with positive MyHC type 1 staining was subsequently calculated (by visual assessment).
Sirius red/fast green quantification
Collagen content was quantified by a colorimetric method as previously described43. Around 15 paraffin sections (15 μm) were placed in a plastic tube. Paraffin removal was accomplished by immersing the sections in xylene (5 min), xylene/ethanol (1:1) (5 min), ethanol (5 min), ethanol/water (1:1) (5 min) and water (5 min). The sections were then stained with fast green/sirius red for 30 minutes on rotation and subsequently washed with distilled water until excess dye was removed and solution was clear. One ml of 0.1 N NaOH was added and the solutions were analyzed by absorbance at 560 nm and 605 nm.
Real time PCR analysis
RNA isolation was performed by using RNeasy Fibrous Tissue Kit QIAGEN according to manufacturer’s recommendations. First-strand cDNA was synthesized from total RNA (0.8 μg) with oligonucleotide dT15 primers and random primers p(dN)6 by use of First Strand cDNA synthesis kit (Roche). Real time-PCRs were performed using Light Cycler 480 SYBR Green Master I (Roche) and were analyzed by Light Cycler 480 SW 1.5 software (Roche). Oligonucleotide sequences used for PCR are listed in Supplementary Table S1. Primers were from Sigma (KiCqStart SYBR Green Primers) or designed as previously described44 using Primer3 software (http://primer3plus.com/cgi-bin/dev/primer3plus.cgi, last accessed, Aug 10, 2018). Primer parameters were defined as follows: product size: 50–150 bp; primer size: 18–22 bp (opt: 20); primer Tm: 57–63 °C (opt: 60); primer GC%: 40–60%; maximum self-complementarity: 3–4. The Operon tool (http://www.operon.com/tools/oligo-analysis-tool.aspx, last accessed Aug 10, 2018) was utilized to check the putative primer-dimer formation. Amplification conditions consisted of 5 s of denaturation at 94 °C, 9 s of annealing at 55–60 °C and 9 s of extension at 72 °C for each step for 45 cycles. The relative amount of all mRNAs was calculated using the comparative CT method. Rplp0 was used as the invariant control.
Statistical analysis
All data are shown as mean ± S.E.M. Statistical analysis of the data was performed by means of two-way analysis of variance (ANOVA) with Bonferroni post hoc test for comparison of gender and disease effect and one-way analysis of variance (ANOVA) with Bonferroni post hoc test for comparison of treatment effect. p < 0.05 values were considered as statistically significant.
Data Availability
The datasets generated during and/or analyzed during the current study are available from the corresponding authors on reasonable request.
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Acknowledgements
This work was generously supported by Anna and Edwin Berger Foundation, Anna-Greta Crafoord Foundation for Rheumatology Research, Association Française contre les Myopathies, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Crafoord Foundation, Fanny Ekdahl Foundation, Greta and Johan Kock Foundation, Jane and Dan Olsson Foundation, Lisa and Johan Grönberg Foundation, Neuroförbundet, Olle Engkvist Byggmästare Foundation, Royal Physiographic Society in Lund, The Swedish Research Council, Tore Nilsson Foundation and Österlund Foundation. We dedicate this work in the memory of our friend and colleague Bruno Menezes de Oliveira, Ph.D.
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C.C.F.-O. and M.D. designed research; C.C.F.-O., B.M.S.O., Z.K. and V.M.H. performed research and analyzed data; C.C.F.-O. and M.D. wrote the paper.
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Fontes-Oliveira, C.C., M. Soares Oliveira, B., Körner, Z. et al. Effects of metformin on congenital muscular dystrophy type 1A disease progression in mice: a gender impact study. Sci Rep 8, 16302 (2018). https://doi.org/10.1038/s41598-018-34362-2
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DOI: https://doi.org/10.1038/s41598-018-34362-2
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