Abstract
The HBV core protein self-assembles into particles and encapsidates immune-stimulatory bacterial RNA through a cationic COOH-terminal (C150–183) domain. To investigate if different cationic domains have an impact on the endogenous RNA-binding of HBV-C antigens in mammalian cells, we developed a strep-tag (st) based expression/purification system for HBV-C/RNA antigens in vector-transfected HEK-293 cells. We showed that HBV-stC but not HBV-stC149 particles (lacking the cationic domain) capture low amounts of mammalian RNA. Prevention of specific phosphorylation in cationic domains, either by exchanging the serine residues S155, S162 and S170 with alanines (HBV-stCAAA) or by exchanging the entire cationic domain with a HIV-tat48–57-like sequence (HBV-stC149tat) enhanced the encapsidation of RNA into mutant core particles. Particle-bound mammalian RNA functioned as TLR-7 ligand and induced a Th1-biased humoral immunity in B6 but not in TLR-7−/− mice by exogenous (protein) and endogenous (DNA) vaccines. Compared to core particles, binding of mammalian RNA to freely exposed cationic domains in assembly-deficient antigens was enhanced. However, RNA bound to non-particulate antigens unleash its Th1-stimulating adjuvant activity by DNA- but not protein-based vaccination. Mammalian RNAs targeted by an endogenously expressed antigen thus function as a natural adjuvant in the host that facilitates priming of Th1-biased immune responses by DNA-based immunization.
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Introduction
Plasmid DNA vaccination is an attractive technique to induce antigen-specific humoral and cellular immune responses1,2. Vector-encoded antigens are expressed in in vivo transfected APCs of the host. Antigens and/or antigenic material, released from non-professional antigen-expressing APCs (e.g., myocytes) to professional APCs (e.g. DCs) facilitated priming of immune responses3. Multiple laboratories have identified methods to optimize vector-driven transcription and protein expression, and/or to improve the immunogenicity of DNA-encoded antigens by co-delivering immune stimulating adjuvants, co-expressing immune modulatory cytokines or molecules stimulating the innate immune system2. In particular, the local induction of an inflammatory milieu at the side of vaccine injection was thought to attract professional APCs and thereby favour de novo priming of Th1-biased immune responses4.
The innate immune system has evolved endo/lysosomal and cytoplasmic pattern recognition receptors (PRRs) for the detection of pathogen-associated molecular patterns (PAMPs) like foreign nucleic acids or conserved molecules and structures of an invading pathogen5. Toll-like receptors (TLRs) can discriminate between different microbial nucleic acids, such as double-stranded (ds) RNAs (recognized by TLR-3), single-stranded (ss) RNAs (recognized by TLR-7 or TLR-8) or bacterial DNAs containing unmethylated CpG motifs (recognized by TLR-9). Nucleic acid-sensing TLRs are sequestered in an endo/lysosomal compartment and induce complex signaling cascades that result in the production of proinflammatory cytokines, chemokines and type I interferons5. These signals attract and activate professional APCs (DCs), which play a crucial role for priming adaptive immune responses4,6. Under certain conditions, mammalian self RNAs can also stimulate TLR-3- and/or TLR-7-mediated immune responses, for example Sm/RNP associated with lupus autoantigens7,8,9 or after release from damaged cells10. We thus hypothesized that cellular RNAs are potent “helper” molecules which, when captured by vector-expressed endogenous antigens may function as molecular adjuvant11,12,13.
An attractive antigen for targeting an endogenous, RNA-based adjuvant activity is the HBV core (HBV-C) protein. HBV-C self-assembles into particles that encapsidate nucleic acids, in particular pregenomic RNA (pgRNA) in HBV-infected cells or non-specific heterologous RNA in bacterial or yeast expression systems14,15 through a cationic COOH-terminal (C150–183) domain. Heterologous bacterial RNA bound to recombinant HBV-C particles specifically stimulates the innate immune system via the TLR-7 and induces a vigorous Th1-biased serum antibody response in mice16,17. Similarly, bacterial RNA encapsidated into recombinant bacteriophage virus-like particles displaying peptides of the human papillomavirus capsid protein L2 induces a Th1-biased serum antibody response in mice18. Particle-bound bacterial RNA has a >1000-fold higher potency as a Th1-inducing adjuvant than free RNA mixed to a protein antigen16. This suggested that encapsidation of heterologous RNA in particulate structures is a prerequisite for delivering RNA into APCs of the host. Furthermore, there is evidence that RNA bound to the cationic C150–183 domain of endogenously expressed HBV-C particles in mammalian cells exert a specific adjuvant activity: HBV-C, but not HBV-C149 and HBV-E antigens (lacking the cationic domain) bound [3H]-uracil-labelled cellular RNA in vector-transfected cells and stimulated a Th1-biased core-specific humoral immunity in mice by DNA vaccination with the gene gun16.
In this study, we investigated the non-specific binding of mammalian RNA to different cationic domains at the COOH terminus of HBV-C. We generated expression vectors that encode particle-forming and assembly-deficient HBV-C antigens and developed a strep-tag (st) based expression/purification system for HBV-C/RNA complexes in transiently transfected human HEK-293 cells. To elucidate the adjuvant activity of mammalian RNA captured by HBV-C antigens, we vaccinated B6 and TLR-deficient mice with recombinant antigens (exogenous antigens) or antigen-expressing vector DNA (endogenous antigens) and determined priming of Th1/Th2-biased core-specific antibody responses.
Results
HBV core antigens containing different cationic domains self-assemble into particles that capture mammalian RNA
The HBV-C protein contains a 34-residue cationic domain (C150–183) at its COOH-terminus (Fig. 1a). HBV-C and HBV-C149 (lacking the cationic domain) proteins self-assemble into particles, but only HBV-C particles non-specifically encapsidate RNA in bacterial expression systems19. We hypothesized that HBV-C may also bind mammalian RNA when selectively expressed in cells transfected with recombinant vector DNA or, relevant for DNA-based immunization, expressed in murine APCs targeted by plasmid DNA injection. To investigate particle formation and RNA-binding of different HBV core antigens under standardized conditions, we developed an expression system in human HEK-293 cells that allowed affinity purification of proteins under physiological conditions20. We used a HBV-C expressing pCI/C vector and cloned a streptavidin-binding tag (strep-tag or st) NH2-terminally to the HBV-C sequence. This generated the eukaryotic pCI/stC expression vector (Fig. 1a). Both, pCI/C and pCI/stC vectors expressed comparable levels of HBV-C and HBV-stC antigens in transiently transfected HEK-293 cells (Fig. 1b; Supplementary Figs S1 and S2), indicating non-disturbed expression of the HBV-stC protein by its NH2-terminal strep-tag modification.
For large-scale production, we transiently transfected 5–10 × 108 HEK-293 cells with the pCI/stC vector. Cells were lysed 48 hours post transfection. The HBV-stC fusion protein was precipitated using a StrepTactin sepharose-packed column and eluted with desthiobiotin20. SDS-PAGE analysis of purified samples revealed a single HBV-stC protein band in the different fractions (Fig. 1c, lanes 1–8). The NH2-terminal strep-tag was thus accessible for recognition by the StrepTactin sepharose matrix and could be used to purify the HBV-stC protein (to >97% purity) from cell lysates. Electron microscopy of purified HBV-stC confirmed its particulate structure (Fig. 1d). Mammalian RNA (stained with ethidium bromide, EB) co-migrated with HBV-stC particles (stained with Coomassie Blue, CB) in native agarose gels (Fig. 1e, left panels; Supplementary Fig. S3a), demonstrating a tight interaction between particles and RNA. However, HBV-stC particles produced in HEK-293 cells contained lower amounts of heterologous RNA than HBV-stC particles expressed in bacteria (Fig. 1e, right panel; Supplementary Fig. S3b). The HBV-stC149 protein (lacking the cationic COOH-terminus) was efficiently expressed in transiently transfected HEK-293 cells but did not bind RNA (Supplementary Figs S2 and S4). Purified HBV-stC preparations almost quantitatively contained particles that did not migrate into naïve polyacrylamide gels under non-denaturing conditions (Supplementary Fig. S5). In contrast, HBV-stC149 preparations contained particles, but also a considerable proportion of non-particulate antigen migrating into the gels (Supplementary Fig. S5). As compared to HBV-stC particles, HBV-stC149 particle formation and/or stability was thus disturbed in mammalian HEK-293 cells.
The cationic C150–183 domain contains three repeated SPRRR motifs and specific phosphorylation of these serine residues (at positions S155, S162 and S170) could affect the RNA-binding to HBV-stC particles21,22,23. We exchanged the serine residues S155, S162 and S170 in the C150–183 domain with alanine residues (mimicking a non-phosphorylated state) or with glutamic acid and aspartic acid residues (mimicking a phosphorylated state) (Fig. 1f). This generated the pCI/stCAAA, pCI/stCEEE and pCI/stCDDD vectors, which were used to produce core particles in transiently transfected HEK-293 cells (Fig. 1f–h). Comparable with HBV-stC particles, HBV-stCEEE and HBV-stCDDD particles contained low amounts of cellular RNA (Fig. 1e,h). In contrast, purified HBV-stCAAA particles bound significant higher amounts of RNA than HBV-stC or mutant HBV-stCEEE and HBV-stCDDD particles (Fig. 1e,h; Supplementary Fig. S6). Silencing specific phosphorylation sites in the C150–183AAA domain thus substantially enhanced the encapsidation of mammalian RNA into endogenously expressed particles in vivo in vector-transfected cells.
To investigate whether the C150–183 sequence is crucial to bind mammalian RNA, we exchanged this domain with a short cationic HIV-tat48–57-like sequence (GRKKRRQRRRRRRQ; [www.uniprot.org/uniprot/P04610]) lacking any phosphorylation sites. This generated the pCI/stC149tat vector (Fig. 2a). The HBV-stC149tat antigen was isolated from transiently transfected HEK-293 cells with high purity, self-assembled into particles which captured high amounts of mammalian RNA (Fig. 2b–d; Supplementary Fig. S3a). This allowed us to analyze molecular and immune-stimulating features of the encapsidated RNA. Particle-bound nucleic acids were eliminated by RNase A but remained readily detectable after DNase I treatment (Fig. 2e), confirming the selective binding of RNA to HBV-stC149tat particles. Mammalian RNAs extracted from HBV-stC149tat particles varied in length from about 50 to 4000 nucleotides with no specific prevalence for small or large RNAs (Fig. 2f). The prominent ribosomal rRNA forms present in HEK-293 cells were not preferentially bound by HBV-stC149tat particles (Fig. 2f). This showed that HBV-stC149tat particles non-specifically captured mammalian RNAs via the cationic HIV-tat48–57-domain.
Particle-bound mammalian RNA stimulates a Th1-biased core-specific humoral immune response by exogenous protein vaccination
Bacterial RNA bound to recombinant HBV-C particles induced a core-specific, Th1-biased IgG2 antibody response in mice16,17,19. Similarly, a single injection of HBV-stC149tat particles (containing high amounts of mammalian RNA) preferentially induced core-specific Th1-biased IgG2b antibodies (IgG1/IgG2b ratios of ≤0.25) in C57Bl/6J (B6) mice (Fig. 3a,b). In contrast, RNA-free HBV-stC149 (Supplementary Fig. S4) preferentially induced IgG1 antibodies (IgG1/IgG2b ratio ≥5) (Fig. 3a,b). HBV-stC149tat antigen induced significant higher core-specific IgG antibodies than HBV-stC149 (Fig. 3a). The polarization of the core-specific immune responses was further confirmed by the inducible IFN-γ release of activated CD4+ T cells16,17. A prominent IFN-γ release was induced in T-cell preparations of HBV-stC149tat- but not HBV-stC149-immune or non-immunized mice upon in vitro stimulation with a well-defined I-Ab-binding (C128–140; TPPAYRPPNAPIL) CD4+ T-cell epitope of HBV core (Fig. 3c), confirming that the antibody isotype profile is a reliable indicator for Th1/Th2-mediated immune responses in mice24.
Bacterial RNA bound to recombinant HBV-C particles induced a Th1-biased IgG2 serum antibody response in TLR-7+ mice, but a decreased and balanced IgG1/IgG2 response in TLR-7−/− mice17. Similarly, the IgG isotype profiles of the core-specific serum antibody responses induced by HBV-stC149tat particles differed in B6 and TLR-7−/− mice (Fig. 4). A single injection of HBV-stC149tat particles induced higher core-specific IgG antibody titers in B6 mice than in TLR-7−/− mice (Fig. 4a). HBV-stC149tat particles induced a balanced core-specific IgG1/IgG2b antibody response (IgG1/IgG2b ratios of 1 ± 0.15) in TLR-7−/− mice (Fig. 4), but comparable Th1-biased antibody responses (IgG1/IgG2b ratios of ≤0.3) in TLR-3−/− and B6 mice (Supplementary Fig. S7; Figs 3 and 4). In contrast, the HBV-stC149 vaccine induced core-specific Th2-biased IgG1 antibody responses (IgG1/IgG2b ratios of ≥5) in both, TLR-7−/− and B6 mice (Supplementary Fig. S7; Fig. 3). Mammalian RNA captured by HBV-stC149tat particles thus stimulated a Th1-biased, TLR7-mediated humoral immunity by exogenous protein vaccination.
Mammalian RNAs bound to non-particulate core antigens do not stimulate Th1-biased humoral immune responses by exogenous (protein) vaccination
We next asked if encapsidation of mammalian RNA into particles is a prerequisite to elicit Th1-biased antibody responses by exogenous protein vaccination and if freely exposed cationic domains in assembly-deficient core antigens have an impact on the net RNA binding of mutant core antigens. Therefore, we constructed assembly-deficient HBV core variants encoding different cationic domains (C150–183, C150–183AAA or HIV-tat48–57) by exchanging the thyrosine (Y) at position 132 with an alanine (A) residue25. This generated the pCI/stCY132A, pCI/stCAAAY132A and pCI/stC149-tatY132A vectors, respectively (Fig. 5a). All antigens were efficiently expressed in transiently transfected HEK-293 cells and we could not detect particulate structures in any of these preparations by electron microscopy (data not shown). Non-particulate antigens showed a clear increase in the RNA binding between stCY132A and stCAAAY132A and between stCAAAY132A and stC149-tatY132A (Fig. 5b). This confirmed above findings that prevention of specific phosphorylation in cationic C150–183 domain and, in particular, the non-phosphorylatable cationic tat domain enhanced binding and encapsidation of cellular RNAs into particles (Figs 1 and 2). However, non-particulate antigens were associated with cellular proteins that co-purified in our expression system (Fig. 6a; Supplementary Fig. S8). In particular, the HBV-stC149tatY132A antigen with the highest RNA binding capacity (Fig. 5b) stable bound high amounts of cellular proteins (Supplementary Fig. S8).
To directly compare the RNA-binding and immunogenicity of non-particulate and particulate antigens, we used HBV-stCAAAY132A and HBV-stCAAA antigens showing intermediate RNA-binding (Figs 1 and 5). The non-particulate HBV-stCAAAY132A bound 5–10 fold higher amounts of mammalian RNA than HBV-stCAAA particles (calculated for the same amount of monomers), accompanied with an enhanced binding of cellular proteins (Fig. 6a,b; Supplementary Fig. S9). This suggested that the co-purified cellular proteins bind to cellular RNAs captured by the mutant cationic domain in the non-particulate HBV-stCAAAY132A antigen. MALDI-TOF-MS analyses (commercially performed by Toplab Corp., Planegg, Germany) identified the most prominent protein co-precipitating with HBV-stCAAAY132A as the 32 kDa mature form (residues 74–282) of the complement component 1 Q subcomponent-binding protein (C1QBP) also called gC1qR or HABP1 (Fig. 6a; Supplementary Fig. S10b)26. C1QBP binds to the cationic C150–183 domain of a monomeric HBV-P22 precore protein but not to particulate HBV-C protein27. RNase A treatment of HBV-stCAAAY132A preparations quantitatively destroyed the bound RNA and removed most of the co-precipitated cellular proteins (Supplementary Fig. S10). The binding of the C1QBP protein to mutant HBV-stCAAAY132A was resistant to RNase A treatment (Supplementary Fig. S10b), indicative for a protein-protein interaction.
To evaluate the antigenicity of particulate and non-particulate RNA-binding antigens, we vaccinated mice with the same amounts of recombinant HBV-stCAAA particles or HBV-stCAAAY132A antigen (calculated for the same amount of monomers). HBV-stCAAA particles induced significant higher core-specific IgG antibodies than non-particulate HBV-stCAAAY132A antigen (Fig. 6c). Comparable to HBV-stC149-tat particles, HBV-stCAAA particles induced a core-specific Th1-biased antibody response in B6 mice (Figs 3 and 6c,d), demonstrating that the vaccine-induced Th1-immunity was independent from the cationic motif and therefore primarily depends on the particle-bound RNA. In contrast, the non-particulate HBV-stCAAAY132A antigen, while binding higher amounts of RNA than HBV-stCAAA particles, induced an almost exclusive Th2-biased IgG1-specific antibody response (IgG1/IgG2b ratios of ≥15; Fig. 6c,d). Encapsidation of cellular RNA into particles thus facilitated induction of a Th1-biased humoral immunity in mice by exogenous protein immunization.
Murine RNAs captured by endogenously expressed particles or non-particulate core antigens stimulate a Th1-biased humoral immunity by DNA vaccination
Endogenously expressed HBV-C, but not HBV-C149 and HBV-E induced a Th1-biased humoral immunity in H-2d BALB/c mice vaccinated with low amounts (1–2 µg) of vector DNA with the gene gun16. Similarly, the pCI/stC149tat and pCI/stC149 vectors induced Th1- (IgG1/IgG2b ratios of <0,4) and Th2-biased core-specific antibody responses (IgG1/IgG2b ratios of >10) in H-2b B6 mice, respectively and the total core-specific IgG response was significantly enhanced in pCI/stC149tat-immune B6 mice (Fig. 7a,b). Furthermore, the pCI/stC149tat vector induced significant higher levels of core-specific IgG antibodies in B6 mice as compared to TLR-7−/− mice (Fig. 7c). Most interestingly, the pCI/stC149tat vector induced a balanced core-specific IgG1/IgG2b antibody profile (IgG1/IgG2b ratios of 1 ± 0.4) in TLR-7−/− mice (Fig. 7c,d). This showed that murine RNA directly captured in host APCs by endogenously expressed HBV-stC149tat particles operates as a TLR-7 ligand and stimulates a Th1-biased humoral immunity.
Furthermore, pCI/stCAAA and pCI/stCAAAY132A vectors, expressing RNA-binding particulate and non-particulate antigens, primed Th1-biased core-specific antibody responses (IgG1/IgG2b ratios of ≤0.35) in B6 mice (Fig. 7e,f). The pCI/stCAAAY132A vector expressed 2-3 fold higher steady state levels of core antigen than the pCI/stCAAA vector in transiently transfected cells (Supplementary Fig. S11), but endogenously expressed stCAAA particles induced higher core-specific IgG antibody titers than the non-particulate stCAAAY132A antigen (Fig. 7e). However, and in contrast to protein vaccines, the pCI/stCAAAY132A vector expressing the non-particulate stCAAAY132A antigen induced a Th1-biased core-specific antibody response (IgG1/IgG2b ratios of ≤0.25) (Figs 6c,d and 7e,f). Thus, the non-particulate stCAAAY132A antigen induced a Th1-biased core-specific antibody response by endogenous (DNA) but not exogenous (protein) vaccines.
Discussion
Chimeric HBV core particles displaying foreign epitopes or antigenic domains on their surface emerged as a platform technology for the induction of humoral immune responses by recombinant protein-based vaccines28. We here show novel features of HBV core antigen for its usage in DNA-based vaccines: First, different cationic domains located COOH-terminally to the same C1–149 antigen backbone non-specifically bound different amounts of mammalian RNAs in transiently transfected cells, and RNA-binding to both, particulate and non-particulate antigens was significantly enhanced by silencing and/or omitting specific phosphorylation sites in these domains. Second, mammalian RNAs encapsidated in HBV core particles, but not bound to non-particulate core antigens stimulated TLR-7-mediated, Th1-biased core-specific humoral immune responses by exogenous protein immunization. Third, endogenously expressed HBV core particles as well as non-particulate core antigens capture murine RNAs in in vivo transfected APCs that stimulate Th1-biased core-specific humoral immune responses by DNA immunization. We could show that induction of Th1-biased humoral immune responses by endogenously expressed RNA-binding particles critically depends on TLR-7 signaling. Mammalian RNAs targeted by an endogenously expressed vaccine antigen thus function as an endogenous adjuvant11,12,13 in the host, which facilitates priming of Th1-biased immune responses by DNA-based immunization.
The 183-residue HBV-C contains two well-defined domains: The NH2-terminal 149 aa comprise an assembly domain sufficient for particle formation and a COOH-terminal cationic domain (aa 150–183) that mediates binding of HBV-C to nucleic acids, in particular pregenomic RNA (pgRNA) in HBV-infected cells. Specific binding of viral pgRNA to HBV-C in an infected cell requires concerted interactions between the cationic HBV-C domain, the viral polymerase and a 5′ ε stem-loop in the pgRNA29,30,31. However, the mechanism(s) preventing the non-specific binding of cellular RNAs to the cationic C150–183 domain of HBV-C in HBV-infected cells are largely unknown. Non-specific binding of RNA may depend only on interactions between the positively charged, arginine-rich C150–183 domain and negatively charged RNA molecules. Synthetic HBV-C derived peptides C150–157 (RRRDRGRS) or C164–179 (SPRRRRSQSPRRRRSQ) tightly bound nucleic acids in vitro, indicating that the guanidinium head groups on the arginine-residues interact with the negatively charged phosphate backbone of RNA32,33. We here showed that identical HBV-stC particles bind low amounts of mammalian RNA, but high amounts of bacterial RNA (Fig. 1e; Supplementary Fig. S3). Non-specific incorporation of mammalian RNA into particles, but also binding of RNA to non-particulate core antigens in transfected cells was significantly enhanced by preventing the specific phosphorylation of the cationic domain, either by exchanging the entire cationic C150–183 domain with a cationic HIV-tat48-57-like domain (HBV-stC149tat) or by exchanging the serine residues at positions S155, S162 and S170 with alanine residues (HBV-stCAAA). It has been shown that the phosphorylation state of the cationic C150-183 domain, and in particular of the serine residues S155, S162 and S170 (or S157, S164 and S172 in the genotype A), is crucial for the specific encapsidation of viral pgRNA into HBV-C particles, reverse transcription and intracellular trafficking of capsids21,23,34,35,36. When these serines were exchanged with alanine residues viral pgRNA was not encapsidated into HBV-C particles21,37. This suggested that both, non-specific binding of cellular RNAs and specific binding of pgRNA to endogenously expressed HBV core antigens in vivo is regulated by the phosphorylation state of the C150–183 domain.
‘Exogenous’ bacterial17 and mammalian RNA bound to isolated HBV core particles functioned as TLR-7 ligand and stimulated Th1-biased antibody responses in mice. Usually, TLRs are not directly stimulated by microbial or mammalian nucleic acids, because their ligand-binding domains face into the lumen of the endo/lysosomal compartment5. Therefore, TLR-stimulating nucleic acids must be translocated from the cytosol and/or from the outside of a cell into the endo/lysosomal compartment. Disruption of core/RNA particles prevented the Th1-inducing adjuvant effect of bacterial RNA16. Similarly, a non-particulate HBV-stCAAAY132A antigen, while binding significant higher amounts of mammalian RNA than its particulate HBV-stCAAA form elicited a Th2-biased humoral immunity by protein vaccination. This indicated that encapsidation of heterologous RNA into HBV-C particles is crucial to protect the immune-stimulating RNA from rapid degradation after injection into mice and/or to deliver it into the TLR-7 expressing endo/lysosomal compartment of APCs. Similarly, antigen-encoding RNA condensed with a cationic protamine peptide stimulate TLR-7-dependent, Th1-biased adaptive immune responses by RNA-based vaccines38,39. Several virus-specific RNA motifs and polyuridylic (polyU) sequences that engage the TLR-7 receptor have been identified40. HBV-stC149-tat particles contained mammalian RNAs that varied in length from about 50 to 4000 nucleotides, but it is unknown whether a specific RNA species and/or specific motifs within these RNA molecules engage the TLR-7. It has been shown that B-cells specifically take up recombinant HBV-C particles17. Specific protein spikes on the surface of HBV-C particles could cross-link membrane-bound immunoglobulin on B-cells17. RNA-bound HBV-Core particles could activate B-cells by combined B-cell antigen receptor/TLR-7 engagement7,41. This could further explain the exceptional potent immunogenicity of exogenous RNA-bound HBV-C particles to trigger Th1-biased humoral immune responses. Non-particulate core antigens present with different cationic domains bound significant higher amounts of mammalian RNA, but also cellular proteins than their particulate forms. This suggested that freely exposed cationic domains in non-particulate antigens efficiently bound cellular RNA. However, we cannot exclude (and prevent) that the bulk of cellular RNA as well as cellular proteins stable bind to these non-particulate antigens during cell extraction. Thus, these ‘dirty’ non-particulate antigens are not attractive for their usage as recombinant vaccines.
Endogenously expressed intracellular or secreted antigens, e.g., HBV-surface, HBV-C149 and HBV-E that do not bind cellular RNA induced a Th1-biased humoral immunity in mice when vaccinated with high amounts (50–100 µg) of naked vector DNA, but a Th2-biased humoral immunity when vaccinated with low amounts (1–2 µg) of plasmid DNA by intradermal delivery with the gene gun16,42,43. This confirmed that high amounts of plasmid DNA itself, for example via binding to endogenous DNA sensor(s) that activate the TBK1-STING pathway5 induce Th1-stimulating innate signals in mice42. The only exception we have observed up to now was the priming of specific Th1-biased responses by gene gun vaccination of DNA expressing RNA-capturing core antigens (Fig. 7)16. Most interestingly, particle-bound RNA stimulated a Th1-biased immunity by both, protein and DNA vaccination, whereas RNA bound to non-particulate antigens almost exclusively induced Th2-biased immune responses by exogenous (protein) antigens and Th1-biased immune responses by endogenous (DNA) antigens. Furthermore, the total core-specific IgG antibody responses were significantly lower in mice vaccinated with RNA-bound non-particulate antigens either as protein or DNA vaccine. This indicated that core-specific antibodies directed against an immunodominant c/e1 epitope presented on the tip of protein spikes on the surface of HBV-C particles (i.e., the major immunodominant region between C78–82; MIR)44, were efficiently induced by particulate but not non-particulate antigens16. Neither HBV-Core particles nor non-particulate antigens used in this study contain transmembrane domains or enter the secretory pathway [www.uniprot.org/uniprot/P03146] and therefore are intracellular antigens. It is unclear how DNA vaccines prime antibody responses to intracellular antigens as B-cells require exogenous antigen for stimulation. Therefore, at least a small amount of antigen must be released from in vivo transfected antigen-expressing cells, for example, induced by cell death mechanisms3. However, the priming of Th1-biased antibody responses to RNA-bound non-particulate core antigens by DNA but not protein vaccines suggested that different mechanisms, local milieus and/or cell types are involved in antigen/RNA uptake by B-cells.
In this study, we focused on the induction of humoral immune responses by endogenously expressed core variants differing in particle formation and RNA binding. However, endogenously expressed RNA-capturing HBV-C particles also efficiently induced CD8+ T-cell responses and controlled HBV clearance in murine infection model45,46,47. Similarly, we could induce HBV-C but not HBV-surface- specific effector CD8+ T-cell responses in 1.4HBV-Smut tg mice that harbor a replicating HBV genome in hepatocytes by DNA-based vaccination48,49,50. HBV-C (Kb/C93)-specific CD8+ T-cells accumulated in the liver of pCI/C-immune 1.4HBV-Smut tg mice and suppressed (at least transiently) HBV DNA replication49,50. 1.4HBV-Smut tg mice express endogenous HBV-C and circulating HBV-E antigens in the liver48. Antiviral HBV-C-specific CD8+ T-cells are therefore induced under stringent conditions (i.e., operating against the tolerogenic milieu of an antigen expressing liver and the tolerogenic features of the circulating HBV-E in the blood)51 by DNA-based immunization.
In summary, targeting an endogenous RNA adjuvant activity in vaccine recipients by antigens expressing well-defined cationic domains may help to design new generations of DNA vaccines that efficiently prime Th1-biased antibody and T-cell responses against chronic virus infections or tumours52,53.
Materials and Methods
Mice
C57BL/6J (B6) (Janvier, France), TLR-3−/− (Jackson, no. 009675), TLR-7−/− (Jackson, no. 008380) were bred and kept under standard pathogen-free conditions in the animal colony of Ulm University. All mouse immunization studies were carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the German Federal Animal Protection Law. The protocols were approved by the Committee on the Ethics of Animal Experiments of the University of Ulm (Tierforschungszentrum Ulm, Oberberghof) and the Regierungspräsidium Tübingen (Permit Number 992 and 1231 to RS). All immunizations were performed under short time Isofluran anesthesia and all efforts were made to minimize suffering.
Expression of HBV-stC particles in HEK-293 cells
For large scale production of HBV core antigens, we transfected 5 × 108 (80–100 dishes) HEK-293 cells with the indicated plasmids using the calcium phosphate method. HEK-293 cells were used because they can be transfected with high efficacy (≥90%) using the calcium phosphate method and express high levels of vector-encoded antigens (data not shown). 48 hours after transfection, cells were lysed with pH 8.0 lysis buffer [150 mM NaCl, 0.5% NP40 and 100 mM Tris-hydrochloride supplemented Protease Inhibitor Cocktail Tablets (cat. no. 11836145001, Roche Applied Science, Penzberg, Germany)]. Notably, different cell lysis procedures (e.g., CHAPS-detergent containing buffers or detergent-free homogenization)20 can be used in this step that all preserved the particle/RNA complexes. Extracts were cleared by centrifugation and purified using the Strep-tag purification system. Briefly, cell extracts were passed over StrepTactin sepharose (cat. no. 2-1201-025; IBA, Göttingen, Germany) packed polypropylene columns (cat. no. 29922; Pierce, Rockford, USA), purified with five column volumes of wash buffer [150 mM NaCl, 1 mM EDTA and 100 mM Tris–hydrochloride (pH 8.0)] and eluted in eight 500 μl fractions in elution buffer [30 mM NaCl, 0.2 mM EDTA and 20 mM Tris–hydrochloride (pH 8.0) supplemented with 2.5 mM desthiobiotin (cat. no. 2-1000-002; IBA, Göttingen, Germany)]. Where indicated, samples were concentrated in a Speed Vac Concentrator to a volume of 100 μl. Protein samples were analysed on 12.5% SDS-PAGE gels in a Tris-Glycine buffered system and stained with Coomassie blue or on standard 1.2% agarose gels stained with ethidium bromide followed by Coomassie Blue staining of the gels.
Characterization of particle-bound RNA
RNA was extracted from purified particles using RNAmagic reagent (cat. no.: 56-1000-100; Bio-Budget Technologie, Krefeld, Germany) according to the manufacturer’s instructions followed by a further clean-up step with a RNeasy mini kit (cat. no. 74104; Qiagen, Hilden, Germany). RNA samples were directly used for Bioanalyzer (2100 Expert; Agilent Technologies, Inc. Waldbronn, Germany) measurements with the RNA 6000 Nano Kit (cat. no. 5067-1511, Agilent Technologies, Waldbronn, Germany).
Immunization of mice
Mice were immunized intramuscularly (tibialis anterior muscles) with 100 μg plasmid DNA or subcutaneously with indicated amounts of recombinant HBV core antigens in Alhydrogel, kindly provided by Dr. Erik Lindblad (Brenntag Biosector, Frederiksund, Denmark). Gene gun immunization was performed as described in Supplementary Protocols.
Determination of serum antibody titers
Serum samples were obtained by tail bleeding. Murine IgG, IgG1 and IgG2b serum antibody titers were determined by end-point dilution ELISA using bacterial HBV-C149 or -C particles as detection antigens as described previously16. Briefly, microELISA plates (Nunc-Maxisorp, Wiesbaden, Germany) were coated with 150 ng rAgs/well in 50 μl 0.1 M sodium carbonate buffer (pH 9.5) at 4 °C. Serial dilutions of the sera in loading buffer (PBS supplemented with 3% BSA) were added to the Ag-coated wells. Serum Abs were incubated for 2 h at 37 °C followed by fife washes with PBS supplemented with 0.05% Tween 20. Bound serum Abs were detected using HRP-conjugated anti-mouse IgG Abs (catalog no. 554002; BD PharMingen, Hamburg, Germany), IgG1 (catalog no. 559626; BD PharMingen, Hamburg, Germany) and IgG 2b (catalog no. 610-4342; Rockland; Limerick, PA USA) at a dilution of 1/2000 followed by incubation with o-phenylenediamine × 2 HCl in substrate buffer [50 mM Na2HPO4, 25 mM citric acid (pH 5.0)]. The reaction was stopped by 1 M H2SO4 and the extinction was determined at 492 nm. End-point titers were defined as the highest serum dilution that resulted in an absorbance value three times greater than that of negative control sera (derived from non-immunized mice).
Statistics
Data were analyzed using PRISM software (GraphPad, San Diego, CA). The statistical significance of differences in the mean specific antibody titers in sera ±SD and the calculated IgG1:IgG2a ratios ± SD of different groups were determined by the unpaired student’s t-test. P values < 0.05 (*) were considered statistically significant (** significant at p < 0.01, *** significant at p < 0.001).
Data Availability Statement
The data sets generated in this study are available from the corresponding author upon reasonable request.
References
Kutzler, M. A. & Weiner, D. B. DNA vaccines: ready for prime time? Nat Rev Genet 9, 776–788 (2008).
Li, L. & Petrovsky, N. Molecular Adjuvants for DNA Vaccines. Curr Issues Mol Biol 22, 17–40 (2017).
Kurts, C., Robinson, B. W. & Knolle, P. A. Cross-priming in health and disease. Nat. Rev. Immunol 10, 403–414 (2010).
Liang, F. & Lore, K. Local innate immune responses in the vaccine adjuvant-injected muscle. Clin Transl Immunology 5, e74 (2016).
Wu, J. & Chen, Z. J. Innate immune sensing and signaling of cytosolic nucleic acids. Annu. Rev. Immunol 32, 461–488 (2014).
Joffre, O., Nolte, M. A., Sporri, R. & Sousa, R. E. Inflammatory signals in dendritic cell activation and the induction of adaptive immunity. Immunol. Rev 227, 234–247 (2009).
Lau, C. M. et al. RNA-associated autoantigens activate B cells by combined B cell antigen receptor/Toll-like receptor 7 engagement. J. Exp. Med 202, 1171–1177 (2005).
Kelly, K. M. et al. “Endogenous adjuvant” activity of the RNA components of lupus autoantigens Sm/RNP and Ro 60. Arthritis Rheum 54, 1557–1567 (2006).
Green, N. M., Moody, K. S., Debatis, M. & Marshak-Rothstein, A. Activation of autoreactive B cells by endogenous TLR7 and TLR3 RNA ligands. J. Biol. Chem 287, 39789–39799 (2012).
Bernard, J. J. et al. Ultraviolet radiation damages self noncoding RNA and is detected by TLR3. Nat. Med 18, 1286–1290 (2012).
Rock, K. L., Hearn, A., Chen, C. J. & Shi, Y. Natural endogenous adjuvants. Springer Semin. Immunopathol 26, 231–246 (2005).
Dalpke, A. & Helm, M. RNA mediated Toll-like receptor stimulation in health and disease. RNA. Biol 9, 828–842 (2012).
van den Boorn, J. G., Barchet, W. & Hartmann, G. Nucleic acid adjuvants: toward an educated vaccine. Adv Immunol 114, 1–32 (2012).
Birnbaum, F. & Nassal, M. Hepatitis B virus nucleocapsid assembly: primary structure requirements in the core protein. J. Virol 64, 3319–3330 (1990).
Nassal, M. The arginine-rich domain of the hepatitis B virus core protein is required for pregenome encapsidation and productive viral positive-strand DNA synthesis but not for virus assembly. J. Virol 66, 4107–4116 (1992).
Riedl, P. et al. Priming Th1 immunity to viral core particles is facilitated by trace amounts of RNA bound to its arginine-rich domain. J. Immunol 168, 4951–4959 (2002).
Lee, B. O. et al. Interaction of the hepatitis B core antigen and the innate immune system. J. Immunol 182, 6670–6681 (2009).
Tumban, E., Peabody, J., Peabody, D. S. & Chackerian, B. A universal virus-like particle-based vaccine for human papillomavirus: longevity of protection and role of endogenous and exogenous adjuvants. Vaccine 31, 4647–4654 (2013).
Sominskaya, I. et al. A VLP library of C-terminally truncated Hepatitis B core proteins: correlation of RNA encapsidation with a Th1/Th2 switch in the immune responses of mice. PLoS. One 8, e75938 (2013).
Wieland, A. et al. Recombinant complexes of antigen with stress proteins are potent CD8 T-cell-stimulating immunogens. J. Mol. Med 86, 1067–1079 (2008).
Liao, W. & Ou, J. H. Phosphorylation and nuclear localization of the hepatitis B virus core protein: significance of serine in the three repeated SPRRR motifs. J. Virol 69, 1025–1029 (1995).
Selzer, L., Kant, R., Wang, J. C., Bothner, B. & Zlotnick, A. Hepatitis B Virus Core Protein Phosphorylation Sites Affect Capsid Stability and Transient Exposure of the C-terminal Domain. J Biol Chem 290, 28584–28593 (2015).
Ludgate, L. et al. Cell-Free Hepatitis B Virus Capsid Assembly Dependent on the Core Protein C-Terminal Domain and Regulated by Phosphorylation. J Virol 90, 5830–5844 (2016).
Bretscher, P. A. On the mechanism determining the TH1/TH2 phenotype of an immune response, and its pertinence to strategies for the prevention, and treatment, of certain infectious diseases. Scand J Immunol 79, 361–376 (2014).
Bourne, C. R., Katen, S. P., Fulz, M. R., Packianathan, C. & Zlotnick, A. A mutant hepatitis B virus core protein mimics inhibitors of icosahedral capsid self-assembly. Biochemistry 48, 1736–1742 (2009).
Ghebrehiwet, B., Hosszu, K. K., Valentino, A. & Peerschke, E. I. The C1q family of proteins: insights into the emerging non-traditional functions. Front Immunol 3 (2012).
Laine, S. et al. In vitro and in vivo interactions between the hepatitis B virus protein P22 and the cellular protein gC1qR. J. Virol 77, 12875–12880 (2003).
Whitacre, D. C., Lee, B. O. & Milich, D. R. Use of hepadnavirus core proteins as vaccine platforms. Expert. Rev. Vaccines 8, 1565–1573 (2009).
Hirsch, R. C., Lavine, J. E., Chang, L. J., Varmus, H. E. & Ganem, D. Polymerase gene products of hepatitis B viruses are required for genomic RNA packaging as wel as for reverse transcription. Nature 344, 552–555 (1990).
Junker-Niepmann, M., Bartenschlager, R. & Schaller, H. A short cis-acting sequence is required for hepatitis B virus pregenome encapsidation and sufficient for packaging of foreign RNA. EMBO J 9, 3389–3396 (1990).
Knaus, T. & Nassal, M. The encapsidation signal on the hepatitis B virus RNA pregenome forms a stem-loop structure that is critical for its function. Nucleic Acids Res 21, 3967–3975 (1993).
Riedl, P., Buschle, M., Reimann, J. & Schirmbeck, R. Binding immune-stimulating oligonucleotides to cationic peptides from viral core antigen enhances their potency as adjuvants. Eur. J. Immunol 32, 1709–1716 (2002).
Schirmbeck, R., Riedl, P., Zurbriggen, R., Akira, S. & Reimann, J. Antigenic epitopes fused to cationic peptide bound to oligonucleotides facilitate Toll-like receptor 9-dependent, but CD4+ T cell help-independent, priming of CD8+T cells. J. Immunol 171, 5198–5207 (2003).
Yeh, C. T., Wong, S. W., Fung, Y. K. & Ou, J. H. Cell cycle regulation of nuclear localization of hepatitis B virus core protein. Proc. Natl. Acad. Sci. USA 90, 6459–6463 (1993).
Kann, M. & Gerlich, W. H. Effect of core protein phosphorylation by protein kinase C on encapsidation of RNA within core particles of hepatitis B virus. J. Virol 68, 7993–8000 (1994).
Melegari, M., Wolf, S. K. & Schneider, R. J. Hepatitis B virus DNA replication is coordinated by core protein serine phosphorylation and HBx expression. J. Virol 79, 9810–9820 (2005).
Lan, Y. T., Li, J., Liao, W. & Ou, J. Roles of the three major phosphorylation sites of hepatitis B virus core protein in viral replication. Virology 259, 342–348 (1999).
Fotin-Mleczek, M. et al. Messenger RNA-based vaccines with dual activity induce balanced TLR-7 dependent adaptive immune responses and provide antitumor activity. J. Immunother 34, 1–15 (2011).
Heidenreich, R. et al. A novel RNA-based adjuvant combines strong immunostimulatory capacities with a favorable safety profile. Int J Cancer 137, 372–384 (2015).
Brencicova, E. & Diebold, S. S. Nucleic acids and endosomal pattern recognition: how to tell friend from foe? Front Cell Infect. Microbiol 3, 37 (2013).
Pasare, C. & Medzhitov, R. Control of B-cell responses by Toll-like receptors. Nature 438, 364–368 (2005).
Schirmbeck, R. & Reimann, J. Modulation of gene-gun-mediated Th2 immunity to hepatitis B surface antigen by bacterial CpG motifs or IL-12. Intervirology 44, 115–123 (2001).
Schirmbeck, R. & Reimann, J. Revealing the potential of DNA-based vaccination: lessons learned from the hepatitis B virus surface antigen. Biol. Chem 382, 543–552 (2001).
Pumpens, P. & Grens, E. HBV core particles as a carrier for B cell/T cell epitopes. Intervirology 44, 98–114 (2001).
Lin, Y. J. et al. Hepatitis B virus core antigen determines viral persistence in a C57BL/6 mouse model. Proc. Natl. Acad. Sci. USA 107, 9340–9345 (2010).
Yang, P. L. et al. Immune effectors required for hepatitis B virus clearance. Proc. Natl. Acad. Sci. USA 107, 798–802 (2010).
Lin, Y. J., Wu, H. L., Chen, D. S. & Chen, P. J. Hepatitis B virus nucleocapsid but not free core antigen controls viral clearance in mice. J. Virol 86, 9266–9273 (2012).
Halverscheid, L. et al. Transgenic mice replicating hepatitis B virus but lacking expression of the major HBsAg. J. Med. Virol 80, 583–590 (2008).
Riedl, P. et al. Elimination of immunodominant epitopes from multispecific DNA-based vaccines allows induction of CD8 T cells that have a striking antiviral potential. J. Immunol 183, 370–380 (2009).
Riedl, P., Reiser, M., Stifter, K., Krieger, J. & Schirmbeck, R. Differential presentation of endogenous and exogenous hepatitis B surface antigens influences priming of CD8 T cells in an epitope-specific manner. Eur. J. Immunol 44, 1881–1891 (2014).
Bertoletti, A. & Ferrari, C. Adaptive immunity in HBV infection. J Hepatol 64, S71–S83 (2016).
Disis, M. L., Watt, W. C. & Cecil, D. L. Th1 epitope selection for clinically effective cancer vaccines. Oncoimmunology 3, e954971 (2014).
Galaine, J., Borg, C., Godet, Y. & Adotevi, O. Interest of Tumor-Specific CD4 T Helper 1 Cells for Therapeutic Anticancer Vaccine. Vaccines (Basel) 3, 490–502 (2015).
Acknowledgements
We appreciate the expert technical assistance of Ellen Allmendinger and Katrin Schwengle. We thank Dr. Erik Lindblad (Brenntag Biosector, Frederikssund, Denmark) for providing Alhydrogel. This work was supported by grants from the Deutsche Forschungsgemeinschaft (GRK 1789 CEMMA to R.S.) and the International Graduate School in Molecular Medicine Ulm J.K. is a PhD candidate at Ulm University. This work is submitted in partial fulfilment of the requirement for her PhD.
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J.K., K.S., P.R. and R.S. designed the research. J.K., K.S., P.R. performed experiments. J.K. and R.S. wrote the manuscript.
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Krieger, J., Stifter, K., Riedl, P. et al. Cationic domains in particle-forming and assembly-deficient HBV core antigens capture mammalian RNA that stimulates Th1-biased antibody responses by DNA vaccination. Sci Rep 8, 14660 (2018). https://doi.org/10.1038/s41598-018-32971-5
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DOI: https://doi.org/10.1038/s41598-018-32971-5
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