Past fish provenance, exploitation and trade patterns were studied by analyzing phosphate oxygen isotope compositions (δ18OPO4) of gilthead seabream (Sparus aurata) tooth enameloid from archaeological sites across the southern Levant, spanning the entire Holocene. We report the earliest evidence for extensive fish exploitation from the hypersaline Bardawil lagoon on Egypt’s northern Sinai coast, as indicated by distinctively high δ18OPO4 values, which became abundant in the southern Levant, both along the coast and further inland, at least from the Late Bronze Age (3,550–3,200 BP). A period of global, postglacial sea-level stabilization triggered the formation of the Bardawil lagoon, which was intensively exploited and supported a widespread fish trade. This represents the earliest roots of marine proto-aquaculture in Late Holocene coastal domains of the Mediterranean. We demonstrate the potential of large-scale δ18OPO4 analysis of fish teeth to reveal cultural phenomena in antiquity, providing unprecedented insights into past trade patterns.
Fishing was an essential economic component of many ancient societies, as evidenced by the presence of fish remains, fishing gears, and fish-associated artifacts in numerous archaeological sites world-wide1,2,3,4,5. In the southern Levant, past exploitation and trade of fish has been explored primarily based on the occurrences of fish bones in coastal, riverine and lake-side archaeological sites and through inference from the modern distribution patterns, habitat preferences and ecological niches of these fish species. In the Levant, this has mostly been done for fish that a priori were identified as ‘exotic’. For example, the identification of key Nilotic species such as Lates niloticus (Nile perch) and Bagrus sp. (Bagrid catfish) in archeological sites of the southern Levant testified that long-range trade systems between Egypt and Canaan have emerged more than 5000 years ago (during the Early Bronze Age)6,7,8.
The gilthead seabream (Sparus aurata, Linnaeus, 1758) frequently appears in archaeological sites of the southern Levant, since prehistoric times (Late Pleistocene)4,5,6. This species is characterised by thick-enamelled, molar-like teeth (Fig. S1), which are used for cracking shellfish (i.e., bivalves, gastropods and crustaceans)9,10. Sparus aurata is an euryhaline and eurytherm marine fish which migrates between near-shore, inshore (lagoons) and open sea environments11,12,13. Thus, while the appearance of S. aurata in inland sites clearly indicates long range trade systems6,7, remains of this species in Levantine coastal sites have so far been interpreted as reflecting local fishing activity6,7,8.
State of the art research methodologies provide multiple empirical ways to explore trade and maritime connections of desirable fish source marketing to distant places. For example, past provenance and long-range trade of fish from the North Atlantic have been studied using the C and N stable isotopes of bone collagen (Atlantic cod)14,15,16,17,18, and by aDNA analysis18,19. However, fish bone C and N isotope analyses require the preservation of collagen, and they are limited to “young” fish because constant bone remodeling causes the isotopic signature to adjust to local conditions in adult fish14,15. In the North Aegean (northeast Mediterranean), these analyses showed no clustering with locality or species, and for both isotopes they demonstrated a general overlap between freshwater and marine fish, probably due to bone diagenesis20. Hence other isotopic proxies are required to assess fish provenance.
Longinelli and Nuti’s21 pioneering study on the distribution of the phosphate oxygen isotopes (δ18OPO4) in fish bioapatite demonstrated that δ18OPO4 values are controlled by the water temperature and oxygen isotope composition of the ambient water (δ18OWater)22,23. Enameloid of fish teeth is highly resistant to diagenetic alterations24, and thus in many cases preserve information regarding the original salinity and temperature25,26 of their past aquatic habitat (i.e. marine, rivers, lakes, and lagoons)27,28,29,30. In closed or semi-closed water bodies with a high degree of evaporation, the δ18OWater are enriched in 18O relative to the seawater that feeds them23,31 (Fig. S2). Enameloid of fish teeth from such water bodies carry distinctively high δ18OPO4 values, which reflect hypersaline habitats of the fish23,28. The Bardawil lagoon (Sabkhat al Bardawil; along the northern coast of Sinai, Egypt (Fig. 1, 31°09\N, 33°08\E) is such a water body23,31.
This study builds on Sisma-Ventura et al.28, where phosphate oxygen isotopes of tooth enameloid of S. aurata were used as a new proxy to identify the provenance of archaeological fish remains from the Iron Age in the south-east Mediterranean. Our aim is to provide a first long-range assessment of the sources, exploitation and trade of S. aurata in the context of Egypto-Levantine inter-regional interaction and commercialism.
We assess the provenance of ancient S. aurata from archaeological layers in the southern Levant by using phosphate oxygen isotope analysis of fish teeth enameloid as a proxy for fish habitat salinity. We analysed the oxygen isotope ratio (18O/16O in the PO4 group, expressed as δ18OPO4 value; see Methods) of enameloid phosphate of the first molariform teeth (n = 100; Table S1) and jawbones (n = 24, Table S1) of this species from a broad range of 12 coastal and inland archaeological sites spanning the Pre-Pottery Neolithic to the Islamic period: ~9,700 BCE to 600 CE (~11,700–1,400 years BP; Fig. 2).
During this time span, southern Levantine societies evolved from hunting-gathering, to sedentary ways of lives, to complex societies, to territorial states, and were intermittently subsumed under the aegis of vast or lesser empires – from New Kingdom Egyptians in the Late Bronze Age (c. 1,500–1,200 BCE) to the Muslims of the 7th century CE32,33. The fortunes of the Mediterranean southern Levant have always been intertwined with that of its Saharan neighbour in Egypt. Those two different agro-ecological regions were economically interdependent and from the first consolidation of centralized power in Egypt in the 3rd millennium BCE (the Egyptian Old Kingdom), Egypt episodically controlled the southern Levant, whence it could extract the Mediterranean products required for its subsistence and spiritual life. Interconnections between the two regions, however, were not only dictated by cultural and political factors but by climatic fluctuations in both34,35. Traffic between Egypt and the Levant was conducted through marine or terrestrial routes through northern Sinai, which was both affected by it in antiquity and provides archaeological proxies for its intensity today. The main question we asked is whether the distribution of these fish to the Levant was a historically-unique and context-dependant phenomenon or rather of more sustainable nature.
Oxygen Isotopes as Proxy of Fish Aquatic Environment
Bioapatite of fish teeth forms in oxygen isotopic equilibrium with the body fluid at ambient water temperature and δ18OWater21,22,23. Thus, fish record both the environmental temperature and δ18OWater in their bone and tooth δ18OPO4 values25,26,27,28,29,30. While the δ18OWater of the oceans generally shows small variations36,37, closed or semi-closed water bodies, such as coastal lagoons, show significantly elevated and variable δ18OWater values23,31, which are recorded in the hard tissues (i.e. teeth) of migratory fish that exploit these habitats23,28,29,30.
The temperatures of the Eastern Mediterranean littoral generally range from 15 °C in late winter (February–March) to 30 °C in summer (July–August)37. Variations in East Mediterranean δ18OWater are small36,37,38, varying between 1.4‰ (February–March) and 1.8‰ (July–August). Therefore, the calculated δ18OPO4 values (range: 21.2–24.2‰) for bioapatite forming in isotope equilibrium with seawater22 (see Methods) in the southeast Mediterranean reflect mostly the seasonal changes in water temperature (Fig. 3). This agrees well with the δ18OPO4 range from 21.5‰ to 23.4‰ (n = 18) in teeth of modern S. aurata caught in the southeast Mediterranean littoral zone (Fig. 3). In contrast, S. aurata from the Bardawil lagoon along the Southeast Mediterranean (Sinai) coast display significantly higher δ18OPO4 values, between 23.5‰ and 25.4‰23 (Fig. 3). Bardawil lagoon Sparidae are adapted to a salinity value as high as 60‰23. The Bardawil instrumental salinity range typically varies between 36.9‰ and 74.5‰, but could temporarily reach higher values between 70‰ and 90‰, at times when the inlets to the sea were closed artificially39.
The Bardawil lagoon (Fig. 1) is a large (30 km long, 14 km max. width), shallow (0.3–3 m deep) hypersaline coastal lagoon, separated from the Mediterranean Sea by a narrow sandbar39. The Bardawil is connected to the sea via two small natural inlets (Boughaz Zaranik). Water exchange in the lagoon is controlled by Mediterranean Sea tides with a mean height of 50 cm. As a result, it has an elevated salinity level and δ18OWater values around 3.7‰ (range: 1.8‰ near the Mediterranean inlet, reflecting inflowing seawater, up to 7.2‰23,31 (Fig. S2). The unique environmental conditions of the Bardawil lagoon: shallow, warm (17.3–28.3 °C) and hypersaline water (39.0–74.5‰) provides optimal growth conditions for several species of fish, including S. aurata11. Today, juveniles of S. aurata enter the lagoon seeking shelter and food. At about two years of age11,13, most fish reach sexual maturity and leave the nursery, migrating back into the open sea where they live in a variety of habitats such as sea grass beds and sandy or rocky bottoms13,40.
Fish teeth evolve from the epidermal eruption in the skin of the jaw and are continuously replaced throughout the fish’s life cycle41. The δ18OPO4 values in teeth of modern S. aurata cover nearly the entire seasonal range of predicted δ18OPO4values for teeth formed in isotopic equilibrium with the southeast Mediterranean and the Bardawil lagoon water in the according temperature and salinity range, respectively (Fig. 3). We, therefore, assume that tooth formation and replacement occurs on a seasonal basis.
The δ18OPO4 of fish teeth from the archaeological sites in the southern Levant indicate that S. aurata were caught in two distinct habitats: the southeast Mediterranean littoral characterised by low δ18OPO4 values (21.5–23.5‰) and a hypersaline environment reflected by higher δ18OPO4 values (>23.5‰ measured value and >24.2‰ according to the predicted range; see text below for details) (1-Way ANOVA: F = 7.0978, p < 0.001; Table S1, Fig. 4). Thus, it is possible to unambiguously distinguish fish caught from southeast Mediterranean coastal seawater with lower δ18OPO4 values from those caught in hypersaline lagoonal water, characterised by high δ18OPO4 values (Table S1; Fig. 5). However, δ18OPO4 values between 23.5‰ and 24.2‰ can potentially occur in both habitats. Thus, only δ18OPO4 values exceeding 24.2‰ are considered to reflect unambiguously hypersaline habitats.
Notably, records from sediment cores of the Levant coast, dated to the Holocene, show no evidence for any hypersaline lagoon, similar to the Bardawil, neither in the dimension, nor in the unique environmental setting of this habitat42,43. Therefore, as is the case today, the Bardawil lagoon was the only known source for archaeological S. aurata with hypersaline δ18OPO4 values consumed in the Levant. A Bardawil provenance for these specimens from the Late Bronze Age (LBA; ~3,200 BP) to the Byzantine period is further supported by the calculated water temperatures, ranging between 16 and 28 °C (using the mean Bardawil δ18OWater of 3.7‰), which agree well with the annual water-temperature range of the present-day Bardawil lagoon39. We note that the few hypersaline fish dated to the PPN-EBA period (early to mid-Holocene), were likely caught off the southern Levantine shore, and were not exported from the Bardawil, as proposed for the LBA and onward (Late Holocene). This suggests that short-lived, hypersaline lagoons may have formed along the Levant coast when the rapidly rising sea level flooded low-lying coastal areas during the Early Holocene43,44.
Identification of Fishing Grounds/Habitats and Formation of Coastal Lagoons in the Southeast Mediterranean
A key factor in the formation of coastal lagoons in the southeast Mediterranean was the post-glacial stabilisation of the sea level. Over the last 4,000 years sea level stabilised close to its present-day level44,45, reaching the current level (±1m) at around 3,620 ± 160 years BP46 (1,620 BCE; based on optically stimulated luminescence dating of marine sand deposits, overlain by aeolian sand) (Fig. 4A). Sea-level stabilisation allowed the formation of the perennial shallow hypersaline Bardawil lagoon along the northern Sinai coast, due to the establishment of long-shore currents that transported Nile sands which built up blocking sandbars42,43.
During the Early Holocene (Pre-Pottery Neolithic; PPN): PPNA; ~9,700 years BCE; 11,700 years BP) δ18OPO4 values indicate that S. aurata was captured mainly from southeast Mediterranean waters and to a lesser extent from hypersaline lagoons (Fig. 4A). The latter results are the first proof of the past existence and exploitation of hypersaline coastal lagoons along the eastern Mediterranean coast during the Early Holocene. Due to the sharp rise in sea level at the onset of the post-glacial period the nature of these lagoons remains unknown. Nevertheless, local fishing in Mediterranean littoral waters was previously assumed based on fish remains from the now-submerged PPNC site of Atlit-Yam in northern Israel47.
Mid-Holocene (Chalcolithic period and the Early Bronze Age: 4,500–2,500 years BCE; 6,500–4,500 years BP), δ18OPO4 values indicate that S. aurata was primarily captured ‘locally’, namely along the southeast Mediterranean coast (Table S1), however, data are insufficient for reconstructing the variability of fishing grounds for this time span. Currently, we have no data regarding the Middle Bronze Age (MBA). However, by the end of the LBA (~1,200 years BCE; ~3200 years BP), and onwards the fish-harvesting pattern in the southeast Mediterranean changed drastically, shifting to exploitation of S. aurata from a hypersaline source almost exclusively (1-Way ANOVA: F = 7.0978, p < 0.001; Figs 4A and 5). Whereas only 13.9% of the teeth (n = 33) analysed from pre-LBA contexts (PPN to EBA) had δ18OPO4 values indicative of hypersaline habitats, 84.2% of the teeth (n = 57) from the LBA to the Iron Age (IA) were characterised by high δ18OPO4 values typical for fish from hypersaline habitats. This pattern also prevails in the Byzantine period (n = 10). The δ18OPO4 values of sparid teeth from the LBA onwards are similar to those of extant S. aurata from the Bardawil lagoon (Figs 3 and 4A). In addition, from the late LBA onwards, both dentary bone and teeth of the same specimens of S. aurata displayed hypersaline isotopic signatures, suggesting that those fish may have spent their entire life cycle in the Bardawil lagoon (Fig. 6).
The δ18OPO4 values of tooth-jaw pairs of ancient S. aurata specimens from PPN to Byzantine are positively correlated (n = 24, r2 = 0.68), yet δ18OPO4 values of jawbones are consistently lower than those of tooth enameloid. An average offset (Δ18Otooth-jaw) of 1.8 ± 0.95‰ may reflect systematic differences of bioapatite biomineralisation in bones and enameloid. These bone-tooth δ18OPO4 differences suggest that the bone δ18O signatures likely represents a mean of the entire lifespan of S. aurata. This further supports that S. aurata spent a significant part of their adult life in the open sea, but continued to exploit hypersaline coastal lagoons as part of their trophic migration, as is the case with modern S. aurata11,12,13.
Specimens from the Byzantine period have oxygen isotope signatures typical of hypersaline water both in their teeth and jawbone (Fig. 6). This suggests that these fish lived their full life cycle in the hypersaline Bardawil lagoon. Jawbones from other archaeological periods have lower δ18OPO4 values than the teeth from the same individual/jaw, reflecting a marine origin and indicating that these fish lived predominantly in southeast Mediterranean seawater. A few jawbones have values lower than those expected for bioapatite forming in isotope equilibrium with modern southeast Mediterranean seawater. Some alteration of bone, which is known to be more prone to diagenesis than enameloid24, in low δ18O meteoric water during burial may have shifted δ18OPO4 towards lower values.
Fish Body Mass as Indicator for Fishing Intensity
The body size of S. aurata (i.e. total length-TL (cm) and body mass-BM (kg)), can be calculated from the maximum length of the molariform tooth crown48. Body size of S. aurata reflects ontogenetic age and can thus be used as a proxy of fish exploitation (Fig. S3). Smaller average fish body size in both archaeological and modern contexts is associated with higher intensity exploitation of their nursery, i.e. the Bardawil lagoon11,13,29. During the Holocene, the size pattern of S. aurata clearly changes, exhibiting a decrease and lower range in fish size (i.e. harvesting of younger individuals) for specimens from the hypersaline lagoonal waters of the Bardawil (Table S1; Figs 4B and 5). The values estimated for S. aurata body size (body mass and total length) show significant decrease in the range and average size. The range of S. aurata decreased from a mean BM of 1.6 ± 0.8 kg (n = 33) and TL of 45.0 ± 8.3 cm during PPN–Early Bronze Age (EBA) to a mean BM of 1.1 ± 0.4 kg (n = 57) and TL of 40.7 ± 5.1 during LBA–IA, and to a mean of 0.78 ± 0.4 kg (n = 10) and mean TL of 36.4 ± 3.8 in the Byzantine period (Figs 4B and 5), approaching the average fish size of modern aquafarm Sparus (~0.45 kg) (for BM: 1-Way ANOVA: F = 3.9968; p < 0.0001; F = 3.532; For TL: p < 0.001; F = 3.267).
This trend of reduction in fish body size accords chronologically well with cultural changes and spreads over a millennial time-scale, starting in the LBA, continuing to the Iron Age and to the Byzantine period. Interestingly, we witness the appearance of larger fish only at Tel Dor, where we identified in the past S. aurata captured from the Mediterranean littoral zone28. Overall, the reduction in S. aurata mean and maximum body size demonstrates similarity with present day fishery data from the Bardawil lagoon (age group 1–3 years), where S. aurata are intensively exploited due to their abundance in this unique hypersaline nursery11,13. The decrease in S. aurata body size, to a range similar to present-day fish from the lagoon11,13,40 is in line with observations from traditional extensive aquaculture49,50.
From the LBA onwards we find evidence for extensive and likely year-round exploitation of S. aurata in the Bardawil lagoon. This unique exploitation pattern continues until the present10,13, and agrees well with the definition of traditional extensive aquaculture49,50. It is similar, for example, to the Italian ‘vallicoltura’ or the Egyptian ‘hosha’ – both representing traditional fish exploitation systems which utilise natural fish traps by taking advantage of the trophic migration of juveniles from the sea into coastal lagoons. These systems therefore capitalise on naturally occurring foods from this highly productive lagoon11,12,13. Sparus aurata were traditionally exploited extensively in coastal lagoons, until intensive rearing systems for this fish were developed during the 1980s51. Therefore, the cradle of marine aquaculture may be rooted in the Bardawil hypersaline lagoon. For more than 2,000 years it functioned as the major source for Sparus aurata for the Levant. This started not later than the LBA (~1,200 years BCE; 3200 years BP) as extensive harvesting and still continues today as intensive aquafarming11,13.
A Diachronic Summary: Fish Exploitation in The Context of Egypto-Levantine Exchanges
In the southern Levant, for the last 50,000 years, S. aurata were exploited by local coastal fishing communities4,5. The first evidence for fishing in hypersaline lagoons appears during the PPNB ca. 9,500 years BP, but these remains are scarce and are insufficient to indicate systematic exploitation of hypersaline lagoons during this period. However, the occurrence of fish remains in Neolithic inland sites of the Judean Mountains (central Israel) indicates that the transportation of dry fish from the Mediterranean coast was already established in the Early Holocene4,52.
From the LBA period onwards, non-local (“exotic”) S. aurata with hypersaline δ18OPO4 signatures were imported from the Bardawil lagoon, almost entirely replacing S. aurata caught locally in coastal waters. These results contradict the conventional null hypothesis assuming that in coastal sites of the Levant S. aurata remains will represent local fishing, exhibiting that regardless of the site location (coastal or inland) most of the S. aurata were “exotic” (nonlocal) with δ18OPO4 signatures of the Bardawil lagoon.
In archaeological sites across the southern Levant, this fundamental change in fish provenance coincides with a sharp increase in the abundance of exotic Nilotic fish, such as Nile perch (Lates niloticus) and the Nile catfish6,7 (Table S3). Our results, therefore, provide new evidence for the intensity of Egypto-Canaanite long-distance, inter-regional trade connections in this period, which seem to have even included commercialization of fish from Bardawil lagoon. This pattern, which further intensified in the Iron Age and lasted at least until the Byzantine period, most likely comprised dried S. aurata, as depicted in Egyptian reliefs53 and as observed from the fragmentation patterns on some of the S. aurata remains recovered in Jerusalem (inland)54.
Contextualising the Results Historically
Prior to Classical times, the densest network of archaeological sites in northern Sinai is documented for the LBA, the imperial epoch of Egypt’s New Kingdom. In this extremely arid, inhospitable region, meaningful population and economic infrastructure could only be sustained when backed by a centralised power. During the LBA Egypt controlled Canaan55 and well-documented terrestrial and maritime routes across northern Sinai and along the coast served as the main military and commercial artery between the two regions. Intensive traffic between them is recorded both textually and archaeologically, the latter including dozens of waystations, granaries, reservoirs, etc.56,57. The Egyptians called this route The Ways of Horus—the southernmost leg of the famed international trunk road linking Egypt with the Fertile Crescent, better known today as the Via Maris.
It is easy to envisage how the Bardawil fish industry and export emerged and functioned in this context. Although our earliest substantial evidence of S. aurata teeth with Bardawil-like, hypersaline δ18OPO4 signatures in Canaan dates to the early 12th century BCE (at Lachish, an Egyptian administrative centre), the single 14th century BCE hypersaline specimen found at Tel Rehov may hint at an earlier beginning of this trade during the LBA. Moreover, although we lack data from the MBA (~2,000–1,500 BCE), it is possible that this phenomenon started even earlier. Throughout the MBA, contact between Canaan and Egypt was close, although at the end of this period administrative control was inversed: Canaanites controlled parts of Egypt at this time. Egypto-Canaanite commerce flourished in this period too, though most of it appears to have been conducted via the sea56. Dozens of MBA settlements have been surveyed in northern Sinai, especially south of the Bardawil lagoon.
Importantly, our findings demonstrate that despite climatic changes and frequent socio-political, economic and demographic upheavals in both regions, once industry and marketing were in motion, they lasted at least until Byzantine times (i.e. minimally for two millennia), providing a paradigmatic example of a Mediterranean exchange network driven by the diversity and interdependence between ecological micro-regions58,59.
As if to support our main claim in this paper, Rabbi Abbahu, a Jewish sage living in 4th-century CE Caesarea Maritima—a major Southeast Mediterranean harbour city (just south of Tel Taninim, Fig. 2)—declared that: “any fish [brought to the city] must come either from Apamea [in Syria] or from Pelusium [Bardawil’s harbour town from the 6th century BCE until the drying up of the eastern arm of the Nile60]”. Our results support Safrai’s concomitant assumption that even in coastal markets most of the fish in Roman Palestine were imported and demonstrate that this state of affairs had already been in place many centuries earlier.
A sample of 100 S. aurata molariform teeth recovered from 12 archaeological sites in the southern Levant was analysed in this study. Identification to species level is based on modern Mediterranean ichthyofauna housed at the University of Haifa reference collection and on OL’s personal research collection. Modern S. aurata teeth for this study were obtained from specimens captured offshore in Haifa Bay, Israel28. Their phosphate oxygen isotope signatures as well as those of extant S. aurata from the Bardawil lagoon23 were compared with those of teeth from archaeological S. aurata to assess their past habitats, specifically whether they derive from southeast Mediterranean marine or hypersaline lagoonal waters.
The enamel cap (~0.2–0.4 mm thickness) of each tooth was separated from the dentine using a diamond-head micro-dental drill, washed three times with distilled water, and dried overnight at 50 °C. Each sample was crushed and ground to powder using an agate mortar and pestle. Organic matter was removed from the samples soaking the samples in 2% NaOCl overnight. The phosphate fraction of the samples was separated using a method modified after Dettmann et al.61 and described in detail by Gehler et al.62. In summary, approximately 5 mg of pretreated sample powder was dissolved in 0.8 ml 2 M HF and placed on a vibrating table for ca. 12 h. After centrifuging, the supernatant sample solution was separated from the CaF2 precipitate and transferred to new centrifuge tubes. After neutralising the HF solution with NH4OH (25%) in the presence of bromothymol blue as a pH indicator, Ag3PO4 was rapidly precipitated by adding 0.8 ml of 2 M AgNO3. Following settling of the Ag3PO4 crystals, the samples were centrifuged and the supernatant solution was removed using a pipette. The Ag3PO4 was then rinsed five times with MilliQ water and dried overnight in an oven at 50 °C.
Ag3PO4 aliquots of 0.5 mg were placed into silver capsules and analysed in triplicate by means of high temperature reduction using a Finnigan TC-EA coupled via a Conflo III to a Micromass 100 GC-IRMS at the University of Mainz, or to a Finnigan Delta Plus XL GC-IRMS at the Universities of Tübingen and Lausanne, following Vennemann et al.63. Measured 18O/16O isotope ratios are reported in the δ-notation:
i.e., as the deviation in per mil (‰) relative to Vienna Standard Mean Ocean Water (VSMOW), the international reference material. The δ18OPO4 values were measured with an external precision of ±0.3‰ (1 SD).
The raw δ18OPO4 values were normalised to an Ag3PO4 standard produced by Elemental Microanalysis with a certified value of 21.7‰ (silver phosphate P/N IVA33802207, batch no. 180097, distributed by IVA Analysentechnik, Germany). The analytical precision for this standard was better than ±0.3‰ (1σ). For untreated NIST SRM 120c Florida phosphate rock standard reference material, we obtained a δ18OPO4 value of 21.9 ± 0.3‰ (n = 9). This value compares well with the values around 21.7‰ initially measured by Lécuyer et al.64 and currently reported by most other laboratories as compiled in Chenery et al.65.
The δ18OPO4 theoretical range of Sparidae bioapatite
We calculated the equilibrium range of δ18OPO4 in the littoral of the southeast Mediterranean and in hypersaline lagoons, evolving from typical southeast Mediterranean water. The calculation is based on the temperature-dependent relation for isotope fractionation during biomineralisation of apatite by Lécuyer et al.22: T °C = 117.4–4.5(δ18OPO4 − δ18OSeaWater), where δ18OPO4 and δ18OSeaWater correspond to the isotope compositions of bio-apatite and seawater relative to VSMOW, respectively. This relation is valid for the temperature range of 8 °C < T < 32 °С relevant for the water temperatures encountered in the Mediterranean realm37,38.
Body mass estimation of S. aurata
Body mass of ancient fish can be estimated from species-specific regressions with bone or molariform tooth size. In this study, we used the tooth length measurements recommended for the first molariform tooth (Fig. S1), and regression equations to estimated fish total length (cm) and body mass (kg)48:
Linear regression calculated from S. aurata first molariform tooth maximum length (FMTL) to fish total length (TL).
 TL = 32.21*FMTL + 154.07 (r2 = 0.83; Fig. S3)
Linear regression demonstrating the correlation between TL to fish body mass (FBM)
 FBM = 1.7086*e−05*TL2.977 (r2 = 0.98).
O’Connor, S., Ono, R. & Clarkson, C. Pelagic fishing at 42,000 years before the present and the maritime skills of modern humans. Science 334, 1117–1121 (2011).
Marean, C. W. et al. Early human use of marine resources and pigment in South Africa during the Middle Pleistocene. Nature 449, 905–909 (2007).
Stewart, K. M. Fishing Sites of North and East Africa in the Late Pleistocene and Holocene. (British Archaeological Reports International Series, 521, Oxford, UK, 1989).
Bar-Yosef Mayer, D. E. & Zohar, I. The role of aquatic resources in the Natufian culture. Eurasian Prehistory 7, 31–45 (2010).
Zohar, I. Fish exploitation during the Quaternary: Recent knowledge, In: Enzel, Y. & Bar-Yosef, O. (Eds), Quaternary of the Levant: Environments, Climate Change, and Humans. (Cambridge University Press, University Printing House, Cambridge, United Kingdom, pp. 369–376, 2017).
Van Neer, W. et al. Fish remains from archaeological sites as indicators of former trade connections in the Eastern Mediterranean. Paléorient 30, 101–148 (2004).
Van Neer, W., Zohar, I. & Lernau, O. The emergence of fishing communities in the eastern Mediterranean region: A survey of evidence from pre- and protohistoric periods. Paléorient 31, 131–157 (2005).
Raban-Gerstel, N., Bar-Oz, G., Zohar, I., Sharon, I. & Gilboa, A. Early Iron Age Dor (Israel): A faunal perspective. Bulletin of the American School of Oriental Research 349, 25–59 (2008).
Tancioni, L. et al. Locality-specific variation in the feeding of Sparus aurata: Evidence from two Mediterranean lagoon systems. Estuar. Coast. Shelf Sci. 57, 469–474 (2003).
Pita, C., Gamito, S. & Erzini, K. Feeding habits of the gilthead seabream (Sparus aurata) from the Ria Formosa (southern Portugal) as compared to the black seabream (Spondyliosoma cantharus) and the annular seabream (Diplodus annularis). J. Appl. Ichthyol. 18, 81–86 (2002).
Ahmed, S. M. Population dynamics and fisheries management of gilthead sea bream, Sparus aurata (Sparidae) from Bardawil lagoon, North Sinai, Egypt. Egypt J. Aquat. Biol. Fish 15, 57–69 (2011).
Chaoui, L., Kara, M. H., Faure, E. & Quignard, J. P. Growth and reproduction of the gilthead seabream Sparus aurata in Mellah lagoon (north-eastern Algeria). Sci. Mar. 70, 545–552 (2006).
Ben-Tuvia, A. Biological basis for the fishery regulation and management of the Bardawil Lagoon, Mediterranean coast of Sinai. (FAO, Rome, Italy, 1985).
Barrett, J. et al. Detecting the medieval cod trade: a new method and first results. J. Archaeol. Sci. 35, 850–861 (2008).
Barrett, J. et al. Interpreting the expansion of sea fishing in medieval Europe using stable isotope analysis of archaeological cod bones. J. Archaeol. Sci. 38, 1516–1524 (2011).
Orton, D. C. et al. Stable isotope evidence for late medieval (14th–15th C) origins of the eastern Baltic Cod (Gadus morhua) fishery. PLoS ONE 6, e27568 (2011).
Orton, D. C., Morris, J., Locker, A. & Barrett, J. H. Fish for the city: Meta-analysis of archaeological cod remains as a tool for understanding the growth of London’s northern trade. Antiquity 88, 516–530 (2014).
Star, B. et al. Ancient DNA reveals the Arctic origin of Viking Age cod from Haithabu, Germany. Pnas 114(34), 9152–9157 (2017).
Arndt, A. et al. Roman trade relationship at Sagalassos (Turkey) elucidated by ancient DNA of fish remains. J. Archaeol. Sci. 30, 1095–1105 (2003).
Vika, E. & Theodoropoulou, T. Re-investigating fish consumption in Greek antiquity: results from δ13C and δ15N analysis from fish bone collagen. J. Archaeol. Sci. 39, 1618–1627 (2012).
Longinelli, A. & Nuti, S. Revised phosphate–water isotopic temperature scale. Earth Planet. Sci. Lett. 19, 373–376 (1973).
Lécuyer, C., Amiot, R., Touzeau, A. & Trotter, J. Calibration of the phosphate δ18O thermometer with carbonate–water oxygen isotope fractionation equations. Chem. Geol. 347, 217–226 (2013).
Kolodny, Y., Luz, B. & Navon, O. Oxygen isotope variations in phosphate of biogenic apatites, I. Fish bone apatite – rechecking the rules of the game. Earth Planet. Sci. Lett. 64, 398–404 (1983).
Sharp, Z. D., Atudorei, V. & Furrer, H. 2000. The effect of diagenesis on oxygen isotope ratios of biogenic phosphates. Am. J. Sci. 300, 222–237 (2000).
Pucéat, E. et al. Thermal evolution of Cretaceous Tethyan marine waters inferred from oxygen isotope composition of fish tooth enamels. Paleoceanography 18, 1–11 (2003).
Kolodny, Y. & Raab, M. Oxygen isotopes in phosphatic fish remains from Israel: paleothermometry of tropical Cretaceous and Tertiary shelf waters. Palaeogeogr. Palaeoclimatol. Palaeoecol. 64, 59–67 (1988).
Dufour, E. et al. Oxygen and strontium isotopes as provenance indicators of fish at archaeological sites: the case study of Sagalassos, SW Turkey. J. Archaeol. Sci. 34, 1226–1239 (2007).
Sisma-Ventura, G. et al. Oxygen isotope composition of Sparidae (sea bream) tooth enamel from well-dated archaeological sites as an environmental proxy in the East Mediterranean: A case study from Tel Dor, Israel. J. Archaeol. Sci. 64, 46–53 (2015).
Otero, O. et al. Freshwater fish δ18O indicates a Messinian change of the precipitation regime in Central Africa. Geology 39, 435–438 (2011).
Fischer, J. et al. Oxygen and strontium isotopes from fossil shark teeth: environmental and ecological implications for Late Palaeozoic European basins. Chem. Geol. 342, 44–62 (2013).
Aly, A. I. M., Hamed, M. A., Abd El-Samie, S. G. & Eweida, E.A. Environmental isotopes and hydrochemistry approach to evaluate the source of recharge and pollution load in Manzala and Bardawil Lakes, Egypt (IAEA-CN-118/85). Isotopes in Environmental Studies Aquatic Forum (Conference and Symposium Papers 26/P, 172–173, 2004).
Levy, E. T. The archaeology of society in the Holy Land (London, Continuum, 2003).
Killebrew, A. E. & Steiner, M. (eds). Oxford Handbook of the Archaeology of the Levant (ca. 8000–332 BCE) (Oxford University Press, 2013).
Bernhardt, C. E., Benjamin, P., Horton, B. P. & Stanley, J. D. Nile delta vegetation response to Holocene climate variability. Geology 40, 615–618 (2012).
Langgut, D. et al. Dead Sea Pollen Record and History of Human Activity in the Judean Highlands (Israel) from the Intermediate Bronze into the Iron Ages (~2500–500 BCE). Palynlogy 38(2), 280–302 (2014).
Pierre, C. The oxygen and carbon isotope distribution in the Mediterranean water masses. Mar. Geol. 153, 41–55 (1999).
Sisma-Ventura, G., Yam, R., Kress, N. & Shemesh, A. Water column distribution of oxygen and carbon isotopes in the Levantine basin: temporal and vertical change. J. Marine System 158, 13–25 (2016).
Sisma-Ventura, G., Yam, R. & Shemesh, A. Recent unprecedented warming and oligothrophy of the Eastern Mediterranean Sea within the last millennium. Geophys. Res. Lett. 41, 5158–5166 (2014).
Khalil, M. T., Saad Abd E., H. A., Fishar, M. R. & Bedir, T. Z. Ecological Studies on Macrobenthic Invertebrates of Bardawil Wetland, Egypt. World Environment 3, 1–8 (2013).
Mehanna, S. F. A Preliminary Assessment and Management of Gilthead Bream Sparus aurata in the Port Said Fishery, the Southeastern Mediterranean, Egypt. Turk. J. Fish. Aquat. Sci. 7, 123–130 (2007).
Elgendy, S. A., Alsafy, M. A. & Tanekhy, M. Morphological characterization of the oral cavity of the gilthead seabream (Sparus aurata) with emphasis on the teeth-age adaptation. Microscopy research and technique 79, 227–236 (2016).
Sivan, D., Greenbaum, N., Cohen-Seffer, R., Sisma-Ventura, G. & Almogi-Labin, A. The origin and disappearance of the Late Pleistocene – Early Holocene short-lived coastal wetlands along the Carmel coast, Israel. Quat. Res. 76, 83–92 (2011).
Elyashiv, H. et al. The interplay between relative sea-level rise and sediment supply at the distal part of the Nile littoral cell. The Holocene 26, 248–264 (2016).
Sivan, D., Wdowinski, S., Lambeck, K., Galili, E. & Raban, A. Holocene sea-level changes along the Mediterranean coast of Israel, based on archaeological observations and numerical model. Palaeogeogr. Palaeoclimatol. Palaeoecol. 167, 101–117 (2001).
Lambeck, K. & Chappell, J. Sea level change through the last glacial cycle. Science 292, 679–686 (2001).
Porat, N., Sivan, D. & Zviely, D. Late Holocene embayment and sedimentological infill processes in Haifa Bay, SE Mediterranean. Israel J. Earth Sci. 57, 21–23 (2008).
Galili, E., Zviely, D. & Weinstein-Evron, M. Holocene sea-level changes and landscape evolution in the northern Carmel coast (Israel). Méditerranée 104, 79–86 (2005).
Desse, J. & Desse-Berset, N. Ostéométrie et archéologie de la daurade royale (Sparus aurata, Linné 1758). Fiches d’ostéologie animale pour l’archéologie. Série A, Poissons, APDCA, 36 p (1996).
Beveridge, M. C. M. & Little, D. C. The History of Aquaculture in Traditional Societies, In: Costa-Pierce, B.A. (Ed.), Ecological Aquaculture: The Evolution of the Blue Revolution, (Blackwell Science Ltd, Oxford, UK, 2007).
Marzano, A. Harvesting the Sea: The exploitation of marine resources in Roman Mediterranean (Oxford University Press, Oxford, UK, 2013).
Moretti, A., Pedini Fernandez-Criado, M. & Vetillart, R. Manual on hatchery production of seabass and gilthead seabream (FAO, Rome, Italy, 2005).
Davis S. J. M. A preliminary report of fauna from Hatula: a Natufian-Khiamian (PPNA) site near Latroun, Israel, in: Lechevallier, M., Ronen, A. (Eds), Le site Natufian-Khiamien de Hatoula, pres de Latroun, Israel., (Centré de Rechérché Français de Jerusalem, Jerusalem, Israel, 1985).
Brewer, D. J. & Friedman, R. F. Fish and fishing in ancient Egypt (The American University in Cairo Press, Cairo, Egypt, 1990).
Horwitz, L. K. & Lernau, O. Iron Age IIb faunal remains from the Ophel, Area A2009, In: Mazar, E. (Ed.), The Ophel Excavations to the South of the Temple Mount 2009–2013, Shoam Academic Research Publication, Israel, 289–309 (2018).
Killebrew, E. A. Biblical Peoples and Ethnicity: An Archaeological Study of Egyptians, Canaanites, Philistines, and Early Israel 1300‒1100 B.C.E. (Society of Biblical literature, Atlanta, USA, 2005).
Oren, D. E. The Establishment of Egyptian Imperial Administration on the ‘Ways of Horus’: An Archaeological Perspective from North Sinai, Ernst Czerny et al. (eds), Timelines: Studies in Honour of Manfred Bietak, vol. 2 (Orientalia Lovaniensia analecta 149), Leuven: Uitgeverij Peeters en Departement Oosterse Studies, 2006).
Hoffmeier, K. J. & Moshier, O. S. Desert Road Archaeology in Ancient Egypt and Beyond (Heinrich-Barth-Institut, Köln, Germany, 2013).
Marcus, E. Venice on the Nile? On the Maritime Character of Tell Dab’a/Avaris. In Timelines: Studies in Honour of Manfred Bietak, edited by E. Czerny, I. Hein, H. Hunger, D. Melman, and A. Schwab. (Peeters, Leuven and Paris, 2006).
Horden, P. & Purcell, N. The Corrupting Sea: A Study of Mediterranean History. (Blackwell, Oxford, UK, 2000).
Safrai, Z. The Economy of Roman Palestine. (Routledge, London, UK, 1994).
Dettmann, D. L. et al. Seasonal stable isotope evidence for a strong Asian monsoon throughout the past 10.7 m.y. Geology 29, 31–34 (2001).
Gehler, A., Tütken, T. & Pack, A. Oxygen and Carbon Isotope Variations in a Modern Rodent Community – Implications for Palaeoenvironmental Reconstructions. PLoS ONE 7(11), e49531 (2012).
Vennemann, T. W., Fricke, H. C., Blake, R. E., O’Neil, J. R. & Colman, A. Oxygen isotope analysis of phosphates: a comparison of techniques for analysis of Ag3PO4. Chem. Geol. 185, 321–336 (2002).
Lécuyer, C., Grandjean, P., O’Neil, J. R., Cappetta, H. & Martineau, F. Thermal excursions in the ocean at the Cretaceous–Tertiary boundary (northern Morocco): δ18O record of phosphatic fish debris. Palaeogeography, Palaeoclimatology, Palaeoecology 105, 235–243 (1993).
Chenery, C., Muldner, G., Evans, J., Eckardt, H. & Lewis, M. Strontium and stable isotope evidence for diet and mobility in Roman Gloucester, UK. J. Archaeol. Sci. 37, 150–163 (2010).
This study was financially supported by the German Research Council (AP: grant PA 909/15-1), the Georg-August-University of Göttingen, and by the European Research Council under the European Union’s Horizon 2020 Research and Innovation program (grant agreements # 648427 (to GBO) and # 681450 (to TT)) and the Israel Science Foundation (grant # 340-14). We thank Michael Maus (Applied and Analytical Palaeontology Group, Johannes Gutenberg University of Mainz), Bernd Steinhilber (Geochemistry Group, University of Tübingen) and Torsten Vennemann (University of Lausanne) for their assistance with δ18OPO4 analyses. We thank the following archaeologists for allowing us to study the fish from their excavations: Eliot Braun, Tali Erickson-Gini, Yossi Garfinkel, Sy Gitin, Amir Golany, Thomas E. Levy, Daniel Master, Amihai Mazar, Ronny Reich, Avraham Ronen, Ilan Sharon, Rafi Stieglitz, Nahshon Szanton, Yotam Tepper, David Ussishkin, Joe Uziel. IZ thanks the Irene Levi Sala CARE archaeological foundation for supporting the establishment of her fish reference collection.
The authors declare no competing interests.
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Guy, SV., Thomas, T., Irit, Z. et al. Tooth oxygen isotopes reveal Late Bronze Age origin of Mediterranean fish aquaculture and trade. Sci Rep 8, 14086 (2018). https://doi.org/10.1038/s41598-018-32468-1
- Bardawil Lagoon
- Phosphate Oxygen Isotope
- Tooth Enameloid
- Fish Teeth
- Northern Sinai
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