Therapy of Burkholderia pseudomallei acute infections is largely limited to a few β-lactam antibiotics such as ceftazidime or meropenem. Although relatively rare, resistance emergence during therapy leads to treatment failures with high mortality rates. In the absence of acquired external resistance determinants in B. pseudomallei emergence of β-lactam resistance is invariably caused by mutational modification of genomically encoded factors. These include the deletion of the ceftazidime target penicillin-binding protein 3 or amino acid changes in the Class A PenA β-lactamase that expand its substrate spectrum, as well as penA gene duplication and amplification or its overexpression via transcriptional up-regulation. Evidence is presented that penA is co-transcribed with the upstream nlpD1 gene, that the transcriptional terminator for nlpD1 serves as a penA attenuator and that generation of a new promoter immediately upstream of the terminator/attenuator by a conserved G to A transition leads to anti-termination and thus constitutive PenA expression and extended β-lactam resistance. Further evidence obtained with the extensively β-lactam resistant clinical isolate Bp1651 shows that in addition to PenA overexpression and structural mutations other adaptive mechanisms contribute to intrinsic and acquired B. pseudomallei β-lactam resistance.
Burkholderia pseudomallei is an opportunistic pathogen prevalent in tropical and sub-tropical regions around the world where it causes melioidosis, a multifacteted disease syndrome1,2. B. pseudomallei infections are difficult to treat because of the bacterium’s intrinsic antibiotic resistance3,4. Although not yet well studied in B. pseudomallei, this intrinsic resistance is likely complex and due to an impermeable outer membrane, expression of endogenous resistance factors such as efflux pumps and β-lactamase, as well as biofilm and intracellular lifestyles5,6,7,8. Unlike most other Gram-negative bacteria, acquired resistance due to horizontal gene transfer has not yet been documented in B. pseudomallei, but rather is due to expression of chromosomally-encoded resistance determinants. Because β-lactam antibiotics are crucial for acute phase melioidosis therapy, acquired resistance to these antibiotics has been studied in some detail. B. pseudomallei is intrinsically resistant to many β-lactam antibiotics such as amoxicillin and carbenicillin due to expression of chromosomally-encoded PenA β-lactamase9. In combination with clavulanic acid, amoxicillin has clinical utility4. Because PenA exhibits only weak cephalosporinase activity, and even weaker carbapenemase activity, ceftazidime (CAZ) and meropenem (MEM) are drugs of choice for treatment of acute B. pseudomallei infections4. However, acquired CAZ resistance (CAZr) by up-regulation of penA due to a putative promoter mutation10,11,12, penA gene duplication and amplification13, and PenA amino acid substitutions that extend the enzyme’s substrate spectrum10,12,14,15,16 have all been shown to occur in clinical isolates as the result of CAZ therapy. Although PenA is the prominent player in B. pseudomallei’s CAZr, other mechanisms do likely exist but only CAZr due to penicillin-binding protein 3 target deletion has been shown17. We previously described a strain of Australian origin, Bp1651, that exhibited a multidrug resistant phenotype, including resistance to CAZ and imipenem (IPM), and reduced susceptibility to MEM12. The data were consistent with the hypothesis that β-lactam resistance in this strain is multifactorial, with penA likely being the dominant player, e.g. via synergy between penA up-regulation and novel PenA structural mutations that result in high level CAZ and IPM resistance12. Synergy leading to CAZr has been observed before in other clinical isolates10. In this study, we build on previous observations and examine the chromosomal organization of penA and dominant regulatory features that affect its expression in and contribution to β-lactam resistance of Bp1651 by comparison with the CAZ susceptible (CAZs) prototype strain 1026b.
Organization of the penA chromosomal region
In a quest to understand the transcriptional organization of the penA region on chromosome 2 we first examined its surrounding sequences. The penA gene is separated from the upstream nlpD1 gene by 174 bp (Fig. 1a). NlpD1 is annotated as an outer membrane lipoprotein and contains conserved motifs present in E. coli NlpD, one of two activators of cell wall hydrolytic amidase activity18. This organization is conserved in closely related members of the B. pseudomallei complex, e.g. B. mallei and B. thailandensis (Fig. S1). Bioinformatic analyses did not identify any promoter sequences in the nlpD1-penA intergenic region (IR) or within nlpD1, suggesting that the two genes may form an operon transcribed from a promoter located upstream of nlpD1. The sequences of the IR of diverse B. pseudomallei strains are highly similar, with few conserved single nucleotide changes and a rare insertion (Fig. 1b). Surprisingly, in addition to a potential palindrome constituting a possible transcriptional terminator downstream of penA, we identified a 49 nucleotide palindromic sequence capable of forming a stem-loop structure in the penA 5′-untranslated region (5′-UTR) in the nlpD1-penA IR. In the Burkholderia Genome Database (www.burkholderia.com) 1026b entry this sequence was recently annotated as Rho-independent transcription terminator 264 (TERM 264) (Fig. 2). The average free energy reported for E. coli terminators is −10 kcal/mol19. By comparison, the calculated free energy for TERM 264 is high (∆G = −35.7 kcal/mol), which is comparable to that of the strong E. coli rRNA operon rrnB T1 Rho-independent terminator (−32.2 kcal/mol; calculated with the same software used in the present study)20. These observations laid the basis for comparative studies with the previously identified extensively β-lactam resistant strain Bp1651 and the CAZs strain 1026b that explore regulatory features affecting penA expression and contribution to β-lactam resistance.
The nlpD1 and penA genes form an operon
To assess potential polycistronic transcription of nlpD1 and penA, we performed RT-PCR with primers designed to cover different portions of the nlpD1-penA region (Fig. 3a). Utilizing cDNA derived from 1026b total RNA as template, primer pair 2999 & 3003 amplified a 904 bp fragment consisting of 336 bp of the 3′ portion of nlpD1, the 174 bp nlpD1-penA IR and 394 bp of the 5′ portion of penA (Fig. 3b, middle panel, lane 1). The presence of this fragment indicated that the two genes are co-transcribed, located on the same polycistronic mRNA and thus likely belong to the same operon. Fragment intensity is significantly less with cDNA derived from Bp1651 total RNA as template (Fig. 3b, middle panel, lane 2), likely because the main penA transcript in this strain originates from a promoter, hereafter referred to as PA, that arises from a G to A transition at position −78 in the IR (Figs 1b and 2b), so the cDNA derived from this transcript does not contain the P2999-binding site. The intensity of the RT band from the Bp1651 cDNA is similar to that observed with nlpD1 cDNA (Fig. 3b, left panel) indicating that the transcripts are derived from the same promoter upstream of nlpD1 and not very abundant. The 1026b nlpD1-penA IR fragment cDNA intensity is significantly higher than that observed from the 1026b cDNA nlpD1 fragment (compare Fig. 3b, left and middle panel, lanes 1). The penA cDNA band intensities obtained with both 1026b and Bp1651 RNA-derived templates (Fig. 3b, right panel, lanes 1 and 2) are significantly higher when compared to those observed with nlpD1 and nlpD1-penA (Fig. 3b, left and middle panel). cDNA levels of penA obtained with RNA from Bp1651 (Fig. 3b, right panel lane 2) were higher than those observed in 1026b (Fig. 3b, right panel, lane 1).
RT-qPCR analyses confirmed that penA transcript levels are >8-fold higher in Bp1651 when compared to 1026b (Fig. 3c), which is consistent with increased penA expression due to the presence of the PA promoter. The data also confirm that nlpD1 transcript levels are significantly lower than penA transcript levels in both strains.
A locally conserved single mutation generates a penA promoter
Up-regulated penA expression was shown as a cause of CAZr over 25 years ago9. Several studies subsequently demonstrated the presence of a conserved G to A transition at position −78 upstream of the penA start codon (Figs 1b and 2b) and it was suggested that the mutation likely generates a functional −10 region that is absent in wild-type strains10,12. However, until now little experimental evidence supported these notions.
The penA upstream regions of the CAZ susceptible (CAZs) strain 1026b and the CAZr strain Bp1651 differ by point mutations at positions −171, −78, and −41 upstream of the penA initiation codon (Fig. 4a). To assess the possible contributions of these point mutations to penA expression they were individually introduced into the 1026b upstream sequence. Transcriptional penA’-lacZ fusions were then generated by cloning fragments containing the 1026b, the Bp1651 IR and the 1026b IR with the engineered Bp1651 −171C, −78A and −41T mutations into a mini-Tn7-lacZ fusion vector. The mini-Tn7-penA’-lacZ fusion containing elements were transposed into the Bp82.27 genome, along with the empty vector and β-Galactosidase (β-Gal) activity was measured (Fig. 4b). Low levels of β-Gal activity were expressed in strains harboring the empty vector (Bp82.363) or penA’-lacZ reporter constructs with the 1026b (Bp82.364 and Bp82.372; short and long, respectively), −41T (Bp82.366) and −171C (Bp.82.371) containing penA upstream regions. In contrast, high levels of β-Gal activity were expressed in strains harboring penA’-lacZ reporter constructs with the Bp1651 (Bp82.365) and 1026b with −78A (Bp82.367) containing penA upstream regions, indicating that the −78A mutation generates a promoter (PA) that leads to constitutive overexpression of penA.
PA is active in E. coli since strain DH5α carrying a plasmid with a penA’-lacZ translational fusion containing the 174 bp 1026b IR with the −78A mutation and the first seven amino acids of PenA fused in-frame to LacZ expressed β-Gal activity (Fig. 4c).
The transcriptional terminator located upstream of penA is functional
To assess whether the predicted Rho-independent TERM 264 in the penA 5′-UTR functions as transcriptional terminator, constructs were engineered where the penA’-lacZ fusion is expressed from the constitutive B. thailandensis S12 promoter (PS12) with and without TERM 264 (Fig. 5a). The mini-Tn7-penA’-lacZ fusion containing elements were transposed into the Bp82.27 genome, along with the empty mini-Tn7-lacZ vector and β-Gal activity was measured (Fig. 5b). Placement of PS12 134 bp upstream of TERM 264 (Bp82.374) completely abolished expression of β-Gal activity to levels that are below those observed with the empty vector control (Bp82.363). In contrast, placement in the same position in a construct with a clean TERM 264 deletion (Bp82.375) resulted in high levels of β-Gal activity. Bp82.380 containing PS12 24 bp upstream of TER M264 expressed high levels of β-Gal activity and this activity is slightly increased in Bp82.381 where PS12 is placed in the same position in a construct lacking TERM 264.
β-Lactam resistance in Bp1651 extends beyond PenA
To ascertain that penA is the major player in β-lactam resistance in strain Bp1651 we constructed a ΔpenA mutant (Bp881) where the gene is deleted from its start to stop codon. When compared to Bp1651, Bp881 was significantly less resistant to CAZ (256-fold) and IPM (64-fold), and also exhibited a significantly decreased MEM MIC (4-fold) (Table 1). The results confirm that in Bp1651 PenA alone is a major player in CAZ and IPM resistance, as well as decreased MEM susceptibility.
To assess whether PenA alone or additional determinants were involved in Bp1651 β-lactam resistance, random transposon mutagenesis was performed. Transposon mutants were screened for no growth on LB plates in the presence of 16 μg/ml CAZ or 2 μg/ml MEM. The screening identified two mutants that grew on neither CAZ nor MEM containing plates (Bp878 and Bp879) and four mutants that grew on CAZ, but not MEM containing plates (Bp875, Bp876, Bp877 and Bp880). The six mutants were further characterized by determining CAZ, MEM and IPM MICs (Table 1), and mapping the respective transposon insertion sites in the Bp1651 genome (Fig. 6). MIC testing revealed that all six mutants tested exhibited decreased CAZ and MEM resistance when compared to Bp1651, but to various degrees. Mutants that did not grow on LB plates containing either CAZ or MEM, contained insertions in penA (Bp878) or a gene annotated as TR70_1911 (Bp879). Both of these mutants exhibited similar decreased resistance to CAZ (64 to 128-fold) as the penA deletion or transposon mutants, but reduced resistance to carbapenems was more pronounced in the TR70_1911 mutant. In the majority of sequenced B. pseudomallei genomes, this gene is annotated as a lipoprotein. Mutants that did not grow on LB plates containing MEM, but grew on CAZ-containing plates exhibited various degrees of reduced carbapenem resistance when compared to Bp1651. The most pronounced differences, a 16-fold MIC reduction, with MEM and IPM were observed with mutants Bp875 and Bp876. Bp875 contained an insertion in an intergenic region between a gene encoding a hypothetical protein and putative flgN, encoding a flagellum assembly protein. Bp876 is a mutant with an insertion in dacC (D-ala-D-ala-carboxypetidase or PBP6), indicating that both MEM and IPM interact with this PBP6 in B. pseudomallei. Both mutants also exhibited a significantly (32-fold) reduced CAZr. Insertions in Bp877 and Bp879 exhibited the least pronounced differences in their susceptibilities to CAZ and carbapenems. Bp877 contains an insertion in the gene for squalene hopene synthase, which catalyzes cyclization of squalene to hopene in the bacterial hopanoid biosynthetic pathway21,22. Bp879 contains an insertion in a gene encoding a hypothetical protein.
B. pseudomallei PenA β-lactamase possesses several unique molecular features. Unlike most β-lactamases the enzyme is a membrane-bound lipoprotein23 secreted via the twin arginine transport system24. These unique features extend to the transcriptional regulation of penA expression.
RT and RT-qPCR analyses showed: (1) In wild-type strains penA is co-transcribed with the upstream nlpD1 gene. The nlpD1-penA transcript likely originates from a promoter located upstream of nlpD1. Strains carrying mini-Tn7-penA’-lacZ constructs containing either 336 bp (Bp82.372) or 42 bp (Bp82.364) of the nlpD1 3′ terminus both expressed low levels of β-Gal activity indicating absence of a promoter in this region. NlpD1 is expressed during human infection as the protein is reactive against melioidosis patient sera25; (2) although nlpD1 and penA are co-transcribed from likely the same promoter, nlpD1 transcript levels in LB grown cells are very low when compared to penA. A plausible explanation for this observation is that the penA portion for the nlpD1-penA transcript is more stable than the nlpD1 portion, in part perhaps mediated by the stem-loop structure of TERM 264. A stem-loop structure in the 5′-UTR of Serratia marcescens ampC mRNA was shown to be involved in increased transcript stability26. The resulting PenA levels are sufficient for achieving in wild-type B. pseudomallei strains clinically significant intrinsic resistance to β-lactam antibiotics such as amoxicillin and carbenicillin, but not CAZ and carpapenems.
In this study we confirm for the first time in B. pseudomallei that the conserved G to A transition at position −78 upstream of the penA start codon that has been documented in CAZr clinical isolates10,11,12 indeed generates a constitutive promoter (PA). This leads to transcriptional penA overexpression and thus increased β-lactam resistance, including clinically significant CAZr. Our data are consistent with the previous finding that in a laboratory-selected CAZr resistant B. thailandensis mutant this mutation leads to increased penA transcription by generating a −10 region that is closer to consensus (Fig. S1)27. Clinical B. pseudomallei isolates with PA alone exhibit CAZr, but at lower levels. In contrast, clinical isolates with high-level CAZr frequently contain PA and PenA amino acid substitutions10,12.
We also show that the stem-loop structure in the penA 5′-UTR annotated as Rho-independent TERM 264 terminates transcription from a strong constitutive B. thailandensis promoter and that promoter placement in close proximity to TERM 264 interferes with terminator function. These findings support the notion that the conserved location of the G to A transition, and thus PA, immediately adjacent to TERM 264 in both B. pseudomallei and B. thailandensis (Fig. S1), possibly also interferes with terminator function. Repeated attempts aimed at determining the transcriptional start site of penA in mutants containing PA failed because of the inability to overcome the secondary structure formed by TERM 264 sequences.
Overall, the scenario with nlpD1-penA is reminiscent of E. coli AmpC β-lactamase regulation. Inherited β-lactam resistance can either be due to promoter mutations or mutations affecting the ampC attenuator28,29. The ampC attenuator is the transcriptional terminator of the upstream frd operon and the ampC promoter is located in the last gene of the frd operon30. In this bacterium, AmpC is non-inducible, but is regulated via promoter and attenuator mechanisms, e.g. growth rate dependent anti-termination28. Although not yet tested, a similar mechanism of anti-termination could be at play in regulation of B. pseudomallei penA expression.
We presently do not understand whether penA expression is subject to regulation by factors other than promoter mutation and attenuation. Two mechanisms of induction of β-lactamase activity in Gram-negative bacteria have been described so far, sensing of β-lactams by a histidine kinase and induction of β-lactamase via a response regulator31 and sensing of cell wall damage and induction by cell wall components32. In Gram-negative bacteria, direct sensing of β-lactam antibiotics via sensor kinase is rare and the predominant mechanism of regulation of production of β-lactamase (e.g. AmpC) is induction in response to cell wall damage by β-lactam antibiotics32. Such damage results in release of N-acetylglucosamine-anhydro-N-acetylmuramic acid-oligopeptide. The GlcNAc-anhydro-MurNAc-oligopeptide is transported into the cell by AmpG. After release of GlcNAc, the cytoplasmic anhydro-MurNAc-oligopeptide complexes with the LysR-type transcriptional regulator AmpR to activate transcription of ampC. The inducing activity of anhydro-MurNAc-oligopeptide is modulated by AmpD, a cytosolic N-acetyl-anhydromuramyl-L-alanine amidase that cleaves the oligopeptide stems from the muropeptide. While the AmpR-AmpC system is present in B. cenocepacia (PenR-PenB), it is absent from B. pseudomallei7. Although B. pseudomallei genomes encode potential orthologs of AmpD (e.g. K96243 BPSL2993) and AmpG (e.g. 1710b BURPS1710b_037533, their role in β-lactam resistance, if any, remains unknown; it may be restricted to cell wall recycling32,34. We previously noted that deletion of two LysR-type regulators present upstream of nlpD1 and downstream of penA neither affected penA expression nor susceptibility to β-lactam antibiotics that are substrates of PenA24.
We also do not yet understand whether the transcriptional association of penA with nlpD1 is coincidental or implies a yet to be discovered functional relationship. In E. coli18 and other Gram-negative bacteria, e.g. Vibrio cholerae35, NlpD and EnvC are two non-redundant activators of periplasmic amidases (three in E. coli, AmiA, AmiB and AmiC, and one in V. cholerae, AmiC) that are involved in cleavage of septal peptidoglycan, a step required for daughter cell separation in the final stages of cell division. Whereas no EnvC has been annotated in B. pseudomallei, the bacterium’s genome contains two nlpD genes, nlpD1 located upstream of penA and nlpD2, located in the same genomic context as in E. coli, i.e. upstream of rpoS (Fig. S2). NlpD1 and NlpD2 contains the three motifs/domains also found in the E. coli NlpD homolog: (1) a lipoprotein signal sequence for outer membrane localization; (2) the lysine motif (LysM) that is common in cell envelope-associated proteins and involved in peptidoglycan-binding activity; and (3) the degenerate LytM (dLytM) domain that is required for the protein’s cell wall hydrolytic amidase activating activity18 (Fig. S3). Although related to metalloproteases, the carboxy-terminal dLytM domains of NlpD1 and NlpD2, like E. coli EnvC and NlpD, are missing the residues required for coordination of the catalytic Zn2+ ion (Fig. S4)36. Purified NlpD1 exhibited no protease activity37. Although these observations show that NlpD1 is likely a functional amidase activator with properties found in E. coli NlpD, a role in β-lactamase regulation is not readily evident. Curiously, the amidase it regulates, likely putative AmiC (e.g. BURPS1710b_1064 in 1710b or BPSL0859 in K9624333), is functionally equivalent to AmpD. Although localized in two separate cellular compartments, both AmpD and AmiC release peptide side chains from the glycan strand by hydrolyzing the amide bond between the N-terminal-L-alanine of the oligopeptide chain and MurNAc34.
Although our data clearly show that β-lactam resistance in Bp1651 is largely due to transcriptional overexpression of PenA at the transcriptional and post-transciptional levels, coupled with PenA structural mutations, this resistance is multifactorial and extends beyond PenA. In this strain, a low-molecular weight penicillin binding protein (PBP6) seems to play a significant role in carbapenem resistance, and to a lesser degree CAZr, perhaps by affecting the balance of muropeptides in the cell wall metabolite recycling pool that has previously been shown to affect β-lactamase expression levels in several Gram-negative bacteria34. Inactivation of TR70_1911, a gene encoding a lipoprotein also had a profound effect on carbapenem and CAZr, perhaps because interruption of this gene compromises cell envelope integrity. In Bp1651, the gene is annotated as LptE lipopolysaccharide assembly lipoprotein. However, this annotation is likely incorrect because LptE is an essential protein, but we readily obtained a knockout mutant. Interference with the bacterial hopanoid biosynthetic pathway had a minor yet noticeable effect on CAZ and carbapenem resistance. Hopanoids are bacterial membrane triterpenoid lipids that are involved in modulation of membrane stability and impermeability of the bacterial membrane. In B. cenocepacia38, B. pseudomallei39 and B. thailandensis40 studies with mutants affecting various enzymatic steps in hopanoid biosynthesis and export show that these lipids confer resistance to multiple stresses, including detergent and antibiotic exposure. Intriguingly, an insertion in an intergenic region between a gene encoding a hypothetical protein and flgN, the gene for a flagellum assembly protein, profoundly affected carbapenem resistance and to a lesser degree CAZr. A recent report indicated that decreased MEM susceptibility in clinical isolates is frequently associated with regulatory mutations that lead to increased expression of efflux pumps of the resistance nodulation cell division (RND) family41. Most often affected is amrR, the gene coding for the TetR family repressor of the operon encoding the AmrAB-OprA pump. We did not see such mutations in our transposon mutant screen because this efflux pump is not functional in Bp1651 due to a mutation affecting AmrB12.
Although the overall transcriptional organization of the penA locus and its regulatory elements are conserved among members of the B. pseudomallei group, the divergence observed with the commonly used B. pseudomallei surrogate B. thailandensis E264 is obvious but its significance, if any, remains to be assessed.
Materials and Methods
Bacterial strains and growth
Escherichia coli strains used in this study were DH5α (ThermoFisher Scientific, Waltham, MA) and XL1 Blue (Agilent Technologies, Santa Clara, CA) for routine cloning and RHO3 for conjugation42. B. pseudomallei strains used in this study were Bp8243 and its ∆(amrAB-oprA) derivative Bp82.278, and the virulent strains 1026b11 and Bp165112. Bp82 is an attenuated derivative of 1026b11 and excluded from Select Agent regulations (www.selectagents.gov/SelectAgentsandToxinsExclusions.html). Strains derived from Bp1651 and Bp82.27 are listed in Tables 1 and 2, respectively. All experiments with strain Bp82 and its derivatives were conducted at biosafety level 2 (BSL-2) with Institutional Biosafety Committee approval. Virulent B. pseudomallei strains were manipulated in Select Agent-approved BSL-3 facilities in the Rocky Mountain Regional Biosafety Laboratory at Colorado State University or at the University of Florida, using approved Select Agent-compliant procedures and protocols. Bacteria were routinely cultured in Lennox LB medium (5 g/L NaCl) at 37 °C with aeration. Bp82-derived strains were grown in LB broth or on LB agar plates supplemented with 40 or 80 μg/ml adenine, respectively. Antibiotics were used at the following concentrations: 100 μg/ml ampicillin (Ap) and 35 μg/ml kanamycin (Km) for E. coli; 350 μg/ml Km for B. pseudomallei wild-type strains and 15 μg/ml gentamicin for Δ(amrAB-oprA) strains. YT medium with 15% sucrose and 50 μg/ml 5-bromo-4-chloro-3-indoxyl-β-D-glucuronide (X-Gluc) (Gold Biotechnology, St. Louis, MO) was used for resolution of merodiploids during allelic exchange. For β-galactosidase based blue-white screening agar plates were supplemented with 50 μg/ml 5-bromo-4-chloro-3-indoxyl-β-D-galactopyranoside (X-Gal) (Gold Biotechnology).
B. pseudomallei strains were grown in cation-adjusted Mueller-Hinton II broth (MHB) (Becton Dickinson and Company, Sparks, MD) supplemented with 40 μg/ml adenine for Bp82 derivatives. The MICs of antibiotics were determined by the standard microdilution method, following CLSI guidelines44.
Construction of strains with chromosomally integrated penA’-lacZ transcriptional fusions
A 262 bp fragment encompassing 42 bp of the 3′ terminus of nlpD1, the 174 bp nlpD1-penA intergenic region (IR), and 22 bp of the 5′ terminus of penA was PCR amplified from genomic DNA of 1026b and Bp1651 using P2978 & P2994 (primers were purchased from Integrated DNA Technologies, Coralville, IA and are listed in Table S1), and Q5 High-Fidelity DNA Polymerase according to the manufacturer’s recommendations (New England Biolabs, Ipswich, MA.). Similarly, a 556 bp PCR fragment containing an extended 336 bp nlpD1 3′ terminus, the 174 bp nlpD1-penA IR, and 22 bp of the 5′ terminus of penA was PCR amplified from genomic DNA of 1026b using P3005 & P3004 and Q5 High-Fidelity DNA Polymerase.
For construction of delivery vectors containing transcriptional lacZ fusions on mini-Tn7 elements, the PCR fragments were digested with SpeI + HindIII and 250 bp fragments ligated between the same sites of pUC18-mini-Tn7T-Gm-lacZ45 to yield pPS3299 (1026b insert) and pPS3300 (Bp1651 insert). Similarly, a 544 bp SpeI-HindIII fragment containing an extended nlpD1 3′ terminus was cloned into pUC18-mini-Tn7T-Gm-lacZ to form pPS3319. The 262 bp PCR fragment derived from the 1026b template was also cloned into pGEM-T Easy (Promega, Madison, WI) that was A tailed with Taq polymerase (New England Biolabs) to obtain pPS3295. This plasmid was used as the template for introduction of single nucleotide changes present in the IR of Bp1651 but not 1026b (i.e. −41T, −78A and −171C; coordinates are relative to the first penA nucleotide) using mutagenic primers and the QuikChange II kit (Agilent Technologies). Using this strategy, pPS3296 containing −78A in the IR was created with mutagenic primer pair P2990 & P2991, pPS3297 containing −41T with P2992 & P2993, and pPS3313 containing −171C with P3006 & P3007. To derive delivery vectors containing transcriptional lacZ fusions on mini-Tn7 elements, 250 bp SpeI-HindIII fragments were isolated from these plasmids and ligated between the same sites of pUC18-mini-Tn7T-Gm-lacZ to yield pPS3301 (1026b IR with −41T mutation), pPS3302 (1026b IR with −78G mutation), and pPS3318 (1026b IR with −171C mutation).
Mini-Tn7-lacZ constructs were transferred into the Bp82.27 genome by co-electroporation of the delivery vectors with the helper plasmid pTNS3, and insertions were verified by PCR and sequencing using previously described methods46. The strains thus obtained were: Bp82.363 with empty mini-Tn7T-Gm-lacZ; Bp82.364 with mini-Tn7T-Gm-1026b-42 bp nlpD1-IR-penA’-lacZ; Bp82.365 with mini-Tn7T-Gm-Bp1651-42 bp nlpD1-IR-penA’-lacZ; Bp82.366 with mini-Tn7T-Gm-1026b-42 bp nlpD1-41T-IR-penA’-lacZ; Bp82.367 with mini-Tn7T-Gm-1026b-42 bp nlpD1-78A-IR-penA’-lacZ; Bp82.371 with mini-Tn7T-Gm-1026b-42 bp nlpD1-171C-IR-penA’-lacZ; and Bp82.372 with mini-Tn7T-Gm-1026b-336 bp nlpD1-IR-penA’-lacZ. The strains contained the mini-Tn7-penA’-lacZ fusion construct at attTn7 glmS3, except Bp82.371 and Bp82.372, which had the fusion constructs inserted at attTn7 glmS2.
For terminator activity assays, gBlocks® Gene Fragments were designed and purchased from Integrated DNA Technologies. These fragments contained the constitutive B. thailandensis S12 promoter (PS12)46, 93 bp (with terminator TERM264) or 44 bp (without TERM 264; ΔTERM 264) penA upstream region, the first 22 bp of penA, and 92 bp of inert “spacer DNA”. The first set of gBlocks contained PS12 centered 134 bp upstream of TERM 264 or its deletion junction. The respective 274 bp and 225 bp double-stranded gBlocks were cloned into A tailed pGEM-T Easy to obtain pPS3322 and pPS3323, respectively. The second set of gBlocks were of the same size and sequence as the first set but contained PS12 centered 24 bp upstream of TERM264 or its deletion junction. The respective gBlocks were cloned into A tailed pGEM-T Easy to obtain pPS3344 and pPS3349, respectively. To derive delivery vectors containing transcriptional lacZ fusions on mini-Tn7 elements, 262 bp (with TERM 264) or 213 bp (ΔTERM 264) SpeI-HindIII fragments were isolated from these plasmids and ligated between the same sites of pUC18-mini-Tn7T-Gm-lacZ to yield pPS3331 (PS12 134 bp upstream of TERM 264), pPS3332 (PS12 134 bp upstream of ΔTERM 264), pPS3345 (PS12 24 bp upstream of TERM 264), and pPS3350 (PS12 24 bp upstream of ΔTERM 264). Mini-Tn7-lacZ constructs were transferred into the Bp82.27 genome, and insertions were verified by PCR and sequencing. The strains thus obtained were: Bp82.363 with empty mini-Tn7T-Gm-lacZ; Bp82.374 with mini-Tn7T-Gm-134 bp-TERM 264-penA’-lacZ; Bp82.375 with mini-Tn7T-Gm-134 bp-ΔTERM 264-penA’-lacZ; Bp82.380 with mini-Tn7T-Gm-24 bp-TERM 264-penA’-lacZ; and Bp82.381 with mini-Tn7T-Gm-24 bp-ΔTERM264-penA’-‘lacZ. All strains contained the mini-Tn7-penA’-lacZ fusion construct at attTn7 glmS3.
Construction of a translational penA’-‘lacZ fusion
To assess functionality of penA transcription and translation initiation signals in E. coli, a penA’-‘lacZ translational fusion was constructed. This was achieved by cloning a 292 bp HindIII-SalI fragment from pPS3296 containing the 174 bp 1026b IR with the −78A mutation between the same sites of the translational fusion vector pPZ1047. This step fused the first 7 amino acids of PenA in-frame to LacZ and placed the expression of the fusion protein under the control of the PenA transcription and translation initiation signals.
β-Galactosidase (β-Gal) activity was measured, and activity units were determined by the Miller method using LB-grown mid-log phase bacterial cultures in which cells were made permeable by SDS and chloroform treatment48.
Bacteria were grown at 37 °C in LB medium to the mid-log phase (OD600nm = 0.6 to 0.8). Total RNA was isolated using the RNeasy Protect Bacteria mini kit (Qiagen, Valencia, CA) and cDNA synthesis was performed as previously described49,50. The transcript containing the 3′ end of nlpD and the 5′ end of penA was amplified using Q5 High-Fidelity Polymerase according to the manufacturer’s recommendations using primers P2999 & P3003. Copy DNAs derived from transcripts internal to nlpD1 and penA were amplified with P2997 & P2998 and P3001 & P2293), respectively. Genomic DNA isolated from 1026b cells using the PureGene Core kit A (QIAGEN, Valencia, CA) was used as a positive control reaction, while DNAse treated cDNA generated from 1026b and Bp1651 RNA and sterile water were used as negative control templates.
Cells were grown to mid-log phase and total RNA was extracted as described above. cDNA synthesis and reverse transcription-quantitative PCR (RT-qPCR) was performed as previously described49,50 to assess expression levels of penA and nlpD1 mRNAs. 23S rRNA was used as the housekeeping control. The primer sets used were P2988 & P2989 for 23S rRNA49, P2853 & P2854 for penA, and P3008 & P3009 for nlpD1.
Construction of a markerless chromosomal penA deletion
A construct for isolation of a markerless chromosomal penA deletion was assembled in several steps. Using genomic DNA of Bp1651 as a template and Q5 High-Fidelity DNA polymerase two partially overlapping PCR fragments were obtained: a 610 bp fragment using the upstream primer P2917 & SOEing primer P2918 and a 747 bp fragment using SOEing primer P2919 & the downstream primer P2920. The two partially overlapping fragments were then used as templates in a second PCR with Platinum Taq DNA polymerase and P2917 & P2920 to generate a 1,297 bp fragment containing a complete penA deletion including its start and stop codon, flanked by 585 bp of upstream and 712 bp of downstream sequences. This fragment was ligated into pGEM-T Easy to form pPS3401. Next, a 1,325 bp EcoRI fragment was isolated from this plasmid in ligated into the EcoRI site of pEXKm5 to yield pPS3402. This plasmid was then used to delete the 888 bp penA gene in Bp1651 by allelic exchange and sucrose counter-selection as described previously42.
Using a high-efficiency mating protocol originally developed for P. aeruginosa, we performed mutagenesis of strain Bp1651 using Tn5-based transposon T2451. Bp1651 was grown overnight at 37 °C in LB medium. The next day, an equal volume of 20 mM NaNO3 was added and the culture incubated at 42 °C for at least 3 hours. A concentrated aliquot of this culture was then mixed with a concentrated aliquot of log phase donor cells (in this case E. coli RHO3 with T24 delivery plasmid pLG107), followed by overnight incubation at 37 °C. Dilutions were then plated on LB + 300 μg/ml Km to select for transformants containing chromosomally integrated T24 transposons. This method yielded >10,000 colonies per 0.5 ml mating mixture. Kanamycin resistant colonies were picked and arrayed into 96-well plates containing LB + 35 μg/ml Km + 10% glycerol using a QPix2 colony-picking robot (Genetix). Plates were incubated for 36 h at 37 °C, followed by 1–2 h at room temperature, and then stored at −80 °C. For determination of ceftazidime and meropenem susceptibilities bacteria were replicated onto freshly prepared LB plates with 16 μg/ml ceftazidime or 2 μg/ml meropenem.
Mapping of chromosomal T24 insertion sites
T24 insertion sites in ceftazidime and meropenem susceptible mutants were identified by semi-degenerate PCR and sequencing of the transposon-genome junctions as previously described for B. thailandensis with minor modifications52. KAPA polymerase was used for all steps and so pre-PCR was omitted. Template for PCR round 1 was instead obtained by mixing 5 μl of overnight culture with 5 μl of 1xKAPA GC buffer containing 2 mM MgCl2, followed by a 20 min incubation at 98 °C in a thermocycler. One μl of the cell lysate was then used as template in PCR round 1. After PCR round 2 and clean up, 2–4 μl of the clean-up product were mixed with 2.5 μl of 5 μM lacZ124L2 sequencing primer (5′-CAGTCACGACGTTGTAAAACGACG), and the volume was brought up to 20 μl with sterile water. Transposon-chromosomal junction DNA sequences were determined by Sanger sequencing. Transposon insertion sites were identified by BLAST searches against the Bp1651 genome sequence (Genbank accession numbers CP012041.1 and CP012042.1).
CLUSTAL multiple sequence alignment was performed by MUSCLE (3.8) on the European Bioinformatics Institute webserver at https://www.ebi.ac.uk/Tools/msa/muscle/. The RNA secondary structure plots were generated and free energy values calculated using the RNAfold WebServer (http://rna.tbi.univie.ac.at/cgi-bin/RNAWebSuite/RNAfold.cgi). BPROM available at www.softberry.com was used for promoter prediction. Terminator prediction was according to Kingsford et al.53.
The datasets generated and/or analyzed during the current study are available from the corresponding author upon reasonable request.
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Work in the laboratories of H.P.S. and A.T. was supported by University of Florida start-up funds and by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under Award Number R21 AI128518. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Support for aspects of this project was also provided via contract HHSO100201400002C from the United States Biomedical Advanced Research and Development Authority (BARDA) to The Medicines Company.