Algal-bacterial interactions include mutualism, commensalism, and predation. However, how multiple environmental conditions that regulate the strength and prevalence of a given interaction remains unclear. Here, we test the hypothesis that the prevailing algal-bacterial interaction shifted in two years (2005 versus 2015), due to increased temperature (T) and Saharan dust depositions in high-mountain lakes of Sierra Nevada (S Spain). Our results support the starting hypothesis that the nature of the prevailing algal-bacterial interaction shifted from a bacterivory control exerted by algae to commensalism, coinciding with a higher air and water T as well as the lower ratio sestonic nitrogen (N): phosphorous (P), related to greater aerosol inputs. Projected global change conditions in Mediterranean region could decline the functional diversity and alter the role of mixotrophy as a carbon (C) by-pass in the microbial food web, reducing the biomass-transfer efficiency up the web by increasing the number of trophic links.
Algae and bacteria numerically dominate the ocean and freshwater communities1, comprising the majority fraction of particulate organic carbon2. Hence, the interactions between algae and bacteria are crucial in aquatic environments as they control nutrient cycles and biomass production in the trophic web3. These relationships encompass commensalism4, predation5 and mutualism6, forming a continuum of the interaction modes7. In fact, several studies have reported the commensalistic interaction between the two metabolic groups8,9, where bacteria derive C for growth from the excreted organic carbon (EOC) provided by algae1,10. Thus, bacteria can consume up to 50% of the C fixed by phytoplankton11. In predation (i.e. bacterivory), bacteria supply C and nutrients (N, P or Fe) to mixotrophic algae12,13. Mutualism is established when bacteria, by decomposing organic matter, supply mineral nutrients14 or vitamins15 for algal growth3 while the algae supply C for bacterial growth. Furthermore, a complex mutualistic interaction develops when a dual control acts simultaneously through the bacterial dependence on EOC (i.e. a resource-based control), together with a predatory control by mixotrophic algae16,17.
The nature and strength of these interspecific interactions can change depending on environmental conditions7. Thus, nutrient input can alter the interaction in several ways. For example, moderated nutrient amendments can shift an algal–bacterial interaction from commensalism to competition depending on bacterial N:P ratio18,19, whereas high P enrichment (>30 µg P L−1) favours the development of strict autotrophic against mixotrophic algae, thereby reinforcing commensalistic interaction17. In addition, the strength of algal-bacterial interaction is regulated by T, due to a stimulatory effect on phytoplankton20, which increase EOC, supporting bacterial carbon demand and reinforcing the commensalistic relationship21. These findings contrast with the stronger effect of T on heterotrophic than autotrophic processes predicted by metabolic theory22 (although a recent study23 has questioned this theory), implying that, at the organism level, there is a shift toward heterotrophic metabolism of mixotrophic algae through increased bacterivory24. Therefore, the predominance of strict autotrophs or mixotrophs might encourage commensalistic or bacterivory interaction under warming, with the consequent effect on the regulation of the microbial food web.
Furthermore, warming can strength stratification in lakes, and therefore the exposure of microorganisms to ultraviolet radiation (UVR) in the upper layers of the water column25. It has widely been reported that UVR has a negative impact on several targets and processes (e.g. photosynthesis, and nutrient uptake, primary production (PP) and heterotrophic bacterial production (HBP))26. Nevertheless, it has also been demonstrated that planktonic organisms have a great capacity to acclimatize to high UVR in oligotrophic ecosystems by increasing the percentage EOC27, stimulating the growth of UVR-resistant bacteria28 or increasing the consumption of bacteria by mixotrophic algae16,29.
The joint effect of nutrient enrichment, T, and UVR on algal-bacterial interaction and microbial food web has been reported from experiments in single lakes30,31,32. However, little attention has been placed on the way in which these environmental factors alter the algal–bacterial relationship across systems over the long term. In this context, our study was conducted in high-mountain lakes distributed in an area of 86,200 ha in Sierra Nevada National Park, within the Mediterranean region. The effects of global change are accentuated in this region33, and even more so in high-elevation areas compared to the global average34, due to their greater ecological sensitivity to such change35. Particularly, the Mediterranean region is exposed to the increasingly nutrient-rich aerosol inputs from the Saharan Desert36, and Sierra Nevada is specifically receptive to Saharan dust due to its location and altitude37. In addition, the high elevation implies that the lakes are exposed to high-intensity UVR during the ice-free period, which may prolong over time because of rising T. These characteristics, together with their oligotrophic state and simple trophic web structure, make these lakes particularly sensitive indicators of the past and current global conditions and also serve as models to predict future changes because they register environmental change more directly38, being considered sentinels of global change39.
For these reasons, our objective was to assess whether the regulation of the algal-bacterial interaction has changed in 2015 respect to 2005, after a period in which Saharan dust transport to the Mediterranean basin and air T have increased, while chronic UVR levels remain high. Our hypothesis is that greater dust deposition to Sierra Nevada lakes and higher T have shifted the algal metabolism towards stricter autotrophy and higher PP, and therefore, we expect a reinforcement of the commensalistic interaction between algae and bacteria to the detriment of the predatory interaction.
Remote sensing data
Environmental factors associated with global change have steadily varied over the last 35 years from the south-eastern Iberian Peninsula. While UVR striking the Sierra Nevada Mountains remained high during the summer period (300.25–306.47 W m−2; Supplementary Fig. S1), Saharan dust input and air T followed a positive trend during the 10 years covered by our metabolic measurements (Fig. 1). The UV aerosol index has increased progressively from the 1980s on, both in intensity and frequency (Fig. 1), accompanied by dust inputs during the winter of recent years, which is highly unusual in this region (Fig. 2). Specifically, during the last decade the aerosol index intensity has increased from 0.39 in 2005 to 0.44 in 2015, and the frequency of high-intensity events (aerosol index >1) has increased from 22 in 2005 to 49 in 2015. Also, the mean air T in summer has risen from 15.42 °C in 2005 to 16.45 °C in 2015.
Abiotic and biotic changes in the lakes in 2005 and 2015
Diffuse attenuation coefficient for downward radiation (kd) showed low values of UVR (305 nm) and photosynthetically active radiation (PAR) throughout the water column, with the lakes Gabata, Larga, Caldera, and Yeguas exhibiting greater transparency to UVR (kd UVR305 = 0.31–0.92 m−1), while the lakes Chico de la Virgen, Grande de Rio Seco, and Alta de Río Seco reached the highest values (kd UVR305 = 4.04–7.5 m−1). The mean water-column T for the 10 lakes sampled in both years ranged between 15.5 °C in 2005 and 16.1 °C in 2015, and 60% of the lakes analysed had higher T in 2015, although without significant differences between the two years (t-test p > 0.05).
The trophic status of the lakes sampled in 200521 and 2015 spanned oligo- to mesotrophy, as indicated by the total dissolved P, with values ranging from 0.049 to 0.483 μM of P in the lakes Larga and Aguas Verdes, respectively (Table 1). No significant differences appeared in total dissolved P concentration between the two years (t-test p > 0.05). However, the mean value of the seston N:P ratio for the 10 lakes sampled both years was 48.9, with 50.0% of the lakes showing values higher than 30 in 2005, whereas mean N:P ratio decreased to 18.75 with only 11.1% of the lakes showing higher values than 30 in 2015 (t-test p > 0.05). The mean concentrations of chlorophyll a (Chl a) and dissolved organic carbon did not significantly differ (t-test p > 0.05) between years (3.84 µg Chl a L−1 and 95.0 μM in 2005; 5.41 µg Chl a L−1 and 53.25 μM in 2015; Table 1).
Regarding the functional variables, particulate primary production (PPP) registered a mean value of 1.71 µg C L−1 in 2005, significantly increasing in 2015 (10.85 µg C L−1; t-test, p = 0.049; Table 1 and Fig. 3). However, HBP mean values slightly decreased from 0.13 µg C L−1 h−1 in 2005 to 0.11 µg C L−1 h−1 in 2015, without significant differences between the two years (t-test p > 0.05; Table 1 and Fig. 3). The mean bacterivory rate (BV) was 18.48 ng C L−1 h−1 in 2005 and 9.71 ng C L−1 h−1 in 2015 (Table 1). The BV rates normalized by HBP (%BV) significantly decreased from 31.45% (2005) to 9.08% (2015 (t-test p = 0.032, Fig. 3).
Factors controlling the algal-bacterial interaction in 2005
The observed variability of PPP of lakes during 2005 was explained by sestonic P (61.3% of variance; Table 2), according to the characteristic P limitation of the lakes (mean seston N:P ratio = 44.13). By contrast, water T, UV mean irradiance at 305 nm wavelength received throughout the water column (Im305), and the ratios between Im305 and T (Im305:T) or Im305 and total P (Im305:total P), failed to explain the PPP during 2005 (Fig. 4a,b).
HBP was explained by Chl a (44% of variance), Im305 (32.9%) and total dissolved P (19.2%; Table 2). The single regression assessing the C control on HBP showed a significant positive relation between EOC vs. HBP (Fig. 5c; r2 = 0.44; β-slope = 0.662; p < 0.05), and dissolved organic carbon vs. HPB (r2 = 0.76; β-slope = 0.873; p < 0.05); this appears to indicate that the C-control of HBP is exerted mainly by allochthonous C.
The %BV was explained by seston N:P (60.8%, p < 0.05, Table 2), suggesting that bacterivory was predominant in lakes with P impoverished seston. The single regression assessing the abiotic control (UVR, total dissolved P, and water T) on the %BV showed a positive relation between Im305: total dissolved P vs. %BV (r2 = 0.39; p < 0.05) but negative between ImPAR:T vs. %BV (r2 = 0.38; p < 0.05; Fig. 4c,d).
The high BV rates found for this year were reflected in a negative exponential relation (r2 = 0.34; p < 0.05) between HBP and %BV, suggesting a bacterivory control of algae on bacteria (Fig. 5a). Moreover, the lack of positive relationship between bacterial abundance and HBP (p > 0.05; Fig. 5b) is consistent with a top-down control.
Factors controlling the algal-bacterial interaction in 2015
In 2015, water T explained the PPP variance (75.5%, Table 2) and their relation was direct. Also, in contrast to 2005, the single regression assessing the abiotic control showed an exponential negative relation between Im305:T or Im305: total P and PPP (Fig. 4e,f), according to a negative relation of Im305, but positive of P (Supplementary Fig. S2) or T on PPP (Table 2).
Total primary production (TPP) and total P explained 70.6% and 19.6% of HBP variance, respectively (Table 2). The high relation between TPP and HBP is consistent with a significant direct C control between EOC and HBP, but not dissolved organic carbon (Fig. 5c,d). These findings support the contention that EOC rather than dissolved organic carbon controlled bacterial growth (bottom-up control; Fig. 5c,d), suggesting a reinforcement of the algal-bacterial commensalism in 2015.
Sestonic N:P ratio and Im320: total P were positively related to %BV, explaining 40.5% and 11.2% of %BV variance, respectively (Table 2, see also Fig. 4h), and Chl a was negatively related to %BV, explaining 38.9% of %BV variance (Table 2). These findings indicate that greater P availability among lakes simultaneously stimulated the Chl a concentration and weakened bacterivory.
The low %BV values found in 2015 (Fig. 3) together to the absence of bacterivory control between HBP and %BV (Fig. 5a) and a significant positive relationship (r2 = 0.63; p < 0.05) between bacterial abundance and HBP (Fig. 5b), consistently suggest a weak consumption of bacteria by the algae.
The main finding of our study was a shift in the nature of the interaction between algae and bacteria in high-mountain lakes of Sierra Nevada in 2005 and 2015, with a consistent and greater predominance of autotrophic metabolism in 2015 supported by an increase in the PPP, and with the reinforcement of commensalistic algal-bacterial interaction against the weakening of bacterivory control (Fig. 6). This supports our hypothesis and highlights that temperature increase and a dustier world40 like observed in 2015, can alter the functioning of high mountain lakes where BV rates strongly regulate the dynamics and structure of the microbial community.
The BV rate was altered in the two years of our analysis. In 2005, the predatory control by bacterivores rose to some 30%, this becoming a characteristic feature of the high-mountain lakes of Sierra Nevada. This feature is supported by predominance of mixotrophic organisms in Sierra Nevada lakes4,16. Field studies have revealed that mixotrophic algae are often numerically dominant in freshwater systems and can exert greater grazing impact on the bacterial community than can heterotrophic phagotrophs29,41. Concretely, in 2005 the bacterivory of mixotrophs could constitute an advantageous nutritional mode against strict metabolisms under the typically low P concentration of Sierra Nevada Lakes (see Table 2)17,30 as has also been observed in other lakes with low P values42,43. In addition, the fact that %BV was positively correlated to Im305:Total dissolved P ratio supports the idea that mixotrophy is an adaptive strategy to low P levels and also high UVR levels29,44. The great adaptation of mixotrophs to high light intensity could be related with its capacity to grow as photoheterotrophs45, reducing the costs of investment in photoprotective mechanisms for photosystem II. Several studies have demonstrated increased grazing rates by mixotrophs in the epilimnion under high irradiance levels16,46. The reported adaptation of mixotrophs to low nutrient conditions5,44 is supported by loss of mixotrophs after a strong P-pulse as has been demonstrated experimentally in Sierra Nevada lakes17,30, in Andean lakes29, in tropical and temperate lakes47, and in the laboratory48 as well as through studies of gradients of increasing trophic state49.
In fact, in 2015, the %BV fell as much as 12.85% (Fig. 3) as the dust input intensified (Fig. 1), the sestonic N:P ratio diminished (Table 1), and concomitantly mixotrophs represented only 19.3% of the algae community as opposed to 61% of strict autotrophs in the ensemble of lakes (Supplementary Fig. S3). The %BV reduction implied a weakening in bacterivory control with respect to 2005 (Fig. 5a), which was corroborated by a bacterial abundance:HBP coupling in 2015 (Fig. 5b). In this sense, in a coastal upwelling, it has been reported that when bacterial mortality by predation is low, all HBP can be converted into BA, and conversely, the absence of a bacterial abundance:HBP coupling is indicative of a strong bacterivory control50.
Contrary to 2005, in 2015 the predominant algal-bacterial interaction was commensalism, led by higher PPP (and Chl a) (Fig. 3). The PPP was triggered mainly by water T (Table 2) concomitantly with an increasing trend in aerosol dust intensity and air T on Sierra Nevada from 1980 to 2015 (Fig. 1). It is known that P, Fe, and other limiting nutrients contained in aerosol dust affect the phytoplankton physiology51 and enhance PPP of lakes17 and coast52. The greater aerosol dust inputs to Sierra Nevada during the recent years of our study translated into a lower mean N:P ratio in 2015 and a lower percentage of lakes with a N:P ratio higher than 30 (see Results and Table 1), indicating P incorporation into seston, as also observed in previous studies19. The positive effect of nutrient-rich dust input in autotrophy could be enhanced by a stimulatory effect of T on phytoplankton photosynthetic yield53 and an attenuation of UVR inhibition on autotrophs54. Furthermore, rising T favoured the dominance of strictly autotrophic phytoplankton taxa, such as Chlorophyta55.
Supporting the advantage of strict autotrophy after greater aerosol inputs and higher water T, we observed an EOC increase proposed as a protective mechanism to prevent the photosystem damage under high light irradiance56,57. Furthermore, the EOC increase determined a reinforcement of commensalistic algal-bacterial interaction, since EOC was the main C source regulating HBP in 2015 whereas dissolved organic carbon was the source in 2005 (Fig. 5c,d and Fig. 6). In agreement with our results, an experiment in marine waters58 showed that the direct C coupling between bacteria and phytoplankton exudates was greatest when the nutrient concentration was higher and grazing was lower, coinciding with a predominance of small autotrophic cells as the main components of the phytoplankton community.
The high UVR-adaptation of the algae inhabiting high-mountain lakes together with the rising water T and greater nutrient inputs linked to dust deposition that occurred in 2015 shifted the predominant algal-bacterial interaction from bacterivory to comensalism59. While we cannot establish a consolidated trend from our study, it is true that the projections of the climatic change for the present century could be more similar to those conditions that occurred in 2015. Thus, the loss of a functional trait such as mixotrophy in high-mountain lakes could deteriorate the functional biodiversity29,60 and biogeochemistry of these remote and fragile ecosystems, by altering processes such as C and nutrient cycles due to the disconnection between the microbial loop and higher trophic levels. Thus, the decrease in mixotrophs is expected to weaken the C bypass towards the grazing chain of the lakes29,46, promoting the development of heterotrophic microbial food web and diminishing the energy-transfer efficiency due to a greater number of links (Fig. 6)16,61.
Material and Methods
The remote-sensing data for the Sierra Nevada area were gathered from 1980 to 2015. Daily data of the area-average aerosol index, air T, and surface UVR fluxes in this region were downloaded from Giovanni v. 4.18.362. Aerosol index data were taken from the Total Ozone Mapping Spectrometer (TOMS) Nimbus-7 (March 21, 1979 – May 5, 1993), TOMS Earth Probe (July 22, 1996 – June 28, 2005) and the Ozone Monitoring Instrument (OMI) (June 29, 2005 – December 31, 2015) satellites (data from 1993 to 1996 are not available), while T and surface UVR-flux data came from the MERRA and MERRA-2 model, respectively. Aerosol index intensity was calculated as the mean value of aerosol index for each entire year, whereas the frequency of high load aerosol index events was calculated as the number of days per year with aerosol index >1.
Our study was conducted in 14 lakes in 2005 and 13 in 2015, which 10 lakes were common between the two years. Some of the lakes could not be sampled in 2015 due to the severe hydric stress to which are subjected, affected by the Mediterranean climate. All lakes are located above the tree line in the Sierra Nevada Mountains (36.8559–37.8159 N, 2.8319–3.8409 W), in the southern Iberian Peninsula (Supplementary Fig. S4). Sampling was conducted during the ice-free period (mid-July to mid-August) of each year.
These lakes are oligo- and mesotrophic (Chl a range from 0.25 to 12.66 µg L−1). The external inputs of mineral nutrient occur mainly during thaws, and are associated with sporadic events of Saharan dust deposition63,64 containing high P levels, with a mean molar total N:total P ratio in total dust deposition ranging from 10 to 5037. The lakes are characterized by their small size (<3 ha), high transparency, shallow water column (<10 m maximum depth) and by the simplicity of the pelagic fishless community, with a low abundance of heterotrophic nanoflagellates and ciliates, and the presence or dominance of mixotrophic protists in the trophic web4.
From each lake, an integrated sample representative of the mixing layer of the water column was collected using 10 L-Niskin Bottle and used for biological and chemical analyses. The samples were then filtered through a 45-µm pore-size mesh to remove zooplankton, and subsamples for each variable analysed were taken in triplicate (see below).
Morphometric and physical measurements were also performed for characterizing each lake (see below).
Temperature and light measurement
Vertical profiles of radiation and T of water column were determined at noon using a submersible radiometer BIC (Biospherical Instruments Inc., CA, USA) that registered measurements of downwelling irradiance at wavelengths representative of the different regions of the solar spectrum (305, 320, and 380 nm and full PAR [400–700 nm]). The kd were determined from the slope of the linear regression of the natural logarithm of downwelling irradiance vs. depth for each wavelength.
Im for PAR and UVR was calculated as in equation (1):
where I0 is the mean incident surface irradiance; kd is the mean attenuation coefficient for UVR at 305, 320, 380 nm and PAR; and z is the maximum depth for each lake.
The concentrations of total P and total dissolved P were determined in 25-ml aliquots after digestion with a mixture of potassium persulphate and boric acid at 120 °C for 30 min65. Total N and total dissolved N samples were also digested with persulfate and measured as nitrate following the ultraviolet spectrophotometric screening method65. For determination of total dissolved N and total dissolved P, the water samples were previously filtered at low pressure (<100 mmHg) using glass-fibre filters (0.7 µm pore-size, Whatman GF/F). To determine sestonic C, N, and P, water samples (0.5 L for C/N and 0.5 L for P) were filtered through precombusted (1 h at 550 °C) glass-fibre filters of 1-µm pore size (Whatman GF/B) at low pressure (<100 mm Hg). The filters were immediately frozen at −20 °C, and the C and N analyses were performed using a Perkin-Elmer 2400 elemental analyser. The sestonic P was determined with the same method as that described for total P.
For dissolved organic carbon determination, samples were filtered through pre-combusted (2 h at500 °C) glass fibre filters (Whatman GF/F) and acidified with HCl 1 N. The analyses were carried out in a total organic carbon analyzer (TOC-VCSH/CSN Shimadzu).
Chl-a concentrations were determined by fluorometric techniques66. The samples were filtered onto a Whatman GF/F filter (0.7 µm pore size) and the photosynthetic pigments extracted in 5 ml of absolute methanol at 4 °C in darkness.
Samples were measured at an excitation wavelength of 460 nm and emission at 670 nm, with a fluorometer LS55 Luminescence Spectrometer (Perkin-Elmer, Boston, MA, USA). Previously, a calibration curve was made with pure chlorophyll spinach extract to transform fluorescence values in chlorophyll concentration.
Following the 14C method proposed by Steeman-Nielsen67, 140-ml samples from each lake were collected for PP measurements. Four 35-mL Teflon flasks for each treatment (three clear and one dark as control) were added with 9.25 MBq of NaH14CO3 (specific activity: 310.8 MBq mmol-1, DHI Water and Environment, Germany). The flasks were incubated for 4 h around noon, and the laboratory procedure was based on Carrillo et al.56. Thus, 4-ml aliquots were taken before filtration to measure the TPP produced, and 35-ml aliquots were filtered, retaining the organic carbon particles in Nuclepore filters of 1-µm pore size to determine the PPP. From the filtrates (<1 μm), 4 ml were used for measuring the EOC.
Filters and filtrates were placed in 5- and 20-mL scintillation vials, respectively, and acidified with 100 µL of 1 N HCl in order to remove DI14C. Vials were then kept open during 24 h in an aeration hood following the recommendations of Lignell68. Then, the vials were filled with scintillation cocktail (Ecoscint A) and counted using a scintillation counter (Beckman LS 6000TA) equipped with autocalibration.
The sum of EOC and PPP corresponds with the TPP. The % EOC was calculated as in equation (2):
Abundance/biomass of planktonic organisms
Abundance and biomass of algae, heterotrophic nanoflagellates, ciliates, and nanoplankton were counted at 400x and 1000x magnification under an inverted microscope (Carl Zeiss AX10, LCC, USA).
For the quantification of autotrophic picoplankton and bacteria69, several 1.5 mL aliquots from each sample were fixed with paraformaldehyde (1% final concentration) and immediately stored at −180 °C until further processing. Autotrophic picoplankton abundance was quantified based on Chl a auto-fluorescence, whereas total picoplankton abundance was quantified after other 1.5-mL fixed aliquots were stained with SYBR Green I DNA (Sigma-Aldrich) 1:5000 final dilution of initial stock. Absolute cell-abundance values were determined using a Becton Dickinson FACScan flow cytometer (Oxford, UK) and Yellow-green-l µm beads (Fluoresbrite Microparticles, Polysciences, Warrington, PA, USA).
Heterotrophic bacterial production
HBP was determined as incorporation of 3H-thymidine (specific activity = 52 Ci mmol−1, Amersham Pharmacia) into the bacterial DNA. Briefly, 3H-thymidine was added to sets of six (four replicates plus two blanks per lake) sterile microcentrifuge tubes filled with 1.5 mL of sample to a final (saturating) concentration of 25 nM. The vials were then incubated at in situ T in darkness for 1 h. After incubation, the incorporation of 3H-thymidine was stopped by adding trichloroacetic acid (TCA, 5% final concentration). Likewise, blanks were TCA-killed before the radiotracer was added. After the cold 5% TCA extraction, the precipitate was collected by centrifugation at 16,000 g for 10 min, rinsed (and centrifuged) twice with 5% TCA, and measured in a scintillation counter equipped with autocalibration (Beckman LS 6000 TA). The conversion factor 1.5 × 1018 cell mol−1 was used to estimate the number of bacteria produced per mole of incorporated 3H-thymidine70. The factor 20 fg C cell−1 was applied to convert bacterial production into C71.
BV was determined from the amount of bacteria traced with 3H-thymidine that were captured by algae following the method proposed by Medina-Sánchez16. For determining BV, sets of four Teflon bottles (three replicates and one blank per lake) were filled with 25 ml of water samples each, radiotraced with 3H-thymidine to a final concentration of 25 nM, and incubated for 1 h in full sunlight. After incubation, the bacterivory was stopped by adding neutralized formaldehyde (0.75% w/v final concentration). Likewise, blanks were formaldehyde-killed before the incubation with the radiotracer. Aliquots of 1.5 ml were obtained from each replicate for determining total activity in the sample. The remaining sample volume (23.5 ml) was filtered through 1 µm pore-size cellulose nitrate filter (Sartorius). Aliquots of 1.5 ml were taken from the filtrate to determine the residual activity, while the activity registered on the filter served to determine BV. The filters were dissolved using acetone 100% and then subjected to centrifugation at 16,000 g for 10 min at 4 °C. Supernatant acetone was removed, and the pellet was re-suspended with 5% TCA (1.5 ml). The subsequent procedure was similar to HBP. %BV was calculated as in equation (3):
where bacterivory was determined from the bacterial incorporation rate by fraction >1 µm retained on the filter. Bacterivory values were converted to cell number and bacterial C captured using the same conversion factors as for HBP determination.
Paired t-test for dependent samples analysis was carried out with the 10 lakes that were sampled both years (2005 and 2015) to determine significant differences in Chl-a, PPP, HBP, %BV, sestonic N:P ratio, dissolved organic carbon and T between 2005 and 2015. The homogeneity of the variances was verified by Levene’s and/or the Brown and Forsythe test.
SMR were carried out with all the lakes sampled each year (14 and 13 lakes in 2005 and 2015, respectively) to quantify the influence of biotic and abiotic factors (Im305, Im320, Im380, ImPAR, total dissolved P, total P, total dissolved N, total N, T, dissolved organic carbon, EOC, TPP, Chl a, Im:total dissolved P, Im: total P, Im: total dissolved N, Im:T, bacterial abundance) on PPP, HBP and %BV in 2005 and 2015. Linearity and multiorthogonality among independent variables were verified by previous correlation analysis and controlled by specifying 0.6 as the minimum acceptable tolerance. Because some factors co-vary with those included by SMR, single regression analyses were performed to assess strength of: i) the C-based control (EOC or dissolved organic carbon) on HBP; ii) the abiotic control (T, UVR, Im305:T, ImPAR:T, Im305:Total P, Im305: total dissolved P) on PPP and %BV; iii) the predatory control (%BV or bacterial abundance) on HBP, to evaluate changes in the algal-bacterial interaction. The normal distribution of residues in all regressions was checked by Kolmogorov-Smirnov tests (Statsoft 1997).
The datasets generated during and/or analysed during the current study are available from the corresponding author (firstname.lastname@example.org).
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The authors are grateful to the members of the Sierra Nevada National Park for their permission and support during the sampling campaign. We are also in debt with Eulogio Corral, Marco J. Cabrerizo, J.A. Delgado-Molina and “members 2005” for their support in the field, and Susana Hitos for their help in the laboratory analysis. We thank David Nesbitt for English-writing assistance. This study was supported by Ministerio Economía y Competitividad and Fondo Europeo de Desarrollo Regional (FEDER) (CGL2011–23681/BOS to P.C., CGL2015–67682-R to P.C. and J.M.M-S.) and Junta de Andalucía (P12-RNM327 to M.V.-A.). J.M.G.O and I.L.L. were supported by the Spanish Government Fellowship “Formación de Profesorado Universitario” (FPU14/00977 and FPU15/05436, respectively).
The authors declare no competing interests.
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González-Olalla, J.M., Medina-Sánchez, J.M., Lozano, I.L. et al. Climate-driven shifts in algal-bacterial interaction of high-mountain lakes in two years spanning a decade. Sci Rep 8, 10278 (2018). https://doi.org/10.1038/s41598-018-28543-2
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