Quiescence is a reversible cell-cycle arrest which allows cancer stem-like cells to evade killing following therapies. Here, we show that proliferating glioblastoma stem-like cells (GSLCs) can be induced and maintained in a quiescent state by lowering the extracellular pH. Through RNAseq analysis we identified Ca2+ signalling genes differentially expressed between proliferating and quiescent GSLCs. Using the bioluminescent Ca2+ reporter EGFP-aequorin we observed that the changes in Ca2+ homeostasis occurring during the switch from proliferation to quiescence are controlled through store-operated channels (SOC) since inhibition of SOC drives proliferating GSLCs to quiescence. We showed that this switch is characterized by an increased capacity of GSLCs’ mitochondria to capture Ca2+ and by a dramatic and reversible change of mitochondrial morphology from a tubular to a donut shape. Our data suggest that the remodelling of the Ca2+ homeostasis and the reshaping of mitochondria might favours quiescent GSLCs’ survival and their aggressiveness in glioblastoma.
Multiform glioblastoma (GBM) is the most aggressive brain tumours with very poor prognosis. Despite a combination of surgical resection, radiotherapy and temozolomide (TMZ)-based chemotherapy, more than 90% of the patients show recurrence and the mean survival period rarely exceeds 2 years1. According to the cancer stem cell model, the GBM lethality is due to a small sub-population of tumour cells with stem-like properties, called Glioblastoma Stem-Like Cells (GSLCs). The GSLCs have been further characterized as slow-cycling or relatively quiescent cells2, identified in vivo in a mouse model of glioblastoma3 and in human glioblastoma tumors4. These quiescent GSLCs are highly resistant to TMZ treatment5.
Quiescence is a cell-cycle arrest state which differs from the one observed in differentiation or senescence by the fact that it is reversible. Transcriptional profiling data reveals that quiescent stem cells are characterized by a common gene signature with the down-regulation of genes associated with cell-cycle progression (i.e. CCNA2, CCNB1 and CCNE2) and the upregulation of genes classified as tumour suppressors, including the cyclin-dependent kinase inhibitor p21 (CDKN1A) and the G0/G1 switch gene 2 (G0S2)6,7. These data also show that quiescence is a G0 phase and not a prolonged G1 phase8. Furthermore, quiescence is actively regulated by signals provided by the stem cell microenvironment. In glioblastoma tumours, quiescent stem-like tumour cells are found close to necrotic tissues, in specific niches characterized by an hypoxic4,5,9 and acidic microenvironment10,11. The role of the microenvironment in the control of GSLCs quiescence is still poorly understood.
Considering that quiescence represents a strategy for GSLCs to evade killing, it is of utmost importance to better characterize the quiescent GSLCs and to understand what governs the transition from a proliferative to a quiescence state. Here, we performed transcriptomic analysis using RNAseq to establish the RNA signatures of proliferative and quiescent GSLCs. We showed that genes involved in Ca2+ signalling are modulated in GSLCs and we explored the causal role of Ca2+ during this transition. Our data points out the reversible remodelling of mitochondrial morphology from tubular to donut shape, associated with an increased capacity of mitochondria to capture Ca2+ and with the modification of the kinetics of Ca2+ influx through SOC. The remodelling of mitochondrial morphology was also observed in an ex-vivo tumour model consisting of large glioblastoma tumorospheres. Our data suggest that the remodelling of the Ca2+ homeostasis and the reshaping of mitochondria during the transition from proliferation to quiescence constitute a protective mechanism that favours survival and aggressiveness of GSLCs.
In vitro induction of quiescence in GSLCs
TG1 and TG1_C1 cells are human GSLCs previously characterized12,13. Previous data showed that TG1 and TG1_C1 cells cultured without medium renewal during 9 days stopped proliferation. This cell-cycle arrest was shown to be reversible, to maintain cells’ stemness and differentiation properties and is not accompanied by cell senescence13. Interestingly, this culture condition induced an acidification of the medium from pH 7.4 to pH 6.6 which correlates with a decrease in EdU incorporation suggesting that the cells adopt a quiescent phenotype14. In order to further characterize this quiescent state, GSLCs were seeded in NS34 medium at pH 7.4 and 6.5 and cell proliferation and viability analysed during 5 days by cell counting and trypan blue exclusion respectively. In proliferating medium (NS34 medium, pH 7.4) the number of TG1 and TG1_C1 cells increased by about 4-fold while at pH 6.5, proliferation rapidly stopped and by day 5 the number of cells was not significantly different from day 0 (Fig. 1A). Analysis of cell viability indicates that lowering extracellular pH (pHe) to 6.5 does not induce cell death (Supplementary Fig. S1). The ability of TG1 cells to form new spheres was evaluated by seeding mechanically dissociated TG1 cells in semi-solid agar medium at pH 7.4 or pH 6.5. Isolated TG1 cells in pH 7.4 medium are able to form spheres of about 40 µm diameter (n = 39.5 µm + 8.8, n = 12), while at pH 6.5, isolated TG1 cells never formed spheres (Fig. 1B). To further confirm that acidic pHe induces proliferation-arrest we measured the number of cells incorporating EdU. The percentage of cells in the S phase decreased drastically in cells kept at pH 6.5 compared to pH 7.4 (at pH 7.4, 39.1% ± 8.9%; at pH 6.5, 4.1% ± 0.8%, p < 0.001, 3 independent experiments), indicating that cells have stopped proliferating (Fig. 1C and Supplementary Fig. S1B). This is confirmed by immunostaining of Ki67 protein (Fig. 1C and Supplementary Fig. S1B), showing that at pH 6.5 TG1 cells had withdrawn from the cell cycle into the G0 phase. Interestingly, the modification of culture conditions from pH 7.4 to pH 6.5 did not alter the expression of the stemness markers, NANOG, OLIG2 and SOX2, known to promote and to maintain stemness of GSLCs15 (Supplementary Fig. S1C). To further demonstrate that the TG1 cells grown at pH 6.5 are in a quiescent state, we analysed the mRNA expression levels of (i) CDKN1A, a cyclin-dependent kinase inhibitor, expressed in quiescent cells and involved in maintenance of the quiescent state16, (ii) CCNB1 (cyclin B1) down-regulated in quiescent cells8 and (iii) G0S2 (G0/G1 Switch 2 gene), encoding a cytosolic protein which promotes quiescence of hematopoietic stem cells7. As expected, the transition from proliferative to quiescent GSLCs is associated with the decrease in the mRNA level for CCNB1 and the increase in mRNA level for CDKN1A and G0S2 (Fig. 1D). Another feature of quiescence is its reversibility6. Quiescent TG1 cells cultured during 5 days at pH 6.5 were transferred to freshly prepared NS34 medium at pH 7.4. Compared to their counterparts kept at pH 6.5, these cells rapidly resumed proliferation as shown by EdU incorporation profile, the number of Ki67 expressing cells, the expression of cell cycle markers and of G0S2 (Supplementary Fig. S2). In order to extend our results to other cell lines, we tested the capacity of BTIC25 GSLCs, from an independent origin17, to be induced toward quiescence. Similarly to TG1 and TG1_C1 cells, BTIC25 cells cultured 5 days in NS34 medium at pH 6.5 stopped proliferating and showed an increased expression of CDKN1A and G0S2 and a decrease in CCNB1 (supplementary Fig. S3). Altogether these results strongly suggest that low pHe activates a cellular program that induces quiescence of GSLCs while preserving stemness properties.
RNA signatures of proliferative and quiescent GSLCs
To characterize the transcriptional changes occurring during quiescence, we performed RNA-sequencing (RNAseq) of TG1 and TG1_C1 cells grown under proliferating conditions and induced to quiescence by either non-renewal of the medium during 9 days or by acidification of pHe to pH 6.5 or pH 6.2 (Supplementary Table S1). RNASeq processed gene data were variance filtered using a threshold of 0.11. A two-group comparison (proliferative versus quiescent cells) was then used to detect genes differentially expressed. We identified a group of 1419 genes using a q-value (the adjusted p-value found using an optimised FDR approach) of 5% to retain the genes with the most significant differential expression (Fig. 2). Principal component analysis (PCA) was used to visualize the variability of our samples. The first axis (PC1) collects 78% of the variance and clearly distinguishes proliferative and quiescent cells, whatever the origin of the samples or the treatment to obtain quiescence (Fig. 2A). Furthermore, the entire set of proliferative samples clustered, pointing to a great similarity between them whatever GSLC clones used (TG1 or TG1_C1). The slightly larger dispersion of the clusters for quiescent cells (Fig. 2A) is certainly related to the difference in the conditions used to reach quiescence. Of note, the group of down-regulated genes in the quiescent samples includes most of the genes belonging to the quiescent gene signature as described for adult stem cells6. Bioinformatics analysis of the gene enrichment for the selected set of genes discloses three main pathways, namely regulation of cell cycle, spindle assembly checkpoint and E2F target genes (Fig. 2B).
Since the remodeling of Ca2+ signaling contributes to cancer hallmarks such as excessive proliferation, survival or resistance to cell death18, we focused on genes belonging to the Ca2+ signaling toolkit19. A set of 105 genes expressed in GSLCs cells were unveiled (Supplementary Table S2). PCA confirmed the clustering of the proliferative GSLCs on the one hand and the quiescent GSLCs on the other hand (Fig. 2C). Using the analysis strategy described above, with the same parameters, a group of 28 genes differentially expressed was further selected (Fig. 2D) suggesting that Ca2+ genes recapitulate the information necessary to distinguish proliferative and quiescent states of the GSLCs. Ca2+ signalling is characterized by its spatiotemporal dynamics resulting from the interplay between Ca2+ fluxes and Ca2+ release and/or uptake by intracellular organelles20 and the mitochondrial Ca2+ uptake capacity contributes also to the shaping of the Ca2+ signal itself21. Importantly, we found that some genes associated with the regulation of Ca2+ influx through plasma membrane Ca2+ channels are up-regulated (CACNB1, CAPS) or down-regulated (CACNA2D1, PKD2, ORAI2) under quiescent conditions. Moreover, the mitochondrial Ca2+ uniporter (MCU), two of its modulators (MICU1 and MICU2) and the voltage-dependent anion channel of the outer mitochondrial membrane (VDAC1), responsible for the transfer of Ca2+ from the endoplasmic reticulum (ER) to the mitochondria were down-regulated in quiescent cells whereas the mitochondrial Na+/Ca2+ exchanger (SLC8B1) was up-regulated (Fig. 2D). This suggests that quiescence is associated with changes in Ca2+ homeostasis through regulation of Ca2+ influx and of mitochondrial Ca2+ uptake.
Remodelling of SOCE activity during the transition from proliferation to quiescence
Store-operated Ca2+ entry (SOCE) is a major mechanism of Ca2+ entry in non-excitable cells. SOCE controls cell proliferation in numerous cell types including cancer cells22,23,24. To examine SOCE between proliferating and quiescent TG1 cells, Ca2+ measurements were performed using EGFP-Aequorin targeted to the cytosol (CytGA) (Supplementary Fig. S4). While in proliferating TG1 cells SOCE is characterized by a transient Ca2+ rise followed by a sustained plateau, quiescent cells displayed a transient Ca2+ increase with a rapid decay and no plateau phase (Fig. 3A). Similar results are obtained with the TG1_C1 cells (Supplementary Fig. S4C). SKF96365 (10 µM), a pharmacological compound known to have inhibitory effects on Ca2+ influx through SOC25, significantly decreased the SOC-mediated Ca2+ transients in both proliferating and quiescent TG1 cells (Fig. 3A). Similar results were obtained with 2-APB (10 µM), another inhibitor of SOCE25. The change in the kinetics of the SOCE decay phase in quiescent GSLCs might reflect modifications in the mechanism of Ca2+ re-uptake in these cells.
Ca2+ is known to control the cell-cycle26,27. TG1 cells were therefore cultured in proliferating medium during 5 days in presence of the membrane-permeant Ca2+ chelator EGTA-AM (5 or 10 µM) to inhibit intracellular Ca2+ increase. Although this treatment blocks proliferation (Fig. S5A), it was not able to induce quiescence as shown by the mRNA level of expression for CDKN1A, CCNB1 and G0S2 (Supplementary Fig. S5B). These results were reinforced by the mRNA expression levels of other cyclins; CCND1, D3, E2 and A2, of PCNA (Proliferating Cell Nuclear Antigen)28 and of Hes1. CCND1 and CCND3 are required during G1, CCNE2 for the G1/S transition and CCNA2 for the S/G2 transition29, Hes1 is a transcription factor involved in the control of the reversibility of cellular quiescence30. Figure S5C clearly indicates that EGTA-treated GSCLs display a mRNA expression profile similar to the one of pH 7.4-cultured GSCLs. In addition, 65.2% + 4.4% (n = 5 independent experiments) of the EGTA-treated TG1 cells still expressed the proliferative marker Ki67 (Fig. S5D). Altogether these data indicate that EGTA-treated GSCLs are arrested in cell-cycle but not in G0. Interestingly, the inhibition of Ca2+ influx through SOC by SKF96365 (10 µM) triggers quiescence of TG1 cells in proliferating medium (Fig. 3B–D and Supplementary Fig. S5E). Indeed, the inhibition of SOC channels with SKF96365 significantly decreased cell proliferation (n = 3, p < 0.0001) and is associated with the up-regulation of CDKN1A and G0S2 and the down-regulation CCNB1 similarly to quiescent GSLCs cultured in acidic pHe medium (Fig. 3B,C). Similar results were obtained with the BTIC25 cells (Fig. S3B,C). The RNAseq analysis confirmed that cells in which Ca2+ influx through SOC was inhibited exhibit a quiescent transcriptomic signature (Fig. 2). Principal component analyses (Fig. 2A,C) showed that SKF96365-treated TG1 cells (experiment #11, Table S1) clearly clustered with the groups of TG1 cells where quiescence was induced by lowering pHe. Altogether, these data indicate that the inhibition of the SOCE not only induces cell-cycle arrest but triggers TG1 cells to adopt a quiescent state and suggests that the transition from proliferation to quiescence involves the remodelling of Ca2+ signalling.
SOCE is mediated by ORAI channels, located at the plasma membrane and STIM1, an ER membrane protein31. Although RNAseq data points to a down-regulation of ORAI2 in all quiescent conditions (Supplementary Table S2), RT-QPCR analysis of the mRNA level for STIM1 and its homologue STIM2 or the ORAI1 and ORAI2 family members showed no significant difference between proliferating and quiescent cells but ORAI3 expression increased significantly in TG1 cells induced to quiescence by lowering pHe to 6.5 (Supplementary Fig. S6). This suggests that the difference in SOCE signals observed might not only be due to differential transcriptional regulations of ORAI and STIM family members.
We hypothesized that the difference in Ca2+ kinetics might be due to the efficiency of Ca2+ recapture. Mitochondria contribute to the regulation of Ca2+ homeostasis by controlling SOCE; this function occurs via mitochondrial Ca2+ uptake32,33. We compared Ca2+ uptake by mitochondria in proliferating and quiescent TG1 and TG1_C1 cells using the bioluminescent Ca2+ reporter EGFP-Aequorin targeted to the mitochondria (MitGA) (Supplementary Fig. S4) and observed that Ca2+ uptake in proliferating and quiescent GSLCs display different kinetics characterized by significantly higher amplitude in quiescent GSLCs (Fig. 4A). Altogether, these data suggest that in quiescent cells, Ca2+ entering through SOC channels is more efficiently recaptured, due to an increased activity of the mitochondrial Ca2+ uptake rather than through modulation of the SOCE mechanism itself.
Mitochondrial dynamics in proliferating and quiescent TG1 cells
In glioma tumours, CSLCs are characterized by fragmented mitochondria relative to mitochondria from differentiated cancer cells34. We compared the mitochondrial morphology of proliferating and quiescent TG1 cells using the TOM20 antibody. Mitochondria of TG1 cells cultured at pH 7.4 display mostly a tubular shape, while a striking change in mitochondrial morphology was observed when TG1 cells are cultured for 5 days at pH 6.5, with the formation of donut-shaped mitochondria. Donut-shaped mitochondria were identifiable as circular structures with a central hole and a diameter of >0.8 µm (Fig. 4B). Indeed, while only 9% (n = 390, 4 independent experiments) of the proliferating TG1 cells have donut-shaped mitochondria, this proportion is increased in quiescent TG1 cells with 68% (n = 389, 4 independent experiments) showing donut-shaped mitochondria (Fig. 4C). This quiescence linked transition from tubular to donut- shaped mitochondria is also observed for BITC25 cells (Fig. S7). Interestingly, the reshaping of mitochondria is reversible, the percentage of donut-shaped mitochondria decreased when quiescent TG1 cells are transferred back to pH 7.4 (Fig. 4C). Since SKF96365-treated TG1 cells are quiescent, we next examined whether it is associated with the modification of mitochondrial morphology. 93% of the SKF96365-treated TG1 cells (n = 100, 3 independent experiments) have donut-shaped mitochondria (Fig. 4C). These observations suggest that donut-shaped mitochondria might be a characteristic feature of quiescent GSLCs.
Mitochondrial morphology remodelling in glioblastoma tumorospheres
In vitro, GSLCs can form large floating spheres of more than 500 µm diameter, called macro-tumorospheres35 which present strong similarities in their cellular organization with the 3D organoid culture systems or glioblastoma tumours in vivo5,36. We previously showed that TG1 or TG1_C1 GSLCs form such macro-tumorospheres characterized by cellular heterogeneity in terms of structure and cell composition with proliferating Ki67 positive cells localized to the outer rim while slow-cycling cells are found in the central hypoxic, necrotic and probably acidic core14. These macro-tumorospheres constitute a favourable in vitro assay to compare mitochondria morphology. To this end, mitochondria were labelled with the TOM20 antibody (Fig. 5Aa,b). Quantification as detailed in the method section, revealed significantly higher number of cells with donut-shaped mitochondria (77%, 3 independent experiments) in the core as compared to the periphery (7%, 3 independent experiments) (Fig. 5B). These data confirm the observation obtained with isolated TG1 cells and show that within the macro-tumorospheres, the mitochondrial morphology undergoes remodelling according to the proliferating status of the GSLCs.
Glioblastoma tumour recurrence has been attributed to the GSLCs which reside within the tumour mass in hypoxic and acidic microenvironments in a slow-cycling or quiescent state. However, standard in vitro culture conditions which favour proliferating GSLCs over quiescent ones fail to reproduce the tumour conditions and preclude studies on the mechanisms controlling quiescence. We established that modification of the culture condition by lowering pHe from pH 7.4 to pH 6.5 induces cell-cycle arrest in GSLCs as confirmed by the up-regulation of the expression of CDKN1A and G0S2 and the down-regulation of CCNB1, without modification of their stemness properties as seen by the expression of the stemness markers, NANOG, OLIG2 and SOX2,. This cell-cycle arrest is reversible; GSLCs transferred back to proliferating culture condition at pH 7.4 rapidly resumed proliferation. Altogether these data indicate that the acidic treatment of GSLCs induces a quiescent G0 state.
This simple culture protocol allowed us to compare the transcriptomic profile of proliferating and quiescent GSLCs and to perform Ca2+ imaging to directly test their respective Ca2+ responses. Accumulating evidences suggest that Ca2+ might be an important regulator of tumorigenesis in GBM37,38. We found that in GSLCs maintained in acidic medium, genes associated with cell-cycle progression are down-regulated and that genes coding proteins involved in Ca2+ signalling, particularly plasma membrane and organelles Ca2+ channels, are modulated. We showed that the inhibition of SOC channels by SKF96365 not only blocks cell proliferation but was sufficient to lead GSLCs toward a quiescent state. This suggests that Ca2+ signalling through SOC channels is necessary to regulate the balance proliferation/quiescence. To the best of our knowledge, this is the first time that a causal role can be ascribed to Ca2+ signalling in the transition of cells from proliferation to quiescence. How is Ca2+ homeostasis regulated in this process? STIM1 and ORAI1 proteins have been identified as critical components for Ca2+ entry through SOC however, increasing evidence showed that alteration of STIM1 and ORAI1 but also of ORAI3 expression and function contribute to tumorigenesis38,39. Using aequorin-based Ca2+ recordings to follow intracellular Ca2+ fluxes, we observed that GSLCs cultured in stem-cell medium under proliferative or quiescent conditions have distinct kinetics of Ca2+ entry through SOC channels. This difference in kinetics could be due to differential expression of the genes coding for the STIM1/2 and/or ORAI1-3 family members. Here we showed that only the expression of ORAI3 is modified between proliferating and quiescent GSLCs. Careful examination of the SOC kinetics revealed that the initial rate of Ca2+ influx is not modified in quiescent GSLCs but that the prolonged plateau is markedly attenuated. This difference in the SOC Ca2+ kinetics might rather be due to an increased efficiency of intracellular Ca2+ re-capture by quiescent GSLCs.
Mitochondria are crucial organelles which not only regulate cellular energy generation and apoptosis but also control intracellular Ca2+ signalling particularly by their capacity to take up Ca2+ thereby modulating SOC channels40. This capacity of mitochondria to actively buffer cytosolic Ca2+ occurs via the mitochondrial Ca2+ uniporter MCU associated with regulatory subunits including MICU1, MICU2 and EMRE41. Our data on Ca2+ homeostasis with the bioluminescent probe Aequorin support this view. We observed that the stimulation of Ca2+ entry via SOC channels by depletion of the internal ER stores triggered a large mitochondrial Ca2+ transients in GSLCs induced to quiescent by lowering pHe to 6.5 compared to proliferative GSLCs at pH 7.4. While these results would suggest that the activity of MCU is enhanced in quiescent GSLCs, our RNAseq data point to a reduced expression of MCU and of two of its regulators, MICU1 and MICU2 in quiescent GSLCs compared to proliferative GSLCs. It has been shown in the literature that the rate of Ca2+ entry through MCU depends on the cytosolic Ca2+ concentrations ([Ca2+]cyt), with slow rate for low [Ca2+]cyt and faster rate when the [Ca2+]cyt increases. This sigmoidal behaviour has been mainly attributed to the functions of MICU1 and MICU2. Although their respective gatekeeping functions are still debated, experimental evidences clearly indicate that MICU2 silencing induces an increase of mitochondrial Ca2+ uptake, indicating that MICU2 is an inhibitor of MCU. On the contrary, MICU1 behaves as a Ca2+-sensitive switch for MCU activity; acting as inhibitor of MCU at low [Ca2+]cyt and activator at high [Ca2+]cyt42. We observed that the resting level of cytosolic Ca2+ is lower in proliferating TG1 cells compared to quiescent ones. In proliferating TG1 cells, MICU1 and MICU2 should therefore be inhibitors of MCU and its activity should be low. However, in quiescent TG1 cells we found that MICU2 expression was reduced and as a consequence, its inhibitory effect on MCU released. Although MCU expression is reduced in quiescent cells, MCU activity would be enhanced. Our RNAseq data also pointed to the overexpression, in quiescent cells, of the Na/Ca2+ exchanger SLC8B1, recently identified as one of the components responsible for mitochondrial Ca2+ efflux and which counteracted the accumulation of Ca2+ into mitochondrial matrix by MCU43. Therefore, the transcriptional remodelling of components that control mitochondrial Ca2+ homeostasis may contribute to prevent mitochondrial Ca2+ overload during quiescence.
It has been reported that mitochondria are highly dynamic organelles whose morphology is controlled by fission-fusion mechanisms. Under normal conditions mitochondria are essentially tubular but can be fragmented during apoptosis or formed distended swollen structures during necrosis44. Besides these tubular or fragmented shapes, a third shape called donut was reported during hypoxia-reoxygenation stress45. Donut-shaped mitochondria result from the bending and the fusion of the two ends of tubular mitochondria46. We observed that proliferative GSLCs have mainly tubular mitochondria, while quiescent GSLCs mainly show donut-shaped mitochondria. This reshaping of mitochondria is reversible. When GSLCs are transferred from a quiescent to a proliferative medium donut-shaped mitochondria switch back to a tubular shape. Recent studies on the relationship between mitochondrial shapes and stress show that the transition from tubular to donut requires an increase of mitochondrial Ca2+ uptake47 and does not involve fusion mechanism45. Our results are in accordance with the requirement of enhanced mitochondrial Ca2+ uptake and the formation of donut-shaped mitochondria. This remodelling of mitochondrial morphology from tubular to donut may be an additional mechanism explaining the increased mitochondrial Ca2+ uptake capacity observed after SOC stimulation in quiescent GSLCs. An interesting observation that may contribute to the formation of donut-shaped mitochondria during quiescence is the down-regulation of the LETM1 gene (see RNAseq data). LETM1 is a leucine zipper and EF-hand transmembrane protein localized to the inner mitochondrial membrane48 which has been shown to be required to maintain the mitochondrial tubular network independently of the mechanism of mitochondrial fission which involved the DRP1-dependent pathway49.
Mitochondrial dysfunction is one of the hallmarks of cancers and this is associated with cancer cells exhibiting fragmented mitochondria50. On large macro-tumorospheres, we found that a large fraction of mitochondria forms a tubular network in proliferating GSLCs while GSLCs localized to the core of the tumorosphere are characterized by donut-shaped mitochondria. Importantly, the presence of a hypoxic environment has been documented within the core of these macro-tumorospheres14. As suggested by45, the formation of donut-shaped mitochondria might represent a protective mechanism preserving mitochondria against autophagy upon metabolic stress.
In conclusion, our findings on mitochondria in GSLCs point out the regulation of mitochondria dynamics and ER network. The relationship between changes in mitochondrial shape and physiological parameters, particularly mitochondrial Ca2+, has therefore a great potential for translational research. Mitochondria targeted therapeutic may involve the control of the shape correlated with Ca2+ signalling.
Cells and growth conditions
GSLCs (TG1, TG1_C1 and BITC25) lines were isolated, established and cultures from surgical resections of primary GBMs from adult patients (age over 18) after informed consent as previously described12,17,51. These cells express stem cells markers, have clonal properties, were able to differentiate and induce tumours when orthotopically grafted in immune-compromised mice12,52. Their mutational status is however different, while BITC25 is mutated for TP53, TG1 and TG1_C1 are not12,52. The cells are expanded in NS34 serum-free medium containing EGF and bFGF (DMEM-F12 1/1, glutamine 10 mM, Hepes 10 mM, Sodium bicarbonate 0.025%, N2, G5, and B27) as described53 and used up to 10 passages. Under the culture conditions used, TG1, TG_C1 and BTIC25 cell grew as floating neurospheres. To induce quiescence, GSLCs were either cultured 9 days without medium removal as described13 or 5 days in NS34 medium buffered to pH 6.2 or 6.5. Macro-tumorospheres from single GSLCs were obtained as described in14.
Cell proliferation, viability and EdU assay
Cells were seeded at 1∙105cells/ml in 6-well plate and proliferation assessed by direct cell counting method using Neubauer Improved C-C-Chip. Cell viability was evaluated using Trypan blue exclusion assay. The number of cells in S phase was determined with Click-it® Edu Alexa fluor 647 Imaging Kit (Invitrogen) as indicated. At day 5, cells were incubated with 10 µM EdU solution during 2 hours, fixed in 16% paraformaldehyde (PFA) for 30 minutes, transferred on SuperFrost slides (Thermo Scientific, France) and observed with Leica Sp8 confocal microscope.
Sphere formation assay and formation of clonal tumorospheres from single GSLCs in vitro
To assess the ability of TG1 cells to form new clones, spheres were mechanically dissociated into single cell suspension and seeded into 24-wells plates at 104 cells per well in soft-agar (0.7% agarose) NS34 medium at pH 7.4 or pH 6.5. This allows to continuously follow sphere formation from individual isolated TG1 cells during 7 days. Pictures were taken with a Nikon Eclipse TS100 Microscope, with 40X/0.55 and 20X/0.40 objectives from day 1 to day 7. Macro-tumorospheres from single GSLCs were obtained as described in14.
Relative expression levels of several genes (CDKN1A, CCNB1, GOS2, CCND1, CCND3, CCNE2, CCNA2, PCNA, Hes1, STIM1, 2, ORAI1, 2, 3, MICU1, 2 and MCU) relative to TBP (TATA-Box Binding Protein), in proliferating and quiescent TG1 cells were assessed by real-time qRT–PCR. Total RNA was extracted with RNeasy® mini kit (Qiagen) and quantified in a NanoDrop apparatus. RNA (0.4 μg) was reverse transcribed using q-ScriptTM cDNA SuperMix (Quanta, Biosciences). Amplifications of 8 ng of cDNA were performed in triplicate using Eva-Green® SuperMix (Bio Rad) in a 10 μl reaction mixture containing 500 nM primers (S4 Table), run on BioRad CFX96 thermocycler.
RNAseq data and analysis
To establish RNA signatures of proliferative and quiescent GSLCs, the following strategy was used. Several experiments were performed in order to take into account different types of variabilities (Supplementary Table S1); (i). To address variability due to laboratories environments, experiments were done in two laboratories (Strasbourg and Toulouse) following identical protocols. The cells were obtained from the same master cell bank and the composition of the growing media was identical. (ii) For cellular variability, two cell lines TG1 and TG1_C1 were used. (iii) for variability in inducing quiescence, the switch to the quiescent state was obtained by either the non-replacement of the medium during 9 days, or acidification of the medium to pH 6.5 or 6.2 for 5 days or treatment of the cells by 10 µM SKF96365 in the growing medium (pH 7.4) at day 1 and 3 and analysis at day 5. In normal medium at pH 7.4, the cells are in a proliferative state. Total RNA was extracted as described above and RNA quality was controlled with AATI Fragment Analyser (Advanced Analytical Technologies, Inc). RNA-seq was performed at the Strasbourg IGBMC platform. Short reads were aligned using the reference genome hg38 (http://genomeast.igbmc.fr). The Qlucore software (htpp://www.glucore.org) used for the analysis. Reads (fastq files) were mapped onto the hg38 assembly of the Homo sapiens genome using Tophat 2.0.1054 and bowtie version 2-2.1.055 to give BAM files. From the BAM files and the hg38 GTF file, Qlucore Omics Explorer creates a count matrix by counting the number of reads overlapping each genomic feature in each sample (each BAM file). The counts are then normalized across samples and across genes, and finally log2-transformed. The processed data have also been deposited in the GEO datasets database under the accession number GSE 93991.
Proliferating or quiescent TG1 cells were cultured in suspension for 5 days at pH 7.4 and pH 6.5 respectively and processed as described14. Primary antibodies used in this study are listed in Supplementary Table S3.
Analysis of mitochondrial morphology in clonal macro-tumorospheres
Sections of clonal macro-tumorospheres were obtained as described14. Mitochondria were labelled with an antibody against TOM20. For each section, 5 randomly selected fields were taken in the periphery and in the core. Cells containing at least one donut-shaped mitochondrion were counted as positive. Donut-shaped mitochondria were manually counted considering the structures with a central hole and a donut diameter >0.8 µm46.
The chimeric GFP-aequorin (GA) targeted to the cytosol (CytGA) is a generous gift from Ph. Brûlet56. We constructed the chimeric GA targeted to mitochondria (MitGA). The starting sequence of GA was obtained by PCR from CytGA construct with primers containing a BamHI site at the 5′ end (CCggatccAGCAAGGGCGAGGAGCTGTTC) and XbaI site at the 3′ end (CCtctagaTTAGGGGACAGCTCCACCG). The mitochondrial targeting sequence was fused in frame at the 5′ end with a 86 bp sequence of the human cytochrome c oxidase VIII subunit (COX VIII) isolated from pDsRed2-Mito by digestion with BamHI and XbaI from pDsred2-Mito.
Ca2+ measurements using aequorin probes
Cells (2∙106) were electroporated with 2 µg of DNA encoding either the Mitochondrial-targeted EGFP-Aequorin (MitGA) or Cytosolic-targeted EGFP-Aequorin (CytGA) probes, using AMAXA Kit L cell line nucleofactor (Lonza). The localization of the Ca2+ reporter to mitochondria was confirmed using an antibody directed against a mitochondrial protein, TOM20, a 20 kDa translocase of outer mitochondrial membranes. Mitochondrial or cytosolic Ca2+ measurements were performed at 37 °C and the temporal aequorin-generated data were acquired as described14. Ca2+ measurements were performed on isolated TG1 cells immobilized in agar. Ca2+ influx through SOCE was obtained by challenging the SOC channels through depletion, in Ca2+-free medium, of ER store with thapsigargin (1 µM), an inhibitor of the ER Ca2+-ATPase. Following this treatment, bath application of 1 mM Ca2+ restores Ca2+ influx through SOC57.
The results are expressed as means + S.D. The statistical significance was evaluated using the Student’s t-test or one-way ANOVA with GraphPad Prism6 software. *p < 0.05–0.001, ****p < 0.0001
All sequencing data are available from the GEO database (accession number: GSE 93991).
Stupp, R. et al. Radiotherapy plus concomitant and adjuvant temozolomide for glioblastoma. N Engl J Med 352, 987–996, https://doi.org/10.1056/NEJMoa043330 (2005).
Deleyrolle, L. P. et al. Evidence for label-retaining tumour-initiating cells in human glioblastoma. Brain 134, 1331–1343, https://doi.org/10.1093/brain/awr081 (2011).
Chen, J. et al. A restricted cell population propagates glioblastoma growth after chemotherapy. Nature 488, 522–526, https://doi.org/10.1038/nature11287 (2012).
Ishii, A. et al. Histological Characterization of the Tumorigenic “Peri-Necrotic Niche” Harboring Quiescent Stem-Like Tumor Cells in Glioblastoma. PLoS One 11, e0147366, https://doi.org/10.1371/journal.pone.0147366 (2016).
Pistollato, F. et al. Intratumoral hypoxic gradient drives stem cells distribution and MGMT expression in glioblastoma. Stem Cells 28, 851–862, https://doi.org/10.1002/stem.415 (2010).
Cheung, T. H. & Rando, T. A. Molecular regulation of stem cell quiescence. Nat Rev Mol Cell Biol 14, 329–340, https://doi.org/10.1038/nrm3591 (2013).
Yamada, T., Park, C. S., Burns, A., Nakada, D. & Lacorazza, H. D. The cytosolic protein G0S2 maintains quiescence in hematopoietic stem cells. PLoS One 7, e38280, https://doi.org/10.1371/journal.pone.0038280 (2012).
Coller, H. A., Sang, L. & Roberts, J. M. A new description of cellular quiescence. PLoS Biol 4, e83, https://doi.org/10.1371/journal.pbio.0040083 (2006).
Persano, L., Rampazzo, E., Della Puppa, A., Pistollato, F. & Basso, G. The three-layer concentric model of glioblastoma: cancer stem cells, microenvironmental regulation, and therapeutic implications. ScientificWorldJournal 11, 1829–1841, https://doi.org/10.1100/2011/736480 (2011).
Garcia-Martin, M. L. et al. High resolution pH(e) imaging of rat glioma using pH-dependent relaxivity. Magn Reson Med 55, 309–315, https://doi.org/10.1002/mrm.20773 (2006).
Honasoge, A., Shelton, K. A. & Sontheimer, H. Autocrine regulation of glioma cell proliferation via pHe-sensitive K(+) channels. Am J Physiol Cell Physiol 306, C493–505, https://doi.org/10.1152/ajpcell.00097.2013 (2014).
Patru, C. et al. CD133, CD15/SSEA-1, CD34 or side populations do not resume tumor-initiating properties of long-term cultured cancer stem cells from human malignant glio-neuronal tumors. BMC Cancer 10, 66, https://doi.org/10.1186/1471-2407-10-66 (2010).
Zeniou, M. et al. Chemical Library Screening and Structure-Function Relationship Studies Identify Bisacodyl as a Potent and Selective Cytotoxic Agent Towards Quiescent Human Glioblastoma Tumor Stem-Like Cells. PLoS One 10, e0134793, https://doi.org/10.1371/journal.pone.0134793 (2015).
Dong, J. et al. Bisacodyl and its cytotoxic activity on human glioblastoma stem-like cells. Implication of inositol 1,4,5-triphosphate receptor dependent calcium signaling. Biochim Biophys Acta, https://doi.org/10.1016/j.bbamcr.2017.01.010 (2017).
Suvà, M. L. et al. Reconstructing and reprogramming the tumor-propagating potential of glioblastoma stem-like cells. Cell 157, 580–594, https://doi.org/10.1016/j.cell.2014.02.030 (2014).
Cheng, T. et al. Hematopoietic stem cell quiescence maintained by p21cip1/waf1. Science 287, 1804–1808 (2000).
Kelly, J. J. et al. Proliferation of human glioblastoma stem cells occurs independently of exogenous mitogens. Stem Cells 27, 1722–1733, https://doi.org/10.1002/stem.98 (2009).
Prevarskaya, N., Ouadid-Ahidouch, H., Skryma, R. & Shuba, Y. Remodelling of Ca2+ transport in cancer: how it contributes to cancer hallmarks? Philos Trans R Soc Lond B Biol Sci 369, 20130097, https://doi.org/10.1098/rstb.2013.0097 (2014).
Robil, N., Petel, F., Kilhoffer, M.-C. & Haiech, J. Glioblastoma and calcium signaling - analysis of calcium toolbox expression. Int. J. Dev. Biol. 59, 407–415, https://doi.org/10.1387/ijdb.150200jh (2015).
Berridge, M. J., Lipp, P. & Bootman, M. D. The versatility and universality of calcium signalling. Nat Rev Mol Cell Biol 1, 11–21, https://doi.org/10.1038/35036035 (2000).
Giorgi, C. et al. Mitochondrial calcium homeostasis as potential target for mitochondrial medicine. Mitochondrion 12, 77–85, https://doi.org/10.1016/j.mito.2011.07.004 (2012).
Che, H. et al. Roles of store-operated Ca2+ channels in regulating cell cycling and migration of human cardiac c-kit+ progenitor cells. Am J Physiol Heart Circ Physiol 309, H1772–1781, https://doi.org/10.1152/ajpheart.00260.2015 (2015).
El Boustany, C. et al. Differential roles of STIM1, STIM2 and Orai1 in the control of cell proliferation and SOCE amplitude in HEK293 cells. Cell Calcium 47, 350–359, https://doi.org/10.1016/j.ceca.2010.01.006 (2010).
Li, G. et al. Suppression of STIM1 inhibits human glioblastoma cell proliferation and induces G0/G1 phase arrest. J Exp Clin Cancer Res 32, 20, https://doi.org/10.1186/1756-9966-32-20 (2013).
Várnai, P., Hunyady, L. & Balla, T. STIM and Orai: the long-awaited constituents of store-operated calcium entry. Trends Pharmacol Sci 30, 118–128, https://doi.org/10.1016/j.tips.2008.11.005 (2009).
Moreau, M., Guerrier, P., Doree, M. & Ashley, C. C. Hormone-induced release of intracellular Ca2+ triggers meiosis in starfish oocytes. Nature 272, 251–253 (1978).
Humeau, J. et al. Calcium signaling and cell cycle: Progression or death. Cell Calcium 70, 3–15, https://doi.org/10.1016/j.ceca.2017.07.006 (2018).
Tan, C. K., Castillo, C., So, A. G. & Downey, K. M. An auxiliary protein for DNA polymerase-delta from fetal calf thymus. J Biol Chem 261, 12310–12316, https://doi.org/10.1111/jth.13253 (1986).
Hochegger, H., Takeda, S. & Hunt, T. Cyclin-dependent kinases and cell-cycle transitions: does one fit all? Nat Rev Mol Cell Biol 9, 910–916, https://doi.org/10.1038/nrm2510 (2008).
Sang, L., Coller, H. A. & Roberts, J. M. Control of the reversibility of cellular quiescence by the transcriptional repressor HES1. Science 321, 1095–1100, https://doi.org/10.1126/science.1155998 (2008).
Kraft, R. S. T. I. M. and ORAI proteins in the nervous system. Channels (Austin) 9, 245–252, https://doi.org/10.1080/19336950.2015.1071747 (2015).
Demaurex, N., Poburko, D. & Frieden, M. Regulation of plasma membrane calcium fluxes by mitochondria. Biochim Biophys Acta 1787, 1383–1394, https://doi.org/10.1016/j.bbabio.2008.12.012 (2009).
Rizzuto, R., De Stefani, D., Raffaello, A. & Mammucari, C. Mitochondria as sensors and regulators of calcium signalling. Nat Rev Mol Cell Biol 13, 566–578, https://doi.org/10.1038/nrm3412 (2012).
Xie, Q. et al. Mitochondrial control by DRP1 in brain tumor initiating cells. Nat Neurosci 18, 501–510, https://doi.org/10.1038/nn.3960 (2015).
Weiswald, L. B., Bellet, D. & Dangles-Marie, V. Spherical cancer models in tumor biology. Neoplasia 17, 1–15, https://doi.org/10.1016/j.neo.2014.12.004 (2015).
Hubert, C. G. et al. A Three-Dimensional Organoid Culture System Derived from Human Glioblastomas Recapitulates the Hypoxic Gradients and Cancer Stem Cell Heterogeneity of Tumors Found In Vivo. Cancer Res 76, 2465–2477, https://doi.org/10.1158/0008-5472.CAN-15-2402 (2016).
Leclerc, C. et al. Calcium signaling orchestrates glioblastoma development: Facts and conjunctures. Biochim Biophys Acta 1863, 1447–1459, https://doi.org/10.1016/j.bbamcr.2016.01.018 (2016).
Fiorio Pla, A., Kondratska, K. & Prevarskaya, N. STIM and ORAI proteins: crucial roles in hallmarks of cancer. Am J Physiol Cell Physiol 310, C509–519, https://doi.org/10.1152/ajpcell.00364.2015 (2016).
Liu, H., Hughes, J. D., Rollins, S., Chen, B. & Perkins, E. Calcium entry via ORAI1 regulates glioblastoma cell proliferation and apoptosis. Exp Mol Pathol 91, 753–760, https://doi.org/10.1016/j.yexmp.2011.09.005 (2011).
Samanta, K., Douglas, S. & Parekh, A. B. Mitochondrial calcium uniporter MCU supports cytoplasmic Ca2+ oscillations, store-operated Ca2+ entry and Ca2+ -dependent gene expression in response to receptor stimulation. PLoS One 9, e101188, https://doi.org/10.1371/journal.pone.0101188 (2014).
Patron, M. et al. MICU1 and MICU2 finely tune the mitochondrial Ca2+ uniporter by exerting opposite effects on MCU activity. Mol Cell 53, 726–737, https://doi.org/10.1016/j.molcel.2014.01.013 (2014).
Csordás, G. et al. MICU1 controls both the threshold and cooperative activation of the mitochondrial Ca²+ uniporter. Cell Metab 17, 976–987, https://doi.org/10.1016/j.cmet.2013.04.020 (2013).
Palty, R. et al. NCLX is an essential component of mitochondrial Na+/Ca2+ exchange. Proc Natl Acad Sci USA 107, 436–441, https://doi.org/10.1073/pnas.0908099107 (2010).
Westermann, B. Bioenergetic role of mitochondrial fusion and fission. Biochim Biophys Acta 1817, 1833–1838, https://doi.org/10.1016/j.bbabio.2012.02.033 (2012).
Liu, X. & Hajnóczky, G. Altered fusion dynamics underlie unique morphological changes in mitochondria during hypoxia-reoxygenation stress. Cell Death Differ 18, 1561–1572, https://doi.org/10.1038/cdd.2011.13 (2011).
Long, Q. et al. Modeling of Mitochondrial Donut Formation. Biophys J 109, 892–899, https://doi.org/10.1016/j.bpj.2015.07.039 (2015).
Ahmad, T. et al. Computational classification of mitochondrial shapes reflects stress and redox state. Cell Death Dis 4, e461, https://doi.org/10.1038/cddis.2012.213 (2013).
Jiang, D., Zhao, L. & Clapham, D. E. Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. Science 326, 144–147, https://doi.org/10.1126/science.1175145 (2009).
Dimmer, K. S. et al. LETM1, deleted in Wolf-Hirschhorn syndrome is required for normal mitochondrial morphology and cellular viability. Hum Mol Genet 17, 201–214, https://doi.org/10.1093/hmg/ddm297 (2008).
Senft, D. & Ronai, Z. A. Regulators of mitochondrial dynamics in cancer. Curr Opin Cell Biol 39, 43–52, https://doi.org/10.1016/j.ceb.2016.02.001 (2016).
Silvestre, D. C. et al. Alternative lengthening of telomeres in human glioma stem cells. Stem Cells 29, 440–451, https://doi.org/10.1002/stem.600 (2011).
Cusulin, C. et al. Precursor States of Brain Tumor Initiating Cell Lines Are Predictive of Survival in Xenografts and Associated with Glioblastoma Subtypes. Stem Cell Reports 5, 1–9, https://doi.org/10.1016/j.stemcr.2015.05.010 (2015).
Fève, M. et al. Comparative expression study of the endo-G protein coupled receptor (GPCR) repertoire in human glioblastoma cancer stem-like cells, U87-MG cells and non malignant cells of neural origin unveils new potential therapeutic targets. PLoS One 9, e91519, https://doi.org/10.1371/journal.pone.0091519 (2014).
Kim, D. et al. TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions. Genome Biol 14, R36, https://doi.org/10.1186/gb-2013-14-4-r36 (2013).
Langmead, B. & Salzberg, S. L. Fast gapped-read alignment with Bowtie 2. Nat Methods 9, 357–359, https://doi.org/10.1038/nmeth.1923 (2012).
Baubet, V. et al. Chimeric green fluorescent protein-aequorin as bioluminescent Ca2+ reporters at the single-cell level. Proc Natl Acad Sci USA 97, 7260–7265 (2000).
Bird, G. S., DeHaven, W. I., Smyth, J. T. & Putney, J. W. Methods for studying store-operated calcium entry. Methods 46, 204–212, https://doi.org/10.1016/j.ymeth.2008.09.009 (2008).
We thank Drs H. Chneiweiss, M-P. Junier for fruitful discussions, A. Davy for providing the BITC25 cells, P. Bellenger for the anti-TOM20 antibody, P. Cochard for the anti-Olig2 antibody and for advice in microscopy imaging and the LITC-CBD Imaging platform and B. Ronsin and S. Bosch for microscopy assistance. Sequencing was performed by the IGBMC Microarray and Sequencing platform, a member of the ‘France Génomique’ consortium (ANR-10-INBS-0009). This work was supported by the Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg, Université Toulouse 3, by a joint grant from the Agence Nationale de la Recherche (ANR) given between France and Hong Kong to C.L., J.H., and M.M. (CalciumGlioStem ANR-13-ISV1-0004 and A-HKUST601/13), SATT Conectus (M-C.K), and has been performed within the LABEX ANR-10-LABX-0034_Medalis and received a financial support from French government managed by “Agence Nationale de la Recherche” under “Programme d’investissement d’avenir”. F.J.A. and J.D. were supported by a grant from the ANR CalciumGlioStem. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
The authors declare no competing interests.
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Aulestia, F.J., Néant, I., Dong, J. et al. Quiescence status of glioblastoma stem-like cells involves remodelling of Ca2+ signalling and mitochondrial shape. Sci Rep 8, 9731 (2018). https://doi.org/10.1038/s41598-018-28157-8
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