# A Predictive Spatial Distribution Framework for Filovirus-Infected Bats

## Abstract

Tools with predictive capabilities in regards of filovirus outbreaks are mainly anthropocentric and have disregarded the ecological dimension of the problem. Here we contribute to shift the current paradigm by studying the dynamics of the putative main zoonotic niche of filoviruses, bats, and its link to environmental drivers. We propose a framework that combines data analysis, modeling, and the evaluation of sources of variability. We implement a regression analysis using factual data to correlate environmental parameters and the presence of bats to find the distribution of resources. The information inferred by the regression is fed into a compartmental model that describes the infection state. We also account for the lack of knowledge of some parameters using a sampling/averaging technique. As a result we estimate the spatio-temporal densities of bats. Importantly, we show that our approach is able to predict where and when an outbreak is likely to appear when tested against recent epidemic data in the context of Ebola. Our framework highlights the importance of considering the feedback between the ecology and the environment in zoonotic models and sheds light on the mechanisms to propagate filoviruses geographically. We expect that our methodology can help to design prevention policies and be used as a predictive tool in the context of zoonotic diseases associated to filoviruses.

## Introduction

A large percentage of emerging infectious diseases affecting humans have an animal origin: zoonoses. In particular the Filoviridae family causes severe hemorrhagic fevers, e.g. Ebola, in human and nonhuman primates. Ebola is indeed among the zoonotic diseases with highest mortality rate, up to 90%1: the 2014 epidemic in West Africa has been the largest registered ever, with around 28,000 probable human cases and a 69% fatality rate2. Therefore, understanding the factors and the mechanisms that lead to outbreaks associated with filoviruses is extremely important.

The Ebolavirus (EBOV) strain found during the 2014 outbreak in West Africa was identified as Zaire’s3. This suggests the importance of mobility factors of wildlife vectors in zoonoses4, even if there are no definitive proofs on the reservoir origin of the outbreak. The World Heath Organization concluded that, prior to the onset, the child that originated the epidemic was seen playing in his backyard near a tree heavily infested with bats and pointed to the exposure of a human to bats as the cause. However, no epidemiologic or genetic data associate this putative reservoir species with the 2014 outbreak5. Nonetheless, several other studies have linked filoviruses to bats ecology. Leroy et al. reported in a seminal study that anti-EBOV antibodies and EBOV RNA were detected in fruit bat species6. Further studies have confirmed this finding7,8. In addition, there is evidence of Ebola outbreaks due to a direct exposure of humans to bats in Africa9. In this context, the difficulties for identifying the main zoonotic reservoir is partly due to the large diversity of EBOV hosts1. In fact, even if bats were indeed the main and unique reservoir of filoviruses as EBOV, most outbreaks might not be primarily driven by direct human-bat contacts, but by other means, such as infected primate carcasses (bushmeat), direct contact with other infected hosts, or contact with bat feces. Recent studies have carefully reexamined the evidence in favor and against bats as the reservoir of filoviruses including EBOV5,10,11,12. Regardless of the controversy in the field and the acknowledgment that Ebola and other filovirus diseases are a multifactorial problem, bats have been identified as an important (direct and/or indirect) driver of outbreaks of these diseases. The fact that bats are able to clear filovirus infections13 and travel thousands of kilometers per year in search of better environmental conditions (e.g.,food, shelter)14,15 raises the question of how their infection and migration dynamics shape the zoonotic niche of filoviruses. In this study we aim at addressing that question using a novel modeling framework.

During the past years the modeling efforts in the context of filoviruses have been intense16,17,18,19,20. In particular, a large number of mathematical models have been proposed to understand the West Africa EBOV infection dynamics from different perspectives, including epidemic, genomic, and social data16,17,18,19,20,21,22,23. These models have been shown to provide estimates of the progression of the disease in the short-term (weeks) once an outbreak occurs. However, besides the success of these approaches in describing the dynamics of the infection in human populations, only recently there has been an effort to link the appearance of outbreaks using a zoonotic perspective24,25,26,27,28. In this regard, we hypothesize that the infection dynamics of bats can be correlated with filovirus outbreaks. To that end, we introduce here a predictive framework that integrates data and modeling to study the infection dynamics of bats driven by enviroclimatic factors.

Our framework combines a statistical regression of factual data to estimate the dependence of the carrying capacity on environmental parameters, a sampling technique to account for the variability of parameters, and a compartmental model that describes the filovirus infection dynamics in chiroptera species28. Notably, when comparing our results with the 2014 Ebola epidemic data, we show that our approach is able to pinpoint geographical locations where, and time points when, EBOV infected bats emerged. This in turn may explain the suggested link between resources in a region and the onset of the Ebola epidemic in West Africa4. Therefore, we expect that our methodology can be used to design prevention policies and as an additional resource for reducing the risk of future spillovers.

## Results

### Data Regression Analysis Reveals Key Environmental Parameters in the Ecology of the Zoonotic Niche

In order to clarify what key environmental parameters drive the ecology of the zoonotic niche, we implemented a regression approach to correlate environmental factors relevant to bats ecology with their population at specific locations (see Methods). Environmental/climate data was retrieved using the Google Earth Engine tool to access the databases from NASA Land Processes Distributed Active Archive Center (LP DAAC), USGS/Earth Resources Observation and Science (EROS) Center, Sioux Falls, South Dakota. For data about bat colonies at specific locations we used the data collected by Bergmans29. In order to improve the statistics of our approach, we also included expected presence and absence data about bats using the IUCN database30. We notice that although there is some data available about bats obtained from serological surveillance studies indicating the infectious state, we did not consider it in our approach to avoid possible artifacts. In fact, as recently pointed out by Leendertz and coworkers, “Bats have longer life spans than most other mammals of similar size, can fly over great distances, and are able to clear filovirus infections. This means that seropositive specimens may be sampled for a relatively long time in areas geographically distant from where the specimens were exposed to ebolaviruses. Hence, the sampling area might not represent the original infection hot spot.”13. Consequently, we mostly rely on observations reporting the presence of bats colonies and expected presence/absence and predict the infectious state using our modeling SIR framework (Methods).

In terms of the input, we divided the set of observations (presence-absence data) into training and testing sets using different percentages (training/test): 30%/70%, 50%/50% and 70%/30%. Our results indicated that there was not significant difference in the accuracy of the regression using different training/testing ratios. As for the environmental/geographical parameters, we tested the enhanced vegetation index (EVI), precipitation (PRE), daily air temperature (TMP), land cover index (LND), ground elevation (ELV), human population density (POP), latitude (LAT), and longitude (LON)31,32,33,34,35,36,37 (see Methods for dimensional treatment). Thus, in addition to the environmental conditions at specific locations, we used variables that account for information related with the vegetation and human settlement since some evidence suggests that forest loss could drive bats (and other possibly infected hosts) into closer contact with humans.

The tested variables were sorted by increasing value of the correlation with the observations of bats in the sample and so ranked in the following order, EVI, PRE, TEM, LND, ELV, LON, POP and LAT. We repeated the regression analysis 500 times, splitting the random samples of observations into training and testing sets (training/test ratio 50%/50%). For each random partition of the data in training and testing sets, the least-squares method provides a slightly different estimate of the coefficients α’s that weight the relative importance of the variables, (see Methods), which in turn gives slightly different estimates of the carrying capacity K0 at the various locations. Our results indicate that, as expected, the accuracy of all regression models tested increases as the number of environmental variables increases. Yet, regardless of the regression model, the accuracy reaches a plateau when the following five predictors are considered: EVI, PRE, TMP, LND, and ELV, see Fig. 1. The additional variables (POP, LAT and LON) do not increment significantly the accuracy. This suggests that their contribution is embedded within the other variables.

The accuracy in the calibration of K0 increases as cross terms between independent variables measuring non-linear relations among variables are added to the model. Figure 1 shows the adjusted R2 coefficient for the three considered regression models as a function of the data variables by means of a boxplot. The GLC regression model provides a good fitting such that, using just the five predictors mentioned above, the median of the normalized residual standard error (RSE) and adjusted R2 are 27.0% and 0.78 respectively. While the GPC model provides a similar accuracy, the lack of a significant improvement does not justify the use of a more complex model. We notice that our results show that cross terms are required in the regression and, consequently, the carrying capacity is not simply the result of an additive process of environmental factors but a non-linear combination of them.

In summary, as a function of the relevant environmental parameters, using the GLC regression model, the carrying capacity reads:

$$\begin{array}{rcl}{K}_{0}(x,y) & = & 23+736\cdot EV{I}_{n}+283\cdot PR{E}_{n}-95\cdot TM{P}_{n}-281\cdot LN{D}_{n}\\ & & +\,994\cdot EL{V}_{n}-2203\cdot EV{I}_{n}\times PR{E}_{n}-585\cdot EV{I}_{n}\times TM{P}_{n}\\ & & +\,191\cdot EV{I}_{n}\times LN{D}_{n}-252\cdot EV{I}_{n}\times EL{V}_{n}+1640\cdot PR{E}_{n}\times TM{P}_{n}\\ & & +\,-\,185\cdot LN{D}_{n}\times PR{E}_{n}+400\cdot LN{D}_{n}\times TM{P}_{n}-2023\cdot EL{V}_{n}\times TM{P}_{n}\\ & & +\,277\cdot EL{V}_{n}\times LN{D}_{n}-142\cdot EL{V}_{n}\times PR{E}_{n}\end{array}$$
(1)

where the subscript, n, indicates normalized dimensionless indexes and the numerical factors are given in units of bats/km2 (see Methods).

Figure 2 shows the inferred map for K0(x, y) and reveals that the model, Eq. (1), allocates abundant resources in the tropical area of the rainforest and the surrounding savanna, while assigns a scarce value to K0 in the desertic regions of Sahara and Namibia, as expected. The figure also shows that K0 is rich in the east area of Africa along the coast of the Indian ocean from Somalia through Mozambique and in the Madagascar island (see Discussion). Notably, some of the considered species are believed not to be present in those geographical locations but we stress that we aim at capturing a generic, effective, behavior of all bats species able to act as a vector of filoviruses. Importantly, this spatial distribution map of K0 is consistent with the results obtained by other approaches using alternative machine learning techniques38,39. Also, the fact that the regression method predicts correctly the presence of bats at locations where no observations where provided, e.g. Madagascar38,39, shows the robustness of our approach to obtain a general spatial distribution map of the carrying capacity in the context of bat species associated with filoviruses.

Once the carrying capacity for the bats has been calibrated, we use this information in a SIR mathematical model that estimates the density of bats in different infectious states (see Methods).

### Case Study: Liberia, Sierra Leone, Guinea

In order to test the validity of our framework against epidemic data, we focus on the 2014 Ebola outbreak. Thus, we run simulations of the SIR model using for each month of the year average values of the environmental parameters over 15 years from 2000 to 2015, in the region of Africa where the 2014 outbreak developed. Ideally, we would test our results against bat serosurvey datasets (see10) instead of against human cases initiating spillovers. However, the aforementioned lack of precise and recent time stamps of the serosurveys makes it difficult to correlate them with our results. Yet, as shown below, our data agrees with current knowledge about bat infection. Geographically, the first human case was identified in the district of Gueckedou, at the village of Meliandou (Lon: −10.13°, Lat: 8.62°), in Guinea, then the epidemic rapidly spread in Liberia (78 cases) and Sierra Leone (254 cases)40.

We focus our analysis on a region centered at Meliandou that comprises 106 km2 at a resolution (grid) of 10 × 10 km2: the minimum/maximum longitude and latitude range from −17.0° to 7.0° and from 4.0° to 14.0° degrees, respectively. That is, we obtain results for ~104 locations. Our approach additionally provides a monthly temporal resolution for each of those points. To conveniently sample the different parameters of the SIR model (see Methods) we generate 512 realizations using Latin hypercube and compute bounds on the averages by performing a sensitivity analysis on β = {0, 0.5, 1.0}, the parameter that weights the importance of the environmental pressure over the bat migration process.

Figure 3 shows time series of the averages of infected bat density and of the basic reproduction number in two locations within our region of interest: Meliandou and a location far away from there, near Bamako in Mali. We can observe a counterphase seasonal variation. Our results reveal the seasonal appearance of infection peaks depending on the location. Remarkably, a peak of infection at Meliandou is noticeable during the months when the outbreak started. The figure also highlights the importance of coupling the seasonal resources with the infection dynamics: locations with no infection might reveal an outburst associated with changes in the environmental factors (see Movie S1). Our analysis predicts two yearly peaks of infection at Meliandou that coincide with the birthing seasons41, in agreement with studies about other filoviruses (Marburgvirus)42. However, at Bamako our approach only predicts a single peak during the year and suggests the key role played by environmental factors in EBOV propagation. The inferred correlation between the basic reproduction number and the density of infected bats, Fig. 3C, indicates that the infection among bats propagates when densities of infected bats are above $$7.30\frac{{\rm{bats}}}{{{\rm{km}}}^{2}}$$.

Figure 4 shows the average estimate of total and infected bat densities obtained with our framework using averages of the environmental data from 2000 to 2015 for the month of December, when the outbreak started (see also Movies S1S3). On the maps, the location of Meliandou is highlighted with a black circle and the square marks indicate the location of confirmed human cases as collected by WHO40.

Our framework is able to link the niche of EBOV infected bats with the region at risk for human infection. Yet, we notice that in the case of the 2014 epidemic only the initial case has been officially linked to a potential contact with an infected animal and genomic data seem to invalidate the possibility of additional instances of human infection due to the zoonotic niche43,44. Still, our analysis identifies several risk hotspots for bat infection in the region (Movie S1). In the epidemic region, the estimated percentage of infected bats ranges from 4.0% to 6.0% of the bat population and peaks almost at 8.0% in the South of Liberia and West of Ivory Coast, while in the remaining areas the percentage is below 3.0%. It is also worth noting that this area extends beyond the region classified as rainforest according to the Köppen-Geiger climate classification45 where fruit bats mainly assemble and suggests that other bat species play a role in the propagation of the disease.

## Discussion

Here we propose a framework for understanding the spatio-temporal distribution of bats infected by filoviruses that combines data regression, mathematical modeling, and a parameter variability analysis. In this regard, the combination of species distribution modelling with mechanistic epidemiological models is a promising avenue of research for predicting the risk of outbreaks46. Here, by training and comparing different regression models, our approach identifies the main environmental parameters that determine the carrying capacity of bats: vegetation index, elevation, precipitation, land cover and air temperature. The inferred carrying capacity is fed into a compartmental SIR model that accounts for the climate/environmental pressure and, together with a sampling technique to account for the parameters uncertainty, we are able to provide quantitative predictions about the dynamics of bat populations in space and time as well as their infectious state.

Our approach is based on a number of assumptions worth discussing. First, in the case of other filoviruses (e.g. Marburg) evidence strongly suggests that bats are the reservoir. Yet, in the case of EBOV, while many researchers support this hypothesis, this issue is still under debate5,6,10,11,13. In this regard, our study, by predicting a peak of infectious bats where and when the 2014 outbreak started supports the idea that bats are an EBOV reservoir. Yet, we acknowledge that correlation does not imply causation, and consequently, our study must be considered as an additional, but indirect, argument. In fact, filovirus infection is a convoluted problem where other mechanisms play an all-important role: the effects of other infected hosts, the contact with infected primate carcasses (bushmeat) or bat feces, and/or the human-wildlife interaction are key epidemic drivers that we have disregarded herein. Consequently, our analysis cannot be considered as a way to pinpoint the sufficient conditions for an outbreak to develop through any mechanism, but as a tool to assess the risk of having sufficient conditions from the point of view of bats populations driven by environmental factors. As a matter of fact, there is no point in analyzing the months following the epidemic spillover with our framework, because then the infection dynamics rely mostly on human-to-human interactions, rather than on the behavior of the zoonotic niche4. In any case, our approach and results provide important information that can be used by policy-makers, and during decision-making, for preventing and fighting against filovirus outbreaks and in particular Ebola. Thus, at any given location of Africa our framework delivers guidance about the specific periods of the year for which an outbreak could possibly appear due to the bats. For example, Fig. 3 reveals that Meliandou is mostly at risk during March-May and during November-December and, in contrast, Bamako during the period June-September. This dynamics reflects, in agreement with previous results, the importance of birth pulses in the context of filoviruses42. We stress that we do not implement explicitly the existence of birth pulses within our framework; instead we couple the abundance of local resources with the growth rate of the population in our mathematical model and the birth pulses develop as an outcome of enviroclimatic factors. Also, our results help to identify geographical hotspots where surveillance can be more effective (Movie S1). In this regard, recent results have analyzed the importance of forest fragmentation in EBOV outbreaks47. The predictions of our framework are in agreement with those results (cf. Figs 1b and 4 in47) and highlight the importance of linking environmental pressure and bats mobility28. Second, we have calibrated the carrying capacity of our model using only some species of bats. Despite this, the results of our approach are representative also of other bat species that have been found positive for EBOV11. In fact, there is a large overlap between the regions where these insectivorous bats are endemic and the areas populated by the fruit bats investigated in our work. As a consequence, we have included environmental conditions that favor also the presence of other species. As a proof of this, our prediction for the carrying capacity (Fig. 2B) indicates presence at locations (e.g. Madagascar) where the fruit bats considered in our study are not present, but insectivorous bats are. In other words, by including in our calibration data regions where multiple bat species coexist we are not restricting our analysis to the species mentioned in the manuscript for calibration. We are aware (see Methods) that the species that are a competent reservoir in the context of filoviruses (in particular EBOV) are still under debate. However, in the context of filoviruses prevention it is better to incorporate a false positive than a false negative. For this reason, we decided to include in the analysis also some species for which the debate is still open. Third, with regard to the mathematical SIR model, we have assumed that the transmission mechanism is density- instead of frequency-dependent. Our assumption is based on the same arguments used by other researchers modeling the filovirus infection dynamics in bats24: demographic studies have shown that bats usually roost at high densities that are rather independent of the increase of the colony size48 and the transmission of filoviruses among them seems to rely on contact with fluids (feces and urine)10. Indeed EBOVs have been isolated from bat feces 21 days after infection49. These facts point towards a density-dependent transmission modeling approach50.

As for the applicability of our methodology to other locations, our framework was calibrated for the African continent, but adapting it to other geographic areas is straightforward, since the environmental parameters are easily accessible for most parts of the world at high resolution and for several time windows. In that regard, we stress that environmental drivers are crucial, yet insufficient, for establishing the zoonotic niche and data analysis must be complemented by modeling efforts to adequately pinpoint regions at geographical risk of infection. Also, the lack of knowledge of some parameter values as well as their variability requires probabilistic sampling, to address their uncertainty.

Our study also suggests, and the 2017 outbreak confirms, that the diseases with a filovirus origin are latent in the African continent and further outbreaks are likely to appear in the future, unless a widespread vaccination campaign is implemented, when it is available. Also, according to our study, the appearance of outbreaks is tightly linked to fluctuations in the environmental conditions that determine strongly both the migration patterns of bats and their infection dynamics. Among the environmental drivers, both elevation and land cover index are slow-changing variables compared to the vegetation index, the air temperature or the rainfall. Consequently, the sooner and the more comprehensive the information about these factors is available, the better the predictive character of our framework for identifying the risk of outbreaks will be. Recent data initiatives, as Google Earth Engine, certainly contribute positively towards that end. The predictive character of our approach also relies on the quality and the quantity of information about the presence of bats at specific locations and on the quantification of several parameters related to the bats physiology and their interactions with the environment, including birth and death rates, the rate of transmission/recovery of/from the disease, and the rate of depletion of natural resources. In that regard, the broader use of remote telemetry and the systematic implementation of quantitative approaches in ecology will help to cover data gaps.

Another point worth discussing is the role played by bat mobility to spread filoviruses. Here we do not aim at determining the strain propagation mechanisms, i.e. a strain could slowly spread from colony to colony from Central Africa or, alternatively, background infection levels have always been prevalent. Still, we envision a reinforcement mechanism where mobility plays a key role. As shown in Fig. 3, basal levels of infection do not imply disease spreading; yet, population levels can locally increase due to migration and seasonality28 thus triggering an outbreak according to our study.

A final comment is about possible strategies to overcome additional sources of uncertainty to accurately set confidence bounds for our predictions. Here we have calibrated the carrying capacity of the zoonotic niche training different regression models by using environmental data for a ten year period. While the accuracy of the regression is good, there is certainly a source of variability in the carrying capacity that we have disregarded. Including that randomness depends on the characterization of this “random field” and a rigorous sampling of its realizations to represent accurately its statistical properties. Given the spatial and temporal resolution considered in our framework, that task is computationally unrealistic, even on supercomputing facilities, when using traditional methods, e.g. Monte Carlo. This poses the interesting question of developing computationally efficient and mathematically rigorous methods to account for this unavoidable source of uncertainty in the context of ecology. Work in this direction is in progress.

## Methods

### Carrying Capacity Calibration and Data Sets

Herein, we aim at obtaining a distribution map of the “bare” carrying capacity as a function of environmental variables that accounts for the available resources for bats species associated with filoviruses in Africa. To that end, in this study we analyze the geographical distribution of four species of bats from sub-Saharian Africa: Eidolon helvum, Hipposideros gigas, Hypsignathus monstrosus, and Rousettus aegyptiacus30. These species were selected because a) there is evidence that suggests their relation with filoviruses infection cases, b) they cluster in large colonies and contribute importantly to the chiroptera biomass, and c) quantitative data is available on their presence at specific locations and colony sizes, which we can use in our regression approach. Some researchers have claimed that Rousettus aegypticus does not appear to be a competent reservoir in particular for EBOV51. However, this claim is contradicted by the findings of Pourrut et al. during the Gabon outbreak, where they tested field bats for Marburg and Ebola52.

By mixing species with a variety of life characteristics (i.e. insectivorous and frugivorous bats, tree dwelling and cave roosting bats), we expect to capture effectively a collective, non-specific, trait about the carrying capacity of bats. This overall behavior about bat species is further explored by sampling the parameter space of our model as explained below. We acknowledge that by discarding other bat species that have been shown to contribute to the filovirus zoonotic niche, e.g. Epomops franqueti and Myonycteris torquata10, our results can be thought to be biased. However, we stress that the geographical distribution of these species overlaps with that of the species considered in our study and, as shown in the Results section, our results are in good agreement with current knowledge about the zoonotic niche and future field studies would allow to refine our findings easily by implementing the methodology introduced here.

The maximum size of a colony for each of the considered species is approximately 500,000 (Eidolon helvum), 1,000 (Hipposideros gigas), 100 (Hypsignathus monstrosus), and 5,000 (Rousettus aegyptiacus) individuals30. Although it is difficult to accurately estimate spatial densities of bats colonies53, studies conducted on Eidolon helvum, the most common fruit bat in Africa, show that colonies forage in regions near the roost within a radius of action that ranges between 10 km and 20 km14,54. Here we assume that a radius of twice the average size of the foraging distance, 30 km, provides a realistic upper bound of the colony area of influence: 2,800 km214,15,30. Accordingly, the density of a Eidolon helvum colony for example, can be estimated equal to $$180\frac{{\rm{bats}}}{{{\rm{km}}}^{2}}$$. We take these densities as indirect measurements of the “bare” carrying capacity, K0, at a specific location to train different regression models (see below); that is, the maximum density of bats that can be sustained by the available resources in a particular region.

We hypothesize that it is possible to correlate K0 with a set of environmental parameters that are important for the habitat of fruit bats, such as vegetation, precipitation, temperature and ground elevation. To this end, we implement a regression approach testing three models: a multi-linear model (MLN), a generalized multi-linear model with linear cross terms (GLC), and a second order polynomial model with linear cross terms (GPC).

Input data in the regression models are values of environmental parameters averaged over ten years (period from 2000 to 2010) retrieved from the Google Earth Engine database collection at a resolution (grid) 10 × 10 km2. Environmental/climate data was obtained from the databases of the NASA Land Processes Distributed Active Archive Center (LP DAAC), USGS/Earth Resources Observation and Science (EROS) Center, Sioux Falls, South Dakota31,32,33,34,35,36,37. All map images shown in this study were generated using the R software packages “maps”, “gdal” and “raster”55,56,57.

We tested eight environmental parameters: enhanced vegetation index (EVI, dimensionless), precipitation $$({\rm{PRE}},{\rm{units}}:\frac{{\rm{mm}}}{{\rm{5}}\cdot {\rm{days}}})$$, daily air temperature (TMP, units: °C), land cover index (LND, dimensionless), ground elevation (ELV, units: m), population density (POP, units: individuals/km2), latitude (LAT, units: °), and longitude (LON, units: °)31,32,33,34,35,36,37.

The EVI accounts for the amount of vegetation at a specific location (0 if low and 1 if high) and optimizes the vegetation signal with improved sensitivity in high biomass regions32. Fruit bats are present in geographic regions with medium/high vegetation level. The land cover index, LND, follows the MODIS classification scheme and associates an index to different geographic areas: there are 7 major categories and 17 subcategories listed in Table 131,37. We convert all satellite data entries to normalized dimensionless indexes by using the maximum value found for each variable in the analyzed data (see Results): PRE MAX  = $$100\frac{{\rm{mm}}}{5\cdot {\rm{days}}}$$, LND MAX  = 15, ELV MAX  = 5, 000 m, TMP MAX  = 50 °C, POP = $$400\frac{{\rm{individuals}}}{{{\rm{km}}}^{2}}$$, LON MAX  = 50°, and LAT MAX  = 40°. We point out that LND is a categorical, rather than a quantitative variable, but the subcategory indexes are sorted by density of vegetation. Thus, for the sake of consistency with the rest of the predictors, we decided to treat the dummy variable associated with LND using rational numbers in the range [0, 1] rather than integers.

An additional input to the regression models is the estimated bare carrying capacity, K0, at locations where field studies have revealed the presence of bat colonies. We use data as collected by Bergmans29. We reiterate that although there is some data available about bats obtained from serological surveillance studies indicating the infectious state, we did not consider it in our approach to avoid possible artifacts. Consequently, we rely on observations reporting the presence of bats colonies and expected presence/absence and predict the infectious state using our modeling SIR framework.

We complement this information with (a) expected presence data by imposing the presence of bat colonies at locations where the IUCN database (a geographical range database)30 suggests the existence of the considered species and, (b), with absence data, i.e. K0 = 0, at locations where environmental conditions challenge bat survival (e.g., Sahara dessert), see Fig. 2. Still, since we aim at correlating as precisely as possible environmental factors and the carrying capacity, we keep the number of these additional instances, where presence/absence has not been certified, to a minimum that yields an acceptable accuracy of the regression analysis.

The regression models used in our approach satisfy the following functional forms:

$${K}_{0}(x,y)={\alpha }_{0}+\sum _{i=1}^{p}{\alpha }_{i}\cdot {v}_{i}(x,y)({\rm{MLN}})$$
(2)
$${K}_{0}(x,y)={\alpha }_{0}+\sum _{i=1}^{p}{\alpha }_{i}\cdot {v}_{i}(x,y)+\sum _{i=1}^{p}\sum _{j=i+1}^{p}{\alpha }_{ij}\cdot {v}_{i}(x,y)\cdot {v}_{j}(x,y)({\rm{GLC}})$$
(3)
$${K}_{0}(x,y)={\alpha }_{0}+\sum _{i=1}^{p}{\alpha }_{i}\cdot {v}_{i}(x,y)+\sum _{i=1}^{p}\sum _{j=i}^{p}{\alpha }_{ij}\cdot {v}_{i}(x,y)\cdot {v}_{j}(x,y)({\rm{GPC}})$$
(4)

where p represents the number of environmental variables, the terms α0 (basal value of the carrying capacity), α i , and α ij are the regression coefficients $$({\rm{units}}:\frac{{\rm{bats}}}{{{\rm{km}}}^{2}})$$, and v i and v j are the dimensionless environmental variables that are functions of the longitude and the latitude (x and y respectively). The coefficients of the regression model are obtained by applying the least-squares method (see Results).

### Compartmental SIR Model for Filovirus Infection and Parameter Sampling

Agent-based epidemiology modeling allows a high level of detail. As a consequence, more parameters need to be fitted/estimated and these models are computationally expensive. In case multiple scenarios need to be explored for evaluating uncertainties, as we do here, this modeling scheme is therefore not suitable. In contrast, compartmental coarse-grained descriptions require fewer parameters to fit/calibrate and are computationally efficient. Thus, we couple regression data, i.e. the inferred carrying capacity K0, with a compartmental model that we recently proposed for the dynamics of bat populations in the context of filoviruses infection: we refer the reader to28 for the rationale underlying the different processes considered in our modeling, including the inclusion of the recovery-from-infection state. The model accounts for densities of fruit bats that are either Susceptible, B S , Infected, B I , or Recovered, B R , from EBOV infection:

$$\frac{\partial {B}_{S}}{\partial t}={b}_{{K}^{\ast }}B-c{B}_{S}-|{a}_{{K}^{\ast }}|\frac{{B}_{S}B}{K}-e{B}_{S}{B}_{I}+f\,{B}_{R}+{D}_{{K}^{\ast }}{\nabla }^{2}{B}_{S}$$
(5)
$$\frac{\partial {B}_{I}}{\partial t}=-\,c{B}_{I}-|{a}_{{K}^{\ast }}|\frac{{B}_{I}B}{K}+e{B}_{S}{B}_{I}-d{B}_{I}+{D}_{{K}^{\ast }}{\nabla }^{2}{B}_{I}$$
(6)
$$\frac{\partial {B}_{R}}{\partial t}=-\,c{B}_{R}-|{a}_{{K}^{\ast }}|\frac{{B}_{R}B}{K}+d{B}_{I}-f\,{B}_{R}+{D}_{{K}^{\ast }}{\nabla }^{2}{B}_{R}$$
(7)
$$\frac{\partial K}{\partial t}=-\,\gamma B+r({K}_{0}-K)$$
(8)

Eqs (57) describe the dynamic of bats in space and time as a function of the resources (carrying capacity) K, where B = B S  + B I  + B R stands for the total density of bats at a given location.

The parameters of the model are the birth rate $${b}_{{K}^{\ast }}$$, the death rate c, the virus transmission rate e, the recovery from infection rate d, and the rate at which bats return to a susceptible state, f. In addition, the coefficient $${a}_{{K}^{\ast }}={b}_{{K}^{\ast }}-c$$ corresponds to the population growth rate, and $${\nabla }^{2}=\frac{{\partial }^{2}}{\partial {x}^{2}}+\frac{{\partial }^{2}}{\partial {x}^{2}}$$ to the Laplacian operator that accounts for the spatial mobility of bats; and $${D}_{{K}^{\ast }}$$ is the diffusion coefficient. Notice that in the model, based on the available data about filovirus infection in bats, we consider that the vectors are born virus free42. It is worth noting that our model assumes a density-dependent transmission mechanism. This is the same approach used in other models based on the bat roosting, demography, and the means of filovirus transmission24 (see Discussion).

Eq. (8) describes the dynamics of resources as a function of the bare carrying capacity K0 estimated by the regression approach, where r represents the natural depletion rate of resources and γ is the consumption rate of resources by bats. The environmental pressure is implemented in the model by modulating both the birth, $${b}_{{K}^{\ast }}$$, and the diffusion rate, $${D}_{{K}^{\ast }}$$, as a function of K*. This parameter describes a threshold value of the carrying capacity for which there are changes in $${b}_{{K}^{\ast }}$$ and $${D}_{{K}^{\ast }}$$. On one hand, if K is smaller than K*, then $${b}_{{K}^{\ast }}=0$$ and $${D}_{{K}^{\ast }} > 0$$. That is, if the environmental factors challenge bat survival, then bats migrate. On the other hand, if K is greater than K*, then $${b}_{{K}^{\ast }} > 0$$ and $${D}_{{K}^{\ast }}=0$$. If the conditions in terms of the available resources are satisfactory for the sustainability of the population, then bats do not migrate (see28 for details). We point out that this birth and mobility dynamics recapitulates effectively two important mechanisms for pathogen spreading: birth pulsating dynamics and habitat fragmentation (see Discussion). As a function of the parameters of the model and the bare carrying capacity, K0, the basic reproduction number, $${ {\mathcal R} }_{0}$$, can be estimated28:

$${ {\mathcal R} }_{0}=\frac{e}{{e}_{c}}-\,\frac{|{b}_{{K}^{\ast }}-c|}{{K}^{\ast }{e}_{c}}$$
(9)

We recall that $${ {\mathcal R} }_{0}$$ indicates how the disease propagates among bats, such that if $${ {\mathcal R} }_{0} > 1$$ the infection spreads in the population and if $${ {\mathcal R} }_{0} < 1$$ the infection dies out58.

The system of Eqs (58) has three different stationary states, $$\{{K}^{st},{B}_{S}^{st},{B}_{I}^{st},{B}_{R}^{st}\}$$. On one hand, {K0, 0, 0, 0} corresponds to the trivial null state: no bats, and the resources are at their bare capacity. This state is stable if $${a}_{{K}^{\ast }} < 0$$. The second steady state condition corresponds to the infection-free equilibrium {μK0, μK0, 0, 0}, where $$\mu =\frac{r}{r+\gamma }$$. This state is stable if $${a}_{{K}^{\ast }} > 0$$ and the infection coefficient e is smaller than a critical value $${e}_{c}=\frac{(c+d)}{\mu (1-\beta ){K}_{0}}$$, where the coefficient β [0, 1] tunes the value of K* in the interval [μK0, K0]: K* = μK0(1−β) + K0β. For example, when β = 0.5, then $${K}^{\ast }=\frac{(1+\mu ){K}_{0}}{2}$$. The third steady state corresponds to the infection stage, {μK0, α S K0, α I K0, α R K0}, that is stable if $${a}_{{K}^{\ast }} > 0$$ and e > e c , where:

$${\alpha }_{S}=\mu (1-\beta )\frac{{e}_{c}}{e}$$
(10)
$${\alpha }_{I}=\frac{\mu (1-\beta )(c+f)}{(c+d+f)}(1-\frac{{e}_{c}}{e})$$
(11)
$${\alpha }_{R}=\frac{\mu (1-\beta )d}{(c+d+f)}(1-\frac{{e}_{c}}{e})$$
(12)

We solve numerically the SIR model by means of an explicit Forward-Time Central-Space (FTCS) finite differences method in a rectangular lattice59. Each tile of the lattice represents 100 km2, i.e. a spatial resolution of 10 km that is the same of the geographic maps used for the regression analysis. In order to avoid transient effects in our simulations, the initial values for B S , B I , and B R correspond to the stationary densities as prescribed by Eqs (1012). The integration time step in our simulations is $$\frac{1}{100}$$ days. This guarantees the stability of the integration method and ensures that we capture the smallest time scale relevant in the dynamics of the bats population. Finally, the equations are integrated over one month period to ensure that stationary conditions are reached.

The lack of sufficient data about several parameters involved in the compartmental model makes it difficult to obtain an accurate calibration and hence it diminishes the predictive capabilities of our framework. For example, available data about the transmission rate e among bats for EBOV is very limited, therefore we assumed that this rate is comparable to the infection rate of bats infected with Marburg. We estimated e observing seasonal variation of the bat seropositivity as given in42. Here we address the uncertainty about the characteristic rates by means of a sampling approach of the parameter space. Thus, we generate random sets of 512 instances for each parameter, assuming that each of them satisfies a uniform distribution within bounds as suggested by data and/or physical constraints. The list of sampled parameters and their estimated minimum and maximum values are given in Table 2. The sampling method we utilize is Latin hypercube sampling (LHS)60. We average the results of our simulations using the 512 sampling instances (i.e. sets). Assuming normality for the distribution of the mean value of the bat population, this sample size provides a ±2.0% accuracy on such mean with 95% confidence level. These confidence bounds were assessed based on the results of the proposed regression model61. The general flowchart of our framework is schematically presented in Fig. 5.

## References

1. 1.

Bausch, D. G. & Schwarz, L. Outbreak of ebola virus disease in Guinea: where ecology meets economy. PLoS neglected tropical diseases 8, e3056 (2014).

2. 2.

World Health Organization (WHO). Building the legacy of Ebola: Survivors, health systems, and a blueprint for research and development. Tech. Rep. January 2017 (2017).

3. 3.

Baize, S. et al. Emergence of Zaire Ebola virus disease in Guinea. The New England journal of medicine 371, 1418–1425 (2014).

4. 4.

Chowell, G. & Nishiura, H. Transmission dynamics and control of Ebola virus disease (EVD): a review. BMC medicine 12, 196 (2014).

5. 5.

Spengler, J. R., Ervin, E. D., Towner, J. S., Rollin, P. E. & Nichol, S. T. Perspectives on West Africa Ebola Virus Disease Outbreak, 2013–2016. Emerging infectious diseases 22, 956–963 (2016).

6. 6.

Leroy, E. M. et al. Fruit bats as reservoirs of Ebola virus. Nature 438, 575–576 (2005).

7. 7.

Hayman, D. T. S. et al. Long-Term Survival of an Urban Fruit Bat Seropositive for Ebola and Lagos Bat Viruses. Plos One 5, e11978 (2010).

8. 8.

Hayman, D. T. S. et al. Ebola virus antibodies in fruit bats, Ghana, West Africa. Emerging infectious diseases 18, 1207–1209 (2012).

9. 9.

Leroy, E. M. et al. Human Ebola outbreak resulting from direct exposure to fruit bats in Luebo, Democratic Republic of Congo, 2007. Vector borne and zoonotic diseases (Larchmont, N.Y.) 9, 723–728 (2009).

10. 10.

Olival, K. J. & Hayman, D. T. S. Filoviruses in bats: current knowledge and future directions. Viruses 6, 1759–1788 (2014).

11. 11.

Leendertz, S. A. J., Gogarten, J. F., Düx, A., Calvignac-Spencer, S. & Leendertz, F. H. Assessing the Evidence Supporting Fruit Bats as the Primary Reservoirs for Ebola Viruses. Eco Health 13, 18–25 (2016).

12. 12.

Olivero, J. et al. Mammalian biogeography and the Ebola virus in Africa. Mammal Review 46 (2016).

13. 13.

Leendertz, S. A. J. Testing new hypotheses regarding ebolavirus reservoirs (2016).

14. 14.

Richter, H. V. & Cumming, G. S. Food availability and annual migration of the straw-colored fruit bat (Eidolon helvum). Journal of Zoology 268, 35–44 (2006).

15. 15.

Fahr, J. et al. Pronounced Seasonal Changes in the Movement Ecology of a Highly Gregarious Central-Place Forager, the African Straw-Coloured Fruit Bat (Eidolon helvum). Plos One 10, 1–19 (2015).

16. 16.

House, T. & Jha, P. Epidemiological dynamics of Ebola outbreaks. eLife 3, e03908 (2014).

17. 17.

Gelfand, A. G V: Modeling Ebola. Biomedical Computation Review E53–64 (2016).

18. 18.

Shen, M., Xiao, Y. & Rong, L. Modeling the effect of comprehensive interventions on Ebola virus transmission. Scientific Reports 5, 15818 (2015).

19. 19.

Chretien, J.-P., Riley, S. & George, D. B. Mathematical modeling of the West Africa Ebola epidemic. eLife 4, e09186 (2015).

20. 20.

Drake, J. M. et al. Transmission Models of Historical Ebola Outbreaks. Emerging infectious diseases 21, 1447–1450 (2015).

21. 21.

Valeri, L. et al. Predicting subnational ebola virus disease epidemic dynamics from sociodemographic indicators. Plos One 11, 1–16, https://doi.org/10.1371/journal.pone.0163544 (2016).

22. 22.

Xia, Z.-Q. et al. Modeling the transmission dynamics of Ebola virus disease in Liberia. Scientific reports 5, 13857 (2015).

23. 23.

Lewnard, J. A. et al. Dynamics and control of Ebola virus transmission in Montserrado, Liberia: a mathematical modelling analysis. The Lancet. Infectious diseases 14, 1189–1195 (2014).

24. 24.

Hayman, D. T. S. Biannual birth pulses allow filoviruses to persist in bat populations. Proceedings. Biological sciences/The Royal Society 282, 20142591 (2015).

25. 25.

Guo, Z. et al. Predicting and evaluating the epidemic trend of ebola virus disease in the 2014-2015 outbreak and the effects of intervention measures. Plos One 11, 1–17, https://doi.org/10.1371/journal.pone.0152438 (2016).

26. 26.

Judson, S. D., Fischer, R., Judson, A. & Munster, V. J. Ecological contexts of index cases and spillover events of different ebolaviruses. PLOS Pathogens 12, 1–17, https://doi.org/10.1371/journal.ppat.1005780 (2016).

27. 27.

Nyakarahuka, L. et al. Ecological Niche Modeling for Filoviruses: A Risk Map for Ebola and Marburg Virus Disease Outbreaks in Uganda. PLoS Currents 9 (2017).

28. 28.

Buceta, J. & Johnson, K. Modeling the Ebola Zoonotic Dynamics: Interplay between Enviroclimatic Factors and Bat Ecology. Plos One 12(6), e0179559 (2017).

29. 29.

Bergmans, W. T and Biogeography of African Fruit Bats. Beaufortia 39 (1989).

30. 30.

IUCN. The IUCN Red List of Threatened Species. http://www.iucnredlist.org (2016).

31. 31.

Google Earth Engine Team. Google earth engine: A planetary-scale geo-spatial analysis platform. https://earthengine.google.com (2015).

32. 32.

Huete, A. et al. Overview of the radiometric and biophysical performance of the MODIS vegetation indices. Remote Sensing of Environment 83, 195–213 (2002).

33. 33.

Danielson, J. J. and Gesch, D. B. Global Multi-resolution Terrain Elevation Data 2010. Tech. Rep. Report 2011–1073 (2011).

34. 34.

Funk, C. et al. A quasi-global precipitation time series for drought monitoring: U.S. Geological Survey Data Series 832, https://doi.org/10.3133/ds832 (2014).

35. 35.

NASA LP DAAC. NASA EOSDIS Land Processes DAAC, USGS Earth Resources Observation and Science (EROS) Center, Sioux Falls, South Dakota. https://lpdaac.usgs.gov (2015).

36. 36.

Linard, C., Gilbert, M., Snow, R. W., Noor, A. M. & Tatem, A. J. Population Distribution, Settlement Patterns and Accessibility across Africa in 2010. Plos One 7, e31743 (2012).

37. 37.

Friedl, M. A. et al. Remote Sensing of Environment MODIS Collection 5 global land cover: Algorithm refinements and characterization of new datasets. Remote Sensing of Environment 114, 168–182 (2010).

38. 38.

Pigott, D. M. et al. Mapping the zoonotic niche of Ebola virus disease in Africa. Elife 3, 1–29 (2014).

39. 39.

Peterson, A. T., Bauer, J. T. & Mills, J. N. Ecologic and geographic distribution of filovirus disease. Emerging infectious diseases 10, 40 (2004).

40. 40.

WHO. Ebola Data and Statistics. http://apps.who.int/gho/data/node.ebola-sitrep.ebola-country?lang=en (2015).

41. 41.

Thomas, D. & Marshall, A. Reproduction and growth in three species of West African fruit bats. Journal of Zoology 202, 265–281 (1984).

42. 42.

Amman, B. R. et al. Seasonal pulses of Marburg virus circulation in juvenile Rousettus aegyptiacus bats coincide with periods of increased risk of human infection. PLoS pathogens 8, e1002877 (2012).

43. 43.

Gire, S. K. et al. Genomic surveillance elucidates Ebola virus origin and transmission during the 2014 outbreak. Science (New York, N.Y.) 345, 1369–1372 (2014).

44. 44.

Dudas, G. et al. Virus genomes reveal factors that spread and sustained the Ebola epidemic. Nature 544, 309–315 (2017).

45. 45.

Kottek, M., Grieser, J., Beck, C., Rudolf, B. & Rubel, F. World Map of the Köppen-Geiger climate classification updated. Meteorologische Zeitschrift 15, 259–263 (2006).

46. 46.

Redding, D., Moses, L., Cunningham, A., Wood, J. & Jones, K. Environmental-mechanistic modelling of the impact of global change on human zoonotic disease emergence: a case study of Lassa fever. Methods in Ecology and Evolution 7, 646–655 (2016).

47. 47.

Rulli, M. C., Santini, M., Hayman, D. T. S. & D’Odorico, P. The nexus between forest fragmentation in Africa and Ebola virus disease outbreaks. Scientific Reports 7, 41613 (2017).

48. 48.

Hayman, D. T. S. et al. Demography of straw-colored fruit bats in Ghana. Journal of mammalogy 93, 1393–1404 (2012).

49. 49.

Swanepoel, R. et al. Experimental inoculation of plants and animals with Ebola virus. Emerging infectious diseases 2, 321–325 (1996).

50. 50.

Begon, M. et al. A clarification of transmission terms in host-microparasite models: numbers, densities and areas. Epidemiology and Infection 129, 147–153 (2002).

51. 51.

Jones, M. E. et al. Experimental inoculation of Egyptian rousette bats (Rousettus aegyptiacus) with viruses of the Ebolavirus and Marburgvirus genera. Viruses 7, 3420–3442 (2015).

52. 52.

Pourrut, X. et al. Large serological survey showing cocirculation of Ebola and Marburg viruses in Gabonese bat populations, and a high seroprevalence of both viruses in Rousettus aegyptiacus. BMC infectious diseases 9, 159 (2009).

53. 53.

Hayman, D. T. S. et al. Demography of straw-colored fruit bats in Ghana Demography of straw-colored fruit bats in Ghana. Journal of Mammalogy 93, 1393–1404 (2012).

54. 54.

Richter, H. V. & Cumming, G. S. First application of satellite telemetry to track african straw-coloured fruit bat migration. Journal of Zoology 275, 172–176 (2008).

55. 55.

R Core Team. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria http://www.R-project.org/ (2013).

56. 56.

Bivand, R., Keitt, T. & Rowlingson, B. rgdal: Bindings for the Geospatial Data Abstraction Library https://CRAN.R-project.org/package=rgdal. R package version1.1-10 (2016).

57. 57.

Hijmans, R. J. raster: Geographic Data Analysis and Modeling https://CRAN.R-project.org/package=raster. R package version 2.5-8 (2016).

58. 58.

Keeling, M. J. & Rohani, P. Modeling Infectious Diseases in Humans and Animals (Princeton University Press 2011).

59. 59.

Press, W. H. Numerical Recipes 3rd Edition. The Art of Scientific Computing (Cambridge University Press 2007).

60. 60.

McKay, M. D., Beckman, R. J. & Conover, W. J. A Comparison of Three Methods for Selecting Values of Input Variables in the Analysis of Output from a Computer Code. Technometrics 21, 239–245 (1979).

61. 61.

Lapin, L. Probability and statistics for modern engineering (Waveland Press, Inc. 1998).

62. 62.

Wilkinson, G. S. & South, J. M. Life history, ecology and longevity in bats. Aging cell 1, 124–31 http://www.ncbi.nlm.nih.gov/pubmed/12882342 (2002).

## Acknowledgements

The financial support of Lehigh University thorough a CORE grant is greatly acknowledged. J.B. and P.B. acknowledge financial support from the National Institutes of Health through grant 1R15GM123422-01A1. The authors thank Dr. David Hayman for his fruitful comments. The environmental/climate data was retrieved using the Google Earth Engine tool. Data courtesy of the NASA Land Processes Distributed Active Archive Center (LP DAAC), USGS/Earth Resources Observation and Science (EROS) Center, Sioux Falls, South Dakota, https://lpdaac.usgs.gov.

## Author information

Authors

### Contributions

J.B. and P.B. conceived the research. G.F. performed the simulations and analyses. All authors wrote and reviewed the manuscript.

### Corresponding authors

Correspondence to Paolo Bocchini or Javier Buceta.

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Fiorillo, G., Bocchini, P. & Buceta, J. A Predictive Spatial Distribution Framework for Filovirus-Infected Bats. Sci Rep 8, 7970 (2018). https://doi.org/10.1038/s41598-018-26074-4

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