Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) composition in skeletal muscle have been linked to insulin sensitivity. We evaluated the relationships between skeletal muscle PC:PE, physical exercise and insulin sensitivity. We performed lipidomics and measured PC and PE in m. vastus lateralis biopsies obtained from 13 normoglycemic normal weight men and 13 dysglycemic overweight men at rest, immediately after 45 min of cycling at 70% maximum oxygen uptake, and 2 h post-exercise, before as well as after 12 weeks of combined endurance- and strength-exercise intervention. Insulin sensitivity was monitored by euglycemic-hyperinsulinemic clamp. RNA-sequencing was performed on biopsies, and mitochondria and lipid droplets were quantified on electron microscopic images. Exercise intervention for 12 w enhanced insulin sensitivity by 33%, skeletal muscle levels of PC by 21%, PE by 42%, and reduced PC:PE by 16%. One bicycle session reduced PC:PE by 5%. PC:PE correlated negatively with insulin sensitivity (β = −1.6, P < 0.001), percent area of mitochondria (ρ = −0.52, P = 0.035), and lipid droplet area (ρ = 0.55, P = 0.017) on EM pictures, and negatively with oxidative phosphorylation and mTOR based on RNA-sequencing. In conclusion, PC and PE contents of skeletal muscle respond to exercise, and PC:PE is inversely related to insulin sensitivity.
Type 2 diabetes mellitus (T2DM) affects ~415 million adults worldwide, and is predicted to rise to ~642 million by 2040 (http://www.diabetesatlas.org). Approximately 12% of global health expenditures are spent on diabetes (http://www.diabetesatlas.org). T2DM is characterized by hyperglycemia and strongly associated with insulin resistance, adiposity, and physical inactivity1,2,3.
Physical activity is known to improve insulin sensitivity and glucose tolerance4,5, which might prevent development of T2DM. A single bout of exercise is sufficient to increase skeletal muscle glucose uptake for several hours6. Twelve weeks of exercise can significantly improve whole-body glucose metabolism and insulin sensitivity7. Extensive improvement in insulin sensitivity may occur by combining strength- and endurance training8,9. Many pathophysiological variables influence insulin sensitivity such as muscle mass, maximum oxygen uptake (VO2max), many signal molecules, and tissue fat content7.
Skeletal muscle phosphoacylglycerol composition and different classes of phospholipids are related to membrane fluidity, lipid rafts10, membrane-protein dynamics and insulin receptor kinetics11,12,13. Phosphoacylglycerols are also important for structural integrity of mitochondria, membrane potential, substrate transport, Ca2+ homeostasis, morphological changes in organelles14, and insulin sensitivity15,16,17.
Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) are the major phospholipids in cellular membranes. PC accounts for ∼50%, whereas PE represents 20–30% of the total phospholipid pool18. Studies on skeletal muscle-specific knock-out models of PC- and PE-related enzymes19,20,21 have shown reduced PE synthesis and increased PC:PE ratio, in addition to reduced skeletal muscle mass, endoplasmic reticulum/sarcoplasmic reticulum (ER/SR) Ca2+ ATPase (SERCA) activity, and low exercise performance19,20,21. Thus, there might be a role for the PC:PE ratio in skeletal muscle growth, contraction, exercise performance, and glucose metabolism. Studies on humans with obesity or T2DM, as well as endurance-trained athletes, suggest a role for the PC:PE ratio in skeletal muscle metabolism and insulin sensitivity22.
The aim of our study was to evaluate the skeletal muscle PC:PE ratio in response to long-term physical exercise, as well as potential links between changes in the PC:PE ratio and improved insulin sensitivity. We conducted an intervention with an acute bicycle test before as well as after 12 w of combined strength- and endurance training7 to assess changes in insulin sensitivity and the skeletal muscle PC:PE ratio in normal weight control men and dysglycemic overweight men.
Twenty-six sedentary men were recruited in (1) a normoglycemic, normal weight group (n = 13), and (2) a dysglycemic overweight group (n = 13). In some previous articles from this study, we excluded two men in each group because they differed slightly from the predefined criteria for BMI and glycemia; group 1: F-glucose <5.6 mmol/L and 2 h glucose <7.8 mmol/L and BMI 19–25; group 2: F-glucose ≥5.6 mmol/L and/or 2 h glucose ≥7.8 mmol/L and BMI 27–327. Hence, two of 13 subjects in group 1 had BMI slightly above 25, but were insulin sensitive documented by the clamp. Two of 13 subjects in group 2 had glucose levels just below the cut-offs, but were insulin resistant documented by the clamp. In the present study we compared the results excluding (11 vs. 11) or including (13 vs. 13) these subjects, with no differences in conclusions. Thus, we present the results including all subjects (n = 26).
Diet and exercise
Participants registered their habitual diet in an extensively validated food frequency questionnaire before and after intervention24,25,26,27. Food composition and energy intake were similar during the intervention based on calculated intake using the food database AE-10 and KBS food and nutrients calculation system (KBS Version 7.1, 2013). Alcohol intake was not allowed to exceed two units per day. During testing at baseline and after 12 w the participants consumed a standardized meal after an overnight fast. All men were sedentary before inclusion to the study (<1 exercise session/w for the previous year). A carbohydrate-rich meal including ~2 slices of bread (“Ingers Superrug” from “Bakers,” the amount was adjusted depending on individual energy requirement), apple juice, cheese (total of 30 g fat), and jam, providing 23% of estimated total daily energy expenditure and ingested 90–120 min prior to the bicycle tests and sampling of biopsies. The bicycle tests were typically performed in the morning; the standardized meal was the only intake after overnight fast. Water could be consumed freely.
Acute bicycle tests
An acute bicycle test was performed before as well as after the 12 w exercise intervention. After 10 min warm-up, the participants cycled 45 min at an individual workload equivalent to 70% of their personal VO2max. For the post-test a new workload was calculated corresponding to the post-test VO2max. Participants refrained from strenuous physical activity two days before the test, and had the last standard endurance session three days before post-tests.
Strength and endurance training
The participants underwent combined resistance and endurance exercise for 12 w, including two endurance bicycle sessions (60 min each) and two whole-body resistance-training sessions (60 min each) per week. All exercise sessions were supervised by trained personnel.
Resistance-training included leg press, leg curl, chest press, cable pull-down, shoulder press, seated rowing, abdominal crunches, and back extension for three sets each. A linear progression was followed; 4 w with 12 repetitions maximum (RM), 4 w with 10 RM and 4 w with 8 RM, with progressively increased loads. Abdominal crunches and back extension were performed with 12–20 repetitions the whole period.
Endurance-training included one session of 7 min intervals at 85% of maximum heart rate (HRmax), and one session of 2 min intervals at >90% of HRmax per week. Rests between intervals included either slow cycling or complete rest. The number of 7 min intervals progressed from 3 to 4 after the second week, and from 4 to 5 after 6 weeks. The number of 2 min intervals progressed from 6 to 7 after the second week, and from 7 to 10 after 6 weeks.
Physical fitness and insulin sensitivity
The VO2max tests have been described in detail elsewhere7. Briefly, the tests started at a workload similar to the final load of an incremental test in which the relationship between work and oxygen uptake was established. After 1 min cycling, the workload was increased by 15 watts every 30 s until exhaustion. Success was defined as reaching an oxygen uptake plateau (<0.5 mL·kg−1·min−1 increase after 30 watt increased workload), respiratory exchange ratio (RER) values above 1.10, and blood lactate >7.0 mmol/L. The subjects abstained from training two days before the VO2max tests.
Euglycemic hyperinsulinemic clamp
Euglycemic hyperinsulinemic clamp7,28 was performed after overnight fast. Insulin sensitivity is reported as glucose infusion rate (GIR) relative to body weight (mg/kg/min) during the last 30 min of the clamp. A fixed dose of insulin 40 mU/m2·min−1 was infused, and glucose 200 mg/mL was adjusted to maintain plasma glucose levels at 5.0 mmol/L for 150 min. Whole blood glucose concentration was measured by a glucose oxidase method (YSI 2300, Yellow Springs, OH), and plasma glucose was calculated as whole blood glucose × 1.119. No physical exercise was performed within 3 days prior to the test.
Skeletal muscle biopsy sampling
Skeletal muscle biopsies (n = 154) were taken from m. vastus lateralis using a modified Bergström procedure29; (1) at rest, (2) immediately after 45 min sub-max test, and (3) 2 h after ended sub-max test, before as well as after the 12 w intervention. For some participants the 1st and 2nd biopsies were obtained from the same incision site, but from opposite angles. After sterilization, the subcutaneous and superfacial tissues were injected with Xylocain-adrenaline 10 mg/mL + 5 μg/mL, and when deemed necessary by the physician, the actual muscle tissue was injected with a non-adrenalin anesthetic (Lidokain, 10 mg/mL). A 6 mm (diameter) muscle biopsy needle (Pelomi, Albertslund, Denmark) was used with a 50 mL syringe for vacuum generation. Muscle biopsies were quickly rinsed in cold PBS and dissected on a cold aluminum plate to remove blood and subcutaneous adipose tissue before freezing.
Skeletal muscle lipidomics
Quantification of lipid classes in accurately weighed muscle biopsies, freeze-dried and stored at −80 °C. At the day of analysis the samples were thawed and homogenized in chloroform, methanol and water (65:35:0.4) using a motorized pellet pestle (Kontes; Vineland, NJ, USA). After centrifugation the supernatants were analyzed for lipid class content using an Agilent 1100 normal phase liquid chromatographic system coupled to an Evaporative Light Scattering Detector (ELSD). Separation of the neutral lipids was performed on a Chromolith Performance Si 100–4.6 mm HPLC column (Merck, Darmstadt, Germany) using a mixture of hexane, tert-butyl methyl ether and acetic acid (1000:50:0.25; vol) as mobile phase. The polar lipids were separated on an YMC PVA-SIL-NP 250 × 4.6 mm, 5 µm column using hexane, isopropanol, acetonitrile, chloroform, water, tert-butyl methyl ether and acetic acid mobile phase A (431:457:42:42:20:9:1.5; vol), and mobile phase B (333:507:42:42:70:7:0.15; vol) with gradient elution. Unknowns were calibrated against known standards form NU-CHEK-PREP, INC, MN, USA and reported as g/100 g sample. Standards used for PE were lipoid GMBH, PN: 585009-2120002-01/900, 1,2-diacyl-sn-glycero-3-phosphoethanolamine, and for PC were lipoid GMBH, PN: 579209-2120002-01/974, phosphatidylcholine from soybean.
Selected bundles of muscle biopsies were taken just after acute exercise before as well as after the training intervention (n = 18), and handled as previously described23. Briefly, the tissue was embedded in Durcupan, ultrathin sections of 60 nm were cut using an ultramicrotome from Leica (Vienna, Austria), and images were obtained using a Tecnai G2 electron microscope from FEI (Hillsboro, OR, USA). Point counting30 was performed at 6000‐fold magnification on 5 randomly selected areas, total of 400 μm2, from each subject using a 150 × 150 lattice. The hit points of lipid droplets/mitochondria were marked manually on blinded images, and counted. The percentage was estimated by the ratio of hit points/total points*100%.
RNA isolation, cDNA synthesis, TaqMan real-time RT-PCR and mRNA sequencing
Frozen biopsies were cooled in liquid nitrogen and crushed to powder by a pestle in a liquid nitrogen-cooled mortar. Frozen biopsies were transferred into 1 mL QIAzol Lysis Reagent (Qiagen, Hilden, Germany), and homogenized using TissueRuptor (Qiagen) at full speed for 15 sec, twice. Total RNA was isolated from the homogenate using miRNeasy Mini Kit (Qiagen). RNA integrity and concentration were determined using Agilent RNA 6000 Nano Chips on a Bioanalyzer 2100 (Agilent Technologies Inc, Santa Clara, CA). Using High‐Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster, CA), 200 ng of total RNA was converted to cDNA for TaqMan real‐time RT‐PCR. The cDNA reaction mixture was diluted in water and cDNA equivalent of 25 ng RNA used for each sample. Quantitative real‐time PCR was performed with reagents and instruments from Applied Biosystems in the 96‐well format using a 7900 HT Fast instrument and the SDS 2.3 software (Applied Biosystems). A predeveloped primer and probe set (TaqMan assays; Applied Biosystems) was used to analyze mRNA levels of Peroxisome Proliferator‐Activated Receptor Gamma, Coactivator 1 Alpha (PPARGC1A, Hs01016719_m1). Relative target mRNA expression levels were calculated as 2−ΔCt, and normalized to beta‐2 microglobulin (B2M, Hs00984230_m1). mRNA sequencing was performed using the Illumina HiSeq 2000 system (Illumina, San Diego, CA). cDNA sequenced reads alignment was carried out using Tophat v2.0.8. Reads counted by gene feature were performed by featureCounts in Rsubread 1.14.2 and analyzed using DEseq231.
Briefly, the ankle-to-neck MRI protocol included a 3D DIXON acquisition providing water and lipid quantification7. Water and lipid images were derived from multi-echo data using the vendor’s inline post processing, and further processed using the nordicICE software package (NordicNeuroLab, Bergen, Norway). We measured total body volume, and thigh muscle area (from 15 cm above the knee joint space).
Gene Set Enrichment Analysis (GSEA)
Pathway analyses were used to study the association between the PC:PE ratio and a large number of mRNAs, by correlating the PC:PE ratio with the expression of genes belonging to pre-defined, well-studied signaling pathways. We performed overlap tests based on hypergeometric means to explore pathways associated with the PC:PE ratio. The number of genes significantly correlated with the PC:PE ratio was compared to the total number of genes in a pathway using a hypergeometric distribution, which tests whether more genes in a pathway correlate with the PC:PE ratio than expected by chance. The P-values were calculated from a hypergeometric distribution in R using the syntax phyper (k-1, K, N-K, n), where K = genes in pathway; N = total number of genes tested; n = total number of significantly correlated genes; and k = significantly correlated genes in a pathway. The hypergeometric P-value is calculated as the probability of randomly drawing k or more successes from the population in n total draws. Sets of transcripts tested for enrichment were taken from the MSigDB database (KEGG gene sets; http://software.broadinstitute.org/gsea/msigdb/). We considered results as significant if the false discovery rate (FDR) was <0.0532.
Data were analysed using linear (mixed) regressions33 and presented as means ± SEM. We tested hypotheses concerning group differences at baseline, interaction effects between groups and time, the overall effect of exercise and group-specific exercise responses. Correlation analyses were performed using Pearson’s r or Spearman’s rank, as appropriate. Lipidomics data were lacking for one subject and the data was imputed using NIPALS33, with no influence on effect estimates or conclusions based on significance level. We evaluated normality and homogeneity by visual inspection of plots of residuals against fitted values. P-values were considered significant at α = 0.05, but should be interpreted with care due to the explorative nature of this study and the large number of tests performed. All data were analyzed using R (R Development Core Team, 2009).
The MyoGlu study adhered to the Declaration of Helsinki and was approved by the National Regional Committee for Medical and Health Research Ethics North, Tromsø, Oslo, Norway. Written informed consent was obtained from all participants prior to any study-related procedure.
Subject characteristics and responses to exercise
In the MyoGlu study the dysglycemic men had lower GIR and VO2max than the control men, but higher body weight, body volume, BMI, plasma glucose and insulin levels, leg press strength and larger thigh muscles as compared to control men at baseline (Table 1). Skeletal muscle levels of PC and PE and the PC:PE ratio were comparable between dysglycemic and control men (Fig. 2A).
Combined strength and endurance exercise for 12 w enhanced GIR, VO2max, leg press strength, and muscle size in both groups (Table 1). Furthermore, 12 w exercise caused appreciable alterations in skeletal muscle PC- and PE-levels, and in the PC:PE ratio (Fig. 2B). Both PC- and PE-levels were increased (21 and 42%, respectively), whereas the PC:PE ratio was reduced 16% in the whole cohort (Fig. 2B, black bars). Similar alterations were observed in both groups separately (Fig. 2B, grey and white bars).
Effect of acute exercise before and after 12 w of exercise intervention
Skeletal muscle PC- and PE-levels, and the PC:PE ratios were measured at rest, just after 45 min cycling at 70% of VO2max and after 2 h recovery following the acute exercise at baseline as well as after 12 w of exercise intervention (Fig. 3A–C). Whereas no clear patterns were observed for PC and PE levels individually, the PC:PE ratio was reduced in both groups after acute exercise (Fig. 3C). This reduction was observed at baseline in the untrained state, but not after 12 w exercise intervention in the trained state (Fig. 3C).
Skeletal muscle PC:PE ratio may predict insulin sensitivity
Potential mechanisms of altered PC- and PE-levels
We observed no concerted increase in phospholipid synthesizing pathways after 12 w exercise intervention, based on mRNA expression (Fig. 5). However, some enzyme mRNAs were responsive to exercise; eg. expression of the enzyme PCYT2 in the PE synthetic pathway and the enzyme CHPT1 in the PC synthetic pathway were increased, whereas the expression of the PEMT enzyme, responsible for synthesis of PC from PE, was reduced. We did not observe any consistent changes in gene expression within the glycerol phosphate pathway. Furthermore, the PC:PE ratio correlated with PEMT expression (Fig. 6) and PISD expression (baseline: ρ = −0.53, P = 0.007, delta: ρ = −0.53, P = 0.001). We did not observe any consistent changes in PC- and PE-related transcripts after acute exercise (data not shown).
Potential links between the PC:PE ratio and insulin sensitivity
Changes in the PC:PE ratio in response to 12 w exercise intervention correlated negatively with metabolic gene expression (i.e. oxidative phosphorylation) and mTOR, based on transcriptomic analyses (Table 2). In addition, changes in the PC:PE ratio in response to 12 w exercise intervention correlated with the percent area of mitochondria and lipid droplets as compared to total cell area based on EM pictures (Fig. 7C and D). mRNA levels of PGC1α (PPARGC1A), measured by RT-qPCR, correlated with the percent area of mitochondria (Fig. 7B), with a similar result using RNA-Seq to quantify PGC1α (ρ = 0.60, P = 0.008). The area of mitochondria increased from 3.4 to 5.4% after 12 w exercise intervention for all 18 men together (Fig. 7A). A similar increase was observed in both groups (Fig. 7A), although only the control group reached statistical significance (Fig. 7A). Additional information regarding the lipid droplets in skeletal muscles have been published elsewhere23.
Substantial evidence indicates that phospholipid composition is biologically important for several functions within the skeletal muscle linked to mitochondria, cell growth, contraction, exercise performance, and insulin sensitivity14. The main findings of our present investigation were: 1) the skeletal muscle PC:PE ratio may predict insulin sensitivity both at baseline and in response to long-term physical exercise; 2) the skeletal muscle PC:PE ratio is reduced in response to acute as well as long-term physical exercise; 3) there are several potential molecular links between the skeletal muscle PC:PE ratio and insulin sensitivity; mitochondrial function might be an important one.
Alterations in skeletal muscle PC and PE concentrations are related to synthesis and degradation. Synthesis of PC and PE involves several phospholipid synthesizing enzymes involved in the glycerolphosphate and Kennedy pathways (Fig. 5). We did not observe concerted changes on the mRNA level within these two pathways in response to the exercise intervention. However, we observed increased PCYT2 and CHPT1 mRNA levels (Fig. 5) in parallel to the increased PC and PE levels (Fig. 2). We also observed decreased PEMT mRNA levels in response to 12 w exercise intervention (Fig. 5) and striking correlations with the PC:PE ratio (Fig. 6). This might explain a reduction in the PC:PE ratio after exercise intervention, because PEMT is responsible for synthesis of PC from PE. This is in line with the observation that Pemt(−/−) mice have a reduced PC:PE ratio, increased mitochondrial activity, and high cellular ATP levels in skeletal muscle34.
Reduced skeletal muscle PC:PE ratio in response to 12 w exercise intervention was paralleled with increased GIR in our study (Fig. 4). GIR is a measurement of full-body insulin sensitivity, although approximately 80% variation in GIR might be explained by skeletal muscle insulin sensitivity28. Mitochondrial density and function in skeletal muscle may relate to exercise-induced increases in insulin sensitivity35. We observed that changes in skeletal muscle PC:PE ratio in response to the exercise intervention was negatively correlated with changes in skeletal muscle oxidative phosphorylation and mTOR signaling, based on transcriptomics (Table 2). Both these pathways are responsive to long-term exercise, and are associated with increased mitochondrial biogenesis, oxidative metabolism and insulin sensitivity36. Furthermore, changes in skeletal muscle PC:PE ratio in response to 12 w exercise intervention correlated negatively with changes in the percent area of mitochondria in muscle cells (Fig. 7). Moreover, mitochondria exhibit high abundance in skeletal muscle (Fig. 7), and mitochondrial membranes have a low PC:PE ratio (~1.2), at least compared to the sarcolemma (~2.1) and the ER/SR (~2.0) in rat m. vastus lateralis37. The inner mitochondrial membrane is most enriched with PE, and increased folding of the inner membrane might increase oxidative capacity14. Although we do not have direct measurements of membrane folding or enzyme activity, our data might imply that the skeletal muscle PC:PE ratio and GIR relate to the density, morphology, and function of mitochondria.
The PC:PE ratio in ER/SR membranes is increased in insulin-resistant compared to insulin-sensitive primary muscle cells38. Murine studies on skeletal muscle-specific knock-outs of PC- and PE-related enzymes have shown reduced PE synthesis, and increased PC:PE ratio in ER/SR membranes19,20,21. These alterations caused reduction in skeletal muscle mass, ER/SR Ca2+ ATPase (SERCA) activity, and exercise performance19,20,21. Because long-term exercise increases insulin sensitivity, skeletal muscle mass and exercise performance, these adaptations may be associated with alterations in ER/SR membranes. Other possible links between changes in skeletal muscle PC:PE ratio and changes in insulin sensitivity may be due to the role of PC and PE in plasma membrane integrity39, fluidity and lipid rafts10 with effects on insulin receptor kinetics11,12,13, cytokine-induced inflammation40,41,42, and glucose uptake19,20,21.
Whereas PC and PE did not respond to 45 min acute exercise at 70% VO2max in neither group, the PC:PE ratio was reduced after exercise in both groups at baseline but not after 12 w intervention (Fig. 3). These results apparently contrast those of Newsome et al.22. Although PC and PE exhibited marked group-dependent responses among athletes and subjects with T2DM or obesity, no changes were observed in the PC:PE ratio22. There are several possible reasons for these apparent differences: (a) whereas the exercise challenge was performed after overnight fast in Newsome et al.22, our participants received a standardized carbohydrate-rich meal prior to testing; (b) substrate utilization differs when testing at 50% of VO2max for 1.5 h22 compared to 70% of VO2max for 45 min, with increased carbohydrate oxidation at higher VO2max43,44; (c) the total duration between resting and recovery biopsies in Newsome et al.22 was 3 h and 30 min, as compared to 2 h and 45 min in our study, perhaps capturing different stages in time responses; (d) the between-group differences in Newsome et al.22 were substantially larger concerning training status and insulin sensitivity compared to our study. As of why the PC:PE ratio in our data only responded to acute exercise at baseline, but not after 12 w might be explained by long-term adaptations to exercise; such as differences in substrate oxidation43,44,22. Unfortunately, we do not have data from indirect calorimetry, and are thus unable to relate short-term responses in PC and PE to fat and carbohydrate oxidation.
Limitations in this study include extensive amounts of data based on mRNA expression. Thus, we can only speculate concerning flux of lipids between membranes and organelles. GIR was normalized to body weight in this study, which might be biased by changes in body composition. However, normalizing GIR to FFM, derived from body impedance measurements, did not affect the conclusions (not included).
In conclusion, the skeletal muscle PC:PE ratio are elevated in conditions with insulin resistance, and reduced with increased insulin sensitivity. The molecular link between the skeletal muscle PC:PE ratio and insulin sensitivity may involve several mechanisms related to cellular membrane alterations. A summary of the findings are presented in Fig. 8.
The datasets analysed during the current study are being made freely available in conjunction with a separate publication, but are available from the corresponding author on request.
Goodyear, L. J. & Kahn, B. B. Exercise, glucose transport, and insulin sensitivity. Annual review of medicine 49, 235–261, https://doi.org/10.1146/annurev.med.49.1.235 (1998).
Stuart, C. A., Shangraw, R. E., Prince, M. J., Peters, E. J. & Wolfe, R. R. Bed-rest-induced insulin resistance occurs primarily in muscle. Metabolism: clinical and experimental 37, 802–806 (1988).
Wilson, P. W., McGee, D. L. & Kannel, W. B. Obesity, very low density lipoproteins, and glucose intolerance over fourteen years: The Framingham Study. American journal of epidemiology 114, 697–704 (1981).
Helmrich, S. P., Ragland, D. R., Leung, R. W. & Paffenbarger, R. S. Jr. Physical activity and reduced occurrence of non-insulin-dependent diabetes mellitus. The New England journal of medicine 325, 147–152, https://doi.org/10.1056/nejm199107183250302 (1991).
Soman, V. R., Koivisto, V. A., Deibert, D., Felig, P. & DeFronzo, R. A. Increased insulin sensitivity and insulin binding to monocytes after physical training. The New England journal of medicine 301, 1200–1204, https://doi.org/10.1056/nejm197911293012203 (1979).
Borghouts, L. B. & Keizer, H. A. Exercise and insulin sensitivity: a review. International journal of sports medicine 21, 1–12, https://doi.org/10.1055/s-2000-8847 (2000).
Langleite, T. M. et al. Insulin sensitivity, body composition and adipose depots following 12 w combined endurance and strength training in dysglycemic and normoglycemic sedentary men. Archives of physiology and biochemistry, 1–13, https://doi.org/10.1080/13813455.2016.1202985 (2016).
Sigal, R. J. et al. Effects of aerobic training, resistance training, or both on glycemic control in type 2 diabetes: a randomized trial. Annals of internal medicine 147, 357–369 (2007).
Cuff, D. J. et al. Effective exercise modality to reduce insulin resistance in women with type 2 diabetes. Diabetes care 26, 2977–2982 (2003).
Saha, S., Anilkumar, A. A. & Mayor, S. GPI-anchored protein organization and dynamics at the cell surface. Journal of lipid research 57, 159–175, https://doi.org/10.1194/jlr.R062885 (2016).
Gorski, J., Zendzian-Piotrowska, M., de Jong, Y. F., Niklinska, W. & Glatz, J. F. Effect of endurance training on the phospholipid content of skeletal muscles in the rat. European journal of applied physiology and occupational physiology 79, 421–425, https://doi.org/10.1007/s004210050532 (1999).
Nadiv, O. et al. Elevated protein tyrosine phosphatase activity and increased membrane viscosity are associated with impaired activation of the insulin receptor kinase in old rats. The Biochemical journal 298(Pt 2), 443–450 (1994).
Pilch, P. F., Thompson, P. A. & Czech, M. P. Coordinate modulation of D-glucose transport activity and bilayer fluidity in plasma membranes derived from control and insulin-treated adipocytes. Proc Natl Acad Sci USA 77, 915–918 (1980).
Heden, T. D., Neufer, P. D. & Funai, K. Looking Beyond Structure: Membrane Phospholipids of Skeletal Muscle Mitochondria. Trends in endocrinology and metabolism: TEM 27, 553–562, https://doi.org/10.1016/j.tem.2016.05.007 (2016).
Borkman, M. et al. The relation between insulin sensitivity and the fatty-acid composition of skeletal-muscle phospholipids. The New England journal of medicine 328, 238–244, https://doi.org/10.1056/nejm199301283280404 (1993).
Pan, D. A. et al. Skeletal muscle membrane lipid composition is related to adiposity and insulin action. The Journal of clinical investigation 96, 2802–2808, https://doi.org/10.1172/jci118350 (1995).
Vessby, B., Tengblad, S. & Lithell, H. Insulin sensitivity is related to the fatty acid composition of serum lipids and skeletal muscle phospholipids in 70-year-old men. Diabetologia 37, 1044–1050 (1994).
Takagi, A. Lipid composition of sarcoplasmic reticulum of human skeletal muscle. Biochimica et biophysica acta 248, 12–20 (1971).
Funai, K. et al. Skeletal Muscle Phospholipid Metabolism Regulates Insulin Sensitivity and Contractile Function. Diabetes 65, 358–370, https://doi.org/10.2337/db15-0659 (2016).
Funai, K. et al. Muscle lipogenesis balances insulin sensitivity and strength through calcium signaling. The Journal of clinical investigation 123, 1229–1240, https://doi.org/10.1172/jci65726 (2013).
Selathurai, A. et al. The CDP-Ethanolamine Pathway Regulates Skeletal Muscle Diacylglycerol Content and Mitochondrial Biogenesis without Altering Insulin Sensitivity. Cell metabolism 21, 718–730, https://doi.org/10.1016/j.cmet.2015.04.001 (2015).
Newsom, S. A. et al. Skeletal muscle phosphatidylcholine and phosphatidylethanolamine are related to insulin sensitivity and respond to acute exercise in humans. Journal of applied physiology (Bethesda, Md.: 1985) 120, 1355–1363, https://doi.org/10.1152/japplphysiol.00664.2015 (2016).
Li, Y. et al. Subsarcolemmal lipid droplet responses to a combined endurance and strength exercise intervention. Physiological reports 2, https://doi.org/10.14814/phy2.12187 (2014).
Carlsen, M. H. et al. Evaluation of energy and dietary intake estimates from a food frequency questionnaire using independent energy expenditure measurement and weighed food records. Nutrition journal 9, 37, https://doi.org/10.1186/1475-2891-9-37 (2010).
Nes, M. et al. Accuracy of a quantitative food frequency questionnaire applied in elderly Norwegian women. European journal of clinical nutrition 46, 809–821 (1992).
Andersen, L. F. et al. Evaluation of a quantitative food frequency questionnaire used in a group of Norwegian adolescents. European journal of clinical nutrition 49, 543–554 (1995).
Andersen, L. F. et al. Evaluation of a food frequency questionnaire with weighed records, fatty acids, and alpha-tocopherol in adipose tissue and serum. American journal of epidemiology 150, 75–87 (1999).
DeFronzo, R. A., Tobin, J. D. & Andres, R. Glucose clamp technique: a method for quantifying insulin secretion and resistance. The American journal of physiology 237, E214–223 (1979).
Bergstrom, J. Percutaneous needle biopsy of skeletal muscle in physiological and clinical research. Scandinavian journal of clinical and laboratory investigation 35, 609–616 (1975).
Weibel, E. R. Stereological principles for morphometry in electron microscopic cytology. International review of cytology 26, 235–302 (1969).
Love, M. I., Huber, W. & Anders, S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome biology 15, 550, https://doi.org/10.1186/s13059-014-0550-8 (2014).
Benjamini, Y. Discovering the false discovery rate. Journal of the Royal Statistical Society: Series B (Statistical Methodology) 72, 405–416, https://doi.org/10.1111/j.1467-9868.2010.00746.x (2010).
Bates, D., Mächler, M., Bolker, B. & Walker, S. Fitting Linear Mixed-Effects Models Using lme4. 2015 67, 48, https://doi.org/10.18637/jss.v067.i01 (2015).
van der Veen, J. N., Lingrell, S., da Silva, R. P., Jacobs, R. L. & Vance, D. E. The concentration of phosphatidylethanolamine in mitochondria can modulate ATP production and glucose metabolism in mice. Diabetes 63, 2620–2630, https://doi.org/10.2337/db13-0993 (2014).
Hoeks, J. & Schrauwen, P. Muscle mitochondria and insulin resistance: a human perspective. Trends in endocrinology and metabolism: TEM 23, 444–450, https://doi.org/10.1016/j.tem.2012.05.007 (2012).
Laplante, M. & Sabatini, D. M. mTOR signaling in growth control and disease. Cell 149, 274–293, https://doi.org/10.1016/j.cell.2012.03.017 (2012).
Fiehn, W., Peter, J. B., Mead, J. F. & Gan-Elepano, M. Lipids and fatty acids of sarcolemma, sarcoplasmic reticulum, and mitochondria from rat skeletal muscle. The Journal of biological chemistry 246, 5617–5620 (1971).
Paran, C. W. et al. Reduced efficiency of sarcolipin-dependent respiration in myocytes from humans with severe obesity. Obesity (Silver Spring, Md.) 23, 1440–1449, https://doi.org/10.1002/oby.21123 (2015).
Li, Z. et al. The ratio of phosphatidylcholine to phosphatidylethanolamine influences membrane integrity and steatohepatitis. Cell metabolism 3, 321–331, https://doi.org/10.1016/j.cmet.2006.03.007 (2006).
Curry, F. E. Modulation of venular microvessel permeability by calcium influx into endothelial cells. FASEB journal: official publication of the Federation of American Societies for Experimental Biology 6, 2456–2466 (1992).
Kmiec, Z. Cooperation of liver cells in health and disease. Advances in anatomy, embryology, and cell biology 161, III-XIII, 1–151 (2001).
Mold, C. Effect of membrane phospholipids on activation of the alternative complement pathway. Journal of immunology (Baltimore, Md.: 1950) 143, 1663–1668 (1989).
Martin, W. H. III. Effects of acute and chronic exercise on fat metabolism. Exercise and sport sciences reviews 24, 203–231 (1996).
Romijn, J. A. et al. Regulation of endogenous fat and carbohydrate metabolism in relation to exercise intensity and duration. The American journal of physiology 265, E380–391 (1993).
Bakdash, J. Z. & Marusich, L. R. Repeated Measures Correlation. Frontiers in Psychology 8 (2017).
The MyoGlu trial is registered at ClinicalTrail.gov; NCT01803568. This work was supported by grants from the institute of Basic Medical Sciences, UiO, Johan Throne-Holst Foundation for Nutrition Research, Freia Medical Research Foundation, the “Functional Genomics” and “Infrastructure” programs of the Research Council of Norway and the Southeastern Regional Health Authorities and EU-financed FP7 project (NutriTech grant agreement no: 289511). We thank Anne Randi Enget, Ansgar Heck and Birgitte Nellemann for taking the biopsies, and Tor I Gloppen, Torstein Dalen, Håvard Moen, Marius A Dahl, Guro Grøthe, Egil Johansen, Katrine A Krog, Øyvind Skattebo, Daniel S Tangen, Kristoffer K Jensen, Hans K Stadheim, and Eirin Rise for conducting the human strength and endurance intervention (MyoGlu) under supervision of Professor Jørgen Jensen. Sequencing was performed by PhD Gregor Gilfilan at the Norwegian Sequencing Centre (www.sequencing.uio.no) supported by the Research Council of Norway and the South-eastern Regional Health Authorities.
The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the reported research except for A.A., T.E.G. and C.A.D. who are associated to Vitas Ltd. A.A. and T.E.G. are employed in Vitas. T.E.G. and C.A.D. are cofounders and stock owners, and C.A.D. is a board member and a consultant for Vitas Ltd.
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Lee, S., Norheim, F., Gulseth, H.L. et al. Skeletal muscle phosphatidylcholine and phosphatidylethanolamine respond to exercise and influence insulin sensitivity in men. Sci Rep 8, 6531 (2018). https://doi.org/10.1038/s41598-018-24976-x
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