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The wavelength of the incident light determines the primary charge separation pathway in Photosystem II

Scientific Reportsvolume 8, Article number: 2837 (2018) | Download Citation

Abstract

Charge separation is a key component of the reactions cascade of photosynthesis, by which solar energy is converted to chemical energy. From this photochemical reaction, two radicals of opposite charge are formed, a highly reducing anion and a highly oxidising cation. We have previously proposed that the cation after far-red light excitation is located on a component different from PD1, which is the location of the primary electron hole after visible light excitation. Here, we attempt to provide further insight into the location of the primary charge separation upon far-red light excitation of PS II, using the EPR signal of the spin polarized 3P680 as a probe. We demonstrate that, under far-red light illumination, the spin polarized 3P680 is not formed, despite the primary charge separation still occurring at these conditions. We propose that this is because under far-red light excitation, the primary electron hole is localized on ChlD1, rather than on PD1. The fact that identical samples have demonstrated charge separation upon both far-red and visible light excitation supports our hypothesis that two pathways for primary charge separation exist in parallel in PS II reaction centres. These pathways are excited and activated dependent of the wavelength applied.

Introduction

Several billion years ago, the evolution of photosynthetic organisms capable of splitting water into molecular oxygen (O2), protons [H+], and electrons (e) with the mere absorption of sunlight, completely altered the course of evolution of life1,2. The complex photosynthetic reactions in these organisms (plants, algae and cyanobacteria), allow them to take in carbon dioxide (CO2), water and light energy to produce carbohydrates and O2, vital products for life sustainment. These light-dependent reactions take place in two multi-protein membrane complexes known as Photosystem II (PS II)3 and Photosystem I (PS I)4. PSII absorbs photons to use their energy to oxidize water, thereby supplying PS I with electrons to be used in carbon fixation5,6.

In PS II, absorption of a photon first results in excitation of photosynthetic pigments and very rapidly thereafter in the primary charge separation reaction between the primary donor of PS II, commonly referred to as P680, and the first electron acceptor, pheophytin (Pheo). In a series of subsequent electron transfer reactions, electrons are transferred from water (that is split into O2 when oxidized) to the first and second quinone electron acceptors, QA and QB7,8. Unlike anoxygenic phototrophic bacteria, which contain bacteriochlorophylls that can drive anoxygenic photosynthesis at wavelengths longer than 900 nm, oxygenic photosynthesis was thought to be limited to wavelengths below ca 690 nm, also known as the red drop phenomenon as defined by9. The reason behind this difference was attributed to the high energy required for water oxidation in oxygenic photosynthesis, where O2 is released as a by-product. Lower energy photons above ca 690 nm were assumed to be unable to generate a redox potential that is high enough to drive water oxidation, while concomitantly retaining a negative excited state redox potential on the acceptor side required for the reduction of the primary electron acceptor10.

The notion that oxygenic photosynthesis can only be driven by photons with a maximum absorbance at or below ~690 nm involving Chl a, drastically changed with the discovery of the cyanobacterium Acaryochloris marina11,12,13. This photosynthetic cyanobacterium has ~95% of its Chl a substituted by Chl d, which is red-shifted with an absorbance maximum of ~710 nm. Despite this, A. marina is still able to oxidize water14,15. This results in efficient photochemistry taking place in a submarine ecosystem deficient in visible light, but largely penetrated by far-red light16,17,18. Thus, it is clear that photosynthetic water oxidation in nature can occur also with photons of energy lower than 690 nm. Interestingly, this also holds for PS II in green algae19 and higher plants20,21 containing a Chl a/b antennae without any Chl d. Spectroscopic investigation of the different electron transfer reactions in PS II, defined the far-red limit for water-oxidizing photochemistry in PS II to 780 nm (single flash) up to 800 nm (photoaccumulation)22. Based on these findings, an alternative charge separation pathway was proposed to occur upon far-red light excitation. When this pathway is activated, a state denoted X* that is lower in energy compared to P680* (formed in visible light) is formed in PS II. Despite its lower energy, X* is able to trigger the charge separation, reduce Pheo and oxidize tyrosine Z (YZ).

This is an important evolutionary finding as it has redefined the required threshold energy for oxygenic photosynthesis, since X* apparently works with an energetic threshold which is lower than P680*22. The existence of such a state was suggested previously, but never shown to induce charge separation at room temperature or at wavelengths as high as 800 nm. Although oxygen evolution and variable chl a fluorescence have been observed in sunflower (Helianthus annuus) and bean (Phaseolus vulgaris) leaves using wavelengths up to 780 nm21, the maximum long wavelength with which successful charge separation was achieved was 730 nm, as demonstrated by spectral hole-burning experiments on PS II core complexes at cryogenic temperatures (1.5 K)23,24. This range has now been extended to ca 780–800 nm where complete water oxidation can be achieved at room temperature in different PS II preparations22.

Mokvist et al., 2014 provided further insight into the electron transfer reactions in PS II, induced by far-red photochemistry25. It was shown that at 5 K, YZ was the preferred secondary electron donor in far-red light. This is different from the situation with visible light where the Cyt b559/ChlZ/CarD2 secondary pathway was equally important25.

This observation led to the proposal of a different first stable charge pair denoted as Px+ QA being formed under far-red light, as compared to the normal PD1+ QA under visible light excitation. The proposed electron hole in Px+ was suggested to be residing on the ChlD1 molecule in the PS II reaction centre at 5 K. The observation also further supported the proposed existence of a low-energy threshold charge separation pathway, where the primary donor is ChlD1 rather than PD125. (See Fig. 1 which shows the redox active components in the PS II reaction centre from ref.26).

Figure 1
Figure 1

Structural representation of the redox active components in the PS II reaction center.

In this study, we have used the spin polarized triplet EPR signal from 3P680, to further probe the photochemistry following visible or far-red illumination. Our results strengthen the hypothesis that the stable cation formed by the charge separation resides at ChlD1 when the excitation energy is provided by long wavelength (far-red) light, rather than on PD1 of the PD1/ PD2 Chl pair, which is the normal situation in visible light excitation.

Results

Functionality of over-reduced PS II

The experimental protocol to achieve reliable and quantitative double reduction of QA in PS II enriched membranes of our type (so called BBY particles), is complicated and has several potential pitfalls. The double reduction protocol involves reduction with sodium dithionite using benzyl viologen as mediator for several hours and demands exclusive handling under anaerobic and dark conditions27,28,29. Also the re-oxidation protocol is difficult and could potentially lead to sample damage27. It was therefore important to test for the integrity of the critical electron transfer reactions both after double reduction and after the re-oxidation of the samples used here.

Status of the charge separation reaction

The most important reaction in the current study is the primary charge separation reaction. In PS II centres with QAH2 (double reduced and protonated QA) or samples lacking QA, the primary charge separation can be followed by photo-accumulation of the Pheo radical under reducing conditions30,31,32. This reaction is also known to be functional using far-red illumination22.

Figure 2 shows the EPR signal from the Pheo radical in PS II enriched membranes where QA was doubly reduced. The radical EPR spectrum formed by either visible or far-red (732 nm) light was 13G wide with g = 2.003530,31,32. Both parameters are indicative that the signal originates from the Pheo radical. Thus, the mere formation of the signal is an indication that PS II was able to perform the charge separation reaction despite being exposed to the dithionite/benzyl viologen reduction protocol. The size of the EPR spectrum from Pheo can be compared to the size of the EPR spectrum from the YD radical in the corresponding intact PSII sample (non-reduced) that amounts to 1 radical/PSII reaction center33,34. In this particular experiment, exposure to white light resulted in Pheo radical formation in 70% of PS II, while the illumination at 732 nm resulted in Pheo radical formation in 55% of PS II. We can thus conclude that ≥70% of the PS II centres can perform the primary charge separation after the reduction treatment. In addition, it seems that 732 nm is nearly as efficient as white light, the small difference probably reflecting the weaker light source used at 732 nm.

Figure 2
Figure 2

EPR signal of the Pheo radical in doubly reduced PS II membranes, after continuous illumination with white (black) and far-red at 732 nm light (red), at 20 °C, for 6 and 10  min respectively. EPR conditions were as follows: microwave power 1.27 μW, microwave frequency 9.139 GHz, modulation amplitude 3.5 G, temperature, 15 K.

Status of the Mn-cluster

It is generally thought that extensive reduction of PS II results in inactivation of the OEC through removal of the CaMn4—cluster. This also holds for our samples that showed typical characteristics of samples lacking the CaMn4-cluster. After the re-oxidation procedure, no O2 evolution could be detected. Corroborating this, neither the Split S1 EPR signal nor the S2 state multiline EPR signal, both involving a functional CaMn4-cluster, could be detected (EPR spectra not shown).

Status of Tyrosine-D oxidation and the quinone acceptors QA and QB

Chemical double reduction of QA is most probably followed by protonation, forming QAH227,28,29. Presumably this species could leave the QA-binding pocket irreversibly during the very long double reduction procedure. However, this does not seem to be the case, at least not in the majority of the PSII centres. In the re-oxidized samples, YD could be formed in a major fraction of the PS II centres (>55%) (Fig. 3, red trace). YD oxidation involves both primary photochemistry in PSII (charge separation between P680 and Pheophytin) and the transfer of, at least, one electron to the quinones on the acceptor side of PS II. Thus the formation of YD unequivocally shows both that charge separation worked (Figs 2 and 3, red trace) and that QA remained bound in a majority of the samples despite the harsh double reduction/re-oxidation protocols.

Figure 3
Figure 3

(a) The radical EPR spectrum of maximum oxidized YD (black) as measured from an untreated PS II sample. (b) The radical EPR spectrum of oxidized YD (red) as measured from a similarly concentrated PSII sample where QA was re-oxidized after double reduction with sodium dithionite/benzyl viologen (see Materials section). EPR conditions: microwave power 1.3 μW, microwave frequency 9.28 GHz, modulation amplitude 3.5 G, temperature 15 K.

We also performed flash induced fluorescence measurements in the re-oxidized samples. Our results show that a large fraction of PSII was able also to perform secondary electron transfer. After the flash, the immediate fluorescence induction is indicative of reduction of QA, when bound to its binding pocket. QA then decays with different kinetics dependent on the integrity of PSII. The dominating intermediate decay phase in our samples (calculated half-life = 13 ms) (Fig. 4, black trace), is similar to the YZ QA recombination phase observed in for example Tris-washed PS II membranes35. In Tris-washed PSII membranes the OEC is absent, corroborating our conclusion that our samples after double reduction and re-oxidation had lost the CaMn4-cluster.

Figure 4
Figure 4

Flash induced fluorescence decay kinetics from the re-oxidized double reduced PS II membrane samples. Traces indicate the fluorescence yield changes after a single flash in the presence (red) or in the absence (black) of DCMU.

We can also conclude that, not surprisingly, a substantial amount of QA remained reduced also after the re-oxidation protocol. This is shown by the high F0 (0.63) in our fluorescence measurement (Fig. 4, black trace). Furthermore, there seems to remain very few PS II centres with bound QB after the re-oxidation procedure. This can be concluded from comparison of the kinetic traces in the presence and absence of the inhibitor DCMU. Addition of DCMU, which inhibits electron transfer from QA to QB, removed a small fast component from the fluorescence decay curve (Fig. 4, red trace). This fast phase most likely reflects electron transfer from QA to a remaining bound QB. Normally this fast decay phase dominates in PSII and its small amplitude in our samples clearly indicates that there is very little QB remaining after the double reduction/re-oxidation treatments. The rest of the decay is similar to the decay observed in presence of DCMU and again reflects YZ QA recombination.

Induction of the spin polarized 3P680 under white and far-red light excitation

The spin polarized triplet state 3P680 is a useful probe to the physical environment and photochemistry of P680 and the primary radical pair in PSII36. In intact PSII, 3P680 is not observable with EPR spectroscopy37. However, when QA is either removed (in for example the D1/D2/Cyt b 559 preparation)37,38 or double reduced either by chemical treatment27,28,29 or extensive illumination under anaerobic conditions39, the spin polarized EPR signal from 3P680, can be observed as a result of recombination of the P680+ Pheo charge pair.

Here, we have investigated the formation of 3P680 in samples where QA has been chemically double reduced. Figure 5 depicts the “light minus dark” difference EPR spectra from the spin-polarized triplet state of 3P680 obtained from PSII membranes with double reduced QA, when illuminated by continuous white light (spectrum (a)) at 5 K. Our chemical reduction procedure converts QA to QA2−, which is then protonated to form QAH227,29. After excitation with light, no forward electron transfer from Pheo is possible. Instead, the charge-separated state P680+ Pheo will decay through recombination. However, it is long-lived enough to allow spin dephasing, thereby allowing formation of the triplet form of the radical pair, the so called spin-polarized 3P680 state as follows36,40:

$${{\rm{P}}}_{{\rm{680}}}{\rm{Pheo}}\iff {{{\rm{P}}}_{{\rm{680}}}}^{\ast }{\rm{Pheo}}\iff [{{{\rm{P}}}_{{\rm{680}}}}^{+}{{\rm{Pheo}}}^{-}]{\rm{Spin}}\,{\rm{dephasing}}\,\& \,{\rm{recombination}}{\Rightarrow }^{{\rm{3}}}[{{\rm{P}}}_{{\rm{680}}}]$$

Hence, at low temperatures (5 K), the triplet state of the primary donor, 3P680, is formed with high yield27,29,38,41.

Figure 5
Figure 5

Light minus dark difference EPR spectra showing the formation (or not) of the spin-polarized 3P680 in PS II samples after reduction with sodium dithionite and benzyl viologen. The light spectra were acquired under continuous illumination at 5 K with (a) white light and (b) far-red light (732 nm) EPR conditions: microwave power 63 μW, microwave frequency 9.139 GHz, modulation amplitude 30 G, temperature, 5 K.

From orientation studies of the 3P680 EPR signal it was concluded that it is localized on a Chl molecule (unidentified at that time) most likely to be ChlD1 (in the present structural nomenclature, see Fig. 1)38,42,43. It is known that the product of the primary charge separation in visible light is PD1+ Pheo. Therefore, the localization of the 3P680 on another Chl molecule than PD1 is unexpected. The phenomenon has been explained by two alternative mechanisms. Either the triplet state migrates from PD1 to ChlD1 after its formation or it is the Chl cation (PD1+) that moves to ChlD1 (forming ChlD1+) prior to 3P680 formation (38 and refs. therein).

As shown in Fig. 5, spectrum (a), the characteristic spin-polarized EPR signal from 3P680 was observed under white light illumination. The spectrum is in agreement with previously published EPR spectra of 3P68027,29,36,37,38. The triplet EPR spectrum depicts a low-field to high-field AEEAAE (Absorption = A, Emission = E) polarization pattern spanning ~650 G. X, Y and Z (D > 0, E < 0) indicate the field positions of the respective canonical orientations for the zero field splitting tensor of the triplet state.

We have also intensively searched for the formation of 3P680 in an identical sample after illumination in the EPR cavity with far-red light. Interestingly, as depicted in Fig. 5, spectrum (b), no spin polarized EPR signal from 3P680 could be detected under these conditions. This obvious difference between visible and far-red light excitation is intriguing as our control measurements clearly indicate that: i) our samples were indeed able to form 3P680 in visible light (Fig. 5, spectrum a) and ii) were indeed able to perform charge separation and a multitude of electron transfer reactions on both the donor and the acceptor side of PS II in both visible and far-red light (Figs 24).

Accumulated Spectra of spin polarized 3P680

It is possible that the lack of the 3P680 EPR signal under far-red light reflects the complete absence of 3P680 formation. However, it could also be caused by a very fast 3P680 decaying species not detectable under standard CW EPR conditions. To test this, we therefore accumulated flash—associated spectra at 532 nm, 610 nm, 689 nm, 730 nm, 750 nm and 790 nm at 5 K, recorded with a flash frequency of 5 Hz. Figure 6 shows the flash-associated transients recorded in the low-field region of the 3P680 spectrum (2850–3150 G) upon 532 nm (a), 610 nm (b), 689 nm (c) and 730 nm (d) laser flash excitation. The intensity of the spin-polarized 3P680 features is high under 532 nm and 610 nm excitation and decreased by more than 50% under 689 nm excitation. Ultimately, there is no EPR signal from 3P680 detected even with our fastest available time resolution and after extensive illumination with far-red laser flash excitation (730–790 nm) (Fig. 7). It is also important to point out that the decay kinetics of respectively the X and Z peaks are identical irrespective of the induction wavelength between 532 nm and 689 nm (Fig. 7a,b). The decay kinetics are, however, faster for the X peak than for the Z peak similar to earlier observations36. At ≥730 nm no peaks were observed.

Figure 6
Figure 6

Flash-associated transient 3D spectra of spin polarized 3P680 at 532 nm (40 mJ) (a), 610 nm (35 mJ) (b), 689 nm (35 mJ) (c) and 730 nm (20 mJ) (d) (numbers in parentheses denote the laser flash power), laser flash excitation. Each spectrum represents the accumulation of 1000 transients at 32 field positions (low-field peaks) respectively, with a delay time before flash application of 1 ms, at 5 K. The EPR signal was detected ~50 μs after laser excitation with a detection time window of 20 ms. EPR conditions were as follow: microwave power 63 μW, microwave frequency 9.139 GHz, receiver time constant 0.08 ms, modulation amplitude 35 G, temperature, 5 K.

Figure 7
Figure 7

Spin polarized 3P680 flash-associated transient decay traces upon 532 nm (black), 610 nm (red), 650 nm (blue), 689 nm (pink), 730 nm (purple), 750 nm (orange) and 790 nm (green) flash excitation recorded at fixed field positions X (a) and Z (b) of the low field region of the 3P680 EPR spectrum (see inserts and Fig. 4). The traces are the result of 1000 accumulated transients at 5 K. EPR conditions as in Fig. 5.

Discussion

The understanding of how low energy excited states contribute to PSII photochemistry continues to be a challenging task44. This is especially true if specific charge transfer bands, not detectable by conventional spectroscopy, are responsible for this type of photochemistry44,45. In this study we are analysing these phenomena, by using EPR based, comparative product analysis (see also ref.45) using the characteristic EPR signal of spin polarized 3P680 as a probe for the primary charge separation at low temperatures.

In our first publication we showed, for the first time, that PSII operates much further to the far-red region (by 100 nm) than was believed before. We have shown that efficient secondary electron transfer reactions take place under these conditions, including turnover of the OEC, reduction of the primary and secondary quinones, oxidation of the secondary donor TyrZ and reduction of the primary donor, Phe, using far-red (up to ≥800 nm) photons. This was an interesting observation and in an earlier study we proposed an alternative charge separation reaction under far-red conditions involving a low energy primary donor, denoted X*, which was able to drive both Pheo reduction and YZ oxidation22.

This suggestion was further substantiated in a study of low temperature (5 K) electron transfer on the donor side of PS II. To elaborate, we found that far-red light preferentially promoted electron transfer from YZ to the primary donor. In contrast, visible light promoted almost equal electron transfer from either YZ or the Cyt b559/ChlZ/CarD2 secondary pathway. The partition ratio between the two electron donor pathways was thus wavelength dependent, YZ being the better donor after far-red excitation25.

An important implication of this work, discussed in22,25 is that the far-red photochemistry is triggered by weakly absorbing charge transfer states among the core pigments in PSII and not by the well-known bands at 680 nm (PD1) or 684 nm (ChlD1).

In our present manuscript, we are getting even closer to the primary charge pair, P680+ Pheo, showing that the primary donor is indeed different. Thus, our, step wise refined, product analysis technique provides a unique approach to study the far-red photochemistry in PSII and to cast further light on this phenomenon by studying the primary electron donor in PS II, P680, directly. Our result is surprising. As described previously, the spin polarized EPR signal from 3P680 can be observed from PS II centres where QA has been doubly reduced (or it is absent). It has earlier been shown that its formation using white light is almost quantitative27,36. In our hands, the 3P680 EPR signal is large and fairly consistent in amplitude, when PS II is exposed to light in the visible region. However, in the far-red, at wavelengths ≥730 nm the illumination does not induce any observable 3P680 EPR signal at all.

There can be several reasons for the above phenomenon. To find the answers and formulate our hypothesis, certain questions must be addressed: (i) Is the electron hole on the primary donor not forming at all under far-red light illumination? (ii) If it was formed in far-red light, would the 3P680 decay too fast and escape detection? (iii) Do we trigger an alternative charge separation pathway by far-red light, hence creating a different, low energy, state as suggested in ref.20? (iv) Is the cation located at a different Chl if compared to the visible light as hypothesized in ref.23? If yes, then the recombination between the primary donor and Pheo occurs too fast to allow 3P680 formation after far-red illumination.

First, we can exclude that this particular type of PS II sample (sodium dithionite/benzyl viologen reduced) is not able to carry out any charge separation in the far-red at all, since we in fact can photoaccumulate the Pheo radical in the majority of PS II under illumination with 730 nm light. Therefore, an electron hole on the P680 entity is formed after excitation by far-red light. In that case, recombination between Pheo- and P680+ will take place, either via or without the formation of 3P680.

Second, in far-red light we observe no spin polarized 3P680 EPR signal. However, in the event where 3P680 is anyway formed by the far-red light, it is possible that it decays faster than we can detect it, in contrast to the situation in the visible part of the spectrum. An attempt has been made to investigate this possibility further by our kinetic measurements of 3P680 induced by different light wavelengths. Here we report identical decay kinetics between the different light regimes where we could observe the 3P680 in the visible light spectrum. However, we were not able to observe any 3P680 formation above 700 nm, despite our sub-millisecond time resolution. We argue that there is little reason to suggest that 3P680 formed by far-red light should decay too fast to allow its detection. Instead, we propose that the far-red light illumination, although it efficiently drives charge separation and creation of a primary radical pair [P680+ Phe], does not result in the formation of the spin polarized EPR signal from 3P680. Since Pheo is the same in both cases, it is the nature of the Chl cation, (Chl+) that differs after excitation with visible or far-red light.

This observation also has important implications with respect to the earlier orientation studies of the spin-polarized 3P680 EPR signal formed by visible light (see above)38,42,43. From our results, we can rule out that the triplet state is formed after migration of the cation from one monomeric Chl to another (from PD1 to ChlD1). In this case we would have observed the 3P680 EPR signal under both illumination regimes since the triplet would have been formed from the same ChlD1+ Pheo charge pair. Instead, detection of the signal under visible light only strongly indicates involvement of different charge pairs under the two illumination regimes with the triplet state only being formed by the PD1+ Pheo charge pair recombination.

Third, we have recently demonstrated preferential electron transfer from YZ in comparison to very weak electron transfer from the Cyt b559/ChlZ/CarD2 side donors under far-red light illumination at 5 K25. To explain this, we hypothesized the existence of an alternative charge separation pathway under far-red light involving the oxidized primary donor after charge separation residing on ChlD1 rather than PD125. Thus, we suggest that upon far-red excitation, a new pathway is activated. We denote this as the the ChlD1 pathway (Fig. 1). Here, a lower energy excited state [ChlD1 Pheo]* is formed, most probably corresponding to the proposed X* in ref.20, which would then lead to a different radical pair after the charge separation, [ChlD1+ Pheo] (Fig. 1).

Lastly, the lack of observable triplet signal implies that the recombination from Pheo is too fast to allow spin dephasing. This suggests that the electron hole is presumably residing on ChlD1 instead of PD1. ChlD1 is much closer to Pheo than PD1 (5 Å vs 8.1 Å)26. This shorter distance would probably facilitate faster recombination of the charge pair ChlD1+ Pheo. Hence, we propose that the far-red light preferentially drives charge separation to Pheo from ChlD1 in the reaction center (Fig. 1). Since ChlD1+ and PheoD1 are in close proximity, we propose that recombination occurs faster than between the radical pair [PD1+ Pheo] found in visible light. Thus, the [ChlD1+ Pheo] radical pair probably recombines back to the ground state prior to spin dephasing can occur. Consequently no spin polarized 3P680 is formed at any of the PSII centres.

It is generally thought that at low temperatures the cation in the primary radical pair is stabilized on PD1 (Fig. 1)40,46,47,48,49,50, which is not far from neither YZ nor the Cyt b559/ChlZ/CarD2 secondary pathway. Therefore they compete, and both are oxidized in substantial yield at very low temperatures25. Contrary, the localization of the primary electron hole on ChlD1 rather than PD1 under far-red light was discussed in ref.23. It was proposed that with the formation of the primary electron hole on ChlD1, the location of ChlD1 being relatively closer to YZ but much more distant from the Cyt b559/ChlZ/CarD2 secondary pathway, could explain at least qualitatively, the preference for oxidation of YZ over Cyt b559/ChlZ/CarD2.

The existence of parallel charge separation pathways in PSII under different light regimes and under physiological and cryogenic conditions has been suggested and discussed before50,51,52,53,54,55. In 2009 Thapper et al.22 suggested the formation of a low-energy state denoted X* in PS II after far-red light excitation at room temperature. Although functioning at a lower energetic threshold compared to P680*, it was shown to effectively trigger the charge separation, reduce Pheo and oxidize YZ up to ~800 nm. More recently, transient absorption spectroscopy at 77 K, suggested the existence of two different charge separation pathways in PS II50. These were dependent on the light induced protein configuration in PSII core complexes. In one path, denoted the ChlD1 path, the charge separation events were proposed to involve an interaction between ChlD1 and Pheo, and follow the sequence (ChlD1 Pheo)*  (ChlD1+ Pheo)  (PD1+ Pheo). In the second pathway, denoted the PD1 path, a charge transfer state PD1 PD2 is excited and the charge separation events were suggested to follow an alternative sequence (PD1 PD2 Pheo)*  (PD1+ PD2 ChlD1)  (PD1+ PD2 Pheo)52,54,55. The proposal that the two pathways are actually of an antagonistic nature and the effectiveness of each of the pathways essentially depends on recognition of energetic disorder, fits with our observations.

When a low energy photon in the far-red is used for excitation, the subsequent energetic disorder causes the preferential excitation to be strongly localized at ChlD1, thus allowing for charge separation to take place with the first charge separated state being ChlD1+Pheo. This localized state should be energetically lower than the PD1 PD2 charge transfer state, which is in agreement with the results of the present study. Thus, we propose that two pathways for primary charge separation exist in parallel in PS II reaction centres and their excitation and activation is wavelength dependent (Fig. 1).

Concluding Remarks

Here, we have provided further insight into the location of the primary charge separation upon far-red light excitation of PS II, using the EPR signal of the spin polarized 3P680 as a probe. We demonstrate that, under far-red light illumination, the spin polarized 3P680 is not formed, although we show that the primary charge separation is still occurring at these conditions. We propose that this is because under far-red light excitation, the primary electron hole is localized on ChlD1, rather than on PD1 (Fig. 1). The formation of the lower energy excited state ChlD1 Pheo* by far-red light, leads to the [ChlD1+ Pheo] primary charge pair. The close proximity of these two cofactors, allows fast recombination to the ground state before spin dephasing can occur, hence the 3P680 cannot be formed.

The fact that identical samples have demonstrated charge separation upon both far-red and visible light excitation further supports our hypothesis that two pathways for primary charge separation exist in parallel in PS II reaction centres. They are excited and activated dependent of the wavelength applied. In the visible part of the spectrum the first product of the charge separation is normally considered to be PD1+ Pheo.

In stark contrast, we hypothesize that far-red illumination results in the ChlD1+ Pheo radical pair. The latter recombines without formation of the 3P680, thereby explaining the results in the present contribution. When far-red light is applied to intact PS II however, the oxidizing electron hole, presumably ChlD1+, preferentially drives electron transfer from YZ and consequently the CaMn4 cluster. As described earlier, the Yz-CaMn4 pathway is preferred over the Cyt b559/ChlZ/CarD2 pathway at low temperatures due to the favourable location of ChlD1 vs YZ over ChlZ21.

Materials and Methods

PSII membrane preparation

Spinach (Spinacia oleracia) was grown hydroponically as described previously at 20 °C under cool white fluorescent light (Osram Powerstar HQI-400W/DV dysprosium lamp, intensity 300 μEm−2s−1), with light-dark periods of 12 h33. Oxygen evolving PSII enriched membranes (BBY-type) were prepared according to previously published procedures56,57. The membrane particles were re-suspended in a final buffer containing 400 mM Sucrose, 15 mM NaCl, 3 mM MgCl2 and 25 mM MES-NaOH pH 6.1, and frozen as beads at −80 °C, at a Chl concentration of 6 mg/ml.


Chemical reduction and re-oxidation of PS II

In order to obtain reaction centers with doubly reduced QA, PS II membranes were exposed to reducing conditions as described in27,28 with modifications as in29. Specifically, upon addition of 40 mM sodium dithionite, 100 μM benzyl viologen and 3 mM EDTA under anaerobic conditions, dark-adapted PS II membranes at a Chl concentration of 6 mg/ml, were incubated in the dark for 5 hrs to achieve the double reduction of QA to QAH2. All incubations were carried out in argon flushed EPR tubes. Upon completion of the incubation time, the EPR samples were frozen within 1 sec in a 200 K dry ice/ethanol bath and subsequently transferred into N2(l) before the measurements.

“Reversed” samples where QAH2 was re-oxidized, were prepared according to previously published procedures27. The doubly reduced samples were initially washed three times (15 000 × g, 15 min cycle) with argon flushed buffer containing 400 mM Sucrose, 15 mM NaCl, 3 mM MgCl2 and 25 mM MES-NaOH pH 6.1, to remove sodium dithionite and benzyl viologen. They were then re-oxidized with 5 mM K3Fe(CN)6, which was subsequently removed by repeating the washing procedure three times with final buffer, as described above. All washing steps were performed in complete darkness and at 4 °C.


Illumination procedures

The spin polarized 3P680 EPR signal was induced by illumination of the samples in the EPR cavity with white or far-red light at 5 K. For continuous illumination, white light, filtered through a 5 cm-thick copper sulphate solution heat filter, was provided by an 800 W halogen projector lamp. Far-red light illumination was achieved by the use of a custom made LED module, emitting continuous light centred at 732 nm. Appropriate long pass filters were used so as to ensure blocking of transmission of any stray UV light (CC4) as well as transmission of light <725 nm (RG9). Transient formation and accumulation of the spin polarized 3P680 EPR signal was studied by applying laser flashes into the EPR cavity, from a Spectra Physics PRO-290 Q-switched Nd:YAG laser (6 ns flash, 5 Hz flash frequency) equipped with a Spectra Physics Quanta Ray MOPO 730 optical parametric oscillator.

The Split S1 signal was obtained by white or far-red continuous wave light illumination at 5 K, as in58.

For the measurements of the Pheo radical, doubly reduced PS II membranes (6.0 mg Chl/ml) were subjected to white or far-red (732 nm) continuous wave light illumination as described earlier30,31,32, at 20 °C, for 6 and 10 minutes respectively at room temperature. Thereafter, the samples were frozen within 1 sec in a 200 K dry ice/ethanol bath and subsequently transferred into N2(l) before the measurements. The size of the EPR spectrum of the formed Pheo radical was compared by double integration to the size of the EPR spectrum from YD in a corresponding sample prior to double reduction, allowing us to quantify the function of the primary charge separation in the double reduced samples.


Fluorescence measurements

Flash-induced fluorescence decay measurements were performed at room temperature at a sample concentration of 20 µg Chl/ml. PS II membranes were dark adapted for 5 min and measurements were performed in the presence or absence of 20 µM DCMU. The variable fluorescence decay traces were recorded with a FL3000 double modulated fluorometer (PSI Photon Systems Instruments, Czech Republic) according to59. The first data point was taken 100 μs after the actinic flash. Measuring flashes were then applied logarithmically eight times per decade in a time range up to 100 s60.


EPR spectroscopy

X-band EPR measurements were performed with an Elexsys E580 (Bruker BiosSpin) equipped with a standard cavity (ST 4102). All measurements were performed at low temperature that was achieved with the use of a helium flow cryostat and an ICT-4 temperature controller (Oxford Instruments, UK). The time-resolved EPR (TREPR) measurements were performed with the use of the ADF fast digitizer board (2 MHz fixed sampling rate). Signal acquisition was synchronized with 5 Hz laser flashes using a LC880 TTL pulse generator (100 MHz internal clock) (LabSmith, Livermore, California). An accessory transformer was used to amplify the TTL pulses to the 5 V amplitude required for triggering the laser lamp and Q-switch. Analysis of the EPR spectra was carried out with the Bruker Xepr 2.1 software.

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Acknowledgements

This work was supported by the Swedish Research Council (VR) and the Swedish Energy agency.

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  1. Molecular Biomimetics, Department of Chemistry — Ångström, Box 523, Uppsala University, SE 751 20, Uppsala, Sweden

    • Andrea Pavlou
    • , Julien Jacques
    • , Nigar Ahmadova
    • , Fikret Mamedov
    •  & Stenbjörn Styring

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Contributions

S.S. and F.M. conceived the experiments, A.P., J.J. and N.A. conducted the experiments, A.P. and J.J. analysed the results. A.P., S.S., and F.M. wrote the manuscript.

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The authors declare no competing interests.

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Correspondence to Stenbjörn Styring.

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