Dibutyl phthalate (DBP) is well known as a high-priority pollutant. This study explored the impacts of DBP on the metabolic pathways of microbes in black soils in the short term (20 days). The results showed that the microbial communities were changed in black soils with DBP. In nitrogen cycling, the abundances of the genes were elevated by DBP. DBP contamination facilitated 3′-phosphoadenosine-5′-phosphosulfate (PAPS) formation, and the gene flux of sulfate metabolism was increased. The total abundances of ABC transporters and the gene abundances of the monosaccharide-transporting ATPases MalK and MsmK were increased by DBP. The total abundance of two-component system (TCS) genes and the gene abundances of malate dehydrogenase, histidine kinase and citryl-CoA lyase were increased after DBP contamination. The total abundance of phosphotransferase system (PTS) genes and the gene abundances of phosphotransferase, Crr and BglF were raised by DBP. The increased gene abundances of ABC transporters, TCS and PTS could be the reasons for the acceleration of nitrogen, carbon and sulfate metabolism. The degrading-genes of DBP were increased markedly in soil exposed to DBP. In summary, DBP contamination altered the microbial community and enhanced the gene abundances of the carbon, nitrogen and sulfur metabolism in black soils in the short term.
The grain output from the black soil region accounts for 30% of the national staple food production in China1. Plastic films (for mulching cultivation) have been widely used in farm production of the region. Dibutyl phthalate (DBP) bound to the plastics relatively weakly via hydrogen bond or Van der Waals force, and not combine into PVC polymer chain2. It can be easily released into earth surface and groundwater, and is easy to accumulate3. DBP was detected in soils of all seasons, and the highest DBP concentration was determined in summer4. DBP is ubiquitous in soils5. The residual DBP levels reached 14.6 mg kg−1 in black soils and 29.37 mg kg−1 in fluvo-aquic soils5, and exceeded the recommended values in the soil cleanup guidelines (0.08 mg kg−1) adopted by the US Environmental Protection Agency (US EPA)6,7. DBP can lead to biological health problems, including developmental and reproductive toxicity8. DBP has been listed as one of priority pollutants by both the China National Environmental Monitoring Centre and the United States Environmental Protection Agency9 because of its mutagenicity, teratogenicity, and carcinogenicity10. Therefore, it is very important to understand the damaging effects of DBP on the soil ecosystem and soil health.
It is estimated that 1 g of black soil contains one billion microorganisms11. Soil microorganisms are considered as the key components for soil energy flow7,12 and are believed to be the major driving force of ecosystem functions13,14. Soil microorganisms are a significant part of the earth’s biodiversity and play key roles in carbon metabolism, nitrogen cycling and the overall functioning of an ecosystem15,16. Moreover, soil microorganisms play important roles in soil structure and development17. Changes in the microbial community structure not only alter the soil environment but also affect plant growth and soil fertility18. In the current studies, we applied metagenomics analysis and real-time fluorescent quantitative PCR (qPCR) to explore the impacts of DBP on the microbial ecology of black soils.
DBP concentration in soils
Figure 1 showed DBP concentration in black soils incubated for 0 d and 20 days. This result found that DBP concentration was consistent with the concentration of addition in experiment for 0 d (Fig. 1). Compare with 0 d, DBP concentration reduced in all samples of DBP treatment (DBP1, DBP2, DBP3 and DBP4) for 20 days. These results indicated that the residual rate of DBP was more than 50% at 20 days in black soils.
Microbial community structure
The taxonomic profiles of the metagenome analysis, which contained domain, kingdom, phylum, class, order, family, genus and species, were constructed. With increasing DBP concentration, the percentages of Eukaryota were decreased from 2% to 0.9%, and the percentage of total bacteria was increased from 81% to 92%. We focused on the genus level to explain the changes in the microbial community. Based on the metagenome analysis, the results were shown in Fig. 2. The relative populations of the genera Arthrobacter and Nocardioides were increased along with the increase of DBP concentration. The relative abundances of Arthrobacter in CK, DBP1, DBP2, DBP3 and DBP4 were 1.67%, 6.82%, 10.56%, 22.82% and 39.86%, respectively. The relative abundance of Nocardioides in CK, DBP1, DBP2, DBP3 and DBP4 were 1.75%, 1.91%, 2.05%, 3.07% and 6.09%, respectively. On the other hand, the relative levels of some bacteria were decreased by DBP in all samples and showed a negative correlation with DBP concentration; these bacteria included Nitrososphaera, Lactococcus, Streptomyces, Frankia, Solirubrobacter, Conexibacter, Mycobacterium, Rubrobacter, Tetrasphaera, Pseudomonas, Intrasporangium, Actinoplanes, Actinomadura, Enterococcus, Amycolatopsis, Rhodococcus, Pseudonocardia, Acidothermus, Nocardiopsis, and others.
Annotation of metabolic pathways by KEGG analysis
Metabolic pathways, including nitrogen cycling, carbon metabolism (glycolysis, TCA cycle and pentose phosphate), sulfur metabolism and signal regulatory pathways, were identified in DBP-contaminated black soils based on the KEGG database. The original data are shown in Supplementary Tables 1 and 2.
As shown in Fig. 3, DBP contamination changed the flux of nitrogen cycling. The total abundance of nitrogen cycling genes rose significantly in response to the increase of DBP concentration (F = 25.71, P < 0.01) (Fig. 3A and Supplementary Table 7). Nitrite reductase (NirBD) (F = 234.76, P < 0.01), nitrate reductase (NarGHIJ, NasAB and NxrAB) (F = 136.06, P < 0.01), ferredoxin-nitrite reductase (NirA) (F = 188.95, P < 0.01) and nitric oxide reductase (NorBC) genes (F = 43.89, P < 0.01) are the key enzyme genes in nitrogen cycling, and their copy numbers were significantly increased after DBP contamination (Fig. 3B,C and Supplementary Table 7). The copy numbers of related enzyme genes in nitrogen cycling, such as the formamidase (F = 32.49, P < 0.01), nitrilase (F = 7.64, P < 0.05) and nitronate monooxygenase (F = 177.61, P < 0.01) genes, were increased significantly by DBP contamination (Fig. 3C and Supplementary Table 7).
According to KEGG annotation and comparison of metagenome sequences, the flux of the carbon metabolic pathways, which included the glycolysis, TCA cycle and pentose phosphate pathways, were changed by DBP contamination (Fig. 4). As the DBP concentration increased, the total abundance of the glycolysis pathway genes was significantly enhanced in all samples of DBP treatments (F = 27.15, P < 0.01) (Fig. 4A and Supplementary Table 8). The gene abundances of the rate-limiting enzymes of glycolysis, which included pyruvate kinase (F = 109.34, P < 0.01), glucokinase (F = 11.83, P < 0.01) and phosphofructokinase (F = 17.16, P < 0.01), were also significantly promoted in sample of DBP treatments, including DBP1, DBP2, DBP3 and DBP4 (Fig. 4B and Supplementary Table 8). The gene abundances of enzymes related to glycolysis, which included phosphoglucomutase (F = 136.77, P < 0.01), L-lactate dehydrogenase (F = 71.74, P < 0.01), phosphoenolpyruvate carboxykinase (F = 242.13, P < 0.01), pyruvate dehydrogenase (F = 34.25, P < 0.01), acetate-CoA ligase (F = 49.49, P < 0.01), alcohol dehydrogenase (F = 13.41, P < 0.01), aldehyde dehydrogenase (F = 29.50, P < 0.01), dihydrolipoyl dehydrogenase (F = 46.40, P < 0.01), phosphoglycerate mutase (F = 12.50, P < 0.01) and dihydrolipoyllysine-residue acetyltransferase (F = 94.05, P < 0.01), were increased in all samples of DBP treatments (Fig. 4C–E and Supplementary Table 8). The gene abundance of polyphosphate-glucose phosphotransferase (F = 85.18, P < 0.01) was significantly increased in DBP of high concentration (DBP3 and DBP4) (Fig. 4C and Supplementary Table 8). In the TCA cycle, both the total gene abundance (F = 21.68, P < 0.01) and the gene abundance of the rate-limiting enzymes (including isocitrate dehydrogenase (F = 54.68, P < 0.01) and oxoglutarate dehydrogenase (F = 41.37, P < 0.01)) were raised in all samples of DBP treatments (Fig. 5A,B and Supplementary Table 9). The gene abundance of citrate synthase (F = 21.59, P < 0.01) had significance in in DBP of high concentration (DBP3 and DBP4) (Fig. 5B and Supplementary Table 9). The gene abundances of pyruvate carboxylase (F = 187.36, P < 0.01), dihydrolipoyllysine-residue succinyltransferase (F = 33.32, P < 0.01), fumarate hydratase (F = 8.34, P < 0.05), succinate-CoA ligase (F = 9.85, P < 0.01) and aconitate hydratase (F = 18.10, P < 0.01) were also increased (Fig. 5C and Supplementary Table 9). The gene abundance of oxoglutarate synthase (F = 11.89, P < 0.01) was significantly increased in DBP3 (Fig. 5C and Supplementary Table 9). The total gene abundance of the pentose phosphate pathway was positively correlated with DBP concentration (F = 25.65, P < 0.01) (Fig. 6A and Supplementary Table 10). The gene abundances of phosphogluconate dehydrogenase (F = 68.29, P < 0.01) and glucose-6-phosphate dehydrogenase (F = 21.90, P < 0.01), rate-limiting enzymes in the pentose phosphate pathway, were also augmented by DBP contamination in all samples of DBP treatments (Fig. 6B and Supplementary Table 10). The gene abundances of phosphoglucomutase (F = 190.05, P < 0.01), 2-dehydro-3-deoxygluconokinase (F = 97.44, P < 0.01), 6-phosphogluconolactonase (F = 25.85, P < 0.01), 6-phosphofructokinase (F = 19.09, P < 0.01), glucose-6-phosphate isomerase (F = 15.84, P < 0.01) and transketolase (F = 24.27, P < 0.01) were increased by DBP contamination in all samples of DBP treatments (Fig. 6C and Supplementary Table 10). The gene abundance of ribose-phosphate diphosphokinase was significantly enlarged in DBP of high concentration (DBP3 and DBP4) (F = 16.43, P < 0.01) (Fig. 6C and Supplementary Table 10).
The total abundance of sulfur metabolism genes was higher in the contaminated samples than in CK (F = 21.69, P < 0.01) (Fig. 7A and Supplementary Table 11). The gene abundance of sulfate adenylyl-transferase (F = 22.91, P < 0.01), which included Sat, CysND and CysC, was promoted to various extents with increasing DBP concentration (Fig. 7B). The gene abundances of sulfite reductase (F = 18.78, P < 0.01) was significantly enlarged in DBP1 and DBP3, but it was decreased in DBP2 and DBP4 (Fig. 7B and Supplementary Table 11). The gene abundance of sulfate reductase (F = 3.83, P < 0.05) was enhanced in all samples of DBP treatments (Fig. 7B and Supplementary Table 11).
Signal regulatory pathways
The total gene abundances of the ABC transporters (F = 27.45, P < 0.01) and two-component system (TCS) (F = 25.81, P < 0.01) were promoted in all samples of DBP treatments (Fig. 8A and Supplementary Table 12). The total gene abundances of the phosphotransferase system (PTS) were increased in DBP3 and DBP4 (F = 44.73, P < 0.01) (Fig. 8B and Supplementary Table 12). The gene abundances of the monosaccharide-transporting ATPases (F = 31.22, P < 0.01), MalK and MsmK (F = 17.95, P < 0.01) of the ABC transporters were increased under DBP contamination (Fig. 8C and D and Supplementary Table 12). In the TCS, DBP contamination brought about an increase in the gene abundances of malate dehydrogenase (F = 23.12, P < 0.01), histidine kinase (F = 18.06, P < 0.01) and citryl-CoA lyase (F = 75.29, P < 0.01) (Fig. 8C,D and Supplementary Table 12). The gene abundances of the phosphotransferases (F = 116.26, P < 0.01), Crr and BglF (F = 67.57, P < 0.01) in the PTS were significantly increased by DBP and were positively correlated with DBP concentration (Fig. 8C and Supplementary Table 12).
Nonmetric multidimensional scaling (NMDS) analysis
To explain the overall differences among the CK and DBP contamination samples, NMDS analysis was performed at the levels of genes, taxonomy and metabolic pathways. The results indicated that DBP contamination altered the distribution of genes, taxons and metabolic genes (Fig. 9). Furthermore, the difference became larger with increasing DBP concentrations.
DBP degradation genes
The main DBP degradation genes are pcmA, pehA, phtAb, phtB and phtC. The copy numbers of pcmA, pehA, phtAb, phtB and phtC were significantly increased under DBP contamination (Fig. 10). When the concentration of DBP increased, the copy numbers increased as well, and the range of increase varied. The lowest degree of change was that of pcmA, which increased from 3.06 × 104 to 1.05 × 105. The greatest change was that of phtB, which increased from 1.55 × 103 to 4.39 × 105. Additionally, the copy numbers of pehA and phtAb genes were elevated along with increasing DBP concentration, increasing from 3.29 × 103 to 2.42 × 105 and from 3.13 × 104 to 1.62 × 105, respectively. After DBP contamination, the copy number of phtC was higher compared with CK, increasing from 94.49 to 5.35 × 104. The results were consistent with the change of DBP concentration in black soil (Fig. 1).
Using metagenomic analysis, this study found that the microbial community structure was changed in black soil under DBP contamination in the short term. The abundances of some genera, such as Arthrobacter and Nocardioides, were significantly enriched in response to DBP treatment. Previous studies showed that Arthrobacter and Nocardioides could degrade organic contamination19,20. This phenomenon could be further demonstrated by comparing the copy numbers of DBP degradation genes (Fig. 10). However, the growth for some genera that are indispensable for nutrient cycling21,22,23 were inhibited by DBP, including Nitrososphaera, Streptomyces, Frankia, Conexibacter, Pseudonocardia, Acidothermus, Nocardiopsis, Lactococcus, Mycobacterium, Pseudomonas and Tetrasphaera. Nitrososphaera, Frankia and Conexibacter. Streptomyces24,25, Conexibacter26, Pseudonocardia27, Acidothermus28, Nocardiopsis29, Lactococcus30, Mycobacterium31,32, Pseudomonas33 and Tetrasphaera34 are beneficial to soil health and plant growth. Amycolatopsis and Intrasporangium were also decreased even though they could remediate heavy metal pollution35,36. The changes in the microbial community indicated that DBP contamination disturbed the soil ecosystem in the short term.
Nitrogen cycling, carbon metabolism and sulfur metabolism are the basis of nutrient cycling in soil ecosystems37,38,39. The turnover of the nitrogen cycle, the glycolysis pathway, the TCA cycle, the pentose phosphate pathway and sulfur metabolism were accelerated significantly when soil microorganisms were exposed to DBP contamination. In nitrogen cycling, the genes NarGHIJ, NasAB, NxrAB, NirBD, and NorBC are the key genes to the dissimilatory nitrate reduction, assimilatory nitrate reduction, denitrification and nitrification, and their abundances were elevated due to DBP contamination. Meanwhile, the gene abundances of NorBC and NarGHIJ were increased. Because NorBC and NarGHIJ catalyze the generation of nitrous oxide (N2O) and nitrite40, nitrite accumulation and N2O emission could be increased. In this study, it was found that the turnover of the carbon metabolic pathways was accelerated after DBP treatment such that more carbons in the substrate were converted to CO2, which meant that more carbon could be consumed in the soils. It was also found that the gene abundance of sulfate adenylyltransferase was increased in the soil microorganisms. The phenomenon implied that DBP contamination facilitated 3′-phosphoadenosine-5′- phosphosulfate (PAPS) formation. This result could indicate that the flux of sulfate metabolism was increased and that more sulfate turned toward the direction of oxidative metabolism after DBP contamination39,41,42. For all of these results, the metabolic processes of soil microorganisms were accelerated including nitrogen cycling, carbon metabolism and sulfur metabolism, which could affect the nutrient transformation and the quality of black soils in the short term.
ABC transporters are found in all domains of organisms and transfer a remarkably wide range of substrates into and out of living cells43,44. ABC transporters are useful for transporting substrates including sugars, amino acids, peptides, ions and other molecules as well as for absorbing nutrients into cells45,46. Increases in the total abundance of ABC transporters and the relative gene abundances of the monosaccharide-transporting ATPases MalK and MsmK could be the reason for the accelerated metabolism of nitrogen, carbon and sulfate, which may accelerate the depletion of nutrients in black soils. In prokaryotes, TCS is a sophisticated signal transduction strategy and senses any alteration in the environment47. It regulates downstream gene expression, thus orchestrating several cellular responses, and participates in biochemical processes, acting as an energy carrier48. The total abundance of TCS genes and the abundances of the malate dehydrogenase, histidine kinase and citryl-CoA lyase genes were increased after DBP contamination, suggesting that it could lead to accelerated metabolism in black soils, including nitrogen, carbon and sulfate metabolism. PTS is the key signal transduction pathway involved in the regulation of central carbon and nitrogen metabolism in bacteria49,50. The total abundance of PTS genes and the gene abundances of phosphotransferase, Crr and BglF were raised by DBP. These manifestations indicated that the increase of PTS was one of the causes of the acceleration of nitrogen and carbon metabolism. Hence, these changes of ABC transporters, TCS and PTS brought about metabolic changes in black soils in the short term.
Based on the results of metagenome analysis and qPCR analysis in the short term (20 days), we propose that DBP contamination altered the structure of the microbial community; improved the gene abundances of nitrogen cycling, glycolysis, the TCA cycle, the pentose phosphate pathway and sulfate metabolism; and increased the abundance of DBP degradation genes. The increased abundances of signal regulatory pathways for ABC transporters, TCS and PTS could be the reason for the changes of gene abundances in carbon, nitrogen and sulfur metabolism. However, it remains to be seen whether structure and function are impacted over the long-term.
Materials and Methods
Sample collection and treatment
DBP (chromatographically pure grade) was purchased from the Information Center for Standard Samples (Beijing, China). Acetone (chromatographically pure grade) was acquired from Traditional Chinese Medicine (Beijing, China). DBP was dissolved in acetone at a ratio of 1 to 9 (m/m) and then stored in the dark at 4 °C.
The black soil samples were collected randomly from various areas of Keshan County of Heilongjiang Province in China, which had no history of DBP contamination as of August 5, 2015. All soils were under the same crop, and then excavating soils 200 kg within 20 cm of the black soil surface after removing the top surface layer in different locations. The physicochemical properties of the black soils are shown in Table 1. Soils were sieved by 0.3 mm mesh. After blending and sieving, the soils were divided into 15 samples of 1000 g each. Afterwards, the soil was moistened up to 30% and preincubated at 25 °C for 7 days. Varied amounts of DBP were added to the soil samples and mixed well, as follows: Control (CK), 0 mg DBP kg−1 soil; DBP sample 1 (DBP1), 5 mg DBP kg−1 soil; DBP sample 2 (DBP2), 10 mg DBP kg−1 soil; DBP sample 3 (DBP3), 20 mg DBP kg−1 soil; and DBP sample 4 (DBP4), 40 mg DBP kg−1 soil. The measured concentrations of DBP from the different treatments are shown in the Supplementary Figure 1. Each concentration stress was repeated 3 times. All the samples were exposed to the air for 3 h until the acetone was evaporated completely. The soil samples were placed in porcelain pots and incubated in the dark at 25 °C for 20 days. During the period of experiment, all samples were maintained at 30% moisture. DBP concentrations were measured in black soils incubated for 0 d and 20 days in the dark at 25 °C and 70% air humidity in constant temperature and humidity incubator.
DMP concentration in soil
First, DBP of different samples were extracted using rotary evaporators. Then, DBP concentrations were measured by liquid chromatography for 0 d and 20 days. Parameters of liquid chromatography: sample size = 1 μl; Chromatographic column was 5 μl Eclipse XDB-C18; The chromatographic conditions: the temperature of the column = 25 °C, flow phase: methanol and water, volume ratio = 90:10; Detection wavelength = 228 nm.
DNA extraction, metagenomic sequencing and paired-end (PE) library construction
Ten grams of soil were taken out from each pot. Then, the total DNA was extracted from the samples and used for the metagenome sequencing and further analyses51. E.Z.N.A.® Soil DNA Kit was used to extract the soil DNA (Supplementary Table 3). DNA concentration, purity, and integrity were detected by NanoDrop2000, TBS-380 and agarose gel electrophoresis, respectively. 1 μg DNA was used to build library. Briefly, the DNAs were sheared using an M220 Focused-ultrasonicator™ (Covaris Inc., Woburn, MA, USA) and the parameters of instrument were set according to Supplementary Table 4. The PE library was composed of ~300 bp DNA fragments. The TruSeq® DNA Sample Prep Kit was used to prepare DNA library based on the manufacturer’s protocol (www.illumina.com). Primer hybridization sites were included in dual-index adapters and ligated to the blunt-end fragments. To improve quality of the template DNA, paired-end sequencing (2 × 151 bp) was conducted on an Illumina Genome Analyzer (Illumina Inc., USA) by Majorbio Bio-Pharm Technology Co., Ltd. (Shanghai, China). All the raw metagenomic datasets have been deposited into the NCBI Sequence Read Achieve (accession numbers: SRP094732, SRP096214).
Sequence quality control, assembly and gene prediction
To improve the quality and reliability of subsequent analysis, the raw data were processed using the following steps:
SeqPrep (https://github.com/jstjohn/SeqPrep) was used to remove the reads that contained N bases in the sequence and strip the 3′-adaptors and 5′-adaptors.
To retain high-quality pair-end reads and single-end reads, the reads of sequence length <50 bp and quality score <20 were excised with Sickle (https://github.com/najoshi/sickle). Clean reads were assembled with SOAPdenovo (http://soap.genomics.org.cn,Version 1.06) with a default k-mer length (39–47), and the de Bruijn graphs were used to build contigs. For the optimal assembly, scaffolds over 500 bp were divided into new contigs without gaps. The open reading frames (ORFs) of the contigs from the assembled results were predicted using MetaGene Annotator (http://metagene.cb.k.u-tokyo.ac.jp/).
Taxonomy and functional annotation
The predicted ORFs of ≥100 bp were selected and translated to amino acid sequences by the NCBI translation table (http://www.ncbi.nlm.nih.gov/Taxonomy/taxonomyhome.html/index.cgi?chapter = tgencodes#SG1), and the sequences were subsequently annotated by searching BLASTP (Version 2.2.28+, http://blast.ncbi.nlm.nih.gov/Blast.cgi) against the NCBI NR database containing SwissProt, PRF (Protein Research Foundation), PIR (Protein Information Resource), PDB (Protein Data Bank), and protein information resources from corresponding coding sequences (CDS) on RefSeqG and GenBank. The BLAST parameters were set as follows: E-value cutoff = 1*10−5, num_alignments = 250, and num_descriptions = 500.
The taxonomy annotation was obtained based on the taxonomic information database corresponding to the NCBI NR library. The species abundance was calculated using the total gene abundance across all species, and the abundance profile was built based on taxonomic levels, which were the domain, kingdom, phylum, class, order, family, genus and species.
The KEGG database (Kyoto Encyclopedia of Genes and Genomes, http://www.genome.jp/kegg/) is a huge library for systematic analysis of gene function, combining genome and function information. It provides sequence information on the genes and proteins identified in genome projects. Further, the database includes various pathways, such as metabolic, biosynthetic, membrane transport, signaling, cell cycle and disease pathways. In addition, it collects records of all kinds of enzymes and other molecules as well as enzymatic reactions and related information. The KEGG pathway annotation was done using KOBAS 2.0 (KEGG Orthology Based Annotation System, http://kobas.cbi.pku.edu.cn/home.do) according to the alignment results between the gene set sequence and the KEGG database using BLAST (BLAST Version 2.2.28+, http://blast.ncbi.nlm.nih.gov/Blast.cgi). The amassed read data for each sample are presented in Supplementary Table 5.
Nonmetric multidimensional scaling (NMDS)
Nonmetric multidimensional scaling (NMDS) is a monotonic function that evaluates the similarities or differences of different samples based on distance. In this study, NMDS represented the overall difference between control and DBP (varied concentration) contamination samples in taxonomy, genes and KEGG pathways. NMDS was generated by R language release package (MASS package).
Real-time fluorescent quantitative PCR (qPCR)
qPCR was performed to detect the genes associated with the degradation of DBP, including phthalate ester hydrolase (pehA), 3,4-dihydrophthalate dehydrogenase (phtB), phthalate dioxygenase small subunit (phtAb), protocatechuate 4,5-dioxygenase (pcmA) and 3,4-dihydrophthalate decarboxylase (phtC)52. The primer information for these genes is shown in Table 2. PCR was conducted in 20-μL reactions containing 10 μL of 2 × SYBR green qPCR master mix (premix of dNTPs, Taq DNA polymerase, PCR buffers and SYBR green), 0.4 μL of primer F and 0.4 μL of primer R of each gene-specific primer pair, 7.2 μL of ddH2O and 2 μL of template (DNA) based on the manufacturer’s instructions (LightCycler480, Roche). DNA concentration was shown in Supplementary Table 6. The qPCR thermal cycling steps are shown in Table 3. The melting curves for the genes were obtained during the detection, representing the specificity of the amplification. A linear relationship was shown based on standard curves ranging from 103 to 1010 copies. The amplification efficiencies ranged from 71.2% to 107.8%, and R2 ranged from 0.9901 to 0.9984 (Supplementary Figure 2). Analyses of variance (ANOVA) and the least significant ranges test (Duncan’s method) were performed with SPSS 22.0 to test the significance (p < 0.05) of the differences among the treatments.
Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
We gratefully acknowledge financial supports of Natural Science Foundation of China (31670375), Graduate Innovation Project of Qiqihar University (YJSCX2016-ZD16) and Foundation of Key Laboratory of Urban Agriculture in South China, Ministry of Agriculture (003). We also thank Dr. Lauren Hale from the Department of Microbiology and Plant Biology, University of Oklahoma, for her useful comments and language editing which have greatly improved the manuscript.
Electronic supplementary material
About this article
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.