Abstract
At broad scales in the Amazon, it is often hypothesized that species distributions are limited by geographical barriers, such as large rivers (river-barrier hypothesis). This hypothesis has been used to explain the spatial-distribution limits of species and to indicate endemism areas for several phylogenetic lineages. We tested the ability of the river-barrier hypothesis to explain patterns of species diversity and spatial-distribution limits for 1952 easily-detected species in 14 taxonomic groups that occur around the Madeira River, and our results indicate that the hypothesis that the Madeira River is the border between endemism areas and explains much of the diversity found in the region is inappropriate for >99% of species. This indicates that alternative hypotheses should be proposed to explain the limits of distributions of species around the Madeira River, as well as a revision of the criteria that are used to determine species-endemism areas.
Similar content being viewed by others
Introduction
Presence or absence of individuals of a species in the Amazon can be attributed to multiple factors. At local scales, habitat characteristics have been identified as the main determinants of the distribution of various of plants1,2,3,4, lizards5, anurans6,7, snakes8, ants9, mammals10,11,12, termites13 and birds14,15. However, at broader scales, it is often hypothesized that species distributions are mainly related to dispersal limitation caused by geographical barriers, such as large rivers16,17. This explanation is commonly referred to as the “river-barrier hypothesis”.
Wallace18 was one of the first to hypothesize that the distributions of Amazonian species could be limited by large Amazonian rivers, such as the Negro, Amazon and Madeira Rivers. According to the modern interpretation of this hypothesis, large rivers are expected to subdivide a population to the point of preventing gene flow between individuals in different areas and to promote genetic divergence between them, increasing the opportunity for allopatric speciation19,20. If this hypothesis is correct, it is expected that (i) sister species or lineages will be on opposite river banks21,22,23, (ii) the similarity in species composition will be greater in localities on the same bank (adjacent sites) than sites on opposite banks separated by the same distance24,25,26,27 and (iii) the boundaries of species distributions will coincide with large rivers21,22,23,24,25,26,27,28.
The river-barrier hypothesis has been used to explain the spatial-distribution limits of species and to indicate possible endemism areas29,30 for several phylogenetic lineages in the several taxa in the Amazon (e.g. primates23,24, lizards17,28, anurans16,17,25, butterflies21, birds22,26,27,31). The hypothesized endemism areas delimited by rivers have been used as surrogates in conservation planning30. However, this hypothesis is not always accepted and the role of rivers as the limits to endemism areas has been questioned for many taxa13,17,26,27,31,32,33,34,35,36,37,38,39,40,41. For example, the effects of the Tapajós River (for amphibians and squamates17) and the Amazon River (for birds26) as barriers depend on the life-history characteristics of the species. Dambrós et al.13 showed that sites separated by large geographic distances had distinct termite-species composition and most of the broad-scale variation in species composition could be explained either by spatial predictors or differences in environmental conditions between regions, and not by large rivers, such as the Madeira, Negro, Branco and Amazon.
In the majority of the studies that accepted the river-barrier hypothesis, the conclusions were based on studies with few species20,23 and on the assumed absence of species on one bank16,17,24. In addition, rivers vary in discharge and width, and these two factors have been considered important in determining when large rivers function as geographic barriers to species dispersal24,36,42. Therefore, the acceptance or rejection of the hypothesis may depend mainly on the species and river investigated. These two factors together make it difficult to generalize the importance of large rivers as effective geographical barriers to the distribution of Amazonian species and as a possible hypothesis to explain the species diversity found in the region.
In this study, we estimated the proportion of species in different taxonomic groups [Hymenoptera (Apidae), Hymenoptera (Formicidae), Coleoptera, Lepidoptera, Isoptera, Orthoptera, Snakes, Lizards (excluding snakes), Anura, Chiroptera, Primates, Small mammals (Didelphimorphia, Rodentia), Large mammals (Rodentia, Pilosa, Ungulados, Carnivora, Artiodactyla, Cingulata) and Birds] that have their distributions limited by a river (the generic hypothesis of the river as a barrier) and the number of species for which there is evidence (sister species on opposite banks of the river) that this river functioned as a vicariance barrier causing speciation (the hypothesis of existence of endemism areas based on large rivers). We used only species for which false absences are unlikely to explain the appearance of the river as a barrier. We conducted the study on the Madeira River, which has been postulated as a barrier to dispersal for species of various taxa16,18,22,38,43,44,45,46,47 and the border between two endemism areas29,30, and we studied an area in the mid reaches where many studies have indicated that it is an effective biogeographic barrier. Our results indicate that the hypothesis that the Madeira River is the border that separates two endemism areas (Inambari and Rondonia) and that the river-barrier hypothesis explains much of the diversity found in the region is inappropriate for most species, and we suggest that alternative hypotheses should be proposed to explain the limits of distributions of most species found in the region, as well as a revision of the criteria that are used to determine species-endemism areas.
Results
Generic hypothesis of large rivers as barriers
The hypothesis that the distribution of species around the Madeira River is mainly related to dispersal limitation caused by river barriers, was rejected for most species studied (Fig. 1, Supplementary Table S1). Of the 1952 species with detection probabilities sufficiently high that false absences are improbable, only 0.10% (Primates: Saguinus labiatus labiatus and Aves:Lepidothrix coronata) had their distributions limited by the river (Supplementary Figs S1 and S2).
Because the proportion of species limited to one side of the river depends on our decision as to which species the detection probability was high enough for a valid test, our results might underestimate the number of species limited to one side of the river if the species that are limited by the river are those that are difficult to detect. Therefore, we report the number of species in each taxonomic or functional group that had their distributions limited by the river considering other P expected in Supplementary Table S1, and give their distributions in Supplementary Figs S1 and S2 (only for species with detection probability ≥ 0.40). The number of species apparently separated by the river was low in all cases, except when we made absolutely no correction for probable false absences (Supplementary Table S1).
Hypothesis of the existence of endemism areas based on large rivers
Evidence that the Madeira River works as a vicariance barrier causing speciation (presumption of the endemism-areas hypothesis) was not found for 713 (99.45%) of the species investigated for which we could obtain data to erect robust phylogenetic hypotheses (Supplementary Figs S3–S7). We found evidence suggesting that the river had functioned as a vicariance barrier only for 4 (0.55%) of the species [Primates: Callicebus brunneus e Callicebus dubius (Fig. 2) and Aves: Psophia viridis e Psophia leucoptera (Fig. 3)].
Discussion
The hypothesis of the Madeira River as the limit of distribution was not supported for most species, so our results are not concordant with the river-barrier hypothesis explaining the origin20,22 or spatial-distribution limits of species16,17, nor of the existence of endemism areas29,30, for most of the species that occur around the Madeira River. Even if the hypothesis is correct that the effectiveness of the river as a barrier depends on the characteristics of life histories of the species for a small proportion of some taxa17,26, this would explain only a very small part of the biological diversity of the Amazon40,48,49.
In most studies that accepted the hypotheses about the effects of rivers16,17,24, the apparent absence of a species on the opposite bank to that sampled was used to conclude that a large river was a geographical barrier. However, any species-sampling technique has some bias and the absence of a species in a certain location might indicate that the species was simply not detected50.
For example, Dias-Terceiro et al.16 found that the distribution of Ameerega trivittata (Anura:Dendrobatidae) was restricted to the left bank of the Madeira River (accepting the generic hypothesis of large rivers). This species was recorded on both banks in the Madeira River in our study and also on the left bank of the Tapajós River in the study by Moraes et al.17. The Tapajós River is located adjacent to the right bank of the Madeira River, and the presence of a species on the left bank of the Tapajós River implies the presence of A. trivittata on the right bank of the Madeira River. Fecchio et al.47 concluded that the composition of parasites in birds was dependent on endemism areas in the Amazon, but some of the host species that supported this conclusion occurred in our samples independent of the endemism area. It is possible that the conclusion of these authors was biased by the false absence of the host in one of the areas of endemism. This possible bias in conclusions has been observed for other species in Cracraft29, a reference that has been widely used to support and justify studies that determine endemism areas in the Amazon, based only on the apparent absence of a species on the opposite bank of a large river. It is possible that these are not the only cases of doubtful results in the literature, since this type of potential error was detected many times in our analyses. In approximately 40% of the species, the detectability analysis indicated that sampling was inadequate to draw a conclusion. It could be that only hard-to-detect species are affected by rivers, but this seems unlikely since the river-barrier hypotheses were raised based on easily-detected species.
It is unquestionable that large rivers are the distribution limits of some Amazonian species, but the large number of exceptions indicates that the indication of the Madeira River as a border between endemism areas may be inappropriate for most species. It is important to emphasize that rivers can function as species limits without necessarily indicating that they represent barriers that caused vicariance speciation51, an assumption of the existence of endemism areas based on large rivers. Alternatively, sympatric speciation via sexual selection52,53, environmental differences54,55,56 or ecological interactions57,58; combined with dispersal limitation51,59 and competition60 could produce the same patterns of allopatric distribution observed in Figs 2 and 3, and also in Ribas et al.22, Fernandes et al.20, Boubli et al.23 and are likely more important mechanisms for generating and maintaining Amazonian biodiversity than rivers. However, these alternative hypotheses are often ignored in studies that accept the hypothesis of large rivers as the cause of speciation. Moreover, most of the conclusions relating to the river-barrier hypotheses assume that the geographical distribution of a species does not change over time, but there is evidence that many distributions in the past were different from current distributions61,62,63,64,65.
The lack of evidence found to support the river-barrier hypotheses (generic hypothesis of large rivers as barriers, and the hypothesis of centers of endemism based on large rivers) in a stretch of river commonly postulated as the border between endemism areas16,18,29,30,38,44,45,46,47, suggests that the hypothesis of existence of endemism areas based only on the distributions of a few species and very large rivers, should be reevaluated for the majority of species. With the reevaluation of these limits, the need for new hypotheses will arise to explain the Madeira River’s role in the origin and distribution of Amazonian biodiversity. More importantly, in the absence of information on the distributions of most species, the proposed endemism areas are being used as surrogates in conservation planning30. Substitutes should only be used when there is strong evidence of the relationship between the majority of targets and the proposed substitute66. In the case of centers of endemism, this evidence is not available for most Amazonian rivers, and specifically for the Madeira River, the evidence that it is a border between endemism areas applies to a very small proportion of biodiversity.
Our results are for only one area and there are taxonomic issues relating to species boundaries that need to be worked out for many taxa. Most of the species we studied are recognized on morphological criteria and with the application of molecular methods more species could be discovered that have the Madeira River as a limit to their distributions. Nevertheless, our results indicate that the roles of large rivers in promoting biological diversity and the use of postulated endemism areas as convenient surrogates for conservation planning in the Amazon still need to be tested for the particular taxonomic group and conservation question being addressed.
Methods
Study area
We undertook the study along the Madeira River (Fig. 4), one of the main tributaries of the Amazon River. The section of the river investigated is in the region where the river has a width of approximately 1.6 km, which has been considered a strong barrier in many previous studies16,18,22,38,44,45,46,47 and the border between endemism areas29,30.
Data source
To estimate the proportion of species whose distributions are effectively delimited by the river, we took advantage of an intensive study of the fauna associated with the implantation of a hydroelectric dam on the Madeira River. Sampling was carried out on both banks of the river, following the RAPELD protocol67 (Supplementary Fig. S8). Some species may be limited by rivers but not occur on the immediate banks due to habitat-type (e.g. flooded area) avoidance. However, the field infrastructure comprised two parallel 5-km trails (Supplementary Fig. S8) and also sampled non-flooded area. The number of samples per bank and taxonomic groups surveyed are listed in Supplementary Table S2. In this study, we investigated only the distributions of animal species, since none of the evidence used to propose the river–barrier hypothesis was based on information about plants or microorganisms.
Data analysis
Generic hypothesis of large rivers as barriers
It was not possible to test the hypothesis for all the species of the region, because little is known about the distributions of many species, and many Amazonian species have not yet been described. As surveys of each taxonomic or functional group were made by the same researchers, we could include non-described species (hereafter referred to as morphospecies), for those species for which detectability analyses indicated that the absence of records on one bank of the river had little chance of being due to false absences.
In order to obtain an unbiased estimate of the proportion of species in each taxonomic or functional group [Hymenoptera (Apidae), Hymenoptera (Formicidae), Coleoptera, Lepidoptera, Isoptera, Orthoptera, Snakes, Lizards (excluding snakes), Anura, Chiroptera, Primates, Small mammals (Didelphimorphia, Rodentia), Large mammals (Rodentia, Pilosa, Ungulados, Carnivora, Artiodactyla, Cingulata) and Birds] that had their distributions limited by the river, we considered that the river was a potential geographical barrier only when detectability analyses indicated that the expected probability (P expected ) of the species truly being absent from one of the banks (right or left) was P expected ≥ 0.50. This criterion allows us to conclude that the absence of a species on the opposite bank to which it was present is unlikely to be due to false absences caused by failures in the detection of the species. This expected probability was estimated according to the formula:
where: P expected , is the expected probability of the species occurring on the bank opposite to that on which it was recorded; N is the number of samples where the species occurred; NsampleBank is the total number of samples on the bank where the species was present (right or left bank); NsampleOppositeBank, is the total number of samples on the opposite bank to which the species was recorded.
Hypothesis of the existence of centers of endemism based on large rivers
The hypothesis of the existence of endemism areas based on large rivers was tested for 717 species (no false absences taken into account) of vertebrates for which it was possible to obtain phylogenetic information. To indicate if the river worked as a vicariance barrier independent of the taxonomic or functional group, we constructed a phylogenetic hypothesis separately for each group (Figs S3–S7). For small, large and non-flying mammals (72 spp), snakes (66 spp), lizards (35 spp) and frogs (98 spp), the phylogenetic relationships were obtained with the R package “rotl”68, and for birds (446 spp) the information was obtained through the website birdtree.org69,70,71.
To determine the number of sister species or lineages for which the river was an apparent vicariance barrier, we associated each species in the phylogenetic hypotheses (referring to the different taxonomic or functional groups) with their location of occurrence (right or left bank of the river). If sister species or lineages (indicated by the phylogenetic hypothesis) were present on opposite banks (allopatric distribution), this result could be an indication that the river functioned as a vicariance barrier.
Avoiding potential sample biases
Before accepting the generic hypothesis of large rivers as barriers, and the hypothesis of existence of endemism areas based on large rivers, and to minimize the effect of sampling on the results, we checked the distribution of each species that apparently occurred only on one bank based on the data from Santo Antônio with records in the literature and in the websites of the Global Biodiversity Information Facility (http://www.gbif.org), speciesLink (http://www.splink.org.br, Information system that integrates in real time, primary data of scientific collections), Portal da Biodiversidade (https://portaldabiodiversidade.icmbio.gov.br/portal/, this site provides data and information on Brazilian biodiversity generated or received by the Ministry of the Environment and related institutions) and the Smithsonian National Museum of Natural History (https://naturalhistory.si.edu/).
Data Availability
The datasets analyzed during the current study were collected during the environmental-impact studies for the Santo Antônio hydro-electric reservoir and are of open-access through the web site of the Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis (IBAMA) web site. However, due to some inconsistencies in that data base, the data used here were provided by Santo Antônio Energia and were further quality checked. They are available from the corresponding author on request.
References
Tuomisto, H. & Poulsen, A. D. Pteridophyte diversity and species composition in four Amazonian rain forests. J. Veg. Sci. 11, 383–396 (2000).
Emilio, T. et al. Soil physical conditions limit palm and tree basal area in Amazonian forests. Plant Ecol. Divers. 7, 215–229 (2014).
Schietti, J. et al. Vertical distance from drainage drives floristic composition changes in an Amazonian rainforest. Plant Ecol. Divers. 7, 1–13 (2013).
Zuquim, G. et al. Predicting environmental gradients with fern species composition in Brazilian Amazonia. J. Veg. Sci. 25, 1195–1207 (2014).
Garda, A. A. et al. Microhabitat Variation Explains Local-scale Distribution of Terrestrial Amazonian Lizards in Rondônia, Western Brazil. Biotropica 45, 245–252 (2013).
Menin, M., Lima, A. P., Magnusson, W. E. & Waldez, F. Topographic and edaphic effects on the distribution of terrestrially reproducing anurans in Central Amazonia: mesoscale spatial patterns. J. Trop. Ecol. 23, 539–547 (2007).
Menin, M., Waldez, F. & Lima, A. P. Effects of environmental and spatial factors on the distribution of anuran species with aquatic reproduction in central Amazonia. Herpetol. J. 21, 255–261 (2011).
Fraga, R. D., Lima, A. P. & Magnusson, W. E. The width of riparian corridors in central Amazonia. Herpetol. J. 21, 51–57 (2011).
Vasconcelos, H. L., Macedo, A. C. C. & Vilhena, J. Influence of Topography on the Distribution of Ground-Dwelling Ants in an Amazonian Forest. Stud. Neotrop. Fauna Environ. 38, 115–124 (2003).
Bernard, E. Vertical stratification of bat communities in primary forests of Central amazon, Brazil. J. Trop. Ecol. 17, 2001 (2001).
Haugaasen, T. & Peres, C. A. Mammal assemblage structure in Amazonian fooded and unflooded forests. J. Trop. Ecol. 21, 133–145 (2005).
Lambert, T. D., Malcolm, J. R. & Zimmerman, B. L. Amazonian Small Mammal Abundances in Relation To Habitat Structure and Resource Abundance. J. Mammal. 87, 766–776 (2006).
Dambros, C. S., Azevedo, R. A. & Gotelli, N. J. Isolation by distance, not rivers, control the distribution of termite species in the Amazonian rain forest. Ecography (Cop.). 39, 1–9 (2016).
Bueno, A., Bruno, R., Pimentel, T., Sanaiotti, T. & Magnusson, W. E. The width of riparian habitats for understory birds in an Amazonian forest The width of riparian habitats for understory birds in an Amazonian forest. Ecol. Appl. 22, 722–734 (2012).
Cintra, R. & Naka, L. N. Spatial variation in bird community composition in relation to topographic gradient and forest heterogeneity in a central amazonian rainforest. Int. J. Ecol. 2012 (2012).
Dias-terceiro, R., Kaefer, I. L., Fraga, R. & Lima, A. A Matter of Scale: Historical and Environmental Factors Structure Anuran Assemblages from the Upper Madeira River. Biotropica . 47, 259–266 (2015).
Moraes, L., Pavan, D., Barros, M. C. & Ribas, C. Combined influence of riverine barriers and flooding gradient on biogeographical patterns of amphibians and squamates in South-eastern Amazonia. J. Biogeogr. 43, 2113–2124 (2016).
Wallace, A. On the monkeys of the Amazon. Proc. Zool. Soc. London 20, 107–110 (1852).
Wiley, E. O. Vicariance Biogeography. Annu. Rev. Ecol. Syst. 19, 513–542 (1988).
Fernandes, A. M., Wink, M. & Aleixo, A. Phylogeography of the chestnut-tailed antbird (Myrmeciza hemimelaena) clarifies the role of rivers in Amazonian biogeography. J. Biogeogr. 39, 1524–1535 (2012).
Hall, J. P. W. & Harvey, D. J. The Phylogeography of Amazonia Revisited: New Evidence from Riodinid Butterflies. Evolution (N. Y). 56, 1489–1497 (2002).
Ribas, C., Aleixo, A., Nogueira, A. C. R., Miyaki, C. Y. & Cracraft, J. A palaeobiogeographic model for biotic diversification within Amazonia over the past three million years. Proc. R. Soc. B. 279, 681–689 (2011).
Boubli, J. P. et al. Spatial and temporal patterns of diversification on the Amazon: A test of the riverine hypothesis for all diurnal primates of Rio Negro and Rio Branco in Brazil. Mol. Phylogenet. Evol. 82, 400–412 (2015).
Ayres, J. M. & Clutton-Brock, T. H. River Boundaries and Species Range Size in Amazonian Primates. Am. Nat. 140, 531–537 (1992).
Ron, S. R. Biogeographic area relationships of lowland Neotropical rainforest based on raw distributions of vertebrate groups. Biol. J. Linn. Soc. 71, 379–402 (2000).
Hayes, F. E. & Sewlal, J. The Amazon River as a Dispersal Barrier to Passerine Birds: Effects of River Width, Habitat and Taxonomy. J. Biogeogr. 31, 1809–1818 (2004).
Pomara, L. Y., Ruokolainen, K. & Young, K. R. Avian species composition across the Amazon River: The roles of dispersal limitation and environmental heterogeneity. J. Biogeogr. 41, 784–796 (2014).
Avila-Pires, T. C. S. Lizards of Brazilian Amazonia (Reptilia: Squamata). Zoologische Verhandelingen 299, 1–706 (1995).
Cracraſt, J. Historical biogeography and patterns of differentiation within the South American avifauna: areas of endemism. Ornithol. Monogr. 49–84 (1985).
Da Silva, J. M. C., Rylands, A. B. & Da Fonseca, G. A. B. The fate of the Amazonian areas of endemism. Conserv. Biol. 19, 689–694 (2005).
Naka, L. N., Bechtoldt, C. L., Henriques, L. M. P. & Brumfield, R. T. The Role of Physical Barriers in the Location of Avian Suture Zones in the Guiana Shield, Northern Amazonia. Am. Nat. 179, E115–E132 (2012).
Gascon, C. Amphibian Litter Fauna and River Barriers in Flooded and Non-Flooded Amazonian Rain Forest. Biotropica 28, 136–140 (1996).
Gascon, C., Lougheed, S. C. & Bogart, J. P. Genetic and Morphological Variation in Vanzolinius discodactylus: A Test of the River Hypothesis of Speciation. Biotropica 28, 376 (1996).
Gascon, C., Lougheed, S. C. & Bogart, J. P. Patterns of Genetic Population Differentiation in Four Species of Amazonian Frogs: A Test of the Riverine Barrier Hypothesis. Biotropica 30, 104–119 (1998).
Lougheed, S. C., Gascon, C., Jones, D. A., Bogart, J. P. & Boag, P. T. Ridges and rivers: a test of competing hypotheses of Amazonian diversification using a dart-poison frog (Epipedobates femoralis). Proc. R. Soc. Lond. B 266, 1829–1835 (1999).
Gascon, C. et al. Riverine barriers and the geographic distribution of Amazonian species. Proc. Natl. Acad. Sci. 97, 13672–13677 (2000).
Fairley, T. L. et al. Evaluation of the Amazon River delta as a barrier to gene flow for the regional malaria vector, Anopheles aquasalis (Diptera: Culicidae) in northeastern Brazil. J. Med. Entomol. 39, 861–9 (2002).
Aleixo, A. Historical Diversification of a Terra-Firme Forest Bird Superspecies: a Phylogeographic Perspective on the Role of Different Hypotheses of Amazonian Diversification. Evolution (N. Y). 58(6), 1303–1317 (2004).
Higgins, M. A. et al. Geological control of floristic composition in Amazonian forests. J. Biogeogr. 38, 2136–2149 (2011).
Smith, B. T. et al. The drivers of tropical speciation. Nature 515, 406–409 (2014).
Oliveira, U., Vasconcelos, M. F. & Santos, A. J. Biogeography of Amazon birds: rivers limit species composition, but not areas of endemism. Sci. Rep. 7, 2992 (2017).
Nazareno, A. G., Dick, C. W. & Lohmann, L. G. Wide but not impermeable: Testing the riverine barrier hypothesis for an Amazonian plant species. Mol. Ecol. 38, 42–49 (2017).
Van Roosmalen, M. G. M., Van Roosmalen, T. & Mittermeier, R. A taxonomic review of the titi monkeys, genus Callicebus Thomas, 1903, with the description of two new species, Callicebus bernhardi and Callicebus stephennashi, from Brazilian Amazonia. Neotrop. Primates 10, 1–52 (2002).
Simões, P. I., Lima, A. P., Magnusson, W. E., Hödl, W. & Amézquita, A. Acoustic and morphological differentiation in the frog Allobates femoralis: Relationships with the upper Madeira River and other potential geological barriers. Biotropica 40, 607–614 (2008).
Fernandes, A. M., Gonzalez, J., Wink, M. & Aleixo, A. Multilocus phylogeography of the Wedge-billed Woodcreeper Glyphorynchus spirurus (Aves, Furnariidae) in lowland Amazonia: Widespread cryptic diversity and paraphyly reveal a complex diversification pattern. Mol. Phylogenet. Evol. 66, 270–282 (2013).
Kaefer, I. L., Tsuji-nishikido, B. M., Mota, E. P., Farias, I. P. & Lima, A. P. The Early Stages of Speciation in Amazonian Forest Frogs: Phenotypic Conservatism Despite Strong Genetic Structure. Evol. Biol. 40, 228–245 (2013).
Fecchio, A. et al. Host community similarity and geography shape the diversity and distribution of haemosporidian parasites in Amazonian birds. Ecography (Cop.). https://doi.org/10.1111/ecog.03058 (2017).
Burney, C. W. & Brumfield, R. T. Ecology predicts levels of genetic differentiation in neotropical birds. Am. Nat. 174, 358–368 (2009).
Wollenberg, K. C., Vieites, D. R., Glaw, F. & Vences, M. Speciation in little: the role of range and body size in the diversification of Malagasy mantellid frogs. BMC Evol. Biol. 11, 217 (2011).
Williams, B. K., Nichols, J. D. & Conroy, M. J. Analysis and Management of Animal Populations (Academic Press) 1–817 (Oxford, 2002).
Losos, J. B. & Glor, R. E. Phylogenetic comparative methods and the geography of speciation. Trends Ecol. Evol. 18, 220–227 (2003).
Boughman, J. W. Divergent sexual selection enhances reproductive isolation in sticklebacks. Nature 411, 944–948 (2001).
Maan, M. E. & Seehausen, O. Ecology, sexual selection and speciation. Ecol. Lett. 14, 591–602 (2011).
Rice, W. & Salt, G. The Evolution of Reproductive Isolation as a Correlated Character Under Sympatric Conditions: Experimental Evidence. Evolution 44, 1140–1152 (1990).
Jiggins, C. D., Naisbit, R. E., Coe, R. L. & Mallet, J. Reproductive isolation caused by colour pattern mimicry. Nature 411, 302–305 (2001).
Nagel, L. & Schulter, D. Body Size, Natural Selection, and Speciation in Sticklebacks. Evolution 52, 209–218 (1998).
Dieckmann, U. & Doebeli, M. O. On the origin of species by sympatric speciation. Nature 400, 354–357 (1999).
Turelli, M., Barton, N. H. & Coyne, J. A. Theory and speciation. Trends Ecol. Evol. 16, 330–343 (2001).
Dexter, K. G. et al. Dispersal assembly of rain forest tree communities across the Amazon basin. Proc. Natl. Acad. Sci. 114(10), 2645–2650 (2017).
Gutiérrez, E. E., Boria, R. A. & Anderson, R. P. Can biotic interactions cause allopatry? Niche models, competition, and distributions of South American mouse opossums. Ecography (Cop.). 37, 741–753 (2014).
Elias, S. A. Insects and climate change - fossil evidence from the Rocky Mountains. Bioscience 41, 552–559 (1991).
Elias, S. A. Late Quaternary Zoogeography of the Chihuahuan Desert Insect Fauna, Based on Fossil Records from Packrat Middens. J. Biogeogr. 19, 285–297 (1992).
Coope, G. R. & Wilkins, A. S. The Response of Insect Faunas to Glacial-Interglacial Climatic Fluctuations. Philos. Trans. R. Soc. B Biol. Sci. 344, 19–26 (1994).
Graham, R. W. et al. Spatial Response of Mammals to Late Quaternary Environmental Fluctuations. Science 272, 1601–1606 (1996).
Kaustuv, R., Jablonski, D. & Valentine, J. W. Climate change, species range limits and body size in marine bivalves. Ecol. Lett. 4, 366–370 (2001).
Caro, T. M. & O’Doherty, G. On the use of surrogate species in conservation biology. Conserv. Biol. 13, 805–814 (1999).
Magnusson, W. E. et al. Biodiversity and Integrated Environmental Monitoring (Áttema Editorial) 1–351 (Santo André, 2013).
Michonneau, F., Brown, J. & Winter, D. Rotl: an R package to interact with the Open Tree of Life data. Methods Ecol. Evol. 7, 1476–1481 (2016).
Jetz, W., Thomas, G. H., Joy, J. B., Hartmann, K. & Mooers, A. O. The global diversity of birds in space and time. Nature 491, 444–448 (2012).
Jetz, W. et al. Global Distribution and Conservation of Evolutionary Distinctness in Birds. Curr. Biol. 24, 919–930 (2014).
Rubolini, D., Liker, A., Garamszegi, L. Z., Møller, A. P. & Saino, N. Using the birdtree.org website to obtain robust phylogenies for avian comparative studies: A primer. Curr. Zool. 61, 959–965 (2015).
Acknowledgements
This study was supported by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES; Coordenação de Pesquisas em Biodiversidade – COBIO of the Instituto Nacional de Pesquisas da Amazônia - INPA; Programa de Pós Graduação em Ciências Biológicas (Biologia de Água Doce e Pesca Interior); Santo Antônio Energia – SAE; National Institute for Amazonian Biodiversity (INCT-CENBAM) and the Program for Biodiversity Research in Western Amazonia (PPBio-AmOc). The data were collected by many biologists as part of the environmental-impact studies undertaken by SAE. We especially thank Albertina Lima for indications about the existence of data for various groups.
Author information
Authors and Affiliations
Contributions
S.S.J., C.P.D. and W.E.M. designed the study, S.S.J. analyzed the data with contribution of W.E.M., S.S.J. wrote the first draft of the manuscript, and all authors contributed substantially to revisions.
Corresponding author
Ethics declarations
Competing Interests
The authors declare that they have no competing interests.
Additional information
Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Santorelli, S., Magnusson, W.E. & Deus, C.P. Most species are not limited by an Amazonian river postulated to be a border between endemism areas. Sci Rep 8, 2294 (2018). https://doi.org/10.1038/s41598-018-20596-7
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-018-20596-7
This article is cited by
-
Small mammal diversity of a poorly known and threatened Amazon region, the Tapajós Area of Endemism
Biodiversity and Conservation (2022)
-
Direct and indirect effects of geographic and environmental factors on ant beta diversity across Amazon basin
Oecologia (2022)
-
Microgeographic Morphophysiological Divergence in an Amazonian Soil Mite
Evolutionary Biology (2021)
-
Analysis of the mitochondrial D-Loop reveals that neither river boundaries nor geographic distance structure the fine-scale genetic variation of an Amazonian treefrog
Hydrobiologia (2020)
-
The role of environmental filtering, geographic distance and dispersal barriers in shaping the turnover of plant and animal species in Amazonia
Biodiversity and Conservation (2020)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.