Abstract
The outer membrane of gram-negative bacteria is a permeability barrier that prevents the efficient uptake of molecules with large scaffolds. As a consequence, a number of antibiotic classes are ineffective against gram-negative strains. Herein we carried out a high throughput screen for small molecules that make the outer membrane of Escherichia coli more permeable. We identified MAC13243, an inhibitor of the periplasmic chaperone LolA that traffics lipoproteins from the inner to the outer membrane. We observed that cells were (1) more permeable to the fluorescent probe 1-N-phenylnapthylamine, and (2) more susceptible to large-scaffold antibiotics when sub-inhibitory concentrations of MAC13243 were used. To exclude the possibility that the permeability was caused by an off-target effect, we genetically reconstructed the MAC13243-phenotype by depleting LolA levels using the CRISPRi system.
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Introduction
A distinguishing feature of gram-negative bacteria is their outer membrane, which under normal physiological conditions is an asymmetric bilayer that contains mainly lipopolysaccharides (LPS) in the outer leaflet and phospholipids in the inner leaflet1,2,3. This membrane is essentially an impermeable barrier that separates and protects the cell from the extra-cellular mileu. ß-barrel porins embedded in the outer membrane maintain cellular homeostasis by selecting the chemistry that enters and leaves the cell. It is generally accepted that ß-barrel porins allow, by passive diffusion, the passage of hydrophilic molecules that are less than 600 Da4,5,6. Molecular shape, flexibility and the presence of an ionisable nitrogen group are also important for diffusion through porins7. These exclusion limits enable the uptake of essential nutrients and ions, as well as those antibiotics with small molecular scaffolds. Antibiotics with larger and less favourable scaffolds cannot effectively cross the outer membrane (Fig. 1a). These large-scaffold antibiotics can, in some instances, diffuse through the LPS layer, but the process is inefficient and they are therefore considered ineffective against gram-negative bacteria8,9,10. Examples of large-scaffold antibiotics from four different antibiotic classes are shown in Fig. 1b.
Large-scaffold antibiotics are taken up more efficiently when the biogenesis of the cell envelope is perturbed. For example, in strains with genetic mutations that affect protein trafficking and folding in the cell envelope, or lipopolysaccharide biosynthesis11,12,13,14. A number of small molecules that inhibit the biogenesis of the cell envelope have been discovered (see15,16,17,18,19,20,21,22,23,24 for examples). But to our knowledge, only a few of these have been shown to potentiate large-scaffold antibiotics: (1) polymyxins are cationic polypeptide antibiotics that disrupt the LPS layer15,24,25, (2) loperamide is an anti-diarrhoeal drug (target unknown) that dissipates the inner membrane potential17, (3) A22 is an inhibitor of the actin homologue MreB23. In this study we set out to discover new lead molecules that could be used to make the outer membrane of gram-negative bacteria more permeable to large-scaffold antibiotics.
Results
A high throughput screen for molecules that make E. coli susceptible to vancomycin
We initially carried out a high throughput screen to identify small molecules that make Escherichia coli more susceptible to vancomycin. Vancomycin is a glycopeptide antibiotic that exceeds the exclusion limit of ß-barrel porins and therefore cannot pass the outer membrane. However E. coli is susceptible to vancomycin when proteins involved in cell envelope biogenesis are inhibited; For example, a strain lacking the periplasmic chaperone SurA has a compromised outer membrane and is susceptible to much lower concentrations of vancomycin than a wild type (WT) strain12. Our screen was performed by monitoring the growth of E. coli MC4100 in a 96-well format, in the presence of a sub-lethal concentration of vancomycin and 28,000 small molecules. We reasoned that if a small molecule increased the permeability of the outer membrane, vancomycin would gain access to the periplasm and cell growth would be inhibited (Fig. 2a). The screening conditions we selected were deemed to be robust as we observed a visible growth difference when the WT MC4100 and the vancomycin sensitive ∆surA strain were incubated in the presence of vancomycin at 1/3 the Minimal Inhibitory Concentration (MIC) (Z-score of 0.89; Fig. 2b). The primary screen was initially carried out with 17,500 small molecules from a diverse set of compounds (ChemBridge) at 12.5 µM and in the presence of 100 µg mL−1 of vancomycin (1/5 MIC). As this did not yield any significant hits (data not shown) a second screen was carried out. In the second screen 10,500 small molecules were screened from the CBCS primary screening set at 10 µM in presence of 150 µg mL−1 of vancomycin (1/3 MIC). This second screen indicated that 124 small molecules inhibited the growth of MC4100 by more than 30% relative to a DMSO control (red data points, Fig. 2c). Roughly half of these molecules exhibited antimicrobial activity in the absence of vancomycin and they were not considered further. Only 12 of the 124 small molecules inhibited cell growth in a vancomycin- and dose-dependent manner (Supplementary Figure 1). Six of these molecules were from antibiotic classes that were previously known to function synergistically with vancomycin in gram-negative bacteria23,26,27,28, and this category served as positive controls for the screen (1–6 Fig. 2d). The other six molecules were not previously known to work in combination with vancomycin and they were chosen for follow up experiments (7–12 Fig. 2d). This group included three nucleoside analogues (5-ethynyl-2′-deoxyuridine, zebularine, floxuridine), two molecules that were annotated as antibiotics (carbadox, streptozotocin) and an inhibitor of the periplasmic chaperone LolA (MAC13243)7.
MAC13243 permeabilises the outer membrane of E. coli
We used the fluorescent probe 1-N-phenylnapthylamine (NPN) to determine if the molecules identified in the screen were causing the outer membrane of E. coli to be more permeable. NPN is a small molecule (219 Da) that cannot effectively cross the outer membrane. It is weakly fluorescent in aqueous solution but fluoresces strongly when it binds to phospholipids. This property can be exploited to probe the permeability of the outer membrane29,30: WT cells are weakly fluorescent since NPN is not effectively taken up, but strains with a compromised outer membrane are fluorescent since NPN can access the periplasmic space and the phospholipids of the inner and outer membranes (Fig. 3a). When we carried out NPN uptake assays in cells treated with a sub-lethal concentration of floxuridine, carbadox, streptozotocin and MAC13243, we observed that only cells treated with MAC13243 became fluorescent (Fig. 3b). We did not test 5-ethynyl-2′-deoxyuridine and zebularine since they were deemed to be analogues of floxuridine (Fig. 2d). The amount of fluorescence observed in cells treated with MAC13243 was 15x higher than the DMSO control (Fig. 3b) and was concentration dependent (Fig. 3c). To gauge how permeable the cells treated with MAC13243 had become, we compared the NPN fluorescence values to those seen in cells treated with a sub-inhibitory concentration of colistin, a polymyxin antibiotic that disrupts the LPS layer15,24. We observed that MAC13243-treated cells were more permeable than colistin-treated cells (Fig. 3c vs d). We also compared the NPN fluorescence values of MAC13243-treated cells to strains that were known to be more permeable. This comparison included a deep-rough strain that lacked a glycosyltransferase required for LPS synthesis (∆waaG)12,31 and a strain carrying a deletion in a protein involved in LPS trafficking (lptD4213)32. These data indicated that cells treated with a sub-lethal concentration of MAC13243 were more permeable to NPN than the ∆waaG strain, and less permeable than the lptD4213 strain (Fig. 3e). Taken together these data indicate that a sub-lethal concentration of MAC13243 causes the outer membrane of E. coli to be more permeable to NPN. The molecular reason why compounds 7–11 made cells more susceptible to vancomycin in the original screen remains to be determined.
MAC13243 sensitises E. coli to large-scaffold antibiotics
To determine if the permeability observed in MAC13243-treated cells could be exploited to increase the uptake of large-scaffold antibiotics, we monitored cell growth in the presence of sub-lethal concentrations of vancomycin, rifampicin, erythromycin or novobiocin (Fig. 1b). In these experiments the assay conditions used were similar to those used in the original screen, except that antibiotics were used at ½ MIC. We observed that growth of MC4100 was only slightly perturbed when 10 µM MAC13243 was present, moderately perturbed when the large-scaffold antibiotics were present at ½ MIC, and severely perturbed when both MAC13243 and large-scaffold antibiotics were present (Fig. 4a). The most likely explanation for these data is that 10 µM MAC13243 increased the permeability of the outer membrane and allowed the large-scaffold antibiotics to enter more efficiently.
To quantify how efficiently MAC13243 was working with the large-scaffold antibiotics we carried out chequerboard assays, which are a commonly used methodology to determine if two drugs work synergistically. In the chequerboard assays the assay conditions were slightly different (see methods) and growth inhibition was evaluated in the presence of serial dilutions of MAC13243 and the large-scaffold antibiotics. The data were then used to calculate a Fractional Inhibitory Concentration Index (FICI). Following community guidelines the combinations were deemed to work synergistically if the FICI was ≤ 0.533. Using this mathematical assessment we observed that MAC13243 worked synergistically with erythromycin and novobiocin but not with vancomycin or rifampicin (Fig. 4b). MAC13243 did not work synergistically with the large-scaffold antibiotics when we carried out chequerboard assays with two clinical isolates, E. coli O139 and O141 (data not shown). However it did increase the permeability of these strains to the NPN dye (Supplementary Figure 2). Taken together, these data indicate that MAC13243 is a promising lead molecule that can increase the efficacy of large-scaffold antibiotics, but that the molecular scaffold will need to be optimised if it is to be used as a potentiator.
How does MAC13243 affect outer membrane permeability?
MAC13243 is an inhibitor of LolA21, the periplasmic chaperone that traffics lipoproteins from the inner membrane to the outer membrane13,34. It was initially touted as a promising antibiotic lead because it targets an essential process, is effective against a collection of clinical isolates, and is not a substrate for efflux pumps21. The data presented here indicate that MAC13243 makes E. coli more permeable when used at sub-lethal concentrations. We therefore speculated that the permeable phenotype was caused by partial inhibition of LolA. To explore this possibility we mimicked partial inhibition by reducing the intra-cellular levels of LolA using the CRISPR interference (CRISPRi) technology35. When we induced expression of a single gRNA (sgRNA) specific for lolA together with the nuclease-deficient Cas9 (dCas9) we observed that cell growth was perturbed compared to control cells expressing an sgRNA for the non-essential gene lacZ (encoding ß–galactosidase) (Fig. 5a). This outcome was expected as lolA is essential for viability36 whereas lacZ is not37. When we monitored the permeability of LolA-depleted cells using the NPN assay, we observed that they were more permeable than LacZ-depleted cells (Fig. 5b). We could not confirm that LolA levels were depleted, as we did not have antisera to LolA. But we were able to show that outer membrane lipoproteins and ß-barrel proteins were partially retained at the inner membrane (Fig. 5c). In the control, we observed that an inner membrane protein (PpiD) was enriched in the inner membrane fraction, whereas an outer membrane protein (OmpA), and two lipoproteins (BamB, LptE) were enriched in the outer membrane fraction. However in LolA-depleted cells the outer membrane proteins were enriched in the inner membrane, which is consistent with our hypothesis that LolA levels were partially depleted by CRISPRi. The genetic reconstruction therefore demonstrates the principle that partial depletion of LolA is sufficient to make E. coli more permeable.
Discussion
Antibiotic resistance is a major public health threat38,39,40. And although new antibiotics are urgently needed the antibiotic discovery pipelines are virtually empty, particularly for gram-negative bacteria41,42,43. Given this situation, it has been widely acknowledged that we should find better ways to use the antibiotics that we already have at our disposal40,44,45,46. With this philosophy in mind we set out to identify lead molecules that could make gram-negative bacteria more permeable, and more susceptible to large-scaffold antibiotics. Large-scaffold antibiotics are valuable community resources that were originally discovered as natural products, and decades of synthetic tailoring has created generations of molecules with improved characteristics41. These antibiotics are already in the public domain, but they are not used to treat gram-negative infections because they cannot efficiently cross the outer membrane8,10,47,48,49,50. We suggest that they represent an untapped community resource that could be repurposed to treat gram-negative infections, if there were approaches to increase the permeability of the outer membrane.
Our study identified MAC13243, which has previously been shown to be an inhibitor of the essential periplasmic chaperone LolA21. The previous work had shown that MAC13243 had antibacterial activity against gram-negative bacteria, and the authors suggested that it was a promising lead molecule. We too observed that MAC13243 had antibacterial activity (MIC ranging from 8 to 256 mg mL−1, depending on whether the cells were grown in LB or M9 minimal media). However a subsequent study noted that MAC13243 degrades in aqueous solution, casting doubt over its usefulness51.
In this study we show for the first time, that sub-inhibitory concentrations of MAC13243 can be used to make E. coli more permeable. In our experiments we observed that E. coli cells were more permeable to the fluorescent dye NPN, as well as large-scaffold antibiotics from four different antibiotic classes when treated with sub-inhibitory concentrations of MAC13243. The fact that cells were more permeable to NPN than colistin-treated cells suggests that MAC13243 is effective at inducing a permeable phenotype. A molecular reason for the permeable phenotype is speculated on in Fig. 6. We reason that a sub-lethal concentration of MAC13243 results in partial inhibition of the LolA chaperone. Since LolA traffics proteins from the inner to the outer membrane, partial inhibition will result in retention of lipoproteins at the inner membrane. E. coli encodes around 90 different lipoproteins, many of which have no known function13. However three outer membrane lipoproteins are directly involved in outer membrane biogenesis and are essential for cell viability: LolB inserts lipoproteins into the outer membrane13, BamD inserts ß-barrel proteins into the outer membrane1 and LptE inserts LPS molecules into the outer membrane52. Thus partial inhibition of LolA can simultaneously affect the function of three proteins that are essential for maintaining the integrity of the outer membrane. Indeed, when we depleted the intracellular levels of LolA using CRISPRi, we observed that LptE was partially retained at the inner membrane, and that cells were more permeable to NPN. We were unable to determine if LolA-depletion was sufficient to cause susceptibility to large-scaffold antibiotics, since the CRISPRi system requires two plasmids with different antibiotic selection markers. Nevertheless the experiment indicated that LolA-depletion was sufficient to increase the permeability of E. coli.
Although the high throughput screen carried out in this study is unique, the molecule we identified (MAC13243) is similar to an antibiotic potentiator identified in a previous study23. In the aforementioned study the authors screened 30,000 compounds for their ability to potentiate the activity of novobiocin (also a large scaffold antibiotic). They identified A22 or S-(4-dichlorobenzyl)isothiourea, an inhibitor of the actin-like protein MreB. A22 is structurally similar to the thiourea moiety of MAC13243, which is liberated in aqueous solution as MAC13243 is hydrolysed51 (see S-(4-chlorobenzyl)isothiourea in Fig. 7). MAC13243, its degradation product S-(4-chlorobenzyl)isothiourea and A22 are of the same molecular class and it is therefore not surprising that they can all bind to LolA21,51 and cause the outer membrane to be more permeable to NPN (Fig. 3; Supplementary Figure 3). However it is surprising that two independent studies have identified essentially the same molecule as a potentiator of large-scaffold antibiotics, since there are literally hundreds of target proteins in E. coli. For example, E. coli is more susceptible to vancomycin when one of 60 different proteins is inactivated12.
Can MAC13243 be used as a potentiator of large-scaffold antibiotics? Whilst we observed that a sub-inhibitory concentration of MAC13243 worked synergistically with large-scaffold antibiotics like novobiocin and erythromycin in lab strains of E. coli, it did not work synergistically with larger scaffolds such as rifampicin and vancomycin. Furthermore it did not work synergistically with any of the tested antibiotics in clinical isolates (although we did observe increased permeability). Thus we suggest that MAC13243 will need to be chemically modified if it is to be used clinically as a potentiator of large-scaffold antibiotics. However it may have a number of immediate uses in biotechnology, such as improving the uptake of large labelling dyes or precursor compounds used for the production of complex chemicals in cell factories.
Methods
Bacterial strains, chemicals and media
The E. coli strain MC4100 (F −, [araD139] B/r , Δ(argF-lac)169, λ −, e14-, flhD5301, Δ(fruK-yeiR)725(fruA25), relA1, rpsL150(strR), rbsR22, Δ(fimB-fimE)632(::IS1), deoC1)) was used for all experiments unless otherwise stated. The E. coli O139 and O141 strains were obtained from Klas Udekwu (Stockholm University, Sweden). The BW25113 ΔwaaG and lptD4213 strains were obtained from Göran Widmalm (Stockholm University, Sweden) and Daniel Kahne (Harvard Medical School, USA), respectively. Strains were either grown in LB broth (Amresco, Stockholm) or M9 minimal medium containing 1x M9 minimal salts (BD Difco, Stockholm, Sweden), 0.4% D-glucose (VWR Chemicals, Stockholm, Sweden), 2 mM MgSO4 (Sigma Aldrich, Stockholm, Sweden) and 0.1 mM CaCl2 (Sigma Aldrich, Stockholm, Sweden). Antibiotics and other chemical compounds were purchased from the following manufacturers: Vancomycin, rifampicin, erythromycin, novobiocin and 1-(N-phenylamino)naphthalene (NPN) from Sigma Aldrich (Stockholm, Sweden); MAC13243 from MedChem Express (Stockholm, Sweden); 5-Floxuridine and Streptozotocin from Cayman Chemical (Michigan, USA); Carbadox from Alfa Aesar (Karlsruhe, Germany) and anhydrotetracycline (aTc) from VWR (Stockholm, Sweden).
The High-Throughput Screen
The first part of the primary screen consisted of 17,500 compounds from a diverse set of small molecules (ChemBridge) screened at a final concentration of 12.5 µM. The second part consisted of 10,500 compounds from the CBCS primary screening set (Chemical Biology Consortium Sweden) screened at a final concentration of 10 µM. The compounds were Echo® spotted (Labcyte) directly into 96-well Nunclon™Δ surface plates (Thermo Fisher Scientific, Stockholm, Sweden). A colony of E. coli MC4100 was inoculated into LB broth and incubated overnight at 37 °C with shaking at 200 rpm. The overnight culture was diluted to OD600 = 0.01 and supplemented with 150 μg mL−1 vancomycin (100 µg mL−1 for the first 17,500 compounds). A 200 μL aliquot of diluted bacteria was then added to each well of the pre-spotted 96-well plates. The plates were incubated for 5 h at 37 °C without shaking and the OD600 were recorded with the Synergy H4 plate reader (BioTek). The growth control consisted of 200 μL diluted bacteria with 200 nl of DMSO. The sterility control consisted of 200 μL sterile growth media.
Validation experiments
Selected small molecules were further analysed in dose-response experiments (with and without vancomycin). Briefly, diluted bacteria were prepared as described in the previous section. A 200 μL aliquot of diluted bacteria was either supplemented with or without 150 μg mL−1 vancomycin and added to a 96-well Nunclon™Δ surface plate and then mixed with two-fold serial dilutions of each small molecule at concentrations ranging from 0 - 20 μM. The plates were incubated for 5 h at 37 °C without shaking and the OD600 was recorded.
Minimal Inhibitory Concentration (MIC) determination
The MIC of each antibiotic and small molecule was determined prior to the outer membrane integrity assays. A single colony of E. coli MC4100 was inoculated into either 5 mL LB broth or M9 minimal medium and incubated overnight at 37 °C and 200 rpm. The overnight culture was diluted with sterile growth medium to an OD600 = 0.0005. 198 μL of diluted cells per well were added to a 96-well Nunclon™Δ surface plate and mixed with 2 μL of two-fold serial dilutions of antibiotic or small molecule with a final concentration ranging from 0 to 2048 μg mL−1. After an 18 h incubation at 37 °C without shaking, the samples were transferred to a 96-well Costar plate (VWR, Stockholm, Sweden) and the OD600 was recorded in a SpectraMax M2e Microplate Reader (Molecular Devices, CA, USA). The growth control consisted of 198 μL diluted culture and 2 μL of the corresponding solvent. The sterility control consisted of 200 μL sterile growth medium and served as a background control. The MIC was defined as bacterial growth that was reduced by more than 90% compared to the growth control.
Outer membrane integrity assays
The permeability of the outer membrane was analysed by using the NPN uptake assay as previously described29. Briefly, a colony of E. coli MC4100 was inoculated into 5 mL of M9 media and incubated overnight at 37 °C with shaking at 200 rpm. The overnight culture was diluted with sterile M9 medium to an OD600 = 0.1 and incubated in a 96-well Nunclon™Δ surface plate without shaking at 37 °C until the culture reached an OD600 = 0.5. The cells were harvested by centrifugation (15,000 x g for 2 min), washed twice with assay buffer (5 mM HEPES, 5 mM glucose, pH 7.2) and resuspended in assay buffer to a final OD600 = 1. Then, 100 μL of washed cells and 100 μL of assay buffer containing 20 μM NPN were mixed together and added to a 96-well optical-bottom black plate (Thermo Fisher Scientific, Stockholm, Sweden). Either 2 μL of a chemical compound, or the corresponding solvent, was added to each well and fluorescence was immediately monitored in a SpectraMax Gemini EM microplate reader (Molecular devices, CA, USA) at an excitation wavelength of 350 nm and emission wavelength of 420 nm for 10 min at 30 sec intervals. For each time point, the NPN uptake was calculated using equation (1) where Fobs is the observed fluorescence at a given chemical compound concentration, Fcontrol is the fluorescence of NPN with E. coli cells in the presence of the corresponding solvent and FB is the fluorescence of NPN in the absence of E. coli cells. Data collected over a 10 minutes was averaged.
Susceptibility testing with large-scaffold antibiotics
A single colony of E. coli MC4100 was inoculated into 5 mL of LB broth and incubated overnight at 37 °C with shaking at 200 rpm. The overnight culture was diluted with sterile LB medium to an OD600 = 0.0005. A 196 μL aliquot of diluted cells was added to each well of a 96-well Nunclon™Δ surface plate and mixed with 2 μL of MAC13243 (final concentration of 10 μM) and/or 2 μL of either vancomycin (f.c. 128 μg mL−1), rifampicin (f.c. 8 μg mL−1), erythromycin (f.c. 128 μg mL−1) or novobiocin (f.c. 32 μg mL−1). The growth control contained 198 μL of diluted bacteria and 2 μL of DMSO. The sterility control contained 200 μL sterile growth medium and served as a background control. After an 18 h incubation at 37 °C without shaking, the OD600 was recorded as mentioned previously.
Checkerboard dilution assay
Checkerboard dilution assays were performed to investigate if a given combination of MAC13243 and antibiotic worked synergistically. The protocol was adapted from23. Briefly, an overnight culture of E. coli MC4100 was diluted with sterile M9 medium to an OD600 = 0.0005. A 196 μL aliquot of diluted culture was added to each well of a 96-well Nunclon™Δ surface plate and mixed with either 2 μL of MAC13243 and/or 2 μL of antibiotic with concentrations ranging from 0 to 1024 μg mL−1. After an 18 h incubation at 37 °C without shaking, the OD600 was measured as described previously. The growth control consisted of 196 μL of diluted culture, 2 μL of DMSO and 2 μL of either H2O or EtOH. To evaluate if any given combination was synergistic, equation (2) was used to calculate the FIC (fractional inhibitory concentration) index where FICA is the MIC of drug A in combination with drug B divided by the MIC of drug A alone and FICB is the MIC of drug B in combination with drug A divided by the MIC of drug B alone. The combinations were deemed synergistic (FICI ≤ 0.5), additive (FICI > 0.5 to 1), indifferent (FICI > 1 to < 2) or antagonistic (FICI ≥ 2), as defined by community guidelines33.
CRISPRi
The sgRNA-expressing plasmids were constructed according to53. In short, a protospacer adjacent motif (PAM) sequence (5′-NGG-′3) and adjacent 20 nucleotides were selected on the non-template strand for lacZ and lolA (as close to the start codon as possible). Each sgRNA construct was transformed together with the dCas9-expression vector into E. coli MC4100 and selected on LB agar supplemented with 34 μg mL−1 chloramphenicol and 25 μg mL−1 kanamycin. A colony of each transformed strain was inoculated into 5 mL of LB medium containing the appropriate antibiotics and grown overnight at 37 °C with shaking at 200 rpm. The cultures were then diluted to OD600 = 0.01, supplemented with fresh antibiotics and 5 mL of diluted cultures were added into a 24-well plate (GE Healthcare, Uppsala, Sweden). Cells were incubated at 37 °C with shaking at 200 rpm and expression of dCas9 and the corresponding sgRNA (sgRNA-LacZ or sgRNA-LolA) was induced with 200 ng mL−1 aTc at OD600 = 0.1.
Membrane fractionation and western blot analysis
A single colony of E. coli MC4100 transformed with the dCas9-expression vector and the corresponding sgRNA (sgRNA-LacZ or sgRNA-LolA) was inoculated into 20 ml LB medium (containing the appropriate antibiotics) and incubated overnight at 37 °C with shaking at 200 rpm. The overnight cultures were diluted with sterile LB to an OD600 = 0.01 in a final volume of 1 L. Incubation was continued under the same conditions for 2 h and expression of dCas9 and the corresponding sgRNA was induced with 200 ng mL−1 aTc. After an additional 5 h incubation under the same conditions, cells were harvested from 2 L of culture, by centrifugation for 20 min at 5000 × g at 4 °C. Harvested cells were resuspended in 1 × PBS and subsequently broken by passing three times through an Emulsiflex-C3 (Avestin, Mannheim, Germany). Unbroken cells were pelleted by centrifugation for 20 min at 8000 × g at 4 °C. The membrane fraction was pelleted from the supernatant by ultracentrifugation for 1 h at 270,000 × g at 4 °C. Membrane fractions were resuspended in 1 ml PBS, placed on top of a three-step sucrose gradient (0.77 M, 1.44 M and 2.02 M sucrose) and separated by ultracentrifugation for 16 h at 230,000 × g at 4 °C as described previously54. The inner and outer membrane fractions (IM + OM) were collected, then proteins were separated by 12% SDS-PAGE and blotted onto a nitrocellulose membrane using a semi-dry blotting device (Bio-Rad, Stockholm, Sweden). The nitrocellulose membranes were decorated with antisera, and detection was carried out using the ECL system (Thermo Scientific, Stockholm, Sweden) and a LAS-1000 CCD camera (Fujifilm).
References
Ruiz, N., Kahne, D. & Silhavy, T. J. Advances in understanding bacterial outer-membrane biogenesis. Nat. Rev. Microbiol. 4, 57–66 (2006).
Silhavy, T. J., Kahne, D. & Walker, S. The bacterial cell envelope. Cold Spring Harb. Perspect. Biol. 2, a000414 (2010).
Nikaido, H. & Vaara, M. Molecular basis of bacterial outer membrane permeability. Microbiol. Rev. 49, 1–32 (1985).
Nikaido, H. Molecular basis of bacterial outer membrane permeability revisited. Microbiol. Mol. Biol. Rev. MMBR 67, 593–656 (2003).
Pagès, J.-M., James, C. E. & Winterhalter, M. The porin and the permeating antibiotic: a selective diffusion barrier in Gram-negative bacteria. Nat. Rev. Microbiol. 6, 893–903 (2008).
Masi, M., Réfregiers, M., Pos, K. M. & Pagès, J.-M. Mechanisms of envelope permeability and antibiotic influx and efflux in Gram-negative bacteria. Nat. Microbiol. 2, 17001 (2017).
Richter, M. F. et al. Predictive compound accumulation rules yield a broad-spectrum antibiotic. Nature 545, 299–304 (2017).
Bolla, J.-M. et al. Strategies for bypassing the membrane barrier in multidrug resistant Gram-negative bacteria. FEBS Lett. 585, 1682–1690 (2011).
Cox, G. & Wright, G. D. Intrinsic antibiotic resistance: mechanisms, origins, challenges and solutions. Int. J. Med. Microbiol. IJMM 303, 287–292 (2013).
Zgurskaya, H. I., Löpez, C. A. & Gnanakaran, S. Permeability Barrier of Gram-Negative Cell Envelopes and Approaches To Bypass It. ACS Infect. Dis. 1, 512–522 (2015).
Tamae, C. et al. Determination of antibiotic hypersensitivity among 4,000 single-gene-knockout mutants of Escherichia coli. J. Bacteriol. 190, 5981–5988 (2008).
Liu, A. et al. Antibiotic sensitivity profiles determined with an Escherichia coli gene knockout collection: generating an antibiotic bar code. Antimicrob. Agents Chemother. 54, 1393–1403 (2010).
Okuda, S. & Tokuda, H. Lipoprotein sorting in bacteria. Annu. Rev. Microbiol. 65, 239–259 (2011).
Nichols, R. J. et al. Phenotypic landscape of a bacterial cell. Cell 144, 143–156 (2011).
Aoki, N. et al. Efficacy of colistin combination therapy in a mouse model of pneumonia caused by multidrug-resistant Pseudomonas aeruginosa. J. Antimicrob. Chemother. 63, 534–542 (2009).
Cochrane, S. A. et al. Antimicrobial lipopeptide tridecaptin A1 selectively binds to Gram-negative lipid II. Proc. Natl. Acad. Sci. USA 113, 11561–11566 (2016).
Ejim, L. et al. Combinations of antibiotics and nonantibiotic drugs enhance antimicrobial efficacy. Nat. Chem. Biol. 7, 348–350 (2011).
Kalinin, D. V. & Holl, R. LpxC inhibitors: a patent review (2010–2016). Expert Opin. Ther. Pat. 27, 1227–1250 (2017).
Pärn, K., Eriste, E. & Langel, Ü. The Antimicrobial and Antiviral Applications of Cell-Penetrating Peptides. Methods Mol. Biol. Clifton NJ 1324, 223–245 (2015).
Personne, Y., Curtis, M. A., Wareham, D. W. & Waite, R. D. Activity of the type I signal peptidase inhibitor MD3 against multidrug-resistant Gram-negative bacteria alone and in combination with colistin. J. Antimicrob. Chemother. 69, 3236–3243 (2014).
Pathania, R. et al. Chemical genomics in Escherichia coli identifies an inhibitor of bacterial lipoprotein targeting. Nat. Chem. Biol. 5, 849–856 (2009).
Urfer, M. et al. A Peptidomimetic Antibiotic Targets Outer Membrane Proteins and Disrupts Selectively the Outer Membrane in Escherichia coli. J. Biol. Chem. 291, 1921–1932 (2016).
Taylor, P. L., Rossi, L., De Pascale, G. & Wright, G. D. A forward chemical screen identifies antibiotic adjuvants in Escherichia coli. ACS Chem. Biol. 7, 1547–1555 (2012).
Gordon, N. C., Png, K. & Wareham, D. W. Potent synergy and sustained bactericidal activity of a vancomycin-colistin combination versus multidrug-resistant strains of Acinetobacter baumannii. Antimicrob. Agents Chemother. 54, 5316–5322 (2010).
Vaara, M. Agents that increase the permeability of the outer membrane. Microbiol. Rev. 56, 395–411 (1992).
Zhou, A. et al. Synergistic interactions of vancomycin with different antibiotics against Escherichia coli: trimethoprim and nitrofurantoin display strong synergies with vancomycin against wild-type E. coli. Antimicrob. Agents Chemother. 59, 276–281 (2015).
Day, C. A., Marceau-Day, M. L. & Day, D. F. Increased susceptibility of Pseudomonas aeruginosa to ciprofloxacin in the presence of vancomycin. Antimicrob. Agents Chemother. 37, 2506–2508 (1993).
Donabedian, H. & Andriole, V. T. Synergy of vancomycin with penicillins and cephalosporins against pseudomonas, klebsiella, and serratia. Yale J. Biol. Med. 50, 165–176 (1977).
Helander, I. M. & Mattila-Sandholm, T. Fluorometric assessment of gram-negative bacterial permeabilization. J. Appl. Microbiol. 88, 213–219 (2000).
Eriksson, M., Nielsen, P. E. & Good, L. Cell permeabilization and uptake of antisense peptide-peptide nucleic acid (PNA) into Escherichia coli. J. Biol. Chem. 277, 7144–7147 (2002).
Yethon, J. A., Vinogradov, E., Perry, M. B. & Whitfield, C. Mutation of the lipopolysaccharide core glycosyltransferase encoded by waaG destabilizes the outer membrane of Escherichia coli by interfering with core phosphorylation. J. Bacteriol. 182, 5620–5623 (2000).
Sampson, B. A., Misra, R. & Benson, S. A. Identification and characterization of a new gene of Escherichia coli K-12 involved in outer membrane permeability. Genetics 122, 491–501 (1989).
European Committee for Antimicrobial Susceptibility Testing (EUCAST) of the European Society of Clinical Microbiology and Infectious Dieases (ESCMID). EUCAST Definitive Document E.Def 1.2, May 2000: Terminology relating to methods for the determination of susceptibility of bacteria to antimicrobial agents. Clin. Microbiol. Infect. Off. Publ. Eur. Soc. Clin. Microbiol. Infect. Dis. 6, 503–508 (2000).
Narita, S.-I. & Tokuda, H. Bacterial lipoproteins; biogenesis, sorting and quality control. Biochim. Biophys. Acta https://doi.org/10.1016/j.bbalip.2016.11.009 (2016).
Qi, L. S. et al. Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell 152, 1173–1183 (2013).
Tajima, T., Yokota, N., Matsuyama, S. & Tokuda, H. Genetic analyses of the in vivo function of LolA, a periplasmic chaperone involved in the outer membrane localization of Escherichia coli lipoproteins. FEBS Lett. 439, 51–54 (1998).
Baba, T. et al. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2, 2006.0008 (2006).
Brown, E. D. & Wright, G. D. Antibacterial drug discovery in the resistance era. Nature 529, 336–343 (2016).
Howard, S. J., Catchpole, M., Watson, J. & Davies, S. C. Antibiotic resistance: global response needed. Lancet Infect. Dis. 13, 1001–1003 (2013).
Laxminarayan, R. et al. Antibiotic resistance-the need for global solutions. Lancet Infect. Dis. 13, 1057–1098 (2013).
Fischbach, M. A. & Walsh, C. T. Antibiotics for emerging pathogens. Science 325, 1089–1093 (2009).
Lewis, K. Platforms for antibiotic discovery. Nat. Rev. Drug Discov. 12, 371–387 (2013).
Payne, D. J., Gwynn, M. N., Holmes, D. J. & Pompliano, D. L. Drugs for bad bugs: confronting the challenges of antibacterial discovery. Nat. Rev. Drug Discov. 6, 29–40 (2007).
Bernal, P., Molina-Santiago, C., Daddaoua, A. & Llamas, M. A. Antibiotic adjuvants: identification and clinical use. Microb. Biotechnol. 6, 445–449 (2013).
Cottarel, G. & Wierzbowski, J. Combination drugs, an emerging option for antibacterial therapy. Trends Biotechnol. 25, 547–555 (2007).
Wright, G. D. Antibiotic Adjuvants: Rescuing Antibiotics from Resistance. Trends Microbiol.. https://doi.org/10.1016/j.tim.2016.06.009 (2016).
Hancock, R. E. & Bell, A. Antibiotic uptake into gram-negative bacteria. Eur. J. Clin. Microbiol. Infect. Dis. Off. Publ. Eur. Soc. Clin. Microbiol. 7, 713–720 (1988).
Delcour, A. H. Outer membrane permeability and antibiotic resistance. Biochim. Biophys. Acta 1794, 808–816 (2009).
Krishnamoorthy, G. et al. Breaking the permeability barrier of Escherichia coli by controlled hyperporination of the outer membrane. Antimicrob. Agents Chemother.. https://doi.org/10.1128/AAC.01882-16 (2016).
Denyer, S. P. & Maillard, J.-Y. Cellular impermeability and uptake of biocides and antibiotics in Gram-negative bacteria. J. Appl. Microbiol. 92(Suppl), 35S–45S (2002).
Barker, C. A. et al. Degradation of MAC13243 and studies of the interaction of resulting thiourea compounds with the lipoprotein targeting chaperone LolA. Bioorg. Med. Chem. Lett. 23, 2426–2431 (2013).
Okuda, S., Sherman, D. J., Silhavy, T. J., Ruiz, N. & Kahne, D. Lipopolysaccharide transport and assembly at the outer membrane: the PEZ model. Nat. Rev. Microbiol. 14, 337–345 (2016).
Ronda, C., Pedersen, L. E., Sommer, M. O. A. & Nielsen, A. T. CRMAGE: CRISPR Optimized MAGE Recombineering. Sci. Rep. 6, 19452 (2016).
Schnaitman, C. A. Protein composition of the cell wall and cytoplasmic membrane of Escherichia coli. J. Bacteriol. 104, 890–901 (1970).
Acknowledgements
We are grateful to Lars Nilsson and Spyridon Gkotzis for technical assistance, and Göran Widmalm, Klas Udekwu, Tom Silhavy and Daniel Kahne for gifts of strains and antisera. This work was supported by the Swedish Research Council, the Department of Biochemistry and Biophysics at Stockholm University and the Novo Nordisk Foundation.
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D.O.D. designed the study. C.M., H.G., A.U.E., S.L. and I.L. performed the experiments. D.O.D. S.L., M.H.H.N. and D.O.D. supervised the research. C.M. and D.O.D. wrote the manuscript. All authors approved the final version.
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Muheim, C., Götzke, H., Eriksson, A.U. et al. Increasing the permeability of Escherichia coli using MAC13243. Sci Rep 7, 17629 (2017). https://doi.org/10.1038/s41598-017-17772-6
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DOI: https://doi.org/10.1038/s41598-017-17772-6
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