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UV irradiation to mouse skin decreases hippocampal neurogenesis and synaptic protein expression via HPA axis activation

Scientific Reportsvolume 7, Article number: 15574 (2017) | Download Citation

Abstract

The skin senses external environment, including ultraviolet light (UV). Hippocampus is a brain region that is responsible for memory and emotion. However, changes in hippocampus by UV irradiation to the skin have not been studied. In this study, after 2 weeks of UV irradiation to the mouse skin, we examined molecular changes related to cognitive functions in the hippocampus and activation of the hypothalamic-pituitary-adrenal (HPA) axis. UV exposure to the skin decreased doublecortin-positive immature neurons and synaptic proteins, including N-methyl-D-aspartate receptor 2 A and postsynaptic density protein-95, in the hippocampus. Moreover, we observed that UV irradiation to the skin down-regulated brain-derived neurotrophic factor expression and ERK signaling in the hippocampus, which are known to modulate neurogenesis and synaptic plasticity. The cutaneous and central HPA axes were activated by UV, which resulted in significant increases in serum levels of corticosterone. Subsequently, UV irradiation to the skin activated the glucocorticoid-signaling pathway in the hippocampal dentate gyrus. Interestingly, after 6 weeks of UV irradiation, mice showed depression-like behavior in the tail suspension test. Taken together, our data suggest that repeated UV exposure through the skin may negatively affect hippocampal neurogenesis and synaptic plasticity along with HPA axis activation.

Introduction

Ultraviolet (UV) irradiation is essential for the synthesis of vitamin D1. However, excessive UV irradiation causes skin aging2 and skin cancer3,4. Depending on their wavelength, UV rays are classified as UVA (320–400 nm), UVB (280–320 nm), and UVC (100–280 nm). UVC is absorbed by the ozone layer, and therefore, does not reach the earth’s surface5,6. UVB is mostly absorbed by the epidermis and UVA penetrates more deeply into the dermis7.

The skin is the largest organ of the body and a front-line homeostatic barrier to the external environment8,9. External environment are sensed by the skin and transmitted to the brain10, and changes of the skin status can remotely modulate homeostatic brain function via systemic cytokines11,12. Conversely, skin condition can be changed due to neuropsychological comorbidity13. This skin-brain bidirectional communication regulates local and systemic homeostasis via the cutaneous neuroendocrine system, containing serotoninergic and melatoninergic systems and the hypothalamic-pituitary-adrenal (HPA) axis9.

Stressful stimuli trigger the activation of the HPA axis via the secretion of corticotropin-releasing hormone (CRH) in the hypothalamic paraventricular nucleus. This in turn stimulates the anterior pituitary to release adrenocorticotropic hormone (ACTH). Circulating ACTH binds to melanocortin 2 receptor (MC2R). This leads to the initiation of glucocorticoid (GC) synthesis in the adrenal cortex14. A fully functional HPA axis homolog called the cutaneous HPA axis exists in the skin. Various skin cells, including keratinocytes, melanocytes and outroot sheet cells, express HPA axis elements such as CRH, ACTH, GCs, and their receptors15. GCs, which are steroid hormones and the final products of the HPA axis, are tightly regulated by negative feedback16. GCs circulate in the body and bind to glucocorticoid receptors (GRs), thereby exerting various physiological actions, such as anti-inflammatory, anti-proliferative, and vasoconstrictive effects17. GRs are abundantly expressed in specific brain regions, including the hippocampus, prefrontal cortex, and amygdala in humans and rodents, and are regulated by GCs18.

The hippocampus plays an important role in emotion response and memory consolidation. Hippocampal neurogenesis and synaptic plasticity are closely related to these hippocampal functions19. In adult mammals, two specific brain regions show neurogenesis throughout life: the subventricular zone of the lateral ventricles and the subgranular zone (SGZ) of the dentate gyrus (DG) in the hippocampus20. Newly generated hippocampal neurons from neural progenitor cells (NPCs) in the SGZ can integrate into preexisting neural circuits. The proliferation, survival, and differentiation of NPCs are regulated via various neurotrophic factors and growth factors21. For example, exercise increases neurogenesis and neural plasticity22 via increased brain-derived neurotrophic factor (BDNF)23, insulin-like growth factor-1 (IGF-1)24, and vascular endothelial growth factor25, while stress and aging decrease neurogenesis and neurotrophic factors26,27.

The N-methyl-d-aspartate receptor (NMDAR) and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) are ion channels that are essential for synaptogenesis, experience-dependent synaptic remodeling, and synaptic efficacy28. Cognitive deficits occur when the hippocampal NMDARs or AMPARs are impaired, indicating that NMDAR- and AMPAR-dependent synaptic plasticity is essential for normal learning and memory29,30. Moreover, two synapse marker proteins, post-synaptic density protein 95 (PSD-95) and synaptophysin (SYP), are involved in synaptic signal transmission. PSD-95 plays an important role in synaptic maturation and synaptic plasticity31. SYP, an integral membrane protein of synaptic vesicles, reflects changes in synapse vesicles, and SYP expression is reduced by synaptic dysfunction32.

In stress conditions, the hippocampus undergoes various changes, including morphological changes, decreased adult neurogenesis, and modification in synaptic plasticity via increased GCs33. Recent studies have shown that UV irradiation is an external stressor that leads to increases in the blood levels of GCs in rodents34. However, it is still unknown whether UV irradiation to the skin increases GC levels and has a negative effect on the hippocampus. Therefore, we studied the adverse effects of UV irradiation of mouse skin on adult hippocampal neurogenesis and synaptic protein expression, which may play important roles in emotion-related behaviors.

Results

UV irradiation to mouse skin decreased adult hippocampal neurogenesis

To examine the effects of UV irradiation of mouse skin on hippocampal neurogenesis and neuronal survival, biomarkers for neurogenesis, proliferation, and apoptosis were analyzed after repeated UV irradiation. Immunohistochemistry revealed that 2 weeks of UV irradiation to the skin decreased the number of doublecortin (DCX)-positive immature neurons in the hippocampal DG when compared to that in the sham-irradiated group (83.6 ± 8.2% of control, p = 0.0114, Fig. 1a). However, the number of neurons positive for Ki-67, which is a proliferation marker, was not affected (Fig. 1b). Western blot results also confirmed that UV irradiation to the skin significantly decreased DCX expression in hippocampal lysates (57.2 ± 18.0% of control, p = 0.0003, Fig. 1c). The expression levels of the apoptosis-related protein Bcl-2 and cleaved caspase-3 in hippocampal lysates from UV-irradiated mice were the same as those in the control group. These results show that repeated UV exposure of the skin can lead to decreased hippocampal neurogenesis.

Figure 1
Figure 1

UV irradiation of mouse skin decreased adult hippocampal neurogenesis. Representative immunohistochemical images of (a) DCX-positive and (b) Ki-67-positive cells in the DG. DCX- and Ki-67-positive cells were counted in 6 hippocampal sections from each mouse and in 8 mice from each group, and mean values were calculated. (c) The protein expression levels of DCX, Bcl-2, and cleaved caspase-3 were assessed in hippocampal lysates using Western blot. Relative band density was analyzed using Image J software. Tubulin was used as the endogenous control. The blots are cropped raw images are provided in Supplementary Figure S2. Scale bars: 100 µm. Graphs show means ± SEM (n = 8 mice/group). *P < 0.05 and ***P < 0.001 indicate significant differences when compared to the control group. Bcl-2, B-cell lymphoma 2; C.caspase-3, cleaved caspase-3; DCX, doublecortin; DG, dentate gyrus.

UV irradiation to mouse skin reduced the levels of the synaptic proteins NMDAR2A and PSD-95 in the hippocampus

To investigate the effects of UV irradiation on hippocampal synaptic plasticity, we examined the levels of synaptic proteins, including NMDAR subunits (NMDAR2A and NMDAR2B), AMPAR subunits (GluA1 and GluA2), PSD-95, and SYP in the hippocampus using Western blot (Fig. 2a). Interestingly, 2 weeks of UV irradiation to the skin decreased the hippocampal levels of NMDAR2A and PSD-95 (26.7 ± 18.6% and 40.2 ± 10.1% of control, p = 0.0148 and p = 0.0017, respectively; Fig. 2b). In contrast, the levels of other synaptic proteins did not differ from those of the sham-irradiated group. Our results indicate that UV irradiation to the skin decreases the expression of the synaptic proteins such as NMDAR2A and the complex protein PSD-95.

Figure 2
Figure 2

The expression levels of synaptic proteins NMDAR2A and PSD-95 were reduced by UV irradiation. (a) Representative western blot analysis results, and (b) density graphs of NMDAR2A, NMDAR2B, GluA1, GluA2, PSD-95, and SYP in the DG. Relative band density was analyzed using Image J software and normalized by tubulin. The blots are cropped raw images are provided in Supplementary Figure S3. Graphs show means ± SEM (n = 8 mice/group). *P < 0.05 and ***P < 0.001 indicate significant differences when compared to the control group. GluA1, glutamate receptor 1; GluA2, glutamate receptor 2, NMDAR2A, N-methyl-D-aspartate receptor 2 A; NMDAR2B, N-methyl-D-aspartate receptor 2B; PSD-95, post-synaptic density protein 95; SYP, synaptophysin.

UV irradiation to mouse skin suppressed the hippocampal expression of BDNF and phosphorylated ERK

To further understand how UV irradiation to the skin modulates neurogenesis and synaptic proteins in the hippocampus, we investigated expression changes in neurotropic factors such as BDNF and NGF, and growth factors such as VEGF, IGF-1, and FGF-2 in the hippocampus using RT-qPCR. Interestingly, BDNF mRNA levels were significantly down-regulated (75.4 ± 7.0% of control, p = 0.0003, Fig. 3a), while the mRNA levels of other genes were unchanged. BDNF plays critical roles in the enhancement of neurogenesis, neuroprotection, and synaptic plasticity35. Using Western blot analysis, we observed that skin UV exposure significantly decreased BDNF protein expression in the hippocampus (55.1 ± 18.2% of control, p = 0.0014, Fig. 3b,c). The ERK signaling pathway is activated when BDNF binds to tropomyosin-related kinase B, which is known as a BDNF receptor, and plays an important role in neuronal progenitor maturation and synaptic development36. Consistently, we also found that the levels of phosphorylated ERK were significantly down-regulated in the hippocampus following UV irradiation of the skin (49.5 ± 22.7% of control, p = 0.0140, Fig. 3b,c). Our results suggest that exposure of skin to UV radiation leads to down-regulation of BDNF expression and suppression of ERK activity in the hippocampus, which may result in decreased neurogenesis and synaptic protein expression in the hippocampus.

Figure 3
Figure 3

UV irradiation suppressed the expression of BDNF and phosphorylated ERK in the hippocampus. (a) Neurotropic factors (BDNF, NGF) and growth factors (VEGF, IGF-1, and FGF-2) mRNA expression levels in the hippocampus were analyzed using RT-qPCR. GAPDH was used to normalize the mRNA expression levels. Representative Western blot images and density graphs of (b) BDNF, p-ERK, and t-ERK in the hippocampus. (c) Relative band density was analyzed using image J software. Tubulin and t-ERK were used as endogenous controls, respectively. The blots are cropped raw images are provided in Supplementary Figure S4. Graphs show means ± SEM (n = 8mice/group). *P < 0.05, **P < 0.01, and ***P < 0.001 indicate significant differences when compared to control group. BDNF, brain-derived neurotrophic factor; FGF-2, fibroblast growth factor 2; IGF-1, insulin-like growth factor 1; NGF, nerve growth factor; p-ERK, phosphorylated extracellular signal-related kinase; t-ERK, total extracellular signal-related kinase VEGF, vascular endothelial growth factor.

UV irradiation to mouse skin activated the central and cutaneous HPA axes

Stress-induced GCs are the most potent neurogenesis suppressors37. Moreover, reduced BDNF levels have been reported in chronic corticosterone (CORT) treated rats38. Therefore, we investigated whether UV radiation for 2 weeks could induce HPA axis activation and whether the subsequent increase in CORT levels played a role in the UV irradiation-induced suppression of hippocampal neurogenesis and synaptic protein expression. First, we analyzed the serum levels of CORT using ELISA. CORT concentrations were markedly elevated 12 h after the last UV exposure in the sera of mice irradiated for 2 weeks (248.8 ± 106.9% of control, p = 0.0148, Fig. 4a). Next, to examine central HPA axis activation, we analyzed steroidogenesis in the adrenal glands. When ACTH binds to MC2R, adrenocortical steroidogenesis is triggered and cholesterol is transported into the mitochondria by steroidogenic acute regulatory protein (STAR). In addition, CORT levels are regulated by cytochrome P450 family 11 subfamily b member 1 (CYP11B1), which is involved in the conversion of 11- deoxycorticosterone to CORT in the adrenal cortex39. Interestingly, the mRNA levels of MC2R, STAR, and CYP11B1 were significantly increased (355.8 ± 114.5%, 234.0 ± 64.1%, and 177.0 ± 37.1% of control, p = 0.002, p = 0.0002, p = 0.007, respectively; Fig. 4b) in the adrenal glands of UV-irradiated mice. However, the serum level of ACTH (Fig. 4c) and CRH mRNA level in the hypothalamus (Fig. 4d) were unchanged in the UV-irradiated mice. Since the HPA axis is tightly regulated by a negative feedback mechanism40, the levels of ACTH and CRH in the UV-irradiated group may be due to negative feedback regulation.

Figure 4
Figure 4

UV irradiation stimulated both central and cutaneous HPA axes. Serum levels of (a) CORT and (c) ACTH were measured using ELISA assay. (b) MC2R, STAR, and CYP11B1 mRNA levels in the adrenal glands, and (d) CRH mRNA expression levels in the hypothalamus were measured using RT-qPCR. (e) UCN and POMC expression levels in mouse skin with or without ultraviolet radiation exposure were examined using immunohistochemistry. Scale bars: 50 µm under 400x magnification. (f) The mRNA expression levels of UCN, POMC, MC2R, and CYP11B1 in the skin were analyzed using RT-qPCR. All relative mRNA levels were normalized to those of the endogenous control GAPDH. Graphs show means ± SEM (n = 8mice/group). *P < 0.05, **P < 0.01, and ***P < 0.001 indicate significant differences between groups. ACTH, adrenocorticotropic hormone; CORT, corticosterone; CRH, corticotropin-releasing hormone; CYP11B1, cytochrome P450 family 11 subfamily B member 1; MC2R, melanocortin 2 receptor; POMC, pro-opiomelanocortin; STAR, steroidogenic acute regulatory protein; UCN, urocortin.

The cutaneous HPA axis can contribute to an increase in the level of circulating CORT following acute UV irradiation34. To examine whether the cutaneous HPA axis was activated in our experimental setting, we performed immunohistochemical staining of UV-irradiated mouse skin to evaluate the expression of urocortin (UCN), which is a member of the CRH family, and that of pro-opiomelanocortin (POMC). In UV-irradiated mice, UCN expression was increased in the epidermis, and POMC expression was enhanced in both the epidermis and the dermis in UV-irradiated mice (Fig. 4e). Consistently, mRNA levels of UCN, POMC, MC2R, and CYP11B1 were up-regulated (221.2 ± 121.7%, 184.6 ± 97.9%, 313.0 ± 147.6%, and 178.5 ± 53.1% of control, p = 0.0175, p = 0.0262, p = 0.0041, and p = 0.0111, respectively; Fig. 4f). Our results indicate that repeated UV irradiation to the skin increases the levels of circulating CORT via the activation of the central and cutaneous HPA axes.

UV irradiation of mouse skin induced glucocorticoid receptor activation in the hippocampus

To confirm whether the CORT produced following UV irradiation binds to GRs in the hippocampus, we quantified GR staining in the DG (Fig. 5a). GR is translocated to the nucleus when it is bound by GCs, and acts as a transcription factor41. Our results showed that the numbers of cells with GR-positive nuclei in the DG were markedly increased in the UV-irradiated group (149.7 ± 42.6% of control, p = 0.0411, Fig. 5b). Expression of both GR and arrestin beta 2 (ARRB2) is down-regulated following GR activation42, while tristetraprolin (TTP) expression is up-regulated43. We thus analyzed the expression of GR target genes in the hippocampus. Our results revealed that exposure of the skin to UV radiation significantly reduced GR and ARRB2 mRNA levels and increased TTP mRNA levels in the hippocampus (79.9 ± 12.5%, 67.8 ± 12.5%, and 146.8 ± 45.4% of control, p = 0.0030, p = 0.0006, and p = 0.0370, respectively; Fig. 5c). Our findings suggest that UV irradiation of the skin increases circulating CORT, which may then bind to hippocampal GRs and lead to decreases in hippocampal neurogenesis and synaptic proteins expression.

Figure 5
Figure 5

UV irradiation of mouse skin induced nuclear translocation of GR and modulated the expression levels of its target genes. (a) Representative immunohistochemical images of GR-positive cells under 200x (upper panel) and high magnification (lower panel) of insert area in the hippocampus. Scale bars: 100 µm and 50 µm, respectively. The graphs show (b) GR-positive cell numbers counted in 8 brain sections from each mouse. (c) Relative GR, ARRB2, and TTP mRNA expression levels in the hippocampus were analyzed using RT-qPCR and normalized to GAPDH expression levels. Graphs show means ± SEM (n = 8mice/group). *P < 0.05 and ***P < 0.001 indicate significant differences between groups. ARRB2, arrestin beta 2; GR, glucocorticoid receptor; TTP, tristetraproline.

UV irradiation of mouse skin caused depression-like behavior

Deficits in adult hippocampal neurogenesis and synaptic plasticity in rodents affect the regulation of mood and spatial memory19,44. To investigate whether UV-irradiation to the skin leads to hippocampus-dependent behavioral changes, we performed the tail suspension test (TST). As 2 weeks of UV irradiation was found to be insufficient for producing behavioral change, a 6-week long UV irradiation period was used. A significantly increased immobility time was observed in mice chronically exposed to UV radiation for 6 weeks (129.8 ± 24.4% of control, p = 0.0099, Fig. 6), indicating that chronic UV-irradiation may cause depression-like behavior. Interestingly, only the number of DCX-positive cells was reduced in the 2-week group, while the number of both DCX- and Ki-67-positive cells were decreased in the 6-week group (Supplementary Figure S1). In this experimental condition, UV exposure did not affect memory, as measured using the Y-maze and novel object recognition tests (data was not shown). Thus, our results indicate that chronic excessive UV-irradiation affects hippocampal function in mice.

Figure 6
Figure 6

UV irradiation of mouse skin results in depression-like behavior. To assess a depression-like phenotype (immobility) in UV-irradiated mice, we performed the tail suspension test. UV light was applied to the dorsal skin under anesthesia 3 days a week for 2 or 6 weeks, and the immobility time was measured during the 6 min test session and compared with that of sham-irradiated mice. Graphs show means ± SEM (n = 8 mice/group). *P < 0.05, indicate significant differences compared with control group values. Cont., Sham-UV irradiated group; 2w, 2 weeks UV-irradiated group; 6w, 6 weeks UV-irradiated group.

Discussion

Excessive UV irradiation is considered to be the main cause of skin aging45 and skin cancer4. In addition, chronic UV irradiation results in addiction to UV light due to β-endorphin production46. However, there have been no reports of changes in the hippocampus following repeated exposure of the skin to UV radiation. Here, we report for the first time that repeated exposure of the skin to UV radiation leads to a stress response affecting the hippocampus.

During adult neurogenesis, DCX is expressed in the NPCs. The expression of this protein is down-regulated when the cells begin to express NeuN, which is a marker of mature neurons. Thus, DCX-positive cells are considered as developing neurons47. Various studies have shown that different stressors have different effects on hippocampal neurogenesis because neurogenesis is a multi-step process48,49,50,51. A large number of NPCs undergo programmed cell death after proliferation during a fine-tuning process52. Some stressors, such as predator odor, maternal deprivation, and psychosocial stress stimuli only reduce the numbers of DCX-positive cells without altering their proliferation53,54,55. We found decreased numbers of DCX-positive cells in the SGZ after 2 weeks of UV irradiation, while NPC proliferation and apoptosis were unchanged. Thus, we can deduce that UV radiation inhibits hippocampal neuronal development.

In this study, we observed decreases in synaptic proteins in the UV-irradiated mice hippocampus, such as NMDAR2A and PSD-95. Although each glutamate receptor has a different function, all mediate active signal transduction at synapses. NMDARs are closely associated with the generation of long-term potentiation, a persistent enhancement of synaptic strength by repeated high-frequency stimulation, which is involved in memory formation and synaptic plasticity56,57. PSD-95 is co-localized with NMDARs at excitatory synapses and modulates NMDAR channel gating and surface expression58. Therefore, dysfunction of NMDARs and PSD-95 is closely associated with depressive disorder59,60. We hypothesize that UV irradiation of the skin affects hippocampal functions by modulating the expression of synaptic protein such as NMDAR2A and PSD-95 and by inhibiting hippocampal neurogenesis.

BDNF is the most abundant neurotrophin in the brain61. BDNF levels are reduced in patients and in animal models of depression62,63, and antidepressant drugs increase BDNF levels in the brain64,65. Considering these important roles of BDNF in depression, we examined hippocampal BDNF levels in our model. We observed that hippocampal BDNF and phosphorylated ERK expression was significantly decreased by UV irradiation of the skin. This suggests that UV irradiation affects hippocampus-dependent functions via deficits in neurogenesis, synaptic plasticity, and BDNF expression.

Because of the skin and hippocampus are separated, we hypothesized that CORT, a hormone that negatively affects the hippocampus, acts as a signaling mediator between the skin and the hippocampus. We observed UV irradiation-induced increases in CORT levels in the systemic circulation. An elevated circulating CORT level is a common marker of stress and leads to the suppression of adult neurogenesis. Consistent with the findings in rodents, patients with Cushing’s disease, which leads to excessive secretion of cortisol, have depressive symptoms66 and memory deficits67. Our results demonstrated that the levels of both MC2R and steroidogenesis-related enzyme were up-regulated in the adrenal gland as a stress response after UV irradiation. These results indicated that the UV-induced CORT level increase is due to adrenal gland activation. We found up-regulation of molecules associated not only with the central HPA axis, but also with the cutaneous HPA axis, including UCN, POMC, MC2R, and CYP11B1. Hair follicles also synthesize HPA axis components, and this synthesis varies according to the hair cycle stage68. To exclude change in the cutaneous HPA axis due to variation in the hair cycle stage, we used 6– and 12–week old mice at the telogen stage69. Our results suggest that UV may affect the hippocampus via changes in CORT levels in the skin and adrenal glands.

Abundant evidence indicates that GRs activated by the binding of GCs adversely affect hippocampal neurogenesis, synaptic plasticity, and memory function70,71,72. The direct mechanism of action of GRs involves their dimerization and translocation into the nucleus, where they bind to specific DNA responsive elements, which in turn activate gene expression in a process called transactivation73. Our immunohistochemical staining results confirmed that the number of cells with GR-positive nuclei was higher in the UV-irradiated group than in the non-irradiated group, and that the levels of GR target genes, such as GR, ARRB2, and TTP were modulated after UV irradiation. These results indicate that UV irradiation of the skin led to a stress response, which in turn activated GR dependent signaling pathways in the hippocampus.

In addition to the increase in CORT in UV-irradiated mice, increased inflammatory cytokine expression may also affect brain function. Increased levels of systemic inflammatory cytokines in an animal model of psoriasis have been shown to affect brain homeostasis, leading to depressive behavior11,12. Further investigation is needed to understand the additional mechanisms of UV radiation effects on brain function.

The TST is an appropriate behavioral assessment tool for evaluating depression-like phenotypes74. LPS-injected mice show increased immobility time in the TST, which is a characteristic of depression75. In this study, we did not observe depression-like behavior after 2 weeks of UV irradiation, although hippocampal molecular levels such as BDNF, DCX, NMDAR2A, and PSD-95 were modulated. Therefore, we investigated whether chronic UV exposure could alter behavior. Results of TST conducted after 6 weeks of UV irradiation indicated a statistically significant increase in immobility time. In addition, hippocampal neurogenesis was more severely suppressed in mice irradiated with UV for 6 weeks than in mice irradiated for 2 weeks. These data indicate that excessive repeated UV irradiation to the skin results in hippocampal damage and depressive behavior.

Gamma rays induce cognitive deficit by directly damaging NPCs76. Ablation of neurogenesis has been also demonstrated in mice irradiated with X-rays77,78. These two rays have strong penetration ability and damage cells directly. Unlike these ionizing radiations with high penetration power, most UVB rays are absorbed in the epidermis, and UVA rays are absorbed in the dermis. In this study, we demonstrated that the inhibitory effects of UV radiation on hippocampal neurogenesis and synaptic plasticity are due to indirect effects of UV radiation through activation of the HPA axis.

In conclusion, we report for the first time that chronic UV irradiation of the skin can decrease hippocampal neurogenesis and synaptic signaling, leading to mood changes. We propose that these changes are caused by UV-induced activation of central and cutaneous HPA axis.

Methods

Animals and UV irradiation

All experimental protocols were approved by the Institutional Animal Care and Use Committee (Case Number: 16–0072-S1A0) of the Biomedical Research Institute at Seoul National University Hospital and were performed in accordance with relevant guidelines and regulations. Female C57BL/6 mice were purchased from Orient Experimental Animal Breeding Center (Seoul, Korea) at 5 weeks of age. All animals were housed, 4 per cage, under standard controlled room conditions with food and water available ad libitum. The skin on the backs of the mice was shaved, 2 days prior to UV irradiation using electric clippers, under anesthesia (4% isoflurane). Irradiation was performed using TL20W/12RS UV lamps (Philips, Eindhoven, The Netherlands) with an emission spectrum between 275 and 320 nm. UVC (<290 nm) wavelengths was blocked using Kodacel filter (TA401/407; Kodak, Rochester, NY), which was placed 2 cm in front of UV lamp. UV intensity was measured using a UV meter (model 585100, Waldmann, Villingen-Schwenningen, Germany). In the 2 week irradiation treatment protocol, 200 mJ/cm2 of UV light was applied to the dorsal skin under anesthesia 3 days per week (Monday, Wednesday, and Friday). Chronic excessive UV irradiation (6 week-irradiation treatment) was conducted with 200 mJ/cm2 for the first 2 weeks, 300 mJ/cm2 for the next 2 weeks, and 400 mJ/cm2 for the last 2 weeks.


Immunohistochemistry

Immediately after anesthesia, the dorsal skin79 and brain80 were collected and fixed using 4% formalin for histological studies, as previously reported.


Brain

The brain was removed 12 h after the last UV irradiation, and the left hemisphere was placed in 4% paraformaldehyde overnight, then transferred to a 30% sucrose solution, and kept at 4 °C until the brain tissue was submerged. The brain was embedded in Tissue-Tek OCT compound (Sakura Finetech, Tokyo, Japan) and stored at −80 °C. The brain tissue was sectioned coronally at 40 μm thickness with a freezing microtome; every fifth section was collected and placed in a separate well of a 24-well plate. The brain slice containing the hippocampus was mounted onto silanized slides (Dako, Carpinteria, CA, USA) and dried for 1 h. For immunostaining, the brain slides were rinsed with PBS twice and steamed for antigen retrieval. The sections were washed with PBS and blocked with UltraVision Protein Block (Thermo Scientific, Fremont, USA) for 10 min at room temperature. The slices were incubated overnight at 4 °C with primary antibodies in a diluent buffer containing 1% bovine serum albumin (Sigma-Aldrich, MO, USA) and 1% Triton X-100 in 0.1 M phosphate buffer. After subsequent washing in PBS, the sections were incubated for 1 h at 4 °C with secondary antibody, then incubated with a Vector ABC kit (Vector Laboratories Ltd., CA, USA) and the reaction was visualized with 3,30-diaminobenzidine (DAB; Vector Laboratories Ltd.). The images were acquired with a Leica DM5500B microscope (Leica Microsystems, Wetzlar, Germany). For quantification of the total number of cells in the granular and subgranular zones of the dentate gyrus, the sections were coded, and the counting was performed by an examiner blinded to group allocations.


Skin

The dorsal skin specimens were fixed in 4% paraformaldehyde, embedded in paraffin, and cut into 4 μm thick sections. The sections were stained as described above. For immunofluorescence staining, fluorescence conjugated antibodies were incubated for 1 h at room temperature and the cell nuclei was counterstained with 4′, 6-diamidino-2-phenylindole (DAPI). The images were acquired with a Leica DM5500B microscope (Leica Microsystems).


Immunoblotting analysis

Immunoblotting was performed as previously described80. Briefly, proteins in hippocampal lysates were separated using SDS-PAGE and transferred to membranes. The membranes were blocked and incubated with the appropriate primary antibodies (Supplementary Table S1). After incubation with secondary antibody, the immune complexes were visualized using an ECL detection system (GE Healthcare, Buckinghamshire, UK).


ELISA

The mice were sacrificed and blood was immediately collected in serum separator tubes (BD Microtainer Tubes, Franklin Lakes, NJ). Serum was obtained by centrifugation at 3,500 g for 20 min at 4 °C. The serum was snap-frozen in liquid nitrogen and then stored at −70 °C until the time of the measurements. The serum was thawed and diluted 1:100 to measure CORT and 1:15 to measure ACTH. We performed the assay according to the manufacturers’ protocols using commercially available kits (Supplementary Table S2).


RNA extraction and RT-qPCR

Total RNA was extracted from the dorsal skin, adrenal glands, hippocampus, and hypothalamus using RNAiso Plus (Takara Bio Inc., Shiga, Japan). Total RNA (2 μg) was reverse transcribed to cDNA and RT-qPCR was performed using the appropriate primers (Supplementary Table S3) as described previously80.


Tail-suspension test (TST)

Mice were individually suspended by the tail, using adhesive tape, at least 20 cm above the surface. At each test session, movements of the mice were recorded for subsequent analysis. Immobility during the entire 6 min test session was measured as previously validated81. Immobility time (depression-like behavior) was defined as the time when the animal stopped struggling and was completely motionless. Data analysis was performed by two trained researchers blinded to group allocations.


Statistical analysis

Statistical analyses were performed using SPSS 22.0 software (IBM, Armonk, NY). Differences among groups were analyzed using Mann-Whitney tests. P-values < 0.05 were considered statistically significant.

Additional information

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References

  1. 1.

    MacLaughlin, J. & Holick, M. F. Aging decreases the capacity of human skin to produce vitamin D3. J Clin Invest 76, 1536–1538, https://doi.org/10.1172/JCI112134 (1985).

  2. 2.

    Amaro-Ortiz, A., Yan, B. & D’Orazio, J. A. Ultraviolet radiation, aging and the skin: prevention of damage by topical cAMP manipulation. Molecules 19, 6202–6219, https://doi.org/10.3390/molecules19056202 (2014).

  3. 3.

    de Gruijl, F. R. Photocarcinogenesis: UVA vs. UVB radiation. Skin Pharmacol Appl Skin Physiol 15, 316–320, 64535 (2002).

  4. 4.

    Rigel, D. S. Cutaneous ultraviolet exposure and its relationship to the development of skin cancer. J Am Acad Dermatol 58, S129–132, https://doi.org/10.1016/j.jaad.2007.04.034 (2008).

  5. 5.

    Lautenschlager, S., Wulf, H. C. & Pittelkow, M. R. Photoprotection. Lancet 370, 528–537, https://doi.org/10.1016/S0140-6736(07)60638-2 (2007).

  6. 6.

    Wong, W. C., Wu, J. Y. & Benzie, I. F. Photoprotective potential of Cordyceps polysaccharides against ultraviolet B radiation-induced DNA damage to human skin cells. Br J Dermatol 164, 980–986, https://doi.org/10.1111/j.1365-2133.2010.10201.x (2011).

  7. 7.

    Gupta, A., Avci, P., Dai, T., Huang, Y. Y. & Hamblin, M. R. Ultraviolet Radiation in Wound Care: Sterilization and Stimulation. Adv Wound Care (New Rochelle) 2, 422–437, https://doi.org/10.1089/wound.2012.0366 (2013).

  8. 8.

    Elias, P. M. & Choi, E. H. Interactions among stratum corneum defensive functions. Exp Dermatol 14, 719–726, https://doi.org/10.1111/j.1600-0625.2005.00363.x (2005).

  9. 9.

    Slominski, A. T. et al. Sensing the environment: regulation of local and global homeostasis by the skin’s neuroendocrine system. Adv Anat Embryol Cell Biol 212(v, vii), 1–115 (2012).

  10. 10.

    Roosterman, D., Goerge, T., Schneider, S. W., Bunnett, N. W. & Steinhoff, M. Neuronal control of skin function: the skin as a neuroimmunoendocrine organ. Physiological reviews 86, 1309–1379, https://doi.org/10.1152/physrev.00026.2005 (2006).

  11. 11.

    Nadeem, A. et al. IL-17A causes depression-like symptoms via NFkappaB and p38MAPK signaling pathways in mice: Implications for psoriasis associated depression. Cytokine 97, 14–24, https://doi.org/10.1016/j.cyto.2017.05.018 (2017).

  12. 12.

    McColl, A., Thomson, C. A., Nerurkar, L., Graham, G. J. & Cavanagh, J. TLR7-mediated skin inflammation remotely triggers chemokine expression and leukocyte accumulation in the brain. J Neuroinflammation 13, 102, https://doi.org/10.1186/s12974-016-0562-2 (2016).

  13. 13.

    Rodriguez-Vallecillo, E. & Woodbury-Farina, M. A. Dermatological manifestations of stress in normal and psychiatric populations. Psychiatr Clin North Am 37, 625–651, https://doi.org/10.1016/j.psc.2014.08.009 (2014).

  14. 14.

    Laryea, G., Muglia, L., Arnett, M. & Muglia, L. J. Dissection of glucocorticoid receptor-mediated inhibition of the hypothalamic-pituitary-adrenal axis by gene targeting in mice. Front Neuroendocrinol 36, 150–164, https://doi.org/10.1016/j.yfrne.2014.09.002 (2015).

  15. 15.

    Slominski, A., Wortsman, J., Tuckey, R. C. & Paus, R. Differential expression of HPA axis homolog in the skin. Mol Cell Endocrinol 265–266, 143–149, https://doi.org/10.1016/j.mce.2006.12.012 (2007).

  16. 16.

    Mizoguchi, K., Ishige, A., Aburada, M. & Tabira, T. Chronic stress attenuates glucocorticoid negative feedback: involvement of the prefrontal cortex and hippocampus. Neuroscience 119, 887–897 (2003).

  17. 17.

    Liu, D. et al. A practical guide to the monitoring and management of the complications of systemic corticosteroid therapy. Allergy Asthma Clin Immunol 9, 30, https://doi.org/10.1186/1710-1492-9-30 (2013).

  18. 18.

    Wang, Q. et al. Glucocorticoid receptor protein expression in human hippocampus; stability with age. Neurobiol Aging 34, 1662–1673, https://doi.org/10.1016/j.neurobiolaging.2012.11.019 (2013).

  19. 19.

    Becker, S. & Wojtowicz, J. M. A model of hippocampal neurogenesis in memory and mood disorders. Trends Cogn Sci 11, 70–76, https://doi.org/10.1016/j.tics.2006.10.013 (2007).

  20. 20.

    Zhao, C., Deng, W. & Gage, F. H. Mechanisms and functional implications of adult neurogenesis. Cell 132, 645–660, https://doi.org/10.1016/j.cell.2008.01.033 (2008).

  21. 21.

    Oliveira, S. L. et al. Functions of neurotrophins and growth factors in neurogenesis and brain repair. Cytometry A 83, 76–89, https://doi.org/10.1002/cyto.a.22161 (2013).

  22. 22.

    Heo, Y. M. et al. Treadmill exercise ameliorates disturbance of spatial learning ability in scopolamine-induced amnesia rats. J Exerc Rehabil 10, 155–161, https://doi.org/10.12965/jer.140110 (2014).

  23. 23.

    Gomez-Pinilla, F., Vaynman, S. & Ying, Z. Brain-derived neurotrophic factor functions as a metabotrophin to mediate the effects of exercise on cognition. Eur J Neurosci 28, 2278–2287, https://doi.org/10.1111/j.1460-9568.2008.06524.x (2008).

  24. 24.

    Trejo, J. L., Llorens-Martin, M. V. & Torres-Aleman, I. The effects of exercise on spatial learning and anxiety-like behavior are mediated by an IGF-I-dependent mechanism related to hippocampal neurogenesis. Mol Cell Neurosci 37, 402–411, https://doi.org/10.1016/j.mcn.2007.10.016 (2008).

  25. 25.

    Fabel, K. et al. VEGF is necessary for exercise-induced adult hippocampal neurogenesis. Eur J Neurosci 18, 2803–2812 (2003).

  26. 26.

    Lai, M., Hibberd, C. J., Gluckman, P. D. & Seckl, J. R. Reduced expression of insulin-like growth factor 1 messenger RNA in the hippocampus of aged rats. Neurosci Lett 288, 66–70 (2000).

  27. 27.

    Lee, E. & Son, H. Adult hippocampal neurogenesis and related neurotrophic factors. BMB reports 42, 239–244 (2009).

  28. 28.

    Collingridge, G. L., Isaac, J. T. & Wang, Y. T. Receptor trafficking and synaptic plasticity. Nat Rev Neurosci 5, 952–962, https://doi.org/10.1038/nrn1556 (2004).

  29. 29.

    Tsien, J. Z., Huerta, P. T. & Tonegawa, S. The essential role of hippocampal CA1 NMDA receptor-dependent synaptic plasticity in spatial memory. Cell 87, 1327–1338 (1996).

  30. 30.

    Reisel, D. et al. Spatial memory dissociations in mice lacking GluR1. Nat Neurosci 5, 868–873, https://doi.org/10.1038/nn910 (2002).

  31. 31.

    El-Husseini, A. E., Schnell, E., Chetkovich, D. M., Nicoll, R. A. & Bredt, D. S. PSD-95 involvement in maturation oif excitatory synapses. Science 290, 1364–1368 (2000).

  32. 32.

    Liu, J. et al. Amyloid-beta induces caspase-dependent loss of PSD-95 and synaptophysin through NMDA receptors. J Alzheimers Dis 22, 541–556, https://doi.org/10.3233/JAD-2010-100948 (2010).

  33. 33.

    van Ast, V. A. et al. Modulatory mechanisms of cortisol effects on emotional learning and memory: novel perspectives. Psychoneuroendocrinology 38, 1874–1882, https://doi.org/10.1016/j.psyneuen.2013.06.012 (2013).

  34. 34.

    Skobowiat, C. & Slominski, A. T. UVB Activates Hypothalamic-Pituitary-Adrenal Axis in C57BL/6 Mice. The Journal of investigative dermatology 135, 1638–1648, https://doi.org/10.1038/jid.2014.450 (2015).

  35. 35.

    Begni, V., Riva, M. A. & Cattaneo, A. Cellular and molecular mechanisms of the brain-derived neurotrophic factor in physiological and pathological conditions. Clin Sci (Lond) 131, 123–138, https://doi.org/10.1042/CS20160009 (2017).

  36. 36.

    Yoshii, A. & Constantine-Paton, M. Postsynaptic BDNF-TrkB signaling in synapse maturation, plasticity, and disease. Developmental neurobiology 70, 304–322, https://doi.org/10.1002/dneu.20765 (2010).

  37. 37.

    Diniz, L. et al. Effects of chronic treatment with corticosterone and imipramine on fos immunoreactivity and adult hippocampal neurogenesis. Behavioural brain research 238, 170–177, https://doi.org/10.1016/j.bbr.2012.10.024 (2013).

  38. 38.

    Jacobsen, J. P. & Mork, A. Chronic corticosterone decreases brain-derived neurotrophic factor (BDNF) mRNA and protein in the hippocampus, but not in the frontal cortex, of the rat. Brain Res 1110, 221–225, https://doi.org/10.1016/j.brainres.2006.06.077 (2006).

  39. 39.

    Payne, A. H. & Hales, D. B. Overview of steroidogenic enzymes in the pathway from cholesterol to active steroid hormones. Endocr Rev 25, 947–970, https://doi.org/10.1210/er.2003-0030 (2004).

  40. 40.

    Gomez, F., De Kloet, E. R. & Armario, A. Glucocorticoid negative feedback on the HPA axis in five inbred rat strains. The American journal of physiology 274, R420–427 (1998).

  41. 41.

    Sun, H., Sheveleva, E. & Chen, Q. M. Corticosteroids induce cyclooxygenase 1 expression in cardiomyocytes: role of glucocorticoid receptor and Sp3 transcription factor. Molecular endocrinology 22, 2076–2084, https://doi.org/10.1210/me.2007-0302 (2008).

  42. 42.

    Kadmiel, M. & Cidlowski, J. A. Glucocorticoid receptor signaling in health and disease. Trends Pharmacol Sci 34, 518–530, https://doi.org/10.1016/j.tips.2013.07.003 (2013).

  43. 43.

    Meyer, U., van Kampen, M., Isovich, E., Flugge, G. & Fuchs, E. Chronic psychosocial stress regulates the expression of both GR and MR mRNA in the hippocampal formation of tree shrews. Hippocampus 11, 329–336, https://doi.org/10.1002/hipo.1047 (2001).

  44. 44.

    Sahay, A. & Hen, R. Adult hippocampal neurogenesis in depression. Nat Neurosci 10, 1110–1115, https://doi.org/10.1038/nn1969 (2007).

  45. 45.

    Berneburg, M., Plettenberg, H. & Krutmann, J. Photoaging of human skin. Photodermatol Photoimmunol Photomed 16, 239–244 (2000).

  46. 46.

    Fell, G. L., Robinson, K. C., Mao, J., Woolf, C. J. & Fisher, D. E. Skin beta-endorphin mediates addiction to UV light. Cell 157, 1527–1534, https://doi.org/10.1016/j.cell.2014.04.032 (2014).

  47. 47.

    Couillard-Despres, S. et al. Doublecortin expression levels in adult brain reflect neurogenesis. Eur J Neurosci 21, 1–14, https://doi.org/10.1111/j.1460-9568.2004.03813.x (2005).

  48. 48.

    Gage, F. H. et al. Survival and differentiation of adult neuronal progenitor cells transplanted to the adult brain. Proc Natl Acad Sci USA 92, 11879–11883 (1995).

  49. 49.

    Kornack, D. R. & Rakic, P. Continuation of neurogenesis in the hippocampus of the adult macaque monkey. Proc Natl Acad Sci USA 96, 5768–5773 (1999).

  50. 50.

    Gould, E., Tanapat, P., Rydel, T. & Hastings, N. Regulation of hippocampal neurogenesis in adulthood. Biol Psychiatry 48, 715–720 (2000).

  51. 51.

    Lajud, N. & Torner, L. Early life stress and hippocampal neurogenesis in the neonate: sexual dimorphism, long term consequences and possible mediators. Front Mol Neurosci 8, 3, https://doi.org/10.3389/fnmol.2015.00003 (2015).

  52. 52.

    Biebl, M., Cooper, C. M., Winkler, J. & Kuhn, H. G. Analysis of neurogenesis and programmed cell death reveals a self-renewing capacity in the adult rat brain. Neurosci Lett 291, 17–20 (2000).

  53. 53.

    Thomas, R. M., Urban, J. H. & Peterson, D. A. Acute exposure to predator odor elicits a robust increase in corticosterone and a decrease in activity without altering proliferation in the adult rat hippocampus. Exp Neurol 201, 308–315, https://doi.org/10.1016/j.expneurol.2006.04.010 (2006).

  54. 54.

    Thomas, R. M., Hotsenpiller, G. & Peterson, D. A. Acute psychosocial stress reduces cell survival in adult hippocampal neurogenesis without altering proliferation. J Neurosci 27, 2734–2743, https://doi.org/10.1523/JNEUROSCI.3849-06.2007 (2007).

  55. 55.

    Oomen, C. A. et al. Opposite effects of early maternal deprivation on neurogenesis in male versus female rats. PLoS One 4, e3675, https://doi.org/10.1371/journal.pone.0003675 (2009).

  56. 56.

    Vuyst, A., Vanbelle, M., Joassart, J. M., Vervack, W. & Foulon, M. [Effect of energy level of the ration on the composition of rumen fluid and blood plasma in sheep]. Z Tierphysiol Tierernahr Futtermittelkd 38, 35–42 (1977).

  57. 57.

    Gerrow, K. & Triller, A. Synaptic stability and plasticity in a floating world. Curr Opin Neurobiol 20, 631–639, https://doi.org/10.1016/j.conb.2010.06.010 (2010).

  58. 58.

    Lin, Y., Skeberdis, V. A., Francesconi, A., Bennett, M. V. & Zukin, R. S. Postsynaptic density protein-95 regulates NMDA channel gating and surface expression. J Neurosci 24, 10138–10148, https://doi.org/10.1523/JNEUROSCI.3159-04.2004 (2004).

  59. 59.

    Trullas, R. & Skolnick, P. Functional antagonists at the NMDA receptor complex exhibit antidepressant actions. Eur J Pharmacol 185, 1–10 (1990).

  60. 60.

    Doucet, M. V., Levine, H., Dev, K. K. & Harkin, A. Small-molecule inhibitors at the PSD-95/nNOS interface have antidepressant-like properties in mice. Neuropsychopharmacology 38, 1575–1584, https://doi.org/10.1038/npp.2013.57 (2013).

  61. 61.

    Murer, M. G., Yan, Q. & Raisman-Vozari, R. Brain-derived neurotrophic factor in the control human brain, and in Alzheimer’s disease and Parkinson’s disease. Prog Neurobiol 63, 71–124 (2001).

  62. 62.

    Karege, F. et al. Low brain-derived neurotrophic factor (BDNF) levels in serum of depressed patients probably results from lowered platelet BDNF release unrelated to platelet reactivity. Biol Psychiatry 57, 1068–1072, https://doi.org/10.1016/j.biopsych.2005.01.008 (2005).

  63. 63.

    Duman, R. S. & Monteggia, L. M. A neurotrophic model for stress-related mood disorders. Biol Psychiatry 59, 1116–1127, https://doi.org/10.1016/j.biopsych.2006.02.013 (2006).

  64. 64.

    Aydemir, O., Deveci, A. & Taneli, F. The effect of chronic antidepressant treatment on serum brain-derived neurotrophic factor levels in depressed patients: a preliminary study. Prog Neuropsychopharmacol Biol Psychiatry 29, 261–265, https://doi.org/10.1016/j.pnpbp.2004.11.009 (2005).

  65. 65.

    Liu, D. et al. Resveratrol reverses the effects of chronic unpredictable mild stress on behavior, serum corticosterone levels and BDNF expression in rats. Behavioural brain research 264, 9–16, https://doi.org/10.1016/j.bbr.2014.01.039 (2014).

  66. 66.

    Chen, Y. F., Li, Y. F., Chen, X. & Sun, Q. F. Neuropsychiatric disorders and cognitive dysfunction in patients with Cushing’s disease. Chin Med J (Engl) 126, 3156–3160 (2013).

  67. 67.

    Gold, P. W. et al. Responses to corticotropin-releasing hormone in the hypercortisolism of depression and Cushing’s disease. Pathophysiologic and diagnostic implications. N Engl J Med 314, 1329–1335, https://doi.org/10.1056/NEJM198605223142101 (1986).

  68. 68.

    Ito, N. et al. Human hair follicles display a functional equivalent of the hypothalamic-pituitary-adrenal axis and synthesize cortisol. FASEB J 19, 1332–1334, https://doi.org/10.1096/fj.04-1968fje (2005).

  69. 69.

    Muller-Rover, S. et al. A comprehensive guide for the accurate classification of murine hair follicles in distinct hair cycle stages. The Journal of investigative dermatology 117, 3–15, https://doi.org/10.1046/j.0022-202x.2001.01377.x (2001).

  70. 70.

    Zhang, K. et al. Baicalin promotes hippocampal neurogenesis via SGK1- and FKBP5-mediated glucocorticoid receptor phosphorylation in a neuroendocrine mouse model of anxiety/depression. Scientific reports 6, 30951, https://doi.org/10.1038/srep30951 (2016).

  71. 71.

    Anacker, C. et al. Glucocorticoid-related molecular signaling pathways regulating hippocampal neurogenesis. Neuropsychopharmacology 38, 872–883, https://doi.org/10.1038/npp.2012.253 (2013).

  72. 72.

    Kim, J. J. & Diamond, D. M. The stressed hippocampus, synaptic plasticity and lost memories. Nat Rev Neurosci 3, 453–462, https://doi.org/10.1038/nrn849 (2002).

  73. 73.

    Oakley, R. H. & Cidlowski, J. A. The biology of the glucocorticoid receptor: new signaling mechanisms in health and disease. J Allergy Clin Immunol 132, 1033–1044, https://doi.org/10.1016/j.jaci.2013.09.007 (2013).

  74. 74.

    Can, A. et al. The tail suspension test. J Vis Exp, e3769, https://doi.org/10.3791/3769 (2012).

  75. 75.

    Dunn, A. J. & Swiergiel, A. H. Effects of interleukin-1 and endotoxin in the forced swim and tail suspension tests in mice. Pharmacol Biochem Behav 81, 688–693, https://doi.org/10.1016/j.pbb.2005.04.019 (2005).

  76. 76.

    Oh, S. B. et al. Baicalein attenuates impaired hippocampal neurogenesis and the neurocognitive deficits induced by gamma-ray radiation. Br J Pharmacol 168, 421–431, https://doi.org/10.1111/j.1476-5381.2012.02142.x (2013).

  77. 77.

    Suarez-Pereira, I. & Carrion, A. M. Updating stored memory requires adult hippocampal neurogenesis. Sci Rep 5, 13993, https://doi.org/10.1038/srep13993 (2015).

  78. 78.

    Sun, A. M. et al. X-ray irradiation promotes apoptosis of hippocampal neurons through up-regulation of Cdk5 and p25. Cancer Cell Int 13, 47, https://doi.org/10.1186/1475-2867-13-47 (2013).

  79. 79.

    Kim, J. E. et al. Oral Supplementation with Cocoa Extract Reduces UVB-Induced Wrinkles in Hairless Mouse Skin. The Journal of investigative dermatology 136, 1012–1021, https://doi.org/10.1016/j.jid.2015.11.032 (2016).

  80. 80.

    Bae, J. S. et al. Lycopersicon esculentum Extract Enhances Cognitive Function and Hippocampal Neurogenesis in Aged Mice. Nutrients 8, https://doi.org/10.3390/nu8110679 (2016).

  81. 81.

    Bergner, C. L. et al. Mouse Models for Studying Depression-Like States and Antidepressant Drugs. Methods Mol Biol 1438, 255–269, https://doi.org/10.1007/978-1-4939-3661-8_15 (2016).

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Acknowledgements

This research was supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI14C1277).

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Author notes

  1. Mira Han, Jae-Jun Ban and Jung-Soo Bae contributed equally to this work.

Affiliations

  1. Department of Biomedical Sciences, Seoul National University Graduate School, Seoul, South Korea

    • Mira Han
    • , Jung-Soo Bae
    •  & Jin Ho Chung
  2. Department of Dermatology, Seoul National University College of Medicine, Seoul, South Korea

    • Mira Han
    • , Jae-Jun Ban
    • , Jung-Soo Bae
    • , Chang-Yup Shin
    • , Dong Hun Lee
    •  & Jin Ho Chung
  3. Institute of Human-Environment Interface Biology, Medical Research Center, Seoul National University, Seoul, South Korea

    • Mira Han
    • , Jae-Jun Ban
    • , Jung-Soo Bae
    • , Chang-Yup Shin
    • , Dong Hun Lee
    •  & Jin Ho Chung
  4. Institute on Aging, Seoul National University, Seoul, 03080, Republic of Korea

    • Mira Han
    • , Jae-Jun Ban
    • , Jung-Soo Bae
    • , Dong Hun Lee
    •  & Jin Ho Chung

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Contributions

M.H., J.-S.B., J.H.C. designed study. M.H., J.-J.B., J.-S.B. C.-Y.S. performed the experiments. M.H., J.-J.B., D.H.L. were involved in drafting the article. J.H.C. had full access to all the data, and take full responsibility for the integrity of data and the accuracy of data analysis.

Competing Interests

The authors declare that they have no competing interests.

Corresponding author

Correspondence to Jin Ho Chung.

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