Peatlands perform important ecosystem functions, such as carbon storage and nutrient retention, which are affected, among other factors, by vegetation and peat decomposition. The availability of silicon (Si) in peatlands differs strongly, ranging from <1 to >25 mg L−1. Since decomposition of organic material was recently shown to be accelerated by Si, the aim of this study was to examine how Si influences decomposition of carbon and nutrient and toxicant mobilization in peatlands. We selected a fen site in Northern Bavaria with naturally bioavailable Si pore water concentrations of 5 mg/L and conducted a Si addition experiment. At a fourfold higher Si availability, dissolved organic carbon, carbon dioxide, and methane concentrations increased significantly. Furthermore, dissolved nitrogen, phosphorus, iron, manganese, cobalt, zinc, and arsenic concentrations were significantly higher under high Si availability. This enhanced mobilization may result from Si competing for binding sites but also from stronger reducing conditions, caused by accelerated respiration. The stronger reducing conditions also increased reduction of arsenate to arsenite and thus the mobility of this toxicant. Hence, higher Si availability is suggested to decrease carbon storage and increase nutrient and toxicant mobility in peatland ecosystems.
Peatlands are important ecosystems with regard to carbon storage. Although covering only a small fraction of the land surface (3%), they store one third of the world’s soil carbon stock (~550Pg)1, which corresponds to half of the CO2 in the atmosphere2. Natural peatlands also provide the largest natural source of the potent greenhouse gas (GHG) CH4, yet, they are considered to have an attenuating effect on global warming by a net carbon fixation3, acting as net carbon sinks. However, land use change, such as deforestation, burning, and drainage has turned many peatlands into carbon sources due to e.g. increased peat decomposition4. Besides carbon storage, peatlands perform also other ecosystem functions such as nutrient retention and water quality regulation5. Nutrient retention and water quality regulation are closely linked as peatlands retain atmospherically or agriculturally deposited nitrogen (N) and phosphorus (P), improving the water quality of downstream ecosystems6. Furthermore, peatlands accumulate sulphur7, organic pollutants, toxic metals8, such as arsenic (As), but release dissolved organic carbon9 (DOC) depending on the peat decomposition and hydrology, thus additionally affecting the water quality.
Carbon turnover and nutrient retention in peatlands is a result of the budget of inputs and outputs controlled e.g. by the rate of peat accumulation versus peat decomposition. Among other factors, the peat decomposition rate is influenced by temperature, which controls the activity of decomposing microbes and extracellular enzymes, and by the water table level, which predominantly alternates redox conditions. Under waterlogged, anaerobic conditions peat decomposition is slow, whereas dry aerobic conditions accelerate peat decomposition and cause increased carbon losses10. Under anaerobic conditions, elements bound to redox labile sorbents such as iron phases are released into the solution as the sorbents are reduced11. This element mobilization due to lowering of the redox potential has been demonstrated also for toxic elements such as As. The As speciation can thereby be used to elucidate dynamics in biogeochemical conditions, because of the shifts in predominant As species depending on changes in redox conditions12. Another controlling factor for peat decomposition is the peat nutrient content, which depends on litter quality, but also on exogenous inputs such as atmospheric deposition and input by waters that are enriched by N and P due to agricultural fertilization. Regarding nutrient content of peat, previous research focused mainly on N and P availability as important factors13. However, for grass dominated peat ecosystems, silicon (Si) was recently shown to be important for stability of litter, because increased Si contents increase decomposition14,15,16.
Peatlands and other terrestrial ecosystems represent large reservoirs and filters for Si, controlling the Si transfer to the oceans17. Land use change during the last 250 years has decreased Si availability in soils by increasing export and decreasing Si storage due to higher erosion and a decrease in vegetation potentially accumulating Si, and has led to a twofold to threefold decrease of the base flow delivery of Si18, 19. Due to differences in peat parent material, land use, and peat layer thickness, the Si availability in peatlands is highly variable. Especially in ombrotrophic peatlands, in which mineral weathering and plant growth are mostly decoupled by the peat layer, Si concentrations can be expected to be low in comparison to other ecosystems like forests18. Fen ecosystems connected to local groundwater percolating through mineral matter could be expected to plot in an intermediate range. Most available data for Si, however, is based on bulk concentrations derived from X-ray fluorescence analyses (XRF), which are unable to distinguish between bioavailable Si and refractory forms like quartz20. Only few studies have analyzed the concentrations of bioavailable Si or pore water Si of peatlands, observing varying concentrations between <1 mg/L and >25 mg/L21, 22. In field and laboratory experiments it was found that plants grown under high Si availability showed a higher decomposition rate14,15,16. This suggests that Si may influence the ecosystem function of peatlands, especially in grass dominated fens with silicon being beneficial for grasses23, by accelerating the peat decomposition. Higher peat decomposition rates could increase GHG production and decrease the binding capacity of the peat for nutrients and other elements. However, so far, the effect of Si availability carbon turnover in peatlands is not well known.
Consequently, the aim of this study was to investigate how Si availability influences the biogeochemistry of peatlands. We hypothesize that increased Si availability (i) increases production of CO2 and CH4 as well as DOC release, (ii) mobilizes nutrients, such as N and P, because of competition for binding sites, and (iii) deteriorates water quality by mobilizing potentially toxic trace elements, such as As, due to changes in redox conditions. To this end, a field experiment was conducted at a minerotrophic fen site in Bavaria, Germany. Pore water samples and dissolved gases were analyzed under low and high Si availabilities to identify the effect of Si on CO2 and CH4 production and nutrient and trace element mobilization.
Influence of Si availability on conductivity, CO2, CH4, and DOC in pore water
One month after Si was added, average Si concentrations were threefold higher in the Si+ treatments than in the controls (Fig. 1A). The two treatments (control and Si+ treatment) resulted in significantly different conductivities and pore water concentrations of DOC (p ≤ 0.001), CH4 (p ≤ 0.001), and CO2 (p = 0.003). With Si addition, DOC concentrations and conductivity exceeded values of the controls by a factor of four one month after Si addition and decreased to about the initial values after three months (Fig. 1B and C). In the controls, DOC and conductivity did not show a temporal pattern, but remained more or less constant. Concentrations of CO2 and CH4 of the first measurement were close to zero, probably, because equilibrium between pore water and gas phase in the gas samplers was not reached, yet. These first values were excluded from further interpretation (Fig. 1D and E). In the following months, high Si availability increased both, CO2 and CH4 concentrations, by an average factor of 1.7. However, the difference between high and low Si availability decreased for CO2 and increased for CH4 over time. Since CO2 concentrations were considerably higher than CH4 concentrations (up to 6-fold), the molar CO2:CH4 ratio in both treatments was always larger than one (Fig. 1F). In August and September, the CO2:CH4 ratio under high Si availability was higher than under low Si availability, whereas in October and November it was lower. In addition, we found minor differences (ΔpH < 1) in pore water pH between both treatments at the end of the experiment. These differences, however, did not exceed previously reported natural variability due to redox dynamics at the study site24, which had only minor effects on element mobilization. Furthermore, no significant correlation between differences in pH and any other investigated parameter was found (data not shown). We therefore deduce that pH is no relevant parameter for explaining the observed differences between high and low Si availability.
Influence of Si availability on the element concentrations in pore water
Concentrations of total dissolved N, P, Fe, Mn, Co, and Zn in pore water were significantly higher under high compared to low Si availability (p ≤ 0.001 each, Fig. 2). Apart from Fe, which peaked two months after Si addition, all elements had highest concentrations immediately after Si addition. The highest increase after Si addition was observed for Co (10-fold), followed by Fe (6-fold), and both N and P (both 5-fold). Note that after the elements had reached a temporary maximum, concentrations decreased faster towards initial values than Si concentrations did. Only Zn did not show a continuous decrease, but remained rather stable at elevated concentrations. Under low Si availability, all element concentrations, except for Zn (p < 0.05, ANOVA), did not significantly differ during the experiment (n.s., ANOVA). Remarkably, Si and P concentrations of individual measurements in pore water were highly correlated (Pearson r = 0.89) (Figs 1, 2).
Total As concentrations were also elevated under high Si availability (Fig. 3A). Due to problems with sample preservation, As speciation could not be measured for the last sampling, i.e., four months after Si addition. However, the last available sample suggested a converging trend in concentrations of the As species of both treatments were, differences were statistically insignificant (n.s., t-test) (Fig. 3B). At the beginning of the experiment, directly after the installation of the pore water samplers, arsenate concentrations were higher and organic As species were less abundant than in the following months. In both treatments, inorganic As species dominated (79–95%). Under high Si availability ~70% more arsenite and ~48% less arsenate occurred compared to low Si availability. Furthermore, one month after the Si addition less organic As species occurred in the Si+ treatments, which was reversed thereafter. Under low Si availability, MMA was the dominant organic As species, whereas under high Si availability, DMA was more abundant. Interestingly, in some cases under high Si availability minor amounts of mono-, di-, and trithioarsenate occurred (<0.8 µg/L). Details about the As species (Table S1), general soil characteristics (Table S2), weather data (Figure S1), pH, and temperature in pore water (Figure S2) can be found in the supporting information.
Silicon controls on carbon turnover
The increased concentrations of CO2, CH4, and DOC in pore water under high Si availability clearly confirm the relevance of Si for the carbon cycle and support hypothesis (i) that increased Si availability increases concentrations of CO2 and CH4 in the peat and leads to a release of DOC into porewater (Fig. 1). Assuming otherwise similar conditions of diffusivity and transport as in the control, higher concentrations of CO2 and CH4 would indicate higher emissions of these gases and thus higher rates of decomposition. Moreover, a higher release of DOC into the porewater constitutes another potentially mobile fraction of carbon and thus increased losses of carbon from the system due to increased Si availability. We are aware that increased concentrations would not necessarily be a proof for increased production rates. However, assuming that diffusivity, peat properties, and water saturation of treatment and control remained similar during out experiment and assuming a steady state of production and diffusive emission, increased concentration levels can be interpreted as a result of higher production of CO2 and CH4 in the peat. Since those three components (CO2, CH4 and DOC) represent products of organic matter decomposition10 Si seems to accelerate decomposition and therefore presumably influences the carbon turnover of such ecosystems. This is in line with the findings of Schaller and Struyf14, who showed a positive correlation of plant litter Si concentration and microbial decomposition rate in a laboratory experiment. The same positive effect of Si on litter decomposition was also proven in a field experiment15. Since in our study plants were not present in the mesocosms, direct plant effects on CO2 formation via rhizosphere respiration could be excluded. Consequently, heterotrophic respiration should be the main source of the surplus of CO2 25. The increase in CO2, CH4 and DOC concentration in porewaters could thus be explained by Si mobilizing elements like P into pore waters, due to competition of Si and P for binding sites as already suggested for As26 (Fig. 2). P availability however, interferes with C turnover, as P is known to compete with C for binding sites27 and thus affects the C turnover by increasing pore water DOC concentrations, making organic carbon more accessible for decomposition. Since the molar CO2:CH4 ratio was always higher than one, conditions in the mesocosms were not strictly methanogenic28, but high Si availability temporarily decreased CO2:CH4 ratios (Fig. 1), confirming the effect of Si addition leading to lower redox conditions. Interestingly, high Si availability increased CO2 production at first. Thereafter, upon depletion of thermodynamically favorable electron acceptors, like nitrate and ferric iron, methanogenesis increased under high Si availability28. Simultaneously, decomposition led to a reductive dissolution of redox labile phases, and mobilized DOC and associated elements24. This release increased the ionic strength in Si+ treatments as shown before11. In summary, high Si availability increased CO2 as well as CH4 production and DOC release in fens and favored the rapid onset of reducing conditions.
Enhanced decomposition can not only increase CO2 and CH4 concentrations in pore water, but can also increase outgassing of these GHGs. The extent of the outgassing depends on production, consumption, and transport of the GHGs in pore water, which were, however, not studied in further detail here. In general, outflow, diffusion, advection, bubble formation, and direct transfer by vascular plants are the possible transport mechanisms of CO2 and CH4 29,30,31. Even though in the present study some of the CH4 was probably reoxidized to CO2 32 , and the fate of CH4 was not studied in detail, three months after Si addition, the CH4 concentrations were high enough to form bubbles (>390 µM equivalent to CH4 partial pressures around 0.2 atm) followed by outgassing33. Hence, an increased GHG production is supposed to increase GHG emissions under elevated Si availability.
Silicon controls on nutrient mobilization
High Si availability considerably increased concentrations of N and P in pore water (Fig. 2), confirming hypothesis (ii) that Si mobilizes nutrients. This higher release of N and P into the pore water may be due to dissolved Si competing with other elements for binding sites at organic matter and mineral surfaces. This process was already suggested by Seyfferth and Fendorf for As26, who determined that Si effectively competes with P for binding sites of Fe and Al(hydr)oxides in rice paddy fields and Neu et al. showing a higher P uptake by plants under high Si availability suggesting elevated P mobility23. The fact that Si highly correlated with P in our experiment strongly supports that P is displaced by Si at the binding sites. From previous studies it is known that at this particular fen site, but likely also in other fens, iron oxides are present in notable quantities34. Additional to the competition at binding sites, Si also apparently accelerated the mineralization of organic matter, as obvious from increases in CO2 and CH4, causing additional N and P release from decomposition into the pore water35. The fact that N and P concentrations decreased towards initial concentrations within a few months after the Si application may have partly resulted from increased microbial assimilation of nutrients36. However, leaching might also have been effective due to high runoff rates after intensive precipitation events at the study site9 (Figure S1). Most probably some of the Si also leached out, but the dissolution of Si from the Si-nanoparticle addition (amorphous Si), which remained in suspension, may have buffered the Si leaching37.
Besides the macronutrients N and P, also the concentrations of micronutrients and potentially toxic elements such as Fe, Mn, Co, Zn, and As increased in pore water after Si addition (Fig. 3), confirming hypothesis (iii) that increased Si availability can deteriorate water quality by mobilizing potentially toxic trace elements. Similar to N and P, these elements may have been replaced by Si at the binding sites, and as a result the re-adsorption may be controlled by the competition between these elements26. Additionally, Fe, Mn, Co, and As are redox-sensitive, as already mentioned above. Therefore, their release might be driven by the more rapid and stronger decrease in redox conditions towards methanogenesis under high Si availability. Consequently, elements bound to ferric iron phases and DOC co-precipitated with iron were released into solution11, 38. In comparison to the other studied elements, the decrease of Fe was delayed hinting to ongoing reductive dissolution of a large pool of iron phases under continuously reduced conditions39. Moreover, the dissolution of Fe and Mn (hydro)oxides may have released bound As and P into solution40. Due to the high activity of sulfate reduction and subsequent formation of sulfides at the site the potential of As and P re-adsorption is low and the elements remain in solution41. The shift towards reducing conditions was also reflected in the increase of the reduced As species arsenite under high Si availability (Fig. 3). Furthermore, the continuous methylation from MMA to DMA under high Si availability conditions occurred simultaneously to the increased activity of methanogens, which are able to methylate As42. Lafferty and Loeppert43 showed a decrease of As adsorption at Fe (hydr)oxide surfaces by increased methyl substitution of As. This may explain the increased As release under high Si availability. Nevertheless, high Si availability did not only increase total As concentrations but also released higher concentrations of the more toxic and mobile arsenite into the pore water44. These observations also demonstrate that the analysis of As speciation provided additional insight into the particular biogeochemical conditions, i.e., with Si favoring strongly reducing conditions with concomitant sulfate reduction and methanogenesis.
In conclusion, Si availability among different peatlands is highly different and thus may indeed exert an important control on biogeochemical processes as observed in our study. Our results revealed that high Si availability leads to N and P mobilization (Fig. 4). However, high Si availability in fens is potentially accelerating organic matter decomposition by increasing CO2 and especially CH4 production (Fig. 4). Competing with other elements, such as N, P, and As, for binding sites and promoting reducing conditions, high Si availability causes an enhanced nutrient and toxicant mobilization into solution (Fig. 4). In summary, Si exerts an important and so far little considered control on carbon and nutrient turnover in peatlands.
The Si addition experiment was conducted at the “Schlöppnerbrunnen fen site”, which is located in the Lehstenbach catchment (4.2 km2) in northeastern Bavaria (50°07′56.8″ N, 11°52′55.5″ E, 697 m ASL). The study area is characterized by a continental temperate climate with a mean annual precipitation of 1038 mm (1994–2015) and mean annual temperature of 6.5 °C (1994–2015). The minerotrophic peatland has a central open area, which is surrounded by a spruce forest (Picea abies). The open area is covered by grasses and bryophytes45. The thickness of the peat varies from 30 to 120 cm and the organic material is highly decomposed below 5–10 cm depth46. The site has been intensively studied previously with regard to carbon turnover and redox processes (e.g. refs 34, 47), carbon export24, CO2 exchange48, and microbiology (e.g. ref. 49). During the experiment, the peat was waterlogged and the water tables were constantly 2 cm above the soil surface.
The experiment was set up in July 2015. Eight mesocosms (PVC tubes, length: 40 cm, diameter: 15 cm) were installed in pairs (each containing one control and one treatment with Si addition about 10 cm apart from each other) randomly selected within homogeneous but sparse vegetation of Carex rostrata. However, those plants died due to installation of the tubes before the start of the experiment. Soil samples from the top peat layer were taken close to the pairs of mesocosms. Each pair consisted of a mesocosm to which 50 g of low acidic (pH ~ 4.7), synthetic amorphous silica (Aerosil 300; Evonik Industries AG) was added, which is equal to 23 g of elemental Si, suspended in 1 L deionized water (Si+ treatment) and a control watered with 1 L deionized water. The synthetic amorphous silica is highly dissolvable even at lower pH and is an analogue to nano particulate volcanic ash. The Si was added only once at the beginning of the experiment.
Pore water and gas samples were obtained from each mesocosm on a monthly basis between July and November, with the first sampling starting directly before Si addition to the Si treatment. To this end, gas samplers (polysiloxane tubes allowing diffusive equilibration, length: 20 cm, diameter: 1 cm) were installed in the top peat layer just below the surface. The polysiloxane tubes were connected to impermeable polyurethane tubes and closed by three-way stopcocks for sampling following a sampling procedure described previously50. The gas samples were taken with plastic syringes (3 ml, Omnifix, Braun). Pore water was sampled by polyvinylchloride tubes (length: 50 cm, diameter: 1 cm), which were installed in the top peat layer of each mesocosm. The lowest 15 cm of the tubes, which were placed in the top peat layer just below the surface, were perforated at 5 mm intervals. To eliminate coarse particles, the tubes were covered by nylon prefilters. The pore water samples were taken by syringes to measure pH, temperature (HQ40d Multi, PHC101 electrode, Hach), and conductivity (Winlab, Windaus). For the analysis of total element and species concentrations, all water samples were filtered after extraction (0.2 µm, cellulose-acetate filter, Chromafil). To prevent precipitation of sulfide or iron minerals, samples for total element analysis 150 µL of H2O2 (analytical grade, 30%, Fisher Scientific) and 250 µL HNO3 (analytical grade, 70%, Fisher Scientific) were added per 10 mL sample. Samples used for total N and DOC measurement were frozen after the sampling. Samples for species analysis of As were preserved by immediate flash-freezing in dry ice on site.
Concentrations of CO2 and CH4 in the gas samples, which were obtained from the diffusive equilibration samplers, were measured within three hours after the sampling by a gas chromatograph (SRI 8610 C equipped with methanizer and flame ionization detector, SRI Instruments). Concentrations of the dissolved gas in the pore water were recalculated applying the Henry’s law and a temperature correction for in-situ temperatures51. The soil samples were freeze-dried and then ground to fine powder with a zirconium ball mill. Subsequently soil samples were digested in 3 ml of HNO3 and 2 ml H2O2 using a CEM Mars5 microwave digestion system (CEM Corporation, Matthews, NC, USA). An ICP-OES (Vista – Pro, Varian Inc./ Cetac) was used to measure total Si concentrations in the pore water samples and in the soil samples after extraction in a 0.1 M Na2CO3 solution at 85 °C for five hours according to Struyf, et al.52. Total dissolved element concentrations in pore water and soil extracts of P, manganese (Mn), iron (Fe), cobalt (Co), zinc (Zn), and As were analyzed by a quadrupole ICP-MS (X-Series2, Thermo Scientific). The elements Mn, Fe, Co, and Zn were measured in KED mode (kinetic energy discrimination, 3 V energy discrimination for Fe und 2 V for Mn, Co, and Zn, 93% helium and 7% hydrogen as collision gas), whereas P and As were measured in oxygen mode (10% oxygen in helium as a reaction gas). Total element concentrations of N and DOC concentrations in pore water were measured with a TOC-Analyzer (TOC-V CPN, Shimadzu Corporation) with an integrated total N measuring unit. The As speciation analysis was done using an ion chromatography (IC Dionex 3000 with a AG16/AS16 on Ion-Pac column, 4 mm, Thermo Scientific) coupled to a quadrupole ICP-MS (X-Series2, Thermo Scientific). An alkaline eluent with a gradient of 2.5–100 mM NaOH was used at a constant flow rate of 1.2 mL/min as described before53. For quantification, commercially available salts of arsenite, arsenate, monomethylarsenate (MMA), and dimethylarsenate (DMA) were used. Mono-, di- and trithioarsenate were quantified via the arsenate calibration. The validity of this approach has been confirmed before53.
A t-test and a multifactorial analysis of variance (ANOVA) of the factors treatment (high and low Si availability) and time (month) were applied using SPSS version 16.01.
Yu, Z., Loisel, J., Brosseau, D. P., Beilman, D. W. & Hunt, S. J. Global peatland dynamics since the Last Glacial Maximum. Geophys. Res. Lett. 37 (2010).
Settele, J. et al. In Climate Change 2014: Impacts, Adaptation, and Vulnerability. Part A: Global and Sectoral Aspects. Contribution of Working Group II to he Fifth Assessment Report of the Intergovernmental Panel on Climate Change (Cambridge University Press, 2015).
Smith, L. et al. Siberian peatlands a net carbon sink and global methane source since the early Holocene. Science 303, 353–356 (2004).
Roulet, N. T. Peatlands, carbon storage, greenhouse gases, and the Kyoto Protocol: Prospects and significance for Canada. Wetlands 20, 605–615, doi:10.1672/0277-5212 (2000).
Kimmel, K. & Mander, Ü. Ecosystem services of peatlands: Implications for restoration. Prog. Phys. Geogr. 34, 491–514, doi:10.1177/0309133310365595 (2010).
Martin-Ortega, J., Allott, T. E., Glenk, K. & Schaafsma, M. Valuing water quality improvements from peatland restoration: Evidence and challenges. Ecosyst. Serv. 9, 34–43 (2014).
Daniels, S., Evans, M., Agnew, C. & Allott, T. Sulphur leaching from headwater catchments in an eroded peatland, South Pennines, UK. Sci. Total Environ. 407, 481–496 (2008).
Rothwell, J. J. et al. Storage and behavior of As, Sb, Pb, and Cu in ombrotrophic peat bogs under contrasting water table conditions. Environ. Sci. Technol. 44, 8497–8502 (2010).
Strohmeier, S. et al. Concentrations and fluxes of dissolved organic carbon in runoff from a forested catchment: insights from high frequency measurements. Biogeosciences 10, 905–916, doi:10.5194/bg-10-905-2013 (2013).
Limpens, J. et al. Peatlands and the carbon cycle: from local processes to global implications - a synthesis. Biogeosciences 5, 1475–1491 (2008).
Knorr, K. H. DOC-dynamics in a small headwater catchment as driven by redox fluctuations and hydrological flow paths - are DOC exports mediated by iron reduction/oxidation cycles? Biogeosciences 10, 891–904, doi:10.5194/bg-10-891-2013 (2013).
Mestrot, A., Planer-Friedrich, B. & Feldmann, J. Biovolatilisation: a poorly studied pathway of the arsenic biogeochemical cycle. Environmental Science-Processes & Impacts 15, 1639–1651, doi:10.1039/c3em00105a (2013).
Bridgham, S. D., Updegraff, K. & Pastor, J. Carbon, nitrogen, and phosphorus mineralization in northern wetlands. Ecology 79, 1545–1561 (1998).
Schaller, J. & Struyf, E. Silicon controls microbial decay and nutrient release of grass litter during aquatic decomposition. Hydrobiologia 709, 201–212, doi:10.1007/s10750-013-1449-1 (2013).
Schaller, J., Hines, J., Brackhage, C., Baucker, E. & Gessner, M. O. Silica decouples fungal growth and litter decomposition without changing responses to climate warming and N enrichment. Ecology 95, 3181–3189 (2014).
Marxen, A. et al. Interaction between silicon cycling and straw decomposition in a silicon deficient rice production system. Plant Soil 398, 153–163, doi:10.1007/s11104-015-2645-8 (2016).
Struyf, E. & Conley, D. J. Emerging understanding of the ecosystem silica filter. Biogeochemistry 107, 9–18, doi:10.1007/s10533-011-9590-2 (2012).
Struyf, E. et al. Historical land use change has lowered terrestrial silica mobilization. Nat. Commun. 1, 129 (2010). doi:12910.1038/ncomms1128.
Conley, D. J. et al. Deforestation causes increased dissolved silicate losses in the Hubbard Brook Experimental Forest. Glob. Chang. Biol. 14, 2548–2554, doi:10.1111/j.1365-2486.2008.01667.x (2008).
Biester, H., Hermanns, Y.-M. & Cortizas, A. M. The influence of organic matter decay on the distribution of major and trace elements in ombrotrophic mires–a case study from the Harz Mountains. Geochim. Cosmochim. Acta 84, 126–136 (2012).
Bennett, P. C., Siegel, D. I., Hill, B. M. & Glaser, P. H. Fate of silicate minerals in a peat bog. Geology 19, 328–331 (1991).
Sommer, M., Kaczorek, D., Kuzyakov, Y. & Breuer, J. Silicon pools and fluxes in soils and landscapes - a review. J. Plant Nutr. Soil Sci. 169, 310–329, doi:10.1002/jpln.200521981 (2006).
Neu, S., Schaller, J. & Dudel, E. G. Silicon availability modifies nutrient use efficiency and content, C:N:P stoichiometry, and productivity of winter wheat (Triticum aestivum L.). Scientific Reports 7, 40829, doi:10.1038/srep40829 (2017).
Knorr, K.-H. DOC-dynamics in a small headwater catchment as driven by redox fluctuations and hydrological flow paths–are DOC exports mediated by iron reduction/oxidation cycles? Biogeosciences 10, 891–904 (2013).
Hanson, P. J., Edwards, N. T., Garten, C. T. & Andrews, J. A. Separating root and soil microbial contributions to soil respiration: A review of methods and observations. Biogeochemistry 48, 115–146, doi:10.1023/A:1006244819642 (2000).
Seyfferth, A. L. & Fendorf, S. Silicate mineral impacts on the uptake and storage of arsenic and plant nutrients in rice (Oryza sativa L.). Environ. Sci. Technol. 46, 13176–13183 (2012).
Schneider, M. P. W. et al. Sorptive stabilization of organic matter by amorphous Al hydroxide. Geochim. Cosmochim. Acta 74, 1606–1619, doi:10.1016/j.gca.2009.12.017 (2010).
Yavitt, J. B. & Seidmann-Zager, M. Methanogenic conditions in northern peat soils. Geomicrobiol J 23, 119–127, doi:10.1080/01490450500533957 (2006).
Walter, B. P. & Heimann, M. A process-based, climate-sensitive model to derive methane emissions from natural wetlands: Application to five wetland sites, sensitivity to model parameters, and climate. Glob. Biogeochem. Cycles 14, 745–765, doi:10.1029/1999gb001204 (2000).
Laanbroek, H. J. Methane emission from natural wetlands: interplay between emergent macrophytes and soil microbial processes. A mini-review. Ann. Bot. 105, 141–153, doi:10.1093/aob/mcp201 (2010).
Le Mer, J. & Roger, P. Production, oxidation, emission and consumption of methane by soils: A review. Eur. J. Soil Biol. 37, 25–50, doi:10.1016/S1164-5563(01)01067-6 (2001).
Segarra, K. E. A. et al. High rates of anaerobic methane oxidation in freshwater wetlands reduce potential atmospheric methane emissions. Nat. Commun. 6, doi:10.1038/Ncomms8477 (2015).
Beer, J. & Blodau, C. Transport and thermodynamics constrain belowground carbon turnover in a northern peatland. Geochim. Cosmochim. Ac. 71, 2989–3002, doi:10.1016/j.gca.2007.03.010 (2007).
Knorr, K.-H., Lischeid, G. & Blodau, C. Dynamics of redox processes in a minerotrophic fen exposed to a water table manipulation. Geoderma 153, 379–392 (2009).
Mettrop, I. S., Cusell, C., Kooijman, A. M. & Lamers, L. P. M. Nutrient and carbon dynamics in peat from rich fens and Sphagnum-fens during different gradations of drought. Soil Biol. Biochem. 68, 317–328, doi:10.1016/j.soilbio.2013.10.023 (2014).
Lin, X. et al. Microbial Metabolic Potential for Carbon Degradation and Nutrient (Nitrogen and Phosphorus) Acquisition in an Ombrotrophic Peatland. Appl. Environ. Microb. 80, 3531–3540, doi:10.1128/Aem.00206-14 (2014).
Aureli, F. et al. Investigating agglomeration and dissolution of silica nanoparticles in aqueous suspensions by dynamic reaction cell inductively coupled plasma-mass spectrometry in time resolved mode. J. Anal. At. Spectrom. 27, 1540–1548, doi:10.1039/c2ja30133d (2012).
Olivie-Lauquet, G. et al. Release of trace elements in wetlands: Role of seasonal variability. Water Res 35, 943–952, doi:10.1016/S0043-1354(00)00328-6 (2001).
Knorr, K. H., Lischeid, G. & Blodau, C. Dynamics of redox processes in a minerotrophic fen exposed to a water table manipulation. Geoderma 153, 379–392, doi:10.1016/j.geoderma.2009.08.023 (2009).
ThomasArrigo, L. K. et al. Iron and Arsenic Speciation and Distribution in Organic Flocs from Streambeds of an Arsenic-Enriched Peat land. Environ. Sci. Technol. 48, 13218–13228, doi:10.1021/es503550g (2014).
Blodau, C., Fulda, B., Bauer, M. & Knorr, K. H. Arsenic speciation and turnover in intact organic soil mesocosms during experimental drought and rewetting. Geochim. Cosmochim. Ac. 72, 3991–4007, doi:10.1016/j.gca.2008.04.040 (2008).
Bentley, R. & Chasteen, T. G. Microbial methylation of metalloids: Arsenic, antimony, and bismuth. Microbiol. Mol. Biol. Rev. 66, 250–271, doi:10.1128/Mmbr.66.2.250-271.2002 (2002).
Lafferty, B. J. & Loeppert, R. H. Methyl arsenic adsorption and desorption behavior on iron oxides. Environ. Sci. Technol. 39, 2120–2127, doi:10.1021/es048701+ (2005).
Jain, C. K. & Ali, I. Arsenic: Occurrence, toxicity and speciation techniques. Water Res. 34, 4304–4312, doi:10.1016/s0043-1354(00)00182-2 (2000).
Gerstberger, P. Die BITÖK-Untersuchungsflächen im Fichtelgebirge und Steigerwald (BITÖK, 2001).
Paul, S., Kusel, K. & Alewell, C. Reduction processes in forest wetlands: Tracking down heterogeneity of source/sink functions with a combination of methods. Soil Biol. Biochem. 38, 1028–1039, doi:10.1016/j.soilbio.2005.09.001 (2006).
Alewell, C., Paul, S., Lischeid, G., Küsel, K. & Gehre, M. Characterizing the redox status in three different forested wetlands with geochemical data. Environ. Sci. Technol. 40, 7609–7615 (2006).
Otieno, D. et al. Responses of CO2 exchange and primary production of the ecosystem components to environmental changes in a mountain peatland. Ecosystems 12, 590–603 (2009).
Reiche, M., Torburg, G. & Küsel, K. Competition of Fe (III) reduction and methanogenesis in an acidic fen. FEMS microbiology ecology 65, 88–101 (2008).
Knorr, K.-H. & Blodau, C. Impact of experimental drought and rewetting on redox transformations and methanogenesis in mesocosms of a northern fen soil. Soil Biology and Biochemistry 41, 1187–1198, doi:10.1016/j.soilbio.2009.02.030 (2009).
Lide, D. & Frederikse, H. CRC handbook of chemistry and physics. 74th edn CRC Press. Boca Raton (1993).
Struyf, E., Morth, C. M., Humborg, C. & Conley, D. J. An enormous amorphous silica stock in boreal wetlands. J. Geophys. Res.: Biogeosci. 115, doi:10.1029/2010jg001324 (2010).
Planer-Friedrich, B., London, J., McCleskey, R. B., Nordstrom, D. K. & Wallschläger, D. Thioarsenates in geothermal waters of Yellowstone National Park: determination, preservation, and geochemical importance. Environ. Sci. Technol. 41, 5245–5251 (2007).
We are grateful to Dr. Ben Gilfedder (Hydrology Department at the University of Bayreuth) for providing field and laboratory equipment. Sincere thanks also to Dr. Wolfgang Babel (Micrometeorology Department at the University of Bayreuth), who gave us access to the weather data. For help in the laboratory, we thank Stefan Will, Silke Hammer, Heidi Zier, Margarete Wartinger, as well as Martina Rohr, who additionally measured the N and DOC concentrations. Moreover, we are especially grateful to Andreas Kolb and Johannes Olesch for borrowing, calibrating, and installing the weather station. This publication was funded by the German Research Foundation (DFG) and the University of Bayreuth in the funding programme Open Access Publishing.
The authors declare that they have no competing interests.
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Reithmaier, GM.S., Knorr, KH., Arnhold, S. et al. Enhanced silicon availability leads to increased methane production, nutrient and toxicant mobility in peatlands. Sci Rep 7, 8728 (2017). https://doi.org/10.1038/s41598-017-09130-3
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