Cytochrome c oxidase catalyses the reduction of molecular oxygen to water while the energy released in this process is used to pump protons across a biological membrane. Although an extremely well-studied biological system, the molecular mechanism of proton pumping by cytochrome c oxidase is still not understood. Here we report a method to produce large quantities of highly diffracting microcrystals of ba 3-type cytochrome c oxidase from Thermus thermophilus suitable for serial femtosecond crystallography. The room-temperature structure of cytochrome c oxidase is solved to 2.3 Å resolution from data collected at an X-ray Free Electron Laser. We find overall agreement with earlier X-ray structures solved from diffraction data collected at cryogenic temperature. Previous structures solved from synchrotron radiation data, however, have shown conflicting results regarding the identity of the active-site ligand. Our room-temperature structure, which is free from the effects of radiation damage, reveals that a single-oxygen species in the form of a water molecule or hydroxide ion is bound in the active site. Structural differences between the ba 3-type and aa 3-type cytochrome c oxidases around the proton-loading site are also described.
Cytochrome c oxidases (CcOs) are the terminal enzymes of the respiratory chains in mitochondria and many bacteria. They are integral membrane bound complexes that catalyse the reduction of molecular oxygen to water and utilize the energy released to translocate protons across the cell membrane. The free energy stored in this electrochemical proton gradient is used by the cell to synthesize ATP and to drive transmembrane transport. The CcOs have been intensively studied over the last six decades. A vast body of spectroscopic work has detailed the time course of electron and proton movements within the enzymes. Cytochrome c delivers electrons from the positive side of the membrane at the same time as protons are taken up from the negative side of the membrane to the heme a 3 - CuB active site, where the reaction with oxygen takes place. This creates a charge separation across the membrane which is further enhanced by the additional pumping of protons from the positive to the negative side of the membrane (see ref. 1 for a review).
In the aa 3-type CcOs, including the mitochondrial enzyme, two functional proton pathways have been localized; the D- and the K-pathway. It is generally understood that the four protons to be pumped, as well as two substrate protons to be used in the chemical reaction, are taken up through the D-pathway and the remaining two substrate protons are taken up through the K-pathway, for each complete reaction cycle2,3,4,5. In the ba 3-type CcO from the thermophilic bacterium Thermus thermophilus (ba 3 CcO), a single proton pathway is used to transport all four substrate protons from the cytoplasmic (negative) side of the membrane to the active site, as well as the two protons to be translocated all the way across to the periplasmic (positive) side6, 7. Thus, the ba 3 CcO pumps protons with a lower stoichiometry compared to the aa 3-type CcOs, with only two (as opposed to four) protons pumped per reaction cycle7. Despite these differences, the general belief is that all members of the CcO superfamily utilize a common mechanism for proton pumping. The key question is; what is the structural mechanism linking oxygen reduction to proton pumping?
Many members of the CcO superfamily have been structurally characterized in detail8,9,10,11,12. In addition to the oxidized resting state of the enzyme there are structures available also of the reduced form with and without bound ligand13, 14, CO-bound form before and after flash-photolysis15 and different mutant forms of the enzyme9, 16, 17. All previous structures, however, have been solved using either frozen crystals, or at room temperature but under conditions likely to cause radiation damage18, 19. In addition, there are no high-resolutions structures of any of the intermediate states that occur during the reaction cycle and consequently there is no detailed structural understanding of how unidirectional proton translocation takes place.
X-ray Free Electron Lasers (XFELs) offer the possibility to collect diffraction data using extremely intense X-ray pulses of femtoseconds in duration, enabling diffraction to occur before the onset of radiation damage20. This avoidance of radiation damage is of particular importance when studying proteins that contain metal cofactors such as the CcOs21, 22. With the introduction of serial femtosecond crystallography (SFX)23, it has become possible to collect diffraction data on a continuous stream of micrometer sized crystals that flow across a focused XFEL beam. Each microcrystal gives rise to one diffraction image that is then merged with data collected from other microcrystals to recover a complete diffraction data set. Here we present the first room-temperature structure of a CcO determined by SFX. This opens up for future possibilities to track structural changes as oxygen is reduced to water and protons are pumped across the membrane in real time.
Crystallization, characterization, LCP injection and data collection
Conditions known to produce lipidic cubic phase (LCP) crystals of ba 3 CcO from Thermus thermophilus sufficiently large for synchrotron based crystallography24 were modified to yield showers of micrometer sized crystals. A protocol was then developed to prepare microcrystals in glass plates or glass syringes by surrounding the protein-lipid mixture with precipitant solution, similar to a method previously described25. Under these conditions a large number of well-diffracting 5–20 µm sized ba 3 CcO crystals appeared after 2–3 days (Fig. 1).
The oxidation state of ba 3 CcO after crystallization was checked by recording an optical absorbance spectrum from microcrystals as prepared in the LCP phase (Supplementary Figure S1). The peak at 415 nm shows that the enzyme is in the oxidised state within the microcrystals. A spectrum was also recorded of microcrystals reduced by the addition of sodium dithionite (Supplementary Figure S1). This caused a shift of the absorption peak to 427 nm with a shoulder at 445 nm, and the appearance of peaks at 528 nm and 560 nm, characteristic of the reduced enzyme.
X-ray diffraction data were collected at SPring-8 Angstrom Compact Free Electron Laser (SACLA) with highly brilliant X-ray pulses at 7.6 keV and approximately 10 fs in duration. Microcrystals were delivered into the X-ray focus at a flow rate of 0.48 µl/min using an LCP injector developed at SACLA26. A total of 87,057 frames were collected out of which 11,374 were identified as containing a diffraction pattern using the software Cheetah27, of which 8,211 frames were indexed and merged using CrystFEL28. Thus a complete diffraction data set was collected from approximately 50 minutes of data collection using 25 µl of sample. The structure was solved by molecular replacement using Phaser29 with PDB code 3S8F as a search model, and refined to a resolution of 2.3 Å. In the following text we refer to the room-temperature structure described here as the SFX structure (Fig. 2a).
Room-temperature ba 3 CcO structure
A number of structures of ba 3 CcO are available in the protein data bank (PDB), both using vapor diffusion and LCP crystallization methods11, 17, 24. The highest resolution structures at 1.8 Å resolution were obtained by LCP crystallization (PDB codes 3S8F and 3S8G for wild type and A120F mutant enzyme, respectively)24. All previously reported structures, however, have been determined using crystals cooled to cryogenic temperatures (~100 K). In the following discussion we use the PDB code 3S8G structure for comparison (referred to as the cryo-LCP structure) as it has the best defined electron density.
Overall, the SFX structure (Fig. 2a) agrees very well with previously solved structures, with a Cα root mean-square deviation of 0.32 Å compared to the cryo-LCP structure. The space group, C2, is the same as the cryo-LCP structure with similar unit cell parameters. We can confirm the presence of 13 out of 20 lipid molecules modelled in the cryo-LCP structure, and we also localize two new lipid tails associated with subunit I (Supplementary Figure S2). In the SFX structure we resolve 92 water molecules, which compares with 225 observed in the cryo-LCP structure. This difference in number of modelled water molecules is possibly due to the lower resolution of the SFX structure, or because of increased disorder of water molecules when diffraction data are recorded at room temperature.
Active site ligand
The active site of CcOs is made up of heme a 3, CuB and surrounding residues. This site is where molecular oxygen binds in to the reduced heme a 3 iron and the O=O double bond is cleaved through the uptake of four electrons and four protons, producing two water molecules. In several structures of oxidized CcO the electron density at the active site has been interpreted to indicate the presence of peroxide (O2 2−) bound between the heme a 3 iron and CuB 24, 30, 31. This interpretation has been questioned by Kaila and co-workers who claim that a stable peroxide in the active site of oxidized CcO is unlikely32. Their arguments were based upon quantum chemical calculations in combination with spectroscopic data and re-refinement of two X-ray structures. For the ba 3 CcO, as many as 34 structures have been deposited in the PDB from crystals grown using the method of vapor diffusion11 and from lipidic cubic phase crystallization24. These structures are summarized in Supplementary Table S1. Structures resulting from vapor diffusion crystallization show conflicting results regarding the ligand bound in the active site, where the omit map electron density appears spherical in some cases (best fitted with a single-oxygen species such as water) and elongated in other cases (best fitted with a peroxide species) (Supplementary Figure S3). The authors attribute this uncertainty to effects of temperature as well as time of exposure to X-rays24, 33. All fourteen structures of ba 3 CcO resulting from LCP crystallization display somewhat elongated omit map electron density in the active site, which has repeatedly been interpreted to indicate a bound peroxide molecule (Supplementary Table S1). However, this elongated density is potentially due to X-ray induced reduction24.
Our SFX structure displays residual electron density for a ligand in the active site. Since this residual density in the unbiased Fo-Fc omit map is spherical in shape it strongly suggests that a single-oxygen species, presumably a water molecule or hydroxide ion, is bound between the heme a 3 iron and CuB (Figs 3 and 4a). Moreover, the peak height of this active site feature (0.99 e−/Å3) is very similar to that of a strongly bound water molecule located between Ser309 and the heme a 3 secondary alcohol (0.86 e−/Å3), further supporting our interpretation that the active site residual density corresponds to a single tightly bound water molecule or hydroxide ion. In contrast, a peroxide molecule was modelled as the active site ligand in the cryo-LCP structure24, and the Fo-Fc omit map calculated from the deposited cryo-LCP data clearly displays an elongated electron density peak in the active site (Fig. 4b). When a water molecule is modelled into the active site of the SFX structure, some residual positive Fo-Fc electron density remains (Fig. 4d). However, if a water molecule is modelled into the cryo-LCP structure in place of the peroxide, it results in a poor fit with a much stronger residual density (Fig. 4e). Similarly, the structure of bovine heart aa 3-type CcO derived from XFEL diffraction data using large cryo-protected crystals (PDB code 3WG7)31 also shows an elongated shape of the active site peak in the Fo-Fc omit map (Fig. 4c). Moreover, if a single water molecule is built into this electron density in place of the peroxide ligand, the fit is poor with a strong residual Fo-Fc density similar to in the cryo-LCP structure (Fig. 4f).
Resonance Raman spectroscopy has shown the existence of a peroxide species bridging the heme a 3 iron and CuB in the so-called “resting oxidized state” of aa 3-type CcO from bovine heart34. This has not yet been explicitly investigated for the ba 3 CcO, but electrochemically induced UV-vis difference absorbance spectra suggest that there is no peroxide molecule at the active site of ba 3 CcO in the oxidized (as prepared) state of the enzyme35. On the other hand, it has been demonstrated that the redox sites of ba 3 CcO crystals become reduced during X-ray data collection at cryogenic temperatures33. Thus the apparent peroxide species in the cryo-LCP structures (Supplementary Table S1) may result from the recombination of two hydroxyl radicals produced from X-ray damage to water molecules near the active site, as the authors also suggest24. In contrast, our SFX structure represents a radiation-damage free structure of ba 3 CcO for which a water molecule or hydroxide ion is likely the active site ligand.
Proton translocation pathway in ba 3 CcO
The ba 3 CcO utilizes a single proton uptake pathway, the so-called K-pathway analogue, in order to provide protons for the oxygen chemistry at the active site, as well as to transport protons that are pumped across the membrane. In the SFX structure we can track a proton pathway that stretches from Glu15II (“II” denotes subunit II), via Thr315, Wat721, Tyr248, Thr312, Tyr244, Ser309, Wat738 and the secondary alcohol of heme a 3 to Tyr237 in close proximity of the active site (Fig. 2b). The positions of the two water molecules as well as the side chain conformations of key residues are conserved when comparing with the cryo-LCP structure24. Most importantly, the room-temperature SFX structure confirms that there is a gap in the hydrogen bond network between Thr312 and Tyr244, with a distance of 4.45 Å between the two residues. It is possible that transient ordering of a water molecule bridges this gap such that a functional proton pathway is formed when a proton is taken up from the negative side of the membrane.
Structural differences around the proton-loading site
Transmembrane proton pumping against an electrochemical gradient requires a protonatable group with alternating access to the positive and negative sides of the membrane during the catalytic cycle. There is an ongoing debate as to the identity of this group, referred to as the proton-loading site, where it has been suggested to be localized in the vicinity of the heme a 3 propionates; the surrounding water cluster; or a near-by aspartic acid residue (Asp372 in ba 3 CcO)36, 37. Several of the residues in this region are conserved throughout the CcO superfamily including a tryptophan residue (Trp229 in ba 3 CcO). It has been shown that in the aa 3-type CcO from Paracoccus denitrificans the corresponding residue forms a tryptophan radical during enzymatic turnover38. This, however, is not the case for ba 3 CcO. It was recently proposed that the transient formation of the tryptophan radical is involved in proton pumping, and consequently the lack of such a radical in ba 3 CcO could be related to the lower proton pumping stoichiometry displayed by this enzyme39. In light of this debate, it is interesting to note that the SFX structure and all previously published ba 3 CcO structures differ from the aa 3-type CcO structures in the vicinity of this conserved tryptophan residue (Fig. 2c). Specifically, the tyrosine residue (Tyr136 in ba 3 CcO) that hydrogen bonds to the tryptophan is in close proximity of the heme a 3 propionates in ba 3 CcO, whereas it is pointing more towards the associated water cluster in the aa 3-type CcOs. It may also be of relevance for the difference in proton pumping efficiency that all aa 3-type CcOs contain a magnesium ion as part of the above mentioned water cluster. There is no magnesium ion present in our SFX structure or any of the previously deposited ba 3 CcO structures. Instead, the position of the magnesium ion is occupied by the carboxyl group of a glutamic acid residue (Glu126II) in the ba 3 CcO structures (Fig. 2c). Calculations have shown that the magnesium ion affects the proton affinity of the heme a 3 propionate A in the aa 3-type CcOs40.
We describe a method to prepare large quantities of well-diffracting microcrystals of ba 3 CcO in lipidic phase and present the first room-temperature structure of a CcO free of radiation damage. The omit electron density map shows that the ligand bound at the active site in the oxidized form of ba 3 CcO is most likely a single-oxygen species corresponding to a water molecule or hydroxide ion bound between the heme a 3 iron and CuB. Considering the fact that it is localized between two positively charged groups, the heme a 3 iron and CuB, a hydroxide ion may be the more likely alternative. Our conclusion is based upon the spherical shape of the electron density peak in the unbiased Fo-Fc omit map and comparison of residual densities when modelling a single water molecule at this site in other deposited CcO structures (Fig. 4). It is also apparent that the proton transfer pathway and the water cluster associated with the presumed proton-loading site, described in previous cryo-temperature structures11, 24, are conserved at room temperature.
Hirata and co-workers recently presented a structure of bovine heart aa 3-type CcO from XFEL diffraction data collected using large cryo-protected crystals where the crystals were physically translated between each X-ray exposure31. We propose that the use of microcrystals in order to collect room-temperature SFX data has major advantages over the use of large, cryo-cooled crystals at an XFEL. Specifically, it is clearly beneficial to determine the structure of a protein at room temperature, as this is the more physiologically relevant temperature. Further, each microcrystal is only exposed once to an XFEL pulse when using SFX. This completely avoids the risk of radiation damage accumulating due to X-ray induced free radicals propagating through the crystal. In this study an average X-ray dose of 13 MGy per crystal was used, well below the limit where radiation damage has been detected in metal clusters using XFEL radiation22. Microcrystals are also favoured over large crystals if the aim is to trigger a reaction within the crystal. For microcrystals the penetration of the reaction trigger, may it be light or a chemical, is more likely to reach and activate a large population of the protein molecules within the crystal. This idea has been very successfully demonstrated in several recent examples26, 41, 42. It is therefore exciting to consider future opportunities for time-resolved structural studies now that we have demonstrated the possibility to collect high resolution SFX data on CcO crystals. This opens up a unique opportunity to record structural snapshots of CcO as oxygen is reduced to water while protons are simultaneously pumped across a biological membrane.
Expression and purification
Recombinant ba 3 CcO from Thermus thermophilus was produced and purified as described43 with the modifications described below. The cell pellet was resuspended in lysis buffer (50 mM Hepes pH 8.0, 100 mM NaCl, 1 spatula tip of DNase, 2 spatula tips of PMSF) and sonicated. Unbroken cells were collected and sonicated a second time. Membrane proteins were extracted from the membrane twice in extraction buffer (50 mM Hepes pH 8.0, 2.5% Triton X-100); first at 4 °C overnight and then for 4 h at room temperature. Solubilized ba 3 CcO was bound to 2 × 5 ml prepacked Ni-NTA columns (HisTrap HP, GE Healthcare Life Science) equilibrated with buffer (10 mM Hepes pH 8.0, 150 mM NaCl, 1% Triton X-100, 10 mM imidazole). The protein was then washed and eluted with equilibration buffer containing 25 mM and 250 mM imidazole, respectively. Pooled fractions were dialyzed against 5 mM Hepes pH 8.0, 0.05% Triton X-100 at 4 °C for 4 h and then against 5 mM Hepes pH 8.0, 0.05% dodecyl-β-D-maltoside at 4 °C overnight. The purified protein was stored in glass vials at 4 °C at a concentration of 25–30 mg/ml.
Purified ba 3 CcO was reconstituted in a lipidic cubic phase (LCP) and microcrystals for serial femtosecond crystallography were produced by optimizing previous crystallization conditions24 following a reportedly successful workflow25. Crystallizations were performed in 9-well glass plates where a string of protein-lipid mixture was added to 300 μl precipitant solution and covered by a sheet of plastics, or in Hamilton glass syringes. Crystals of 5–20 μm in size were obtained within 2–3 days at 19 °C in 37% (v/v) PEG 400, 1.0–1.4 M NaCl, 100 mM sodium cacodylate trihydrate pH 5.3 and stored in Hamilton gastight syringes.
Microcrystals of ba 3 CcO were prepared as described above. 10 μl crystal-containing LCP was added to a CaF2 glass cell with a path length of 0.2 mm and the absorbance spectrum recorded between 200 and 700 nm in a Hitachi U-2910 spectrophotometer. The absorption spectrum of the monoolein mixed with buffer only was subtracted from the spectrum of the samples. To record a spectrum of the reduced sample, sodium dithionite was mixed with monoolein that was subsequently added to the crystal-containing LCP, giving a final dithionite concentration of 4.8 mM. The final protein concentration in the LCP was approximately 0.1 mM for both samples. However, the local crystal concentration in the cell may vary between different measurements.
Data collection and processing
15 μl LCP-crystals of ba 3 CcO were homogenized with 45 μl monoolein (Nu-Check Prep Ink. CAS: 111–03–5) prior to loading into the LCP injector (described in detail in ref. 26). SFX diffraction data was collected at the BL3 beamline at the Spring-8 Angstrom Compact Free Electron Laser (SACLA)44 at 7.6 keV with a repetition rate of 30 Hz and a pulse duration of <10 fs. The X-ray pulses were focused to 1.5 μm (height) ×1.3 μm (width) by Kirkpatrick-Baez mirrors, where the pulse energy at the sample position was estimated at approximately 95 μJ per pulse. The LCP-crystals were injected into the X-ray beam through a 75 μm diameter nozzle at a flow rate of 0.48 μl/min. The sample-to-detector distance was 53 mm and data was recorded using an eight sensor module CCD detector45. Diffraction images were processed using the software package Cheetah27 through a pipeline adapted for SACLA46. Images containing more than 20 diffraction peaks were identified as hits and further processed with CrystFEL28 where DirAx47 and Mosflm48 were used for indexing. Intensities from all images were merged and scaled using Partialator without partiality correction. A per-crystal resolution cut-off was employed that rejected data beyond 1.2 Å past the crystal resolution as calculated by the peak finding in CrystFEL. The crystal hit rate was about 12% and the indexing rate was 72%. The average radiation dose per crystal was calculated using the program RADDOSE-3D49. This can be considered as an upper estimate of the actual dose due to the possibility that photoelectrons escape from the microcrystals, which is not taken into account in the calculation.
Structure determination and refinement
The structure was solved by molecular replacement with Phaser29 using the high-resolution cryo-LCP wild type ba 3 CcO structure (PDB code 3S8F) as a template. The model was built in Coot50 and refinement at a resolution of 2.3 Å was carried out in REFMAC551 using anisotropic displacements with three rigid-body motion groups and an X-ray weighting term of 0.05. Final R work and R free were 16.2% and 19.8% respectively with 99.6% of the side chains within accepted Ramachandran regions. Data collection and refinement statistics are summarized in Table 1.
Kaila, V. R., Verkhovsky, M. I. & Wikstrom, M. Proton-coupled electron transfer in cytochrome oxidase. Chemical reviews 110, 7062–7081, doi:10.1021/cr1002003 (2010).
Adelroth, P., Ek, M. S., Mitchell, D. M., Gennis, R. B. & Brzezinski, P. Glutamate 286 in cytochrome aa3 from Rhodobacter sphaeroides is involved in proton uptake during the reaction of the fully-reduced enzyme with dioxygen. Biochemistry 36, 13824–13829, doi:10.1021/bi9629079 (1997).
Wikstrom, M., Jasaitis, A., Backgren, C., Puustinen, A. & Verkhovsky, M. I. The role of the D- and K-pathways of proton transfer in the function of the haem-copper oxidases. Biochimica et biophysica acta 1459, 514–520 (2000).
Branden, M. et al. On the role of the K-proton transfer pathway in cytochrome c oxidase. Proceedings of the National Academy of Sciences of the United States of America 98, 5013–5018, doi:10.1073/pnas.081088398 (2001).
Ganesan, K. & Gennis, R. B. Blocking the K-pathway still allows rapid one-electron reduction of the binuclear center during the anaerobic reduction of the aa3-type cytochrome c oxidase from Rhodobacter sphaeroides. Biochimica et biophysica acta 1797, 619–624, doi:10.1016/j.bbabio.2010.03.012 (2010).
Chang, H. Y., Hemp, J., Chen, Y., Fee, J. A. & Gennis, R. B. The cytochrome ba3 oxygen reductase from Thermus thermophilus uses a single input channel for proton delivery to the active site and for proton pumping. Proceedings of the National Academy of Sciences of the United States of America 106, 16169–16173, doi:10.1073/pnas.0905264106 (2009).
Kannt, A. et al. Electrical current generation and proton pumping catalyzed by the ba3-type cytochrome c oxidase from Thermus thermophilus. FEBS letters 434, 17–22 (1998).
Iwata, S., Ostermeier, C., Ludwig, B. & Michel, H. Structure at 2.8 A resolution of cytochrome c oxidase from Paracoccus denitrificans. Nature 376, 660–669, doi:10.1038/376660a0 (1995).
Svensson-Ek, M. et al. The X-ray crystal structures of wild-type and EQ(I-286) mutant cytochrome c oxidases from Rhodobacter sphaeroides. Journal of molecular biology 321, 329–339 (2002).
Tsukihara, T. et al. The whole structure of the 13-subunit oxidized cytochrome c oxidase at 2.8 A. Science 272, 1136–1144 (1996).
Soulimane, T. et al. Structure and mechanism of the aberrant ba(3)-cytochrome c oxidase from thermus thermophilus. The EMBO journal 19, 1766–1776, doi:10.1093/emboj/19.8.1766 (2000).
Lyons, J. A. et al. Structural insights into electron transfer in caa3-type cytochrome oxidase. Nature 487, 514–518, doi:10.1038/nature11182 (2012).
Qin, L. et al. Redox-dependent conformational changes in cytochrome C oxidase suggest a gating mechanism for proton uptake. Biochemistry 48, 5121–5130, doi:10.1021/bi9001387 (2009).
Yoshikawa, S. et al. Redox-coupled crystal structural changes in bovine heart cytochrome c oxidase. Science 280, 1723–1729 (1998).
Liu, B. et al. Structural changes that occur upon photolysis of the Fe(II)(a3)-CO complex in the cytochrome ba(3)-oxidase of Thermus thermophilus: a combined X-ray crystallographic and infrared spectral study demonstrates CO binding to Cu(B). Biochimica et biophysica acta 1817, 658–665, doi:10.1016/j.bbabio.2011.12.010 (2012).
Liu, J., Qin, L. & Ferguson-Miller, S. Crystallographic and online spectral evidence for role of conformational change and conserved water in cytochrome oxidase proton pump. Proceedings of the National Academy of Sciences of the United States of America 108, 1284–1289, doi:10.1073/pnas.1012846108 (2011).
Funatogawa, C. et al. Role of the Conserved Valine 236 in Access of Ligands to the Active Site of Thermus thermophilus ba3 Cytochrome Oxidase. Biochemistry 56, 107–119, doi:10.1021/acs.biochem.6b00590 (2017).
Muramoto, K. et al. Bovine cytochrome c oxidase structures enable O2 reduction with minimization of reactive oxygens and provide a proton-pumping gate. Proceedings of the National Academy of Sciences of the United States of America 107, 7740–7745, doi:10.1073/pnas.0910410107 (2010).
Ostermeier, C., Harrenga, A., Ermler, U. & Michel, H. Structure at 2.7 A resolution of the Paracoccus denitrificans two-subunit cytochrome c oxidase complexed with an antibody FV fragment. Proceedings of the National Academy of Sciences of the United States of America 94, 10547–10553 (1997).
Neutze, R., Wouts, R., van der Spoel, D., Weckert, E. & Hajdu, J. Potential for biomolecular imaging with femtosecond X-ray pulses. Nature 406, 752–757, doi:10.1038/35021099 (2000).
Amin, M., Badawi, A. & Obayya, S. S. Radiation Damage in XFEL: Case study from the oxygen-evolving complex of Photosystem II. Sci Rep 6, 36492, doi:10.1038/srep36492 (2016).
Nass, K. et al. Indications of radiation damage in ferredoxin microcrystals using high-intensity X-FEL beams. J Synchrotron Radiat 22, 225–238, doi:10.1107/S1600577515002349 (2015).
Chapman, H. N. et al. Femtosecond X-ray protein nanocrystallography. Nature 470, 73–77, doi:10.1038/nature09750 (2011).
Tiefenbrunn, T. et al. High resolution structure of the ba3 cytochrome c oxidase from Thermus thermophilus in a lipidic environment. PloS one 6, e22348, doi:10.1371/journal.pone.0022348 (2011).
Liu, W., Ishchenko, A. & Cherezov, V. Preparation of microcrystals in lipidic cubic phase for serial femtosecond crystallography. Nat Protoc 9, 2123–2134, doi:10.1038/nprot.2014.141 (2014).
Nango, E. et al. A three-dimensional movie of structural changes in bacteriorhodopsin. Science 354, 1552–1557, doi:10.1126/science.aah3497 (2016).
Barty, A. et al. Cheetah: software for high-throughput reduction and analysis of serial femtosecond X-ray diffraction data. Journal of applied crystallography 47, 1118–1131, doi:10.1107/S1600576714007626 (2014).
White, T. A. et al. CrystFEL: a software suite for snapshot serial crystallography. Journal of applied crystallography 45, 335–341, doi:10.1107/S0021889812002312 (2012).
McCoy, A. J. et al. Phaser crystallographic software. Journal of applied crystallography 40, 658–674, doi:10.1107/S0021889807021206 (2007).
Koepke, J. et al. High resolution crystal structure of Paracoccus denitrificans cytochrome c oxidase: new insights into the active site and the proton transfer pathways. Biochimica et biophysica acta 1787, 635–645, doi:10.1016/j.bbabio.2009.04.003 (2009).
Hirata, K. et al. Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL. Nature methods 11, 734–736, doi:10.1038/nmeth.2962 (2014).
Kaila, V. R. et al. A combined quantum chemical and crystallographic study on the oxidized binuclear center of cytochrome c oxidase. Biochimica et biophysica acta 1807, 769–778, doi:10.1016/j.bbabio.2010.12.016 (2011).
Liu, B. et al. Combined microspectrophotometric and crystallographic examination of chemically reduced and X-ray radiation-reduced forms of cytochrome ba3 oxidase from Thermus thermophilus: structure of the reduced form of the enzyme. Biochemistry 48, 820–826, doi:10.1021/bi801759a (2009).
Sakaguchi, M., Shinzawa-Itoh, K., Yoshikawa, S. & Ogura, T. A resonance Raman band assignable to the O-O stretching mode in the resting oxidized state of bovine heart cytochrome c oxidase. J Bioenerg Biomembr 42, 241–243, doi:10.1007/s10863-010-9282-y (2010).
Hellwig, P., Soulimane, T., Buse, G. & Mantele, W. Electrochemical, FTIR, and UV/VIS spectroscopic properties of the ba(3) oxidase from Thermus thermophilus. Biochemistry 38, 9648–9658, doi:10.1021/bi9903401 (1999).
von Ballmoos, C. et al. Mutation of a single residue in the ba3 oxidase specifically impairs protonation of the pump site. Proceedings of the National Academy of Sciences of the United States of America 112, 3397–3402, doi:10.1073/pnas.1422434112 (2015).
Yano, N. et al. The Mg2+ -containing Water Cluster of Mammalian Cytochrome c Oxidase Collects Four Pumping Proton Equivalents in Each Catalytic Cycle. The Journal of biological chemistry 291, 23882–23894, doi:10.1074/jbc.M115.711770 (2016).
Wiertz, F. G. et al. An oxo-ferryl tryptophan radical catalytic intermediate in cytochrome c and quinol oxidases trapped by microsecond freeze-hyperquenching (MHQ). FEBS letters 575, 127–130, doi:10.1016/j.febslet.2004.08.048 (2004).
Paulus, A., Werner, C., Ludwig, B. & de Vries, S. The cytochrome ba3 oxidase from Thermus thermophilus does not generate a tryptophan radical during turnover: Implications for the mechanism of proton pumping. Biochimica et biophysica acta 1847, 1093–1100, doi:10.1016/j.bbabio.2015.05.013 (2015).
Lu, J. & Gunner, M. R. Characterizing the proton loading site in cytochrome c oxidase. Proceedings of the National Academy of Sciences of the United States of America 111, 12414–12419, doi:10.1073/pnas.1407187111 (2014).
Barends, T. R. et al. Direct observation of ultrafast collective motions in CO myoglobin upon ligand dissociation. Science 350, 445–450, doi:10.1126/science.aac5492 (2015).
Tenboer, J. et al. Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein. Science 346, 1242–1246, doi:10.1126/science.1259357 (2014).
Chen, Y., Hunsicker-Wang, L., Pacoma, R. L., Luna, E. & Fee, J. A. A homologous expression system for obtaining engineered cytochrome ba3 from Thermus thermophilus HB8. Protein expression and purification 40, 299–318, doi:10.1016/j.pep.2004.11.014 (2005).
Tono, K. et al. Beamline, experimental stations and photon beam diagnostics for the hard x-ray free electron laser of SACLA. New Journal of Physics 15, 30358 (2013).
Kameshima, T. et al. Development of an X-ray pixel detector with multi-port charge-coupled device for X-ray free-electron laser experiments. Rev Sci Instrum 85, 033110, doi:10.1063/1.4867668 (2014).
Nakane, T. et al. Data processing pipeline for serial femtosecond crystallography at SACLA. Journal of applied crystallography 49, 1035–1041, doi:10.1107/S1600576716005720 (2016).
Duisenberg, A. J. M. Indexing in Single-Crystal Diffractometry with an Obstinate List of Reflections. Journal of applied crystallography 25, 92–96, doi:10.1107/S0021889891010634 (1992).
Battye, T. G. G., Kontogiannis, L., Johnson, O., Powell, H. R. & Leslie, A. G. W. iMOSFLM: a new graphical interface for diffraction-image processing with MOSFLM. Acta Crystallogr D 67, 271–281, doi:10.1107/S0907444910048675 (2011).
Zeldin, O. B., Gerstel, M. & Garman, E. F. RADDOSE-3D: time- and space-resolved modelling of dose in macromolecular crystallography. Journal of applied crystallography 46, 1225–1230, doi:10.1107/S0021889813011461 (2013).
Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. Features and development of Coot. Acta crystallographica. Section D, Biological crystallography 66, 486–501, doi:10.1107/S0907444910007493 (2010).
Murshudov, G. N. et al. REFMAC5 for the refinement of macromolecular crystal structures. Acta crystallographica. Section D, Biological crystallography 67, 355–367, doi:10.1107/S0907444911001314 (2011).
We thank Prof. Robert Gennis, University of Illinoi, and Profs. Peter Brzezinski and Pia Ädelroth, Stockholm University, for kindly providing the ba 3 CcO strain. We thank Dr. Matthijs Panman and Dr. Joachim Kübel, University of Gothenburg, for assistance with the crystal absorbance measurements. XFEL experiments were conducted at BL3 of SACLA with the approval of the Japan Synchrotron Radiation Research Institute (JASRI) (proposal numbers 2015B8054 and 2016B8031). We are grateful for assistance by the staff of SACLA and SPring-8, and acknowledge computational support from SACLA HPC system and Mini-K super computer system. This work was supported by the X-ray Free-Electron Laser Priority Strategy Program (MEXT). RN acknowledges funding from the Swedish Research Council (grants 2015–00560 and 349-2011-6485), the Swedish Foundation for Strategic Research (grant SRL10-0036), the Knut and Alice Wallenberg Foundation (grants KAW 2012.0284 and KAW 2014.0275) and the European commission Marie Curie Training Networks (NanoMem and X-probe).
The authors declare that they have no competing interests.
Accession codes: The coordinate and structure factor files have been deposited with the Protein Data Bank under accession code 5NDC.
Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
About this article
Applied Sciences (2019)
Inorganic Chemistry (2019)
Chemical Reviews (2018)
Chemical Reviews (2018)