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Analysis of survival and hatching transcriptomes from potato cyst nematodes, Globodera rostochiensis and G. pallida

Scientific Reportsvolume 7, Article number: 3882 (2017) | Download Citation


Potato cyst nematodes (PCNs), Globodera rostochiensis and G. pallida, cause important economic losses. They are hard to manage because of their ability to remain dormant in soil for many years. Although general knowledge about these plant parasitic nematodes has considerably increased over the past decades, very little is known about molecular events involved in cyst dormancy and hatching, two key steps of their development. Here, we have studied the progression of PCN transcriptomes from dry cysts to hatched juveniles using RNA-Seq. We found that several cell detoxification-related genes were highly active in the dry cysts. Many genes linked to an increase of calcium and water uptake were up-regulated during transition from dormancy to hydration. Exposure of hydrated cysts to host plant root exudates resulted in different transcriptional response between species. After 48 h of exposure, G. pallida cysts showed no significant modulation of gene expression while G. rostochiensis had 278 differentially expressed genes. The first G. rostochiensis significantly up-regulated gene was observed after 8 h and was coding for a transmembrane metalloprotease. This enzyme is able to activate/inactivate peptide hormones and could be involved in a cascade of events leading to hatching. Several known effector genes were also up-regulated during hatching.


Potato cyst nematodes (PCNs), Globodera rostochiensis and G. pallida, are major plant-parasitic nematodes of potato and are found infesting fields alone or as mixtures of both species1. They are present in the major world potato production areas and are quarantine organisms in many countries2, 3. Yield losses are usually proportional to initial soil contamination4, 5 and are estimated at 2 t/ha of potatoes for every 20 eggs/g of soil6. For G. rostochiensis, an initial population density as low as 0.1 J2/cm3 of soil can significantly reduce potato yields7. These damages will depend on several factors including soil type, interactions with microorganisms, differences in husbandry, cultivars, weather and potential yields between sites8. However, yield losses over 50% have been reported8. PCNs can also attack other crops (tomato, eggplant) and several Solanaceaeous weeds such as nightshades, which can serve as reservoirs9, 10. G. rostochiensis and G. pallida are members of the Heteroderidae and originate from South America. They were probably introduced to Europe along with potato breeding material around 185011.

Like other specialized parasites, the PCN life cycle is synchronized with their hosts to optimize the chances of successful invasion12. This synchrony is possible because PCN unhatched juveniles have the ability to remain dormant until a stimulus from a host is perceived, indicating favourable conditions for hatching. PCN eggs are trapped inside the dead female body, forming the cyst structure, and can survive in soil for over 20 years13. Hatching occurs in response to root diffusate from a suitable Solanaceae growing nearby. However, some eggs will only hatch on restimulation, a strategy to increase population persistence throughout growing seasons and to lower competition between hatched juveniles12. Variable spontaneous hatching also occurs, depending on field conditions14.

The PCN hatching process consists of three main steps: increase of the eggshell permeability, activation of the larva and eclosion15. Trehalose inside the eggs is associated with survival and hatching. The osmotic stress caused by the accumulation of this sugar will inhibit locomotion and induced quiescence, thus providing protection against environmental stresses16. The hatching process starts with a permeability change of the eggshell lipid layer involving Ca2+  17, and subsequent leakage of trehalose in response to host root diffusates18. With the loss of osmotic pressure, water uptake will allow juvenile larvae to rehydrate and to restore motility. Active larvae will cut the eggshell and hatch. Changes in the lipid content and fatty acid composition of the larvae also occur in the egg after exposure to potato root diffusate19. A number of external environmental factors, including host plant root diffusates, soil temperature and moisture, soil oxygen, soil microorganisms, minerals and organic substances, can serve as hatch inducers or can influence hatching20. Natural compounds (i.e. solanoeclepin A) and synthetic analogues21 and other chemicals such as picrolonic acid, sodium thiocyanate, alpha-solanine, and alpha-chaconine partially stimulate the hatching process, with greater hatching levels for G. rostochiensis than for G. pallida 22. Using potato root diffusate (PRD), Perry and Beane23 showed that a single 5-min exposure was enough to induce hatching of G. rostochiensis eggs. In contrast, G. pallida eggs required weekly 5-min exposures to PRD to induce hatching24. In an experiment to establish the relationship between soil temperature and PCN hatching, Kaczmarek et al.25 have shown that G. rostochiensis hatched more quickly than G. pallida and that hatching of both species increases with temperature with a peak around 20 °C.

The series of physiological and behavioral events associated with hatching strongly suggest that changes in gene expression may be involved in the process. However, very little is known about which genes are important to cyst survival and hatching. Jones, et al.26, using differential display as analytical technique, did not find any changes in gene expression linked to exposure to PRD in G. rostochiensis. On the other hand, they found a few differentially expressed genes (DEGs) associated with cyst survival, but none of them showed significant homology to known sequences. Similarly, Qin, et al.27 highlighted a few coding sequences by cDNA-AFLP related to G. rostochiensis cyst survival and pathogenicity.

Other studies have showed indirect observations of increased transcriptional activity during hatching of PCNs. Perry28, as well as Atkinson, et al.16, found an accumulation of secretory granules and an increase of the nucleolus size of the dorsal oesophageal glands of G. rostochiensis within a few hours of exposure to PRD. Likewise, Blair29 found an increase in staining of a nucleic acid specific dye in unhatched second stage juveniles of G. rostochiensis after three days of exposure to tomato root diffusate (TRD).

A recent transcriptome analysis of G. pallida has shown that 526 genes were up-regulated at the transition from encysted eggs (containing dormant J2s) and hatched J2 nematodes30. This large-scale activation of transcription is indicative of the numerous metabolic changes that are associated with the up-regulation of genes involved in root penetration and the production of other secreted proteins interacting with plant defense mechanisms. Additionally, Palomares-Rius, et al.31 showed significant changes in gene expression after hydration of quiescent eggs of G. pallida and after exposure to TRD using a microarray platform. However, this study was done after soaking cysts for 4 days in TRD.

Here, we studied the changes in transcriptomic activity of PCN from dormant to hatched juveniles using RNA-Seq, a high throughput/hi-resolution technique. Two independent studies, one for G. rostochiensis and one for G. pallida, were combined to present shared pathways. These studies were designed to capture early gene activation during hatching. The objectives of this work were to analyze the genes involved in hatching, to identify those necessary for survival and to compare gene expression in two closely related species, G. rostochiensis and G. pallida during these key events of their life cycle. In a context of pesticide withdrawal, this knowledge is of vital importance for the design of new integrated pest management strategies.


De novo transcriptome assemblies

RNA-Seq library sequencing yielded 511 M reads for G. rostochiensis and 213 M reads for G. pallida. A total of 239 k components were de novo-assembled by Trinity from the G. rostochiensis reads (assembly statistics are summarized in Table S1). This high number was attributable to the presence of sequences from contaminants. In order to remove the contaminating components from the G. rostochiensis transcriptome, we developed a decontamination method called Contaminant Contigs Removal by Counts (CCRbC, summarized in Material and Methods). This step removed 61% of the components, leaving 93,089 final contigs, about three times more contigs compared to the G. pallida transcriptome.

In comparison, the G. rostochiensis reference transcriptome has 14,309 contigs32 and the G. pallida reference transcriptome has 16,417 contigs30. Only 19.1% of the G. rostochiensis de novo contigs had BLAST hits (e-value < 1e−10) on its reference transcriptome, but these contigs actually covered 96.9% of the reference transcriptome. For G. pallida, 70% of the de novo contigs had a BLAST hit on its reference transcriptome and these contigs covered 82.7% of the reference transcriptome.

Differentially expressed genes analyses

DEGs for G. rostochiensis and G. pallida de novo transcriptomes and reference transcriptomes are summarized in Fig. 1 and detailed in Tables S2 to S5. For both PCNs, there were more DEGs found using the de novo transcriptomes than the reference transcriptomes. The ratio of up- and down-regulated genes in each treatment was similar between Trinity and reference analyses for both species. However, G. pallida had more up-regulated than down-regulated genes in the dry cysts and the opposite at all hatching time-points (5 h, 24 h and 48 h of exposure to TRD). Conversely, G. rostochiensis had more down-regulated than up-regulated genes in the dry cysts and the opposite during hatching (24 h, 48 h and 7 days of exposure to PRD).

Figure 1
Figure 1

Pairwise counts of differentially expressed genes (DEGs) in each treatment of each transcriptome. The control treatment “water” represents hydrated cyst and time represent the soaking time in PRD/TRD after hydration.


Many contigs were differentially expressed in the dry cysts in comparison to hydrated cysts. Using the de novo transcriptomes to measure gene expression, we found 592 up- and 952 down-regulated contigs for G. rostochiensis and 1436 up- and 813 down-regulated contigs for G. pallida in dry cysts (Fig. 1). Interestingly, seven putative survival-related DEGs with similar upregulated expression patterns had homologs in all four transcriptomes (Table 1). Similarly, eight of the genes upregulated after cyst hydration were common to all transcriptomes (Table 2). BLAST results for all these contigs can be found in Supplemental Tables S2S5.

Table 1 Differentially expressed genes (DEGs, P < 0.05, FDR < 0.1) up-regulated in dry cysts common to both G. rostochiensis and G. pallida in both de novo and reference transcriptomes.

Table 2 Differentially expressed genes (DEGs, P < 0.05, FDR < 0.1) up-regulated in hydrated cysts common to both G. rostochiensis and G. pallida in both de novo and reference transcriptomes.


The first G. rostochiensis transcript to be significantly up-regulated in both Trinity and reference transcriptomes, i.e. after 8 h exposure to PRD, was encoding for a protein similar to the neprilysin NEP-1 (comp140896_c0, Fig. 1 and Tables S2 and S3). The expression pattern of this gene was confirmed by RT-qPCR (Fig. 2). Also, 39 contigs common to both G. rostochiensis de novo and reference transcriptomes were significantly up-regulated during hatching treatments (cysts soaked for 8 h, 24 h and 48 h in PRD; Table 3). No DEGs were shared in both G. pallida transcriptomes in response to hatching treatments (up to 48 h). No hatched J2 were observed across time-course except a few individuals of G. rostochiensis after 7 days of exposure to PRD.

Figure 2
Figure 2

Expression of neprilysin gene nep-1 by RT-qPCR in dry cysts, hydrated eggs, and hydrated eggs exposed to potato root diffusate (PRD) for 15 min, 1 h, 8 h, 24 h, 48 h and 7 days and hatched J2. Hydrated cyst expression level was use as calibrator. Error bars represent standard error of the mean and significant differences among treatments are indicated by different letters (Tukey).

Table 3 Differentially expressed genes (DEGs, P < 0.05, FDR < 0.1) up-regulated after hatching 8 h, 24 h or 48 h exposure to potato root diffusate common to G. rostochiensis de novo and reference transcriptomes.

DEGs clustering

The 4,094 unique DEGs obtained with the G. rostochiensis Trinity transcriptome were clustered in 195 groups. Among them, one cluster showed a clear survival-related expression pattern (Fig. 3B), i.e. high expression levels in dry cysts followed by a decrease in all other treatments. This cluster contained 10 DEGs listed in Table S7. Similarly, one other cluster had a clear hatching-specific expression pattern (Fig. 3D) with low expression levels in dry and hydrated cysts followed by an increase in expression in early contact to PRD, then a plateau and finally a decrease in expression in the hatched J2s. The 13 DEGs from this cluster were up-regulated in at least one hatching treatment (Table S9).

Figure 3
Figure 3

G. rostochiensis expression clusters of interest harboring differentially expressed genes (DEGs) identified with the Trinity transcriptome. (A) Cluster containing the trehalose 6-phosphate synthase gene. (B) Cluster with a survival-specific expression pattern. (C) Cluster containing the nep-1 gene. (D) Cluster with a hatching-specific expression pattern.

Clusters were also searched for candidate genes involved in survival (trehalose 6-phosphate synthase; Fig. 3A) and hatching (NEP-1; Fig. 3C). The cluster containing the trehalose 6-phosphate synthase had 31 DEGs up-regulated in dry cysts (Table S6). The cluster with the gene encoding for NEP-1 (Fig. 3C), was up-regulated in both G. rostochiensis transcriptomes in response to hatching factors, and harboured 11 genes (Table S8).


Throughout their coevolution with their hosts, cyst nematodes have developed remarkable abilities to ensure reproduction success and species persistence. One of the most impressive strategies is the ability of potato cyst nematodes, Globodera rostochiensis and G. pallida, to synchronize their hatching with the presence of a suitable host and to survive in soil for several years13. Very little is known about the genetic control behind long-term dormancy and hatching. In this work, we have highlighted important genetic pathways that are activated during these key life stages using RNA-Seq. Sequence contamination from soil/cyst microorganisms was found to be a big challenge for data analysis. For G. rostochiensis, more than 60% of the transcripts obtained were contaminant sequences. A simple yet efficient decontamination algorithm (CCRbC) was developed and applied to remove most of these contaminating sequences without losing important information. Indeed, a horizontal coverage of 96.9% was obtained by aligning the remaining transcripts to the reference transcriptome.

During dormancy, cyst nematodes are anhydrobiotic, surviving almost completely desiccated33. In addition, to provide a physical protection, the cyst envelope and eggshell slow down the water loss rate during desiccation, which is thought to be very important for cryptobiosis survival34. Trehalose accumulation inside the larvae body is also important for long-term survival. It may replace bound water by attaching to polar side groups of proteins and phospholipids, thus maintaining the balance between hydrophilic and hydrophobic forces acting on the molecules and preventing them from collapsing15. In this study, we have found that the expression of a trehalose 6-phosphate synthase gene was up-regulated in dry cysts in comparison with hydrated cysts in G. rostochiensis (Table S8). The gene encoding the enzyme with the opposite biochemical function, trehalase, which catalyzes the conversion of trehalose to glucose, was found to be up-regulated in dry cysts of G. pallida. This observation may be attributable to the fact that after its synthesis in early phases of cryptobiosis, trehalose will later serve as an energy source. Overexpression of a trehalase in G. pallida could reflect its use. In the specific case of G. pallida, the cysts were stored at 4 °C, in order to treat for diapause, for more than one year. Thus, they could have started to use their reserves in order to survive longer. This could also be one of the reasons behind the up-regulation pattern in G. pallida during this stage. Our results reflect the importance of trehalose metabolism during dormancy. Of course, other cellular mechanisms are required to ensure cyst survival during extended desiccation periods34.

The desiccation state found in dormant cysts impairs normal reactive oxygen species (ROS) detoxification mechanisms35. Molecules such as superoxide \(({{\rm{O}}}_{2}^{\cdot -})\), hydroxyl (·OH) radicals and peroxide (H2O2) are highly reactive and can damage nucleic acids, proteins and lipids, leading to premature aging or death of cells36. Antioxidants are the main molecules capable of balancing ROS levels35. Interestingly, we found that several enzymatic antioxidant pathways were up-regulated in dry cysts. One of the most common superoxide radical scavengers, superoxide dismutase (comp252050_c0), as well as a dehydrogenase (comp209610_c0), were indeed up-regulated in dry cysts (compared to hydrated cysts) in both nematode species, within all four transcriptomes (Table 1). Moreover, a gene coding for a protein similar to the THI4 thiazole biosynthetic enzyme (comp238116_c0, Table 1) was also up-regulated in dry cysts in both nematodes in both de novo and reference transcriptomes. Thiazol is the thiamin (vitamin B1) precursor. Since vitamin B1 has strong antioxidant properties, it could play an important ROS detoxifying role under anhydrobiotic conditions37. Most animals do not have the machinery to synthesize complex B vitamins as they can easily find it through their diet. Complex B vitamin biosynthesis genes are found in other cyst nematodes. For example, Craig, et al.38 found vitamin B6 biosynthesis genes in Heterodera glycines, the soybean cyst nematode. Additional genes involved in vitamin B1, B5 and B7 biosynthesis with evidence of horizontal gene transfer from bacteria are also found in H. glycines 39. The authors suggested that they could have a protective role for the nematode through their antioxidant properties. These genes were also recently identified in G. pallida 30 and G. rostochiensis 32 genomes.

Another gene (comp204557_c0; Table S7) coding for a selenoprotein (thioredoxin) was overexpressed in G. rostochiensis dry cysts. This protein also harbours antioxidant properties and was found to play an important role in aging and longevity in different organisms40,41,42. Selenoproteins contain the rare amino acid selenocysteine, encoded by the UGA codon usually coding for translation termination, combined with a special mRNA structure called selenocysteine insertion sequence 43. Interestingly, thioredoxin reductase is the only selenoprotein ever reported in nematodes44 and one of the only enzymes with peroxidase activity known in nematodes. This gene, which showed the highest fold change in our study, has been labeled essential for life in many organisms and is currently a promising target for antiparasitic drugs development against nematodes in humans45. Also of special interest, several genes implicated in post-transcriptional regulation and coding for RNA-binding proteins and histone deacetylases were up-regulated in dry cysts.

Both PCNs showed important changes in gene expression in response to hydration. Many of those changes were related to calcium, which is essential for PCN hatching17. Our results further support the importance of calcium in early steps of hatching. Among the up-regulated genes (Table 2), gcy-9 (comp242611_c0) coding for a guanylyl cyclase was of special interest because it is part of a signaling cascade activated by low levels of intracellular calcium leading to the synthesis of cyclic guanosine monophosphate (cGMP), which in turn allows the entry of calcium into the cell46. Atkinson, et al.16 previously showed that intracellular levels of cyclic adenosine monophosphate (cAMP) and cGMP influence hatching of G. rostochiensis. An increased level of cGMP caused by gcy-9 overexpression in response to hydration could prepare the cells for a better reactivity to hatching factors, which act in a calcium-mediated way47.

Overexpression of the gene encoding the transmembrane protein four domain-type voltage-gated ion channel alpha-1 subunit (comp257544_c2) could also play a role by restoring the permeability of the cell membranes to calcium, as well as the overexpression of the del gene coding for a cation channel protein (comp252939_c1). The gene mua-3 (comp258240_c1) was also up-regulated during hydration, which is predicted to have a calcium ion-binding activity. Palomares-Rius, et al.31 also found that expression of many transmembrane transporter genes was significantly increased when hydrated G. pallida cysts were exposed to TRD.

Effector genes were upregulated during the water uptake phase, like a beta-endoglucanase (comp242752_c0) known to be an important effector for host root infection48. Goellner, et al.49 also found that expression of this gene was increased prior hatching of Globodera tabacum eggs.

In comparison to G. rostochiensis, G. pallida eggs normally take longer to hatch in response to root diffusate25, 50. Our results are consistent with this observation, with the first few DEGs observed after 48 h of exposure to TRD for G. pallida. In contrast, by 48 h of exposure to PRD, 278 different DEGs were upregulated in G. rostochiensis. For G. pallida, Palomares-Rius, et al.31 confirmed that several hundred genes were differentially expressed after 4 days of exposure to TRD.

The first DEG was observed after 8 h of exposure to PRD in G. rostochiensis, a gene coding for a neprilysin (comp140896_c0; Table 3), was up-regulated whether the reference or de novo transcriptomes was used to measure gene expression. Neprilysins (NEPs) are transmembrane zinc-metalloproteases that are well conserved throughout the animal kingdom. They were first identified in nematodes by Sajid and Isaac51. NEPs are able to hydrolyse peptide bonds at the N terminus of hydrophobic amino acids of a variety of substrates (e.g. enkephalins, tachykinins, neurotensins) thereby not only allowing the degradation of peptides, but also the post-transcriptional modification of inactive precursor peptides52. In Caenorhabditis elegans, NEP-1 is involved in locomotion and pharyngeal pumping and is highly expressed prior to hatching52. More than 20 putative neprilysin genes were identified in C. elegans 53. Here, we found 11 different transcripts for NEPs. Many of them were highly expressed during the early phases of the life cycle (Fig. S1), although stronger expression levels were observed in the later stages. The homolog of this gene in G. pallida (GPLIN_000276000) is also up-regulated 4 days after TRD exposure31, which points to a common hatching mechanisms between both species, but later in G. pallida. Other Zn2+-metalloproteases could also play a significant role in hatching, such as the matrix metalloproteinase in Heterodera glycines (Hg-MMP) identified by Kovaleva, et al.54.

The gene cht-2 (comp258474_c0; Table 3) coding for a chitinase was up-regulated at 24 h and 48 h following exposure to PRD. Chitinases catabolize the β-1,4-N-acetyl-D-glucosamine polysaccharide chitin, a compound absent from PCN hosts and only found in the eggshell of plant-parasitic nematodes. Similar endochitinases were previously identified in the soybean cyst nematode, H. glycines 55 and in preparasitic southern root-knot nematodes, Meloidogyne incognita 56.

Several polysaccharide-degrading enzymes genes were up-regulated during hatching (Table 3). Most of them are essential for plant colonization and prepare the nematode for its infective stage. For example, beta-endoglucanases (comp242752_c0 and comp258555_c1), beta-levanase invertase (comp219369_c0) and arabinogalactan endo-beta-galactosidase (comp252640_c1) are cell wall degradation enzymes overexpressed in response to root diffusate. The arabinogalactan endo-beta-galactosidases, which hydrolyse arabinogalactans found in dicot cell walls, may be specific to plant-parasitic nematodes of the cyst nematode group as it is present in G. pallida and H. schachtii, but absent from M. incognita and M. hapla 30, 57.

Many phosphatase genes were also up-regulated in response to root diffusate at various times of exposure, including histidine acid phosphatase (comp241201_c2). The product of this gene catalyses the breakdown of phytate (inositol hexakisphosphate), an important phosphorus storage compound in many plants. A recent study has shown that down-regulation of myo-inositol phosphate synthase in plants reduces its susceptibility to cyst nematodes58.

Multiple genes coding for peptidases (comp239365_c0, comp250308_c0, comp208748_c0 and comp212021_c0) other than NEPs were also up-regulated during hatching of G. rostochiensis, using both reference and de novo transcriptomes. Some of them are found in G. rostochiensis oral secretions59 and are involved in the hatching process in different nematodes, including G. pallida in later temporal points60, 61. It has been proposed that secreted peptidases could play a role in parasitism in plant-parasitic nematodes62. This family of enzymes is known to contribute to host specificity, host range and virulence in animal-parasitic nematodes63.

Numerous effector genes were up-regulated in response to hatching treatments in both G. rostochiensis Trinity and reference transcriptomes (Tables 3 and S9), including expansin (comp249939_c0), pectate lyases (comp146670_c0 and comp79822_c0) and rbp-1 (comp249497_c3). Pectate lyases are essential for breaking down plant cell walls and were believed to be absent from animals before being found in G. rostochiensis 64. G. rostochiensis pectate lyases and expansin proteins induce strong phenotypes when expressed in planta, suggesting virulence functions65. RBP-1 is similar to a gene previously identified in G. pallida by Blanchard, et al.66 that contains a SPRY domain and a signal peptide and was strongly suspected to be involved in parasitism. This protein was later identified as the avirulence factor recognised by the potato resistance protein Gpa267. Combined with the high level of polymorphism found among the Ran binding proteins68, this finding suggests that this gene family may be under strong selection pressure to evade recognition by the hosts. In the present study, 66 different transcripts with RBP-1 BLAST results were identified, in accord with the previously described high genetic diversity. Jones, et al.69 suggested that alternative splicing might be involved to create a high potential for adaptation in this gene.

In conclusion, we showed that the quiescent state of PCN is active in terms of gene expression. Many genes involved in cell detoxification are up-regulated in both PCN species. Considerable hatching-associated changes occurred during the hydration phase, based on gene expression evidences, including changes in cell permeability and calcium and cGMP levels. Exposure to root diffusate only affected a small number of genes in the early stages of hatching. Among these genes, several transmembrane metalloproteases genes, including NEP-1, were up-regulated and will certainly require further investigation in the future.


Root diffusates

For G. rostochiensis, potato plants cv. Snowden were grown in perlite, in 2 L containers, until they reached about 15 cm-high. At this point, PRD was harvested once a week, for six consecutive weeks, by the method of Fenwick70. Briefly, soil was drenched with tap water until saturation. An extra 50 mL of tap water was then added to the pot and the flowing liquid was collected. The collected liquid was used to repeat this procedure two more times. The final collected liquid was filtered (KenAG, D-547) to obtain PRD. PRD samples were kept at 4 °C in dark plastic bottles until the last one was harvested. Then, all six weekly-sampled PRDs were pooled, freeze-dried and stored at −20 °C. Final volume was recorded prior lyophilization, as well as final weight after lyophilization, for proper PRD reconstitution. PRD was reconstituted from powder with nanopure water at a final concentration of 0.5× and passed through a 0.2 µm filter prior use.

For G. pallida, tomato plants cv. MoneyMaker were grown in 6-inch pots containing Levington Bio-Multicompost (a mixture of sand, soil and peat). When plants reached 4-weeks old, roots were removed carefully from compost, washed and placed in 250 ml beakers with distilled water. After an incubation period of 4 h, roots were removed and the remaining diffusate filtered using Whatman no. 1 filter paper. Filtered TRD was kept at 4 °C and used within 1 week.

Sample description

G. rostochiensis cysts were recovered by flotation71 from soil samples collected in the fall 2011 in Saint-Amable (Quebec, Canada). Cysts were stored dried for at least one year in the dark at room temperature prior to hatching experiments. A time course experiment was set up to study the composition of the transcriptome of G. rostochiensis during quiescence and hatching. The following physiological stages (treatments) were studied: dry cysts, cysts soaked in water for one week (hydration), hydrated cysts soaked in PRD for 15 min, 1 h, 8 h, 24 h, 48 h and 7 d and hatched J2 juveniles. Each cyst sample contained 1000 cysts placed in a mesh bag (Ankom, F57). Cysts were soaked in 30 mL of filtered (0.2 µm) tap water or 0.5× PRD, in a petri dish. Water and PRD were changed every day. All the assays were incubated at 20 °C in an environmental chamber. No hatching occurred during the hydration period. Hatched J2s were harvested daily for a two-week period and pooled for further analysis. Thus, all the treatments, except hatched J2, did not contain any larvae. The experiment was repeated two times.

G. pallida cysts were from the Lindley population maintained by the James Hutton institute. They were multiplied in glasshouse on the susceptible potato cultivar Désirée growing in a mixture of sand and loam (2:1). Temperature was maintained at 20 ± 1 °C with RH between 60–90%, and a 14-h photoperiod. Cysts were extracted from the soil by flotation in pail by thoroughly mixing infested soil with water. Water was then filtered through a 750 µm-pore sieve nested over a 150 µm-pore sieve. Cysts recovered on the second sieve were kept at 4 °C for at least 3 months. Cysts were soaked in filtered (0.2 µm) tap water for 4 days and subsequently transferred to TRD for 5, 24 or 48 h. All the assays were incubated at 20 °C in an environmental chamber. Cysts were crushed using a tissue homogenizer to release eggs. All the debris were removed from eggs by filtration through a 100 µm-pore sieve nested over a 5 µm-pore sieve. Eggs were concentrated by centrifugation. The experiment was repeated two times.

Total RNA extraction, library preparation and sequencing

For G. rostochiensis, cysts soaked in PRD were washed thoroughly with distilled water prior to RNA extraction to remove as much potential contamination as possible. Samples were homogenized in 700 µL of RTL plus buffer with one 6 mm zirconium bead and ~150 µL of 1 mm zirconium beads using the PowerLyzer 24 homogenizer (MO BIO, Carlsbad, CA, USA) and stored at −80 °C until RNA purification. Total RNA was extracted using the RNeasy Plus mini kit (Qiagen, Mississauga, Canada) according to manufacturer’s instructions. Total RNA samples were store at −80 °C prior RNA-Seq library preparation. RNAs were quantified with the NanoDrop 2000 (Thermo Scientific). RNA integrity was assessed with the Bioanlalyzer 2100 (Agilent Technologies) using the RNA 6000 Nano kit. All RNA samples had a RIN value higher than 7 and a 260/230 ratio value over 2.

Library preparation and sequencing were performed at McGill University and Génome Québec Innovation Centre (Montreal, Canada) using the TruSeq RNA sample prep kit v2 (Illumina) and a HiSeq 2000 sequencer (Illumina). For each replicate, all nine samples were multiplexed and sequenced in one lane for 100 bp paired-end reads.

For G. pallida, total RNA was extracted using RNeasy Plus Micro Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions. DNA digestion was conducted on column during RNA extraction using RNase-Free DNase set (Qiagen, Hilden, Germany), as recommended. All RNA samples had a RIN value higher than 7 and a 260/230 ratio value over 2. Total RNA was quantified using a 2100 Bioanalyzer (Agilent Technologies) and the Small RNA kit (Agilent Technologies) following the manufacturer’s instructions. Libraries and sequencing were produced and sequenced in Sanger Institute facilities. Illumina transcriptome libraries were produced using polyadenylated mRNA purified from total RNA with size selection using the Caliper LabChip XT.

Sequence processing, de novo assembly, differential expression analysis and transcript annotation

Reads were trimmed from the 3′ end with a minimal phred score of 30 using the Trimmomatic software72 et al. Illumina sequencing adapters were removed. Trimmed reads shorter than 32 bp were discarded and orphan reads were kept for the assembly.

The Trinity assembler73, 74 was used on normalized trimmed reads (30X coverage) to create de novo transcriptomes for both G. rostochiensis and G. pallida using default parameters. Minimum contig length was set to 300. A custom script was applied to the Trinity transcriptomes to keep only the longest isoform of each component. Then, we applied an in-house algorithm named Contaminant Contig Removal by Counts (CCRbC) to remove contaminant sequences in the transcriptomes. CCRbC uses as input the counts matrix (n x m) produced by RSEM75, where the n contigs are represented by n rows and the r replicates of t treatments are represented by r*t = m columns. The first step is to sum all treatments together for each replicate and for each contig, which resulted in a n by r matrix. Non-contaminant contigs are those that have at least one count for every replicate. Contaminant contigs are removed by cutting rows that contain at least one zero in the n by r matrix. Differential expression (DE; P < 0.05 FDR-corrected at 10%) was measured using the DESeq2 Bioconductor package76 in R statistical software77 using a parametric Wald test. RSEM software was also used to count gene expression on G. pallida 30 and G. rostochiensis 32 reference transcriptomes. Both reference transcriptomes were obtained with the gene prediction software Augustus78. DE analysis (DESeq2) was also performed on both reference gene expression matrices produced by RSEM. Contig identification was performed using BLASTx (e-value < 1e-10) against the NCBI nr database. Gene ontology (GO) and InterproScan annotations were done using Blast2GO79. BLASTn (P-value < 1e-5) was used to compare the de novo transcriptome and the reference transcriptome of both species.


Differently-expressed genes (DEGs) were clustered using the hclust function (cluster package) and the cutreeDynamicTree function (dynamicTreeCut package) in R. A matrix containing the fold changes of all DEGs compared in chronological order (e.g. dry-0 h, 0 h-15 m, 15 m-1 h, etc.) was used as clustering input. Expression patterns across treatments, as well as presence of candidate genes, were also used to identify clusters of interest.

cDNA synthesis for RT-qPCR

Total RNA previously isolated for RNA-seq was treated with DNase I (New England Biolabs). First strand cDNA was synthesized with SuperScript II reverse transcriptase (Invitrogen) from 0.5 μg of total RNA according to the manufacturer’s instruction using oligo (dT)18.

qPCRs were performed on a Mx3000 P qPCR system (Agilent Technologies) in a 20 μl reaction volume with 1X TaqMan Universal PCR Master Mix (Applied Biosystems), 1X SYBR green (SYBR Green I Nucleic Acid Gel Stain, Invitrogen), 250 nM of reverse and forward primers and 1 μl of cDNA template. The cycle details were as follow: initial denaturation 95 °C for 10 min, 40 cycles at 95 °C for 20 s and 60 °C for 60 s. A melting curve analysis followed the amplification cycles to examine the specificity of the reaction. Primers are summarized in Table S10. Two biological replicates were used.

The amplification efficiency was calculated using the web-based Real-Time PCR Miner algorithm ver. 4.080. Relative expression analysis of the nep-1 gene was calculated using the 2−ΔΔCT method81. Three genes (GR, PMP-3 and aaRS) reported as stable in all Globodera spp. life stages (Sabeh et al., in preparation) were used as reference for normalization. Hydrated cysts were used as calibrator treatment to calculate the fold change over the other treatments.

Data availability

Globodera rostochiensis Illumina 100 bp paired-end reads are available through NCBI under the bioproject accession number PRJNA274143. Globodera pallida Illumina sequence reads are available through the European Nucleotide Archive ( under the accession numbers ERR202482-ERR202486 (first repetition) and ERR202488-ERR202492 (second repetition). G. pallida reference transcriptome is available through the Sanger Institute (

Additional information

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This work was supported by the Agriflex program, Agriculture and Agri-Food Canada (B.M.), the National Institute for Food and Agriculture from the United States Department of Agriculture (award #2015-69004-23634) (B.M. and V.B.), the Education Spanish Ministry under the “Ayudas para la movilidad postdoctoral en centros extranjeros” scheme (J.E.P.-R.) and by the Scottish Government through the The James Hutton Institute (V.B.).

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Author notes

    • Marc-Olivier Duceppe

    Present address: Canadian Food Inspection Agency, Ottawa Laboratory Fallowfield (OLF), 3851 Fallowfield Road, Ottawa, Ontario, K2H 8P9, Canada

    • Juan Emilio Palomares-Rius

    Present address: Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Avenida Menéndez Pidal s/n, 14004 Córdoba, Campus de Excelencia Internacional Agroalimentario, ceiA3, Spain

  1. Marc-Olivier Duceppe and Joël Lafond-Lapalme contributed equally to this work.


  1. Agriculture and Agri-Food Canada, 430, Boulevard Gouin Saint-Jean-sur-Richelieu (Québec), J3B 3E6, Québec, Canada

    • Marc-Olivier Duceppe
    • , Joël Lafond-Lapalme
    • , Michaël Sabeh
    •  & Benjamin Mimee
  2. Département de Biologie, Université de Sherbrooke, Sherbrooke, J1K 2R1, Canada

    • Joël Lafond-Lapalme
    •  & Peter Moffett
  3. Cell and Molecular Sciences, The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, United Kingdom

    • Juan Emilio Palomares-Rius
    •  & Vivian Blok


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B.M., V.B. and P.M. designed experiments; M.O.D. and J.E.P.R. performed lab experiment; M.O.D. and J.L.L. carried out the bioinformatics analyses; M.S. carried out RT-qPCR work; M.O.D., J.L.L., J.E.P.R. and B.M. wrote the manuscript and all authors reviewed the manuscript.

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The authors declare that they have no competing interests.

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Correspondence to Benjamin Mimee.

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