Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Complex hierarchical microstructures of Cambrian mollusk Pelagiella: insight into early biomineralization and evolution

Abstract

Shell microstructure and mineralogy of Pelagiella madianensis Zhou & Xiao, 1984, a globally distributed Cambrian micromollusk, are investigated based on abundant and extraordinarily well-preserved specimens from Xinji Formation, Longxian, Shaanxi, North China. Five types of aragonitic microstructures have been recognized. The lamello-fibrillar microstructure, previously known from Pelagiella, constructs the outermost shell layer, while the remaining four types are reported here for the first time in this genus. They include fibrous foliated, foliated aragonite, crossed foliated lamellar and isolated tablets. The animal constructs these five types of microstructures to build its shell in a complex hierarchical pattern with four orders: crystallite columns, laths, folia and lamellae. These findings demonstrate that the capability of building complex shell microstructures had already evolved by the Cambrian explosion. In addition, this work shows that early aragonitic shells were constructed with fibers, laths, folia and isolated tablets, indicating increased controls over biomineralization by the animal.

Introduction

Molluscan shells (bivalves, gastropods and monoplacophorans) initially evolved during the earliest Cambrian (beginning at 541 Ma)1, 2 to protect the soft tissues from abiotic and biotic stress3. They were diverse in the Cambrian, both in morphology and microstructure. At least 160 genera have been described from the lower Cambrian (Terreneuvian plus Cambrian Series 2)4 and the number is still increasing5, 6. Much attention has previously been paid to the taxonomy, phylogeny, and biostratigraphy of early molluscan shells7, 8. However, micro-nano scale microstructure and the mineralogy of shells have been poorly determined. Since the pioneering work of Runnegar9, microstructures of Cambrian molluscan shells were known from phosphatic internal moulds10. Many types of microstructures found in modern molluscan shells (e.g. foliated calcite, foliated aragonite, fibrous and prismatic forms) can be traced back to the Cambrian11, indicating a rapid diversification of the lineage. Nevertheless, complex shell microstructures such as crossed lamellar and nacre have not yet been discovered in the Cambrian. In addition, the lack of detailed sub-units of already known microstructures also limits understanding of biomineralization of early molluscan shells and restricts comparisons with modern types.

One of the difficulties in determining shell microstructures of Cambrian mollusks is distinguishing and reconstructing a variety of thin layers, which appear to be preserved primarily on the surface of phosphatic internal moulds after acid-etching12, 13. The most frequently used model is the two-layered structural sequence, as Cambrian molluscan shells consisted of outer prismatic and inner lamellar layers10, 11. However, investigations into shell microstructures of living mollusks have shown that some limpet shells have up to five layers with distinctive microstructures observed in transverse section through the thickness of the shells14, 15.

Pelagiella is a globally distributed Cambrian micromollusk that has a turbospiral dextrally coiled shell and a sub-triangular aperture. Biological affinities of Pelagiella are still uncertain mainly because of the lack of anatomical evidence for torsion, and it has been interpreted as a gastropod, a paragastropod or a helcionellid16. Shell microstructure is lamello-fibrillar, which consists of fine parallel fibers arranged into a mosaic pattern (radially and comarginally oriented aragonitic crystals)11, 17. Additionally, flattened, bladed crystallites aligned with the growth direction were also reported from the inner shell layer of P. deltoides 9 and P. subangulata 3, 18. This demonstrates that their shells consist of complex microstructures, including various types of crystallites and arrangements.

Here, we document additional information on shell microstructures of P. madianensis from the Xinji Formation (equivalent to uppermost Cambrian Stage 3) of the lowermost Cambrian of the North China block19, 20, including detailed architecture through the shell. These data will be put into a general evolutionary context of early molluscan biomineralization.

Results

Mode of Preservation

The replacement of original skeletons by calcium phosphate (“phosphatization”) is of great significance in the preservation of Cambrian Small Shelly Fossils. Phosphate can produce a perfect pseudomorph and replicate very fine features of other materials, such as delicate microstructures of shells and muscular filaments of soft tissues21, 22. However, preservation of mollusks is strongly dependent on rock type, as well as on the method of fossil extraction23. Specimens are generally preserved as phosphatic internal moulds, as the calcareous shells are dissolved during the acid leaching extraction process (see Supplementary Fig. S1). Consequently, preservation of shell microstructures, especially on micro-nano scales, is extremely scarce in early molluscan shells as well as other skeletal fossils.

Most specimens of Pelagiella from the Xinji Formation that are preserved as internal moulds are composed of phosphatic spheroids (2–5 μm in diameter) (Fig. 1d–f), likely to have been formed by microbial activity. Lamellar microstructure of the shell is readily discernable and distinguishable from the internal moulds by characteristic pseudomorphic laths of aragonite, which grow consistently in a radial direction and have complex hierarchical orders (Fig. 1a–c). When magnified, some specimens show the crystallite columns stacked parallel to each other inside the lath (Fig. 1k).

Figure 1
figure1

Mode of Preservation. (a,b) LC063267, acid-etched shell residue (S) and Thin-layer (TL) of foliated aragonite, (c) LC063163, Thin layer (TL) of foliated aragonite marked by yellow dotted line, (df) LC063046, shows internal mould (I) and microbial induced spheroids, (g) LC063026, acid-etched shell residue and internal mould, (h) LC063043, amplification of outer shell surface shows the diagenetic apatite, (i) LC063522 and (j) LC063519, over-phosphatized shell remains the pseudomorph of lath-like crystals, but composed of diagenetic apatite, (k) LC063097 and (l) LC063055, crystals grow in typical prismatic form (white arrowhead). Scale bars of (a,d) are 100 μm; (b)20 μm; (c,e,g)10 μm; (f,i,k)1 μm; (h,j,l)500 nm.

The high resolution SEM images reveal a variety of nano-crystals of calcium phosphate (mainly apatite) both from phosphate coatings and shell residues. In most cases, these nanograins are 150 nm in diameter (Fig. 1i), and are irregularly arranged inside the laths, and resemble the aragonitic nanogranules of original molluscan shells (see Supplementary Fig. S2). However, nanograins are also common in the phosphate coatings (Fig. 1g,h). In these cases, each nanograin seems to be composed of smaller structures (Fig. 1j–h). Apatite crystals typically grow in a prismatic form, 50–200 nm in diameter and 350–500 nm in length (Fig. 1k,l). Consequently, it is difficult to determine whether original aragonitic nanogranules are retained during the process of phosphatization. In contrast, the apatite nanograins are very likely to be formed during diagenesis.

Shell morphology

Pelagiella madianensis has a turbospiral, dextrally coiled shell with an open umbilicus and an elongated aperture separated from the well-rounded dorsum by the depressed lateral shell (Fig. 2a,b). As a result, the shell is funnel-like in plane view and the spire is slightly submerged (Fig. 2c). Shell exterior can be finely ornamented by three distinctive sculptures with the development of the shell: smooth (Fig. 2d), knobs (Fig. 2e) and radial ridges with wrinkled ribs (Fig. 2f,g). The cross-section of shell from spire to aperture is elongated and rectangular to sub-triangular in outline.

Figure 2
figure2

Morphology and ornaments of P. madianensis. (a) LC063505 (b) lc060159 (c) lc060326 shows the turbospiral, dextrally coiled shell with an open umbilicus and elongated aperture, (d) Amplification of (a), the smooth surface of the front part, (e) LC063043, shows isolated knobs aligned in radial direction, arrow indicates radial direction, (f) LC063521 and (g) lc060138, crossed ridges with wrinkled ribs of the last whorl. Scale bars of (ac) are 100 μm; (e) 5 μm; (d,f,g) 50 μm.

Shell microstructures

The lamello-fibrillar microstructure occurs throughout the shell. It is a thin layer that is composed of continuous fibers, or bundles of fibers parallel to the shell surface, aligned in a radial direction (Fig. 3a–d). These fibers may be branched or crossed, which corresponds to the wrinkled rib sculpture (Fig. 3c). Bundles of fibers may be more or less arranged in the comarginal direction (Fig. 3e,f).

Figure 3
figure3

Lamello-fibrillar microstructure of P. madianensis. (a) lc060328 and (b) lc060145, exterior surface and interior surface of the shell with ornament of radial knobs with wrinkled ribs, (c) LC063157 and (d) LC063266, fibers of lamello-fibrillar are mainly aligned in radial direction, (e,f) LC063039 Mosaic pattern of lamello-fibrillae with fibers respectively aligned in radial and comarginal direction (double-headed arrows), white arrow in (f) indicates recrystallized fiber. Scale bars are (a) 10 μm; (be) 5 μm; (f) 1 μm.

Underlying the outermost shell layer, especially within the umbilical wall, crystals are elongated, narrow, acicular laths with straight fronts, unlike the fibers of lamello-fibrillae or the wide laths of foliated aragonite (Fig. 4a,b). These crystals have a width of about 1–2 μm and are aligned into fibrous pattern in gross appearance (Fig. 4c,d). Hence this type of microstructure is described as “fibrous foliated”.

Figure 4
figure4

Fibrous foliated microstructure of P. madianensis. (a) LC063097 (b) LC063172 (c) LC063519, the fibrous appearance and acicular laths arranged in radial direction, (d) LC063042, distinctive fibrous appearance of fibrous foliated and consistent pattern of foliated aragonite. Scale bars are (ad) 5 μm.

The foliated aragonite microstructure is widely distributed, but mainly preserved in the internal layer of the early-secreted shell. Its crystallites are always arranged in the radial direction, and three to four hierarchical orders can be identified. The second-order sheets (folia) show a stepwise pattern, margined with straight front (Fig. 5d–f). Third-order laths are parallel to each other inside the folia with width ranging from 2 to 6 μm and 50–200 nm in thickness (Figs 1k,l and 5g). Upon closer examination, numerous fourth-order crystallite columns (50–200 nm in diameter) can also be identified from each lath, which are also parallel to each other (Fig. 5d,g). The foliated aragonite shows a regular pattern unlike the fibrous foliated microstructure (Fig. 5a–c).

Figure 5
figure5

Foliated aragonite microstructure of P. madianensis. (a,b) LC063024, shows the regular pattern of foliated aragonite in radial direction, (c,d) LC060011, the second-order folia with straight front, (e,f) LC063267, stepwise second-order folia aligned in radial direction, (g) LC063522, shows the third-order laths and fourth-order crystallite columns arranged in radial direction. Scale bars are (a,c) 20 μm; (b,e) 10 μm; (d,f) 1 μm (g) 5 μm. White arrows indicate the growth (radial) direction.

Crossed foliated lamellar microstructure (CFL) is observed close to the aperture of larger specimens and shows a fourth-order hierarchical pattern from lamellae to crystallite columns. When the CFL is laid down below the foliated aragonite layer (in the direction of radial shell accretion), the orientation of laths rotate from a radial to a comarginal direction (Fig. 6a–c). Its initial layer has a width of about 2–5 μm and is composed of acicular crystallites (Fig. 6d–f). The first-order lamellae are comarginally arranged. The width of the first-order lamellae is up to 10 μm (Fig. 7a). The second-order folia and third-order laths grow in opposite directions with the angle between them (from plain view) increasing from almost 90° to 145° (Fig. 7b).

Figure 6
figure6

Initial layer of crossed foliated lamellar microstructure. (a) LC063303, the transition from foliated aragonite (FOA) to crossed foliated lamellar microstructure (CFL), (b) Amplification of (a), shows the initial layer of crossed lamellar microstructure, (c) Amplification of (a), shows the foliated aragonite microstructure, (d) LC063519, The orientation of laths rotates from radial to comarginal direction from the FOA to CFL, (e) LC063047 and (f) LC063097, The angle (from plain view) between adjacent first-order lamellae increases from almost 90 to 145°. Scale bars are (a,d) 10 μm; (b,c,e) 5 μm; (f) 1 μm. White arrows indicate the growth (radial) direction.

Figure 7
figure7

Mature crossed foliated lamellar microstructure. (a) LC063006, the first-order lamellae are secreted comarginally; folia and laths parallel growth surface, (b) Amplified SEM image shows the basic units of crystallite columns and laths arranged oppositely to adjacent first-order lamellae. Scale bar are (a) 10 μm; (b) 1 μm. White arrows indicate the growth (radial) direction.

Isolated tablets (5–10 μm in diameter) co-occur with foliated aragonite microstructure in the early-secreted shell, and are mainly restricted within the innermost shell layer (Fig. 8c). However, only the outline of the crystallites (tablets and folia) is preserved, and they do not display any detailed sub-units. The third-order laths of foliated aragonite have a pseudo-hexagonal outline with a 120° interfacial angle in contrast with the straight front of the second-order folia where space for growth is generally unlimited (Fig. 8a,b). In addition, imprints of completed hexagonal crystals can be easily identified, and the transitions of growing fronts from straight to hexagonal are gradual.

Figure 8
figure8

Isolated tablets embedded in foliated aragonite microstructure. (a) LC06–036 (b) LC06-048, SEM images show the isolated hexagonal tablet with a 120 interfacial angle, (c) LC06-085, Transition from lath or folia with straight fronts through pseudo-hexagonal fronts to hexagon outline of the crystal. Scales bars are (ac) 5 μm.

Discussion

Four hierarchical orders of shell construction (i.e. from crystallite columns to lamellae) in P. madianensis are revealed by a variety of microarchitectures and crystal orientations. In addition, five types of microstructures are also recognized in phosphatic internal moulds and phosphate-replaced shells, i.e. lamello-fibrillar, fibrous foliated, foliated aragonite, crossed foliated lamellar and isolated tablets. This demonstrates that the capability of building complex shell microstructures had already evolved by the period of the Cambrian explosion (Fig. 9).

Figure 9
figure9

Reconstruction of shell microstructures. Basic units with four hierarchical orders: lamellae, folia, laths and crystallite columns construct five types of microstructures: lamello-fibrillar, fibrous foliated, foliated aragonite, crossed foliated lamellar and isolated tablets.

The precise position of each type of microstructure is difficult to determine because boundaries or discontinuities between adjacent layers are gradational, and are particularly hard to observe on the horizontal surface of the shell. However, in selected specimens, these five types of microstructures may be preserved within specific parts of the shell (Figs 2d, 3a, 4d, 6a,d and 8c). This demonstrates that multiple layers with distinctive lamellar microstructures occur within these shells, in contrast to the classical two-layered model of this Cambrian micromolluscan species.

The lamello-fibrillar microstructure previously observed in Pelagiella subangulata Tate, 1982 constructs the outermost shell layer of P. madianensis (Fig. 2b). However, the mosaic pattern of this type of microstructure is not consistently observed in all specimens. Evidence from P. subangulata and P. deltoides shows similar crystals mainly arranged in the same radial direction9, 18. In addition, comarginal grooves on internal moulds of P. subangulata (see Bengtson et al. 1990, Fig. 169. C) seem to be composed of acicular laths in a crossed foliated lamellar pattern, rather than fibers of lamello-fibrillae. Lamello-fibrillar microstructure is observed in P. madianensis based on limited data of comarginal fibers, however additional investigation is required, particularly because detailed subunits of the fibers are unclear as a result of diagenesis. It is possible that the transition during growth from radially-arranged fibers of lamello-fibrillae to wide laths of foliated aragonite is gradual.

Narrow, elongated crystallites reinterpreted as acicular laths of fibrous foliated microstructure were previously documented from the inner shell layer of P. deltoides, but they were initially interpreted as fibers of lamello-fibrillae9. Actually, the acicular laths represent an intermediate form between fibers to laths of aragonite. As a result, the fibrous foliated microstructure is determined as a transitional stage from lamello-fibrillar to foliated aragonite in P. madianensis. This microstructure shares close similarities with the regular or irregular fibrous foliated microstructures of recent Patellogastropoda14 as both have bladed units and a fibrous appearance. However, the straight front of acicular laths in the fibrous foliated microstructures documented herein is a texture associated with aragonite rather than the calcitic arrow-like laths in Patellogastropoda.

Foliated aragonite microstructure was initially described from extant monoplacophorans24, 25, but recent work revealed that it also occurred in the Cambrian bivalves Pojetaia and Fordilla 22. In specimens studied herein, the lamellar shell layer that is formed from third-order laths may be foliated calcite26, 27 or foliated aragonite28 (see Supplementary Figs S3 and S4). They may be distinguished via their differing crystallographies, as the main surfaces of the foliated calcitic laths are {1018}27 but change to {001} in the foliated aragonite of monoplacophorans29. In addition to the different crystallographic orientations, they also show some differences in morphology. The occurrence of many groups of elongated laths with straight or rounded fronts, not toothed patterns, was proposed as foliated aragonite, as in most cases of P. madianensis.

Crossed foliated lamellar microstructure (CFL) is a new terminology to describe this unique type of shell microstructure. Determination and description are mainly based on features of its exterior surface as vertical section is inaccessible in fossil material. The microstructure studied herein is strikingly similar to foliated aragonite microstructure as both exhibit complex hierarchical growth patterns, but CFL shows a crucial difference in the growth directions of crystals. The alternative sequences of the first-order lamellae in CFL are analogous to that in the crossed lamellar (CL) and the crossed foliated (CF) microstructures of modern molluscan shells. Third-order laths of CFL are generally developed parallel to the growth surface; whereas in CL, they dip at a very high angle (see Supplementary Fig. S5). CFL and CF may be distinguished clearly via their mineralogy, as CFL is composed of aragonite, but CF is composed of calcite. Therefore, CFL exhibits straight or rounded fronts compared with arrow or tooth-like pattern in CF14.

Some of the Cambrian molluscan shells appear to have isolated tablets in their internal lamellar layer. Tablets with a rhomboidal outline are common in calcite semi-nacre30, 31, while hexagonal tablets are usually observed in some foliated aragonitic shell layers22, 32. These textures are straightforward to distinguish based on their differences in morphology and interfacial angle. The interfacial angle of hexagonal tablets is 120°, while angles of 78° and 102° occur in rhomboidal crystals11. The isolated tablet is possibly an initial developmental stage during the evolution from foliated aragonite to nacre based on their similarity in hexagonal outline of crystallites22, 33.

Examination of shell microstructures in Cambrian mollusks shows the evolutionary trend of loosely-organized lamello-fibrillae in early molluscan shells, through to the well-organized lath-like lamellar types. The lamello-fibrillae is the most widespread of mollusc shell microstructures in the early Cambrian (Terreneuvian) around the world. Commonly, crystals in early molluscan shells are loosely integrated, indicating weak control over biomineralization by organic matrices10.

By early Cambrian Stage 3, most mollusks were able to construct their shells with a variety of complex microarchitectures, e.g. foliated aragonite and isolated tablets11. The lath, blade or rod-shaped crystallites of aragonite are likely to have been derived from needle-like fibers via an intermediate stage of acicular laths as both microstructures grew in a similar lateral direction. Such laths may have eventually evolved into hexagonal tablets controlled by organic matrix. It is clear that there was a gradual transition from lamello-fibrillae to fibrous foliated microstructure in the shells of early Cambrian mollusks. Subsequently, this fibrous foliated microstructure was likely to have evolved into foliated aragonite and crossed foliated lamellar microstructure through the control of the arrangement and orientation of the third-order laths into different aggregations. This is seen in the same radial orientations of foliated aragonite, or in the comarginal but opposite orientations of crossed foliated lamellar microstructures (see Supplementary Fig. S6).

Another significant event in molluscan evolution during the early Cambrian is the biomineralization of calcite in the construction of the shell. Recently, lamellae of calcite were reported in Pelagiella from early Cambrian Parara Limestone of South Australia11. However, our work reveals that the shell of P. madianensis was exclusively made of aragonite. The origin of calcite in aragonitic shells is more difficult to decipher, but there is a clear trend marked by the transition from widespread, aragonitic mollusk shells during the early Cambrian, to evolution of calcitic shells that became common in the mid-Cambrian33. Quantitative analysis of biomaterials during the Cambrian explosion on taxa of high taxonomic rank shows a similar trend. This transition is likely to be dictated by ocean environment, in particular, seawater chemistry (Mg/Ca mole ratio), which played a major role in the initial acquisition of biomineralized skeletons34, 35. Seawater chemistry varied through the Ediacaran to Cambrian. The high mMg/Ca ratio is associated with aragonite biomineralization from Ediacaran until the Cambrian Stage 3, and the low mMg/Ca ratio with calcite biomineralization thereafter36. Mollusk data coincides well with the oscillation of seawater chemistry, suggesting that environmental changes had major influence on the evolution of biomineralization (see Supplementary Fig. S7).

The late Cambrian has a poor fossil record of mollusks. It was not until the Ordovician when strong nacreous microstructure originated in several molluscan lineages independently, i.e. bivalves, cephalopods and gastropods33, 37. By this time the main types of mineralogies and microstructures observed in modern mollusk shells had evolved. The derived crossed lamellar microstructure and nacre (mother of pearl) steadily became the two most widespread microstructures in shells of bivalves and gastropods due to their unique mechanical properties38, 39.

Methods

The Xinji Formation, yielding abundant Small Shelly Fossils (SSFs), deposited along the southwestern margin of North China block. It rests disconformably on the upper Ediacaran Dongpo Shale and is conformably overlain by the massive dolostone of the Zhushadong Formation. The specimens of Pelagiella madianensis from the Xinji Formation are mostly collected from the Chaijiawa section, Longxian County, Shaanxi, North China, approximately 0.5 km northeast to Chaijiawa village.

Rock samples were treated with buffered 10% acetic acid to retrieve acid-resistant microfossils. More than 2000 specimens of Pelagiella madianensis were recovered from the acid-resistant residues. Selected specimens were mounted, sputter-coated with gold and examined with a FEI Quanta 400 FEG scanning electron microscope at Northwest University. Approximately half of these specimens are preserved with shell microstructures. Microfossils described below are deposited at the Department of Geology, Northwest University, Xian, China.

References

  1. 1.

    Qian, Y. Taxonomy and Biostratigraphy of Small Shelly Fossils in China (ed. Qian, Y.) 216–219 (Science Press, 1999).

  2. 2.

    Qian, Y. & Bengtson, S. Palaeontology and biostratigraphy of the early Cambrian Meishucunian Stage in Yunnan Province, South China. Fossils and Strata. 24, 1–156 (1989).

    Google Scholar 

  3. 3.

    Bengtson, S. & Morris, C. S. In Origin and Early Evolution of the Metazoan (eds Lipps, J. H. & Signor, P. W.) 447–473 (Plenum Press, 1992).

  4. 4.

    Li, G. et al. Early Cambrian metazoan fossil record of South China: generic diversity and radiation patterns. Palaeoecol. 254, 229–249, doi:10.1016/j.palaeo.2007.03.017 (2007).

    Article  Google Scholar 

  5. 5.

    Parkhaev, P. Y. & Demidenko, Y. E. Zooproblematica and mollusca from the lower Cambrian Meishucun Section (Yunnan, China) and taxonomy and systematics of the Cambrian small shelly fossils of China. Paleontol J. 44, 883–1161, doi:10.1134/S0031030110080010 (2010).

    Article  Google Scholar 

  6. 6.

    Gravestock, D. I. et al. The Cambrian Biostratigraphy of the Stansbury Basin, South Australia (eds Alexander, E. M., Jago, J. B., Rozanov, A. Y. & Zhuravlev, A. Y.) 133–202 (Nauka interperiodica Press, 2001).

  7. 7.

    Parkhaev, P. Y. The Cambrian ‘basement’ of gastropod evolution. Geol Soc Spec Publ. 286, 415–421, doi:10.1144/SP286.31 (2007).

    ADS  Article  Google Scholar 

  8. 8.

    Parkhaev, P. Y. In Phylogeny and Evolution of the Mollusca (eds Ponder, W. & Lindberg, D. R.) 33–69 (University of California Press, 2008).

  9. 9.

    Runnegar, B. Shell microstructures of Cambrian molluscs replicated by phosphate. Alcheringa. 9, 245–257, doi:10.1080/03115518508618971 (1985).

    Article  Google Scholar 

  10. 10.

    Vendrasco, M. J., Porter, S. M., Kouchinsky, A. V., Li, G. & Fernandez, C. Z. Shell microstructures in early mollusks. Festivus. 42, 43–53 (2010).

    Google Scholar 

  11. 11.

    Vendrasco, M. J. et al. To infer the early evolution of mollusc shell microstructures. Key Engineering Materials. 672, 113–133, doi:10.4028/www.scientific.net/KEM.672 (2015).

    Article  Google Scholar 

  12. 12.

    Brasier, M. D. Phosphogenic events and skeletal preservation across the Precambrian-Cambrian boundary interval. Geol Soc Spec Publ. 52, 289–303, doi:10.1144/GSL.SP.1990.052.01.21 (1990).

    ADS  Article  Google Scholar 

  13. 13.

    Porter, S. M. Closing the phosphatization window: testing for the influence of taphonomic megabias on the pattern of small shelly fossil decline. Palaios. 19, 178–183 (2004).

    Article  Google Scholar 

  14. 14.

    Fuchigami, T. & Sasaki, T. The shell structure of the recent Patellogastropoda (Mollusca: Gastropoda). Paleontol Res. 9, 143–168, doi:10.2517/prpsj.9.143 (2005).

    Article  Google Scholar 

  15. 15.

    Suzuki, M. et al. Characterization of the multilayered shell of a limpet, Lottia kogamogai (Mollusca: Patellogastropoda), using SEM–EBSD and FIB–TEM techniques. J Struct Biol. 171, 223–230, doi:10.1016/j.jsb.2010.04.008 (2010).

    Article  PubMed  Google Scholar 

  16. 16.

    Kouchinsky, A. V. et al. A middle Cambrian fauna of skeletal fossils from the Kuonamka formation, northern Siberia. Alcheringa. 35, 123–189, doi:10.1080/03115518.2010.496529 (2011).

    Article  Google Scholar 

  17. 17.

    Feng, W. & Sun, W. Phosphate replicated and replaced microstructure of molluscan shells from the earliest Cambrian of China. Acta Palaeontol Pol 48, 21–30 (2003).

    Google Scholar 

  18. 18.

    Bengtson, S., Morris, S. C., Cooper, B. J., Jell, P. A. & Runnegar, B. N. Early Cambrian Fossils from South Australia (eds Bengtson, S. et al.) Association of Australasian Palaeontologists Memoir 9, 1990).

  19. 19.

    Li, L., Zhang, X., Yun, H. & Li, G. New occurrence of Cambroclavus absonus from the lowermost Cambrian of North China and its stratigraphical importance. Alcheringa. 40, 45–52, doi:10.1080/03115518.2015.1085749 (2016).

    Article  Google Scholar 

  20. 20.

    Yun, H., Zhang, X., Li, L. & Li, G. Skeletal fossils and microfacies analysis of the lowermost Cambrian in southwestern margin of North China Platform. J Asian Earth Sci. 129, 54–66, doi:10.1016/j.jseaes.2016.07.029 (2016).

    ADS  Article  Google Scholar 

  21. 21.

    Wilby, P. R. & Briggs, D. E. G. Taxonomic trends in the resolution of detail preserved in fossil phosphatized soft tissues. Geobios. 30, 493–502, doi:10.1016/S0016-6995(97)80056-3 (1997).

    Article  Google Scholar 

  22. 22.

    Vendrasco, M. J., Checa, A. G. & Kouchinsky, A. V. Shell microstructure of the early bivalve Pojetaia and the independent origin of nacre within the mollusca. Palaeontology. 54, 825–850 (2011).

    Article  Google Scholar 

  23. 23.

    Gubanov, A. P. & Peel, J. S. The early Cambrian helcionelloid mollusc Anabarella Vostokova. Palaeontology. 46, 1073–1087, doi:10.1111/pala.2003.46.issue-5 (2003).

    Article  Google Scholar 

  24. 24.

    Cruz, R., Weissmüller, G. & Farina, M. Microstructure of Monoplacophora (Mollusca) shell examined by low-voltage field emission scanning electron and atomic force microscopy. Scanning. 25, 12–18, doi:10.1002/sca.4950250104 (2003).

    Article  PubMed  Google Scholar 

  25. 25.

    Checa, A. G., Ramírez-Rico, J., González-Segura, A. & Sánchez-Navas, A. Nacre and false nacre (foliated aragonite) in extant monoplacophorans (=Tryblidiida: Mollusca). Sci Nat. 96, 111–122, doi:10.1007/s00114-008-0461-1 (2009).

    CAS  Article  Google Scholar 

  26. 26.

    Runnegar, B. Crystallography of the foliated calcite shell layers of bivalve molluscs. Alcheringa. 8, 273–290, doi:10.1080/03115518408618949 (1984).

    Article  Google Scholar 

  27. 27.

    Checa, A. G., Esteban-Delgado, F. J. & Rodríguez-Navarro, A. B. Crystallographic structure of the foliated calcite of bivalves. J Struct Biol. 157, 393–402, doi:10.1016/j.jsb.2006.09.005 (2007).

    CAS  Article  PubMed  Google Scholar 

  28. 28.

    Carter, J. G. In Skeletal Biomineralization: Patterns, Processes, and Evolutionary Trends (ed. Carter, J. G.) (Van Nostrand Press, 1990).

  29. 29.

    Checa, A. G., Sánchez-Navas, A. & Rodríguez-Navarro, A. Crystal growth in the foliated aragonite of monoplacophorans (Mollusca). Cryst Growth Des. 9, 4574–4580, doi:10.1021/cg9005949 (2009).

    CAS  Article  Google Scholar 

  30. 30.

    Vendrasco, M. J. & Checa, A. G. Shell microstructure and its inheritance in the calcitic helcionellid Mackinnonia. Est J Earth Sci 64, 99–104, doi:10.3176/earth.2015.18 (2015).

    Article  Google Scholar 

  31. 31.

    Vendrasco, M. J., Porter, S. M., Kouchinsky, A. V., Li, G. & Fernandez, C. Z. New data on molluscs and their shell microstructures from the Middle Cambrian Gowers Formation, Australia. Palaeontology. 53, 97–135, doi:10.1111/pala.2010.53.issue-1 (2010).

    Article  Google Scholar 

  32. 32.

    Kouchinsky, A. V., Bengtson, S., Clausen, S. & Vendrasco, M. J. An early Cambrian fauna of skeletal fossils from the Emyaksin Formation, northern Siberia. Acta Palaeontol Pol 60, 421–512 (2015).

    Google Scholar 

  33. 33.

    Vendrasco, M. J., Checa, A. G., Heimbrock, W. P. & Baumann, S. D. Nacre in Molluscs from the Ordovician of the Midwestern United States. Geosciences 3, 1–29, doi:10.3390/geosciences3010001 (2013).

    ADS  Article  Google Scholar 

  34. 34.

    Porter, S. M. Seawater chemistry and early carbonate biomineralization. Science. 316, 1302–1302, doi:10.1126/science.1137284 (2007).

    ADS  CAS  Article  PubMed  Google Scholar 

  35. 35.

    Porter, S. M. Calcite and aragonite seas and the de novo acquisition of carbonate skeletons. Geobiology 8, 256–277, doi:10.1111/j.1472-4669.2010.00246.x (2010).

    CAS  Article  PubMed  Google Scholar 

  36. 36.

    Wood, R. & Zhuravlev, A. Y. Escalation and ecological selectively of mineralogy in the Cambrian Radiation of skeletons. Earth-Sci Rev 115, 249–261, doi:10.1016/j.earscirev.2012.10.002 (2012).

    ADS  CAS  Article  Google Scholar 

  37. 37.

    Mutvei, H. Flexible nacre in the nautiloid Isorthoceras, with remarks on the evolution of cephalopod nacre. Lethaia. 16, 233–240, doi:10.1111/let.1983.16.issue-3 (1983).

    Article  Google Scholar 

  38. 38.

    Carter, J. G. et al. Part N, Revised, Volume 1, Chapter 31: Illustrated Glossary of the Bivalvia. (ed. Carter, J. G.) (The University of Kansas, Paleontological Institute 2012).

  39. 39.

    Dauphin, Y. et al. Microstructure, nanostructure and composition of the shell of Concholepas concholepas, (Gastropoda, Muricidae). Aquat Living Resour 16, 95–103, doi:10.1016/S0990-7440(03)00022-6 (2003).

    Article  Google Scholar 

Download references

Acknowledgements

We are grateful for Zhifei Zhang for valuable discussions and suggestions, Cong Liu, Wei Liu, Wenrui Pei, Xin Wang and Yuan Zhang for assistances in the field and fossil preparations. This work was supported by the National Basic Research Program of China (Grant No. 2013CB835002), Natural Science Foundation of China (Grant Nos 41621003 and 41372021).

Author information

Affiliations

Authors

Contributions

X.Z. designed the research. L.L. and X.Z. conceived and prepared the manuscripts. H.Y. contributed to fossils collection and fieldwork. G.L. provided input in the analysis and interpretation. All authors contributed to subsequent revisions.

Corresponding author

Correspondence to Xingliang Zhang.

Ethics declarations

Competing Interests

The authors declare that they have no competing interests.

Additional information

Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Li, L., Zhang, X., Yun, H. et al. Complex hierarchical microstructures of Cambrian mollusk Pelagiella: insight into early biomineralization and evolution. Sci Rep 7, 1935 (2017). https://doi.org/10.1038/s41598-017-02235-9

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links