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Mammalian CCR4–NOT binds and ubiquitinates 80S ribosomes to enforce translational stalling

Nature Structural & Molecular Biology volume 30, pages 1254–1255 (2023)Cite this article

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In vitro reconstitution of recognition of 80S ribosomes by CCR4–NOT, cryo-electron microscopy (cryoEM), crosslinking mass spectrometry and biochemical characterization reveal that CCR4–NOT specifically identifies ribosomes stalled during translation elongation. CCR4–NOT occupies the ribosomal exit site (E site) and locks the ribosomal L1 stalk in an open conformation to enforce the stalled state.

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Fig. 1: Structure of the CCR4–NOT-bound mammalian 80S ribosome.

References

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This is a summary of: Absmeier, E. et al. Specific recognition and ubiquitination of translating ribosomes by mammalian CCR4–NOT. Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-023-01075-8 (2023).

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Mammalian CCR4–NOT binds and ubiquitinates 80S ribosomes to enforce translational stalling. Nat Struct Mol Biol 30, 1254–1255 (2023). https://doi.org/10.1038/s41594-023-01079-4

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