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NANOSCALE BIOPHYSICS

Stretching the resolution limit of atomic force microscopy

Atomic force microscopy (AFM) is unique in visualizing functional biomolecules in aqueous solution at ~1 nm resolution. By borrowing localization methods from fluorescence microscopy, AFM has been shown to discern structural domains that may be separated by only a few Ångströms.

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Fig. 1: Factors that can limit spatial resolution in bio-AFM.
Fig. 2: From peaks to peaking probability and LAFM.

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Correspondence to Bart W. Hoogenboom.

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Hoogenboom, B.W. Stretching the resolution limit of atomic force microscopy. Nat Struct Mol Biol 28, 629–630 (2021). https://doi.org/10.1038/s41594-021-00638-x

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