We developed, characterized and validated nLight sensors, a new family of genetically encoded green and red fluorescent norepinephrine indicators based on an alpha-1 adrenergic receptor. nLight probes can detect norepinephrine in living animals with superior sensitivity, ligand specificity and temporal resolution as compared with previous tools.
This is a preview of subscription content, access via your institution
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$29.99 / 30 days
cancel any time
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Patriarchi, T. et al. Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors. Science 360, eaat4422 (2018). This paper introduced the first dopamine sensors for in vivo imaging.
Feng, J. et al. A genetically encoded fluorescent sensor for rapid and specific in vivo detection of norepinephrine. Neuron 102, 745–761.e8 (2019). This paper describes first-generation norepinephrine sensors.
Patriarchi, T. et al. An expanded palette of dopamine sensors for multiplex imaging in vivo. Nat. Methods 17, 1147–1155 (2020). This paper reports the first red fluorescent dopamine sensor for in vivo imaging.
Labouesse, M. A. & Patriarchi, T. A versatile GPCR toolkit to track in vivo neuromodulation: not a one-size-fits-all sensor. Neuropsychopharmacology 46, 2043–2047 (2021). This review article describes advantages and caveats of neuromodulator sensors.
Sabatini, B. L. & Tian, L. Imaging neurotransmitter and neuromodulator dynamics in vivo with genetically encoded indicators. Neuron 108, 17–32 (2020). This review article presents an overview of protein-based tools for imaging neurotransmitters.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This is a summary of: Kagiampaki, Z. et al. Sensitive multicolor indicators for monitoring norepinephrine in vivo. Nat. Methods https://doi.org/10.1038/s41592-023-01959-z (2023).
Rights and permissions
About this article
Cite this article
Ultrafast nLight indicators for sensitive and specific in vivo imaging of norepinephrine. Nat Methods 20, 1289–1290 (2023). https://doi.org/10.1038/s41592-023-01960-6
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41592-023-01960-6
