We developed CREST (CRISPR editing-based lineage-specific tracing) to enable high-throughput mapping of single-cell lineages in any Cre lineage of interest in mice. In addition, we delineated a comprehensive lineage landscape of the developing mouse ventral midbrain, revealing novel differentiation trajectories and molecular programs underlying neural specification.
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References
Bandler, R. C. et al. Single-cell delineation of lineage and genetic identity in the mouse brain. Nature 601, 404–409 (2022). This paper reports the first application of single-cell barcoding-based lineage tracing in mouse brain.
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Weinreb, C., Rodriguez-Fraticelli, A., Camargo, F. D. & Klein, A. M. Lineage tracing on transcriptional landscapes links state to fate during differentiation. Science 367, eaaw3381 (2020). This paper reports a clonal splitting strategy to simultaneously acquire both transcriptomic and lineage information of single cells.
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This is a summary of: Xie, L. et al. Comprehensive spatiotemporal mapping of single-cell lineages in developing mouse brain by CRISPR-based barcoding. Nat. Methods https://doi.org/10.1038/s41592-023-01947-3 (2023).
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A method to map single-cell lineages in the mouse brain by CRISPR-based barcoding. Nat Methods 20, 1139–1140 (2023). https://doi.org/10.1038/s41592-023-01948-2
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DOI: https://doi.org/10.1038/s41592-023-01948-2
