Cell type-specific transgene expression in mice has broad utility in biomedical research. We developed a versatile system for in vivo transgene delivery using adeno-associated virus (AAV). Efficient and tissue-specific transgene expression is achieved by regulating the expression of the gene encoding the AAV receptor, thereby precisely targeting AAV to the cell type of interest.
This is a preview of subscription content, access via your institution
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$29.99 / 30 days
cancel any time
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Gurumurthy, C. B., Saunders, T. L. & Ohtsuka, M. Designing and generating a mouse model: frequently asked questions. J. Biomed. Res. 35, 76–90 (2021). This paper shows traditional methods of generating transgenic mouse models.
Haery, L. et al. Adeno-associated virus technologies and methods for targeted neuronal manipulation. Front. Neuroanat. 13, 93 (2019). This paper shows how AAV can be used to target certain cells, with a focus on neuronal targeting.
Pillay, S. et al. An essential receptor for adeno-associated virus infection. Nature 530, 108–112 (2016). This paper identified the receptor for AAV and other host factors important for viral entry and gene expression.
Chan, K. Y. et al. Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous systems. Nat. Neurosci. 20, 1172–1179 (2017). This paper identifies AAV capsid variants that can target the nervous system — specifically, the one that can cross the blood–brain barrier that we used in our study.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This is a summary of: Zengel, J. et al. Hardwiring tissue-specific AAV transduction in mice through engineered receptor expression. Nat. Methods https://doi.org/10.1038/s41592-023-01896-x (2023).
Rights and permissions
About this article
Cite this article
A flexible system for tissue-specific gene expression in mice using adeno-associated virus. Nat Methods 20, 997–998 (2023). https://doi.org/10.1038/s41592-023-01897-w
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41592-023-01897-w
