Simultaneous maximization of sensitivity, data completeness and throughput in mass-spectrometry proteomics often necessitates trade-offs. To mitigate these trade-offs, we introduce a prioritization algorithm that achieves high sensitivity and data completeness while maximizing throughput. With prioritized single-cell proteomics (pSCoPE), we consistently and accurately quantify proteins and their post-translational modifications in single macrophages and link them to endocytic activity.
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This is a summary of: Huffman, R. G. et al. Prioritized mass spectrometry increases the depth, sensitivity and data completeness of single-cell proteomics. Nat. Methods https://doi.org/10.1038/s41592-023-01830-1 (2023).
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Extending the sensitivity, consistency and depth of single-cell proteomics. Nat Methods 20, 649–650 (2023). https://doi.org/10.1038/s41592-023-01786-2